RLG00000003571

Hydrophobic seed protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0016484
Physical Location & Seq
Reverse (-)
50130265 .. 50130774
510 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003571

Sequence Viewer

Length: 510 bp
ATGGCTTCTAGAAGCACTGCCTCACTTGCTCTCTTCCTGGCACTCAATCTCCTCTTCTTCACCATGGTTGCTAATGCCTGCAACACATGCCCTCAGCCGAAGCCGAAGCCGAGGCCGAGGCCGAGGCCGAATCCGAATCCGAATCCGAATCCGAATCCGAATCCGAATCCGAATCCTAATCCTAATCCTAATCCTAATCCGAATCCGAATCCGAATCCGAATCCAACCCCAAACCCCACCCCATCCACGGGTACTTGCCCTAGGGATACCCTAAAATTGGGGGTTTGTGCTAATGTCCTCAACGGGTTGCTTAACCTCACCATTGGCAATCCTCCAGTTAAACCTTGCTGCACTCTCATCCAAGGCCTTGCTGACCTTGAAGCTGCTGTATGCCTTTGCACTGCCATCAGAGCCAACATTCTGGGCATCAACCTCAACATTCCGGTTTCTCTCAGCTTGCTTCTTAATGTCTGTGGAAACCGGGTCCCAAGAGGGTTCCAATGTGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrophob_seed

Protein Analysis

170

Amino Acids

17.8

Weight (kDa)

9.4

Isoelectric Point (pI)

