TPLATE-like
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT3G01780 AT3G01780 |
| fragaria_vesca | FvH4_5g15940 |
| malus_domestica | MD06G1184100.v1.1 MD14G1190000.v1.1 |
| prunus_persica | Prupe.5G183000_v2.0.a1 |
| pyrus_communis | pycom06g16320 pycom06g16330 pycom14g15780 |
| rosa_chinensis | RchiOBHm_Chr7g0180111 |
| rosa_laevigata | RLG00000005292 |
| rosa_multiflora | Rmu_sc0017616.1_g000007 Rmu_ssc0000119.1_g000034 |
| rosa_roxburghii | Rroxscaffold_3G00273120 |
| rosa_rugosa | Rorug06G0430700 |
| rosa_samantha | Rh7AG031100 Rh7BG031000 Rh7CG033000 Rh7DG031600 |
| rosa_wichuraiana | Rw7G002580 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 2073 |
| AasI | GACNNNNNNGTC | 2 cut(s) | 113, 275 |
| AatII | GACGTC | 1 cut(s) | 279 |
| Acc16I | TGCGCA | 1 cut(s) | 1850 |
| Acc36I | ACCTGC | 2 cut(s) | 1114, 2073 |
| AccB1I | GGYRCC | 1 cut(s) | 907 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1936 |
| AccBSI | CCGCTC | 2 cut(s) | 39, 198 |
| AccI | GTMKAC | 5 cut(s) | 1525, 1714, 2270, 3045, 3470 |
| AccII | CGCG | 5 cut(s) | 105, 308, 647, 1193, 1749 |
| AccIII | TCCGGA | 1 cut(s) | 1251 |
| AcoI | YGGCCR | 6 cut(s) | 100, 508, 1272, 1552, 2087, 2783 |
| AcsI | RAATTY | 7 cut(s) | 637, 739, 1662, 1870, 2721, 2768, 3080 |
| AcuI | CTGAAG | 4 cut(s) | 1863, 2595, 2655, 3204 |
| AcvI | CACGTG | 1 cut(s) | 434 |
| AcyI | GRCGYC | 4 cut(s) | 48, 243, 276, 534 |
| AfaI | GTAC | 5 cut(s) | 971, 2520, 2810, 2818, 3176 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 222, 481, 495, 1274, 1353, 1936, 2597, 2684, 3213 |
| AflIII | ACRYGT | 2 cut(s) | 963, 1191 |
| AhdI | GACNNNNNGTC | 2 cut(s) | 379, 1532 |
| AjnI | CCWGG | 2 cut(s) | 985, 2089 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 536, 568 |
| Alw21I | GWGCWC | 6 cut(s) | 1483, 1702, 1747, 1773, 3442, 3459 |
| Alw26I | GTCTC | 9 cut(s) | 298, 404, 490, 1404, 1667, 2475, 2548, 2650, 3053 |
| Alw44I | GTGCAC | 2 cut(s) | 1479, 1698 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1499, 1969 |
| Aor13HI | TCCGGA | 1 cut(s) | 1251 |
| ApaI | GGGCCC | 1 cut(s) | 502 |
| ApaLI | GTGCAC | 2 cut(s) | 1479, 1698 |
| ApoI | RAATTY | 7 cut(s) | 637, 739, 1662, 1870, 2721, 2768, 3080 |
| ArsI | GACNNNNNNTTYG | 3 cut(s) | 28, 129, 161 |
| AspLEI | GCGC | 4 cut(s) | 68, 284, 1851, 2564 |
| AspS9I | GGNCC | 4 cut(s) | 498, 499, 747, 3392 |
| AsuNHI | GCTAGC | 1 cut(s) | 3023 |
| AvaII | GGWCC | 1 cut(s) | 3392 |
| AxyI | CCTNAGG | 1 cut(s) | 1815 |
| BaeGI | GKGCMC | 4 cut(s) | 502, 1483, 1702, 1930 |
| BalI | TGGCCA | 2 cut(s) | 2089, 2785 |
