Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0010475

Physical Location & Seq

Genomic Coordinates Forward (+)

12500416 .. 12503318

Gene Structure 2903 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g20900.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1707 bp

ATGTCTTCAGACGACGAAAAAACCCCAGTCAAGGCCATATCAGTGAAGGATAGTGAAAAGAAGAAGAAGAAGAACAAAGAGAAAGACAATGATGCGGACAAAGACTACATGATTAAGCCCCAGAGCACCACTCCCTCCCTGGACACCTCTCAGTGGCCTATTCTTCTCAAGAACTATGACCGCCTCAATGTTCGGACTGGACATTACACACCTCTTCCCTCGGGGTTCTCCCCACTGAAGCGGCCTCTTGCAGAGTATATCAGATATGGTGTTCTCAATCTTGATAAGCCCGCAAACCCATCTTCTCATGAGGTTGTTGCTTGGATCAAACGCATCCTTCGTGTTGAGAAGACTGGTCACAGTGGTACCCTTGATCCTAAGGTTACAGGTAATCTTATTGTCTGTATTGACCGAGCAACACGGCTTGTCAAGTCTCAGCAAGGTGCAGGAAAAGAGTATGTATGTGTTGCTCGCCTACATTCTGCTGTGCCTGAGGTTGCCAAGGTGGCCCGAGCCCTTGAATCCCTCACTGGTGCTGTCTTTCAGAGGCCACCTTTGATATCTGCAGTCAAGAGGCAGCTTAGGATTAGGACCATCTATGAAAGCAAGCTTCTTGAGTATGATGCAGAGAAGCATCTGGTTGTTTTCTGGATTTCCTGTGAGGCAGGTACCTATGTGCGAACACTTTGTGTGCATTTGGGTCTGCTTCTTGGTGTGGGAGGGCATATGCAGGAGCTTAGGAGGGTCAGGTCTGGGACTATGGGTGAGAAAGAGAACATGGTTACCATGCATGATGTCATGGATGCTCAATGGGCTTATGATACTAATAGGGATGAGAGTTATTTGAGGAGGGTTATTATGCCGCTTGAGGTGCTTCTGACCAGTCATAAGAGATTAGTTGTCAAGGATTCGGCAGTGAATGCTATTTGCTATGGTGCCAAGTTGATGATTCCTGGGTTGCTGAGGTTTGAGAATGAAATTGAGGTAGGAGAGGAAGTGGTGCTTATGACTACCAAAGGGGAAGCAATTGCTTTGGGAATTGCTGAGATGACCACTGCTGTTATGGCAACTTGTGATCATGGTGTGGTGGCAAAGATTAAGAGGGTGGTGATGGACAGGGATACTTATCCTAGGAAGTGGGGATTGGGACCAAGGGCTTCCATGAAGAAGAAATTGATTGCAGAGGGAAAATTGGATAAGCATGGGAAGCCTACTGAGAATACCCCTCCAGAATGGGGCCGGAATTTGGTTTTACCGACAGGTGGAGATTCTATTATTGCAAGTGCTGCTGCTGGGCCTGTTGTTGCCACCGAGACGACCACTGTTTCAGAGAAGAAGGACAAGAAAAAGCGCAAACAAGAGGAAATCACTGACAGTCCTATTCCAGTTCCTGCAAAGAAAAGCAAAGGTGAAGAAGTGGCTGAGCCTGAAGTTGAGAAGACAGAAAAGAAGAAGAAGAAAAAAAAGGATAAAGACAATGGTGCTTTGGATTCTTCAGATGGGGAGAAGTCTGTGAAGGTGAAGAAGCACAAAGATAAAGAAGAGGCTGGTTCACCTGAAACTGAAAAGTCTGAAAAGAAGAAAAAGAAAAAAAACAAAGAGGCTGAGGATCATGGTGCCGCTGCCACGGATAATGGGAAGGCTGATGGTGAGGCTGATAAGAGTGAGAAAAAGAAGAAGAAAAAGAAGGATAAAGATGAAGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000154 GO:0000495 GO:0001522 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005732 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009506 GO:0009982 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0016072 GO:0016073 GO:0016074 GO:0016556 GO:0016853 GO:0016866 GO:0022613 GO:0030054 GO:0031118 GO:0031120 GO:0031123 GO:0031126 GO:0031429 GO:0031974 GO:0031981 GO:0032991 GO:0033979 GO:0034470 GO:0034641 GO:0034660 GO:0034964 GO:0040031 GO:0042254 GO:0043144 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043628 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0055044 GO:0070013 GO:0071704 GO:0071840 GO:0072588 GO:0090304 GO:1901360 GO:1902494 GO:1990481 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03008 map03008

