MD06G1184100.v1.1

TPLATE-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0011200
Physical Location & Seq
Forward (+)
32233437 .. 32240544
7108 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1184100.v1.1.491

Sequence Viewer

Length: 3510 bp
ATGGACATACTCTTCGCCCAGATCCAAGCGGACCTCCGGTCCAACGACGCCCTCCGCCAGTCCGGCGCTCTACTCCAAGCCCTCCAGCAATCCGCCGCCGGCCGCGACATCTCCGTCATCGCCAAGACCGCTGTCGAAGAGATCGTCGCCTCCCCCGCCTCCGCCGTCAGCAAAAAACTCGCCTTTGACCTCATTCGCTCCACCCGCCTCACCGCCGACCTCTGGGACACCGTCTGCGTCGGAATTCTCACCGATCTCGACTTCCCCGACCCTGACGTCAGCGCCGCCGCCGTCTCCATCCTCGCCGCAATCCCTTCCTACCGCCTCTCCAAGCTCATTACCGACGCGCAGAAAGAAATCAACAGTTGCTTCGATTCGCCGAGCGACAATCTCCGCTTCTCCATTACTGAAACCCTCGGATGCATTCTGGCGCGCGATGACCTCGTCACGCTCTGCGAGAACAACGTCAATTTGCTCGACAAGGTGTCGAATTGGTGGTCCCGCATTGGCCAGAACATGCTCGACAGATCCGACGCCGTTTCAAAGGTCGCGTTCGAGTCGGTGGGCCGGTTGTTTCAGGAGTTCGATTCCAAGAGGATGAGCAGACTTGCCGGTGACAAGTTAGTGGATAGTGAGAACTCCCTGGCGATTCGATCCAATTGGGTGTCGTCCATGGTCGACTTCGTGTGGAAGAAGCGGAGTGCTTTAATGGCGAGGTCGTTAGTTTTGCCGGTGGAGAGTTTCCGGGCCACTGTGTTTCCTATCGTTTATGCGGTCAAGGCCATTGCTTCGGGCTCAGTGGAGGTCATAAGGAAGCTGTCAAAGTCGTCTAAGGGGTCCAGTGGGACGGTTGTGGATACCAATGCGGAGAGGTTGGTGGGAGTTTCAGATGTGGTCACGCATTTGGTGCCATTCTTGGCATCGTCATTGGATCCGGCTTTGATTTTTGAAGTTGGGATTGATATGCTGTATCTGGCTGATGTGCCTGGTGGGAAACCCGAGTGGGCTTCGCAGTCGATTATCGCAATTCTCACACTTTGGGATAGGCAAGAGTTTGCTTCTGCAAGAGAGAGTATTGTTAGAGCTGTGGTTACTAACCTGCACCTGCTTGATCTTCATATGCAGGTTTCACTGTTTAAGAGGTTGCTTCTTATGGTGAGAAACTTGAGAGCAGAATCAGATCGTATGCATGCGTTAGCTTGTATTTGTCGAACAGCTCTTTGTGTTGATCTCTTCGCAAAGGAGAGTGTAAGAAGAGGCCAGAAACCTCTTGCCGGAACTGATATAGCTTCACTTTTTGAGGACGCAAGAATCAAAGACGATCTTAATAGTGTGACAAGCAAGACCTTATTTAGGGAGGAGTTAGTGGCATCATTAGTTGAAAGTTGTTTTCAGTTGTCTCTGCCTTTACCTGAACAAAAGAACACAGGGATGGAGAGCAGGGTTATTGGAGCCTTGGCATATGGAACTGGATATGGTGCACTAAATTGGACAGAGCCTGCTTTGGAAGTGGTGGAGGTTTGCCGGCCTTGTGTCAAATGGGATTGTGAGGGCCGGACCTATGCAATTGATTGCTATTTGAAGTTGCTTGTGAGGCTTTGTCATATCTATGATACTAGGGGAGGTGTAAAAAGAGTTAAAGATGGGGCTTCCCAAGACCAAATTTTAAATGAGACAAGGTTGCAGAATTTGCAGCGTGAACTTGTGAAAGATCTACGCGAGGTACATACCCCAAGAATATGTGCTCGCCTTATTTGGGCTATTTCAGAGCACATAGATCTAGAAGGTCTGGACCCACTTCTTGCTGATGATCCCGAGGATCCATTGAACATGATTATTTCGAACATCCACAAGGTTTTGTTCAATATAGATTCATCTGCTGATTCAACAAACAGGCTTCTAGATGTTCAGGCAGTTCTTTTATGTGCTCAGCGGTTGGGATCACGCAACCCCAGGGCCGGGCAGTTGCTGACAAAAGAACTTGAAGAGTTCAGGAGCGGTAGTATGGCGGATTCTGTTAACAAGCATCAGAGCCGTTTGATTTTGCAGAGAATCAAGTATGTTACAAGTCATCCAGAAAGCAGGTGGGCAGGAGTGAGTGAAGCCAGAGGTGACTACCCATTTAGCCACCATAAACTAACTGTCCAGTTCTACGAAGTAGCTGCAGCTCAGGATAGAAAATTAGAAGGATTGGTTCATAAGGCTATTCTAGAGCTTTGGAGGCCAGATCCTAGTGAACTAACCCTTCTGCTGACTAAAGGAGTTGACTCTACTTTACTCAAAGTTCCTCCTAGTGCAATTACTTTGACTGGTAGCAGTGATCCTTGCTACATCGAGGGATATCATTTGGCAGATTCTAGTGATGGAAGGATATCTCTGCACTTGAAGGTCTTAAATTTAACTGAACTTGAGCTGAACCGGGTTGATATTCGAGTTGGGTTATCTGGTGCATTATATTTTATGGATGGGTCTCCTCAAGCAGTACGGCAGTTGCGTAGCCTTGTTTCACAGGATCCAGTACTTTGCAGCGTGACTGTGGGTGTCTCCCATTTTGAAAGGTGCGCCCTTTGGGTTCAAGTCTTATACTACCCCTTTTATGGAAGTGCTGCTATAGATTATGAAGGTGACTACACCGAAGAAGATCCGCAAATCATGAGACAAAAGAGAAGTTTAAGGCCAGAACTGGGAGAACCAGTCATTTTAAGGTGTCAGCCATACAAAATTCCGCTGACCGAGCTTCTTTTGCCTCATAAGATCTCCCCTGTTGAATTTTTCCGTCTTTGGCCCAGTTTACCGGCCATAGTAGAGTACACTGGTACATACACTTATGAAGGAAGTGGCTTCAAGGCTACTGCTGCACAGCAGTATGGGGCATCTCCTTTCCTCAGTGGGCTAAAATCCTTGTCATCCAAGCCATTCCACAGAGTTTGTTCGCATATTATCCGAACAGTGGCAGGATTTCAGTTGTGTTTTGCTGCAAAAACCTGGTATGGTGGCTTCTTGGGCCTGATGATCTTTGGAGCTAGTGAAGTGAGCCGGAATGTTGACCTAGGTGATGAGACTACGACCATGATATGCAAATTTGTGGTTCGAGCATCTGATGCATCAATTACAAAGGAGATCGGATCGGATCTACAGGGCTGGTTGGATGACCTCACTGATGGGGGTGTTGAGTACATGCCTGAAGACGAAGTGAAGGTGGCTGCTGCGGAGAGGCTTAGGATCTCGATGGAACGGATAGCCTTGCTAAAGGCAGCCCAACCTAAGAGAAAGATTCCCAAATCTGATGATGAAGAAGACGAAGAAGAAGATGAAAGCGACGAAGAAGATGAGGACAAAGTCAAGAAAAACAAGGAAAAGAAAAAAGATGGCGAGGAAAATGGAAAACCAAAGGGACCTACAACCCTGTCGAAGTTGACAGCAGAGGAGGCCGAACACCGTGCTCTTCAAACAGCAGTGCTCCAAGAGTGGCATACGTTGTGCAAGGATAGAAGTGCTAAAGTTAATTGA

Protein Analysis

1170

Amino Acids

129.86

Weight (kDa)

5.57

Isoelectric Point (pI)

44.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011200)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G01780 AT3G01780
fragaria_vesca FvH4_5g15940
malus_domestica MD06G1184100.v1.1 MD14G1190000.v1.1
prunus_persica Prupe.5G183000_v2.0.a1
pyrus_communis pycom06g16320 pycom06g16330 pycom14g15780
rosa_chinensis RchiOBHm_Chr7g0180111
rosa_laevigata RLG00000005292
rosa_multiflora Rmu_sc0017616.1_g000007 Rmu_ssc0000119.1_g000034
rosa_roxburghii Rroxscaffold_3G00273120
rosa_rugosa Rorug06G0430700
rosa_samantha Rh7AG031100 Rh7BG031000 Rh7CG033000 Rh7DG031600
rosa_wichuraiana Rw7G002580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1113, 2073
AasI GACNNNNNNGTC 2 cut(s) 113, 275
AatII GACGTC 1 cut(s) 279
Acc36I ACCTGC 4 cut(s) 1107, 1113, 1114, 2073
