Rorug06G0411200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0011123
Physical Location & Seq
Forward (+)
55333857 .. 55342588
8732 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0411200.1

Sequence Viewer

Length: 3738 bp
ATGGAGCCGCAGGACGACGAGTCCATGGCCGATGGCTCCGACTCGGCTCGTGGACCGTCCAGGGGCCCAAGGCTGTTCATCAGAGAAATGGTCATGAGGAACTTCAAGTCTTACGCCGGCGAGCAACGGGTCGGTCCTTTCCACAAGAGCTTTTCTGCAGTTGTTGGACCAAATGGGAGTGGTAAAAGCAATGTGATAGATGCAATGCTCTTTGTATTTGGAAAACGAGCTAAGCAGATGCGACTGAACAAAGTTTCGGAGCTTATACATAACTCCACGAATCACCAGAATCTGGATAGTGCTGGAGTTTCTGTTCATTTCCAAGAGATCATTGATATGGATGATGGAGAATTTGAAGTTGTTCCAGGAAGTGATTTTGTAATTACGAGAATAGCATATCGGGATAACACCTCCAAGTATTACATCAATGACCGTGCAAGTAATTTCACTGAAGTTACCAAGAAACTGAAAGGGAAAGGAGTTGACCTAGACAACAATCGTTTTTTGATTCTTCAGGGTGAAGTTGAGCAGATTTCACTTATGAAGCCAAAAGCTCAAGGATCCCATGATGAAGGTTTTCTTGAATACCTCGAGGACATTATTGGGACTAACAAATATGTTGAAAAAATTGATGAAGCATATAAAGAGCTAGAATCCCTTAATGAGAAGAGGTCTGGTGTTGTGCAAATGGTTAAACTGGCTGAGAAAGAAAGAGATGCCTTGGAGGATGTGAAAAATGAAGCGGAAGCCTATATGCTGAAAGAGTTGTCACTTCTGAAATGGCAAGAGAAAGCCACAAAATTGGCTCATGAAGATACCACTGCAAAAATGGCTGATCTAAAGGAGAATATAACTAGCTTAGAAGAAAATCTAAAGACTGAAAGGGAGAAGATCCAGGAGAGTAATAATGCACTGAAGGAGCTTGATTCTGTCTACAGTAAACATATGAAACAACAAGAGGAACTTGATAATGCCCTCAGAAGCAGTAATGAAGAATTTAAGGAGTTTGAAAGGGAGGATCTTAAGTATAGGGAAGATCTGAAGCATAAGAAGATGAAGATCAAGAAACTCAAAGATAAACTTCAAAAGGACTCGTTAAAGATTGCTGACACAGAGAAGGAGTGTGAGGACTCGACAAATCTGATCCCAAAACTTGAGGAAAGTATTCCGCAACTGCAAAAACGATTGTTGGATGAAGAAAAAGTCCTAGAGGAAATTCTAGAGAATTCAAAAGTGGAAACTGAAAAATATCGCTCTGAGCTTACAAAAGTTCGTGCTGAATTAGAACCATGGGAAAAGCAACTGATTGAGCACAAGGGGAAACTCGAAGTTAAATGTACTGAACAAAAGCTTTTAAATGAGAAGCATGAAGCTGGTCGTGCATCTTTTGAAGATGCTCGTAAGCAGATGGATGATATATTGGGAAGGATAGAAACAAAAACTGCGGGCATTGCAAGGATTCAAAGAGAATTAGAAATGAGCAAGCGCGAAGCAATGGAATTTCGCAAAGAGGAACAAGAATACATCAGAGAACAAGAGGCTCTCATTCCTAGTGAACAGGCTGCAAGACAGAAGGTTGCAGAACTGAAGTCTGTTCTGGATTCTGAGAAGAGTCAGGGAACAGTTCTAAAAGCAATTCTACATGCTAAGGATTCCAACCGGATACCGGGAATATATGGACGGATGGGCGATTTAGGTGCTATTGATGCAAAATATGATGTTGCCATATCAACAGCTTGTGCCGGACTTGATTATATTGTGGTGGAAACAACTATTGCAGCACAATCATGTGTTGAGCTACTCCGGAGAGAGAACCTTGGAATTGTGACTTGCATGATATTGGAAAAGCAAGCTGATCTCTTACCAAAGTTGAAGGAGAAGATAAGCACTCCAGAAGGAGTTCCTCGCCTTTTTGATTTAGTTAGGGTGCAGGATGAGAGAATGAAACTTGCTTTTTATGCAGCATTGCGAAACACTGTTGTAGCTAAAGATCTTGACCAGGCAACACGAATTGCATATGGAGGGAATCACTATTTTCGGCGAGTGGTAACACTTGATGGTGCACTATTTGAAAATTCTGGCACTATGAGTGGCGGGGGAGGTAAGCCACGTGGTGGCAAGATGGGCACTTCTGTTCGTGCCACCAGTGTGTCAGGAGAAGCTGTTGCGATTGCTGAAAAGGAGCTTGAAGCCATGGCTTCTTCATTAAATAATTTGCGCCAGAAAATTGCTGATGCAGCAAGGAGATATCAAGCCTCTGAAAATGCAGTTGCACGCTTGGAGATGGACTTAGCTAAAAGCCAGAAAGAGATTGACAGCCTGAATTCGCAACATGATTATCTTGAGAAGCAAATTGGTTCCCTGGAGGCTGCATCACAGCCGAAGAAGGATGAGCTTGAGAAATTGGATGAACTCAAGAAGCATATTGCTGCAGAAGAAAAAGAGATTCAGAAACTTATACAAGGGTCTCAGCAGCTGAAGGAGAAGGCCACGAAACTTCAGAGCAACATAGAAAATGCTGGAGGTGAGAGGTTAAAAGCACAGAAGTCAAAAGTCAAGGACATTCAGTCTGATATTGACAAAAATAACACTGAGGTTAACCGTCGCAAAGTCCAAATTGAGACTGGCCAGAAAACTATAAAGAAGTTAACCAATGTGATTGAAGAATCAAAGAATGAGATAGATCGACTTGGTAAGGAGAAGGAAAATTTGTCTGGCAAGTTCAAAGAAATAGAAAAAAAGGCATTTGAAGTTAAAGAGAAATACGAGCGAACACAGAAGCTGATTGATAATCATAAAGATGTGTTAGATAAAGCGAAATCTGACTATGACAATATGAAAAAGACTTTGTATGAATTGCGGGCATCAGAGGGTAATGCTGTTTTTCAATTACAAGAAAATAAGAAGGTATACAAGGAGCAGGAATTGAAGGGAAGGCATTACAATAAAAAGCTTGATGACTTGCAAACTGATCTTATGAAGCATATGGAACAAATTCAGAAAGATTTAGTGGACGCGGAAAAGCTTCAAGCAACCCTAGCAGATGAAATTCTTAATAACCCTTGTGACCTTAAAAAGGGCCTTGAAATGGTAGCGCTTCTGGAAGCACAATTGAAAGAGATGAATCCGAATCTTGATTCAGTCGCAGAGTATCGGAGAAAGGTCTCATTATATAATGAACGAGTTGAGGATCTTAATAGGGTCACTCAACAGCGTGATGACATAAAGAGGCAGTATGATGAATGGAGAAAGAAGAGGTTGGATGAGTTCATGGCAGGATTTAATGCTATATCTTTGAAGTTAAAGGAAATGTATCAGATGATTACACTGGGAGGTGATGCAGAACTTGAGCTAGTGGACTCTCTGGATCCCTTCTCTGAAGGTGTTGTTTTCAGTGTGAGACCACCAAAGAAGAGTTGGAAGAATATTGCAAACTTGTCTGGTGGTGAAAAGACGTTGAGCTCATTAGCTCTTGTTTTTGCACTGCATCATTACAAGCCTACACCTCTTTATGTAATGGATGAAATCGATGCCGCCCTAGACTTCAAGAATGTCTCTATTGTGGGTCATTATGTAAAGGACAGGACCAAGGATGCACAGTTCATAATCATAAGCCTTAGAAACAATATGTTCGAGCTGGCGGATCGACTTGTTGGGATATACAAAACTGATAATTGCACAAAAAGCATTACCATTGATCCAGGGAGGTTCGTGGTTTGTGAAAATGCAGCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bap31

Protein Analysis

1245

Amino Acids

141.91

Weight (kDa)

5.97

Isoelectric Point (pI)

38.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SMC_N PF02463 26 - 1227 5.4e-59 RecF/RecN/SMC N terminal domain
AAA_15 PF13175 27 - 410 2.2e-06 AAA ATPase domain
AAA_29 PF13555 29 - 86 7.9e-06 P-loop containing region of AAA domain
AAA_23 PF13476 30 - 292 4.3e-10 AAA domain
AAA_21 PF13304 51 - 120 4.8e-06 AAA domain, putative AbiEii toxin, Type IV TA system