Pfam Domains

Protein Families

DKCLD PUA TruB_C_2 TruB_N

Protein Analysis

569

Amino Acids

63.14

Weight (kDa)

9.37

Isoelectric Point (pI)

37.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DKCLD	PF08068	49 - 106	3.4e-31	DKCLD (NUC011) domain
TruB_N	PF01509	110 - 226	8.7e-21	TruB family pseudouridylate synthase (N terminal domain)
TruB_C_2	PF16198	227 - 293	1e-26	tRNA pseudouridylate synthase B C-terminal domain
PUA	PF01472	297 - 370	1.4e-23	PUA domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010475)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G57150
fragaria_vesca	FvH4_5g20900 FvH4_5g20900 FvH4_5g20900
malus_domestica	MD06G1043100.v1.1 MD16G1267700.v1.1 MD16G1267900.v1.1
prunus_persica	Prupe.1G572500_v2.0.a1 Prupe.5G050000_v2.0.a1
pyrus_communis	pycom06g03610 pycom16g23720
rosa_chinensis	RchiOBHm_Chr7g0207911
rosa_laevigata	RLG00000003276
rosa_multiflora	Rmu_sc0006577.1_g000004
rosa_roxburghii	Rroxscaffold_3G00251050
rosa_rugosa	Rorug07G0100200
rosa_samantha	Rh7AG233600 Rh7BG229200 Rh7CG248900 Rh7DG239600
rosa_wichuraiana	Rw0G001290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	656
Acc65I	GGTACC	2 cut(s)	365, 668
AccB1I	GGYRCC	4 cut(s)	365, 668, 935, 1616
AciI	CCGC	6 cut(s)	95, 181, 241, 291, 863, 1620
AclWI	GGATC	3 cut(s)	332, 368, 1617
AcsI	RAATTY	1 cut(s)	1243
AcuI	CTGAAG	3 cut(s)	257, 1449, 1479
AdeI	CACNNNGTG	1 cut(s)	689
AfaI	GTAC	2 cut(s)	367, 670
AfiI	CCNNNNNNNGG	5 cut(s)	31, 153, 1235, 1246, 1262
AgsI	TTSAA	1 cut(s)	521
AjnI	CCWGG	2 cut(s)	138, 952
AjuI	GAANNNNNNNTTGG	2 cut(s)	1127, 1159
AluBI	AGCT	3 cut(s)	580, 610, 736
AluI	AGCT	3 cut(s)	580, 610, 736
Alw21I	GWGCWC	1 cut(s)	128
Alw26I	GTCTC	2 cut(s)	438, 1307
AlwI	GGATC	3 cut(s)	332, 368, 1617
AlwNI	CAGNNNCTG	1 cut(s)	1391
Ama87I	CYCGRG	2 cut(s)	220, 510
AoxI	GGCC	7 cut(s)	33, 155, 242, 507, 548, 1237, 1295
ApeKI	GCWGC	4 cut(s)	577, 1286, 1289, 1622
ApoI	RAATTY	1 cut(s)	1243
Asp718I	GGTACC	2 cut(s)	365, 668
AspA2I	CCTAGG	1 cut(s)	1130
AspLEI	GCGC	1 cut(s)	1353
AspS9I	GGNCC	5 cut(s)	508, 591, 1148, 1237, 1295
AsuHPI	GGTGA	6 cut(s)	776, 1120, 1421, 1531, 1545, 1661
AvaI	CYCGRG	2 cut(s)	220, 510
AvaII	GGWCC	2 cut(s)	591, 1148
AvrII	CCTAGG	1 cut(s)	1130
AxyI	CCTNAGG	2 cut(s)	378, 492
BaeI	ACNNNNGTAYC	4 cut(s)	349, 382, 660, 693
