Rh7AG007100

Pentatricopeptide repeat-containing protein At1g73400

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0014350
Physical Location & Seq
Forward (+)
490912 .. 492612
1701 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG007100.1

Sequence Viewer

Length: 1701 bp
ATGCGCAATCGATCTCTTACAACTTCTATTGCTTCCTTGAGAAGCCTATGCTTCGCCCAGAGGCCTGTGTCTTATGTTATATATGAATATGTTAGGCCCCCAATCGAACCCATCTCGCATCTTCCAAAATACAACCCATTATTCCCATGTTTACCCGCCCCTTCTTCCAATTTGTTCAAATTAGCGCAACCCTCTCTGCGTCTTTTTTGTTCGGAGAGTGTTACAGTGGGTCAAAGTTTTGAATGTGTTGGGAGTGGTAATGCTTTAGAAGGTGATGTCGATAAGCTTTTCGAGATTATTATGGATAATGCCCAGCCACATTGTGATATGGAGAAAGCTCTCAACCTAGCTGGTGTACCATTGACTACTGATTTGGTTGTTCAGGTGCTTCATAGGTTTCGTTTCGAGGAGAAACTAGCTTTTAGGTTTTTCATGTGGGCGGGCCAAAAGGAAAACTATGATCATGAAGCTCAGGCGTATAATGAGATGATTGATATATTGTCGAGTACCAAGTACAAGGTGAAGCAGTTCCGAATTGTTTGTGATTTGCTGGATTATATGAAGAGGCATAACAAGAGTAAGGTCCCTGTTGAGGTTCTCATAACAATTTTGAAGCAGTACACGGAGAAGCATCTGACCTATCTGCAGAAATTTGCCAAGAAGAAGAAGATAAGGGTGAAGACGCAGCCGGAGATCAATGCCTTGAACTTTCTATTGGATGCCCTGTGCAAGTGCAGTCTTGTACAGGATGCCGAACTGATGTTAAAGAGGGTGAAGAAAAATATCAAGCCTGATGCGAATACATACAACATTTTGTTTTTCGGTTGGTGTAGGGTTAGGAATCCGAGTAGAGGAATGAGGTTACTGGAAGAGATGATTGAAGTGGGTCACACGCCTGATAATTTCACTTACACTACTGCCATTGATGCCTTTTGCAAAGCAGGGATGGTTACCGAGGCAGCTGAACTTTTTGAGTTCATGAGAACAAAAGGTTGTACAATTTCTTCTCCTACTGCAAAAACTTATGCAATCATGATTGTGGCGCTTGTGGAGAATGACAGAATGGAGGAGTGCTTCAAACTTCTGGGACATATGATAAGTAGTGGCTGCCTTCCTGATGTTTCTACATACAAGGAACTACTCGAAGGGATGTGTTTGGCTGGAAAGGTCGAGGAGGCCTACAAGTTCTTGCAGGAGATGGGAAACAAAGGTTACCCTCCCGATATAGTTACTTATAATTGTTTTCTAAAGGTGCTGTGTGAGAATAAAAAGGGTGATGAAGCACTGAAGCTTTACGGGAGAATGATAGATATAGGTTGTATGCCTAGTGTGCAGACTTATAATATGTTGATTACAATGTACTTTGAGATGGGTGATCCTGATGGGGCATTTGAGACTTGGCATGAGATGGATAAGAGGGGCTGTGCACAGGACACTGATACTCATTGCATGATGATTGAAGGGCTTTTCGGTTGCAATAAAGTGGAAGATGCGTGTTGTCTCTTGGAGGATGTGGTGAACAAGGGAATGAAATTGCCATATACCAAGTTTGACTCCTTTCTAATGCAGCTTTCTGTGATTGGTGATCTTCAAGCCATCCACAGACTTTCAGAGCATATGAGAAGGTTCTATAATCCTTCTATGGCAAGACGCTTTGCATTGAACCAGAAGCGGAGGAGCATTAGTATGAGGGAGCACTAA

Protein Analysis

566

Amino Acids

64.97

Weight (kDa)

8.09

Isoelectric Point (pI)

