TPLATE-like
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT3G01780 AT3G01780 |
| fragaria_vesca | FvH4_5g15940 |
| malus_domestica | MD06G1184100.v1.1 MD14G1190000.v1.1 |
| prunus_persica | Prupe.5G183000_v2.0.a1 |
| pyrus_communis | pycom06g16320 pycom06g16330 pycom14g15780 |
| rosa_chinensis | RchiOBHm_Chr7g0180111 |
| rosa_laevigata | RLG00000005292 |
| rosa_multiflora | Rmu_sc0017616.1_g000007 Rmu_ssc0000119.1_g000034 |
| rosa_roxburghii | Rroxscaffold_3G00273120 |
| rosa_rugosa | Rorug06G0430700 |
| rosa_samantha | Rh7AG031100 Rh7BG031000 Rh7CG033000 Rh7DG031600 |
| rosa_wichuraiana | Rw7G002580 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 2073 |
| AasI | GACNNNNNNGTC | 2 cut(s) | 113, 275 |
| AatII | GACGTC | 1 cut(s) | 279 |
| Acc36I | ACCTGC | 3 cut(s) | 1114, 2073, 2488 |
| AccB1I | GGYRCC | 2 cut(s) | 65, 907 |
| AccBSI | CCGCTC | 2 cut(s) | 39, 198 |
| AccI | GTMKAC | 4 cut(s) | 1714, 2270, 3159, 3581 |
| AccII | CGCG | 5 cut(s) | 105, 205, 401, 551, 1749 |
| AcoI | YGGCCR | 4 cut(s) | 100, 508, 1552, 2087 |
| AcsI | RAATTY | 5 cut(s) | 637, 1662, 2491, 2882, 3194 |
| AcuI | CTGAAG | 3 cut(s) | 1863, 2769, 3318 |
| AcvI | CACGTG | 1 cut(s) | 434 |
| AcyI | GRCGYC | 5 cut(s) | 48, 66, 243, 276, 534 |
| AfaI | GTAC | 5 cut(s) | 971, 2631, 2924, 2932, 3290 |
| AfeI | AGCGCT | 1 cut(s) | 1218 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 222, 495, 1274, 1353, 2708, 2798 |
| AflIII | ACRYGT | 2 cut(s) | 963, 3049 |
| AgeI | ACCGGT | 2 cut(s) | 2309, 2875 |
| AhdI | GACNNNNNGTC | 2 cut(s) | 379, 1532 |
| AjnI | CCWGG | 1 cut(s) | 985 |
| Alw21I | GWGCWC | 6 cut(s) | 1483, 1747, 1773, 1930, 3553, 3570 |
| Alw44I | GTGCAC | 1 cut(s) | 1479 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1499, 1969 |
| Aor51HI | AGCGCT | 1 cut(s) | 1218 |
| ApaLI | GTGCAC | 1 cut(s) | 1479 |
| ApoI | RAATTY | 5 cut(s) | 637, 1662, 2491, 2882, 3194 |
| ArsI | GACNNNNNNTTYG | 3 cut(s) | 28, 129, 161 |
| AsiGI | ACCGGT | 2 cut(s) | 2309, 2875 |
| AspLEI | GCGC | 5 cut(s) | 68, 207, 284, 1219, 2675 |
| AspS9I | GGNCC | 5 cut(s) | 499, 747, 1657, 2898, 3503 |
| AsuC2I | CCSGG | 1 cut(s) | 2091 |
| AsuII | TTCGAA | 1 cut(s) | 542 |
| AvaII | GGWCC | 2 cut(s) | 1657, 3503 |
| AxyI | CCTNAGG | 1 cut(s) | 1815 |
| BaeGI | GKGCMC | 2 cut(s) | 912, 1483 |
| BamHI | GGATCC | 4 cut(s) | 931, 1792, 1819, 2623 |
| BanI | GGYRCC | 2 cut(s) | 65, 907 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 3095, 3127 |
| BauI | CACGAG | 1 cut(s) | 443 |
