FvH4_5g18321

Hydrophobic seed protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0016484
Physical Location & Seq
Reverse (-)
10540996 .. 10541496
501 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g18321.t1

Sequence Viewer

Length: 459 bp
ATGGCTTCCAGAAGCACTGCCTCACTTGCTCTCTTCCTGGTCGTCAATCTCCTCTTCTTCACCATGGTTGCTAACGCCTGTAACACATGCCCTCGACCAAAGCCGAAGCCGAGGCCGAGGCCGAGGCCAAGGCCAACCCCAAGCCCTGGTACCCCTTCCACCCCAAGCCCTGGTACCCCTTCCACCCCTTCTGGCACCGCCACTTGCCCCAGGGATGCCCTAAAATTGGGGATTTGTGCTAATGTCCTCAATAACTTGCTTAACGTCACCATTGGCACACCTCCAGTTCAACCTTGCTGCACTCTCATCCAAGGCCTTGCCGATCTCGAAGCTGCTGTATGCCTCTGCACTGCCATCAGAGCCAGCATTCTCGGCATCAACCTTAACATTCCTATTGCACTCAGCTTGCTTCTTAACGCCTGTGGAAACCAGGTCCCAAGAGGGTTCCAATGTTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrophob_seed

Protein Analysis

153

Amino Acids

15.77

Weight (kDa)

9.44

Isoelectric Point (pI)

