Rw7G017570

Hydrophobic seed protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0016484
Physical Location & Seq
Forward (+)
18571685 .. 18572748
1064 bp
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UTR
Exon/CDS
Intron
Rw7G017570.1

Sequence Viewer

Length: 522 bp
ATGGCTTCCAGAAGCACTGCCTCACTTGCTCTCTTCCTGGCATTCAATCTCCTCTTCTTCACCATGGTTGCTAATGCCTGCAACACATGCCCTCAGCCGAAGCCGAGGCCTAATCCAAGGCCGAATCCAAGGCCGAATCCGAATCCGAATCCGAATCCGAATCCCAATCCCAATCCCAATCCCAATCCAAATCCGAATCCGAATCCGAATCCGAATCCAAATCCGAATCCGAATCCGAATCCAAACCCCACCCCATCCAGGGGTACTTGCCCTAGGGATACCCTAAAATTGGGGGTTTGTGCTAATGTCCTCAACGGGTTGCTTAACCTCACCATTGGCAATCCTCCAGTTCAACCTTGCTGCAGTCTCATCCAAGGCCTTGCTGACCTTGAAGCTGCTGTATGCCTCTGCACTGCTATCAGAGCCAACATTCTGGGCATCAACCTCAACATTCCGGTTTCTCTCAGCTTGCTTCTTAATGTCTGTGGAAACCGGGTCCCAAGAGGGTTCCAATGTGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrophob_seed

Protein Analysis

173

Amino Acids

18.3

Weight (kDa)

9.3

Isoelectric Point (pI)