AccB1I GGYRCC 1 cut(s) 907
AccBSI CCGCTC 1 cut(s) 1998
AccI GTMKAC 1 cut(s) 678
AccII CGCG 6 cut(s) 105, 347, 433, 435, 551, 1719
AcoI YGGCCR 3 cut(s) 100, 508, 2796
AcsI RAATTY 7 cut(s) 243, 1662, 1687, 2395, 2721, 2768, 3080
AcuI CTGAAG 1 cut(s) 3204
AcyI GRCGYC 3 cut(s) 48, 276, 534
AfaI GTAC 6 cut(s) 1725, 2484, 2520, 2810, 2818, 3176
AfiI CCNNNNNNNGG 9 cut(s) 222, 1274, 1353, 1756, 1958, 1959, 2597, 2684, 2950
AhdI GACNNNNNGTC 2 cut(s) 37, 484
AjnI CCWGG 4 cut(s) 642, 985, 1952, 2984
Alw21I GWGCWC 6 cut(s) 1483, 1747, 1773, 1930, 3445, 3462
Alw26I GTCTC 7 cut(s) 298, 1404, 1667, 2475, 2548, 2650, 3053
Alw44I GTGCAC 1 cut(s) 1479
AlwNI CAGNNNCTG 2 cut(s) 1499, 1969
Ama87I CYCGRG 2 cut(s) 998, 1814
ApaLI GTGCAC 1 cut(s) 1479
ApoI RAATTY 7 cut(s) 243, 1662, 1687, 2395, 2721, 2768, 3080
ArsI GACNNNNNNTTYG 5 cut(s) 28, 129, 161, 482, 514
AspA2I CCTAGG 1 cut(s) 3049
AspLEI GCGC 6 cut(s) 68, 284, 349, 433, 435, 2564
AsuC2I CCSGG 3 cut(s) 746, 1960, 2420
AsuHPI GGTGA 7 cut(s) 202, 241, 626, 1168, 2123, 2636, 3065
AsuII TTCGAA 1 cut(s) 1841
AvaI CYCGRG 2 cut(s) 998, 1814
AvaII GGWCC 7 cut(s) 31, 39, 498, 837, 1557, 1792, 3395
AvrII CCTAGG 1 cut(s) 3049
BaeGI GKGCMC 1 cut(s) 1483
BalI TGGCCA 1 cut(s) 510
BamHI GGATCC 3 cut(s) 931, 1819, 2512
BanI GGYRCC 1 cut(s) 907
BanII GRGCYC 1 cut(s) 797
BarI GAAGNNNNNNTAC 2 cut(s) 2981, 3013
BbsI GAAGAC 2 cut(s) 3192, 3303
Bbv12I GWGCWC 6 cut(s) 1483, 1747, 1773, 1930, 3445, 3462
BccI CCATC 8 cut(s) 305, 1426, 1637, 2357, 2459, 3155, 3223, 3362
BceAI ACGGC 5 cut(s) 149, 275, 521, 2019, 2501
BcgI CGANNNNNNTGC 6 cut(s) 160, 194, 2144, 2178, 3422, 3456
BciT130I CCWGG 4 cut(s) 644, 987, 1954, 2986
BciVI GTATCC 1 cut(s) 850
BcnI CCSGG 3 cut(s) 746, 1960, 2420
BcoDI GTCTC 7 cut(s) 298, 1404, 1667, 2475, 2548, 2650, 3053
BfaI CTAG 9 cut(s) 1617, 1781, 1901, 2210, 2232, 2292, 2358, 3024, 3050
BfmI CTRYAG 3 cut(s) 2163, 2610, 3134
BfoI RGCGCY 2 cut(s) 69, 285
BfuAI ACCTGC 4 cut(s) 1107, 1113, 1114, 2073
BfuI GTATCC 1 cut(s) 850
BglI GCCNNNNNGGC 1 cut(s) 63
BglII AGATCT 3 cut(s) 1711, 1777, 2754
BlnI CCTAGG 1 cut(s) 3049
BlpI GCTNAGC 1 cut(s) 1929
BmcAI AGTACT 1 cut(s) 2520
Bme1390I CCNGG 7 cut(s) 644, 746, 987, 1954, 1960, 2420, 2986
Bme18I GGWCC 7 cut(s) 31, 39, 498, 837, 1557, 1792, 3395
BmeRI GACNNNNNGTC 2 cut(s) 37, 484