| BbrPI | CACGTG | 1 cut(s) | 434 |
| BbsI | GAAGAC | 3 cut(s) | 819, 2451, 3423 |
| Bbv12I | GWGCWC | 6 cut(s) | 1483, 1747, 1773, 1930, 3553, 3570 |
| BceAI | ACGGC | 8 cut(s) | 116, 149, 275, 364, 521, 541, 758, 2019 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 160, 194, 2144, 2178 |
| BciT130I | CCWGG | 1 cut(s) | 987 |
| BcnI | CCSGG | 1 cut(s) | 2091 |
| BfaI | CTAG | 5 cut(s) | 1065, 1901, 1953, 2232, 3138 |
| BfmI | CTRYAG | 4 cut(s) | 2163, 2635, 3248, 3528 |
| BfoI | RGCGCY | 3 cut(s) | 69, 285, 1220 |
| BfuAI | ACCTGC | 3 cut(s) | 1114, 2073, 2488 |
| BglI | GCCNNNNNGGC | 3 cut(s) | 63, 507, 779 |
| BglII | AGATCT | 1 cut(s) | 3244 |
| BlpI | GCTNAGC | 2 cut(s) | 1929, 2973 |
| BmcAI | AGTACT | 1 cut(s) | 2631 |
| Bme1390I | CCNGG | 2 cut(s) | 987, 2091 |
| Bme18I | GGWCC | 2 cut(s) | 1657, 3503 |
| BmeRI | GACNNNNNGTC | 2 cut(s) | 379, 1532 |
| BmgT120I | GGNCC | 5 cut(s) | 499, 747, 1657, 2898, 3503 |
| BmrFI | CCNGG | 2 cut(s) | 987, 2091 |
| BmrI | ACTGGG | 2 cut(s) | 2807, 2895 |
| BmsI | GCATC | 7 cut(s) | 1294, 1379, 2035, 2660, 2996, 3205, 3227 |
| BmuI | ACTGGG | 2 cut(s) | 2807, 2895 |
| BpiI | GAAGAC | 3 cut(s) | 819, 2451, 3423 |
| BpmI | CTGGAG | 2 cut(s) | 25, 68 |
| Bpu10I | CCTNAGC | 2 cut(s) | 2169, 3332 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 1929, 2973 |
| Bpu14I | TTCGAA | 1 cut(s) | 542 |
| BpuEI | CTTGAG | 3 cut(s) | 1186, 2039, 2264 |
| BpuMI | CCSGG | 1 cut(s) | 2091 |
| Bsa29I | ATCGAT | 1 cut(s) | 1016 |
| BsaAI | YACGTR | 2 cut(s) | 434, 1718 |
| BsaHI | GRCGYC | 5 cut(s) | 48, 66, 243, 276, 534 |
| BsaI | GGTCTC | 3 cut(s) | 490, 670, 2586 |
| BsaJI | CCNNGG | 3 cut(s) | 750, 1455, 3163 |
| BsaWI | WCCGGW | 3 cut(s) | 393, 2309, 2875 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 23, 53, 1987, 2017 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 222, 495, 1274, 1353, 2708, 2798 |
| Bse118I | RCCGGY | 5 cut(s) | 98, 239, 730, 2309, 2875 |
| Bse1I | ACTGG | 7 cut(s) | 58, 1474, 2358, 2627, 2802, 2901, 2932 |
| Bse21I | CCTNAGG | 1 cut(s) | 1815 |
| Bse3DI | GCAATG | 1 cut(s) | 3356 |
| BseBI | CCWGG | 1 cut(s) | 987 |
| BseCI | ATCGAT | 1 cut(s) | 1016 |
| BseDI | CCNNGG | 3 cut(s) | 750, 1455, 3163 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 222, 495, 1274, 1353, 2708, 2798 |
| BseMI | GCAATG | 1 cut(s) | 3356 |
| BseNI | ACTGG | 7 cut(s) | 58, 1474, 2358, 2627, 2802, 2901, 2932 |
| BseRI | GAGGAG | 3 cut(s) | 62, 1373, 3548 |
| BseSI | GKGCMC | 2 cut(s) | 912, 1483 |
| BseX3I | CGGCCG | 1 cut(s) | 100 |
