Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0010475

Physical Location & Seq

Genomic Coordinates Forward (+)

46472918 .. 46475544

Gene Structure 2627 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000003276

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1893 bp

ATGCTGAAACGCCAGCATTACAGAAACCAGGCCATGGGCCCAAAGAGAGGCGAAGTGAGGAGTGAGAGTCAGAACCCTCTAGGTGGCCGCCCTCTCGCTCAACTGTCCAAATCCAAAAGGGTTTTGCTCCCAGCCGAAATCAAACTACCAAGAAAAATGTCTTCAGACGACGAAAAAACCCCAGTCAAGGCCATATCAGTGAAGGATAGTGAAAAGAAGAAGAAGAAGCACAGAGAGAAAGACAATGATGTGGACCAAGACTACATGATCAAGCCCCAGAGCTCGACTCCCTCCCTGGACACATCTCAGTGGCCCATTCTTCTCAAGAACTACGACCGCCTCAATGTTCGAACTGGACATTACACACCTCTTCCCTCGGGGTACTCCCCGCTCAAGCGGCCTCTTGCAGAGTATATCAGATATGGTGTTCTCAATCTTGACAAGCCCGCAAACCCCTCTTCCCACGAGGTTGTTGCTTGGATTAAACGCATCCTTCGTGTTGAGAAGACTGGTCACAGTGGGACTCTTGATCCTAAGGTTACAGGTAATCTCATTGTCTGTATTGACCGAGCAACACGACTTGTCAAGTCTCAGCAAGGTGCAGGAAAAGAGTATGTCTGTGTTGCACGCCTGCATTCAGCTGTGCCTGATGTTGCCAAGGTGGCCCGGGCACTTGAATCCCTCACTGGCGCTGTCTTTCAGAGGCCACCTTTGATATCTGCAGTCAAGAGGCAGCTTAGGATTAGGACCATCTATGAAAGCAAGCTTCTTGAGTATGATGCGGAGAAGCATCTGGTTGTTTTCTGGATTTCCTGTGAGGCAGGTACCTATGTGCGAACACTGTGTGTGCATTTGGGTCTGCTTCTTGGTGTAGGAGGGCATATGCAGGAGCTTAGGAGGGTCAGATCTGGGACTATGGGTGAGAAAGAGAACATGGTTACCATGCATGATGTCATGGATGCTCAATGGGTTTATGATACTAATAGGGATGAGAGTTATTTGAGGAGGGTTATTATGCCACTTGAGGTGCTTCTGACAAGTCATAAGAGATTAGTTGTCAAGGACTCAGCAGTGAATGCCATTTGCTATGGTGCCAAGTTGATGATTCCTGGGCTACTGAGGTTTGAGAATGAAATTGAGGTAGGAGAGGAAGTTGTGCTAATGACTACCAAAGGTGAAGCGATTGCTTTGGGAATTGCTGAGATGACCACTGCTGTGATGGCAACTTGTGATCATGGTGTGGTGGCAAAGATTAAGAGGGTGGTGATGGATAGGGATACTTATCCTAGGAAGTGGGGATTGGGGCCAAGGGCATCCATGAAGAAGAAATTGATTGCAGAAGGGAAATTGGATAAGCATGGGAAGCCTACTGAGAATACTCCTCCAGAATGGGGCCGGAATTTGGTTTTACCGACAGGTGGAGATTCTGTTATTGCAAGTGCTGCTGCTGAGCTTGCTGCTGCACCTGCTGTTGCCACTGAGACAACTTCTGTTTCAGAGAAGAAGGAGAAGAAAAAACGCAAACAAGAGGAAATAACTGACAGTCCTGTTCCAGTTCCTGCAAAGAAAAGCAAAGGTGAAGAAGTAGCTGAGGTTGAAGTTGAGAAGACAGAAAAGAAGAAGAAGAAAAAGAAAAAGGATAAAGACAATGGTGTTTTGGATTCTTCAGATGGGGAGAAGTCTGTGAAGGTGAAGAAGCACAAAGATAAAGAAGAAGCTGGTTCACCTCAAACTGAAAAGTCTGAAAAGAAGAAAAAGAAGAAAAACAAAGAGGCTGAGGATCATGGTGCTGATGCTGCCACTGATAATGGGAAGGGTGATGGTGAGGCTGATAAGAGTGAGAAAAAGAAGAAGAAGAAGAAAAAGGATAAATCAGATGCTGACGAAGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000154 GO:0000495 GO:0001522 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005732 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009506 GO:0009982 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0016072 GO:0016073 GO:0016074 GO:0016556 GO:0016853 GO:0016866 GO:0022613 GO:0030054 GO:0031118 GO:0031120 GO:0031123 GO:0031126 GO:0031429 GO:0031974 GO:0031981 GO:0032991 GO:0033979 GO:0034470 GO:0034641 GO:0034660 GO:0034964 GO:0040031 GO:0042254 GO:0043144 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043628 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0055044 GO:0070013 GO:0071704 GO:0071840 GO:0072588 GO:0090304 GO:1901360 GO:1902494 GO:1990481 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03008 map03008