BmeT110I CYCGRG 2 cut(s) 998, 1814
BmiI GGNNCC 9 cut(s) 500, 838, 909, 933, 1453, 1794, 1821, 2514, 3396
BmrFI CCNGG 7 cut(s) 644, 746, 987, 1954, 1960, 2420, 2986
BmrI ACTGGG 2 cut(s) 2693, 2781
BmsI GCATC 8 cut(s) 410, 929, 1379, 2035, 2882, 3091, 3104, 3113
BmuI ACTGGG 2 cut(s) 2693, 2781
BoxI GACNNNNGTC 1 cut(s) 131
BpiI GAAGAC 2 cut(s) 3192, 3303
BpmI CTGGAG 1 cut(s) 68
Bpu10I CCTNAGC 2 cut(s) 2169, 3218
Bpu1102I GCTNAGC 1 cut(s) 1929
Bpu14I TTCGAA 1 cut(s) 1841
BpuEI CTTGAG 3 cut(s) 1186, 2429, 2460
BpuMI CCSGG 3 cut(s) 746, 1960, 2420
BsaHI GRCGYC 3 cut(s) 48, 276, 534
BsaI GGTCTC 1 cut(s) 2475
BsaJI CCNNGG 8 cut(s) 415, 642, 672, 1455, 1815, 1952, 1953, 3049
BsaWI WCCGGW 1 cut(s) 36
BsaXI ACNNNNNCTCC 6 cut(s) 311, 341, 1987, 2017, 2679, 2709
Bsc4I CCNNNNNNNGG 9 cut(s) 222, 1274, 1353, 1756, 1958, 1959, 2597, 2684, 2950
Bse118I RCCGGY 6 cut(s) 98, 567, 611, 730, 1524, 2794
Bse3DI GCAATG 1 cut(s) 783
BseBI CCWGG 4 cut(s) 644, 987, 1954, 2986
BseDI CCNNGG 8 cut(s) 415, 642, 672, 1455, 1815, 1952, 1953, 3049
BseGI GGATG 9 cut(s) 297, 425, 603, 1437, 1845, 2071, 2470, 2906, 3154
BseLI CCNNNNNNNGG 9 cut(s) 222, 1274, 1353, 1756, 1958, 1959, 2597, 2684, 2950
BseMI GCAATG 1 cut(s) 783
BseMII CTCAG 4 cut(s) 810, 1943, 2183, 2899
BsePI GCGCGC 1 cut(s) 431
BseRI GAGGAG 3 cut(s) 1373, 2463, 3440
BseSI GKGCMC 1 cut(s) 1483
BseX3I CGGCCG 1 cut(s) 100
BsgI GTGCAG 3 cut(s) 1085, 2363, 2841
Bsh1236I CGCG 6 cut(s) 105, 347, 433, 435, 551, 1719
Bsh1285I CGRYCG 1 cut(s) 103
BshNI GGYRCC 1 cut(s) 907
BsiEI CGRYCG 1 cut(s) 103
BsiHKAI GWGCWC 6 cut(s) 1483, 1747, 1773, 1930, 3445, 3462
BsiHKCI CYCGRG 2 cut(s) 998, 1814
BslFI GGGAC 4 cut(s) 239, 484, 859, 3408
BslI CCNNNNNNNGG 9 cut(s) 222, 1274, 1353, 1756, 1958, 1959, 2597, 2684, 2950
BsmAI GTCTC 7 cut(s) 298, 1404, 1667, 2475, 2548, 2650, 3053
BsmBI CGTCTC 1 cut(s) 298
BsmFI GGGAC 4 cut(s) 239, 484, 859, 3408
BsmI GAATGC 1 cut(s) 423
Bso31I GGTCTC 1 cut(s) 2475
BsoBI CYCGRG 2 cut(s) 998, 1814
Bsp119I TTCGAA 1 cut(s) 1841
Bsp1286I GDGCHC 7 cut(s) 797, 1483, 1747, 1773, 1930, 3445, 3462
Bsp1720I GCTNAGC 1 cut(s) 1929
Bsp19I CCATGG 1 cut(s) 672
BspCNI CTCAG 4 cut(s) 809, 1942, 2182, 2898
BspFNI CGCG 6 cut(s) 105, 347, 433, 435, 551, 1719
BspHI TCATGA 1 cut(s) 2652
BspLI GGNNCC 9 cut(s) 500, 838, 909, 933, 1453, 1794, 1821, 2514, 3396
