FvH4_5g17720

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0011123
Physical Location & Seq
Forward (+)
10152125 .. 10153560
1436 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g17720.t1

Sequence Viewer

Length: 390 bp
ATGGCGTTGCAGTGGATGATACTGACGTATGTGGTGGCGGCAGAGGCGGCGATAGCGCTTCTCCTGACTCTCCCGGCGCCGAAGCTGTTGAAGTTTCGCTTCGTGTCTTTGATCTCTCTCATTCTCCAGCCGGCGCTCTTCGTCGTCCCCTTCGCCGGATTCCAGCTCCTCGATATTTACTGGAAGATGGAGCATCGTTTGACGTGCACCTCAGACATCTGCACTGCTGCTGAGCGAGACCGTTCTGAGAAATCCCTCTACAAGGCCCAAAGGAATGTAATTCTCTGCTCTGCAGCATGTCTTCTTTACTGGTGTATCTATCGTATCTGTAAGTACTACAAGGATATTGAAAGCTTGGAGGAAGTGGAGAAGAGGTTCAAGGAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bap31

Protein Analysis

130

Amino Acids

15.02

Weight (kDa)

8.2

Isoelectric Point (pI)

57.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bap31 PF05529 1 - 121 4.4e-07 Bap31/Bap29 transmembrane region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 76
AciI CCGC 2 cut(s) 38, 47
AcyI GRCGYC 1 cut(s) 77
AfaI GTAC 1 cut(s) 335
AfeI AGCGCT 1 cut(s) 57
AfiI CCNNNNNNNGG 2 cut(s) 155, 262
AgsI TTSAA 3 cut(s) 91, 350, 379
AjiI CACGTC 1 cut(s) 204
AloI GAACNNNNNNTCC 1 cut(s) 359
AluBI AGCT 3 cut(s) 85, 166, 354
AluI AGCT 3 cut(s) 85, 166, 354
Alw21I GWGCWC 1 cut(s) 209
Alw26I GTCTC 1 cut(s) 231
Alw44I GTGCAC 1 cut(s) 205
Aor51HI AGCGCT 1 cut(s) 57
AoxI GGCC 1 cut(s) 264
ApaLI GTGCAC 1 cut(s) 205
ApeKI GCWGC 2 cut(s) 227, 293
Asp700I GAANNNNTTC 1 cut(s) 374
AspLEI GCGC 3 cut(s) 58, 79, 136
AspS9I GGNCC 1 cut(s) 265
AsuC2I CCSGG 1 cut(s) 74
BaeGI GKGCMC 1 cut(s) 209
BaeI ACNNNNGTAYC 4 cut(s) 11, 44, 298, 331
BanI GGYRCC 1 cut(s) 76
BbsI GAAGAC 1 cut(s) 293
Bbv12I GWGCWC 1 cut(s) 209
BbvI GCAGC 2 cut(s) 214, 305
BccI CCATC 1 cut(s) 181
BcnI CCSGG 1 cut(s) 74
BcoDI GTCTC 1 cut(s) 231
BfmI CTRYAG 1 cut(s) 291
BfoI RGCGCY 3 cut(s) 59, 80, 137
BisI GCNGC 4 cut(s) 39, 48, 228, 294
BlpI GCTNAGC 1 cut(s) 231
BlsI GCNGC 4 cut(s) 40, 49, 229, 295
BmcAI AGTACT 1 cut(s) 335
Bme1390I CCNGG 1 cut(s) 74
BmgBI CACGTC 1 cut(s) 204
BmgT120I GGNCC 1 cut(s) 265
BmiI GGNNCC 1 cut(s) 78
BmrFI CCNGG 1 cut(s) 74
BmsI GCATC 1 cut(s) 202
BpiI GAAGAC 1 cut(s) 293
BpmI CTGGAG 1 cut(s) 110
Bpu1102I GCTNAGC 1 cut(s) 231
BpuMI CCSGG 1 cut(s) 74
BsaHI GRCGYC 1 cut(s) 77
BsaI GGTCTC 1 cut(s) 231
BsaXI ACNNNNNCTCC 2 cut(s) 359, 389
Bsc4I CCNNNNNNNGG 2 cut(s) 155, 262
Bse118I RCCGGY 1 cut(s) 130
Bse1I ACTGG 2 cut(s) 185, 314
BseGI GGATG 1 cut(s) 21
BseLI CCNNNNNNNGG 2 cut(s) 155, 262
BseMII CTCAG 3 cut(s) 222, 225, 237
BseNI ACTGG 2 cut(s) 185, 314
BseRI GAGGAG 1 cut(s) 158
BseSI GKGCMC 1 cut(s) 209
BseXI GCAGC 2 cut(s) 214, 305