| BamHI | GGATCC | 5 cut(s) | 931, 1792, 1819, 2512, 3125 |
| BanI | GGYRCC | 1 cut(s) | 907 |
| BanII | GRGCYC | 2 cut(s) | 502, 3274 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 2981, 3013 |
| BauI | CACGAG | 1 cut(s) | 443 |
| BbrPI | CACGTG | 1 cut(s) | 434 |
| BbsI | GAAGAC | 3 cut(s) | 1001, 3312, 3381 |
| Bbv12I | GWGCWC | 6 cut(s) | 1483, 1702, 1747, 1773, 3442, 3459 |
| BceAI | ACGGC | 8 cut(s) | 116, 149, 275, 364, 521, 895, 2019, 3394 |
| BcgI | CGANNNNNNTGC | 6 cut(s) | 2144, 2178, 3067, 3101, 3419, 3453 |
| BciT130I | CCWGG | 2 cut(s) | 987, 2091 |
| BcoDI | GTCTC | 9 cut(s) | 298, 404, 490, 1404, 1667, 2475, 2548, 2650, 3053 |
| BfaI | CTAG | 7 cut(s) | 1065, 1901, 1953, 2232, 2292, 2358, 3024 |
| BfmI | CTRYAG | 4 cut(s) | 2163, 2524, 3134, 3417 |
| BfoI | RGCGCY | 2 cut(s) | 69, 285 |
| BfuAI | ACCTGC | 2 cut(s) | 1114, 2073 |
| BlpI | GCTNAGC | 1 cut(s) | 2859 |
| Bme1390I | CCNGG | 2 cut(s) | 987, 2091 |
| Bme18I | GGWCC | 1 cut(s) | 3392 |
| BmeRI | GACNNNNNGTC | 2 cut(s) | 379, 1532 |
| BmgT120I | GGNCC | 4 cut(s) | 498, 499, 747, 3392 |
| BmrFI | CCNGG | 2 cut(s) | 987, 2091 |
| BmrI | ACTGGG | 1 cut(s) | 2693 |
| BmtI | GCTAGC | 1 cut(s) | 3027 |
| BmuI | ACTGGG | 1 cut(s) | 2693 |
| BpiI | GAAGAC | 3 cut(s) | 1001, 3312, 3381 |
| BpmI | CTGGAG | 2 cut(s) | 68, 2231 |
| Bpu10I | CCTNAGC | 2 cut(s) | 2169, 3218 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 2859 |
| BpuEI | CTTGAG | 3 cut(s) | 1186, 2039, 2264 |
| BsaAI | YACGTR | 2 cut(s) | 434, 1718 |
| BsaHI | GRCGYC | 4 cut(s) | 48, 243, 276, 534 |
| BsaI | GGTCTC | 2 cut(s) | 490, 2475 |
| BsaJI | CCNNGG | 2 cut(s) | 822, 3049 |
| BsaWI | WCCGGW | 2 cut(s) | 830, 1251 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 23, 53, 311, 341, 2679, 2709 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 222, 481, 495, 1274, 1353, 1936, 2597, 2684, 3213 |
| Bse118I | RCCGGY | 3 cut(s) | 98, 239, 730 |
| Bse1I | ACTGG | 7 cut(s) | 58, 1474, 2314, 2516, 2688, 2762, 2818 |
| Bse21I | CCTNAGG | 1 cut(s) | 1815 |
| Bse3DI | GCAATG | 2 cut(s) | 1954, 3242 |
| BseAI | TCCGGA | 1 cut(s) | 1251 |
| BseBI | CCWGG | 2 cut(s) | 987, 2091 |
| BseDI | CCNNGG | 2 cut(s) | 822, 3049 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 222, 481, 495, 1274, 1353, 1936, 2597, 2684, 3213 |
| BseMI | GCAATG | 2 cut(s) | 1954, 3242 |
| BseMII | CTCAG | 9 cut(s) | 32, 858, 990, 1440, 1806, 2183, 2394, 2873, 3201 |
| BseNI | ACTGG | 7 cut(s) | 58, 1474, 2314, 2516, 2688, 2762, 2818 |
| BseRI | GAGGAG | 5 cut(s) | 62, 464, 1373, 3383, 3437 |