Pfam Domains

Protein Families

DKCLD PUA TruB_C_2 TruB_N

Protein Analysis

631

Amino Acids

70.17

Weight (kDa)

9.54

Isoelectric Point (pI)

40.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DKCLD	PF08068	101 - 158	5.7e-31	DKCLD (NUC011) domain
TruB_N	PF01509	162 - 278	1.1e-20	TruB family pseudouridylate synthase (N terminal domain)
TruB_C_2	PF16198	279 - 345	7.4e-27	tRNA pseudouridylate synthase B C-terminal domain
PUA	PF01472	349 - 422	1.6e-23	PUA domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0010475)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G57150
fragaria_vesca	FvH4_5g20900 FvH4_5g20900 FvH4_5g20900
malus_domestica	MD06G1043100.v1.1 MD16G1267700.v1.1 MD16G1267900.v1.1
prunus_persica	Prupe.1G572500_v2.0.a1 Prupe.5G050000_v2.0.a1
pyrus_communis	pycom06g03610 pycom16g23720
rosa_chinensis	RchiOBHm_Chr7g0207911
rosa_laevigata	RLG00000003276
rosa_multiflora	Rmu_sc0006577.1_g000004
rosa_roxburghii	Rroxscaffold_3G00251050
rosa_rugosa	Rorug07G0100200
rosa_samantha	Rh7AG233600 Rh7BG229200 Rh7CG248900 Rh7DG239600
rosa_wichuraiana	Rw0G001290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1474
Acc36I	ACCTGC	2 cut(s)	812, 1474
Acc65I	GGTACC	1 cut(s)	824
AccB1I	GGYRCC	2 cut(s)	824, 1091
AccB7I	CCANNNNNTGG	1 cut(s)	34
AccBSI	CCGCTC	1 cut(s)	391
AciI	CCGC	6 cut(s)	88, 337, 389, 397, 447, 782
AclWI	GGATC	2 cut(s)	524, 1788
AcoI	YGGCCR	1 cut(s)	85
AcsI	RAATTY	1 cut(s)	1399
AcuI	CTGAAG	2 cut(s)	147, 1650
AdeI	CACNNNGTG	1 cut(s)	845
AfaI	GTAC	2 cut(s)	383, 826
AfiI	CCNNNNNNNGG	7 cut(s)	34, 47, 83, 187, 1391, 1402, 1418
AgsI	TTSAA	2 cut(s)	677, 1598
AjnI	CCWGG	3 cut(s)	27, 294, 1108
AjuI	GAANNNNNNNTTGG	2 cut(s)	1283, 1315
AleI	CACNNNNGTG	1 cut(s)	1214
AluBI	AGCT	8 cut(s)	282, 641, 736, 766, 892, 1453, 1589, 1718
AluI	AGCT	8 cut(s)	282, 641, 736, 766, 892, 1453, 1589, 1718
Alw21I	GWGCWC	1 cut(s)	284
Alw26I	GTCTC	2 cut(s)	594, 1475
AlwI	GGATC	2 cut(s)	524, 1788
AlwNI	CAGNNNCTG	2 cut(s)	1559, 1880
Ama87I	CYCGRG	2 cut(s)	376, 666
ApaI	GGGCCC	1 cut(s)	41
ApeKI	GCWGC	6 cut(s)	733, 1442, 1445, 1457, 1460, 1796
ApoI	RAATTY	1 cut(s)	1399
Asp718I	GGTACC	1 cut(s)	824
AspA2I	CCTAGG	1 cut(s)	1286
AspLEI	GCGC	1 cut(s)	692