BsgI GTGCAG 1 cut(s) 205
BshFI GGCC 1 cut(s) 266
BshNI GGYRCC 1 cut(s) 76
BsiHKAI GWGCWC 1 cut(s) 209
BsiSI CCGG 3 cut(s) 74, 131, 156
BslFI GGGAC 1 cut(s) 131
BslI CCNNNNNNNGG 2 cut(s) 155, 262
BsmAI GTCTC 1 cut(s) 231
BsmFI GGGAC 1 cut(s) 131
BsnI GGCC 1 cut(s) 266
Bso31I GGTCTC 1 cut(s) 231
Bsp1286I GDGCHC 1 cut(s) 209
Bsp143I GATC 1 cut(s) 111
Bsp1720I GCTNAGC 1 cut(s) 231
BspACI CCGC 2 cut(s) 38, 47
BspANI GGCC 1 cut(s) 266
BspCNI CTCAG 3 cut(s) 223, 224, 238
BspLI GGNNCC 1 cut(s) 78
BspMAI CTGCAG 1 cut(s) 295
BspQI GCTCTTC 1 cut(s) 143
BspT107I GGYRCC 1 cut(s) 76
BspTNI GGTCTC 1 cut(s) 231
BsrFI RCCGGY 1 cut(s) 130
BsrI ACTGG 2 cut(s) 185, 314
BssAI RCCGGY 1 cut(s) 130
BssMI GATC 1 cut(s) 111
BssNI GRCGYC 1 cut(s) 77
Bst4CI ACNGT 1 cut(s) 242
Bst6I CTCTTC 3 cut(s) 143, 365, 378
BstACI GRCGYC 1 cut(s) 77
BstC8I GCNNGC 1 cut(s) 132
BstDEI CTNAG 3 cut(s) 211, 231, 246
BstENI CCTNNNNNAGG 1 cut(s) 260
BstF5I GGATG 1 cut(s) 21
BstH2I RGCGCY 3 cut(s) 59, 80, 137
BstHHI GCGC 3 cut(s) 58, 79, 136
BstKTI GATC 1 cut(s) 114
BstMAI GTCTC 1 cut(s) 231
BstMBI GATC 1 cut(s) 111
BstMWI GCNNNNNNNGC 3 cut(s) 44, 47, 53
BstNSI RCATGY 1 cut(s) 300
BstSCI CCNGG 1 cut(s) 72
BstSFI CTRYAG 1 cut(s) 291
BstSLI GKGCMC 1 cut(s) 209
BstV1I GCAGC 2 cut(s) 214, 305
BstV2I GAAGAC 1 cut(s) 293
BsuRI GGCC 1 cut(s) 266
BtrI CACGTC 1 cut(s) 204
BtsCI GGATG 1 cut(s) 21
BtsI GCAGTG 2 cut(s) 17, 222
BtsIMutI CAGTG 2 cut(s) 17, 222
Cac8I GCNNGC 1 cut(s) 132
CfoI GCGC 3 cut(s) 58, 79, 136
Cfr10I RCCGGY 1 cut(s) 130
Cfr13I GGNCC 1 cut(s) 265
Csp6I GTAC 1 cut(s) 334
CviAII CATG 1 cut(s) 297
CviJI RGCY 5 cut(s) 85, 130, 166, 266, 354
CviKI_1 RGCY 5 cut(s) 85, 130, 166, 266, 354
CviQI GTAC 1 cut(s) 334
DdeI CTNAG 3 cut(s) 211, 231, 246
DinI GGCGCC 1 cut(s) 78
DpnI GATC 1 cut(s) 113
DpnII GATC 1 cut(s) 111
Eam1104I CTCTTC 3 cut(s) 143, 365, 378
EarI CTCTTC 3 cut(s) 143, 365, 378
Eco31I GGTCTC 1 cut(s) 231
Eco47III AGCGCT 1 cut(s) 57
EcoNI CCTNNNNNAGG 1 cut(s) 260
EgeI GGCGCC 1 cut(s) 78
EheI GGCGCC 1 cut(s) 78
FaeI CATG 1 cut(s) 300
FaiI YATR 2 cut(s) 30, 298
FalI AAGNNNNNCTT 2 cut(s) 83, 115
FaqI GGGAC 1 cut(s) 131
FatI CATG 1 cut(s) 296
Fnu4HI GCNGC 4 cut(s) 39, 48, 228, 294
FokI GGATG 1 cut(s) 28
Fsp4HI GCNGC 4 cut(s) 39, 48, 228, 294
GlaI GCGC 3 cut(s) 57, 78, 135
GluI GCNGC 4 cut(s) 39, 48, 228, 294
GsuI CTGGAG 1 cut(s) 110
HaeII RGCGCY 3 cut(s) 59, 80, 137
HaeIII GGCC 1 cut(s) 266
HapII CCGG 3 cut(s) 74, 131, 156
HhaI GCGC 3 cut(s) 58, 79, 136
Hin1I GRCGYC 1 cut(s) 77
Hin1II CATG 1 cut(s) 300
Hin6I GCGC 3 cut(s) 56, 77, 134
HinP1I GCGC 3 cut(s) 56, 77, 134
HindIII AAGCTT 1 cut(s) 352