| BseSI | GKGCMC | 4 cut(s) | 502, 1483, 1702, 1930 |
| BseX3I | CGGCCG | 1 cut(s) | 100 |
| BseYI | CCCAGC | 1 cut(s) | 1929 |
| BsgI | GTGCAG | 2 cut(s) | 220, 2625 |
| Bsh1236I | CGCG | 5 cut(s) | 105, 308, 647, 1193, 1749 |
| Bsh1285I | CGRYCG | 1 cut(s) | 103 |
| BshNI | GGYRCC | 1 cut(s) | 907 |
| BsiEI | CGRYCG | 1 cut(s) | 103 |
| BsiHKAI | GWGCWC | 6 cut(s) | 1483, 1702, 1747, 1773, 3442, 3459 |
| BslFI | GGGAC | 4 cut(s) | 239, 859, 1055, 3405 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 222, 481, 495, 1274, 1353, 1936, 2597, 2684, 3213 |
| BsmAI | GTCTC | 9 cut(s) | 298, 404, 490, 1404, 1667, 2475, 2548, 2650, 3053 |
| BsmBI | CGTCTC | 2 cut(s) | 298, 404 |
| BsmFI | GGGAC | 4 cut(s) | 239, 859, 1055, 3405 |
| Bso31I | GGTCTC | 2 cut(s) | 490, 2475 |
| Bsp120I | GGGCCC | 1 cut(s) | 498 |
| Bsp1286I | GDGCHC | 9 cut(s) | 502, 1483, 1702, 1747, 1773, 1930, 3274, 3442, 3459 |
| Bsp13I | TCCGGA | 1 cut(s) | 1251 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 2859 |
| Bsp68I | TCGCGA | 1 cut(s) | 647 |
| BspCNI | CTCAG | 9 cut(s) | 31, 859, 991, 1439, 1807, 2182, 2395, 2872, 3202 |
| BspEI | TCCGGA | 1 cut(s) | 1251 |
| BspFNI | CGCG | 5 cut(s) | 105, 308, 647, 1193, 1749 |
| BspHI | TCATGA | 1 cut(s) | 2652 |
| BspMAI | CTGCAG | 3 cut(s) | 2167, 2528, 3421 |
| BspMI | ACCTGC | 2 cut(s) | 1114, 2073 |
| BspOI | GCTAGC | 1 cut(s) | 3027 |
| BspQI | GCTCTTC | 1 cut(s) | 3447 |
| BspT107I | GGYRCC | 1 cut(s) | 907 |
| BspTNI | GGTCTC | 2 cut(s) | 490, 2475 |
| BsrBI | CCGCTC | 2 cut(s) | 39, 198 |
| BsrDI | GCAATG | 2 cut(s) | 1954, 3242 |
| BsrFI | RCCGGY | 3 cut(s) | 98, 239, 730 |
| BsrI | ACTGG | 7 cut(s) | 58, 1474, 2314, 2516, 2688, 2762, 2818 |
| BssAI | RCCGGY | 3 cut(s) | 98, 239, 730 |
| BssECI | CCNNGG | 2 cut(s) | 822, 3049 |
| BssNAI | GTATAC | 1 cut(s) | 3471 |
| BssNI | GRCGYC | 4 cut(s) | 48, 243, 276, 534 |
| BssSI | CACGAG | 1 cut(s) | 443 |
| BssT1I | CCWWGG | 2 cut(s) | 822, 3049 |
| Bst1107I | GTATAC | 1 cut(s) | 3471 |
| Bst2BI | CACGAG | 1 cut(s) | 443 |
| Bst2UI | CCWGG | 2 cut(s) | 987, 2091 |
| Bst6I | CTCTTC | 4 cut(s) | 17, 132, 1980, 3447 |
| BstACI | GRCGYC | 4 cut(s) | 48, 243, 276, 534 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 907, 1690 |
| BstBAI | YACGTR | 2 cut(s) | 434, 1718 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 479 |
| BstFNI | CGCG | 5 cut(s) | 105, 308, 647, 1193, 1749 |
| BstH2I | RGCGCY | 2 cut(s) | 69, 285 |
| BstHHI | GCGC | 4 cut(s) | 68, 284, 1851, 2564 |
| BstMAI | GTCTC | 9 cut(s) | 298, 404, 490, 1404, 1667, 2475, 2548, 2650, 3053 |