| BsgI | GTGCAG | 2 cut(s) | 220, 2739 |
| Bsh1236I | CGCG | 5 cut(s) | 105, 205, 401, 551, 1749 |
| Bsh1285I | CGRYCG | 1 cut(s) | 103 |
| BshNI | GGYRCC | 2 cut(s) | 65, 907 |
| BshTI | ACCGGT | 2 cut(s) | 2309, 2875 |
| BshVI | ATCGAT | 1 cut(s) | 1016 |
| BsiEI | CGRYCG | 1 cut(s) | 103 |
| BsiHKAI | GWGCWC | 6 cut(s) | 1483, 1747, 1773, 1930, 3553, 3570 |
| BslFI | GGGAC | 2 cut(s) | 239, 3516 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 222, 495, 1274, 1353, 2708, 2798 |
| BsmBI | CGTCTC | 2 cut(s) | 298, 404 |
| BsmFI | GGGAC | 2 cut(s) | 239, 3516 |
| BsmI | GAATGC | 1 cut(s) | 423 |
| Bso31I | GGTCTC | 3 cut(s) | 490, 670, 2586 |
| Bsp119I | TTCGAA | 1 cut(s) | 542 |
| Bsp1286I | GDGCHC | 7 cut(s) | 912, 1483, 1747, 1773, 1930, 3553, 3570 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 1929, 2973 |
| Bsp68I | TCGCGA | 1 cut(s) | 401 |
| BspDI | ATCGAT | 1 cut(s) | 1016 |
| BspFNI | CGCG | 5 cut(s) | 105, 205, 401, 551, 1749 |
| BspHI | TCATGA | 1 cut(s) | 2766 |
| BspMAI | CTGCAG | 3 cut(s) | 2167, 2639, 3532 |
| BspMI | ACCTGC | 3 cut(s) | 1114, 2073, 2488 |
| BspQI | GCTCTTC | 1 cut(s) | 3558 |
| BspT104I | TTCGAA | 1 cut(s) | 542 |
| BspT107I | GGYRCC | 2 cut(s) | 65, 907 |
| BspTNI | GGTCTC | 3 cut(s) | 490, 670, 2586 |
| BsrBI | CCGCTC | 2 cut(s) | 39, 198 |
| BsrDI | GCAATG | 1 cut(s) | 3356 |
| BsrFI | RCCGGY | 5 cut(s) | 98, 239, 730, 2309, 2875 |
| BsrI | ACTGG | 7 cut(s) | 58, 1474, 2358, 2627, 2802, 2901, 2932 |
| BssAI | RCCGGY | 5 cut(s) | 98, 239, 730, 2309, 2875 |
| BssECI | CCNNGG | 3 cut(s) | 750, 1455, 3163 |
| BssNAI | GTATAC | 1 cut(s) | 3582 |
| BssNI | GRCGYC | 5 cut(s) | 48, 66, 243, 276, 534 |
| BssSI | CACGAG | 1 cut(s) | 443 |
| BssT1I | CCWWGG | 2 cut(s) | 1455, 3163 |
| Bst1107I | GTATAC | 1 cut(s) | 3582 |
| Bst2BI | CACGAG | 1 cut(s) | 443 |
| Bst2UI | CCWGG | 1 cut(s) | 987 |
| Bst4CI | ACNGT | 9 cut(s) | 232, 754, 2143, 2450, 2565, 2647, 3064, 3422, 3548 |
| Bst6I | CTCTTC | 8 cut(s) | 17, 132, 673, 1249, 1980, 2250, 2771, 3558 |
| BstACI | GRCGYC | 5 cut(s) | 48, 66, 243, 276, 534 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 907, 1690 |
| BstBAI | YACGTR | 2 cut(s) | 434, 1718 |
| BstBI | TTCGAA | 1 cut(s) | 542 |
| BstC8I | GCNNGC | 9 cut(s) | 100, 180, 645, 1187, 1191, 1500, 1747, 1803, 2397 |
| BstDSI | CCRYGG | 1 cut(s) | 750 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1351 |
| BstFNI | CGCG | 5 cut(s) | 105, 205, 401, 551, 1749 |
| BstH2I | RGCGCY | 3 cut(s) | 69, 285, 1220 |
