Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0005339

Physical Location & Seq

Genomic Coordinates Reverse (-)

28298926 .. 28303473

Gene Structure 4548 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1192100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2589 bp

ATGTTGCGAGCAAAGCAATTAAGTAACCTTTCAAGTAGTGCGAGGTCATTCTTCCTTACCGGGCCACGATGTAGTGCCACAGATGGAAATTCATGCTCTTGCTCTGAAGATGAAACTTGTGTTTCGCAAAGGCAGCTAGCACTTGCCCAAAATCCATCGACCATGGTATCCAAACCTTCAGCAAGGGCAGGAAGCATCCTTTTGGGAGATGCAGTAAAAGTGGCAGGTCTTCGTAAAGCCGAAAGTGTTGACCATACAGCTTCTATTAAAAAGGTTGCAACGGCACCACGATCTTTGGGGAGATCAGAGACTGTAAGTTATGCTAGTACCATGGATGTGGTGCACTCATCACCTCTCGTTGCAGACCAGTTTGCTAAGGCAGGAGTTGCTGCTGTAAATTTTCTGTCTGATATAGTAAATGGCAGGCTTCCTTTATCTGATGGGCTTGGAATCCTTAATAACTGTATGGTTGATCCGACCAGACCCCTCAGTGGTATCAAGCCATCACACGTGAAACAAATAAAAAAAGAGCATTTCACTAGTGTACATCCAAAACAGTCTACTGAGGTATCAGCTGCATCAAAGCCTACAAGTAACAATCATGGTCAAAAGGGCAAAGGTGAGAAATCCAGTTTCGTTAAAGGTCTAAATCATGTTCCATATTCTAGGACAGAAAGCTCAGCGGTACCTCATGTTGTATATTCGGACCGTCATGCGAACAGGTCTATGCCACCGAAATCAAAACCTCATTCAAACTTCATTCCAAATTACAGTTCCAGTGTGCAGGCTTCTGATGCAGAGACCACGGGTCGTGCCTCTAAAAGTTTCAACAGGCCCATAAGAGATGTGAAAATGCCAACAGGAATAGCTCCCCAAATCAACAGACAGTATGCTCACACTGGGAATGTTGTTCATAATGTTTCTCACATACTGCAACAGATGAGATGGGGTCCTGCTGCAGTGGCCGCTCTTGGAAATCTCAACTGTTCAATGGATGCATATCACGCAAACCAAATTTTAAAGCAACTTCAAGATCATTCCGTTGCTCTTGGATTTTTTTATTGGCTTAAGCGGCAAGCAGGATTCAAGCATGATGGGCACACTTATACCACCATGGTTGGGATCCTTGGCCGTTCCAGGCAGTTTGGTGCCATTAACAAATTGCTTAATCAGATGGTGAAAGAAGGTTGCCAGCCAAATGTTGTGACATATAACCGTCTAATTCATAGTTATGGCCGTGCAAACTATTTAAAAGAAGCAATGAATGTGTTTAATCAAATGCAAGAAGCAGGATGTGAACCTGACCGTGTCACCTACTGCACACTAATTGACATCCATGCAAAAGCTGGTTTTCTTGATGTTGCCCTAGGATTGTATGACAGGATGCAAGAGGCTGGTCTTGCTCCTGACACATTCACGTACAGTGTTATGATCAACTGTCTCGGGAAAGCTGGTCATTTAGCTGCTGCTCATCGGCTATTTTGTGAGATGGTTAATCATGGTTGTGTTCCCAATTTGGTCACGTATAATATCATGATAGCTTTGCAGGCCAAAGCAAGGAATTATGAAACTTCTTTAAAGCTCTATCGTGACATGCAGGGTGCGGGATTTGAACCAGATAAAGTAACTTATAGCATAATAATGGAGGTCCTTGGCCATTGTGGATATCTTGAGGAAGCCGAGGCGATTTTTCATGAAATGAAACGGAAGAACTGGGTACCTGATGAGCCTGTTTATGGTCTTTTGGTGGATTTGTGGGGTAAGGCTGGTAATGTGGAAAAAGCCTGGAATTGGTATCAAGCAATGCTTCATGCAGGTTTACGTCCTAATGTGCCTACTTGCAATTCTCTGCTCAGTGCGTTTCTTAGGGTGCACCAGCTGTCAGATGCCTATCACTTGCTGCAGAGCATGATGGGTTTAGGTCTAAACCCATCTCTGCAGACATATACCTTGCTTATCAGTTGTTGCACAGAAGCACAGTCACCATATGACATGAGATTTTGCGGTGAGCTTATGGCAGTCACAGGCCATCCTGCACATACATTCTTACTGTCTATGCCATCGGCAGGACCAGATGGTCAAAACGTGCGAGATCACATGTGCAGGTTCTTGGATCTGATGCATAGCGAGGATAGAGAAAGCAAGAGGGGACTAGTAGATGCGGTAGTGGATTTTCTTCACAAGGCGGGGCTAAAGGAGGAGGCGGGTTCTGTCTGGGAGGTGGCTGCACAAAAGAATGTCTATCCAGATGCCATCACAGAGAAAAGCTCATGCTATTGGCTTATAAACCTGCACGTTATGTCCGACGGTACTGCTGTCACAGCACTATCAAGAACACTTGCTTGGTTTCGCCAACAAATGCTAATATCCGGGATTTGTCCAAGCCGGATTGATATTGTTACAGGATGGGGCCGGCGGAGTAGGGTCACAGGATCTTCTTTGGTTAGGCAAGCAGTGCAGGAACTGCTGAATGTTTTTCGCTTCCCGTTCTTCACTGAAAACGGCAATTCAGGTTGTTTTATAGGATGTGGGGAGCCTCTTAACAGGTGGTTGCTCCAATCTTATGTGGAGCGAATGCATTTACTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

863

Amino Acids

95.07

Weight (kDa)

8.91

Isoelectric Point (pI)

40.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_long	PF17177	360 - 450	5.2e-06	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	360 - 412	2.9e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	367 - 409	1.6e-09	PPR repeat family
PPR_3	PF13812	387 - 445	3.5e-13	Pentatricopeptide repeat domain
PPR_1	PF12854	396 - 428	2.8e-11	PPR repeat
PPR_2	PF13041	399 - 447	1.1e-16	PPR repeat family
PPR	PF01535	402 - 432	9e-08	PPR repeat
PPR_3	PF13812	423 - 483	8.9e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	431 - 463	3.1e-07	PPR repeat
PPR	PF01535	437 - 467	7.2e-06	PPR repeat
PPR_2	PF13041	437 - 483	6.1e-11	PPR repeat family
PPR_long	PF17177	455 - 551	1.6e-06	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	459 - 515	4.8e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	466 - 498	3.8e-08	PPR repeat
PPR	PF01535	472 - 502	2.4e-06	PPR repeat
PPR_2	PF13041	477 - 513	6.1e-11	PPR repeat family
PPR_2	PF13041	504 - 551	7.3e-12	PPR repeat family
PPR_1	PF12854	536 - 568	6.5e-06	PPR repeat
PPR	PF01535	542 - 571	6.2e-06	PPR repeat
PPR_long	PF17177	558 - 659	6e-06	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	574 - 621	2.5e-06	PPR repeat family
PPR_3	PF13812	602 - 656	2.4e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	609 - 656	5.7e-09	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0005339)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18900 AT1G18900 AT1G18900 AT1G18900
fragaria_vesca	FvH4_5g15730 FvH4_5g15730 FvH4_5g15730
malus_domestica	MD06G1185900.v1.1 MD14G1192100.v1.1