42.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrophob_seed PF14547 68 - 152 6.6e-29 Hydrophobic seed protein
Tryp_alpha_amyl PF00234 69 - 151 3e-16 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12090 AT1G62510
fragaria_vesca FvH4_5g18321
malus_domestica MD04G1087300.v1.1 MD06G1062000.v1.1
prunus_persica Prupe.5G073200_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0202791
rosa_laevigata RLG00000003571
rosa_multiflora Rmu_sc0002590.1_g000022 Rmu_sc0003429.1_g000015
rosa_roxburghii Rroxscaffold_3G00254260
rosa_rugosa Rorug07G0073200
rosa_samantha Rh7AG200900
rosa_wichuraiana Rw7G017570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 149, 173
AccB1I GGYRCC 3 cut(s) 149, 173, 194
AccB7I CCANNNNNTGG 2 cut(s) 146, 170
AciI CCGC 1 cut(s) 198
AfaI GTAC 2 cut(s) 151, 175
AfiI CCNNNNNNNGG 3 cut(s) 146, 170, 226
AgsI TTSAA 1 cut(s) 290
AjnI CCWGG 5 cut(s) 36, 145, 169, 209, 429
AluBI AGCT 2 cut(s) 332, 405
AluI AGCT 2 cut(s) 332, 405
AoxI GGCC 5 cut(s) 113, 119, 125, 131, 313
ApeKI GCWGC 2 cut(s) 297, 332
Asp718I GGTACC 2 cut(s) 149, 173
AspS9I GGNCC 1 cut(s) 433
AsuHPI GGTGA 2 cut(s) 52, 259
AvaII GGWCC 1 cut(s) 433
BanI GGYRCC 3 cut(s) 149, 173, 194
BbvI GCAGC 2 cut(s) 284, 319
BccI CCATC 1 cut(s) 362
BciT130I CCWGG 5 cut(s) 38, 147, 171, 211, 431
BfaI CTAG 1 cut(s) 457
BisI GCNGC 2 cut(s) 298, 333
BlsI GCNGC 2 cut(s) 299, 334
Bme1390I CCNGG 5 cut(s) 38, 147, 171, 211, 431
Bme18I GGWCC 1 cut(s) 433
BmgT120I GGNCC 1 cut(s) 433
BmiI GGNNCC 5 cut(s) 151, 175, 196, 435, 446
BmrFI CCNGG 5 cut(s) 38, 147, 171, 211, 431
BmsI GCATC 2 cut(s) 205, 384
BpmI CTGGAG 1 cut(s) 267
Bsc4I CCNNNNNNNGG 3 cut(s) 146, 170, 226
Bse1I ACTGG 1 cut(s) 284
BseBI CCWGG 5 cut(s) 38, 147, 171, 211, 431
BseGI GGATG 2 cut(s) 220, 306
BseLI CCNNNNNNNGG 3 cut(s) 146, 170, 226
BseMII CTCAG 1 cut(s) 415
BseNI ACTGG 1 cut(s) 284
BseRI GAGGAG 1 cut(s) 41
BseXI GCAGC 2 cut(s) 284, 319
BsgI GTGCAG 2 cut(s) 283, 331
BshFI GGCC 5 cut(s) 115, 121, 127, 133, 315
BshNI GGYRCC 3 cut(s) 149, 173, 194
BslFI GGGAC 1 cut(s) 419
BslI CCNNNNNNNGG 3 cut(s) 146, 170, 226
BsmFI GGGAC 1 cut(s) 419
BsmI GAATGC 1 cut(s) 366
BsnI GGCC 5 cut(s) 115, 121, 127, 133, 315
Bsp143I GATC 1 cut(s) 322
Bsp19I CCATGG 1 cut(s) 63
BspACI CCGC 1 cut(s) 198
BspANI GGCC 5 cut(s) 115, 121, 127, 133, 315
BspCNI CTCAG 1 cut(s) 414
BspLI GGNNCC 5 cut(s) 151, 175, 196, 435, 446
BspT107I GGYRCC 3 cut(s) 149, 173, 194
BsrI ACTGG 1 cut(s) 284
BssMI GATC 1 cut(s) 322
BssT1I CCWWGG 3 cut(s) 63, 128, 310
Bst2UI CCWGG 5 cut(s) 38, 147, 171, 211, 431
Bst6I CTCTTC 2 cut(s) 38, 59
BstC8I GCNNGC 2 cut(s) 364, 407
BstDEI CTNAG 1 cut(s) 401
BstDSI CCRYGG 1 cut(s) 63
BstF5I GGATG 2 cut(s) 220, 306
BstKTI GATC 1 cut(s) 325
BstMBI GATC 1 cut(s) 322
BstMWI GCNNNNNNNGC 3 cut(s) 26, 359, 372
BstNI CCWGG 5 cut(s) 38, 147, 171, 211, 431
BstNSI RCATGY 1 cut(s) 90
BstSCI CCNGG 5 cut(s) 36, 145, 169, 209, 429
BstV1I GCAGC 2 cut(s) 284, 319
BsuRI GGCC 5 cut(s) 115, 121, 127, 133, 315
BtgI CCRYGG 1 cut(s) 63
BtsCI GGATG 2 cut(s) 220, 306
BtsI GCAGTG 2 cut(s) 15, 348
BtsIMutI CAGTG 2 cut(s) 15, 348
Cac8I GCNNGC 2 cut(s) 364, 407
Cfr13I GGNCC 1 cut(s) 433
CsiI ACCWGGT 1 cut(s) 429
Csp6I GTAC 2 cut(s) 150, 174