BanI	GGYRCC	4 cut(s)	365, 668, 935, 1616
BanII	GRGCYC	1 cut(s)	517
BbsI	GAAGAC	2 cut(s)	356, 1445
Bbv12I	GWGCWC	1 cut(s)	128
BbvCI	CCTCAGC	2 cut(s)	962, 1605
BbvI	GCAGC	4 cut(s)	589, 1273, 1276, 1609
BccI	CCATC	5 cut(s)	307, 602, 1105, 1493, 1640
BceAI	ACGGC	1 cut(s)	437
BciT130I	CCWGG	2 cut(s)	140, 954
BciVI	GTATCC	1 cut(s)	1114
BclI	TGATCA	1 cut(s)	1075
BcoDI	GTCTC	2 cut(s)	438, 1307
BfaI	CTAG	1 cut(s)	1131
BfmI	CTRYAG	1 cut(s)	564
BfuAI	ACCTGC	1 cut(s)	656
BfuI	GTATCC	1 cut(s)	1114
BglI	GCCNNNNNGGC	1 cut(s)	506
BisI	GCNGC	7 cut(s)	242, 578, 863, 1287, 1290, 1620, 1623
BlnI	CCTAGG	1 cut(s)	1130
BlpI	GCTNAGC	1 cut(s)	1422
BlsI	GCNGC	7 cut(s)	243, 579, 864, 1288, 1291, 1621, 1624
Bme1390I	CCNGG	2 cut(s)	140, 954
Bme18I	GGWCC	2 cut(s)	591, 1148
BmeT110I	CYCGRG	2 cut(s)	220, 510
BmgT120I	GGNCC	5 cut(s)	508, 591, 1148, 1237, 1295
BmiI	GGNNCC	6 cut(s)	367, 670, 937, 1149, 1238, 1618
BmrFI	CCNGG	2 cut(s)	140, 954
BmrI	ACTGGG	1 cut(s)	20
BmsI	GCATC	5 cut(s)	82, 342, 613, 643, 793
BmuI	ACTGGG	1 cut(s)	20
BpiI	GAAGAC	2 cut(s)	356, 1445
BplI	GAGNNNNNCTC	2 cut(s)	115, 147
BpmI	CTGGAG	1 cut(s)	1212
Bpu10I	CCTNAGC	4 cut(s)	581, 737, 962, 1605
Bpu1102I	GCTNAGC	1 cut(s)	1422
BpuEI	CTTGAG	3 cut(s)	152, 635, 887
BsaBI	GATNNNNATC	1 cut(s)	1125
BsaJI	CCNNGG	7 cut(s)	138, 219, 501, 953, 1130, 1151, 1626
BsaXI	ACNNNNNCTCC	2 cut(s)	981, 1011
Bsc4I	CCNNNNNNNGG	5 cut(s)	31, 153, 1235, 1246, 1262
Bse1I	ACTGG	6 cut(s)	26, 202, 358, 535, 882, 1385
Bse21I	CCTNAGG	2 cut(s)	378, 492
Bse8I	GATNNNNATC	1 cut(s)	1125
BseBI	CCWGG	2 cut(s)	140, 954
BseDI	CCNNGG	7 cut(s)	138, 219, 501, 953, 1130, 1151, 1626
BseGI	GGATG	3 cut(s)	333, 808, 838
BseJI	GATNNNNATC	1 cut(s)	1125
BseLI	CCNNNNNNNGG	5 cut(s)	31, 153, 1235, 1246, 1262
BseMII	CTCAG	8 cut(s)	164, 449, 483, 953, 1035, 1206, 1413, 1596
BseNI	ACTGG	6 cut(s)	26, 202, 358, 535, 882, 1385
BseRI	GAGGAG	1 cut(s)	862
BseXI	GCAGC	4 cut(s)	589, 1273, 1276, 1609
BseYI	CCCAGC	1 cut(s)	1292
BsgI	GTGCAG	1 cut(s)	465
BshFI	GGCC	7 cut(s)	35, 157, 244, 509, 550, 1239, 1297
BshNI	GGYRCC	4 cut(s)	365, 668, 935, 1616
BsiHKAI	GWGCWC	1 cut(s)	128
BsiHKCI	CYCGRG	2 cut(s)	220, 510
BsiSI	CCGG	1 cut(s)	1240
BslFI	GGGAC	2 cut(s)	769, 1161
BslI	CCNNNNNNNGG	5 cut(s)	31, 153, 1235, 1246, 1262
BsmAI	GTCTC	2 cut(s)	438, 1307
BsmBI	CGTCTC	1 cut(s)	1307
