Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0014350

Physical Location & Seq

Genomic Coordinates Reverse (-)

10331758 .. 10335772

Gene Structure 4015 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g17981.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1677 bp

ATGCGCTATCGATATCTCACAGCTTCTATAGCTTCCTTGAGAAGCCTATGCTTCCACGAGAGGCCTATGCTTTCAAATTTTCATACATATGGATATGTTGGGTCCCCAATTGAACCCATCTCGCATCTTCCAAAATACAACCTAATCTCACTTTTAGCCACCCCTTGTTCCAATTTGTTTCATTTAGCACGACCCCCAATGCGCCCTTTTTGTTCGGAGAGTGGGATTGAAGGTAATGCTTTAGAAGGAGATGTTGATAAGCTTTTCGAGATTATTATGGATAATGCTCAGCCACATTGTGATATGGAGAAAGCTCTTGACGGGCTTGGTGTCCCATTGACTACTGATTTGGTTGTTCAGGTGCTTCGTAAGTTTCGTTTCGAGGAGAAACTAGCTTTTAGATTTTTCATGTGGGCGGGCCAAAAGGACAACTATGATCATGAAGCTCAGGCGTATAATGATATGATTGATATACTGTCGAGTACCAAGTACAAGGTGAAGCAGTTTCGGATTGTTTGTGATTTATTGGATTATATGAAGAGACATAACAAGAGTAAGGTTCCTGTTGAGGTACTCAGTACGATTTTGAAGCAGTACACGGAGAAGCATCTGACTTATCTGCAGAAATTTGCCAAGAAGAAGAAGATTAGGGTGAAGACGCAGCCGGAGATCAATGCATTGAACTTTCTATTGGATGCCCTGTGCAAGTGCAGTCTTGTACAGGATGCCGAACAGATGTTAAAGAGGGTGAAGAAAAGTATCAAGCCTGATGCAAATACATACAACATTTTGTTTTTCGGTTGGTGTAGAGTTAGGAATCCGAGTAGAGGGATGAGGTTACTGGAAGAGATGATTGAAGTGGGTCACACGCCTGATAATTTCACTTACACTACTGCCATCGATGCCTTTTGCAAAGCAGGGATGGTTACTGAGGCAGCTGAACTTTTTGAGTTCATGCGAACAAAAGGCTCTACAGTTTCTTCTCCTACTGCAAAAACTTATGCAATCATGATTGTGGCGCTTGTGGAGAATGACAGAATGGATGAGTGCTTAAAACTGCTGGGACATATGATAAGTAGTGGTTGCCTTCCTGATGTTTCTACATACAAGGAACTACTCGAGGGGATGTGTTTGGCCGGAAAGGTCGAGGAGGCTTACAAGTTCTTGCAAGAGATGGGAAACAAAGGGTACCCTCCTGATATAGTTACTTATAACTGTTTTCTCAAGGTTCTTTGTGAGAATAAGAAGAGTGATGAAGCACTTAAGCTTTATGGAAGAATGATAGATGTTGGCTGTATGCCTAGTGTACAGACTTATAATATGCTGATTTCAATGTACTTTGAGATGGGTGATCCTGATGGAGCATTCGAGACTTGGCATGAGATGGATAAGAGGGGCTGTGCACAGGACACTGATACTTATTGCATGATGATTGAAGGGCTTTTTGGTTGCAATAAAGTGGAAGATGCGTGTTCTCTCTTGGAAGATGTGGTGAACAAGGGAATGAAATTGCCATATACCAAGTTTGACTCCTTTCTAATGCAGCTTTCTGTAATTGGTGATCTTCAAGCCATCCACAGACTTTCGAGGCATATGAGTAGGTTCTATAATCCTTCTATGGCAAGACGCTTTGCATTGAACCAGAAGCGGAGGAGCGTAAGTTTGAGAGAGCACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

559

Amino Acids

64.03

Weight (kDa)

8.13

Isoelectric Point (pI)

