Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr6

Orthogroup ID

OG0005339

Physical Location & Seq

Genomic Coordinates Reverse (-)

20764931 .. 20767546

Gene Structure 2616 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom06g16530.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2616 bp

ATGTTGCGAGCAAAGCAATTAAGTAACCTTTCAAGTAGTGCGAGGTCATTCTTCCTTACTGGGCCACGATGTAGTGCGACGGATGGAAATTCATGCTCTTGCTCTGAAGATGAAACTTGTGTTTCACAAAGGCAGCTAGCAAGAGATGGAGGTCTACTTGCCCAAAATCCATCCACCATGGTATCCAAACCTTCAGCAAGGGCAGGAACCATCCTCTTGGGAGATGCAGTAAAAGTGGCAGGTCCTCGTAAAGCTGGAAGTGTTGACCATACAGCTTCTATTAATCAGGTTGCAACAGCACCAAGATCTTTTGGGAGATCGGAGACTGTAAGTTATGCTAGTAGCACGGATGTGGTGCACTCATCACCTCTTGTTGCAGACCAGTTTGCTAAGGCTGGAGTTGCAGCTGTAAATTTTCTGTCTGATATAGTAAATGGCAAGCTTCCTTTATCTGATGGGCTTGGAATCCTTAATAACTGTATGGTTGATCCGACCAGGCCCCTCAACGGTATCAAGCCATCACATGTGAAACAAATAAAAAGAGAACATTTCACTAGTGGTCGTCCAAAACCAGCTACTGAGGTATCAGCTGCATCAAAGCCTACAAGTAATAATCATGGTCCAAAGGGCAAAGGTGAGAAATCCAATTTCGTTAAAGGTCTCAATCATGTTCCATATACTAGGACGGAAAGCTCAGCAGCACCTCATACTGTATACTCGGATCATCATGAGCAAAGGTCTATGCCACCGAAATCAAAACCTCATTCAAACTTCATTCCAAATTACAGTTCCAGTGCGCAGACCTCCGATGCAGAGACCATGGGTCATGCAACTCATGCCACTAAAAGTTTCAACAGGCCCATAAGAGATGTGAAAATGCCAACAGGAATAGCTCGCCAAATCAACAGACAATTTGCTCACACTGGGAATGTTGTTCATAATGTTTCTCACATACTGCAACAGACACGATGGGGTCCTGCTGCAGAGGCCGCTATTGAAAATCTCAACTGTTCAATGGATGCATATCAAGCAAACCAAATTTTGAAGCAACTTCAAGATCGATCTGTTGCGCTTGGATTTTTTTATTGGCTTAAGCGGCAAGCAGGATTCAAGCATGATGGGCACACTTATACCACCATGGTTGGATCGATCCTTGGTCGTTCCAGGCAGTTTGGTGCCATTAACAAGTTGCTTAATCAGATGGTGAAAGAAGGATGCCAGCCAAATGTTGTGACATATAACCGTCTGATTCATAGTTATGGCCGTGCAAACTATTTGAAAGAAGCAATGAATGTTTTCAATCAAATGCAAGAAGCGGGATGTGAACCTGACCGTGTTACCTACTCCACACTAATTGACATCCATGCAAAAGCTGGATTTCTTGACGTTGCCCTAGGACTATATGACAAGATGCAAGAGGCTGGTCTTGCTCCCGACACATTCACTTACAGTGTTATGATCAACTGTCTTGGGAAAGCTGGTCATTTAGATGCTGCTCATCGGCTATTTTGTGAGATGGTTAATCATGGTTGTGTTCCCAATTTGGTCACCTACAACATCATGATAGCTTTGCAGGCCAAGGCAAGGAATTATGAGACTTCTTTAAAGCTCTACCGTGACATGCAGGGTGCGGGATTTGAACCGGATAAGGTAACTTATAGCATAGTAATGGAGGTCCTTGGCCACTGTGGATATCTTGAGGAAGCTGAGGCGATTTTTCATGAAATGAAACGGAAAAACTGGGTGCCTGATGAGCCTGTTTATGGTCTTTTGGTAGATTTGTGGGGCAAGGCTGGTAACATAGAAAAAGCCTGGAGTTGGTATCAAGCTATGCTCCATGCAGGTTTACGCCCTAATGTGCCTACTTGCAATTCTCTGCTCAGTGCGTTTCTTAGGGTGCACCAGCTGTCCGATGCCTATAACTTGCTGCAGAGCATGATGGGTTTAGGTCTAAACCCTTCTTTGCAGACATATACATTGCTTCTCAGTTGTTGCACAGAAGCACAATCACCATATGACATGGGATTTTGTGGTGACCTGATGGCAGTCACAGGCCATCCTGCACATAAATTCTTACTGTCTATGCCGTCAGCAGGACCTGATGGTCAAAACGTGCGAGAGCACATGAGCCGGTTCTTGGATATGATGCATAGCGAGGATAGAGAAAGCAAGAGGGGGCTTGTAGATGCAGTAGTTGACTTTCTTCACAAGGCAGGACTAAAGGAGGAGGCGGGTTCAGTCTGGGAAGTGGCTGCACAAAAGAATGTCTATCCGGACGCCATCAAAGAGAAAAGCTCATGTTATTGGCTTATTAACCTGCACGTCATGTCTGACGGTACTGCTGTGATAGCTCTATCAAGAACGCTCGCATGGTTTCGCCAACAGATGCTAATTTCCGGGATTTGTCCAAGCCGGATTGATATTGTGACAGGATGGGGCCGGCGGAGTAGGGTTACAGGATCATCTTTGGTTAGGCAGGCAGTGCAGGAACTGCTGAATGTTTTTCGCTTCCCGTTCTTCACTGAAAACGGCAATTCTGGTTGTTTTGTGGGATGTGGGGAGCCTCTTAACAGGTGGTTGCTCCAATCTTATGTGGAGCGAATGCATTTATTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

