MD06G1232700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0011123
Physical Location & Seq
Forward (+)
36412627 .. 36414828
2202 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1232700.v1.1.491

Sequence Viewer

Length: 390 bp
ATGGCGTTGCAGTGGATGATACTGACGTACGTAGTGGCGGCAGAGGCGGCGTTGGCCTTCCTGCTAACCATTCCGGCGCCCAAGCTGTTGAAGAATCGATTCGTGTCGCTGGTCTCTCTAATCCTCCAACCCGCCCTCTTCATCGTCCCCTTCGCCGGATTTCAGCTCCTCGATATATATTGGAAGAATGAGCACCGCTTGGCCTGCACCTCTGATACTTGCACCGCCTCTGAGCGTGACCGCTATGAGAAATCCCTGTACAAGGCTCAAAGGAATGTAATTCTTTGTGCTTCAGCATGTCTGCTTTACTGGTGTCTATTTCGCATCTGTAAATATTACAAGGACATTCAGAACTTGGAGGAAGTGGAGAAGAGGTACAAGGACGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bap31

Protein Analysis

130

Amino Acids

15.07

Weight (kDa)

8.52

Isoelectric Point (pI)

46.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bap31 PF05529 1 - 121 3.9e-06 Bap31/Bap29 transmembrane region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 76
AciI CCGC 6 cut(s) 38, 47, 132, 196, 225, 241
AcuI CTGAAG 1 cut(s) 276
AcyI GRCGYC 1 cut(s) 77
AfaI GTAC 3 cut(s) 29, 260, 377
AfiI CCNNNNNNNGG 2 cut(s) 155, 262
AgsI TTSAA 1 cut(s) 91
AluBI AGCT 2 cut(s) 85, 166
AluI AGCT 2 cut(s) 85, 166
Alw21I GWGCWC 1 cut(s) 195
Alw26I GTCTC 1 cut(s) 118
AoxI GGCC 2 cut(s) 54, 201
Asp700I GAANNNNTTC 1 cut(s) 98
AspLEI GCGC 1 cut(s) 79
BaeI ACNNNNGTAYC 2 cut(s) 11, 44
BanI GGYRCC 1 cut(s) 76
Bbv12I GWGCWC 1 cut(s) 195
BcoDI GTCTC 1 cut(s) 118
BfoI RGCGCY 1 cut(s) 80
BisI GCNGC 2 cut(s) 39, 48
BlsI GCNGC 2 cut(s) 40, 49
BmiI GGNNCC 1 cut(s) 78
BmsI GCATC 1 cut(s) 333
Bsa29I ATCGAT 1 cut(s) 97
BsaAI YACGTR 1 cut(s) 31
BsaHI GRCGYC 1 cut(s) 77
BsaI GGTCTC 1 cut(s) 118
BsaXI ACNNNNNCTCC 2 cut(s) 359, 389
Bsc4I CCNNNNNNNGG 2 cut(s) 155, 262
Bse1I ACTGG 1 cut(s) 314
BseCI ATCGAT 1 cut(s) 97
BseGI GGATG 1 cut(s) 21
BseLI CCNNNNNNNGG 2 cut(s) 155, 262
BseMII CTCAG 1 cut(s) 222
BseNI ACTGG 1 cut(s) 314
BseRI GAGGAG 1 cut(s) 158
BsgI GTGCAG 1 cut(s) 190
BshFI GGCC 2 cut(s) 56, 203
BshNI GGYRCC 1 cut(s) 76
BshVI ATCGAT 1 cut(s) 97