| BstHHI | GCGC | 5 cut(s) | 68, 207, 284, 1219, 2675 |
| BstMCI | CGRYCG | 1 cut(s) | 103 |
| BstNI | CCWGG | 1 cut(s) | 987 |
| BstNSI | RCATGY | 7 cut(s) | 520, 967, 1189, 1193, 1203, 3053, 3295 |
| BstSCI | CCNGG | 2 cut(s) | 985, 2089 |
| BstSFI | CTRYAG | 4 cut(s) | 2163, 2635, 3248, 3528 |
| BstSLI | GKGCMC | 2 cut(s) | 912, 1483 |
| BstUI | CGCG | 5 cut(s) | 105, 205, 401, 551, 1749 |
| BstV2I | GAAGAC | 3 cut(s) | 819, 2451, 3423 |
| BstX2I | RGATCY | 8 cut(s) | 21, 931, 1792, 1819, 2623, 2755, 3244, 3336 |
| BstYI | RGATCY | 8 cut(s) | 21, 931, 1792, 1819, 2623, 2755, 3244, 3336 |
| BstZ17I | GTATAC | 1 cut(s) | 3582 |
| BstZI | CGGCCG | 1 cut(s) | 100 |
| Bsu15I | ATCGAT | 1 cut(s) | 1016 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1815 |
| BsuTUI | ATCGAT | 1 cut(s) | 1016 |
| BtgI | CCRYGG | 1 cut(s) | 750 |
| BtgZI | GCGATG | 2 cut(s) | 103, 322 |
| BtsI | GCAGTG | 3 cut(s) | 2323, 2644, 3570 |
| BtuMI | TCGCGA | 1 cut(s) | 401 |
| BveI | ACCTGC | 3 cut(s) | 1114, 2073, 2488 |
| Cac8I | GCNNGC | 9 cut(s) | 100, 180, 645, 1187, 1191, 1500, 1747, 1803, 2397 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1499, 1969 |
| CciI | TCATGA | 1 cut(s) | 2766 |
| CfoI | GCGC | 5 cut(s) | 68, 207, 284, 1219, 2675 |
| Cfr10I | RCCGGY | 5 cut(s) | 98, 239, 730, 2309, 2875 |
| Cfr13I | GGNCC | 5 cut(s) | 499, 747, 1657, 2898, 3503 |
| ClaI | ATCGAT | 1 cut(s) | 1016 |
| CseI | GACGC | 4 cut(s) | 56, 232, 542, 907 |
| Csp6I | GTAC | 5 cut(s) | 970, 2630, 2923, 2931, 3289 |
| CspAI | ACCGGT | 2 cut(s) | 2309, 2875 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 3023, 3058 |
| CviQI | GTAC | 5 cut(s) | 970, 2630, 2923, 2931, 3289 |
| DinI | GGCGCC | 1 cut(s) | 67 |
| DraI | TTTAAA | 1 cut(s) | 1668 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 113, 275 |
| DriI | GACNNNNNGTC | 2 cut(s) | 379, 1532 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 113, 275 |
| EaeI | YGGCCR | 4 cut(s) | 100, 508, 1552, 2087 |
| EagI | CGGCCG | 1 cut(s) | 100 |
| Eam1104I | CTCTTC | 8 cut(s) | 17, 132, 673, 1249, 1980, 2250, 2771, 3558 |
| Eam1105I | GACNNNNNGTC | 2 cut(s) | 379, 1532 |
| EarI | CTCTTC | 8 cut(s) | 17, 132, 673, 1249, 1980, 2250, 2771, 3558 |
| EciI | GGCGGA | 4 cut(s) | 44, 82, 118, 151 |
| EclXI | CGGCCG | 1 cut(s) | 100 |
| Eco130I | CCWWGG | 2 cut(s) | 1455, 3163 |
| Eco147I | AGGCCT | 1 cut(s) | 2791 |
| Eco31I | GGTCTC | 3 cut(s) | 490, 670, 2586 |
| Eco32I | GATATC | 1 cut(s) | 2373 |
| Eco47I | GGWCC | 2 cut(s) | 1657, 3503 |