872

Amino Acids

95.91

Weight (kDa)

8.81

Isoelectric Point (pI)

40.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_3	PF13812	368 - 421	5.5e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	375 - 414	1.1e-06	PPR repeat family
PPR_3	PF13812	396 - 454	6.7e-14	Pentatricopeptide repeat domain
PPR_1	PF12854	405 - 437	2.8e-11	PPR repeat
PPR_2	PF13041	408 - 456	2.6e-17	PPR repeat family
PPR	PF01535	411 - 441	9.1e-08	PPR repeat
PPR_1	PF12854	440 - 472	2.5e-08	PPR repeat
PPR	PF01535	446 - 476	2.1e-06	PPR repeat
PPR_2	PF13041	446 - 492	5.2e-12	PPR repeat family
PPR_long	PF17177	462 - 560	3.1e-07	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	467 - 523	5.4e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	475 - 507	3.4e-09	PPR repeat
PPR	PF01535	481 - 511	1.8e-07	PPR repeat
PPR_2	PF13041	486 - 522	1.5e-11	PPR repeat family
PPR_2	PF13041	513 - 560	1.1e-11	PPR repeat family
PPR	PF01535	551 - 580	5e-06	PPR repeat
PPR_long	PF17177	567 - 668	1.1e-06	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	611 - 665	2.8e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	618 - 665	7.6e-09	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005339)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18900 AT1G18900 AT1G18900 AT1G18900
fragaria_vesca	FvH4_5g15730 FvH4_5g15730 FvH4_5g15730
malus_domestica	MD06G1185900.v1.1 MD14G1192100.v1.1
prunus_persica	Prupe.5G184700_v2.0.a1 Prupe.5G184700_v2.0.a1 Prupe.5G184700_v2.0.a1 Prupe.5G184700_v2.0.a1 Prupe.5G184700_v2.0.a1
pyrus_communis	pycom06g16530 pycom14g15980
rosa_chinensis	RchiOBHm_Chr7g0180381
rosa_laevigata	RLG00000005272
rosa_multiflora	Rmu_sc0003526.1_g000009
rosa_roxburghii	Rroxscaffold_3G00272940
rosa_rugosa	Rorug06G0432600 Rorug06G0432700 Rorug06G0432800 Rorug06G0432800
rosa_samantha	Rh7AG033200 Rh7BG033100 Rh7CG035000 Rh7DG033600
rosa_wichuraiana	Rw7G002770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2103
Acc16I	TGCGCA	1 cut(s)	798
Acc36I	ACCTGC	3 cut(s)	230, 1832, 2325
AccB1I	GGYRCC	2 cut(s)	1175, 1744
AccI	GTMKAC	2 cut(s)	154, 714
AccIII	TCCGGA	1 cut(s)	2272