27.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trypan_PARP PF05887 25 - 98 4e-07 Procyclic acidic repetitive protein (PARP)
LTP_2 PF14368 75 - 168 2.2e-07 Probable lipid transfer
Hydrophob_seed PF14547 85 - 169 5.1e-29 Hydrophobic seed protein
Tryp_alpha_amyl PF00234 86 - 168 1.2e-16 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12090 AT1G62510
fragaria_vesca FvH4_5g18321
malus_domestica MD04G1087300.v1.1 MD06G1062000.v1.1
prunus_persica Prupe.5G073200_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0202791
rosa_laevigata RLG00000003571
rosa_multiflora Rmu_sc0002590.1_g000022 Rmu_sc0003429.1_g000015
rosa_roxburghii Rroxscaffold_3G00254260
rosa_rugosa Rorug07G0073200
rosa_samantha Rh7AG200900
rosa_wichuraiana Rw7G017570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 1 cut(s) 253
AfiI CCNNNNNNNGG 3 cut(s) 247, 248, 277
AgsI TTSAA 1 cut(s) 380
AjnI CCWGG 1 cut(s) 36
AluBI AGCT 2 cut(s) 383, 456
AluI AGCT 2 cut(s) 383, 456
AoxI GGCC 4 cut(s) 113, 119, 125, 364
ApeKI GCWGC 2 cut(s) 348, 383
AspA2I CCTAGG 1 cut(s) 260
AspS9I GGNCC 1 cut(s) 484
AsuC2I CCSGG 1 cut(s) 482
AsuHPI GGTGA 2 cut(s) 52, 310
AvaII GGWCC 1 cut(s) 484
AvrII CCTAGG 1 cut(s) 260
BbvCI CCTCAGC 1 cut(s) 93
BbvI GCAGC 2 cut(s) 335, 370
BccI CCATC 2 cut(s) 250, 413
BciT130I CCWGG 1 cut(s) 38
BciVI GTATCC 1 cut(s) 259
BcnI CCSGG 1 cut(s) 482
BfaI CTAG 3 cut(s) 9, 261, 508
BfuI GTATCC 1 cut(s) 259
BisI GCNGC 2 cut(s) 349, 384
BlnI CCTAGG 1 cut(s) 260
BlsI GCNGC 2 cut(s) 350, 385
Bme1390I CCNGG 2 cut(s) 38, 482
Bme18I GGWCC 1 cut(s) 484
BmgT120I GGNCC 1 cut(s) 484
BmiI GGNNCC 3 cut(s) 485, 486, 497
BmrFI CCNGG 2 cut(s) 38, 482
BmsI GCATC 1 cut(s) 435
BpmI CTGGAG 1 cut(s) 318
Bpu10I CCTNAGC 1 cut(s) 93
BpuMI CCSGG 1 cut(s) 482
BsaJI CCNNGG 7 cut(s) 63, 110, 116, 122, 246, 260, 361
BsaWI WCCGGW 1 cut(s) 442
BsaXI ACNNNNNCTCC 2 cut(s) 33, 63
Bsc4I CCNNNNNNNGG 3 cut(s) 247, 248, 277
Bse1I ACTGG 1 cut(s) 335
BseBI CCWGG 1 cut(s) 38
BseDI CCNNGG 7 cut(s) 63, 110, 116, 122, 246, 260, 361
BseGI GGATG 2 cut(s) 242, 357
BseLI CCNNNNNNNGG 3 cut(s) 247, 248, 277
BseMII CTCAG 2 cut(s) 107, 466
BseNI ACTGG 1 cut(s) 335
BseRI GAGGAG 1 cut(s) 41
BseXI GCAGC 2 cut(s) 335, 370
BsgI GTGCAG 1 cut(s) 334
BshFI GGCC 4 cut(s) 115, 121, 127, 366
BsiSI CCGG 2 cut(s) 443, 481
BslFI GGGAC 1 cut(s) 470
BslI CCNNNNNNNGG 3 cut(s) 247, 248, 277
BsmFI GGGAC 1 cut(s) 470
BsnI GGCC 4 cut(s) 115, 121, 127, 366
Bsp19I CCATGG 1 cut(s) 63
BspANI GGCC 4 cut(s) 115, 121, 127, 366
BspCNI CTCAG 2 cut(s) 106, 465
BspLI GGNNCC 3 cut(s) 485, 486, 497
BsrI ACTGG 1 cut(s) 335
BssECI CCNNGG 7 cut(s) 63, 110, 116, 122, 246, 260, 361
BssT1I CCWWGG 3 cut(s) 63, 260, 361
Bst2UI CCWGG 1 cut(s) 38
Bst6I CTCTTC 2 cut(s) 38, 59
BstAPI GCANNNNNTGC 1 cut(s) 87
BstC8I GCNNGC 2 cut(s) 79, 458
BstDEI CTNAG 2 cut(s) 93, 452
BstDSI CCRYGG 2 cut(s) 63, 246
BstF5I GGATG 2 cut(s) 242, 357
BstMWI GCNNNNNNNGC 3 cut(s) 26, 87, 410
BstNI CCWGG 1 cut(s) 38
BstNSI RCATGY 1 cut(s) 90