| BstMCI | CGRYCG | 1 cut(s) | 103 |
| BstNI | CCWGG | 2 cut(s) | 987, 2091 |
| BstNSI | RCATGY | 5 cut(s) | 520, 967, 1189, 1203, 3181 |
| BstSCI | CCNGG | 2 cut(s) | 985, 2089 |
| BstSFI | CTRYAG | 4 cut(s) | 2163, 2524, 3134, 3417 |
| BstSLI | GKGCMC | 4 cut(s) | 502, 1483, 1702, 1930 |
| BstUI | CGCG | 5 cut(s) | 105, 308, 647, 1193, 1749 |
| BstV2I | GAAGAC | 3 cut(s) | 1001, 3312, 3381 |
| BstX2I | RGATCY | 8 cut(s) | 21, 931, 1792, 1819, 2512, 2641, 3125, 3222 |
| BstYI | RGATCY | 8 cut(s) | 21, 931, 1792, 1819, 2512, 2641, 3125, 3222 |
| BstZ17I | GTATAC | 1 cut(s) | 3471 |
| BstZI | CGGCCG | 1 cut(s) | 100 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1815 |
| BtgZI | GCGATG | 1 cut(s) | 3069 |
| BtsI | GCAGTG | 3 cut(s) | 2323, 2533, 3459 |
| BtsIMutI | CAGTG | 9 cut(s) | 637, 1481, 1823, 2323, 2533, 2811, 3156, 3316, 3459 |
| BtuMI | TCGCGA | 1 cut(s) | 647 |
| BveI | ACCTGC | 2 cut(s) | 1114, 2073 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1499, 1969 |
| CciI | TCATGA | 1 cut(s) | 2652 |
| CfoI | GCGC | 4 cut(s) | 68, 284, 1851, 2564 |
| Cfr10I | RCCGGY | 3 cut(s) | 98, 239, 730 |
| Cfr13I | GGNCC | 4 cut(s) | 498, 499, 747, 3392 |
| CseI | GACGC | 5 cut(s) | 56, 232, 421, 542, 907 |
| Csp6I | GTAC | 5 cut(s) | 970, 2519, 2809, 2817, 3175 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 2909, 2944 |
| CviAII | CATG | 9 cut(s) | 517, 964, 1186, 1200, 1530, 2653, 3007, 3070, 3178 |
| CviQI | GTAC | 5 cut(s) | 970, 2519, 2809, 2817, 3175 |
| DraI | TTTAAA | 2 cut(s) | 1138, 1668 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 113, 275 |
| DriI | GACNNNNNGTC | 2 cut(s) | 379, 1532 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 113, 275 |
| EaeI | YGGCCR | 6 cut(s) | 100, 508, 1272, 1552, 2087, 2783 |
| EagI | CGGCCG | 1 cut(s) | 100 |
| Eam1104I | CTCTTC | 4 cut(s) | 17, 132, 1980, 3447 |
| Eam1105I | GACNNNNNGTC | 2 cut(s) | 379, 1532 |
| EarI | CTCTTC | 4 cut(s) | 17, 132, 1980, 3447 |
| EciI | GGCGGA | 4 cut(s) | 44, 82, 118, 151 |
| EclXI | CGGCCG | 1 cut(s) | 100 |
| Eco130I | CCWWGG | 2 cut(s) | 822, 3049 |
| Eco147I | AGGCCT | 2 cut(s) | 2224, 2677 |
| Eco24I | GRGCYC | 2 cut(s) | 502, 3274 |
| Eco31I | GGTCTC | 2 cut(s) | 490, 2475 |
| Eco32I | GATATC | 1 cut(s) | 2373 |
| Eco47I | GGWCC | 1 cut(s) | 3392 |
| Eco52I | CGGCCG | 1 cut(s) | 100 |
| Eco57I | CTGAAG | 4 cut(s) | 1863, 2595, 2655, 3204 |
| Eco72I | CACGTG | 1 cut(s) | 434 |
| Eco81I | CCTNAGG | 1 cut(s) | 1815 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 479 |