prunus_persica	Prupe.5G184700_v2.0.a1 Prupe.5G184700_v2.0.a1 Prupe.5G184700_v2.0.a1 Prupe.5G184700_v2.0.a1 Prupe.5G184700_v2.0.a1
pyrus_communis	pycom06g16530 pycom14g15980
rosa_chinensis	RchiOBHm_Chr7g0180381
rosa_laevigata	RLG00000005272
rosa_multiflora	Rmu_sc0003526.1_g000009
rosa_roxburghii	Rroxscaffold_3G00272940
rosa_rugosa	Rorug06G0432600 Rorug06G0432700 Rorug06G0432800 Rorug06G0432800
rosa_samantha	Rh7AG033200 Rh7BG033100 Rh7CG035000 Rh7DG033600
rosa_wichuraiana	Rw7G002770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2285
AasI	GACNNNNNNGTC	1 cut(s)	2076
Acc36I	ACCTGC	4 cut(s)	215, 1805, 2094, 2298
Acc65I	GGTACC	2 cut(s)	685, 1717
AccB1I	GGYRCC	4 cut(s)	283, 685, 1148, 1717
AccBSI	CCGCTC	1 cut(s)	968
AccI	GTMKAC	1 cut(s)	560
AciI	CCGC	9 cut(s)	683, 966, 1072, 1604, 2004, 2162, 2186, 2204, 2416
AclWI	GGATC	5 cut(s)	467, 1117, 1130, 2121, 2440
AcoI	YGGCCR	4 cut(s)	963, 1129, 1234, 1654
AcsI	RAATTY	3 cut(s)	88, 397, 1014
AcuI	CTGAAG	2 cut(s)	126, 162
AcvI	CACGTG	1 cut(s)	511
AfaI	GTAC	6 cut(s)	328, 546, 687, 1421, 1719, 2311
AfiI	CCNNNNNNNGG	6 cut(s)	491, 1119, 1557, 1736, 1791, 2066
AflII	CTTAAG	1 cut(s)	1067
AflIII	ACRYGT	2 cut(s)	508, 2097
AgsI	TTSAA	7 cut(s)	33, 753, 829, 990, 1031, 1087, 1613
AhdI	GACNNNNNGTC	1 cut(s)	807
AhlI	ACTAGT	2 cut(s)	539, 2152
AjnI	CCWGG	2 cut(s)	1136, 1784
AjuI	GAANNNNNNNTTGG	4 cut(s)	757, 789, 2326, 2358
Alw21I	GWGCWC	2 cut(s)	345, 1875
Alw26I	GTCTC	3 cut(s)	302, 794, 1445
Alw44I	GTGCAC	2 cut(s)	341, 1871
AlwI	GGATC	5 cut(s)	467, 1117, 1130, 2121, 2440
AlwNI	CAGNNNCTG	2 cut(s)	311, 2464
Ama87I	CYCGRG	1 cut(s)	1442
AoxI	GGCC	9 cut(s)	62, 833, 963, 1129, 1234, 1548, 1654, 2026, 2410
ApaLI	GTGCAC	2 cut(s)	341, 1871
ApeKI	GCWGC	8 cut(s)	133, 389, 575, 956, 1463, 1466, 1900, 2225
ApoI	RAATTY	3 cut(s)	88, 397, 1014
Asp718I	GGTACC	2 cut(s)	685, 1717
AspA2I	CCTAGG	1 cut(s)	1366
AspS9I	GGNCC	7 cut(s)	62, 706, 834, 950, 1648, 2069, 2410
AsuC2I	CCSGG	2 cut(s)	61, 2371
AsuHPI	GGTGA	6 cut(s)	342, 632, 1189, 1303, 1974, 2018
AsuNHI	GCTAGC	1 cut(s)	136
AvaI	CYCGRG	1 cut(s)	1442
AvaII	GGWCC	4 cut(s)	706, 950, 1648, 2069
AvrII	CCTAGG	1 cut(s)	1366
BaeGI	GKGCMC	3 cut(s)	345, 1101, 1875
BaeI	ACNNNNGTAYC	6 cut(s)	150, 183, 552, 585, 2301, 2334
BalI	TGGCCA	1 cut(s)	1656
BamHI	GGATCC	1 cut(s)	1122
BanI	GGYRCC	4 cut(s)	283, 685, 1148, 1717
BbrPI	CACGTG	1 cut(s)	511
BbsI	GAAGAC	1 cut(s)	221