BspMAI CTGCAG 1 cut(s) 2167
BspMI ACCTGC 4 cut(s) 1107, 1113, 1114, 2073
BspQI GCTCTTC 1 cut(s) 3450
BspT104I TTCGAA 1 cut(s) 1841
BspT107I GGYRCC 1 cut(s) 907
BspTNI GGTCTC 1 cut(s) 2475
BsrBI CCGCTC 1 cut(s) 1998
BsrDI GCAATG 1 cut(s) 783
BsrFI RCCGGY 6 cut(s) 98, 567, 611, 730, 1524, 2794
BssAI RCCGGY 6 cut(s) 98, 567, 611, 730, 1524, 2794
BssECI CCNNGG 8 cut(s) 415, 642, 672, 1455, 1815, 1952, 1953, 3049
BssHII GCGCGC 1 cut(s) 431
BssNI GRCGYC 3 cut(s) 48, 276, 534
BssT1I CCWWGG 3 cut(s) 672, 1455, 3049
Bst2UI CCWGG 4 cut(s) 644, 987, 1954, 2986
Bst4CI ACNGT 9 cut(s) 232, 365, 754, 850, 1134, 2143, 2536, 2950, 3440
Bst6I CTCTTC 6 cut(s) 17, 132, 1238, 1249, 1980, 3450
BstACI GRCGYC 3 cut(s) 48, 276, 534
BstAPI GCANNNNNTGC 3 cut(s) 907, 1690, 3101
BstBI TTCGAA 1 cut(s) 1841
BstC8I GCNNGC 6 cut(s) 100, 433, 1191, 1500, 1526, 1747
BstDEI CTNAG 7 cut(s) 796, 831, 1929, 2169, 2885, 3218, 3264
BstDSI CCRYGG 1 cut(s) 672
BstENI CCTNNNNNAGG 1 cut(s) 1351
BstF5I GGATG 9 cut(s) 297, 425, 603, 1437, 1845, 2071, 2470, 2906, 3154
BstFNI CGCG 6 cut(s) 105, 347, 433, 435, 551, 1719
BstH2I RGCGCY 2 cut(s) 69, 285
BstHHI GCGC 6 cut(s) 68, 284, 349, 433, 435, 2564
BstMAI GTCTC 7 cut(s) 298, 1404, 1667, 2475, 2548, 2650, 3053
BstMCI CGRYCG 1 cut(s) 103
BstNI CCWGG 4 cut(s) 644, 987, 1954, 2986
BstNSI RCATGY 3 cut(s) 520, 1193, 3181
BstPAI GACNNNNGTC 1 cut(s) 131
BstSCI CCNGG 7 cut(s) 642, 744, 985, 1952, 1958, 2418, 2984
BstSFI CTRYAG 3 cut(s) 2163, 2610, 3134
BstSLI GKGCMC 1 cut(s) 1483
BstUI CGCG 6 cut(s) 105, 347, 433, 435, 551, 1719
BstV2I GAAGAC 2 cut(s) 3192, 3303
BstZI CGGCCG 1 cut(s) 100
BsuI GTATCC 1 cut(s) 850
BtgI CCRYGG 1 cut(s) 672
BtgZI GCGATG 2 cut(s) 103, 450
BtsCI GGATG 9 cut(s) 297, 425, 603, 1437, 1845, 2071, 2470, 2906, 3154
BtsI GCAGTG 2 cut(s) 2323, 3462
BveI ACCTGC 4 cut(s) 1107, 1113, 1114, 2073
Cac8I GCNNGC 6 cut(s) 100, 433, 1191, 1500, 1526, 1747
CaiI CAGNNNCTG 2 cut(s) 1499, 1969
CciI TCATGA 1 cut(s) 2652
CfoI GCGC 6 cut(s) 68, 284, 349, 433, 435, 2564
Cfr10I RCCGGY 6 cut(s) 98, 567, 611, 730, 1524, 2794
CseI GACGC 5 cut(s) 56, 226, 353, 542, 1313
CsiI ACCWGGT 1 cut(s) 2984
Csp6I GTAC 6 cut(s) 1724, 2483, 2519, 2809, 2817, 3175
CspCI CAANNNNNGTGG 2 cut(s) 2909, 2944
CviAII CATG 7 cut(s) 517, 673, 1190, 1831, 2653, 3070, 3178