BsmFI	GGGAC	2 cut(s)	769, 1161
BsmI	GAATGC	1 cut(s)	925
BsnI	GGCC	7 cut(s)	35, 157, 244, 509, 550, 1239, 1297
BsoBI	CYCGRG	2 cut(s)	220, 510
Bsp1286I	GDGCHC	2 cut(s)	128, 517
Bsp143I	GATC	4 cut(s)	324, 373, 1075, 1609
Bsp1720I	GCTNAGC	1 cut(s)	1422
BspACI	CCGC	6 cut(s)	95, 181, 241, 291, 863, 1620
BspANI	GGCC	7 cut(s)	35, 157, 244, 509, 550, 1239, 1297
BspCNI	CTCAG	8 cut(s)	163, 448, 484, 954, 1036, 1207, 1414, 1597
BspHI	TCATGA	1 cut(s)	307
BspLI	GGNNCC	6 cut(s)	367, 670, 937, 1149, 1238, 1618
BspMAI	CTGCAG	1 cut(s)	568
BspMI	ACCTGC	1 cut(s)	656
BspPI	GGATC	3 cut(s)	332, 368, 1617
BspT107I	GGYRCC	4 cut(s)	365, 668, 935, 1616
BsrI	ACTGG	6 cut(s)	26, 202, 358, 535, 882, 1385
BssECI	CCNNGG	7 cut(s)	138, 219, 501, 953, 1130, 1151, 1626
BssMI	GATC	4 cut(s)	324, 373, 1075, 1609
BssT1I	CCWWGG	3 cut(s)	501, 1130, 1151
Bst2UI	CCWGG	2 cut(s)	140, 954
Bst4CI	ACNGT	3 cut(s)	362, 1324, 1376
Bst6I	CTCTTC	2 cut(s)	219, 1536
BstAPI	GCANNNNNTGC	2 cut(s)	920, 1286
BstC8I	GCNNGC	3 cut(s)	291, 472, 608
BstDSI	CCRYGG	1 cut(s)	1626
BstEII	GGTNACC	1 cut(s)	781
BstF5I	GGATG	3 cut(s)	333, 808, 838
BstHHI	GCGC	1 cut(s)	1353
BstKTI	GATC	4 cut(s)	327, 376, 1078, 1612
BstMAI	GTCTC	2 cut(s)	438, 1307
BstMBI	GATC	4 cut(s)	324, 373, 1075, 1609
BstMWI	GCNNNNNNNGC	7 cut(s)	506, 812, 871, 920, 1064, 1207, 1286
BstNI	CCWGG	2 cut(s)	140, 954
BstPI	GGTNACC	1 cut(s)	781
BstSCI	CCNGG	2 cut(s)	138, 952
BstSFI	CTRYAG	1 cut(s)	564
BstV1I	GCAGC	4 cut(s)	589, 1273, 1276, 1609
BstV2I	GAAGAC	2 cut(s)	356, 1445
Bsu36I	CCTNAGG	2 cut(s)	378, 492
BsuI	GTATCC	1 cut(s)	1114
BsuRI	GGCC	7 cut(s)	35, 157, 244, 509, 550, 1239, 1297
BtgI	CCRYGG	1 cut(s)	1626
BtsCI	GGATG	3 cut(s)	333, 808, 838
BtsI	GCAGTG	2 cut(s)	921, 1053
BtsIMutI	CAGTG	9 cut(s)	48, 158, 233, 367, 528, 921, 1053, 1320, 1368
BveI	ACCTGC	1 cut(s)	656
Cac8I	GCNNGC	3 cut(s)	291, 472, 608
CaiI	CAGNNNCTG	1 cut(s)	1391
CciI	TCATGA	1 cut(s)	307
CfoI	GCGC	1 cut(s)	1353
Cfr13I	GGNCC	5 cut(s)	508, 591, 1148, 1237, 1295
Csp6I	GTAC	2 cut(s)	366, 669
CviQI	GTAC	2 cut(s)	366, 669
DpnI	GATC	4 cut(s)	326, 375, 1077, 1611
DpnII	GATC	4 cut(s)	324, 373, 1075, 1609
DraIII	CACNNNGTG	1 cut(s)	689
Eam1104I	CTCTTC	2 cut(s)	219, 1536
EarI	CTCTTC	2 cut(s)	219, 1536
Eco130I	CCWWGG	3 cut(s)	501, 1130, 1151
Eco24I	GRGCYC	1 cut(s)	517