| EcoO109I | RGGNCCY | 1 cut(s) | 3392 |
| EcoRI | GAATTC | 1 cut(s) | 637 |
| EcoRII | CCWGG | 2 cut(s) | 985, 2089 |
| EcoRV | GATATC | 1 cut(s) | 2373 |
| EcoT14I | CCWWGG | 2 cut(s) | 822, 3049 |
| EcoT22I | ATGCAT | 1 cut(s) | 3106 |
| EcoT38I | GRGCYC | 2 cut(s) | 502, 3274 |
| ErhI | CCWWGG | 2 cut(s) | 822, 3049 |
| Esp3I | CGTCTC | 2 cut(s) | 298, 404 |
| FaeI | CATG | 9 cut(s) | 520, 967, 1189, 1203, 1533, 2656, 3010, 3073, 3181 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 167, 199, 2593, 2625 |
| FaqI | GGGAC | 4 cut(s) | 239, 859, 1055, 3405 |
| FatI | CATG | 9 cut(s) | 516, 963, 1185, 1199, 1529, 2652, 3006, 3069, 3177 |
| FauI | CCCGC | 3 cut(s) | 163, 212, 509 |
| FauNDI | CATATG | 2 cut(s) | 1119, 1462 |
| FblI | GTMKAC | 5 cut(s) | 1525, 1714, 2270, 3045, 3470 |
| FriOI | GRGCYC | 2 cut(s) | 502, 3274 |
| FspBI | CTAG | 7 cut(s) | 1065, 1901, 1953, 2232, 2292, 2358, 3024 |
| FspI | TGCGCA | 1 cut(s) | 1850 |
| GlaI | GCGC | 4 cut(s) | 67, 283, 1850, 2563 |
| GsaI | CCCAGC | 1 cut(s) | 1933 |
| GsuI | CTGGAG | 2 cut(s) | 68, 2231 |
| HaeII | RGCGCY | 2 cut(s) | 69, 285 |
| HgaI | GACGC | 5 cut(s) | 56, 232, 421, 542, 907 |
| HhaI | GCGC | 4 cut(s) | 68, 284, 1851, 2564 |
| Hin1I | GRCGYC | 4 cut(s) | 48, 243, 276, 534 |
| Hin1II | CATG | 9 cut(s) | 520, 967, 1189, 1203, 1533, 2656, 3010, 3073, 3181 |
| Hin6I | GCGC | 4 cut(s) | 66, 282, 1849, 2562 |
| HinP1I | GCGC | 4 cut(s) | 66, 282, 1849, 2562 |
| HincII | GTYRAC | 3 cut(s) | 2253, 3046, 3414 |
| HindII | GTYRAC | 3 cut(s) | 2253, 3046, 3414 |
| Hpy99I | CGWCG | 5 cut(s) | 50, 137, 149, 536, 803 |
| HpyCH4IV | ACGT | 4 cut(s) | 276, 433, 1717, 1779 |
| HpySE526I | ACGT | 4 cut(s) | 276, 433, 1717, 1779 |
| Hsp92I | GRCGYC | 4 cut(s) | 48, 243, 276, 534 |
| Hsp92II | CATG | 9 cut(s) | 520, 967, 1189, 1203, 1533, 2656, 3010, 3073, 3181 |
| HspAI | GCGC | 4 cut(s) | 66, 282, 1849, 2562 |
| Kpn2I | TCCGGA | 1 cut(s) | 1251 |
| KroI | GCCGGC | 1 cut(s) | 98 |
| KroNI | GCCGGC | 1 cut(s) | 100 |
| LguI | GCTCTTC | 1 cut(s) | 3447 |
| MaeI | CTAG | 7 cut(s) | 1065, 1901, 1953, 2232, 2292, 2358, 3024 |
| MaeII | ACGT | 4 cut(s) | 276, 433, 1717, 1779 |
| MbiI | CCGCTC | 2 cut(s) | 39, 198 |
| MfeI | CAATTG | 2 cut(s) | 658, 1566 |
| MflI | RGATCY | 8 cut(s) | 21, 931, 1792, 1819, 2512, 2641, 3125, 3222 |
| MhlI | GDGCHC | 9 cut(s) | 502, 1483, 1702, 1747, 1773, 1930, 3274, 3442, 3459 |
| MlsI | TGGCCA | 2 cut(s) | 2089, 2785 |
| MluI | ACGCGT | 1 cut(s) | 1191 |
| MluNI | TGGCCA | 2 cut(s) | 2089, 2785 |
| MlyI | GAGTC | 5 cut(s) | 368, 442, 566, 2103, 2276 |