AspS9I	GGNCC	8 cut(s)	37, 38, 253, 312, 664, 747, 1304, 1393
AsuC2I	CCSGG	2 cut(s)	667, 668
AsuHPI	GGTGA	8 cut(s)	932, 1187, 1276, 1589, 1702, 1716, 1829, 1835
AsuII	TTCGAA	1 cut(s)	349
AvaI	CYCGRG	2 cut(s)	376, 666
AvaII	GGWCC	2 cut(s)	253, 747
AvrII	CCTAGG	1 cut(s)	1286
AxyI	CCTNAGG	1 cut(s)	534
BaeGI	GKGCMC	2 cut(s)	41, 673
BaeI	ACNNNNGTAYC	2 cut(s)	816, 849
BanI	GGYRCC	2 cut(s)	824, 1091
BanII	GRGCYC	2 cut(s)	41, 284
BauI	CACGAG	1 cut(s)	464
BbsI	GAAGAC	3 cut(s)	153, 512, 1613
Bbv12I	GWGCWC	1 cut(s)	284
BbvCI	CCTCAGC	2 cut(s)	1590, 1776
BbvI	GCAGC	6 cut(s)	745, 1429, 1432, 1444, 1447, 1783
BccI	CCATC	5 cut(s)	758, 1213, 1261, 1664, 1814
BcgI	CGANNNNNNTGC	2 cut(s)	455, 489
BciT130I	CCWGG	3 cut(s)	29, 296, 1110
BciVI	GTATCC	1 cut(s)	1270
BclI	TGATCA	2 cut(s)	267, 1231
BcnI	CCSGG	2 cut(s)	667, 668
BcoDI	GTCTC	2 cut(s)	594, 1475
BfaI	CTAG	2 cut(s)	80, 1287
BfmI	CTRYAG	1 cut(s)	720
BfoI	RGCGCY	1 cut(s)	693
BfuAI	ACCTGC	2 cut(s)	812, 1474
BfuI	GTATCC	1 cut(s)	1270
BglI	GCCNNNNNGGC	1 cut(s)	662
BglII	AGATCT	1 cut(s)	905
BisI	GCNGC	8 cut(s)	88, 398, 734, 1443, 1446, 1458, 1461, 1797
BlnI	CCTAGG	1 cut(s)	1286
BlpI	GCTNAGC	1 cut(s)	1449
BlsI	GCNGC	8 cut(s)	89, 399, 735, 1444, 1447, 1459, 1462, 1798
Bme1390I	CCNGG	5 cut(s)	29, 296, 667, 668, 1110
Bme18I	GGWCC	2 cut(s)	253, 747
BmeT110I	CYCGRG	2 cut(s)	376, 666
BmgT120I	GGNCC	8 cut(s)	37, 38, 253, 312, 664, 747, 1304, 1393
BmiI	GGNNCC	5 cut(s)	39, 826, 1093, 1305, 1394
BmrFI	CCNGG	5 cut(s)	29, 296, 667, 668, 1110
BmrI	ACTGGG	1 cut(s)	176
BmsI	GCATC	7 cut(s)	498, 769, 799, 949, 1322, 1783, 1867
BmuI	ACTGGG	1 cut(s)	176
BpiI	GAAGAC	3 cut(s)	153, 512, 1613
BplI	GAGNNNNNCTC	2 cut(s)	271, 303
BpmI	CTGGAG	1 cut(s)	1368
Bpu10I	CCTNAGC	4 cut(s)	737, 893, 1590, 1776
Bpu1102I	GCTNAGC	1 cut(s)	1449
Bpu14I	TTCGAA	1 cut(s)	349
BpuEI	CTTGAG	4 cut(s)	308, 377, 791, 1043
BpuMI	CCSGG	2 cut(s)	667, 668
BsaBI	GATNNNNATC	1 cut(s)	1281
BsaJI	CCNNGG	8 cut(s)	33, 294, 375, 657, 666, 1109, 1286, 1307
BsaXI	ACNNNNNCTCC	6 cut(s)	52, 82, 1137, 1167, 1413, 1443
Bsc4I	CCNNNNNNNGG	7 cut(s)	34, 47, 83, 187, 1391, 1402, 1418
Bse1I	ACTGG	5 cut(s)	182, 358, 514, 691, 1553
Bse21I	CCTNAGG	1 cut(s)	534
Bse8I	GATNNNNATC	1 cut(s)	1281
BseBI	CCWGG	3 cut(s)	29, 296, 1110