CviQI GTAC 6 cut(s) 1724, 2483, 2519, 2809, 2817, 3175
DdeI CTNAG 7 cut(s) 796, 831, 1929, 2169, 2885, 3218, 3264
DraI TTTAAA 1 cut(s) 1668
DrdI GACNNNNNNGTC 2 cut(s) 113, 275
DriI GACNNNNNGTC 2 cut(s) 37, 484
DseDI GACNNNNNNGTC 2 cut(s) 113, 275
EaeI YGGCCR 3 cut(s) 100, 508, 2796
EagI CGGCCG 1 cut(s) 100
Eam1104I CTCTTC 6 cut(s) 17, 132, 1238, 1249, 1980, 3450
Eam1105I GACNNNNNGTC 2 cut(s) 37, 484
EarI CTCTTC 6 cut(s) 17, 132, 1238, 1249, 1980, 3450
EciI GGCGGA 4 cut(s) 44, 82, 151, 2024
EclXI CGGCCG 1 cut(s) 100
Eco130I CCWWGG 3 cut(s) 672, 1455, 3049
Eco24I GRGCYC 1 cut(s) 797
Eco31I GGTCTC 1 cut(s) 2475
Eco32I GATATC 2 cut(s) 2342, 2373
Eco47I GGWCC 7 cut(s) 31, 39, 498, 837, 1557, 1792, 3395
Eco52I CGGCCG 1 cut(s) 100
Eco57I CTGAAG 1 cut(s) 3204
Eco88I CYCGRG 2 cut(s) 998, 1814
EcoNI CCTNNNNNAGG 1 cut(s) 1351
EcoO109I RGGNCCY 1 cut(s) 3395
EcoRI GAATTC 1 cut(s) 243
EcoRII CCWGG 4 cut(s) 642, 985, 1952, 2984
EcoRV GATATC 2 cut(s) 2342, 2373
EcoT14I CCWWGG 3 cut(s) 672, 1455, 3049
EcoT22I ATGCAT 3 cut(s) 425, 1191, 3106
EcoT38I GRGCYC 1 cut(s) 797
ErhI CCWWGG 3 cut(s) 672, 1455, 3049
Esp3I CGTCTC 1 cut(s) 298
FaeI CATG 7 cut(s) 520, 676, 1193, 1834, 2656, 3073, 3181
FalI AAGNNNNNCTT 2 cut(s) 1308, 1340
FaqI GGGAC 4 cut(s) 239, 484, 859, 3408
FatI CATG 7 cut(s) 516, 672, 1189, 1830, 2652, 3069, 3177
FauI CCCGC 3 cut(s) 163, 212, 509
FauNDI CATATG 2 cut(s) 1119, 1462
FblI GTMKAC 1 cut(s) 678
FokI GGATG 9 cut(s) 284, 432, 610, 1444, 1832, 2058, 2477, 2893, 3161
FriOI GRGCYC 1 cut(s) 797
FspBI CTAG 9 cut(s) 1617, 1781, 1901, 2210, 2232, 2292, 2358, 3024, 3050
GlaI GCGC 6 cut(s) 67, 283, 348, 432, 434, 2563
GsuI CTGGAG 1 cut(s) 68
HaeII RGCGCY 2 cut(s) 69, 285
HgaI GACGC 5 cut(s) 56, 226, 353, 542, 1313
HhaI GCGC 6 cut(s) 68, 284, 349, 433, 435, 2564
Hin1I GRCGYC 3 cut(s) 48, 276, 534
Hin1II CATG 7 cut(s) 520, 676, 1193, 1834, 2656, 3073, 3181
Hin6I GCGC 6 cut(s) 66, 282, 347, 431, 433, 2562
HinP1I GCGC 6 cut(s) 66, 282, 347, 431, 433, 2562
HincII GTYRAC 5 cut(s) 679, 2020, 2266, 3046, 3417
HindII GTYRAC 5 cut(s) 679, 2020, 2266, 3046, 3417
HpaI GTTAAC 1 cut(s) 2020
HphI GGTGA 7 cut(s) 202, 241, 626, 1168, 2123, 2636, 3065
Hpy99I CGWCG 6 cut(s) 50, 149, 242, 347, 536, 3323
HpyAV CCTTC 9 cut(s) 324, 1778, 2180, 2255, 2361, 2380, 2615, 2825, 3190