| MmeI | TCCRAC | 5 cut(s) | 66, 366, 555, 1770, 3126 |
| Mox20I | TGGCCA | 2 cut(s) | 2089, 2785 |
| Mph1103I | ATGCAT | 1 cut(s) | 3106 |
| MroI | TCCGGA | 1 cut(s) | 1251 |
| MroNI | GCCGGC | 1 cut(s) | 98 |
| MscI | TGGCCA | 2 cut(s) | 2089, 2785 |
| MseI | TTAA | 5 cut(s) | 1137, 1667, 2393, 2399, 2672 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1608 |
| Msp20I | TGGCCA | 2 cut(s) | 2089, 2785 |
| MspA1I | CMGCKG | 3 cut(s) | 1933, 2162, 3209 |
| MspR9I | CCNGG | 2 cut(s) | 987, 2091 |
| MunI | CAATTG | 2 cut(s) | 658, 1566 |
| MvaI | CCWGG | 2 cut(s) | 987, 2091 |
| MvnI | CGCG | 5 cut(s) | 105, 308, 647, 1193, 1749 |
| NaeI | GCCGGC | 1 cut(s) | 100 |
| NdeI | CATATG | 2 cut(s) | 1119, 1462 |
| NgoMIV | GCCGGC | 1 cut(s) | 98 |
| NheI | GCTAGC | 1 cut(s) | 3023 |
| NlaIII | CATG | 9 cut(s) | 520, 967, 1189, 1203, 1533, 2656, 3010, 3073, 3181 |
| NmeAIII | GCCGAG | 1 cut(s) | 396 |
| NmuCI | GTSAC | 7 cut(s) | 434, 445, 466, 895, 1333, 2095, 2624 |
| NruI | TCGCGA | 1 cut(s) | 647 |
| NsbI | TGCGCA | 1 cut(s) | 1850 |
| NsiI | ATGCAT | 1 cut(s) | 3106 |
| NspI | RCATGY | 5 cut(s) | 520, 967, 1189, 1203, 3181 |
| PaeI | GCATGC | 1 cut(s) | 1189 |
| PagI | TCATGA | 1 cut(s) | 2652 |
| PaqCI | CACCTGC | 1 cut(s) | 2073 |
| PceI | AGGCCT | 2 cut(s) | 2224, 2677 |
| PciI | ACATGT | 1 cut(s) | 963 |
| PciSI | GCTCTTC | 1 cut(s) | 3447 |
| PcsI | WCGNNNNNNNCGW | 7 cut(s) | 141, 264, 435, 528, 1022, 2491, 3051 |
| PdiI | GCCGGC | 1 cut(s) | 100 |
| PfeI | GAWTC | 7 cut(s) | 649, 1027, 1175, 1883, 2012, 2156, 2354 |
| PflFI | GACNNNGTC | 2 cut(s) | 230, 683 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1936 |
| PleI | GAGTC | 5 cut(s) | 368, 442, 565, 2102, 2275 |
| PmaCI | CACGTG | 1 cut(s) | 434 |
| PmlI | CACGTG | 1 cut(s) | 434 |
| PpsI | GAGTC | 5 cut(s) | 368, 442, 565, 2102, 2275 |
| Ppu21I | YACGTR | 2 cut(s) | 434, 1718 |
| PpuMI | RGGWCCY | 1 cut(s) | 3392 |
| PscI | ACATGT | 1 cut(s) | 963 |
| Psp5II | RGGWCCY | 1 cut(s) | 3392 |
| Psp6I | CCWGG | 2 cut(s) | 985, 2089 |
| PspCI | CACGTG | 1 cut(s) | 434 |
| PspFI | CCCAGC | 1 cut(s) | 1929 |
| PspGI | CCWGG | 2 cut(s) | 985, 2089 |
| PspOMI | GGGCCC | 1 cut(s) | 498 |
| PspPI | GGNCC | 4 cut(s) | 498, 499, 747, 3392 |
| PspPPI | RGGWCCY | 1 cut(s) | 3392 |
| PstI | CTGCAG | 3 cut(s) | 2167, 2528, 3421 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1499, 1969 |
| PsuI | RGATCY | 8 cut(s) | 21, 931, 1792, 1819, 2512, 2641, 3125, 3222 |
| PsyI | GACNNNGTC | 2 cut(s) | 230, 683 |
| PvuII | CAGCTG | 1 cut(s) | 2162 |
| RruI | TCGCGA | 1 cut(s) | 647 |