BseDI	CCNNGG	8 cut(s)	33, 294, 375, 657, 666, 1109, 1286, 1307
BseGI	GGATG	4 cut(s)	489, 964, 994, 1313
BseJI	GATNNNNATC	1 cut(s)	1281
BseLI	CCNNNNNNNGG	7 cut(s)	34, 47, 83, 187, 1391, 1402, 1418
BseNI	ACTGG	5 cut(s)	182, 358, 514, 691, 1553
BseRI	GAGGAG	3 cut(s)	73, 1018, 1371
BseSI	GKGCMC	2 cut(s)	41, 673
BseXI	GCAGC	6 cut(s)	745, 1429, 1432, 1444, 1447, 1783
BseYI	CCCAGC	1 cut(s)	130
BsgI	GTGCAG	2 cut(s)	621, 1446
Bsh1285I	CGRYCG	1 cut(s)	337
BshNI	GGYRCC	2 cut(s)	824, 1091
BsiEI	CGRYCG	1 cut(s)	337
BsiHKAI	GWGCWC	1 cut(s)	284
BsiHKCI	CYCGRG	2 cut(s)	376, 666
BsiSI	CCGG	2 cut(s)	667, 1396
BslFI	GGGAC	2 cut(s)	535, 925
BslI	CCNNNNNNNGG	7 cut(s)	34, 47, 83, 187, 1391, 1402, 1418
BsmAI	GTCTC	2 cut(s)	594, 1475
BsmFI	GGGAC	2 cut(s)	535, 925
BsmI	GAATGC	2 cut(s)	634, 1081
BsoBI	CYCGRG	2 cut(s)	376, 666
Bsp119I	TTCGAA	1 cut(s)	349
Bsp120I	GGGCCC	1 cut(s)	37
Bsp1286I	GDGCHC	3 cut(s)	41, 284, 673
Bsp143I	GATC	5 cut(s)	267, 529, 905, 1231, 1780
Bsp1720I	GCTNAGC	1 cut(s)	1449
Bsp19I	CCATGG	1 cut(s)	33
BspACI	CCGC	6 cut(s)	88, 337, 389, 397, 447, 782
BspLI	GGNNCC	5 cut(s)	39, 826, 1093, 1305, 1394
BspMAI	CTGCAG	1 cut(s)	724
BspMI	ACCTGC	2 cut(s)	812, 1474
BspPI	GGATC	2 cut(s)	524, 1788
BspT104I	TTCGAA	1 cut(s)	349
BspT107I	GGYRCC	2 cut(s)	824, 1091
BsrBI	CCGCTC	1 cut(s)	391
BsrI	ACTGG	5 cut(s)	182, 358, 514, 691, 1553
BssECI	CCNNGG	8 cut(s)	33, 294, 375, 657, 666, 1109, 1286, 1307
BssMI	GATC	5 cut(s)	267, 529, 905, 1231, 1780
BssSI	CACGAG	1 cut(s)	464
BssT1I	CCWWGG	4 cut(s)	33, 657, 1286, 1307
Bst2BI	CACGAG	1 cut(s)	464
Bst2UI	CCWGG	3 cut(s)	29, 296, 1110
Bst4CI	ACNGT	4 cut(s)	105, 518, 843, 1544
Bst6I	CTCTTC	2 cut(s)	375, 463
BstAPI	GCANNNNNTGC	2 cut(s)	1076, 1442
BstBI	TTCGAA	1 cut(s)	349
BstC8I	GCNNGC	6 cut(s)	14, 447, 628, 632, 764, 1455
BstDSI	CCRYGG	1 cut(s)	33
BstEII	GGTNACC	1 cut(s)	937
BstF5I	GGATG	4 cut(s)	489, 964, 994, 1313
BstH2I	RGCGCY	1 cut(s)	693
BstHHI	GCGC	1 cut(s)	692
BstKTI	GATC	5 cut(s)	270, 532, 908, 1234, 1783
BstMAI	GTCTC	2 cut(s)	594, 1475
BstMBI	GATC	5 cut(s)	267, 529, 905, 1231, 1780
BstMCI	CGRYCG	1 cut(s)	337
BstMWI	GCNNNNNNNGC	9 cut(s)	397, 662, 1076, 1220, 1363, 1442, 1454, 1466, 1796
BstNI	CCWGG	3 cut(s)	29, 296, 1110