SMC_hinge PF06470 556 - 672 2.8e-20 SMC proteins Flexible Hinge Domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 590
AccB7I CCANNNNNTGG 2 cut(s) 292, 2114
AccI GTMKAC 2 cut(s) 933, 2910
AccII CGCG 2 cut(s) 1488, 3017
AccIII TCCGGA 1 cut(s) 1803
AciI CCGC 9 cut(s) 8, 743, 1169, 1445, 2094, 2860, 3017, 3534, 3641
AcoI YGGCCR 2 cut(s) 27, 2626
AcuI CTGAAG 8 cut(s) 471, 497, 935, 1061, 1607, 2483, 2498, 3399
AcvI CACGTG 1 cut(s) 2111
AdeI CACNNNGTG 1 cut(s) 2114
AfaI GTAC 1 cut(s) 1339
AfeI AGCGCT 1 cut(s) 3096
AfiI CCNNNNNNNGG 6 cut(s) 62, 292, 1666, 2114, 3076, 3088
AflII CTTAAG 1 cut(s) 1022
AhdI GACNNNNNGTC 2 cut(s) 19, 2566
AjnI CCWGG 6 cut(s) 59, 364, 894, 1998, 2361, 3700
AleI CACNNNNGTG 1 cut(s) 2147
AloI GAACNNNNNNTCC 4 cut(s) 297, 329, 3584, 3616
Alw21I GWGCWC 3 cut(s) 1314, 2065, 3464
Alw26I GTCTC 5 cut(s) 2472, 2615, 3169, 3394, 3559
Alw44I GTGCAC 1 cut(s) 2061
AlwNI CAGNNNCTG 3 cut(s) 292, 2476, 2782
Ama87I CYCGRG 1 cut(s) 590
Aor13HI TCCGGA 1 cut(s) 1803
Aor51HI AGCGCT 1 cut(s) 3096
AoxI GGCC 5 cut(s) 27, 64, 2487, 2626, 3079
ApaI GGGCCC 1 cut(s) 68
ApaLI GTGCAC 1 cut(s) 2061
ApeKI GCWGC 9 cut(s) 1562, 1778, 1961, 2237, 2369, 2429, 2473, 3728, 3731
Asp700I GAANNNNTTC 6 cut(s) 360, 576, 1164, 1899, 2994, 3024
AspLEI GCGC 3 cut(s) 1488, 2220, 3097
AspS9I GGNCC 7 cut(s) 53, 64, 65, 134, 167, 3079, 3585
AsuC2I CCSGG 1 cut(s) 1668
AsuHPI GGTGA 5 cut(s) 275, 530, 2537, 3347, 3458
AvaI CYCGRG 1 cut(s) 590
AvaII GGWCC 4 cut(s) 53, 134, 167, 3585
BaeGI GKGCMC 3 cut(s) 68, 2065, 2129
BalI TGGCCA 1 cut(s) 2628
BamHI GGATCC 2 cut(s) 560, 3367
BanII GRGCYC 2 cut(s) 68, 3464
BauI CACGAG 1 cut(s) 48
BbrPI CACGTG 1 cut(s) 2111
Bbv12I GWGCWC 3 cut(s) 1314, 2065, 3464
BbvI GCAGC 8 cut(s) 1549, 1790, 1973, 2249, 2356, 2416, 2485, 3718
BccI CCATC 7 cut(s) 26, 338, 1402, 1678, 2051, 2116, 2278
BcgI CGANNNNNNTGC 2 cut(s) 1679, 1713
BciT130I CCWGG 6 cut(s) 61, 366, 896, 2000, 2363, 3702
BciVI GTATCC 1 cut(s) 1656
BcnI CCSGG 1 cut(s) 1668
BcoDI GTCTC 5 cut(s) 2472, 2615, 3169, 3394, 3559
BfaI CTAG 9 cut(s) 488, 650, 855, 1208, 1220, 1551, 3038, 3353, 3539
BfmI CTRYAG 3 cut(s) 156, 934, 2430
BfoI RGCGCY 1 cut(s) 3098
BfrI CTTAAG 1 cut(s) 1022
BfuI GTATCC 1 cut(s) 1656
BglII AGATCT 2 cut(s) 1036, 1990
BlpI GCTNAGC 1 cut(s) 231