Eco32I	GATATC	1 cut(s)	561
Eco47I	GGWCC	2 cut(s)	591, 1148
Eco57I	CTGAAG	3 cut(s)	257, 1449, 1479
Eco81I	CCTNAGG	2 cut(s)	378, 492
Eco88I	CYCGRG	2 cut(s)	220, 510
Eco91I	GGTNACC	1 cut(s)	781
EcoO65I	GGTNACC	1 cut(s)	781
EcoRII	CCWGG	2 cut(s)	138, 952
EcoRV	GATATC	1 cut(s)	561
EcoT14I	CCWWGG	3 cut(s)	501, 1130, 1151
EcoT22I	ATGCAT	1 cut(s)	792
EcoT38I	GRGCYC	1 cut(s)	517
ErhI	CCWWGG	3 cut(s)	501, 1130, 1151
Esp3I	CGTCTC	1 cut(s)	1307
FalI	AAGNNNNNCTT	2 cut(s)	987, 1019
FaqI	GGGAC	2 cut(s)	769, 1161
FauI	CCCGC	1 cut(s)	298
FauNDI	CATATG	1 cut(s)	726
FbaI	TGATCA	1 cut(s)	1075
Fnu4HI	GCNGC	7 cut(s)	242, 578, 863, 1287, 1290, 1620, 1623
FokI	GGATG	3 cut(s)	320, 815, 845
FriOI	GRGCYC	1 cut(s)	517
Fsp4HI	GCNGC	7 cut(s)	242, 578, 863, 1287, 1290, 1620, 1623
FspBI	CTAG	1 cut(s)	1131
GlaI	GCGC	1 cut(s)	1352
GluI	GCNGC	7 cut(s)	242, 578, 863, 1287, 1290, 1620, 1623
GsaI	CCCAGC	1 cut(s)	1296
GsuI	CTGGAG	1 cut(s)	1212
HaeIII	GGCC	7 cut(s)	35, 157, 244, 509, 550, 1239, 1297
HapII	CCGG	1 cut(s)	1240
HhaI	GCGC	1 cut(s)	1353
Hin6I	GCGC	1 cut(s)	1351
HinP1I	GCGC	1 cut(s)	1351
HindIII	AAGCTT	1 cut(s)	608
HinfI	GANTC	5 cut(s)	521, 908, 949, 1268, 1490
HpaII	CCGG	1 cut(s)	1240
HphI	GGTGA	6 cut(s)	776, 1120, 1421, 1531, 1545, 1661
Hpy166II	GTNNAC	1 cut(s)	1553
Hpy188I	TCNGA	8 cut(s)	10, 195, 263, 546, 879, 1330, 1498, 1573
Hpy188III	TCNNGA	7 cut(s)	169, 281, 308, 571, 614, 649, 1229
Hpy8I	GTNNAC	1 cut(s)	1553
Hpy99I	CGWCG	1 cut(s)	17
HpyAV	CCTTC	6 cut(s)	40, 347, 1330, 1510, 1633, 1681
HpyCH4III	ACNGT	3 cut(s)	362, 1324, 1376
HpyF10VI	GCNNNNNNNGC	7 cut(s)	506, 812, 871, 920, 1064, 1207, 1286
HspAI	GCGC	1 cut(s)	1351
KpnI	GGTACC	2 cut(s)	369, 672
Ksp22I	TGATCA	1 cut(s)	1075
Kzo9I	GATC	4 cut(s)	324, 373, 1075, 1609
LmnI	GCTCC	1 cut(s)	733
Lsp1109I	GCAGC	4 cut(s)	589, 1273, 1276, 1609
LweI	GCATC	5 cut(s)	82, 342, 613, 643, 793
MaeI	CTAG	1 cut(s)	1131
MaeIII	GTNAC	3 cut(s)	356, 382, 781
MalI	GATC	4 cut(s)	326, 375, 1077, 1611
MboI	GATC	4 cut(s)	324, 373, 1075, 1609
MfeI	CAATTG	1 cut(s)	1026
MhlI	GDGCHC	2 cut(s)	128, 517
MluCI	AATT	6 cut(s)	978, 1026, 1038, 1172, 1190, 1243
Mph1103I	ATGCAT	1 cut(s)	792
MseI	TTAA	2 cut(s)	114, 1098
MslI	CAYNNNNRTG	1 cut(s)	41
MspA1I	CMGCKG	1 cut(s)	1622
MspI	CCGG	1 cut(s)	1240
MspR9I	CCNGG	2 cut(s)	140, 954