BstPI	GGTNACC	1 cut(s)	937
BstSCI	CCNGG	5 cut(s)	27, 294, 665, 666, 1108
BstSFI	CTRYAG	1 cut(s)	720
BstSLI	GKGCMC	2 cut(s)	41, 673
BstV1I	GCAGC	6 cut(s)	745, 1429, 1432, 1444, 1447, 1783
BstV2I	GAAGAC	3 cut(s)	153, 512, 1613
BstX2I	RGATCY	1 cut(s)	905
BstYI	RGATCY	1 cut(s)	905
Bsu36I	CCTNAGG	1 cut(s)	534
BsuI	GTATCC	1 cut(s)	1270
BtgI	CCRYGG	1 cut(s)	33
BtsCI	GGATG	4 cut(s)	489, 964, 994, 1313
BtsI	GCAGTG	2 cut(s)	1077, 1209
BtsIMutI	CAGTG	9 cut(s)	204, 314, 523, 684, 839, 1077, 1209, 1476, 1800
BveI	ACCTGC	2 cut(s)	812, 1474
Cac8I	GCNNGC	6 cut(s)	14, 447, 628, 632, 764, 1455
CaiI	CAGNNNCTG	2 cut(s)	1559, 1880
CfoI	GCGC	1 cut(s)	692
Cfr13I	GGNCC	8 cut(s)	37, 38, 253, 312, 664, 747, 1304, 1393
Cfr9I	CCCGGG	1 cut(s)	666
Csp6I	GTAC	2 cut(s)	382, 825
CviQI	GTAC	2 cut(s)	382, 825
DpnI	GATC	5 cut(s)	269, 531, 907, 1233, 1782
DpnII	GATC	5 cut(s)	267, 529, 905, 1231, 1780
DraIII	CACNNNGTG	1 cut(s)	845
EaeI	YGGCCR	1 cut(s)	85
Eam1104I	CTCTTC	2 cut(s)	375, 463
EarI	CTCTTC	2 cut(s)	375, 463
Ecl136II	GAGCTC	1 cut(s)	282
Eco130I	CCWWGG	4 cut(s)	33, 657, 1286, 1307
Eco24I	GRGCYC	2 cut(s)	41, 284
Eco32I	GATATC	1 cut(s)	717
Eco47I	GGWCC	2 cut(s)	253, 747
Eco53kI	GAGCTC	1 cut(s)	282
Eco57I	CTGAAG	2 cut(s)	147, 1650
Eco81I	CCTNAGG	1 cut(s)	534
Eco88I	CYCGRG	2 cut(s)	376, 666
Eco91I	GGTNACC	1 cut(s)	937
EcoICRI	GAGCTC	1 cut(s)	282
EcoO65I	GGTNACC	1 cut(s)	937
EcoRII	CCWGG	3 cut(s)	27, 294, 1108
EcoRV	GATATC	1 cut(s)	717
EcoT14I	CCWWGG	4 cut(s)	33, 657, 1286, 1307
EcoT22I	ATGCAT	1 cut(s)	948
EcoT38I	GRGCYC	2 cut(s)	41, 284
ErhI	CCWWGG	4 cut(s)	33, 657, 1286, 1307
FaqI	GGGAC	2 cut(s)	535, 925
FauI	CCCGC	2 cut(s)	396, 454
FauNDI	CATATG	1 cut(s)	882
FbaI	TGATCA	2 cut(s)	267, 1231
Fnu4HI	GCNGC	8 cut(s)	88, 398, 734, 1443, 1446, 1458, 1461, 1797
FokI	GGATG	4 cut(s)	476, 971, 1001, 1300
FriOI	GRGCYC	2 cut(s)	41, 284
Fsp4HI	GCNGC	8 cut(s)	88, 398, 734, 1443, 1446, 1458, 1461, 1797
FspBI	CTAG	2 cut(s)	80, 1287
GlaI	GCGC	1 cut(s)	691
GluI	GCNGC	8 cut(s)	88, 398, 734, 1443, 1446, 1458, 1461, 1797
GsaI	CCCAGC	1 cut(s)	134
GsuI	CTGGAG	1 cut(s)	1368
HaeII	RGCGCY	1 cut(s)	693
HapII	CCGG	2 cut(s)	667, 1396
HhaI	GCGC	1 cut(s)	692
Hin6I	GCGC	1 cut(s)	690
HinP1I	GCGC	1 cut(s)	690