HinfI GANTC 2 cut(s) 67, 159
HpaII CCGG 3 cut(s) 74, 131, 156
Hpy166II GTNNAC 1 cut(s) 207
Hpy188I TCNGA 2 cut(s) 214, 247
Hpy188III TCNNGA 1 cut(s) 64
Hpy8I GTNNAC 1 cut(s) 207
Hpy99I CGWCG 1 cut(s) 146
HpyAV CCTTC 1 cut(s) 160
HpyCH4III ACNGT 1 cut(s) 242
HpyCH4IV ACGT 2 cut(s) 26, 203
HpyCH4V TGCA 4 cut(s) 10, 207, 222, 293
HpyF10VI GCNNNNNNNGC 3 cut(s) 44, 47, 53
HpyF3I CTNAG 3 cut(s) 211, 231, 246
HpySE526I ACGT 2 cut(s) 26, 203
Hsp92I GRCGYC 1 cut(s) 77
Hsp92II CATG 1 cut(s) 300
HspAI GCGC 3 cut(s) 56, 77, 134
KasI GGCGCC 1 cut(s) 76
KroI GCCGGC 1 cut(s) 130
KroNI GCCGGC 1 cut(s) 132
Kzo9I GATC 1 cut(s) 111
LguI GCTCTTC 1 cut(s) 143
LmnI GCTCC 2 cut(s) 171, 190
LpnPI CCDG 8 cut(s) 77, 87, 140, 144, 166, 169, 176, 295
Lsp1109I GCAGC 2 cut(s) 214, 305
LweI GCATC 1 cut(s) 202
MaeII ACGT 2 cut(s) 26, 203
MalI GATC 1 cut(s) 113
MboI GATC 1 cut(s) 111
MboII GAAGA 4 cut(s) 130, 196, 293, 382
MhlI GDGCHC 1 cut(s) 209
MluCI AATT 1 cut(s) 279
Mly113I GGCGCC 1 cut(s) 77
MlyI GAGTC 1 cut(s) 61
MnlI CCTC 6 cut(s) 37, 179, 220, 266, 352, 366
MroNI GCCGGC 1 cut(s) 130
MroXI GAANNNNTTC 1 cut(s) 374
MspI CCGG 3 cut(s) 74, 131, 156
MspR9I CCNGG 1 cut(s) 74
MwoI GCNNNNNNNGC 3 cut(s) 44, 47, 53
NaeI GCCGGC 1 cut(s) 132
NarI GGCGCC 1 cut(s) 77
NciI CCSGG 1 cut(s) 74
NdeII GATC 1 cut(s) 111
NgoMIV GCCGGC 1 cut(s) 130
NlaIII CATG 1 cut(s) 300
NlaIV GGNNCC 1 cut(s) 78
NspI RCATGY 1 cut(s) 300
PciSI GCTCTTC 1 cut(s) 143
PdiI GCCGGC 1 cut(s) 132
PdmI GAANNNNTTC 1 cut(s) 374
PfeI GAWTC 1 cut(s) 159
PkrI GCNGC 4 cut(s) 40, 49, 229, 295
PleI GAGTC 1 cut(s) 61
PluTI GGCGCC 1 cut(s) 80
PpsI GAGTC 1 cut(s) 61
PspN4I GGNNCC 1 cut(s) 78
PspPI GGNCC 1 cut(s) 265
PstI CTGCAG 1 cut(s) 295
RsaI GTAC 1 cut(s) 335
RsaNI GTAC 1 cut(s) 334
SapI GCTCTTC 1 cut(s) 143
SatI GCNGC 4 cut(s) 39, 48, 228, 294
Sau3AI GATC 1 cut(s) 111
Sau96I GGNCC 1 cut(s) 265
ScaI AGTACT 1 cut(s) 335
SchI GAGTC 1 cut(s) 61
ScrFI CCNGG 1 cut(s) 74
SduI GDGCHC 1 cut(s) 209
SetI ASST 7 cut(s) 29, 87, 168, 206, 212, 356, 377
SfaNI GCATC 1 cut(s) 202
SfcI CTRYAG 1 cut(s) 291
SfoI GGCGCC 1 cut(s) 78
Sse9I AATT 1 cut(s) 279
SsiI CCGC 2 cut(s) 38, 47
SspDI GGCGCC 1 cut(s) 76
StyD4I CCNGG 1 cut(s) 72
TaaI ACNGT 1 cut(s) 242
TaiI ACGT 2 cut(s) 29, 206
TaqI TCGA 1 cut(s) 171
TasI AATT 1 cut(s) 279
TatI WGTACW 1 cut(s) 333
TauI GCSGC 2 cut(s) 41, 50
TfiI GAWTC 1 cut(s) 159
TscAI CASTG 2 cut(s) 17, 229
TseI GCWGC 2 cut(s) 227, 293
TspRI CASTG 2 cut(s) 17, 229
VneI GTGCAC 1 cut(s) 205
XagI CCTNNNNNAGG 1 cut(s) 260
XceI RCATGY 1 cut(s) 300
XmnI GAANNNNTTC 1 cut(s) 374
ZrmI AGTACT 1 cut(s) 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.