| Eco47III | AGCGCT | 1 cut(s) | 1218 |
| Eco52I | CGGCCG | 1 cut(s) | 100 |
| Eco57I | CTGAAG | 3 cut(s) | 1863, 2769, 3318 |
| Eco72I | CACGTG | 1 cut(s) | 434 |
| Eco81I | CCTNAGG | 1 cut(s) | 1815 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1351 |
| EcoO109I | RGGNCCY | 1 cut(s) | 3503 |
| EcoRI | GAATTC | 1 cut(s) | 637 |
| EcoRII | CCWGG | 1 cut(s) | 985 |
| EcoRV | GATATC | 1 cut(s) | 2373 |
| EcoT14I | CCWWGG | 2 cut(s) | 1455, 3163 |
| EcoT22I | ATGCAT | 2 cut(s) | 1191, 3220 |
| EgeI | GGCGCC | 1 cut(s) | 67 |
| EheI | GGCGCC | 1 cut(s) | 67 |
| ErhI | CCWWGG | 2 cut(s) | 1455, 3163 |
| Esp3I | CGTCTC | 2 cut(s) | 298, 404 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 167, 199, 1308, 1340, 2359, 2391 |
| FaqI | GGGAC | 2 cut(s) | 239, 3516 |
| FauI | CCCGC | 1 cut(s) | 163 |
| FauNDI | CATATG | 2 cut(s) | 1119, 1462 |
| FblI | GTMKAC | 4 cut(s) | 1714, 2270, 3159, 3581 |
| FspBI | CTAG | 5 cut(s) | 1065, 1901, 1953, 2232, 3138 |
| GlaI | GCGC | 5 cut(s) | 67, 206, 283, 1218, 2674 |
| GsuI | CTGGAG | 2 cut(s) | 25, 68 |
| HaeII | RGCGCY | 3 cut(s) | 69, 285, 1220 |
| HgaI | GACGC | 4 cut(s) | 56, 232, 542, 907 |
| HhaI | GCGC | 5 cut(s) | 68, 207, 284, 1219, 2675 |
| Hin1I | GRCGYC | 5 cut(s) | 48, 66, 243, 276, 534 |
| Hin6I | GCGC | 5 cut(s) | 66, 205, 282, 1217, 2673 |
| HinP1I | GCGC | 5 cut(s) | 66, 205, 282, 1217, 2673 |
| HincII | GTYRAC | 3 cut(s) | 2253, 2844, 3160 |
| HindII | GTYRAC | 3 cut(s) | 2253, 2844, 3160 |
| HindIII | AAGCTT | 1 cut(s) | 2393 |
| Hpy99I | CGWCG | 6 cut(s) | 50, 137, 149, 527, 803, 881 |
| HpyCH4III | ACNGT | 9 cut(s) | 232, 754, 2143, 2450, 2565, 2647, 3064, 3422, 3548 |
| HpyCH4IV | ACGT | 4 cut(s) | 276, 433, 1696, 1717 |
| HpySE526I | ACGT | 4 cut(s) | 276, 433, 1696, 1717 |
| Hsp92I | GRCGYC | 5 cut(s) | 48, 66, 243, 276, 534 |
| HspAI | GCGC | 5 cut(s) | 66, 205, 282, 1217, 2673 |
| KasI | GGCGCC | 1 cut(s) | 65 |
| KroI | GCCGGC | 1 cut(s) | 98 |
| KroNI | GCCGGC | 1 cut(s) | 100 |
| LguI | GCTCTTC | 1 cut(s) | 3558 |
| LmnI | GCTCC | 8 cut(s) | 44, 203, 800, 1451, 1995, 2984, 3134, 3573 |
| LweI | GCATC | 7 cut(s) | 1294, 1379, 2035, 2660, 2996, 3205, 3227 |
| MaeI | CTAG | 5 cut(s) | 1065, 1901, 1953, 2232, 3138 |
| MaeII | ACGT | 4 cut(s) | 276, 433, 1696, 1717 |
| MbiI | CCGCTC | 2 cut(s) | 39, 198 |
| MfeI | CAATTG | 3 cut(s) | 658, 1566, 2600 |
| MflI | RGATCY | 8 cut(s) | 21, 931, 1792, 1819, 2623, 2755, 3244, 3336 |
| MhlI | GDGCHC | 7 cut(s) | 912, 1483, 1747, 1773, 1930, 3553, 3570 |