27.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trypan_PARP PF05887 29 - 101 4.1e-07 Procyclic acidic repetitive protein (PARP)
LTP_2 PF14368 81 - 172 6.2e-07 Probable lipid transfer
Hydrophob_seed PF14547 89 - 173 4e-29 Hydrophobic seed protein
Tryp_alpha_amyl PF00234 90 - 172 4.9e-17 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12090 AT1G62510
fragaria_vesca FvH4_5g18321
malus_domestica MD04G1087300.v1.1 MD06G1062000.v1.1
prunus_persica Prupe.5G073200_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0202791
rosa_laevigata RLG00000003571
rosa_multiflora Rmu_sc0002590.1_g000022 Rmu_sc0003429.1_g000015
rosa_roxburghii Rroxscaffold_3G00254260
rosa_rugosa Rorug07G0073200
rosa_samantha Rh7AG200900
rosa_wichuraiana Rw7G017570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 1 cut(s) 265
AfiI CCNNNNNNNGG 4 cut(s) 258, 259, 260, 289
AgsI TTSAA 3 cut(s) 46, 353, 392
AjnI CCWGG 2 cut(s) 36, 257
AluBI AGCT 2 cut(s) 395, 468
AluI AGCT 2 cut(s) 395, 468
Alw26I GTCTC 1 cut(s) 371
AoxI GGCC 4 cut(s) 107, 119, 131, 376
ApeKI GCWGC 2 cut(s) 360, 395
AspA2I CCTAGG 1 cut(s) 272
AspS9I GGNCC 1 cut(s) 496
AsuC2I CCSGG 1 cut(s) 494
AsuHPI GGTGA 2 cut(s) 52, 322
AvaII GGWCC 1 cut(s) 496
AvrII CCTAGG 1 cut(s) 272
BbvCI CCTCAGC 1 cut(s) 93
BbvI GCAGC 2 cut(s) 347, 382
BccI CCATC 1 cut(s) 262
BciT130I CCWGG 2 cut(s) 38, 259
BciVI GTATCC 1 cut(s) 271
BcnI CCSGG 1 cut(s) 494
BcoDI GTCTC 1 cut(s) 371
BfaI CTAG 2 cut(s) 273, 520
BfmI CTRYAG 1 cut(s) 361
BfuI GTATCC 1 cut(s) 271
BisI GCNGC 2 cut(s) 361, 396
BlnI CCTAGG 1 cut(s) 272
BlsI GCNGC 2 cut(s) 362, 397
Bme1390I CCNGG 3 cut(s) 38, 259, 494
Bme18I GGWCC 1 cut(s) 496
BmgT120I GGNCC 1 cut(s) 496
BmiI GGNNCC 3 cut(s) 497, 498, 509
BmrFI CCNGG 3 cut(s) 38, 259, 494
BmsI GCATC 1 cut(s) 447
BpmI CTGGAG 1 cut(s) 330
Bpu10I CCTNAGC 1 cut(s) 93
BpuMI CCSGG 1 cut(s) 494
BsaJI CCNNGG 7 cut(s) 63, 104, 116, 128, 258, 272, 373
BsaWI WCCGGW 1 cut(s) 454
Bsc4I CCNNNNNNNGG 4 cut(s) 258, 259, 260, 289
Bse1I ACTGG 1 cut(s) 347
BseBI CCWGG 2 cut(s) 38, 259
BseDI CCNNGG 7 cut(s) 63, 104, 116, 128, 258, 272, 373
BseGI GGATG 2 cut(s) 254, 369
BseLI CCNNNNNNNGG 4 cut(s) 258, 259, 260, 289
BseMII CTCAG 2 cut(s) 107, 478
BseNI ACTGG 1 cut(s) 347
BseRI GAGGAG 1 cut(s) 41
BseXI GCAGC 2 cut(s) 347, 382
BsgI GTGCAG 1 cut(s) 394
BshFI GGCC 4 cut(s) 109, 121, 133, 378
BsiSI CCGG 2 cut(s) 455, 493
BslFI GGGAC 1 cut(s) 482
BslI CCNNNNNNNGG 4 cut(s) 258, 259, 260, 289
BsmAI GTCTC 1 cut(s) 371
BsmFI GGGAC 1 cut(s) 482
BsmI GAATGC 1 cut(s) 41
BsnI GGCC 4 cut(s) 109, 121, 133, 378
Bsp19I CCATGG 1 cut(s) 63
BspANI GGCC 4 cut(s) 109, 121, 133, 378
BspCNI CTCAG 2 cut(s) 106, 477
BspLI GGNNCC 3 cut(s) 497, 498, 509
BspMAI CTGCAG 1 cut(s) 365
BsrI ACTGG 1 cut(s) 347
BssECI CCNNGG 7 cut(s) 63, 104, 116, 128, 258, 272, 373
BssT1I CCWWGG 5 cut(s) 63, 116, 128, 272, 373
Bst2UI CCWGG 2 cut(s) 38, 259
Bst6I CTCTTC 2 cut(s) 38, 59
BstAPI GCANNNNNTGC 1 cut(s) 87
BstC8I GCNNGC 2 cut(s) 79, 470
BstDEI CTNAG 2 cut(s) 93, 464
BstDSI CCRYGG 1 cut(s) 63
BstF5I GGATG 2 cut(s) 254, 369
BstMAI GTCTC 1 cut(s) 371
BstMWI GCNNNNNNNGC 3 cut(s) 26, 87, 422
