RchiOBHm_Chr7g0177611

Pentatricopeptide repeat-containing protein At1g73400

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0014350
Physical Location & Seq
Forward (+)
498786 .. 501225
2440 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ15822

Sequence Viewer

Length: 1698 bp
ATGCGCAATCGATCTCTTACAACTTCTATTGCTTCCTTGAGAAGCCTATGCTTCGCCCAGAGCCCTGTGTCTTATATATATGAATATGTTAGGCCCCCAATCGAACCCATCTCGCATCTTCCAAAATACAACCCATTATTCCCATGTTTACCCGCCCCTTCTTCCAATTTGTTCAGATTAGCGCAACCCTCTCTGCGTCTTTTTTGTTCGGAGAGTGTTACAGTGGGTCAAAGTTTTGAATGTGTTGGGAGTGGTAATGCTTTAGAAGGTGATGTCGATAAGCTTTTCGAGATTATTACGGATAATGCCCAGCCACATTGTGATATGGAGAAAGCTCTCAACCTAGCTGGTGTACCATTGACTACTGATTTGGTTGTTCAGGTGCTTCATAGGTTTCGTTTCGAGGAGAAACTAGCTTTTAGGTTTTTCATGTGGGCGGGCCAAAAGGAAAACTATGATCATGAAGCTCAGGCGTATAATGAGATGATTGATATATTGTCGAGTACCAAGTACAAGGTGAAGCAGTTCCGAATTGTTTGTGATTTACTGGATTATATGAAGAGGCATAACAAGAGTAAGGTTCCTGTTGAGGTTCTCATAACAATTTTGAAGCAGTACACGGAGAAGCATCTGACCTATCTGCAGAAATTTGCCAAGAAGAAGAAGATAAGGGTGAAGACGCAGCCGGAGATCAATGCCTTGAACTTTCTATTGGATGCCCTGTGCAAGTGCAGTCTCGTACAGGATGCCGAACTGATGTTAAAGAGGGTGAAGAAAAATATCAAGCCTGATGCGAATACATACAACATTTTGTTTTTCGGTTGGTGTAGAGTTAGGAATCCGAGTAGAGGAATGAGGTTACTGGAAGAGATGATTGAAGTGGGTCACACGCCTGATAATTTCACTTACACTACTGCCATTGATGCCTTTTGCAAAGCAGGGATGGTTACCGAGGCAGCTGAACTTTTTGAGTTCATGAGAACAAAAGGTTGTACAATTTCTTCTCCTACTGCAAAAACTTATGCAATCATGATTGTGGCGCTTGTGGAGAATGACAGAATGGAGGAGTGCTTCAAACTTCTGGGACATATGATAAGTAGTGGCTGCCTTCCTGATGTTTCTACATACAAGGAACTACTCGAAGGGATGTGTTTGGCTGGAAAGGTCGAGGAGGCCTACAAGTTCTTGCAGGAGATGGGAAACAAAGGTTACCCTCCCGATATAGTTACTTATAATTGTTTTCTAAAGGTGCTATGTGAGAATAAAAAGGGTGATGAGGCACTGAAGCTTTACGGGAGAATGATAGATATAGGTTGTATGCCTAGTGTGCAGACTTATAATATGTTGATTTCAATGTACTTTGAGATGGGTGATCCTGATGGGGCATTTGAGACTTGGCATGAGATGGATAAGAGGGGCTGTGCACAGGACACTGATACTTATTGCATGATGATTGAAGGGCTTTTCGGTTGCAATAAAGTGGAAGATGCGTGTTGTCTCTTGGAGGATGTGGTGAACAAGGGAATGAAATTGCCATATACCAAGTTTGACTCCTTTCTAATGCAGCTTTCTGTGATTGGTGATCTTCAAGCCATCCACAGACTTTCGGAGCATATGAGAAGGTTCTATAATCCTTCTATGGCAAGACGCTTTGCATTGAACCAGAAGCGGAGGAGCATTAGTCTGAGGGAGCACTAA

Protein Analysis

565

Amino Acids

64.79

Weight (kDa)

7.93

Isoelectric Point (pI)