| Mly113I | GGCGCC | 1 cut(s) | 66 |
| MlyI | GAGTC | 7 cut(s) | 368, 442, 566, 600, 2046, 2276, 3379 |
| MmeI | TCCRAC | 3 cut(s) | 859, 1770, 3240 |
| Mph1103I | ATGCAT | 2 cut(s) | 1191, 3220 |
| MroNI | GCCGGC | 1 cut(s) | 98 |
| MseI | TTAA | 9 cut(s) | 707, 777, 1137, 1326, 1667, 2412, 2504, 2510, 2786 |
| MspA1I | CMGCKG | 2 cut(s) | 1798, 1933 |
| MspR9I | CCNGG | 2 cut(s) | 987, 2091 |
| MunI | CAATTG | 3 cut(s) | 658, 1566, 2600 |
| Mva1269I | GAATGC | 1 cut(s) | 423 |
| MvaI | CCWGG | 1 cut(s) | 987 |
| MvnI | CGCG | 5 cut(s) | 105, 205, 401, 551, 1749 |
| NaeI | GCCGGC | 1 cut(s) | 100 |
| NarI | GGCGCC | 1 cut(s) | 66 |
| NciI | CCSGG | 1 cut(s) | 2091 |
| NdeI | CATATG | 2 cut(s) | 1119, 1462 |
| NgoMIV | GCCGGC | 1 cut(s) | 98 |
| NmeAIII | GCCGAG | 1 cut(s) | 892 |
| NmuCI | GTSAC | 5 cut(s) | 434, 445, 895, 1333, 2738 |
| NruI | TCGCGA | 1 cut(s) | 401 |
| NsiI | ATGCAT | 2 cut(s) | 1191, 3220 |
| NspI | RCATGY | 7 cut(s) | 520, 967, 1189, 1193, 1203, 3053, 3295 |
| NspV | TTCGAA | 1 cut(s) | 542 |
| PaeI | GCATGC | 2 cut(s) | 1189, 1193 |
| PagI | TCATGA | 1 cut(s) | 2766 |
| PaqCI | CACCTGC | 1 cut(s) | 2073 |
| PceI | AGGCCT | 1 cut(s) | 2791 |
| PciI | ACATGT | 2 cut(s) | 963, 3049 |
| PciSI | GCTCTTC | 1 cut(s) | 3558 |
| PcsI | WCGNNNNNNNCGW | 7 cut(s) | 141, 264, 405, 435, 548, 758, 2602 |
| PctI | GAATGC | 1 cut(s) | 423 |
| PdiI | GCCGGC | 1 cut(s) | 100 |
| PfeI | GAWTC | 7 cut(s) | 649, 1175, 1871, 1883, 2012, 2354, 2836 |
| PflFI | GACNNNGTC | 2 cut(s) | 230, 683 |
| PinAI | ACCGGT | 2 cut(s) | 2309, 2875 |
| PleI | GAGTC | 7 cut(s) | 368, 442, 565, 600, 2046, 2275, 3379 |
| PluTI | GGCGCC | 1 cut(s) | 69 |
| PmaCI | CACGTG | 1 cut(s) | 434 |
| PmlI | CACGTG | 1 cut(s) | 434 |
| PpsI | GAGTC | 7 cut(s) | 368, 442, 565, 600, 2046, 2275, 3379 |
| Ppu21I | YACGTR | 2 cut(s) | 434, 1718 |
| PpuMI | RGGWCCY | 1 cut(s) | 3503 |
| PscI | ACATGT | 2 cut(s) | 963, 3049 |
| Psp5II | RGGWCCY | 1 cut(s) | 3503 |
| Psp6I | CCWGG | 1 cut(s) | 985 |
| PspCI | CACGTG | 1 cut(s) | 434 |
| PspGI | CCWGG | 1 cut(s) | 985 |
| PspPI | GGNCC | 5 cut(s) | 499, 747, 1657, 2898, 3503 |
| PspPPI | RGGWCCY | 1 cut(s) | 3503 |
| PstI | CTGCAG | 3 cut(s) | 2167, 2639, 3532 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1499, 1969 |
| PsuI | RGATCY | 8 cut(s) | 21, 931, 1792, 1819, 2623, 2755, 3244, 3336 |
| PsyI | GACNNNGTC | 2 cut(s) | 230, 683 |
| RruI | TCGCGA | 1 cut(s) | 401 |
| RsaI | GTAC | 5 cut(s) | 971, 2631, 2924, 2932, 3290 |