41.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	224 - 270	1.2e-07	PPR repeat family
PPR_long	PF17177	233 - 392	1.8e-07	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	256 - 305	8.4e-12	PPR repeat family
PPR_1	PF12854	288 - 320	4.8e-11	PPR repeat
PPR	PF01535	294 - 323	2.6e-07	PPR repeat
PPR_2	PF13041	295 - 340	2.3e-07	PPR repeat family
PPR_2	PF13041	329 - 377	8.6e-09	PPR repeat family
TPR_24	PF23276	334 - 426	4.5e-07	Fungal tetratrico peptide repeats
PPR_3	PF13812	353 - 412	1.7e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	361 - 392	1.3e-09	PPR repeat
PPR_2	PF13041	364 - 412	6.8e-13	PPR repeat family
PPR_3	PF13812	389 - 446	3.2e-12	Pentatricopeptide repeat domain
PPR_1	PF12854	395 - 427	7.1e-07	PPR repeat
PPR_2	PF13041	399 - 444	7e-14	PPR repeat family
PPR	PF01535	402 - 432	8.9e-06	PPR repeat
PPR_1	PF12854	431 - 462	2e-06	PPR repeat
PPR_2	PF13041	434 - 481	1.7e-13	PPR repeat family
PPR	PF01535	438 - 467	3.6e-06	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0014350)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G73400
fragaria_vesca	FvH4_5g17981
malus_domestica	MD14G1242500.v1.1
prunus_persica	Prupe.5G240200_v2.0.a1
pyrus_communis	pycom06g21040 pycom06g21050
rosa_chinensis	RchiOBHm_Chr7g0177611 RchiOBHm_Chr7g0224181
rosa_laevigata	RLG00000005521
rosa_multiflora	Rmu_ssc0000330.1_g000014
rosa_rugosa	Rorug06G0407800
rosa_samantha	Rh7AG007100 Rh7BG007100 Rh7CG007600 Rh7DG007200 Rh7DG007300
rosa_wichuraiana	Rw7G000610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1212, 1317
Acc65I	GGTACC	1 cut(s)	1188
AccB1I	GGYRCC	1 cut(s)	1188
AciI	CCGC	2 cut(s)	416, 1648
AclWI	GGATC	1 cut(s)	1346
AcoI	YGGCCR	1 cut(s)	1134
AcsI	RAATTY	2 cut(s)	76, 626
AdeI	CACNNNGTG	1 cut(s)	299
AfaI	GTAC	9 cut(s)	484, 491, 573, 580, 596, 720, 1190, 1308, 1337
AfiI	CCNNNNNNNGG	1 cut(s)	827
AflII	CTTAAG	1 cut(s)	1262
AjuI	GAANNNNNNNTTGG	4 cut(s)	674, 706, 1428, 1460
AluBI	AGCT	9 cut(s)	23, 32, 262, 314, 395, 446, 938, 1267, 1546
AluI	AGCT	9 cut(s)	23, 32, 262, 314, 395, 446, 938, 1267, 1546
Alw21I	GWGCWC	2 cut(s)	1405, 1674
Alw26I	GTCTC	2 cut(s)	535, 1364
Alw44I	GTGCAC	1 cut(s)	1401
AlwI	GGATC	1 cut(s)	1346
Ama87I	CYCGRG	1 cut(s)	1118