Bbv12I	GWGCWC	2 cut(s)	345, 1875
BbvI	GCAGC	8 cut(s)	145, 376, 562, 943, 1450, 1453, 1887, 2212
BceAI	ACGGC	4 cut(s)	297, 1116, 1221, 2518
BcgI	CGANNNNNNTGC	2 cut(s)	2294, 2328
BciT130I	CCWGG	2 cut(s)	1138, 1786
BciVI	GTATCC	1 cut(s)	178
BclI	TGATCA	1 cut(s)	1431
BcnI	CCSGG	2 cut(s)	61, 2371
BcoDI	GTCTC	3 cut(s)	302, 794, 1445
BcuI	ACTAGT	2 cut(s)	539, 2152
BfaI	CTAG	6 cut(s)	137, 324, 540, 666, 1367, 2153
BfmI	CTRYAG	4 cut(s)	957, 1901, 1937, 2585
BfrI	CTTAAG	1 cut(s)	1067
BfuAI	ACCTGC	4 cut(s)	215, 1805, 2094, 2298
BfuI	GTATCC	1 cut(s)	178
BlnI	CCTAGG	1 cut(s)	1366
BlpI	GCTNAGC	1 cut(s)	679
Bme1390I	CCNGG	4 cut(s)	61, 1138, 1786, 2371
Bme18I	GGWCC	4 cut(s)	706, 950, 1648, 2069
BmeRI	GACNNNNNGTC	1 cut(s)	807
BmeT110I	CYCGRG	1 cut(s)	1442
BmgT120I	GGNCC	7 cut(s)	62, 706, 834, 950, 1648, 2069, 2410
BmiI	GGNNCC	8 cut(s)	285, 687, 951, 1124, 1150, 1719, 2411, 2535
BmrFI	CCNGG	4 cut(s)	61, 1138, 1786, 2371
BmrI	ACTGGG	2 cut(s)	909, 1723
BmtI	GCTAGC	1 cut(s)	140
BmuI	ACTGGG	2 cut(s)	909, 1723
BpiI	GAAGAC	1 cut(s)	221
BplI	GAGNNNNNCTC	2 cut(s)	2252, 2284
Bpu10I	CCTNAGC	1 cut(s)	375
Bpu1102I	GCTNAGC	1 cut(s)	679
BpuEI	CTTGAG	1 cut(s)	1691
BpuMI	CCSGG	2 cut(s)	61, 2371
BsaAI	YACGTR	3 cut(s)	511, 1419, 1524
BsaBI	GATNNNNATC	2 cut(s)	999, 1890
BsaI	GGTCTC	1 cut(s)	794
BsaJI	CCNNGG	8 cut(s)	162, 330, 804, 1113, 1126, 1366, 1651, 1680
BsaXI	ACNNNNNCTCC	2 cut(s)	198, 228
Bsc4I	CCNNNNNNNGG	6 cut(s)	491, 1119, 1557, 1736, 1791, 2066
Bse118I	RCCGGY	1 cut(s)	2412
Bse1I	ACTGG	5 cut(s)	367, 630, 777, 904, 1718
Bse3DI	GCAATG	2 cut(s)	1266, 1809
Bse8I	GATNNNNATC	2 cut(s)	999, 1890
BseBI	CCWGG	2 cut(s)	1138, 1786
BseDI	CCNNGG	8 cut(s)	162, 330, 804, 1113, 1126, 1366, 1651, 1680
BseJI	GATNNNNATC	2 cut(s)	999, 1890
BseLI	CCNNNNNNNGG	6 cut(s)	491, 1119, 1557, 1736, 1791, 2066
BseMI	GCAATG	2 cut(s)	1266, 1809
BseMII	CTCAG	4 cut(s)	502, 555, 693, 1867
BseNI	ACTGG	5 cut(s)	367, 630, 777, 904, 1718
BseRI	GAGGAG	1 cut(s)	2213
BseSI	GKGCMC	3 cut(s)	345, 1101, 1875
BseXI	GCAGC	8 cut(s)	145, 376, 562, 943, 1450, 1453, 1887, 2212
BsgI	GTGCAG	7 cut(s)	803, 1303, 2019, 2122, 2211, 2276, 2477
BshFI	GGCC	9 cut(s)	64, 835, 965, 1131, 1236, 1550, 1656, 2028, 2412
BshNI	GGYRCC	4 cut(s)	283, 685, 1148, 1717
BsiHKAI	GWGCWC	2 cut(s)	345, 1875
BsiHKCI	CYCGRG	1 cut(s)	1442
BsiSI	CCGG	4 cut(s)	60, 2370, 2386, 2413