45.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 232 - 278 1.6e-07 PPR repeat family
PPR_long PF17177 242 - 400 3.1e-07 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 268 - 313 1e-10 PPR repeat family
PPR_1 PF12854 296 - 328 4.9e-11 PPR repeat
PPR PF01535 302 - 332 6e-08 PPR repeat
PPR_2 PF13041 303 - 349 5.6e-09 PPR repeat family
PPR_2 PF13041 337 - 385 2.6e-09 PPR repeat family
TPR_24 PF23276 342 - 434 7.2e-07 Fungal tetratrico peptide repeats
PPR_3 PF13812 361 - 420 1.7e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 369 - 400 1.3e-09 PPR repeat
PPR_2 PF13041 372 - 420 6.9e-13 PPR repeat family
PPR_3 PF13812 397 - 454 1.4e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 403 - 435 3e-06 PPR repeat
PPR_2 PF13041 407 - 453 1.2e-13 PPR repeat family
PPR PF01535 410 - 440 5.6e-06 PPR repeat
PPR_1 PF12854 439 - 470 8.3e-06 PPR repeat
PPR_2 PF13041 442 - 489 3.7e-12 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014350)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73400
fragaria_vesca FvH4_5g17981
malus_domestica MD14G1242500.v1.1
prunus_persica Prupe.5G240200_v2.0.a1
pyrus_communis pycom06g21040 pycom06g21050
rosa_chinensis RchiOBHm_Chr7g0177611 RchiOBHm_Chr7g0224181
rosa_laevigata RLG00000005521
rosa_multiflora Rmu_ssc0000330.1_g000014
rosa_rugosa Rorug06G0407800
rosa_samantha Rh7AG007100 Rh7BG007100 Rh7CG007600 Rh7DG007200 Rh7DG007300
rosa_wichuraiana Rw7G000610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1236, 1341
Acc16I TGCGCA 1 cut(s) 5
AciI CCGC 3 cut(s) 156, 440, 1672
AclWI GGATC 1 cut(s) 1370
AcsI RAATTY 1 cut(s) 650
AcuI CTGAAG 1 cut(s) 1307
AdeI CACNNNGTG 1 cut(s) 323
AfaI GTAC 7 cut(s) 357, 508, 515, 620, 744, 997, 1361
AfiI CCNNNNNNNGG 2 cut(s) 592, 851
AgsI TTSAA 9 cut(s) 178, 242, 613, 706, 881, 1078, 1460, 1592, 1663
AjuI GAANNNNNNNTTGG 2 cut(s) 698, 730
AluBI AGCT 8 cut(s) 286, 338, 350, 419, 470, 962, 1291, 1570
AluI AGCT 8 cut(s) 286, 338, 350, 419, 470, 962, 1291, 1570
Alw21I GWGCWC 2 cut(s) 1429, 1698
Alw26I GTCTC 2 cut(s) 1388, 1505
Alw44I GTGCAC 1 cut(s) 1425
AlwI GGATC 1 cut(s) 1370
AoxI GGCC 4 cut(s) 62, 95, 442, 1176
ApaLI GTGCAC 1 cut(s) 1425
ApeKI GCWGC 4 cut(s) 685, 959, 1107, 1567
ApoI RAATTY 1 cut(s) 650
ArsI GACNNNNNNTTYG 2 cut(s) 355, 387
Asp700I GAANNNNTTC 1 cut(s) 527
AspLEI GCGC 3 cut(s) 6, 187, 1045
AspS9I GGNCC 3 cut(s) 96, 442, 583
AsuHPI GGTGA 8 cut(s) 284, 532, 688, 784, 1286, 1385, 1528, 1595
AvaII GGWCC 1 cut(s) 583
BaeGI GKGCMC 1 cut(s) 1429
BarI GAAGNNNNNNTAC 2 cut(s) 988, 1020
BbsI GAAGAC 1 cut(s) 686
Bbv12I GWGCWC 2 cut(s) 1429, 1698