AoxI	GGCC	3 cut(s)	62, 418, 1134
ApaLI	GTGCAC	1 cut(s)	1401
ApeKI	GCWGC	3 cut(s)	661, 935, 1543
ApoI	RAATTY	2 cut(s)	76, 626
ArsI	GACNNNNNNTTYG	2 cut(s)	331, 363
Asp718I	GGTACC	1 cut(s)	1188
AspLEI	GCGC	3 cut(s)	6, 204, 1021
AspS9I	GGNCC	2 cut(s)	102, 418
AsuHPI	GGTGA	6 cut(s)	508, 664, 760, 1361, 1504, 1571
AvaI	CYCGRG	1 cut(s)	1118
AvaII	GGWCC	1 cut(s)	102
BaeGI	GKGCMC	1 cut(s)	1405
BanI	GGYRCC	1 cut(s)	1188
BauI	CACGAG	1 cut(s)	56
BbsI	GAAGAC	1 cut(s)	662
Bbv12I	GWGCWC	2 cut(s)	1405, 1674
BbvI	GCAGC	3 cut(s)	673, 947, 1555
BccI	CCATC	8 cut(s)	125, 905, 916, 1168, 1339, 1352, 1378, 1580
BclI	TGATCA	1 cut(s)	436
BcoDI	GTCTC	2 cut(s)	535, 1364
BfaI	CTAG	2 cut(s)	392, 1302
BfmI	CTRYAG	3 cut(s)	27, 620, 972
BfoI	RGCGCY	1 cut(s)	1022
BfrI	CTTAAG	1 cut(s)	1262
BisI	GCNGC	3 cut(s)	662, 936, 1544
BlpI	GCTNAGC	1 cut(s)	288
BlsI	GCNGC	3 cut(s)	663, 937, 1545
Bme18I	GGWCC	1 cut(s)	102
BmeT110I	CYCGRG	1 cut(s)	1118
BmgT120I	GGNCC	2 cut(s)	102, 418
BmiI	GGNNCC	4 cut(s)	103, 104, 561, 1190
BmsI	GCATC	7 cut(s)	133, 616, 685, 715, 760, 892, 1456
BpiI	GAAGAC	1 cut(s)	662
Bpu10I	CCTNAGC	1 cut(s)	447
Bpu1102I	GCTNAGC	1 cut(s)	288
BpuEI	CTTGAG	2 cut(s)	58, 1208
Bsa29I	ATCGAT	2 cut(s)	10, 900
BsaXI	ACNNNNNCTCC	2 cut(s)	1645, 1675
Bsc4I	CCNNNNNNNGG	1 cut(s)	827
Bse1I	ACTGG	1 cut(s)	846
BseCI	ATCGAT	2 cut(s)	10, 900
BseGI	GGATG	7 cut(s)	700, 730, 837, 927, 1048, 1131, 1572
BseLI	CCNNNNNNNGG	1 cut(s)	827
BseMII	CTCAG	4 cut(s)	302, 461, 589, 921
BseNI	ACTGG	1 cut(s)	846
BseRI	GAGGAG	3 cut(s)	398, 1163, 1666
BseSI	GKGCMC	1 cut(s)	1405
BseXI	GCAGC	3 cut(s)	673, 947, 1555
BseYI	CCCAGC	1 cut(s)	1060
BsgI	GTGCAG	1 cut(s)	730
BshFI	GGCC	3 cut(s)	64, 420, 1136
BshNI	GGYRCC	1 cut(s)	1188
BshVI	ATCGAT	2 cut(s)	10, 900
BsiHKAI	GWGCWC	2 cut(s)	1405, 1674
BsiHKCI	CYCGRG	1 cut(s)	1118
BsiSI	CCGG	2 cut(s)	665, 1137
BslFI	GGGAC	3 cut(s)	88, 317, 1077
BslI	CCNNNNNNNGG	1 cut(s)	827
BsmAI	GTCTC	2 cut(s)	535, 1364
BsmFI	GGGAC	3 cut(s)	88, 317, 1077
BsmI	GAATGC	1 cut(s)	1364
BsnI	GGCC	3 cut(s)	64, 420, 1136
BsoBI	CYCGRG	1 cut(s)	1118
Bsp1286I	GDGCHC	2 cut(s)	1405, 1674
Bsp1407I	TGTACA	2 cut(s)	718, 1306
Bsp143I	GATC	4 cut(s)	436, 669, 1351, 1561
Bsp1720I	GCTNAGC	1 cut(s)	288
BspACI	CCGC	2 cut(s)	416, 1648