| RsaI | GTAC | 5 cut(s) | 971, 2520, 2810, 2818, 3176 |
| RsaNI | GTAC | 5 cut(s) | 970, 2519, 2809, 2817, 3175 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1608 |
| SalI | GTCGAC | 1 cut(s) | 3044 |
| SapI | GCTCTTC | 1 cut(s) | 3447 |
| SaqAI | TTAA | 5 cut(s) | 1137, 1667, 2393, 2399, 2672 |
| Sau96I | GGNCC | 4 cut(s) | 498, 499, 747, 3392 |
| SchI | GAGTC | 5 cut(s) | 368, 442, 566, 2103, 2276 |
| ScrFI | CCNGG | 2 cut(s) | 987, 2091 |
| SduI | GDGCHC | 9 cut(s) | 502, 1483, 1702, 1747, 1773, 1930, 3274, 3442, 3459 |
| SfcI | CTRYAG | 4 cut(s) | 2163, 2524, 3134, 3417 |
| SgrAI | CRCCGGYG | 1 cut(s) | 239 |
| SinI | GGWCC | 1 cut(s) | 3392 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1608 |
| SmlI | CTYRAG | 3 cut(s) | 1165, 2054, 2279 |
| SmoI | CTYRAG | 3 cut(s) | 1165, 2054, 2279 |
| SphI | GCATGC | 1 cut(s) | 1189 |
| SseBI | AGGCCT | 2 cut(s) | 2224, 2677 |
| SspMI | CTAG | 7 cut(s) | 1065, 1901, 1953, 2232, 2292, 2358, 3024 |
| StuI | AGGCCT | 2 cut(s) | 2224, 2677 |
| StyD4I | CCNGG | 2 cut(s) | 985, 2089 |
| StyI | CCWWGG | 2 cut(s) | 822, 3049 |
| TaiI | ACGT | 4 cut(s) | 279, 436, 1720, 1782 |
| TatI | WGTACW | 3 cut(s) | 969, 2808, 3174 |
| TauI | GCSGC | 6 cut(s) | 98, 105, 287, 290, 308, 3209 |
| TfiI | GAWTC | 7 cut(s) | 649, 1027, 1175, 1883, 2012, 2156, 2354 |
| Tru1I | TTAA | 5 cut(s) | 1137, 1667, 2393, 2399, 2672 |
| Tru9I | TTAA | 5 cut(s) | 1137, 1667, 2393, 2399, 2672 |
| TscAI | CASTG | 9 cut(s) | 637, 1488, 1830, 2323, 2533, 2818, 3163, 3316, 3459 |
| TseFI | GTSAC | 7 cut(s) | 434, 445, 466, 895, 1333, 2095, 2624 |
| Tsp45I | GTSAC | 7 cut(s) | 434, 445, 466, 895, 1333, 2095, 2624 |
| TspDTI | ATGAA | 8 cut(s) | 1091, 1106, 1119, 1863, 2186, 2634, 2844, 3204 |
| TspGWI | ACGGA | 4 cut(s) | 103, 787, 2747, 3250 |
| TspRI | CASTG | 9 cut(s) | 637, 1488, 1830, 2323, 2533, 2818, 3163, 3316, 3459 |
| Tth111I | GACNNNGTC | 2 cut(s) | 230, 683 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1936 |
| VneI | GTGCAC | 2 cut(s) | 1479, 1698 |
| VpaK11BI | GGWCC | 1 cut(s) | 3392 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 479 |
| XapI | RAATTY | 7 cut(s) | 637, 739, 1662, 1870, 2721, 2768, 3080 |
| XbaI | TCTAGA | 1 cut(s) | 1900 |
| XceI | RCATGY | 5 cut(s) | 520, 967, 1189, 1203, 3181 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 1536, 2082 |
| XmiI | GTMKAC | 5 cut(s) | 1525, 1714, 2270, 3045, 3470 |
| XspI | CTAG | 7 cut(s) | 1065, 1901, 1953, 2232, 2292, 2358, 3024 |
| ZraI | GACGTC | 1 cut(s) | 277 |
| Zsp2I | ATGCAT | 1 cut(s) | 3106 |