AciI	CCGC	6 cut(s)	990, 1096, 1316, 1631, 2231, 2443
AclWI	GGATC	5 cut(s)	482, 729, 1144, 1153, 2467
AcoI	YGGCCR	2 cut(s)	1261, 1681
AcsI	RAATTY	4 cut(s)	88, 412, 1038, 2069
AcuI	CTGAAG	2 cut(s)	126, 177
AcyI	GRCGYC	1 cut(s)	2277
AfaI	GTAC	1 cut(s)	2338
AfiI	CCNNNNNNNGG	6 cut(s)	506, 1584, 1763, 1818, 2093, 2137
AflII	CTTAAG	1 cut(s)	1091
AflIII	ACRYGT	1 cut(s)	523
AhdI	GACNNNNNGTC	1 cut(s)	822
AhlI	ACTAGT	1 cut(s)	554
AjiI	CACGTC	1 cut(s)	2323
AjnI	CCWGG	3 cut(s)	494, 1163, 1811
AjuI	GAANNNNNNNTTGG	2 cut(s)	772, 804
AleI	CACNNNNGTG	1 cut(s)	350
Alw21I	GWGCWC	3 cut(s)	360, 1902, 2124
Alw26I	GTCTC	4 cut(s)	317, 665, 809, 1589
Alw44I	GTGCAC	2 cut(s)	356, 1898
AlwI	GGATC	5 cut(s)	482, 729, 1144, 1153, 2467
AlwNI	CAGNNNCTG	3 cut(s)	578, 2099, 2491
Aor13HI	TCCGGA	1 cut(s)	2272
AoxI	GGCC	9 cut(s)	62, 497, 857, 987, 1261, 1575, 1681, 2053, 2437
ApaLI	GTGCAC	2 cut(s)	356, 1898
ApeKI	GCWGC	8 cut(s)	133, 404, 590, 698, 980, 1493, 1927, 2252
ApoI	RAATTY	4 cut(s)	88, 412, 1038, 2069
AseI	ATTAAT	1 cut(s)	282
Asp700I	GAANNNNTTC	1 cut(s)	1295
AspA2I	CCTAGG	1 cut(s)	1393
AspLEI	GCGC	2 cut(s)	799, 1072
AspS9I	GGNCC	9 cut(s)	62, 242, 498, 620, 858, 974, 1675, 2096, 2437
AsuC2I	CCSGG	1 cut(s)	2398
AsuHPI	GGTGA	6 cut(s)	357, 647, 1216, 1540, 2001, 2045
AsuNHI	GCTAGC	1 cut(s)	136
AvaII	GGWCC	5 cut(s)	242, 620, 974, 1675, 2096
AvrII	CCTAGG	1 cut(s)	1393
BaeGI	GKGCMC	3 cut(s)	360, 1125, 1902
BaeI	ACNNNNGTAYC	6 cut(s)	165, 198, 567, 600, 2328, 2361
BalI	TGGCCA	1 cut(s)	1683
BanI	GGYRCC	2 cut(s)	1175, 1744
Bbv12I	GWGCWC	3 cut(s)	360, 1902, 2124
BbvCI	CCTCAGC	1 cut(s)	1707
BbvI	GCAGC	8 cut(s)	145, 416, 577, 710, 967, 1480, 1914, 2239
BceAI	ACGGC	3 cut(s)	1248, 2071, 2545
BciT130I	CCWGG	3 cut(s)	496, 1165, 1813
BciVI	GTATCC	1 cut(s)	193
BclI	TGATCA	1 cut(s)	1458
BcnI	CCSGG	1 cut(s)	2398
BcoDI	GTCTC	4 cut(s)	317, 665, 809, 1589
BcuI	ACTAGT	1 cut(s)	554
BfaI	CTAG	5 cut(s)	137, 339, 555, 681, 1394
BfmI	CTRYAG	2 cut(s)	981, 1928
BfrI	CTTAAG	1 cut(s)	1091
BfuAI	ACCTGC	3 cut(s)	230, 1832, 2325
BfuI	GTATCC	1 cut(s)	193
BglII	AGATCT	1 cut(s)	305
BlnI	CCTAGG	1 cut(s)	1393
BlpI	GCTNAGC	1 cut(s)	694