Bme1390I CCNGG 7 cut(s) 61, 366, 896, 1668, 2000, 2363, 3702
Bme18I GGWCC 4 cut(s) 53, 134, 167, 3585
BmeRI GACNNNNNGTC 2 cut(s) 19, 2566
BmeT110I CYCGRG 1 cut(s) 590
BmgT120I GGNCC 7 cut(s) 53, 64, 65, 134, 167, 3079, 3585
BmiI GGNNCC 7 cut(s) 6, 37, 65, 66, 562, 2359, 3369
BmrFI CCNGG 7 cut(s) 61, 366, 896, 1668, 2000, 2363, 3702
BmrI ACTGGG 1 cut(s) 3338
BmuI ACTGGG 1 cut(s) 3338
BpmI CTGGAG 4 cut(s) 324, 1875, 2384, 2541
Bpu10I CCTNAGC 1 cut(s) 1647
Bpu1102I GCTNAGC 1 cut(s) 231
BpuEI CTTGAG 6 cut(s) 540, 1175, 2363, 2399, 2417, 3368
BpuMI CCSGG 1 cut(s) 1668
Bsa29I ATCGAT 1 cut(s) 3528
BsaAI YACGTR 1 cut(s) 2111
BsaBI GATNNNNATC 1 cut(s) 1058
BsaI GGTCTC 3 cut(s) 2472, 3169, 3394
BsaWI WCCGGW 2 cut(s) 1659, 1803
BsaXI ACNNNNNCTCC 4 cut(s) 297, 327, 2148, 2178
Bsc4I CCNNNNNNNGG 6 cut(s) 62, 292, 1666, 2114, 3076, 3088
Bse118I RCCGGY 1 cut(s) 116
Bse1I ACTGG 4 cut(s) 702, 2145, 2629, 3333
Bse3DI GCAATG 5 cut(s) 196, 210, 1449, 1500, 1964
Bse8I GATNNNNATC 1 cut(s) 1058
BseAI TCCGGA 1 cut(s) 1803
BseBI CCWGG 6 cut(s) 61, 366, 896, 2000, 2363, 3702
BseCI ATCGAT 1 cut(s) 3528
BseJI GATNNNNATC 1 cut(s) 1058
BseLI CCNNNNNNNGG 6 cut(s) 62, 292, 1666, 2114, 3076, 3088
BseMI GCAATG 5 cut(s) 196, 210, 1449, 1500, 1964
BseMII CTCAG 6 cut(s) 693, 991, 1248, 1596, 2483, 2583
BseNI ACTGG 4 cut(s) 702, 2145, 2629, 3333
BseSI GKGCMC 3 cut(s) 68, 2065, 2129
BseXI GCAGC 8 cut(s) 1549, 1790, 1973, 2249, 2356, 2416, 2485, 3718
BsgI GTGCAG 1 cut(s) 1949
Bsh1236I CGCG 2 cut(s) 1488, 3017
BshFI GGCC 5 cut(s) 29, 66, 2489, 2628, 3081
BshVI ATCGAT 1 cut(s) 3528
BsiHKAI GWGCWC 3 cut(s) 1314, 2065, 3464
BsiHKCI CYCGRG 1 cut(s) 590
BsiSI CCGG 5 cut(s) 117, 1660, 1667, 1743, 1804
BslFI GGGAC 1 cut(s) 619
BslI CCNNNNNNNGG 6 cut(s) 62, 292, 1666, 2114, 3076, 3088
BsmAI GTCTC 5 cut(s) 2472, 2615, 3169, 3394, 3559
BsmFI GGGAC 1 cut(s) 619
BsnI GGCC 5 cut(s) 29, 66, 2489, 2628, 3081
Bso31I GGTCTC 3 cut(s) 2472, 3169, 3394
BsoBI CYCGRG 1 cut(s) 590
Bsp120I GGGCCC 1 cut(s) 64
Bsp1286I GDGCHC 5 cut(s) 68, 1314, 2065, 2129, 3464
Bsp13I TCCGGA 1 cut(s) 1803
Bsp1720I GCTNAGC 1 cut(s) 231
Bsp19I CCATGG 3 cut(s) 24, 1289, 2193
BspACI CCGC 9 cut(s) 8, 743, 1169, 1445, 2094, 2860, 3017, 3534, 3641
BspANI GGCC 5 cut(s) 29, 66, 2489, 2628, 3081
BspCNI CTCAG 6 cut(s) 694, 990, 1249, 1597, 2482, 2584