BspANI	GGCC	3 cut(s)	64, 420, 1136
BspCNI	CTCAG	4 cut(s)	301, 460, 588, 922
BspDI	ATCGAT	2 cut(s)	10, 900
BspHI	TCATGA	2 cut(s)	439, 1008
BspLI	GGNNCC	4 cut(s)	103, 104, 561, 1190
BspMAI	CTGCAG	1 cut(s)	624
BspPI	GGATC	1 cut(s)	1346
BspT107I	GGYRCC	1 cut(s)	1188
BspTI	CTTAAG	1 cut(s)	1262
BsrGI	TGTACA	2 cut(s)	718, 1306
BsrI	ACTGG	1 cut(s)	846
BssMI	GATC	4 cut(s)	436, 669, 1351, 1561
BssSI	CACGAG	1 cut(s)	56
Bst2BI	CACGAG	1 cut(s)	56
Bst4CI	ACNGT	3 cut(s)	477, 976, 1217
Bst6I	CTCTTC	3 cut(s)	533, 840, 1241
BstAFI	CTTAAG	1 cut(s)	1262
BstAUI	TGTACA	2 cut(s)	718, 1306
BstC8I	GCNNGC	1 cut(s)	418
BstDEI	CTNAG	4 cut(s)	288, 447, 575, 930
BstF5I	GGATG	7 cut(s)	700, 730, 837, 927, 1048, 1131, 1572
BstH2I	RGCGCY	1 cut(s)	1022
BstHHI	GCGC	3 cut(s)	6, 204, 1021
BstKTI	GATC	4 cut(s)	439, 672, 1354, 1564
BstMAI	GTCTC	2 cut(s)	535, 1364
BstMBI	GATC	4 cut(s)	436, 669, 1351, 1561
BstMWI	GCNNNNNNNGC	2 cut(s)	29, 902
BstSFI	CTRYAG	3 cut(s)	27, 620, 972
BstSLI	GKGCMC	1 cut(s)	1405
BstV1I	GCAGC	3 cut(s)	673, 947, 1555
BstV2I	GAAGAC	1 cut(s)	662
Bsu15I	ATCGAT	2 cut(s)	10, 900
BsuRI	GGCC	3 cut(s)	64, 420, 1136
BsuTUI	ATCGAT	2 cut(s)	10, 900
BtsCI	GGATG	7 cut(s)	700, 730, 837, 927, 1048, 1131, 1572
BtsIMutI	CAGTG	1 cut(s)	1410
Cac8I	GCNNGC	1 cut(s)	418
CciI	TCATGA	2 cut(s)	439, 1008
CfoI	GCGC	3 cut(s)	6, 204, 1021
Cfr13I	GGNCC	2 cut(s)	102, 418
ClaI	ATCGAT	2 cut(s)	10, 900
CseI	GACGC	2 cut(s)	667, 1635
Csp6I	GTAC	9 cut(s)	483, 490, 572, 579, 595, 719, 1189, 1307, 1336
CviAII	CATG	6 cut(s)	409, 440, 955, 1009, 1379, 1426
CviQI	GTAC	9 cut(s)	483, 490, 572, 579, 595, 719, 1189, 1307, 1336
DdeI	CTNAG	4 cut(s)	288, 447, 575, 930
DpnI	GATC	4 cut(s)	438, 671, 1353, 1563
DpnII	GATC	4 cut(s)	436, 669, 1351, 1561
DraIII	CACNNNGTG	1 cut(s)	299
EaeI	YGGCCR	1 cut(s)	1134
Eam1104I	CTCTTC	3 cut(s)	533, 840, 1241
EarI	CTCTTC	3 cut(s)	533, 840, 1241
Eco147I	AGGCCT	1 cut(s)	64
Eco32I	GATATC	1 cut(s)	14
Eco47I	GGWCC	1 cut(s)	102
Eco88I	CYCGRG	1 cut(s)	1118
EcoO109I	RGGNCCY	1 cut(s)	102
EcoRV	GATATC	1 cut(s)	14
EcoT22I	ATGCAT	1 cut(s)	679
FaeI	CATG	6 cut(s)	412, 443, 958, 1012, 1382, 1429
FaqI	GGGAC	3 cut(s)	88, 317, 1077
FatI	CATG	6 cut(s)	408, 439, 954, 1008, 1378, 1425