Bme1390I	CCNGG	4 cut(s)	496, 1165, 1813, 2398
Bme18I	GGWCC	5 cut(s)	242, 620, 974, 1675, 2096
BmeRI	GACNNNNNGTC	1 cut(s)	822
BmgBI	CACGTC	1 cut(s)	2323
BmgT120I	GGNCC	9 cut(s)	62, 242, 498, 620, 858, 974, 1675, 2096, 2437
BmiI	GGNNCC	7 cut(s)	208, 500, 975, 1177, 1746, 2438, 2562
BmrFI	CCNGG	4 cut(s)	496, 1165, 1813, 2398
BmrI	ACTGGG	3 cut(s)	69, 933, 1750
BmtI	GCTAGC	1 cut(s)	140
BmuI	ACTGGG	3 cut(s)	69, 933, 1750
BplI	GAGNNNNNCTC	2 cut(s)	2279, 2311
BpmI	CTGGAG	2 cut(s)	417, 1834
Bpu10I	CCTNAGC	2 cut(s)	390, 1707
Bpu1102I	GCTNAGC	1 cut(s)	694
BpuEI	CTTGAG	1 cut(s)	1718
BpuMI	CCSGG	1 cut(s)	2398
Bsa29I	ATCGAT	2 cut(s)	1060, 1148
BsaBI	GATNNNNATC	1 cut(s)	1023
BsaHI	GRCGYC	1 cut(s)	2277
BsaI	GGTCTC	2 cut(s)	665, 809
BsaJI	CCNNGG	7 cut(s)	177, 819, 1137, 1153, 1393, 1578, 1678
BsaWI	WCCGGW	2 cut(s)	1642, 2272
BsaXI	ACNNNNNCTCC	2 cut(s)	213, 243
Bsc4I	CCNNNNNNNGG	6 cut(s)	506, 1584, 1763, 1818, 2093, 2137
Bse118I	RCCGGY	2 cut(s)	2130, 2439
Bse1I	ACTGG	5 cut(s)	64, 382, 792, 928, 1745
Bse3DI	GCAATG	2 cut(s)	1293, 1976
Bse8I	GATNNNNATC	1 cut(s)	1023
BseAI	TCCGGA	1 cut(s)	2272
BseBI	CCWGG	3 cut(s)	496, 1165, 1813
BseCI	ATCGAT	2 cut(s)	1060, 1148
BseDI	CCNNGG	7 cut(s)	177, 819, 1137, 1153, 1393, 1578, 1678
BseJI	GATNNNNATC	1 cut(s)	1023
BseLI	CCNNNNNNNGG	6 cut(s)	506, 1584, 1763, 1818, 2093, 2137
BseMI	GCAATG	2 cut(s)	1293, 1976
BseMII	CTCAG	5 cut(s)	570, 708, 1698, 1894, 1999
BseNI	ACTGG	5 cut(s)	64, 382, 792, 928, 1745
BseRI	GAGGAG	1 cut(s)	2240
BseSI	GKGCMC	3 cut(s)	360, 1125, 1902
BseXI	GCAGC	8 cut(s)	145, 416, 577, 710, 967, 1480, 1914, 2239
BsgI	GTGCAG	4 cut(s)	2046, 2238, 2303, 2504
BshFI	GGCC	9 cut(s)	64, 499, 859, 989, 1263, 1577, 1683, 2055, 2439
BshNI	GGYRCC	2 cut(s)	1175, 1744
BshVI	ATCGAT	2 cut(s)	1060, 1148
BsiHKAI	GWGCWC	3 cut(s)	360, 1902, 2124
BsiSI	CCGG	6 cut(s)	1643, 2131, 2273, 2397, 2413, 2440
BslI	CCNNNNNNNGG	6 cut(s)	506, 1584, 1763, 1818, 2093, 2137
BsmAI	GTCTC	4 cut(s)	317, 665, 809, 1589
BsmI	GAATGC	1 cut(s)	2607
BsnI	GGCC	9 cut(s)	64, 499, 859, 989, 1263, 1577, 1683, 2055, 2439
Bso31I	GGTCTC	2 cut(s)	665, 809
Bsp1286I	GDGCHC	4 cut(s)	360, 1125, 1902, 2124