BslFI	GGGAC	1 cut(s)	2163
BslI	CCNNNNNNNGG	6 cut(s)	491, 1119, 1557, 1736, 1791, 2066
BsmAI	GTCTC	3 cut(s)	302, 794, 1445
BsmFI	GGGAC	1 cut(s)	2163
BsmI	GAATGC	1 cut(s)	2580
BsnI	GGCC	9 cut(s)	64, 835, 965, 1131, 1236, 1550, 1656, 2028, 2412
Bso31I	GGTCTC	1 cut(s)	794
BsoBI	CYCGRG	1 cut(s)	1442
Bsp1286I	GDGCHC	3 cut(s)	345, 1101, 1875
Bsp1407I	TGTACA	1 cut(s)	544
Bsp143I	GATC	9 cut(s)	290, 302, 472, 1033, 1122, 1431, 2092, 2113, 2432
Bsp1720I	GCTNAGC	1 cut(s)	679
Bsp19I	CCATGG	3 cut(s)	162, 330, 1113
BspACI	CCGC	9 cut(s)	683, 966, 1072, 1604, 2004, 2162, 2186, 2204, 2416
BspANI	GGCC	9 cut(s)	64, 835, 965, 1131, 1236, 1550, 1656, 2028, 2412
BspCNI	CTCAG	4 cut(s)	501, 556, 692, 1866
BspHI	TCATGA	2 cut(s)	1533, 1693
BspLI	GGNNCC	8 cut(s)	285, 687, 951, 1124, 1150, 1719, 2411, 2535
BspMAI	CTGCAG	3 cut(s)	961, 1905, 1941
BspMI	ACCTGC	4 cut(s)	215, 1805, 2094, 2298
BspOI	GCTAGC	1 cut(s)	140
BspPI	GGATC	5 cut(s)	467, 1117, 1130, 2121, 2440
BspT107I	GGYRCC	4 cut(s)	283, 685, 1148, 1717
BspTI	CTTAAG	1 cut(s)	1067
BspTNI	GGTCTC	1 cut(s)	794
BsrBI	CCGCTC	1 cut(s)	968
BsrDI	GCAATG	2 cut(s)	1266, 1809
BsrFI	RCCGGY	1 cut(s)	2412
BsrGI	TGTACA	1 cut(s)	544
BsrI	ACTGG	5 cut(s)	367, 630, 777, 904, 1718
BssAI	RCCGGY	1 cut(s)	2412
BssECI	CCNNGG	8 cut(s)	162, 330, 804, 1113, 1126, 1366, 1651, 1680
BssMI	GATC	9 cut(s)	290, 302, 472, 1033, 1122, 1431, 2092, 2113, 2432
BssT1I	CCWWGG	6 cut(s)	162, 330, 1113, 1126, 1366, 1651
Bst2UI	CCWGG	2 cut(s)	1138, 1786
BstAFI	CTTAAG	1 cut(s)	1067
BstAPI	GCANNNNNTGC	3 cut(s)	386, 2455, 2464
BstAUI	TGTACA	1 cut(s)	544
BstBAI	YACGTR	3 cut(s)	511, 1419, 1524
BstC8I	GCNNGC	9 cut(s)	9, 138, 425, 786, 1077, 1193, 1548, 2414, 2451
BstDEI	CTNAG	6 cut(s)	375, 488, 564, 679, 1853, 1865
BstDSI	CCRYGG	4 cut(s)	162, 330, 804, 1113
BstKTI	GATC	9 cut(s)	293, 305, 475, 1036, 1125, 1434, 2095, 2116, 2435
BstMAI	GTCTC	3 cut(s)	302, 794, 1445
BstMBI	GATC	9 cut(s)	290, 302, 472, 1033, 1122, 1431, 2092, 2113, 2432
BstNI	CCWGG	2 cut(s)	1138, 1786
BstNSI	RCATGY	2 cut(s)	1597, 2101
BstSCI	CCNGG	4 cut(s)	59, 1136, 1784, 2369
BstSFI	CTRYAG	4 cut(s)	957, 1901, 1937, 2585
BstSLI	GKGCMC	3 cut(s)	345, 1101, 1875
BstV1I	GCAGC	8 cut(s)	145, 376, 562, 943, 1450, 1453, 1887, 2212
BstV2I	GAAGAC	1 cut(s)	221
BstX2I	RGATCY	3 cut(s)	1122, 2113, 2432
BstXI	CCANNNNNNTGG	1 cut(s)	337