BspDI ATCGAT 1 cut(s) 3528
BspEI TCCGGA 1 cut(s) 1803
BspFNI CGCG 2 cut(s) 1488, 3017
BspHI TCATGA 2 cut(s) 93, 808
BspLI GGNNCC 7 cut(s) 6, 37, 65, 66, 562, 2359, 3369
BspMAI CTGCAG 2 cut(s) 160, 2434
BspTI CTTAAG 1 cut(s) 1022
BspTNI GGTCTC 3 cut(s) 2472, 3169, 3394
BsrDI GCAATG 5 cut(s) 196, 210, 1449, 1500, 1964
BsrFI RCCGGY 1 cut(s) 116
BsrI ACTGG 4 cut(s) 702, 2145, 2629, 3333
BssAI RCCGGY 1 cut(s) 116
BssNAI GTATAC 1 cut(s) 2911
BssSI CACGAG 1 cut(s) 48
BssT1I CCWWGG 7 cut(s) 24, 68, 720, 1289, 1816, 2193, 3588
Bst1107I GTATAC 1 cut(s) 2911
Bst2BI CACGAG 1 cut(s) 48
Bst2UI CCWGG 6 cut(s) 61, 366, 896, 2000, 2363, 3702
Bst4CI ACNGT 7 cut(s) 57, 434, 938, 1624, 1978, 2603, 3600
Bst6I CTCTTC 4 cut(s) 662, 1604, 3248, 3407
BstAFI CTTAAG 1 cut(s) 1022
BstBAI YACGTR 1 cut(s) 2111
BstC8I GCNNGC 8 cut(s) 118, 122, 1447, 1484, 1851, 2275, 2862, 3639
BstDSI CCRYGG 3 cut(s) 24, 1289, 2193
BstENI CCTNNNNNAGG 1 cut(s) 3074
BstFNI CGCG 2 cut(s) 1488, 3017
BstH2I RGCGCY 1 cut(s) 3098
BstHHI GCGC 3 cut(s) 1488, 2220, 3097
BstMAI GTCTC 5 cut(s) 2472, 2615, 3169, 3394, 3559
BstMWI GCNNNNNNNGC 9 cut(s) 830, 1379, 1451, 1706, 1958, 2124, 2237, 3038, 3684
BstNI CCWGG 6 cut(s) 61, 366, 896, 2000, 2363, 3702
BstNSI RCATGY 1 cut(s) 1646
BstSCI CCNGG 7 cut(s) 59, 364, 894, 1666, 1998, 2361, 3700
BstSFI CTRYAG 3 cut(s) 156, 934, 2430
BstSLI GKGCMC 3 cut(s) 68, 2065, 2129
BstUI CGCG 2 cut(s) 1488, 3017
BstV1I GCAGC 8 cut(s) 1549, 1790, 1973, 2249, 2356, 2416, 2485, 3718
BstX2I RGATCY 7 cut(s) 560, 891, 1018, 1036, 1990, 3190, 3367
BstXI CCANNNNNNTGG 1 cut(s) 802
BstYI RGATCY 7 cut(s) 560, 891, 1018, 1036, 1990, 3190, 3367
BstZ17I GTATAC 1 cut(s) 2911
Bsu15I ATCGAT 1 cut(s) 3528
BsuI GTATCC 1 cut(s) 1656
BsuRI GGCC 5 cut(s) 29, 66, 2489, 2628, 3081
BsuTUI ATCGAT 1 cut(s) 3528
BtgI CCRYGG 3 cut(s) 24, 1289, 2193
BtsI GCAGTG 2 cut(s) 819, 3482
BtsIMutI CAGTG 9 cut(s) 447, 819, 911, 1974, 2152, 2589, 3326, 3400, 3482
Cac8I GCNNGC 8 cut(s) 118, 122, 1447, 1484, 1851, 2275, 2862, 3639
CaiI CAGNNNCTG 3 cut(s) 292, 2476, 2782
CciI TCATGA 2 cut(s) 93, 808
CfoI GCGC 3 cut(s) 1488, 2220, 3097
Cfr10I RCCGGY 1 cut(s) 116
Cfr13I GGNCC 7 cut(s) 53, 64, 65, 134, 167, 3079, 3585
ClaI ATCGAT 1 cut(s) 3528
CseI GACGC 1 cut(s) 3023
Csp6I GTAC 1 cut(s) 1338