Bsp13I	TCCGGA	1 cut(s)	2272
Bsp1720I	GCTNAGC	1 cut(s)	694
Bsp19I	CCATGG	3 cut(s)	177, 819, 1137
BspACI	CCGC	6 cut(s)	990, 1096, 1316, 1631, 2231, 2443
BspANI	GGCC	9 cut(s)	64, 499, 859, 989, 1263, 1577, 1683, 2055, 2439
BspCNI	CTCAG	5 cut(s)	571, 707, 1699, 1893, 1998
BspDI	ATCGAT	2 cut(s)	1060, 1148
BspEI	TCCGGA	1 cut(s)	2272
BspHI	TCATGA	3 cut(s)	727, 1560, 1720
BspLI	GGNNCC	7 cut(s)	208, 500, 975, 1177, 1746, 2438, 2562
BspMAI	CTGCAG	2 cut(s)	985, 1932
BspMI	ACCTGC	3 cut(s)	230, 1832, 2325
BspOI	GCTAGC	1 cut(s)	140
BspPI	GGATC	5 cut(s)	482, 729, 1144, 1153, 2467
BspT107I	GGYRCC	2 cut(s)	1175, 1744
BspTI	CTTAAG	1 cut(s)	1091
BspTNI	GGTCTC	2 cut(s)	665, 809
BsrDI	GCAATG	2 cut(s)	1293, 1976
BsrFI	RCCGGY	2 cut(s)	2130, 2439
BsrI	ACTGG	5 cut(s)	64, 382, 792, 928, 1745
BssAI	RCCGGY	2 cut(s)	2130, 2439
BssECI	CCNNGG	7 cut(s)	177, 819, 1137, 1153, 1393, 1578, 1678
BssNAI	GTATAC	1 cut(s)	715
BssNI	GRCGYC	1 cut(s)	2277
BssT1I	CCWWGG	7 cut(s)	177, 819, 1137, 1153, 1393, 1578, 1678
Bst1107I	GTATAC	1 cut(s)	715
Bst2UI	CCWGG	3 cut(s)	496, 1165, 1813
BstACI	GRCGYC	1 cut(s)	2277
BstAFI	CTTAAG	1 cut(s)	1091
BstAPI	GCANNNNNTGC	3 cut(s)	836, 2482, 2491
BstDEI	CTNAG	7 cut(s)	390, 579, 694, 1707, 1880, 1892, 1985
BstDSI	CCRYGG	3 cut(s)	177, 819, 1137
BstEII	GGTNACC	2 cut(s)	1546, 2033
BstHHI	GCGC	2 cut(s)	799, 1072
BstMAI	GTCTC	4 cut(s)	317, 665, 809, 1589
BstNI	CCWGG	3 cut(s)	496, 1165, 1813
BstNSI	RCATGY	2 cut(s)	527, 1624
BstPI	GGTNACC	2 cut(s)	1546, 2033
BstSCI	CCNGG	4 cut(s)	494, 1163, 1811, 2396
BstSFI	CTRYAG	2 cut(s)	981, 1928
BstSLI	GKGCMC	3 cut(s)	360, 1125, 1902
BstV1I	GCAGC	8 cut(s)	145, 416, 577, 710, 967, 1480, 1914, 2239
BstX2I	RGATCY	1 cut(s)	305
BstXI	CCANNNNNNTGG	1 cut(s)	217
BstYI	RGATCY	1 cut(s)	305
BstZ17I	GTATAC	1 cut(s)	715
Bsu15I	ATCGAT	2 cut(s)	1060, 1148
BsuI	GTATCC	1 cut(s)	193
BsuRI	GGCC	9 cut(s)	64, 499, 859, 989, 1263, 1577, 1683, 2055, 2439
BsuTUI	ATCGAT	2 cut(s)	1060, 1148
BtgI	CCRYGG	3 cut(s)	177, 819, 1137
BtrI	CACGTC	1 cut(s)	2323
BtsI	GCAGTG	1 cut(s)	2487
BtsIMutI	CAGTG	7 cut(s)	799, 921, 1456, 1684, 1888, 2487, 2520
BveI	ACCTGC	3 cut(s)	230, 1832, 2325