BstYI	RGATCY	3 cut(s)	1122, 2113, 2432
BsuI	GTATCC	1 cut(s)	178
BsuRI	GGCC	9 cut(s)	64, 835, 965, 1131, 1236, 1550, 1656, 2028, 2412
BtgI	CCRYGG	4 cut(s)	162, 330, 804, 1113
BtsI	GCAGTG	2 cut(s)	966, 2460
BtsIMutI	CAGTG	8 cut(s)	496, 784, 897, 966, 1429, 1861, 2460, 2493
BveI	ACCTGC	4 cut(s)	215, 1805, 2094, 2298
Cac8I	GCNNGC	9 cut(s)	9, 138, 425, 786, 1077, 1193, 1548, 2414, 2451
CaiI	CAGNNNCTG	2 cut(s)	311, 2464
CciI	TCATGA	2 cut(s)	1533, 1693
Cfr10I	RCCGGY	1 cut(s)	2412
Cfr13I	GGNCC	7 cut(s)	62, 706, 834, 950, 1648, 2069, 2410
CpoI	CGGWCCG	1 cut(s)	706
Csp6I	GTAC	6 cut(s)	327, 545, 686, 1420, 1718, 2310
CspCI	CAANNNNNGTGG	4 cut(s)	276, 311, 1099, 1134
CspI	CGGWCCG	1 cut(s)	706
CviQI	GTAC	6 cut(s)	327, 545, 686, 1420, 1718, 2310
DdeI	CTNAG	6 cut(s)	375, 488, 564, 679, 1853, 1865
DpnI	GATC	9 cut(s)	292, 304, 474, 1035, 1124, 1433, 2094, 2115, 2434
DpnII	GATC	9 cut(s)	290, 302, 472, 1033, 1122, 1431, 2092, 2113, 2432
DraI	TTTAAA	3 cut(s)	1020, 1251, 1578
DrdI	GACNNNNNNGTC	1 cut(s)	2076
DriI	GACNNNNNGTC	1 cut(s)	807
DseDI	GACNNNNNNGTC	1 cut(s)	2076
EaeI	YGGCCR	4 cut(s)	963, 1129, 1234, 1654
Eam1105I	GACNNNNNGTC	1 cut(s)	807
EciI	GGCGGA	1 cut(s)	2431
Eco130I	CCWWGG	6 cut(s)	162, 330, 1113, 1126, 1366, 1651
Eco31I	GGTCTC	1 cut(s)	794
Eco32I	GATATC	1 cut(s)	1667
Eco47I	GGWCC	4 cut(s)	706, 950, 1648, 2069
Eco57I	CTGAAG	2 cut(s)	126, 162
Eco72I	CACGTG	1 cut(s)	511
Eco88I	CYCGRG	1 cut(s)	1442
EcoO109I	RGGNCCY	2 cut(s)	950, 1648
EcoRII	CCWGG	2 cut(s)	1136, 1784
EcoRV	GATATC	1 cut(s)	1667
EcoT14I	CCWWGG	6 cut(s)	162, 330, 1113, 1126, 1366, 1651
EcoT22I	ATGCAT	3 cut(s)	1000, 2124, 2580
ErhI	CCWWGG	6 cut(s)	162, 330, 1113, 1126, 1366, 1651
FalI	AAGNNNNNCTT	2 cut(s)	1791, 1823
FaqI	GGGAC	1 cut(s)	2163
FauI	CCCGC	3 cut(s)	1597, 2179, 2197
FauNDI	CATATG	1 cut(s)	1987
FbaI	TGATCA	1 cut(s)	1431
FblI	GTMKAC	1 cut(s)	560
FspBI	CTAG	6 cut(s)	137, 324, 540, 666, 1367, 2153
HaeIII	GGCC	9 cut(s)	64, 835, 965, 1131, 1236, 1550, 1656, 2028, 2412
HapII	CCGG	4 cut(s)	60, 2370, 2386, 2413
HincII	GTYRAC	1 cut(s)	250
HindII	GTYRAC	1 cut(s)	250
HinfI	GANTC	2 cut(s)	450, 1083
HpaII	CCGG	4 cut(s)	60, 2370, 2386, 2413
HphI	GGTGA	6 cut(s)	342, 632, 1189, 1303, 1974, 2018
Hpy166II	GTNNAC	7 cut(s)	250, 343, 545, 561, 1298, 1820, 1873
Hpy8I	GTNNAC	7 cut(s)	250, 343, 545, 561, 1298, 1820, 1873