48.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 231 - 277 1.6e-07 PPR repeat family
PPR_long PF17177 241 - 399 3.1e-07 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 267 - 312 1e-10 PPR repeat family
PPR_1 PF12854 295 - 327 4.9e-11 PPR repeat
PPR PF01535 301 - 331 5.9e-08 PPR repeat
PPR_2 PF13041 302 - 348 4.2e-09 PPR repeat family
PPR_2 PF13041 336 - 384 2.6e-09 PPR repeat family
TPR_24 PF23276 341 - 433 7.2e-07 Fungal tetratrico peptide repeats
PPR_3 PF13812 360 - 419 1.7e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 368 - 399 1.3e-09 PPR repeat
PPR_2 PF13041 371 - 419 6.9e-13 PPR repeat family
PPR_3 PF13812 396 - 453 5.5e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 402 - 434 3e-06 PPR repeat
PPR_2 PF13041 406 - 451 1.7e-13 PPR repeat family
PPR PF01535 409 - 439 5.6e-06 PPR repeat
PPR_1 PF12854 438 - 469 2e-06 PPR repeat
PPR_2 PF13041 441 - 488 1.7e-13 PPR repeat family
PPR PF01535 445 - 474 3.6e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014350)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73400
fragaria_vesca FvH4_5g17981
malus_domestica MD14G1242500.v1.1
prunus_persica Prupe.5G240200_v2.0.a1
pyrus_communis pycom06g21040 pycom06g21050
rosa_chinensis RchiOBHm_Chr7g0177611 RchiOBHm_Chr7g0224181
rosa_laevigata RLG00000005521
rosa_multiflora Rmu_ssc0000330.1_g000014
rosa_rugosa Rorug06G0407800
rosa_samantha Rh7AG007100 Rh7BG007100 Rh7CG007600 Rh7DG007200 Rh7DG007300
rosa_wichuraiana Rw7G000610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1233, 1338
Acc16I TGCGCA 1 cut(s) 5
AciI CCGC 3 cut(s) 153, 437, 1669
AclWI GGATC 1 cut(s) 1367
AcsI RAATTY 1 cut(s) 647
AcuI CTGAAG 1 cut(s) 1304
AdeI CACNNNGTG 1 cut(s) 320
AfaI GTAC 7 cut(s) 354, 505, 512, 617, 741, 994, 1358
AfiI CCNNNNNNNGG 1 cut(s) 848
AgsI TTSAA 9 cut(s) 239, 610, 703, 878, 1075, 1353, 1457, 1589, 1660
AjuI GAANNNNNNNTTGG 2 cut(s) 695, 727
AluBI AGCT 8 cut(s) 283, 335, 347, 416, 467, 959, 1288, 1567
AluI AGCT 8 cut(s) 283, 335, 347, 416, 467, 959, 1288, 1567
Alw21I GWGCWC 2 cut(s) 1426, 1695
Alw26I GTCTC 3 cut(s) 740, 1385, 1502
Alw44I GTGCAC 1 cut(s) 1422
AlwI GGATC 1 cut(s) 1367
AoxI GGCC 3 cut(s) 92, 439, 1173
ApaLI GTGCAC 1 cut(s) 1422
ApeKI GCWGC 4 cut(s) 682, 956, 1104, 1564
ApoI RAATTY 1 cut(s) 647
ArsI GACNNNNNNTTYG 2 cut(s) 352, 384
Asp700I GAANNNNTTC 1 cut(s) 524
AspLEI GCGC 3 cut(s) 6, 184, 1042
AspS9I GGNCC 2 cut(s) 93, 439
AsuHPI GGTGA 8 cut(s) 281, 529, 685, 781, 1283, 1382, 1525, 1592
BaeGI GKGCMC 1 cut(s) 1426
BanII GRGCYC 1 cut(s) 65