CaiI	CAGNNNCTG	3 cut(s)	578, 2099, 2491
CciI	TCATGA	3 cut(s)	727, 1560, 1720
CfoI	GCGC	2 cut(s)	799, 1072
Cfr10I	RCCGGY	2 cut(s)	2130, 2439
Cfr13I	GGNCC	9 cut(s)	62, 242, 498, 620, 858, 974, 1675, 2096, 2437
ClaI	ATCGAT	2 cut(s)	1060, 1148
CseI	GACGC	1 cut(s)	2285
Csp6I	GTAC	1 cut(s)	2337
CspCI	CAANNNNNGTGG	4 cut(s)	1123, 1158, 1336, 1371
CviQI	GTAC	1 cut(s)	2337
DdeI	CTNAG	7 cut(s)	390, 579, 694, 1707, 1880, 1892, 1985
DraI	TTTAAA	1 cut(s)	1605
DrdI	GACNNNNNNGTC	1 cut(s)	2103
DriI	GACNNNNNGTC	1 cut(s)	822
DseDI	GACNNNNNNGTC	1 cut(s)	2103
EaeI	YGGCCR	2 cut(s)	1261, 1681
Eam1105I	GACNNNNNGTC	1 cut(s)	822
EciI	GGCGGA	1 cut(s)	2458
Eco130I	CCWWGG	7 cut(s)	177, 819, 1137, 1153, 1393, 1578, 1678
Eco31I	GGTCTC	2 cut(s)	665, 809
Eco32I	GATATC	1 cut(s)	1694
Eco47I	GGWCC	5 cut(s)	242, 620, 974, 1675, 2096
Eco57I	CTGAAG	2 cut(s)	126, 177
Eco91I	GGTNACC	2 cut(s)	1546, 2033
EcoO109I	RGGNCCY	5 cut(s)	242, 498, 974, 1675, 2096
EcoO65I	GGTNACC	2 cut(s)	1546, 2033
EcoRII	CCWGG	3 cut(s)	494, 1163, 1811
EcoRV	GATATC	1 cut(s)	1694
EcoT14I	CCWWGG	7 cut(s)	177, 819, 1137, 1153, 1393, 1578, 1678
EcoT22I	ATGCAT	3 cut(s)	1024, 2151, 2607
ErhI	CCWWGG	7 cut(s)	177, 819, 1137, 1153, 1393, 1578, 1678
FauI	CCCGC	3 cut(s)	1309, 1624, 2224
FauNDI	CATATG	1 cut(s)	2014
FbaI	TGATCA	1 cut(s)	1458
FblI	GTMKAC	2 cut(s)	154, 714
FspBI	CTAG	5 cut(s)	137, 339, 555, 681, 1394
FspI	TGCGCA	1 cut(s)	798
GlaI	GCGC	2 cut(s)	798, 1071
GsuI	CTGGAG	2 cut(s)	417, 1834
HaeIII	GGCC	9 cut(s)	64, 499, 859, 989, 1263, 1577, 1683, 2055, 2439
HapII	CCGG	6 cut(s)	1643, 2131, 2273, 2397, 2413, 2440
HgaI	GACGC	1 cut(s)	2285
HhaI	GCGC	2 cut(s)	799, 1072
Hin1I	GRCGYC	1 cut(s)	2277
Hin6I	GCGC	2 cut(s)	797, 1070
HinP1I	GCGC	2 cut(s)	797, 1070
HincII	GTYRAC	2 cut(s)	265, 2197
HindII	GTYRAC	2 cut(s)	265, 2197
HindIII	AAGCTT	1 cut(s)	440
HinfI	GANTC	3 cut(s)	465, 1107, 1249
HpaII	CCGG	6 cut(s)	1643, 2131, 2273, 2397, 2413, 2440
HphI	GGTGA	6 cut(s)	357, 647, 1216, 1540, 2001, 2045
Hpy166II	GTNNAC	8 cut(s)	155, 265, 358, 715, 1325, 1847, 1900, 2197
Hpy188III	TCNNGA	9 cut(s)	728, 1055, 1382, 1433, 1561, 1697, 1721, 2273, 2358