BstNI CCWGG 2 cut(s) 38, 259
BstNSI RCATGY 1 cut(s) 90
BstSCI CCNGG 3 cut(s) 36, 257, 492
BstSFI CTRYAG 1 cut(s) 361
BstV1I GCAGC 2 cut(s) 347, 382
BstXI CCANNNNNNTGG 1 cut(s) 433
BsuI GTATCC 1 cut(s) 271
BsuRI GGCC 4 cut(s) 109, 121, 133, 378
BtgI CCRYGG 1 cut(s) 63
BtsCI GGATG 2 cut(s) 254, 369
BtsI GCAGTG 2 cut(s) 15, 411
BtsIMutI CAGTG 2 cut(s) 15, 411
Cac8I GCNNGC 2 cut(s) 79, 470
Cfr13I GGNCC 1 cut(s) 496
Csp6I GTAC 1 cut(s) 264
CviAII CATG 2 cut(s) 64, 87
CviQI GTAC 1 cut(s) 264
DdeI CTNAG 2 cut(s) 93, 464
Eam1104I CTCTTC 2 cut(s) 38, 59
EarI CTCTTC 2 cut(s) 38, 59
Eco130I CCWWGG 5 cut(s) 63, 116, 128, 272, 373
Eco147I AGGCCT 2 cut(s) 109, 378
Eco47I GGWCC 1 cut(s) 496
EcoO109I RGGNCCY 1 cut(s) 496
EcoRII CCWGG 2 cut(s) 36, 257
EcoT14I CCWWGG 5 cut(s) 63, 116, 128, 272, 373
ErhI CCWWGG 5 cut(s) 63, 116, 128, 272, 373
FaeI CATG 2 cut(s) 67, 90
FaiI YATR 3 cut(s) 65, 88, 403
FaqI GGGAC 1 cut(s) 482
FatI CATG 2 cut(s) 63, 86
Fnu4HI GCNGC 2 cut(s) 361, 396
FokI GGATG 2 cut(s) 241, 356
Fsp4HI GCNGC 2 cut(s) 361, 396
FspBI CTAG 2 cut(s) 273, 520
GluI GCNGC 2 cut(s) 361, 396
GsuI CTGGAG 1 cut(s) 330
HaeIII GGCC 4 cut(s) 109, 121, 133, 378
HapII CCGG 2 cut(s) 455, 493
Hin1II CATG 2 cut(s) 67, 90
HpaII CCGG 2 cut(s) 455, 493
HphI GGTGA 2 cut(s) 52, 322
Hpy188III TCNNGA 1 cut(s) 9
HpyCH4V TGCA 3 cut(s) 81, 363, 411
HpyF10VI GCNNNNNNNGC 3 cut(s) 26, 87, 422
HpyF3I CTNAG 2 cut(s) 93, 464
Hsp92II CATG 2 cut(s) 67, 90
KflI GGGWCCC 1 cut(s) 496
Lsp1109I GCAGC 2 cut(s) 347, 382
LweI GCATC 1 cut(s) 447
MaeI CTAG 2 cut(s) 273, 520
MboII GAAGA 3 cut(s) 25, 46, 49
MluCI AATT 1 cut(s) 287
MseI TTAA 2 cut(s) 324, 477
MspI CCGG 2 cut(s) 455, 493
MspR9I CCNGG 3 cut(s) 38, 259, 494
Mva1269I GAATGC 1 cut(s) 41
MvaI CCWGG 2 cut(s) 38, 259
MwoI GCNNNNNNNGC 3 cut(s) 26, 87, 422
NciI CCSGG 1 cut(s) 494
NcoI CCATGG 1 cut(s) 63
NlaIII CATG 2 cut(s) 67, 90
NlaIV GGNNCC 3 cut(s) 497, 498, 509
NmeAIII GCCGAG 1 cut(s) 129
NspI RCATGY 1 cut(s) 90
PceI AGGCCT 2 cut(s) 109, 378
PctI GAATGC 1 cut(s) 41
PkrI GCNGC 2 cut(s) 362, 397
PpuMI RGGWCCY 1 cut(s) 496
Psp5II RGGWCCY 1 cut(s) 496
Psp6I CCWGG 2 cut(s) 36, 257
PspGI CCWGG 2 cut(s) 36, 257
PspN4I GGNNCC 3 cut(s) 497, 498, 509
PspPI GGNCC 1 cut(s) 496
PspPPI RGGWCCY 1 cut(s) 496
PstI CTGCAG 1 cut(s) 365
RsaI GTAC 1 cut(s) 265
RsaNI GTAC 1 cut(s) 264
SaqAI TTAA 2 cut(s) 324, 477
SatI GCNGC 2 cut(s) 361, 396
Sau96I GGNCC 1 cut(s) 496
ScrFI CCNGG 3 cut(s) 38, 259, 494
SetI ASST 6 cut(s) 330, 358, 390, 397, 447, 470
SfaNI GCATC 1 cut(s) 447
SfcI CTRYAG 1 cut(s) 361
SinI GGWCC 1 cut(s) 496
Sse9I AATT 1 cut(s) 287
SseBI AGGCCT 2 cut(s) 109, 378
SspMI CTAG 2 cut(s) 273, 520
StuI AGGCCT 2 cut(s) 109, 378
StyD4I CCNGG 3 cut(s) 36, 257, 492
StyI CCWWGG 5 cut(s) 63, 116, 128, 272, 373
TasI AATT 1 cut(s) 287
Tru1I TTAA 2 cut(s) 324, 477
Tru9I TTAA 2 cut(s) 324, 477
TscAI CASTG 2 cut(s) 22, 418
TseI GCWGC 2 cut(s) 360, 395
TspRI CASTG 2 cut(s) 22, 418
VpaK11BI GGWCC 1 cut(s) 496
XceI RCATGY 1 cut(s) 90
XmaJI CCTAGG 1 cut(s) 272
XspI CTAG 2 cut(s) 273, 520
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.