FauI	CCCGC	1 cut(s)	409
FauNDI	CATATG	3 cut(s)	88, 1068, 1593
FbaI	TGATCA	1 cut(s)	436
Fnu4HI	GCNGC	3 cut(s)	662, 936, 1544
FokI	GGATG	7 cut(s)	707, 737, 844, 934, 1055, 1138, 1559
Fsp4HI	GCNGC	3 cut(s)	662, 936, 1544
FspBI	CTAG	2 cut(s)	392, 1302
GlaI	GCGC	3 cut(s)	5, 203, 1020
GluI	GCNGC	3 cut(s)	662, 936, 1544
GsaI	CCCAGC	1 cut(s)	1064
HaeII	RGCGCY	1 cut(s)	1022
HaeIII	GGCC	3 cut(s)	64, 420, 1136
HapII	CCGG	2 cut(s)	665, 1137
HgaI	GACGC	2 cut(s)	667, 1635
HhaI	GCGC	3 cut(s)	6, 204, 1021
Hin1II	CATG	6 cut(s)	412, 443, 958, 1012, 1382, 1429
Hin6I	GCGC	3 cut(s)	4, 202, 1019
HinP1I	GCGC	3 cut(s)	4, 202, 1019
HindIII	AAGCTT	2 cut(s)	260, 1265
HinfI	GANTC	2 cut(s)	817, 1529
HpaII	CCGG	2 cut(s)	665, 1137
HphI	GGTGA	6 cut(s)	508, 664, 760, 1361, 1504, 1571
Hpy166II	GTNNAC	4 cut(s)	597, 1307, 1403, 1495
Hpy188I	TCNGA	4 cut(s)	217, 510, 612, 822
Hpy188III	TCNNGA	8 cut(s)	268, 317, 440, 1009, 1091, 1196, 1355, 1369
Hpy8I	GTNNAC	4 cut(s)	597, 1307, 1403, 1495
HpyAV	CCTTC	5 cut(s)	224, 239, 1097, 1430, 1623
HpyCH4III	ACNGT	3 cut(s)	477, 976, 1217
HpyF10VI	GCNNNNNNNGC	2 cut(s)	29, 902
HpyF3I	CTNAG	4 cut(s)	288, 447, 575, 930
Hsp92II	CATG	6 cut(s)	412, 443, 958, 1012, 1382, 1429
HspAI	GCGC	3 cut(s)	4, 202, 1019
KflI	GGGWCCC	1 cut(s)	102
KpnI	GGTACC	1 cut(s)	1192
Ksp22I	TGATCA	1 cut(s)	436
Kzo9I	GATC	4 cut(s)	436, 669, 1351, 1561
LmnI	GCTCC	2 cut(s)	1361, 1653
Lsp1109I	GCAGC	3 cut(s)	673, 947, 1555
LweI	GCATC	7 cut(s)	133, 616, 685, 715, 760, 892, 1456
MaeI	CTAG	2 cut(s)	392, 1302
MaeIII	GTNAC	4 cut(s)	837, 863, 925, 1204
MalI	GATC	4 cut(s)	438, 671, 1353, 1563
MboI	GATC	4 cut(s)	436, 669, 1351, 1561
MfeI	CAATTG	1 cut(s)	108
MhlI	GDGCHC	2 cut(s)	1405, 1674
MluCI	AATT	7 cut(s)	76, 108, 172, 626, 877, 1508, 1554
MlyI	GAGTC	1 cut(s)	1523
Mph1103I	ATGCAT	1 cut(s)	679
MseI	TTAA	3 cut(s)	740, 1052, 1263
MslI	CAYNNNNRTG	2 cut(s)	87, 1013
MspA1I	CMGCKG	1 cut(s)	938
MspCI	CTTAAG	1 cut(s)	1262
MspI	CCGG	2 cut(s)	665, 1137
MunI	CAATTG	1 cut(s)	108
Mva1269I	GAATGC	1 cut(s)	1364
MwoI	GCNNNNNNNGC	2 cut(s)	29, 902
NdeI	CATATG	3 cut(s)	88, 1068, 1593
NdeII	GATC	4 cut(s)	436, 669, 1351, 1561
NlaIII	CATG	6 cut(s)	412, 443, 958, 1012, 1382, 1429
NlaIV	GGNNCC	4 cut(s)	103, 104, 561, 1190