Hpy8I	GTNNAC	8 cut(s)	155, 265, 358, 715, 1325, 1847, 1900, 2197
Hpy99I	CGWCG	1 cut(s)	82
HpyAV	CCTTC	3 cut(s)	201, 1205, 1968
HpyCH4IV	ACGT	3 cut(s)	1386, 2112, 2322
HpyF3I	CTNAG	7 cut(s)	390, 579, 694, 1707, 1880, 1892, 1985
HpySE526I	ACGT	3 cut(s)	1386, 2112, 2322
Hsp92I	GRCGYC	1 cut(s)	2277
HspAI	GCGC	2 cut(s)	797, 1070
Kpn2I	TCCGGA	1 cut(s)	2272
KroI	GCCGGC	1 cut(s)	2439
KroNI	GCCGGC	1 cut(s)	2441
Ksp22I	TGATCA	1 cut(s)	1458
LmnI	GCTCC	5 cut(s)	1435, 1839, 2560, 2586, 2596
Lsp1109I	GCAGC	8 cut(s)	145, 416, 577, 710, 967, 1480, 1914, 2239
MaeI	CTAG	5 cut(s)	137, 339, 555, 681, 1394
MaeII	ACGT	3 cut(s)	1386, 2112, 2322
MboII	GAAGA	4 cut(s)	43, 119, 2195, 2509
MflI	RGATCY	1 cut(s)	305
MhlI	GDGCHC	4 cut(s)	360, 1125, 1902, 2124
MlsI	TGGCCA	1 cut(s)	1683
MluNI	TGGCCA	1 cut(s)	1683
MmeI	TCCRAC	2 cut(s)	515, 1123
Mox20I	TGGCCA	1 cut(s)	1683
Mph1103I	ATGCAT	3 cut(s)	1024, 2151, 2607
MroI	TCCGGA	1 cut(s)	2272
MroNI	GCCGGC	1 cut(s)	2439
MroXI	GAANNNNTTC	1 cut(s)	1295
MscI	TGGCCA	1 cut(s)	1683
MslI	CAYNNNNRTG	5 cut(s)	350, 1257, 1488, 1530, 1667
Msp20I	TGGCCA	1 cut(s)	1683
MspA1I	CMGCKG	3 cut(s)	407, 590, 1906
MspCI	CTTAAG	1 cut(s)	1091
MspI	CCGG	6 cut(s)	1643, 2131, 2273, 2397, 2413, 2440
MspR9I	CCNGG	4 cut(s)	496, 1165, 1813, 2398
Mva1269I	GAATGC	1 cut(s)	2607
MvaI	CCWGG	3 cut(s)	496, 1165, 1813
NaeI	GCCGGC	1 cut(s)	2441
NciI	CCSGG	1 cut(s)	2398
NcoI	CCATGG	3 cut(s)	177, 819, 1137
NdeI	CATATG	1 cut(s)	2014
NgoMIV	GCCGGC	1 cut(s)	2439
NheI	GCTAGC	1 cut(s)	136
NlaIV	GGNNCC	7 cut(s)	208, 500, 975, 1177, 1746, 2438, 2562
NmuCI	GTSAC	6 cut(s)	1231, 1546, 1616, 2033, 2047, 2425
NsbI	TGCGCA	1 cut(s)	798
NsiI	ATGCAT	3 cut(s)	1024, 2151, 2607
NspI	RCATGY	2 cut(s)	527, 1624
OliI	CACNNNNGTG	1 cut(s)	350
PagI	TCATGA	3 cut(s)	727, 1560, 1720
PciI	ACATGT	1 cut(s)	523
PctI	GAATGC	1 cut(s)	2607
PdiI	GCCGGC	1 cut(s)	2441
PdmI	GAANNNNTTC	1 cut(s)	1295
PfeI	GAWTC	3 cut(s)	465, 1107, 1249
PfoI	TCCNGGA	1 cut(s)	2396
PpuMI	RGGWCCY	4 cut(s)	242, 974, 1675, 2096
PscI	ACATGT	1 cut(s)	523
PshBI	ATTAAT	1 cut(s)	282
Psp5II	RGGWCCY	4 cut(s)	242, 974, 1675, 2096
Psp6I	CCWGG	3 cut(s)	494, 1163, 1811