BarI GAAGNNNNNNTAC 2 cut(s) 985, 1017
BbsI GAAGAC 1 cut(s) 683
Bbv12I GWGCWC 2 cut(s) 1426, 1695
BbvI GCAGC 4 cut(s) 694, 968, 1091, 1576
BccI CCATC 7 cut(s) 116, 937, 1189, 1360, 1373, 1399, 1601
BclI TGATCA 1 cut(s) 457
BcoDI GTCTC 3 cut(s) 740, 1385, 1502
BfaI CTAG 3 cut(s) 344, 413, 1323
BfmI CTRYAG 1 cut(s) 641
BfoI RGCGCY 1 cut(s) 1043
BisI GCNGC 4 cut(s) 683, 957, 1105, 1565
BlsI GCNGC 4 cut(s) 684, 958, 1106, 1566
BmgT120I GGNCC 2 cut(s) 93, 439
BmiI GGNNCC 2 cut(s) 95, 582
BmsI GCATC 7 cut(s) 124, 637, 706, 736, 781, 913, 1477
BpiI GAAGAC 1 cut(s) 683
Bpu10I CCTNAGC 1 cut(s) 468
BpuEI CTTGAG 1 cut(s) 58
Bsa29I ATCGAT 1 cut(s) 10
BsaJI CCNNGG 1 cut(s) 951
BsaXI ACNNNNNCTCC 2 cut(s) 1666, 1696
Bsc4I CCNNNNNNNGG 1 cut(s) 848
Bse1I ACTGG 2 cut(s) 552, 867
BseCI ATCGAT 1 cut(s) 10
BseDI CCNNGG 1 cut(s) 951
BseGI GGATG 6 cut(s) 721, 751, 948, 1152, 1513, 1593
BseLI CCNNNNNNNGG 1 cut(s) 848
BseMII CTCAG 2 cut(s) 482, 1676
BseNI ACTGG 2 cut(s) 552, 867
BseRI GAGGAG 4 cut(s) 419, 1079, 1184, 1687
BseSI GKGCMC 1 cut(s) 1426
BseXI GCAGC 4 cut(s) 694, 968, 1091, 1576
BseYI CCCAGC 1 cut(s) 309
BsgI GTGCAG 2 cut(s) 751, 1349
BshFI GGCC 3 cut(s) 94, 441, 1175
BshVI ATCGAT 1 cut(s) 10
BsiHKAI GWGCWC 2 cut(s) 1426, 1695
BsiSI CCGG 1 cut(s) 686
BslFI GGGAC 1 cut(s) 1098
BslI CCNNNNNNNGG 1 cut(s) 848
BsmAI GTCTC 3 cut(s) 740, 1385, 1502
BsmFI GGGAC 1 cut(s) 1098
BsnI GGCC 3 cut(s) 94, 441, 1175
Bsp1286I GDGCHC 3 cut(s) 65, 1426, 1695
Bsp1407I TGTACA 1 cut(s) 992
Bsp143I GATC 5 cut(s) 11, 457, 690, 1372, 1582
BspACI CCGC 3 cut(s) 153, 437, 1669
BspANI GGCC 3 cut(s) 94, 441, 1175
BspCNI CTCAG 2 cut(s) 481, 1677
BspDI ATCGAT 1 cut(s) 10
BspHI TCATGA 3 cut(s) 460, 975, 1029
BspLI GGNNCC 2 cut(s) 95, 582
BspMAI CTGCAG 1 cut(s) 645
BspPI GGATC 1 cut(s) 1367
BsrGI TGTACA 1 cut(s) 992
BsrI ACTGG 2 cut(s) 552, 867
BssECI CCNNGG 1 cut(s) 951
BssMI GATC 5 cut(s) 11, 457, 690, 1372, 1582
Bst4CI ACNGT 1 cut(s) 223
Bst6I CTCTTC 2 cut(s) 554, 861
BstAUI TGTACA 1 cut(s) 992
BstC8I GCNNGC 1 cut(s) 439
BstDEI CTNAG 2 cut(s) 468, 1685
BstEII GGTNACC 2 cut(s) 946, 1208
BstF5I GGATG 6 cut(s) 721, 751, 948, 1152, 1513, 1593
BstH2I RGCGCY 1 cut(s) 1043
BstHHI GCGC 3 cut(s) 6, 184, 1042
BstKTI GATC 5 cut(s) 14, 460, 693, 1375, 1585
BstMAI GTCTC 3 cut(s) 740, 1385, 1502
BstMBI GATC 5 cut(s) 11, 457, 690, 1372, 1582
BstMWI GCNNNNNNNGC 2 cut(s) 923, 1327
BstPI GGTNACC 2 cut(s) 946, 1208
BstSFI CTRYAG 1 cut(s) 641
BstSLI GKGCMC 1 cut(s) 1426
BstV1I GCAGC 4 cut(s) 694, 968, 1091, 1576
BstV2I GAAGAC 1 cut(s) 683
Bsu15I ATCGAT 1 cut(s) 10