Hpy99I	CGWCG	1 cut(s)	2309
HpyAV	CCTTC	2 cut(s)	186, 1178
HpyCH4IV	ACGT	6 cut(s)	510, 1418, 1523, 1822, 2085, 2295
HpyF3I	CTNAG	6 cut(s)	375, 488, 564, 679, 1853, 1865
HpySE526I	ACGT	6 cut(s)	510, 1418, 1523, 1822, 2085, 2295
KpnI	GGTACC	2 cut(s)	689, 1721
KroI	GCCGGC	1 cut(s)	2412
KroNI	GCCGGC	1 cut(s)	2414
Ksp22I	TGATCA	1 cut(s)	1431
Kzo9I	GATC	9 cut(s)	290, 302, 472, 1033, 1122, 1431, 2092, 2113, 2432
LmnI	GCTCC	5 cut(s)	874, 1408, 2533, 2559, 2569
Lsp1109I	GCAGC	8 cut(s)	145, 376, 562, 943, 1450, 1453, 1887, 2212
MaeI	CTAG	6 cut(s)	137, 324, 540, 666, 1367, 2153
MaeII	ACGT	6 cut(s)	510, 1418, 1523, 1822, 2085, 2295
MalI	GATC	9 cut(s)	292, 304, 474, 1035, 1124, 1433, 2094, 2115, 2434
MbiI	CCGCTC	1 cut(s)	968
MboI	GATC	9 cut(s)	290, 302, 472, 1033, 1122, 1431, 2092, 2113, 2432
MboII	GAAGA	7 cut(s)	43, 119, 221, 1720, 2168, 2427, 2482
MflI	RGATCY	3 cut(s)	1122, 2113, 2432
MhlI	GDGCHC	3 cut(s)	345, 1101, 1875
MlsI	TGGCCA	1 cut(s)	1656
MluNI	TGGCCA	1 cut(s)	1656
MmeI	TCCRAC	2 cut(s)	500, 2328
Mox20I	TGGCCA	1 cut(s)	1656
Mph1103I	ATGCAT	3 cut(s)	1000, 2124, 2580
MroNI	GCCGGC	1 cut(s)	2412
MscI	TGGCCA	1 cut(s)	1656
MslI	CAYNNNNRTG	4 cut(s)	335, 1230, 1503, 1640
Msp20I	TGGCCA	1 cut(s)	1656
MspA1I	CMGCKG	3 cut(s)	575, 683, 1879
MspCI	CTTAAG	1 cut(s)	1067
MspI	CCGG	4 cut(s)	60, 2370, 2386, 2413
MspR9I	CCNGG	4 cut(s)	61, 1138, 1786, 2371
Mva1269I	GAATGC	1 cut(s)	2580
MvaI	CCWGG	2 cut(s)	1138, 1786
NaeI	GCCGGC	1 cut(s)	2414
NciI	CCSGG	2 cut(s)	61, 2371
NcoI	CCATGG	3 cut(s)	162, 330, 1113
NdeI	CATATG	1 cut(s)	1987
NdeII	GATC	9 cut(s)	290, 302, 472, 1033, 1122, 1431, 2092, 2113, 2432
NgoMIV	GCCGGC	1 cut(s)	2412
NheI	GCTAGC	1 cut(s)	136
NlaIV	GGNNCC	8 cut(s)	285, 687, 951, 1124, 1150, 1719, 2411, 2535
NmeAIII	GCCGAG	1 cut(s)	1705
NmuCI	GTSAC	8 cut(s)	1204, 1309, 1519, 1589, 1980, 2020, 2317, 2425
NsiI	ATGCAT	3 cut(s)	1000, 2124, 2580
NspI	RCATGY	2 cut(s)	1597, 2101
PagI	TCATGA	2 cut(s)	1533, 1693
PciI	ACATGT	1 cut(s)	2097
PcsI	WCGNNNNNNNCGW	1 cut(s)	2301
PctI	GAATGC	1 cut(s)	2580
PdiI	GCCGGC	1 cut(s)	2414
PfeI	GAWTC	2 cut(s)	450, 1083
PflFI	GACNNNGTC	1 cut(s)	1307
PfoI	TCCNGGA	1 cut(s)	2369
PmaCI	CACGTG	1 cut(s)	511
PmlI	CACGTG	1 cut(s)	511
Ppu21I	YACGTR	3 cut(s)	511, 1419, 1524
PpuMI	RGGWCCY	2 cut(s)	950, 1648
PscI	ACATGT	1 cut(s)	2097
PsiI	TTATAA	1 cut(s)	2285