| RsaNI | GTAC | 5 cut(s) | 970, 2630, 2923, 2931, 3289 |
| SalI | GTCGAC | 1 cut(s) | 3158 |
| SapI | GCTCTTC | 1 cut(s) | 3558 |
| SaqAI | TTAA | 9 cut(s) | 707, 777, 1137, 1326, 1667, 2412, 2504, 2510, 2786 |
| Sau96I | GGNCC | 5 cut(s) | 499, 747, 1657, 2898, 3503 |
| ScaI | AGTACT | 1 cut(s) | 2631 |
| SchI | GAGTC | 7 cut(s) | 368, 442, 566, 600, 2046, 2276, 3379 |
| ScrFI | CCNGG | 2 cut(s) | 987, 2091 |
| SduI | GDGCHC | 7 cut(s) | 912, 1483, 1747, 1773, 1930, 3553, 3570 |
| SfaNI | GCATC | 7 cut(s) | 1294, 1379, 2035, 2660, 2996, 3205, 3227 |
| SfcI | CTRYAG | 4 cut(s) | 2163, 2635, 3248, 3528 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 507 |
| SfoI | GGCGCC | 1 cut(s) | 67 |
| SfuI | TTCGAA | 1 cut(s) | 542 |
| SgrAI | CRCCGGYG | 1 cut(s) | 239 |
| SinI | GGWCC | 2 cut(s) | 1657, 3503 |
| SmlI | CTYRAG | 3 cut(s) | 1165, 2054, 2279 |
| SmoI | CTYRAG | 3 cut(s) | 1165, 2054, 2279 |
| SphI | GCATGC | 2 cut(s) | 1189, 1193 |
| SseBI | AGGCCT | 1 cut(s) | 2791 |
| SspDI | GGCGCC | 1 cut(s) | 65 |
| SspMI | CTAG | 5 cut(s) | 1065, 1901, 1953, 2232, 3138 |
| StuI | AGGCCT | 1 cut(s) | 2791 |
| StyD4I | CCNGG | 2 cut(s) | 985, 2089 |
| StyI | CCWWGG | 2 cut(s) | 1455, 3163 |
| TaaI | ACNGT | 9 cut(s) | 232, 754, 2143, 2450, 2565, 2647, 3064, 3422, 3548 |
| TaiI | ACGT | 4 cut(s) | 279, 436, 1699, 1720 |
| TaqII | GACCGA | 1 cut(s) | 2861 |
| TatI | WGTACW | 4 cut(s) | 969, 2629, 2922, 3288 |
| TauI | GCSGC | 6 cut(s) | 98, 105, 287, 290, 308, 3092 |
| TfiI | GAWTC | 7 cut(s) | 649, 1175, 1871, 1883, 2012, 2354, 2836 |
| Tru1I | TTAA | 9 cut(s) | 707, 777, 1137, 1326, 1667, 2412, 2504, 2510, 2786 |
| Tru9I | TTAA | 9 cut(s) | 707, 777, 1137, 1326, 1667, 2412, 2504, 2510, 2786 |
| TseFI | GTSAC | 5 cut(s) | 434, 445, 895, 1333, 2738 |
| Tsp45I | GTSAC | 5 cut(s) | 434, 445, 895, 1333, 2738 |
| TspGWI | ACGGA | 4 cut(s) | 103, 787, 2114, 2829 |
| Tth111I | GACNNNGTC | 2 cut(s) | 230, 683 |
| VneI | GTGCAC | 1 cut(s) | 1479 |
| VpaK11BI | GGWCC | 2 cut(s) | 1657, 3503 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1351 |
| XapI | RAATTY | 5 cut(s) | 637, 1662, 2491, 2882, 3194 |
| XbaI | TCTAGA | 1 cut(s) | 1900 |
| XceI | RCATGY | 7 cut(s) | 520, 967, 1189, 1193, 1203, 3053, 3295 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 1536, 2082 |
| XmiI | GTMKAC | 4 cut(s) | 1714, 2270, 3159, 3581 |
| XspI | CTAG | 5 cut(s) | 1065, 1901, 1953, 2232, 3138 |
| ZraI | GACGTC | 1 cut(s) | 277 |
| ZrmI | AGTACT | 1 cut(s) | 2631 |
| Zsp2I | ATGCAT | 2 cut(s) | 1191, 3220 |