BsuRI GGCC 3 cut(s) 94, 441, 1175
BsuTUI ATCGAT 1 cut(s) 10
BtsCI GGATG 6 cut(s) 721, 751, 948, 1152, 1513, 1593
BtsIMutI CAGTG 3 cut(s) 228, 1280, 1431
Cac8I GCNNGC 1 cut(s) 439
CciI TCATGA 3 cut(s) 460, 975, 1029
CfoI GCGC 3 cut(s) 6, 184, 1042
Cfr13I GGNCC 2 cut(s) 93, 439
ClaI ATCGAT 1 cut(s) 10
CseI GACGC 3 cut(s) 185, 688, 1656
Csp6I GTAC 7 cut(s) 353, 504, 511, 616, 740, 993, 1357
CviAII CATG 7 cut(s) 144, 430, 461, 976, 1030, 1400, 1447
CviQI GTAC 7 cut(s) 353, 504, 511, 616, 740, 993, 1357
DdeI CTNAG 2 cut(s) 468, 1685
DpnI GATC 5 cut(s) 13, 459, 692, 1374, 1584
DpnII GATC 5 cut(s) 11, 457, 690, 1372, 1582
DraIII CACNNNGTG 1 cut(s) 320
Eam1104I CTCTTC 2 cut(s) 554, 861
EarI CTCTTC 2 cut(s) 554, 861
Eco147I AGGCCT 1 cut(s) 1175
Eco24I GRGCYC 1 cut(s) 65
Eco57I CTGAAG 1 cut(s) 1304
Eco91I GGTNACC 2 cut(s) 946, 1208
EcoO109I RGGNCCY 1 cut(s) 93
EcoO65I GGTNACC 2 cut(s) 946, 1208
EcoT38I GRGCYC 1 cut(s) 65
FaeI CATG 7 cut(s) 147, 433, 464, 979, 1033, 1403, 1450
FaqI GGGAC 1 cut(s) 1098
FatI CATG 7 cut(s) 143, 429, 460, 975, 1029, 1399, 1446
FauI CCCGC 2 cut(s) 160, 430
FauNDI CATATG 2 cut(s) 1089, 1614
FbaI TGATCA 1 cut(s) 457
Fnu4HI GCNGC 4 cut(s) 683, 957, 1105, 1565
FokI GGATG 6 cut(s) 728, 758, 955, 1159, 1520, 1580
FriOI GRGCYC 1 cut(s) 65
Fsp4HI GCNGC 4 cut(s) 683, 957, 1105, 1565
FspBI CTAG 3 cut(s) 344, 413, 1323
FspI TGCGCA 1 cut(s) 5
GlaI GCGC 3 cut(s) 5, 183, 1041
GluI GCNGC 4 cut(s) 683, 957, 1105, 1565
GsaI CCCAGC 1 cut(s) 313
HaeII RGCGCY 1 cut(s) 1043
HaeIII GGCC 3 cut(s) 94, 441, 1175
HapII CCGG 1 cut(s) 686
HgaI GACGC 3 cut(s) 185, 688, 1656
HhaI GCGC 3 cut(s) 6, 184, 1042
Hin1II CATG 7 cut(s) 147, 433, 464, 979, 1033, 1403, 1450
Hin6I GCGC 3 cut(s) 4, 182, 1040
HinP1I GCGC 3 cut(s) 4, 182, 1040
HindIII AAGCTT 2 cut(s) 281, 1286
HinfI GANTC 2 cut(s) 838, 1550
HpaII CCGG 1 cut(s) 686
HphI GGTGA 8 cut(s) 281, 529, 685, 781, 1283, 1382, 1525, 1592
Hpy166II GTNNAC 5 cut(s) 149, 353, 618, 1424, 1516
Hpy188I TCNGA 7 cut(s) 176, 211, 530, 633, 843, 1609, 1686
Hpy188III TCNNGA 7 cut(s) 289, 461, 976, 1030, 1112, 1217, 1376
Hpy8I GTNNAC 5 cut(s) 149, 353, 618, 1424, 1516
HpyAV CCTTC 7 cut(s) 168, 260, 1118, 1136, 1451, 1614, 1644
HpyCH4III ACNGT 1 cut(s) 223
HpyF10VI GCNNNNNNNGC 2 cut(s) 923, 1327
HpyF3I CTNAG 2 cut(s) 468, 1685
Hsp92II CATG 7 cut(s) 147, 433, 464, 979, 1033, 1403, 1450
HspAI GCGC 3 cut(s) 4, 182, 1040
Ksp22I TGATCA 1 cut(s) 457
Kzo9I GATC 5 cut(s) 11, 457, 690, 1372, 1582
LmnI GCTCC 3 cut(s) 1609, 1674, 1690
Lsp1109I GCAGC 4 cut(s) 694, 968, 1091, 1576