HpyCH4III ACNGT 9 cut(s) 232, 365, 754, 850, 1134, 2143, 2536, 2950, 3440
HpyCH4IV ACGT 3 cut(s) 276, 465, 3476
HpyF3I CTNAG 7 cut(s) 796, 831, 1929, 2169, 2885, 3218, 3264
HpySE526I ACGT 3 cut(s) 276, 465, 3476
Hsp92I GRCGYC 3 cut(s) 48, 276, 534
Hsp92II CATG 7 cut(s) 520, 676, 1193, 1834, 2656, 3073, 3181
HspAI GCGC 6 cut(s) 66, 282, 347, 431, 433, 2562
KroI GCCGGC 2 cut(s) 98, 1524
KroNI GCCGGC 2 cut(s) 100, 1526
KspAI GTTAAC 1 cut(s) 2020
LguI GCTCTTC 1 cut(s) 3450
LmnI GCTCC 5 cut(s) 203, 1451, 1995, 3020, 3465
LweI GCATC 8 cut(s) 410, 929, 1379, 2035, 2882, 3091, 3104, 3113
MabI ACCWGGT 1 cut(s) 2984
MaeI CTAG 9 cut(s) 1617, 1781, 1901, 2210, 2232, 2292, 2358, 3024, 3050
MaeII ACGT 3 cut(s) 276, 465, 3476
MaeIII GTNAC 9 cut(s) 445, 614, 895, 1090, 1333, 2062, 2111, 2530, 2624
MbiI CCGCTC 1 cut(s) 1998
MfeI CAATTG 2 cut(s) 658, 1566
MhlI GDGCHC 7 cut(s) 797, 1483, 1747, 1773, 1930, 3445, 3462
MlsI TGGCCA 1 cut(s) 510
MluNI TGGCCA 1 cut(s) 510
MlyI GAGTC 2 cut(s) 566, 2261
MmeI TCCRAC 4 cut(s) 66, 220, 555, 3126
Mox20I TGGCCA 1 cut(s) 510
Mph1103I ATGCAT 3 cut(s) 425, 1191, 3106
MroNI GCCGGC 2 cut(s) 98, 1524
MscI TGGCCA 1 cut(s) 510
MslI CAYNNNNRTG 2 cut(s) 1430, 1608
Msp20I TGGCCA 1 cut(s) 510
MspA1I CMGCKG 3 cut(s) 131, 1933, 2728
MspR9I CCNGG 7 cut(s) 644, 746, 987, 1954, 1960, 2420, 2986
MunI CAATTG 2 cut(s) 658, 1566
Mva1269I GAATGC 1 cut(s) 423
MvaI CCWGG 4 cut(s) 644, 987, 1954, 2986
MvnI CGCG 6 cut(s) 105, 347, 433, 435, 551, 1719
NaeI GCCGGC 2 cut(s) 100, 1526
NciI CCSGG 3 cut(s) 746, 1960, 2420
NcoI CCATGG 1 cut(s) 672
NdeI CATATG 2 cut(s) 1119, 1462
NgoMIV GCCGGC 2 cut(s) 98, 1524
NlaIII CATG 7 cut(s) 520, 676, 1193, 1834, 2656, 3073, 3181
NlaIV GGNNCC 9 cut(s) 500, 838, 909, 933, 1453, 1794, 1821, 2514, 3396
NmeAIII GCCGAG 1 cut(s) 405
NmuCI GTSAC 7 cut(s) 445, 614, 895, 1333, 2111, 2530, 2624
NsiI ATGCAT 3 cut(s) 425, 1191, 3106
NspI RCATGY 3 cut(s) 520, 1193, 3181
NspV TTCGAA 1 cut(s) 1841
PaeI GCATGC 1 cut(s) 1193
PagI TCATGA 1 cut(s) 2652
PaqCI CACCTGC 2 cut(s) 1113, 2073
PasI CCCWGGG 1 cut(s) 1953
PauI GCGCGC 1 cut(s) 431
PciSI GCTCTTC 1 cut(s) 3450
PcsI WCGNNNNNNNCGW 4 cut(s) 141, 264, 528, 2491
PctI GAATGC 1 cut(s) 423
PdiI GCCGGC 2 cut(s) 100, 1526
PflFI GACNNNGTC 3 cut(s) 230, 443, 3338
PleI GAGTC 2 cut(s) 565, 2261
PpsI GAGTC 2 cut(s) 565, 2261
PpuMI RGGWCCY 1 cut(s) 3395