CviQI GTAC 1 cut(s) 1338
DraI TTTAAA 1 cut(s) 1356
DraIII CACNNNGTG 1 cut(s) 2114
DriI GACNNNNNGTC 2 cut(s) 19, 2566
EaeI YGGCCR 2 cut(s) 27, 2626
Eam1104I CTCTTC 4 cut(s) 662, 1604, 3248, 3407
Eam1105I GACNNNNNGTC 2 cut(s) 19, 2566
EarI CTCTTC 4 cut(s) 662, 1604, 3248, 3407
EciI GGCGGA 1 cut(s) 3656
Ecl136II GAGCTC 1 cut(s) 3462
Eco130I CCWWGG 7 cut(s) 24, 68, 720, 1289, 1816, 2193, 3588
Eco24I GRGCYC 2 cut(s) 68, 3464
Eco31I GGTCTC 3 cut(s) 2472, 3169, 3394
Eco32I GATATC 1 cut(s) 2249
Eco47I GGWCC 4 cut(s) 53, 134, 167, 3585
Eco47III AGCGCT 1 cut(s) 3096
Eco53kI GAGCTC 1 cut(s) 3462
Eco57I CTGAAG 8 cut(s) 471, 497, 935, 1061, 1607, 2483, 2498, 3399
Eco72I CACGTG 1 cut(s) 2111
Eco88I CYCGRG 1 cut(s) 590
EcoICRI GAGCTC 1 cut(s) 3462
EcoNI CCTNNNNNAGG 1 cut(s) 3074
EcoO109I RGGNCCY 2 cut(s) 64, 3079
EcoRI GAATTC 2 cut(s) 1225, 2323
EcoRII CCWGG 6 cut(s) 59, 364, 894, 1998, 2361, 3700
EcoRV GATATC 1 cut(s) 2249
EcoT14I CCWWGG 7 cut(s) 24, 68, 720, 1289, 1816, 2193, 3588
EcoT38I GRGCYC 2 cut(s) 68, 3464
ErhI CCWWGG 7 cut(s) 24, 68, 720, 1289, 1816, 2193, 3588
FalI AAGNNNNNCTT 6 cut(s) 564, 596, 948, 980, 1065, 1097
FaqI GGGAC 1 cut(s) 619
FauI CCCGC 3 cut(s) 1438, 2087, 2853
FauNDI CATATG 3 cut(s) 945, 2017, 2985
FblI GTMKAC 2 cut(s) 933, 2910
FriOI GRGCYC 2 cut(s) 68, 3464
FspBI CTAG 9 cut(s) 488, 650, 855, 1208, 1220, 1551, 3038, 3353, 3539
GlaI GCGC 3 cut(s) 1487, 2219, 3096
GsuI CTGGAG 4 cut(s) 324, 1875, 2384, 2541
HaeII RGCGCY 1 cut(s) 3098
HaeIII GGCC 5 cut(s) 29, 66, 2489, 2628, 3081
HapII CCGG 5 cut(s) 117, 1660, 1667, 1743, 1804
HgaI GACGC 1 cut(s) 3023
HhaI GCGC 3 cut(s) 1488, 2220, 3097
Hin6I GCGC 3 cut(s) 1486, 2218, 3095
HinP1I GCGC 3 cut(s) 1486, 2218, 3095
HincII GTYRAC 3 cut(s) 484, 2599, 2649
HindII GTYRAC 3 cut(s) 484, 2599, 2649
HindIII AAGCTT 3 cut(s) 1349, 2951, 3023
HpaI GTTAAC 2 cut(s) 2599, 2649
HpaII CCGG 5 cut(s) 117, 1660, 1667, 1743, 1804
HphI GGTGA 5 cut(s) 275, 530, 2537, 3347, 3458
Hpy99I CGWCG 2 cut(s) 20, 2607
HpyCH4III ACNGT 7 cut(s) 57, 434, 938, 1624, 1978, 2603, 3600
HpyCH4IV ACGT 2 cut(s) 2110, 3455
HpyF10VI GCNNNNNNNGC 9 cut(s) 830, 1379, 1451, 1706, 1958, 2124, 2237, 3038, 3684
HpySE526I ACGT 2 cut(s) 2110, 3455
HspAI GCGC 3 cut(s) 1486, 2218, 3095
Kpn2I TCCGGA 1 cut(s) 1803