NmuCI	GTSAC	1 cut(s)	863
NsiI	ATGCAT	1 cut(s)	679
PaeR7I	CTCGAG	1 cut(s)	1118
PagI	TCATGA	2 cut(s)	439, 1008
PceI	AGGCCT	1 cut(s)	64
PcsI	WCGNNNNNNNCGW	1 cut(s)	373
PctI	GAATGC	1 cut(s)	1364
PfeI	GAWTC	1 cut(s)	817
PkrI	GCNGC	3 cut(s)	663, 937, 1545
PleI	GAGTC	1 cut(s)	1523
PpsI	GAGTC	1 cut(s)	1523
PpuMI	RGGWCCY	1 cut(s)	102
PsiI	TTATAA	2 cut(s)	1212, 1317
Psp5II	RGGWCCY	1 cut(s)	102
PspFI	CCCAGC	1 cut(s)	1060
PspN4I	GGNNCC	4 cut(s)	103, 104, 561, 1190
PspPI	GGNCC	2 cut(s)	102, 418
PspPPI	RGGWCCY	1 cut(s)	102
PspXI	VCTCGAGB	1 cut(s)	1118
PstI	CTGCAG	1 cut(s)	624
PvuII	CAGCTG	1 cut(s)	938
RsaI	GTAC	9 cut(s)	484, 491, 573, 580, 596, 720, 1190, 1308, 1337
RsaNI	GTAC	9 cut(s)	483, 490, 572, 579, 595, 719, 1189, 1307, 1336
RseI	CAYNNNNRTG	2 cut(s)	87, 1013
SaqAI	TTAA	3 cut(s)	740, 1052, 1263
SatI	GCNGC	3 cut(s)	662, 936, 1544
Sau3AI	GATC	4 cut(s)	436, 669, 1351, 1561
Sau96I	GGNCC	2 cut(s)	102, 418
SchI	GAGTC	1 cut(s)	1523
SduI	GDGCHC	2 cut(s)	1405, 1674
SfaNI	GCATC	7 cut(s)	133, 616, 685, 715, 760, 892, 1456
SfcI	CTRYAG	3 cut(s)	27, 620, 972
Sfr274I	CTCGAG	1 cut(s)	1118
SinI	GGWCC	1 cut(s)	102
SlaI	CTCGAG	1 cut(s)	1118
SmiMI	CAYNNNNRTG	2 cut(s)	87, 1013
SmlI	CTYRAG	4 cut(s)	37, 1118, 1223, 1262
SmoI	CTYRAG	4 cut(s)	37, 1118, 1223, 1262
Sse9I	AATT	7 cut(s)	76, 108, 172, 626, 877, 1508, 1554
SseBI	AGGCCT	1 cut(s)	64
SsiI	CCGC	2 cut(s)	416, 1648
SspMI	CTAG	2 cut(s)	392, 1302
StuI	AGGCCT	1 cut(s)	64
TaaI	ACNGT	3 cut(s)	477, 976, 1217
TaqI	TCGA	9 cut(s)	10, 267, 381, 479, 900, 1119, 1146, 1368, 1586
TasI	AATT	7 cut(s)	76, 108, 172, 626, 877, 1508, 1554
TatI	WGTACW	5 cut(s)	489, 594, 718, 1306, 1335
TfiI	GAWTC	1 cut(s)	817
Tru1I	TTAA	3 cut(s)	740, 1052, 1263
Tru9I	TTAA	3 cut(s)	740, 1052, 1263
TscAI	CASTG	1 cut(s)	1417
TseFI	GTSAC	1 cut(s)	863
TseI	GCWGC	3 cut(s)	661, 935, 1543
Tsp45I	GTSAC	1 cut(s)	863
TspDTI	ATGAA	8 cut(s)	71, 170, 397, 456, 551, 943, 1269, 1520
TspGWI	ACGGA	1 cut(s)	614
TspRI	CASTG	1 cut(s)	1417
Vha464I	CTTAAG	1 cut(s)	1262
VneI	GTGCAC	1 cut(s)	1401
VpaK11BI	GGWCC	1 cut(s)	102
XapI	RAATTY	2 cut(s)	76, 626
XhoI	CTCGAG	1 cut(s)	1118
XspI	CTAG	2 cut(s)	392, 1302
Zsp2I	ATGCAT	1 cut(s)	679

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.