PshAI GACNNNNGTC 1 cut(s) 131
Psp5II RGGWCCY 1 cut(s) 3395
Psp6I CCWGG 4 cut(s) 642, 985, 1952, 2984
PspGI CCWGG 4 cut(s) 642, 985, 1952, 2984
PspN4I GGNNCC 9 cut(s) 500, 838, 909, 933, 1453, 1794, 1821, 2514, 3396
PspPPI RGGWCCY 1 cut(s) 3395
PstI CTGCAG 1 cut(s) 2167
PstNI CAGNNNCTG 2 cut(s) 1499, 1969
PsyI GACNNNGTC 3 cut(s) 230, 443, 3338
PteI GCGCGC 1 cut(s) 431
RsaI GTAC 6 cut(s) 1725, 2484, 2520, 2810, 2818, 3176
RsaNI GTAC 6 cut(s) 1724, 2483, 2519, 2809, 2817, 3175
RseI CAYNNNNRTG 2 cut(s) 1430, 1608
SalI GTCGAC 1 cut(s) 677
SapI GCTCTTC 1 cut(s) 3450
ScaI AGTACT 1 cut(s) 2520
SchI GAGTC 2 cut(s) 566, 2261
ScrFI CCNGG 7 cut(s) 644, 746, 987, 1954, 1960, 2420, 2986
SduI GDGCHC 7 cut(s) 797, 1483, 1747, 1773, 1930, 3445, 3462
SexAI ACCWGGT 1 cut(s) 2984
SfaNI GCATC 8 cut(s) 410, 929, 1379, 2035, 2882, 3091, 3104, 3113
SfcI CTRYAG 3 cut(s) 2163, 2610, 3134
SfuI TTCGAA 1 cut(s) 1841
SinI GGWCC 7 cut(s) 31, 39, 498, 837, 1557, 1792, 3395
SmiMI CAYNNNNRTG 2 cut(s) 1430, 1608
SmlI CTYRAG 3 cut(s) 1165, 2408, 2475
SmoI CTYRAG 3 cut(s) 1165, 2408, 2475
SphI GCATGC 1 cut(s) 1193
SspMI CTAG 9 cut(s) 1617, 1781, 1901, 2210, 2232, 2292, 2358, 3024, 3050
StyD4I CCNGG 7 cut(s) 642, 744, 985, 1952, 1958, 2418, 2984
StyI CCWWGG 3 cut(s) 672, 1455, 3049
TaaI ACNGT 9 cut(s) 232, 365, 754, 850, 1134, 2143, 2536, 2950, 3440
TaiI ACGT 3 cut(s) 279, 468, 3479
TaqII GACCGA 1 cut(s) 2747
TatI WGTACW 3 cut(s) 2518, 2808, 3174
TauI GCSGC 5 cut(s) 98, 105, 287, 290, 308
TseFI GTSAC 7 cut(s) 445, 614, 895, 1333, 2111, 2530, 2624
Tsp45I GTSAC 7 cut(s) 445, 614, 895, 1333, 2111, 2530, 2624
TspDTI ATGAA 7 cut(s) 1106, 1863, 2186, 2634, 2844, 3306, 3327
TspGWI ACGGA 3 cut(s) 103, 2765, 3250
Tth111I GACNNNGTC 3 cut(s) 230, 443, 3338
VneI GTGCAC 1 cut(s) 1479
VpaK11BI GGWCC 7 cut(s) 31, 39, 498, 837, 1557, 1792, 3395
XagI CCTNNNNNAGG 1 cut(s) 1351
XapI RAATTY 7 cut(s) 243, 1662, 1687, 2395, 2721, 2768, 3080
XbaI TCTAGA 3 cut(s) 1780, 1900, 2209
XceI RCATGY 3 cut(s) 520, 1193, 3181
XcmI CCANNNNNNNNNTGG 1 cut(s) 2082
XmaJI CCTAGG 1 cut(s) 3049
XmiI GTMKAC 1 cut(s) 678
XspI CTAG 9 cut(s) 1617, 1781, 1901, 2210, 2232, 2292, 2358, 3024, 3050
ZraI GACGTC 1 cut(s) 277
ZrmI AGTACT 1 cut(s) 2520
Zsp2I ATGCAT 3 cut(s) 425, 1191, 3106
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.