BbvI GCAGC 4 cut(s) 697, 971, 1094, 1579
BccI CCATC 7 cut(s) 119, 940, 1192, 1363, 1376, 1402, 1604
BclI TGATCA 1 cut(s) 460
BcoDI GTCTC 2 cut(s) 1388, 1505
BfaI CTAG 3 cut(s) 347, 416, 1326
BfmI CTRYAG 1 cut(s) 644
BfoI RGCGCY 1 cut(s) 1046
BisI GCNGC 4 cut(s) 686, 960, 1108, 1568
BlsI GCNGC 4 cut(s) 687, 961, 1109, 1569
Bme18I GGWCC 1 cut(s) 583
BmgT120I GGNCC 3 cut(s) 96, 442, 583
BmiI GGNNCC 2 cut(s) 98, 585
BmsI GCATC 7 cut(s) 127, 640, 709, 739, 784, 916, 1480
BpiI GAAGAC 1 cut(s) 686
Bpu10I CCTNAGC 1 cut(s) 471
BpuEI CTTGAG 1 cut(s) 58
Bsa29I ATCGAT 1 cut(s) 10
BsaJI CCNNGG 1 cut(s) 954
BsaXI ACNNNNNCTCC 2 cut(s) 1669, 1699
Bsc4I CCNNNNNNNGG 2 cut(s) 592, 851
Bse1I ACTGG 1 cut(s) 870
Bse3DI GCAATG 1 cut(s) 1444
BseCI ATCGAT 1 cut(s) 10
BseDI CCNNGG 1 cut(s) 954
BseGI GGATG 6 cut(s) 724, 754, 951, 1155, 1516, 1596
BseLI CCNNNNNNNGG 2 cut(s) 592, 851
BseMI GCAATG 1 cut(s) 1444
BseMII CTCAG 1 cut(s) 485
BseNI ACTGG 1 cut(s) 870
BseRI GAGGAG 4 cut(s) 422, 1082, 1187, 1690
BseSI GKGCMC 1 cut(s) 1429
BseXI GCAGC 4 cut(s) 697, 971, 1094, 1579
BseYI CCCAGC 1 cut(s) 312
BsgI GTGCAG 2 cut(s) 754, 1352
BshFI GGCC 4 cut(s) 64, 97, 444, 1178
BshVI ATCGAT 1 cut(s) 10
BsiHKAI GWGCWC 2 cut(s) 1429, 1698
BsiSI CCGG 1 cut(s) 689
BslFI GGGAC 2 cut(s) 569, 1101
BslI CCNNNNNNNGG 2 cut(s) 592, 851
BsmAI GTCTC 2 cut(s) 1388, 1505
BsmFI GGGAC 2 cut(s) 569, 1101
BsnI GGCC 4 cut(s) 64, 97, 444, 1178
Bsp1286I GDGCHC 2 cut(s) 1429, 1698
Bsp1407I TGTACA 2 cut(s) 742, 995
Bsp143I GATC 5 cut(s) 11, 460, 693, 1375, 1585
BspACI CCGC 3 cut(s) 156, 440, 1672
BspANI GGCC 4 cut(s) 64, 97, 444, 1178
BspCNI CTCAG 1 cut(s) 484
BspDI ATCGAT 1 cut(s) 10
BspHI TCATGA 3 cut(s) 463, 978, 1032
BspLI GGNNCC 2 cut(s) 98, 585
BspMAI CTGCAG 1 cut(s) 648
BspPI GGATC 1 cut(s) 1370
BsrDI GCAATG 1 cut(s) 1444
BsrGI TGTACA 2 cut(s) 742, 995
BsrI ACTGG 1 cut(s) 870
BssECI CCNNGG 1 cut(s) 954
BssMI GATC 5 cut(s) 11, 460, 693, 1375, 1585
Bst4CI ACNGT 1 cut(s) 226
Bst6I CTCTTC 2 cut(s) 557, 864
BstAUI TGTACA 2 cut(s) 742, 995
BstC8I GCNNGC 1 cut(s) 442
BstDEI CTNAG 1 cut(s) 471
BstEII GGTNACC 2 cut(s) 949, 1211
BstF5I GGATG 6 cut(s) 724, 754, 951, 1155, 1516, 1596
BstH2I RGCGCY 1 cut(s) 1046
BstHHI GCGC 3 cut(s) 6, 187, 1045
BstKTI GATC 5 cut(s) 14, 463, 696, 1378, 1588
BstMAI GTCTC 2 cut(s) 1388, 1505
BstMBI GATC 5 cut(s) 11, 460, 693, 1375, 1585
BstMWI GCNNNNNNNGC 2 cut(s) 926, 1330
BstPI GGTNACC 2 cut(s) 949, 1211
BstSFI CTRYAG 1 cut(s) 644
BstSLI GKGCMC 1 cut(s) 1429
BstV1I GCAGC 4 cut(s) 697, 971, 1094, 1579
BstV2I GAAGAC 1 cut(s) 686
Bsu15I ATCGAT 1 cut(s) 10