BsiHKAI GWGCWC 1 cut(s) 195
BsiSI CCGG 2 cut(s) 74, 156
BsiWI CGTACG 1 cut(s) 27
BslFI GGGAC 1 cut(s) 131
BslI CCNNNNNNNGG 2 cut(s) 155, 262
BsmAI GTCTC 1 cut(s) 118
BsmFI GGGAC 1 cut(s) 131
BsnI GGCC 2 cut(s) 56, 203
Bso31I GGTCTC 1 cut(s) 118
Bsp1286I GDGCHC 1 cut(s) 195
Bsp1407I TGTACA 1 cut(s) 258
BspACI CCGC 6 cut(s) 38, 47, 132, 196, 225, 241
BspANI GGCC 2 cut(s) 56, 203
BspCNI CTCAG 1 cut(s) 223
BspDI ATCGAT 1 cut(s) 97
BspLI GGNNCC 1 cut(s) 78
BspT107I GGYRCC 1 cut(s) 76
BspTNI GGTCTC 1 cut(s) 118
BsrGI TGTACA 1 cut(s) 258
BsrI ACTGG 1 cut(s) 314
BssNI GRCGYC 1 cut(s) 77
Bst6I CTCTTC 2 cut(s) 143, 365
BstACI GRCGYC 1 cut(s) 77
BstAUI TGTACA 1 cut(s) 258
BstBAI YACGTR 1 cut(s) 31
BstC8I GCNNGC 1 cut(s) 205
BstDEI CTNAG 1 cut(s) 231
BstENI CCTNNNNNAGG 1 cut(s) 260
BstF5I GGATG 1 cut(s) 21
BstH2I RGCGCY 1 cut(s) 80
BstHHI GCGC 1 cut(s) 79
BstMAI GTCTC 1 cut(s) 118
BstMWI GCNNNNNNNGC 4 cut(s) 44, 47, 53, 204
BstNSI RCATGY 1 cut(s) 300
BstSNI TACGTA 1 cut(s) 31
Bsu15I ATCGAT 1 cut(s) 97
BsuRI GGCC 2 cut(s) 56, 203
BsuTUI ATCGAT 1 cut(s) 97
BtsCI GGATG 1 cut(s) 21
BtsI GCAGTG 1 cut(s) 17
BtsIMutI CAGTG 1 cut(s) 17
Cac8I GCNNGC 1 cut(s) 205
CfoI GCGC 1 cut(s) 79
ClaI ATCGAT 1 cut(s) 97
Csp6I GTAC 3 cut(s) 28, 259, 376
CviAII CATG 1 cut(s) 297
CviJI RGCY 5 cut(s) 56, 85, 166, 203, 266
CviKI_1 RGCY 5 cut(s) 56, 85, 166, 203, 266
CviQI GTAC 3 cut(s) 28, 259, 376
DdeI CTNAG 1 cut(s) 231
DinI GGCGCC 1 cut(s) 78
Eam1104I CTCTTC 2 cut(s) 143, 365
EarI CTCTTC 2 cut(s) 143, 365
Eco105I TACGTA 1 cut(s) 31
Eco31I GGTCTC 1 cut(s) 118
Eco57I CTGAAG 1 cut(s) 276
EcoNI CCTNNNNNAGG 1 cut(s) 260
EgeI GGCGCC 1 cut(s) 78
EheI GGCGCC 1 cut(s) 78
FaeI CATG 1 cut(s) 300
FaiI YATR 4 cut(s) 176, 178, 246, 298
FaqI GGGAC 1 cut(s) 131
FatI CATG 1 cut(s) 296
FauI CCCGC 1 cut(s) 139
Fnu4HI GCNGC 2 cut(s) 39, 48
FokI GGATG 1 cut(s) 28
Fsp4HI GCNGC 2 cut(s) 39, 48
GlaI GCGC 1 cut(s) 78
GluI GCNGC 2 cut(s) 39, 48
HaeII RGCGCY 1 cut(s) 80
HaeIII GGCC 2 cut(s) 56, 203
HapII CCGG 2 cut(s) 74, 156
HhaI GCGC 1 cut(s) 79
Hin1I GRCGYC 1 cut(s) 77