KroI GCCGGC 1 cut(s) 116
KroNI GCCGGC 1 cut(s) 118
KspAI GTTAAC 2 cut(s) 2599, 2649
LmnI GCTCC 6 cut(s) 4, 41, 259, 919, 2182, 2917
Lsp1109I GCAGC 8 cut(s) 1549, 1790, 1973, 2249, 2356, 2416, 2485, 3718
MaeI CTAG 9 cut(s) 488, 650, 855, 1208, 1220, 1551, 3038, 3353, 3539
MaeII ACGT 2 cut(s) 2110, 3455
MaeIII GTNAC 6 cut(s) 454, 768, 1825, 2047, 3065, 3202
MfeI CAATTG 1 cut(s) 3110
MflI RGATCY 7 cut(s) 560, 891, 1018, 1036, 1990, 3190, 3367
MhlI GDGCHC 5 cut(s) 68, 1314, 2065, 2129, 3464
MlsI TGGCCA 1 cut(s) 2628
MluNI TGGCCA 1 cut(s) 2628
MlyI GAGTC 6 cut(s) 29, 35, 1085, 1124, 1621, 3353
MmeI TCCRAC 6 cut(s) 63, 145, 1170, 1680, 3240, 3398
Mox20I TGGCCA 1 cut(s) 2628
MreI CGCCGGCG 1 cut(s) 116
MroI TCCGGA 1 cut(s) 1803
MroNI GCCGGC 1 cut(s) 116
MroXI GAANNNNTTC 6 cut(s) 360, 576, 1164, 1899, 2994, 3024
MscI TGGCCA 1 cut(s) 2628
MslI CAYNNNNRTG 4 cut(s) 335, 1786, 2147, 2799
Msp20I TGGCCA 1 cut(s) 2628
MspA1I CMGCKG 2 cut(s) 2476, 3731
MspCI CTTAAG 1 cut(s) 1022
MspI CCGG 5 cut(s) 117, 1660, 1667, 1743, 1804
MspR9I CCNGG 7 cut(s) 61, 366, 896, 1668, 2000, 2363, 3702
MunI CAATTG 1 cut(s) 3110
MvaI CCWGG 6 cut(s) 61, 366, 896, 2000, 2363, 3702
MvnI CGCG 2 cut(s) 1488, 3017
MwoI GCNNNNNNNGC 9 cut(s) 830, 1379, 1451, 1706, 1958, 2124, 2237, 3038, 3684
NaeI GCCGGC 1 cut(s) 118
NciI CCSGG 1 cut(s) 1668
NcoI CCATGG 3 cut(s) 24, 1289, 2193
NdeI CATATG 3 cut(s) 945, 2017, 2985
NgoMIV GCCGGC 1 cut(s) 116
NlaIV GGNNCC 7 cut(s) 6, 37, 65, 66, 562, 2359, 3369
NmeAIII GCCGAG 1 cut(s) 23
NmuCI GTSAC 4 cut(s) 768, 1825, 3065, 3202
NspI RCATGY 1 cut(s) 1646
OliI CACNNNNGTG 1 cut(s) 2147
PaeR7I CTCGAG 1 cut(s) 590
PagI TCATGA 2 cut(s) 93, 808
PdiI GCCGGC 1 cut(s) 118
PdmI GAANNNNTTC 6 cut(s) 360, 576, 1164, 1899, 2994, 3024
PflMI CCANNNNNTGG 2 cut(s) 292, 2114
PfoI TCCNGGA 2 cut(s) 364, 894
PleI GAGTC 6 cut(s) 28, 35, 1085, 1124, 1620, 3353
PmaCI CACGTG 1 cut(s) 2111
PmlI CACGTG 1 cut(s) 2111
PpsI GAGTC 6 cut(s) 28, 35, 1085, 1124, 1620, 3353
Ppu21I YACGTR 1 cut(s) 2111
Psp124BI GAGCTC 1 cut(s) 3464
Psp6I CCWGG 6 cut(s) 59, 364, 894, 1998, 2361, 3700
PspCI CACGTG 1 cut(s) 2111
PspGI CCWGG 6 cut(s) 59, 364, 894, 1998, 2361, 3700
PspN4I GGNNCC 7 cut(s) 6, 37, 65, 66, 562, 2359, 3369
PspOMI GGGCCC 1 cut(s) 64