LweI GCATC 7 cut(s) 124, 637, 706, 736, 781, 913, 1477
MaeI CTAG 3 cut(s) 344, 413, 1323
MaeIII GTNAC 6 cut(s) 217, 858, 884, 946, 1208, 1225
MalI GATC 5 cut(s) 13, 459, 692, 1374, 1584
MboI GATC 5 cut(s) 11, 457, 690, 1372, 1582
MhlI GDGCHC 3 cut(s) 65, 1426, 1695
MluCI AATT 8 cut(s) 166, 531, 603, 647, 898, 996, 1234, 1529
MlyI GAGTC 1 cut(s) 1544
MroXI GAANNNNTTC 1 cut(s) 524
MseI TTAA 1 cut(s) 761
MslI CAYNNNNRTG 1 cut(s) 1034
MspA1I CMGCKG 1 cut(s) 959
MspI CCGG 1 cut(s) 686
MwoI GCNNNNNNNGC 2 cut(s) 923, 1327
NdeI CATATG 2 cut(s) 1089, 1614
NdeII GATC 5 cut(s) 11, 457, 690, 1372, 1582
NlaIII CATG 7 cut(s) 147, 433, 464, 979, 1033, 1403, 1450
NlaIV GGNNCC 2 cut(s) 95, 582
NmuCI GTSAC 1 cut(s) 884
NsbI TGCGCA 1 cut(s) 5
PagI TCATGA 3 cut(s) 460, 975, 1029
PceI AGGCCT 1 cut(s) 1175
PdmI GAANNNNTTC 1 cut(s) 524
PfeI GAWTC 1 cut(s) 838
PkrI GCNGC 4 cut(s) 684, 958, 1106, 1566
PleI GAGTC 1 cut(s) 1544
PpsI GAGTC 1 cut(s) 1544
PsiI TTATAA 2 cut(s) 1233, 1338
PspEI GGTNACC 2 cut(s) 946, 1208
PspFI CCCAGC 1 cut(s) 309
PspN4I GGNNCC 2 cut(s) 95, 582
PspPI GGNCC 2 cut(s) 93, 439
PstI CTGCAG 1 cut(s) 645
PvuII CAGCTG 1 cut(s) 959
RsaI GTAC 7 cut(s) 354, 505, 512, 617, 741, 994, 1358
RsaNI GTAC 7 cut(s) 353, 504, 511, 616, 740, 993, 1357
RseI CAYNNNNRTG 1 cut(s) 1034
SaqAI TTAA 1 cut(s) 761
SatI GCNGC 4 cut(s) 683, 957, 1105, 1565
Sau3AI GATC 5 cut(s) 11, 457, 690, 1372, 1582
Sau96I GGNCC 2 cut(s) 93, 439
SchI GAGTC 1 cut(s) 1544
SduI GDGCHC 3 cut(s) 65, 1426, 1695
SfaNI GCATC 7 cut(s) 124, 637, 706, 736, 781, 913, 1477
SfcI CTRYAG 1 cut(s) 641
SmiMI CAYNNNNRTG 1 cut(s) 1034
SmlI CTYRAG 1 cut(s) 37
SmoI CTYRAG 1 cut(s) 37
Sse9I AATT 8 cut(s) 166, 531, 603, 647, 898, 996, 1234, 1529
SseBI AGGCCT 1 cut(s) 1175
SsiI CCGC 3 cut(s) 153, 437, 1669
SspMI CTAG 3 cut(s) 344, 413, 1323
StuI AGGCCT 1 cut(s) 1175
TaaI ACNGT 1 cut(s) 223
TaqI TCGA 8 cut(s) 10, 102, 276, 288, 402, 500, 1140, 1167
TasI AATT 8 cut(s) 166, 531, 603, 647, 898, 996, 1234, 1529
TatI WGTACW 4 cut(s) 510, 615, 992, 1356
TfiI GAWTC 1 cut(s) 838
Tru1I TTAA 1 cut(s) 761
Tru9I TTAA 1 cut(s) 761
TscAI CASTG 3 cut(s) 228, 1287, 1438
TseFI GTSAC 1 cut(s) 884
TseI GCWGC 4 cut(s) 682, 956, 1104, 1564
Tsp45I GTSAC 1 cut(s) 884
TspDTI ATGAA 7 cut(s) 96, 377, 418, 477, 572, 964, 1541
TspGWI ACGGA 2 cut(s) 314, 635
TspRI CASTG 3 cut(s) 228, 1287, 1438
VneI GTGCAC 1 cut(s) 1422
XapI RAATTY 1 cut(s) 647
XmnI GAANNNNTTC 1 cut(s) 524
XspI CTAG 3 cut(s) 344, 413, 1323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.