Psp5II	RGGWCCY	2 cut(s)	950, 1648
Psp6I	CCWGG	2 cut(s)	1136, 1784
PspCI	CACGTG	1 cut(s)	511
PspGI	CCWGG	2 cut(s)	1136, 1784
PspN4I	GGNNCC	8 cut(s)	285, 687, 951, 1124, 1150, 1719, 2411, 2535
PspPI	GGNCC	7 cut(s)	62, 706, 834, 950, 1648, 2069, 2410
PspPPI	RGGWCCY	2 cut(s)	950, 1648
PstI	CTGCAG	3 cut(s)	961, 1905, 1941
PstNI	CAGNNNCTG	2 cut(s)	311, 2464
PsuI	RGATCY	3 cut(s)	1122, 2113, 2432
PsyI	GACNNNGTC	1 cut(s)	1307
PvuII	CAGCTG	2 cut(s)	575, 1879
RsaI	GTAC	6 cut(s)	328, 546, 687, 1421, 1719, 2311
RsaNI	GTAC	6 cut(s)	327, 545, 686, 1420, 1718, 2310
RseI	CAYNNNNRTG	4 cut(s)	335, 1230, 1503, 1640
Rsr2I	CGGWCCG	1 cut(s)	706
RsrII	CGGWCCG	1 cut(s)	706
Sau3AI	GATC	9 cut(s)	290, 302, 472, 1033, 1122, 1431, 2092, 2113, 2432
Sau96I	GGNCC	7 cut(s)	62, 706, 834, 950, 1648, 2069, 2410
ScrFI	CCNGG	4 cut(s)	61, 1138, 1786, 2371
SduI	GDGCHC	3 cut(s)	345, 1101, 1875
SfcI	CTRYAG	4 cut(s)	957, 1901, 1937, 2585
SinI	GGWCC	4 cut(s)	706, 950, 1648, 2069
SmiMI	CAYNNNNRTG	4 cut(s)	335, 1230, 1503, 1640
SmlI	CTYRAG	2 cut(s)	1067, 1670
SmoI	CTYRAG	2 cut(s)	1067, 1670
SpeI	ACTAGT	2 cut(s)	539, 2152
SsiI	CCGC	9 cut(s)	683, 966, 1072, 1604, 2004, 2162, 2186, 2204, 2416
SspMI	CTAG	6 cut(s)	137, 324, 540, 666, 1367, 2153
StyD4I	CCNGG	4 cut(s)	59, 1136, 1784, 2369
StyI	CCWWGG	6 cut(s)	162, 330, 1113, 1126, 1366, 1651
TaiI	ACGT	6 cut(s)	513, 1421, 1526, 1825, 2088, 2298
TaqI	TCGA	1 cut(s)	158
TatI	WGTACW	1 cut(s)	544
TauI	GCSGC	2 cut(s)	968, 1075
TfiI	GAWTC	2 cut(s)	450, 1083
TscAI	CASTG	8 cut(s)	496, 784, 904, 966, 1429, 1861, 2460, 2500
TseFI	GTSAC	8 cut(s)	1204, 1309, 1519, 1589, 1980, 2020, 2317, 2425
TseI	GCWGC	8 cut(s)	133, 389, 575, 956, 1463, 1466, 1900, 2225
Tsp45I	GTSAC	8 cut(s)	1204, 1309, 1519, 1589, 1980, 2020, 2317, 2425
TspGWI	ACGGA	2 cut(s)	1030, 1720
TspRI	CASTG	8 cut(s)	496, 784, 904, 966, 1429, 1861, 2460, 2500
Tth111I	GACNNNGTC	1 cut(s)	1307
Vha464I	CTTAAG	1 cut(s)	1067
VneI	GTGCAC	2 cut(s)	341, 1871
VpaK11BI	GGWCC	4 cut(s)	706, 950, 1648, 2069
XapI	RAATTY	3 cut(s)	88, 397, 1014
XceI	RCATGY	2 cut(s)	1597, 2101
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1343
XmaJI	CCTAGG	1 cut(s)	1366
XmiI	GTMKAC	1 cut(s)	560
XspI	CTAG	6 cut(s)	137, 324, 540, 666, 1367, 2153
Zsp2I	ATGCAT	3 cut(s)	1000, 2124, 2580

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.