PspPI GGNCC 7 cut(s) 53, 64, 65, 134, 167, 3079, 3585
PspXI VCTCGAGB 1 cut(s) 590
PstI CTGCAG 2 cut(s) 160, 2434
PstNI CAGNNNCTG 3 cut(s) 292, 2476, 2782
PsuI RGATCY 7 cut(s) 560, 891, 1018, 1036, 1990, 3190, 3367
PvuII CAGCTG 2 cut(s) 2476, 3731
RsaI GTAC 1 cut(s) 1339
RsaNI GTAC 1 cut(s) 1338
RseI CAYNNNNRTG 4 cut(s) 335, 1786, 2147, 2799
SacI GAGCTC 1 cut(s) 3464
Sau96I GGNCC 7 cut(s) 53, 64, 65, 134, 167, 3079, 3585
SchI GAGTC 6 cut(s) 29, 35, 1085, 1124, 1621, 3353
ScrFI CCNGG 7 cut(s) 61, 366, 896, 1668, 2000, 2363, 3702
SduI GDGCHC 5 cut(s) 68, 1314, 2065, 2129, 3464
SfcI CTRYAG 3 cut(s) 156, 934, 2430
Sfr274I CTCGAG 1 cut(s) 590
SgrAI CRCCGGYG 1 cut(s) 116
SinI GGWCC 4 cut(s) 53, 134, 167, 3585
SlaI CTCGAG 1 cut(s) 590
SmiMI CAYNNNNRTG 4 cut(s) 335, 1786, 2147, 2799
SmlI CTYRAG 8 cut(s) 555, 590, 1022, 1154, 2342, 2396, 2414, 3347
SmoI CTYRAG 8 cut(s) 555, 590, 1022, 1154, 2342, 2396, 2414, 3347
SsiI CCGC 9 cut(s) 8, 743, 1169, 1445, 2094, 2860, 3017, 3534, 3641
SspI AATATT 1 cut(s) 3427
SspMI CTAG 9 cut(s) 488, 650, 855, 1208, 1220, 1551, 3038, 3353, 3539
SstI GAGCTC 1 cut(s) 3464
StyD4I CCNGG 7 cut(s) 59, 364, 894, 1666, 1998, 2361, 3700
StyI CCWWGG 7 cut(s) 24, 68, 720, 1289, 1816, 2193, 3588
TaaI ACNGT 7 cut(s) 57, 434, 938, 1624, 1978, 2603, 3600
TaiI ACGT 2 cut(s) 2113, 3458
TaqI TCGA 7 cut(s) 591, 1133, 1326, 2686, 3528, 3633, 3646
TaqII GACCGA 1 cut(s) 122
TatI WGTACW 1 cut(s) 1337
TauI GCSGC 2 cut(s) 10, 3536
TscAI CASTG 9 cut(s) 454, 826, 918, 1981, 2152, 2596, 3333, 3400, 3489
TseFI GTSAC 4 cut(s) 768, 1825, 3065, 3202
TseI GCWGC 9 cut(s) 1562, 1778, 1961, 2237, 2369, 2429, 2473, 3728, 3731
Tsp45I GTSAC 4 cut(s) 768, 1825, 3065, 3202
TspGWI ACGGA 1 cut(s) 1696
TspRI CASTG 9 cut(s) 454, 826, 918, 1981, 2152, 2596, 3333, 3400, 3489
Van91I CCANNNNNTGG 2 cut(s) 292, 2114
Vha464I CTTAAG 1 cut(s) 1022
VneI GTGCAC 1 cut(s) 2061
VpaK11BI GGWCC 4 cut(s) 53, 134, 167, 3585
XagI CCTNNNNNAGG 1 cut(s) 3074
XbaI TCTAGA 1 cut(s) 1219
XceI RCATGY 1 cut(s) 1646
XcmI CCANNNNNNNNNTGG 3 cut(s) 826, 2621, 3414
XhoI CTCGAG 1 cut(s) 590
XmiI GTMKAC 2 cut(s) 933, 2910
XmnI GAANNNNTTC 6 cut(s) 360, 576, 1164, 1899, 2994, 3024
XspI CTAG 9 cut(s) 488, 650, 855, 1208, 1220, 1551, 3038, 3353, 3539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.