BsuRI GGCC 4 cut(s) 64, 97, 444, 1178
BsuTUI ATCGAT 1 cut(s) 10
BtsCI GGATG 6 cut(s) 724, 754, 951, 1155, 1516, 1596
BtsIMutI CAGTG 3 cut(s) 231, 1283, 1434
Cac8I GCNNGC 1 cut(s) 442
CciI TCATGA 3 cut(s) 463, 978, 1032
CfoI GCGC 3 cut(s) 6, 187, 1045
Cfr13I GGNCC 3 cut(s) 96, 442, 583
ClaI ATCGAT 1 cut(s) 10
CseI GACGC 3 cut(s) 188, 691, 1659
Csp6I GTAC 7 cut(s) 356, 507, 514, 619, 743, 996, 1360
CviAII CATG 7 cut(s) 147, 433, 464, 979, 1033, 1403, 1450
CviQI GTAC 7 cut(s) 356, 507, 514, 619, 743, 996, 1360
DdeI CTNAG 1 cut(s) 471
DpnI GATC 5 cut(s) 13, 462, 695, 1377, 1587
DpnII GATC 5 cut(s) 11, 460, 693, 1375, 1585
DraIII CACNNNGTG 1 cut(s) 323
Eam1104I CTCTTC 2 cut(s) 557, 864
EarI CTCTTC 2 cut(s) 557, 864
Eco147I AGGCCT 2 cut(s) 64, 1178
Eco47I GGWCC 1 cut(s) 583
Eco57I CTGAAG 1 cut(s) 1307
Eco91I GGTNACC 2 cut(s) 949, 1211
EcoO109I RGGNCCY 2 cut(s) 96, 583
EcoO65I GGTNACC 2 cut(s) 949, 1211
FaeI CATG 7 cut(s) 150, 436, 467, 982, 1036, 1406, 1453
FaqI GGGAC 2 cut(s) 569, 1101
FatI CATG 7 cut(s) 146, 432, 463, 978, 1032, 1402, 1449
FauI CCCGC 2 cut(s) 163, 433
FauNDI CATATG 2 cut(s) 1092, 1617
FbaI TGATCA 1 cut(s) 460
Fnu4HI GCNGC 4 cut(s) 686, 960, 1108, 1568
FokI GGATG 6 cut(s) 731, 761, 958, 1162, 1523, 1583
Fsp4HI GCNGC 4 cut(s) 686, 960, 1108, 1568
FspBI CTAG 3 cut(s) 347, 416, 1326
FspI TGCGCA 1 cut(s) 5
GlaI GCGC 3 cut(s) 5, 186, 1044
GluI GCNGC 4 cut(s) 686, 960, 1108, 1568
GsaI CCCAGC 1 cut(s) 316
HaeII RGCGCY 1 cut(s) 1046
HaeIII GGCC 4 cut(s) 64, 97, 444, 1178
HapII CCGG 1 cut(s) 689
HgaI GACGC 3 cut(s) 188, 691, 1659
HhaI GCGC 3 cut(s) 6, 187, 1045
Hin1II CATG 7 cut(s) 150, 436, 467, 982, 1036, 1406, 1453
Hin6I GCGC 3 cut(s) 4, 185, 1043
HinP1I GCGC 3 cut(s) 4, 185, 1043
HindIII AAGCTT 2 cut(s) 284, 1289
HinfI GANTC 2 cut(s) 841, 1553
HpaII CCGG 1 cut(s) 689
HphI GGTGA 8 cut(s) 284, 532, 688, 784, 1286, 1385, 1528, 1595
Hpy166II GTNNAC 5 cut(s) 152, 356, 621, 1427, 1519
Hpy188I TCNGA 5 cut(s) 214, 533, 636, 846, 1612
Hpy188III TCNNGA 7 cut(s) 292, 464, 979, 1033, 1115, 1220, 1379
Hpy8I GTNNAC 5 cut(s) 152, 356, 621, 1427, 1519
HpyAV CCTTC 7 cut(s) 171, 263, 1121, 1139, 1454, 1617, 1647
HpyCH4III ACNGT 1 cut(s) 226
HpyF10VI GCNNNNNNNGC 2 cut(s) 926, 1330
HpyF3I CTNAG 1 cut(s) 471
Hsp92II CATG 7 cut(s) 150, 436, 467, 982, 1036, 1406, 1453
HspAI GCGC 3 cut(s) 4, 185, 1043
Ksp22I TGATCA 1 cut(s) 460
Kzo9I GATC 5 cut(s) 11, 460, 693, 1375, 1585
LmnI GCTCC 2 cut(s) 1677, 1693
Lsp1109I GCAGC 4 cut(s) 697, 971, 1094, 1579
LweI GCATC 7 cut(s) 127, 640, 709, 739, 784, 916, 1480
MaeI CTAG 3 cut(s) 347, 416, 1326
MaeIII GTNAC 6 cut(s) 220, 861, 887, 949, 1211, 1228