CviAII CATG 2 cut(s) 64, 87
CviQI GTAC 2 cut(s) 150, 174
DdeI CTNAG 1 cut(s) 401
DpnI GATC 1 cut(s) 324
DpnII GATC 1 cut(s) 322
Eam1104I CTCTTC 2 cut(s) 38, 59
EarI CTCTTC 2 cut(s) 38, 59
Eco130I CCWWGG 3 cut(s) 63, 128, 310
Eco147I AGGCCT 1 cut(s) 315
Eco47I GGWCC 1 cut(s) 433
EcoO109I RGGNCCY 1 cut(s) 433
EcoRII CCWGG 5 cut(s) 36, 145, 169, 209, 429
EcoT14I CCWWGG 3 cut(s) 63, 128, 310
ErhI CCWWGG 3 cut(s) 63, 128, 310
FaeI CATG 2 cut(s) 67, 90
FaiI YATR 3 cut(s) 65, 88, 340
FaqI GGGAC 1 cut(s) 419
FatI CATG 2 cut(s) 63, 86
Fnu4HI GCNGC 2 cut(s) 298, 333
FokI GGATG 2 cut(s) 227, 293
Fsp4HI GCNGC 2 cut(s) 298, 333
FspBI CTAG 1 cut(s) 457
GluI GCNGC 2 cut(s) 298, 333
GsuI CTGGAG 1 cut(s) 267
HaeIII GGCC 5 cut(s) 115, 121, 127, 133, 315
Hin1II CATG 2 cut(s) 67, 90
HphI GGTGA 2 cut(s) 52, 259
Hpy188I TCNGA 1 cut(s) 359
Hpy188III TCNNGA 2 cut(s) 9, 326
HpyAV CCTTC 3 cut(s) 165, 189, 198
HpyCH4IV ACGT 1 cut(s) 264
HpyCH4V TGCA 3 cut(s) 300, 348, 398
HpyF10VI GCNNNNNNNGC 3 cut(s) 26, 359, 372
HpyF3I CTNAG 1 cut(s) 401
HpySE526I ACGT 1 cut(s) 264
Hsp92II CATG 2 cut(s) 67, 90
KpnI GGTACC 2 cut(s) 153, 177
Kzo9I GATC 1 cut(s) 322
Lsp1109I GCAGC 2 cut(s) 284, 319
LweI GCATC 2 cut(s) 205, 384
MabI ACCWGGT 1 cut(s) 429
MaeI CTAG 1 cut(s) 457
MaeII ACGT 1 cut(s) 264
MaeIII GTNAC 2 cut(s) 80, 265
MalI GATC 1 cut(s) 324
MboI GATC 1 cut(s) 322
MboII GAAGA 3 cut(s) 25, 46, 49
MluCI AATT 1 cut(s) 224
MseI TTAA 3 cut(s) 261, 384, 414
MspR9I CCNGG 5 cut(s) 38, 147, 171, 211, 431
Mva1269I GAATGC 1 cut(s) 366
MvaI CCWGG 5 cut(s) 38, 147, 171, 211, 431
MwoI GCNNNNNNNGC 3 cut(s) 26, 359, 372
NcoI CCATGG 1 cut(s) 63
NdeII GATC 1 cut(s) 322
NlaIII CATG 2 cut(s) 67, 90
NlaIV GGNNCC 5 cut(s) 151, 175, 196, 435, 446
NmeAIII GCCGAG 4 cut(s) 135, 141, 147, 351
NmuCI GTSAC 1 cut(s) 265
NspI RCATGY 1 cut(s) 90
PasI CCCWGGG 1 cut(s) 210
PceI AGGCCT 1 cut(s) 315
PctI GAATGC 1 cut(s) 366
PflMI CCANNNNNTGG 2 cut(s) 146, 170
PkrI GCNGC 2 cut(s) 299, 334
PpuMI RGGWCCY 1 cut(s) 433
Psp5II RGGWCCY 1 cut(s) 433
Psp6I CCWGG 5 cut(s) 36, 145, 169, 209, 429
PspGI CCWGG 5 cut(s) 36, 145, 169, 209, 429
PspN4I GGNNCC 5 cut(s) 151, 175, 196, 435, 446
PspPI GGNCC 1 cut(s) 433
PspPPI RGGWCCY 1 cut(s) 433
RsaI GTAC 2 cut(s) 151, 175
RsaNI GTAC 2 cut(s) 150, 174
SaqAI TTAA 3 cut(s) 261, 384, 414
SatI GCNGC 2 cut(s) 298, 333
Sau3AI GATC 1 cut(s) 322
Sau96I GGNCC 1 cut(s) 433
ScrFI CCNGG 5 cut(s) 38, 147, 171, 211, 431
SetI ASST 7 cut(s) 267, 283, 295, 334, 384, 407, 435
SexAI ACCWGGT 1 cut(s) 429
SfaNI GCATC 2 cut(s) 205, 384
SinI GGWCC 1 cut(s) 433
Sse9I AATT 1 cut(s) 224
SseBI AGGCCT 1 cut(s) 315
SsiI CCGC 1 cut(s) 198
SspMI CTAG 1 cut(s) 457
StuI AGGCCT 1 cut(s) 315
StyD4I CCNGG 5 cut(s) 36, 145, 169, 209, 429
StyI CCWWGG 3 cut(s) 63, 128, 310
TaiI ACGT 1 cut(s) 267
TaqI TCGA 2 cut(s) 94, 327
TasI AATT 1 cut(s) 224
Tru1I TTAA 3 cut(s) 261, 384, 414
Tru9I TTAA 3 cut(s) 261, 384, 414
TscAI CASTG 2 cut(s) 22, 355
TseFI GTSAC 1 cut(s) 265
TseI GCWGC 2 cut(s) 297, 332
Tsp45I GTSAC 1 cut(s) 265
TspRI CASTG 2 cut(s) 22, 355
Van91I CCANNNNNTGG 2 cut(s) 146, 170
VpaK11BI GGWCC 1 cut(s) 433
XceI RCATGY 1 cut(s) 90
XspI CTAG 1 cut(s) 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.