Hin1II CATG 1 cut(s) 300
Hin6I GCGC 1 cut(s) 77
HinP1I GCGC 1 cut(s) 77
HinfI GANTC 2 cut(s) 94, 99
HpaII CCGG 2 cut(s) 74, 156
Hpy188I TCNGA 3 cut(s) 214, 232, 351
HpyAV CCTTC 2 cut(s) 67, 160
HpyCH4IV ACGT 2 cut(s) 26, 30
HpyCH4V TGCA 3 cut(s) 10, 207, 222
HpyF10VI GCNNNNNNNGC 4 cut(s) 44, 47, 53, 204
HpyF3I CTNAG 1 cut(s) 231
HpySE526I ACGT 2 cut(s) 26, 30
Hsp92I GRCGYC 1 cut(s) 77
Hsp92II CATG 1 cut(s) 300
HspAI GCGC 1 cut(s) 77
KasI GGCGCC 1 cut(s) 76
LmnI GCTCC 1 cut(s) 171
LpnPI CCDG 7 cut(s) 74, 87, 95, 169, 217, 269, 295
LweI GCATC 1 cut(s) 333
MaeII ACGT 2 cut(s) 26, 30
MaeIII GTNAC 1 cut(s) 236
MboII GAAGA 4 cut(s) 103, 130, 196, 382
MhlI GDGCHC 1 cut(s) 195
MluCI AATT 1 cut(s) 279
Mly113I GGCGCC 1 cut(s) 77
MmeI TCCRAC 1 cut(s) 151
MnlI CCTC 8 cut(s) 37, 134, 146, 179, 220, 238, 352, 366
MroXI GAANNNNTTC 1 cut(s) 98
MspI CCGG 2 cut(s) 74, 156
MwoI GCNNNNNNNGC 4 cut(s) 44, 47, 53, 204
NarI GGCGCC 1 cut(s) 77
NlaIII CATG 1 cut(s) 300
NlaIV GGNNCC 1 cut(s) 78
NmuCI GTSAC 1 cut(s) 236
NspI RCATGY 1 cut(s) 300
PdmI GAANNNNTTC 1 cut(s) 98
PfeI GAWTC 2 cut(s) 94, 99
Pfl23II CGTACG 1 cut(s) 27
PkrI GCNGC 2 cut(s) 40, 49
PluTI GGCGCC 1 cut(s) 80
Ppu21I YACGTR 1 cut(s) 31
PspLI CGTACG 1 cut(s) 27
PspN4I GGNNCC 1 cut(s) 78
RsaI GTAC 3 cut(s) 29, 260, 377
RsaNI GTAC 3 cut(s) 28, 259, 376
SatI GCNGC 2 cut(s) 39, 48
SduI GDGCHC 1 cut(s) 195
SetI ASST 6 cut(s) 29, 33, 87, 168, 212, 377
SfaNI GCATC 1 cut(s) 333
SfoI GGCGCC 1 cut(s) 78
SnaBI TACGTA 1 cut(s) 31
Sse9I AATT 1 cut(s) 279
SsiI CCGC 6 cut(s) 38, 47, 132, 196, 225, 241
SspDI GGCGCC 1 cut(s) 76
SspI AATATT 1 cut(s) 335
TaiI ACGT 2 cut(s) 29, 33
TaqI TCGA 2 cut(s) 97, 171
TasI AATT 1 cut(s) 279
TatI WGTACW 1 cut(s) 258
TauI GCSGC 2 cut(s) 41, 50
TfiI GAWTC 2 cut(s) 94, 99
TscAI CASTG 1 cut(s) 17
TseFI GTSAC 1 cut(s) 236
Tsp45I GTSAC 1 cut(s) 236
TspDTI ATGAA 1 cut(s) 130
TspRI CASTG 1 cut(s) 17
XagI CCTNNNNNAGG 1 cut(s) 260
XceI RCATGY 1 cut(s) 300
XmnI GAANNNNTTC 1 cut(s) 98
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.