MalI GATC 5 cut(s) 13, 462, 695, 1377, 1587
MboI GATC 5 cut(s) 11, 460, 693, 1375, 1585
MhlI GDGCHC 2 cut(s) 1429, 1698
MluCI AATT 9 cut(s) 169, 179, 534, 606, 650, 901, 999, 1237, 1532
MlyI GAGTC 1 cut(s) 1547
MroXI GAANNNNTTC 1 cut(s) 527
MseI TTAA 1 cut(s) 764
MslI CAYNNNNRTG 2 cut(s) 1037, 1685
MspA1I CMGCKG 1 cut(s) 962
MspI CCGG 1 cut(s) 689
MwoI GCNNNNNNNGC 2 cut(s) 926, 1330
NdeI CATATG 2 cut(s) 1092, 1617
NdeII GATC 5 cut(s) 11, 460, 693, 1375, 1585
NlaIII CATG 7 cut(s) 150, 436, 467, 982, 1036, 1406, 1453
NlaIV GGNNCC 2 cut(s) 98, 585
NmuCI GTSAC 1 cut(s) 887
NsbI TGCGCA 1 cut(s) 5
PagI TCATGA 3 cut(s) 463, 978, 1032
PceI AGGCCT 2 cut(s) 64, 1178
PdmI GAANNNNTTC 1 cut(s) 527
PfeI GAWTC 1 cut(s) 841
PkrI GCNGC 4 cut(s) 687, 961, 1109, 1569
PleI GAGTC 1 cut(s) 1547
PpsI GAGTC 1 cut(s) 1547
PpuMI RGGWCCY 1 cut(s) 583
PsiI TTATAA 2 cut(s) 1236, 1341
Psp5II RGGWCCY 1 cut(s) 583
PspEI GGTNACC 2 cut(s) 949, 1211
PspFI CCCAGC 1 cut(s) 312
PspN4I GGNNCC 2 cut(s) 98, 585
PspPI GGNCC 3 cut(s) 96, 442, 583
PspPPI RGGWCCY 1 cut(s) 583
PstI CTGCAG 1 cut(s) 648
PvuII CAGCTG 1 cut(s) 962
RsaI GTAC 7 cut(s) 357, 508, 515, 620, 744, 997, 1361
RsaNI GTAC 7 cut(s) 356, 507, 514, 619, 743, 996, 1360
RseI CAYNNNNRTG 2 cut(s) 1037, 1685
SaqAI TTAA 1 cut(s) 764
SatI GCNGC 4 cut(s) 686, 960, 1108, 1568
Sau3AI GATC 5 cut(s) 11, 460, 693, 1375, 1585
Sau96I GGNCC 3 cut(s) 96, 442, 583
SchI GAGTC 1 cut(s) 1547
SduI GDGCHC 2 cut(s) 1429, 1698
SfaNI GCATC 7 cut(s) 127, 640, 709, 739, 784, 916, 1480
SfcI CTRYAG 1 cut(s) 644
SinI GGWCC 1 cut(s) 583
SmiMI CAYNNNNRTG 2 cut(s) 1037, 1685
SmlI CTYRAG 1 cut(s) 37
SmoI CTYRAG 1 cut(s) 37
Sse9I AATT 9 cut(s) 169, 179, 534, 606, 650, 901, 999, 1237, 1532
SseBI AGGCCT 2 cut(s) 64, 1178
SsiI CCGC 3 cut(s) 156, 440, 1672
SspMI CTAG 3 cut(s) 347, 416, 1326
StuI AGGCCT 2 cut(s) 64, 1178
TaaI ACNGT 1 cut(s) 226
TaqI TCGA 8 cut(s) 10, 105, 279, 291, 405, 503, 1143, 1170
TasI AATT 9 cut(s) 169, 179, 534, 606, 650, 901, 999, 1237, 1532
TatI WGTACW 5 cut(s) 513, 618, 742, 995, 1359
TfiI GAWTC 1 cut(s) 841
Tru1I TTAA 1 cut(s) 764
Tru9I TTAA 1 cut(s) 764
TscAI CASTG 3 cut(s) 231, 1290, 1441
TseFI GTSAC 1 cut(s) 887
TseI GCWGC 4 cut(s) 685, 959, 1107, 1567
Tsp45I GTSAC 1 cut(s) 887
TspDTI ATGAA 8 cut(s) 99, 380, 421, 480, 575, 967, 1293, 1544
TspGWI ACGGA 1 cut(s) 638
TspRI CASTG 3 cut(s) 231, 1290, 1441
VneI GTGCAC 1 cut(s) 1425
VpaK11BI GGWCC 1 cut(s) 583
XapI RAATTY 1 cut(s) 650
XmnI GAANNNNTTC 1 cut(s) 527
XspI CTAG 3 cut(s) 347, 416, 1326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.