BstSCI CCNGG 2 cut(s) 36, 480
BstV1I GCAGC 2 cut(s) 335, 370
BstXI CCANNNNNNTGG 1 cut(s) 421
BsuI GTATCC 1 cut(s) 259
BsuRI GGCC 4 cut(s) 115, 121, 127, 366
BtgI CCRYGG 2 cut(s) 63, 246
BtsCI GGATG 2 cut(s) 242, 357
BtsI GCAGTG 2 cut(s) 15, 399
BtsIMutI CAGTG 2 cut(s) 15, 399
Cac8I GCNNGC 2 cut(s) 79, 458
Cfr13I GGNCC 1 cut(s) 484
Csp6I GTAC 1 cut(s) 252
CviAII CATG 2 cut(s) 64, 87
CviQI GTAC 1 cut(s) 252
DdeI CTNAG 2 cut(s) 93, 452
Eam1104I CTCTTC 2 cut(s) 38, 59
EarI CTCTTC 2 cut(s) 38, 59
Eco130I CCWWGG 3 cut(s) 63, 260, 361
Eco147I AGGCCT 1 cut(s) 366
Eco47I GGWCC 1 cut(s) 484
EcoO109I RGGNCCY 1 cut(s) 484
EcoRII CCWGG 1 cut(s) 36
EcoT14I CCWWGG 3 cut(s) 63, 260, 361
ErhI CCWWGG 3 cut(s) 63, 260, 361
FaeI CATG 2 cut(s) 67, 90
FaiI YATR 3 cut(s) 65, 88, 391
FaqI GGGAC 1 cut(s) 470
FatI CATG 2 cut(s) 63, 86
Fnu4HI GCNGC 2 cut(s) 349, 384
FokI GGATG 2 cut(s) 229, 344
Fsp4HI GCNGC 2 cut(s) 349, 384
FspBI CTAG 3 cut(s) 9, 261, 508
GluI GCNGC 2 cut(s) 349, 384
GsuI CTGGAG 1 cut(s) 318
HaeIII GGCC 4 cut(s) 115, 121, 127, 366
HapII CCGG 2 cut(s) 443, 481
Hin1II CATG 2 cut(s) 67, 90
HpaII CCGG 2 cut(s) 443, 481
HphI GGTGA 2 cut(s) 52, 310
Hpy188III TCNNGA 1 cut(s) 9
HpyCH4V TGCA 3 cut(s) 81, 351, 399
HpyF10VI GCNNNNNNNGC 3 cut(s) 26, 87, 410
HpyF3I CTNAG 2 cut(s) 93, 452
Hsp92II CATG 2 cut(s) 67, 90
KflI GGGWCCC 1 cut(s) 484
LpnPI CCDG 7 cut(s) 23, 50, 91, 348, 407, 456, 494
Lsp1109I GCAGC 2 cut(s) 335, 370
LweI GCATC 1 cut(s) 435
MaeI CTAG 3 cut(s) 9, 261, 508
MboII GAAGA 3 cut(s) 25, 46, 49
MluCI AATT 1 cut(s) 275
MmeI TCCRAC 1 cut(s) 248
MseI TTAA 3 cut(s) 312, 339, 465
MspI CCGG 2 cut(s) 443, 481
MspR9I CCNGG 2 cut(s) 38, 482
MvaI CCWGG 1 cut(s) 38
MwoI GCNNNNNNNGC 3 cut(s) 26, 87, 410
NciI CCSGG 1 cut(s) 482
NcoI CCATGG 1 cut(s) 63
NlaIII CATG 2 cut(s) 67, 90
NlaIV GGNNCC 3 cut(s) 485, 486, 497
NmeAIII GCCGAG 3 cut(s) 135, 141, 147
NspI RCATGY 1 cut(s) 90
PceI AGGCCT 1 cut(s) 366
PkrI GCNGC 2 cut(s) 350, 385
PpuMI RGGWCCY 1 cut(s) 484
Psp5II RGGWCCY 1 cut(s) 484
Psp6I CCWGG 1 cut(s) 36
PspGI CCWGG 1 cut(s) 36
PspN4I GGNNCC 3 cut(s) 485, 486, 497
PspPI GGNCC 1 cut(s) 484
PspPPI RGGWCCY 1 cut(s) 484
RsaI GTAC 1 cut(s) 253
RsaNI GTAC 1 cut(s) 252
SaqAI TTAA 3 cut(s) 312, 339, 465
SatI GCNGC 2 cut(s) 349, 384
Sau96I GGNCC 1 cut(s) 484
ScrFI CCNGG 2 cut(s) 38, 482
SetI ASST 6 cut(s) 318, 346, 378, 385, 435, 458
SfaNI GCATC 1 cut(s) 435
SinI GGWCC 1 cut(s) 484
Sse9I AATT 1 cut(s) 275
SseBI AGGCCT 1 cut(s) 366
SspMI CTAG 3 cut(s) 9, 261, 508
StuI AGGCCT 1 cut(s) 366
StyD4I CCNGG 2 cut(s) 36, 480
StyI CCWWGG 3 cut(s) 63, 260, 361
TasI AATT 1 cut(s) 275
Tru1I TTAA 3 cut(s) 312, 339, 465
Tru9I TTAA 3 cut(s) 312, 339, 465
TscAI CASTG 2 cut(s) 22, 406
TseI GCWGC 2 cut(s) 348, 383
TspRI CASTG 2 cut(s) 22, 406
VpaK11BI GGWCC 1 cut(s) 484
XbaI TCTAGA 1 cut(s) 8
XceI RCATGY 1 cut(s) 90
XmaJI CCTAGG 1 cut(s) 260
XspI CTAG 3 cut(s) 9, 261, 508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.