PspEI	GGTNACC	2 cut(s)	1546, 2033
PspGI	CCWGG	3 cut(s)	494, 1163, 1811
PspN4I	GGNNCC	7 cut(s)	208, 500, 975, 1177, 1746, 2438, 2562
PspPI	GGNCC	9 cut(s)	62, 242, 498, 620, 858, 974, 1675, 2096, 2437
PspPPI	RGGWCCY	4 cut(s)	242, 974, 1675, 2096
PstI	CTGCAG	2 cut(s)	985, 1932
PstNI	CAGNNNCTG	3 cut(s)	578, 2099, 2491
PsuI	RGATCY	1 cut(s)	305
PvuII	CAGCTG	3 cut(s)	407, 590, 1906
RsaI	GTAC	1 cut(s)	2338
RsaNI	GTAC	1 cut(s)	2337
RseI	CAYNNNNRTG	5 cut(s)	350, 1257, 1488, 1530, 1667
Sau96I	GGNCC	9 cut(s)	62, 242, 498, 620, 858, 974, 1675, 2096, 2437
ScrFI	CCNGG	4 cut(s)	496, 1165, 1813, 2398
SduI	GDGCHC	4 cut(s)	360, 1125, 1902, 2124
SfcI	CTRYAG	2 cut(s)	981, 1928
SinI	GGWCC	5 cut(s)	242, 620, 974, 1675, 2096
SmiMI	CAYNNNNRTG	5 cut(s)	350, 1257, 1488, 1530, 1667
SmlI	CTYRAG	2 cut(s)	1091, 1697
SmoI	CTYRAG	2 cut(s)	1091, 1697
SpeI	ACTAGT	1 cut(s)	554
SsiI	CCGC	6 cut(s)	990, 1096, 1316, 1631, 2231, 2443
SspMI	CTAG	5 cut(s)	137, 339, 555, 681, 1394
StyD4I	CCNGG	4 cut(s)	494, 1163, 1811, 2396
StyI	CCWWGG	7 cut(s)	177, 819, 1137, 1153, 1393, 1578, 1678
TaiI	ACGT	3 cut(s)	1389, 2115, 2325
TaqI	TCGA	2 cut(s)	1060, 1148
TauI	GCSGC	2 cut(s)	992, 1099
TfiI	GAWTC	3 cut(s)	465, 1107, 1249
TscAI	CASTG	7 cut(s)	799, 928, 1456, 1691, 1888, 2487, 2527
TseFI	GTSAC	6 cut(s)	1231, 1546, 1616, 2033, 2047, 2425
TseI	GCWGC	8 cut(s)	133, 404, 590, 698, 980, 1493, 1927, 2252
Tsp45I	GTSAC	6 cut(s)	1231, 1546, 1616, 2033, 2047, 2425
TspDTI	ATGAA	9 cut(s)	81, 126, 763, 926, 1241, 1304, 1709, 1737, 1742
TspGWI	ACGGA	4 cut(s)	95, 362, 701, 1747
TspRI	CASTG	7 cut(s)	799, 928, 1456, 1691, 1888, 2487, 2527
Vha464I	CTTAAG	1 cut(s)	1091
VneI	GTGCAC	2 cut(s)	356, 1898
VpaK11BI	GGWCC	5 cut(s)	242, 620, 974, 1675, 2096
VspI	ATTAAT	1 cut(s)	282
XapI	RAATTY	4 cut(s)	88, 412, 1038, 2069
XceI	RCATGY	2 cut(s)	527, 1624
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1370
XmaJI	CCTAGG	1 cut(s)	1393
XmiI	GTMKAC	2 cut(s)	154, 714
XmnI	GAANNNNTTC	1 cut(s)	1295
XspI	CTAG	5 cut(s)	137, 339, 555, 681, 1394
Zsp2I	ATGCAT	3 cut(s)	1024, 2151, 2607

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.