MunI	CAATTG	1 cut(s)	1026
Mva1269I	GAATGC	1 cut(s)	925
MvaI	CCWGG	2 cut(s)	140, 954
MwoI	GCNNNNNNNGC	7 cut(s)	506, 812, 871, 920, 1064, 1207, 1286
NdeI	CATATG	1 cut(s)	726
NdeII	GATC	4 cut(s)	324, 373, 1075, 1609
NlaIV	GGNNCC	6 cut(s)	367, 670, 937, 1149, 1238, 1618
NmuCI	GTSAC	1 cut(s)	356
NsiI	ATGCAT	1 cut(s)	792
PagI	TCATGA	1 cut(s)	307
PcsI	WCGNNNNNNNCGW	1 cut(s)	337
PctI	GAATGC	1 cut(s)	925
PfeI	GAWTC	5 cut(s)	521, 908, 949, 1268, 1490
PkrI	GCNGC	7 cut(s)	243, 579, 864, 1288, 1291, 1621, 1624
Psp6I	CCWGG	2 cut(s)	138, 952
PspEI	GGTNACC	1 cut(s)	781
PspFI	CCCAGC	1 cut(s)	1292
PspGI	CCWGG	2 cut(s)	138, 952
PspN4I	GGNNCC	6 cut(s)	367, 670, 937, 1149, 1238, 1618
PspPI	GGNCC	5 cut(s)	508, 591, 1148, 1237, 1295
PstI	CTGCAG	1 cut(s)	568
PstNI	CAGNNNCTG	1 cut(s)	1391
RsaI	GTAC	2 cut(s)	367, 670
RsaNI	GTAC	2 cut(s)	366, 669
RseI	CAYNNNNRTG	1 cut(s)	41
SaqAI	TTAA	2 cut(s)	114, 1098
SatI	GCNGC	7 cut(s)	242, 578, 863, 1287, 1290, 1620, 1623
Sau3AI	GATC	4 cut(s)	324, 373, 1075, 1609
Sau96I	GGNCC	5 cut(s)	508, 591, 1148, 1237, 1295
ScrFI	CCNGG	2 cut(s)	140, 954
SduI	GDGCHC	2 cut(s)	128, 517
SfaNI	GCATC	5 cut(s)	82, 342, 613, 643, 793
SfcI	CTRYAG	1 cut(s)	564
SinI	GGWCC	2 cut(s)	591, 1148
SmiMI	CAYNNNNRTG	1 cut(s)	41
SmlI	CTYRAG	3 cut(s)	167, 614, 866
SmoI	CTYRAG	3 cut(s)	167, 614, 866
Sse9I	AATT	6 cut(s)	978, 1026, 1038, 1172, 1190, 1243
SsiI	CCGC	6 cut(s)	95, 181, 241, 291, 863, 1620
SspMI	CTAG	1 cut(s)	1131
StyD4I	CCNGG	2 cut(s)	138, 952
StyI	CCWWGG	3 cut(s)	501, 1130, 1151
TaaI	ACNGT	3 cut(s)	362, 1324, 1376
TaqII	GACCGA	1 cut(s)	426
TasI	AATT	6 cut(s)	978, 1026, 1038, 1172, 1190, 1243
TauI	GCSGC	3 cut(s)	244, 865, 1622
TfiI	GAWTC	5 cut(s)	521, 908, 949, 1268, 1490
Tru1I	TTAA	2 cut(s)	114, 1098
Tru9I	TTAA	2 cut(s)	114, 1098
TscAI	CASTG	9 cut(s)	48, 158, 240, 367, 535, 921, 1060, 1327, 1375
TseFI	GTSAC	1 cut(s)	356
TseI	GCWGC	4 cut(s)	577, 1286, 1289, 1622
Tsp45I	GTSAC	1 cut(s)	356
TspDTI	ATGAA	3 cut(s)	615, 990, 1178
TspGWI	ACGGA	1 cut(s)	1643
TspRI	CASTG	9 cut(s)	48, 158, 240, 367, 535, 921, 1060, 1327, 1375
VpaK11BI	GGWCC	2 cut(s)	591, 1148
XapI	RAATTY	1 cut(s)	1243
XcmI	CCANNNNNNNNNTGG	2 cut(s)	136, 1060
XmaJI	CCTAGG	1 cut(s)	1130
XspI	CTAG	1 cut(s)	1131
Zsp2I	ATGCAT	1 cut(s)	792

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.