HindIII	AAGCTT	1 cut(s)	764
HinfI	GANTC	8 cut(s)	67, 286, 523, 677, 1064, 1105, 1424, 1661
HpaII	CCGG	2 cut(s)	667, 1396
HphI	GGTGA	8 cut(s)	932, 1187, 1276, 1589, 1702, 1716, 1829, 1835
Hpy166II	GTNNAC	2 cut(s)	253, 1724
Hpy188III	TCNNGA	7 cut(s)	325, 437, 527, 727, 770, 805, 1385
Hpy8I	GTNNAC	2 cut(s)	253, 1724
Hpy99I	CGWCG	1 cut(s)	173
HpyAV	CCTTC	6 cut(s)	196, 503, 1334, 1498, 1681, 1807
HpyCH4III	ACNGT	4 cut(s)	105, 518, 843, 1544
HpyF10VI	GCNNNNNNNGC	9 cut(s)	397, 662, 1076, 1220, 1363, 1442, 1454, 1466, 1796
HspAI	GCGC	1 cut(s)	690
KpnI	GGTACC	1 cut(s)	828
Ksp22I	TGATCA	2 cut(s)	267, 1231
Kzo9I	GATC	5 cut(s)	267, 529, 905, 1231, 1780
LmnI	GCTCC	2 cut(s)	132, 889
Lsp1109I	GCAGC	6 cut(s)	745, 1429, 1432, 1444, 1447, 1783
LweI	GCATC	7 cut(s)	498, 769, 799, 949, 1322, 1783, 1867
MaeI	CTAG	2 cut(s)	80, 1287
MaeIII	GTNAC	3 cut(s)	512, 538, 937
MalI	GATC	5 cut(s)	269, 531, 907, 1233, 1782
MbiI	CCGCTC	1 cut(s)	391
MboI	GATC	5 cut(s)	267, 529, 905, 1231, 1780
MflI	RGATCY	1 cut(s)	905
MhlI	GDGCHC	3 cut(s)	41, 284, 673
MluCI	AATT	5 cut(s)	1134, 1194, 1328, 1346, 1399
MlyI	GAGTC	4 cut(s)	76, 280, 517, 1058
Mph1103I	ATGCAT	1 cut(s)	948
MseI	TTAA	2 cut(s)	483, 1254
MslI	CAYNNNNRTG	3 cut(s)	197, 307, 1214
MspA1I	CMGCKG	1 cut(s)	641
MspI	CCGG	2 cut(s)	667, 1396
MspR9I	CCNGG	5 cut(s)	29, 296, 667, 668, 1110
Mva1269I	GAATGC	2 cut(s)	634, 1081
MvaI	CCWGG	3 cut(s)	29, 296, 1110
MwoI	GCNNNNNNNGC	9 cut(s)	397, 662, 1076, 1220, 1363, 1442, 1454, 1466, 1796
NciI	CCSGG	2 cut(s)	667, 668
NcoI	CCATGG	1 cut(s)	33
NdeI	CATATG	1 cut(s)	882
NdeII	GATC	5 cut(s)	267, 529, 905, 1231, 1780
NlaIV	GGNNCC	5 cut(s)	39, 826, 1093, 1305, 1394
NmuCI	GTSAC	1 cut(s)	512
NsiI	ATGCAT	1 cut(s)	948
NspV	TTCGAA	1 cut(s)	349
OliI	CACNNNNGTG	1 cut(s)	1214
PaqCI	CACCTGC	1 cut(s)	1474
PcsI	WCGNNNNNNNCGW	1 cut(s)	493
PctI	GAATGC	2 cut(s)	634, 1081
PfeI	GAWTC	4 cut(s)	677, 1105, 1424, 1661
PflMI	CCANNNNNTGG	1 cut(s)	34
PkrI	GCNGC	8 cut(s)	89, 399, 735, 1444, 1447, 1459, 1462, 1798
PleI	GAGTC	4 cut(s)	75, 280, 517, 1058
PpsI	GAGTC	4 cut(s)	75, 280, 517, 1058
Psp124BI	GAGCTC	1 cut(s)	284
Psp6I	CCWGG	3 cut(s)	27, 294, 1108
PspEI	GGTNACC	1 cut(s)	937
PspFI	CCCAGC	1 cut(s)	130
PspGI	CCWGG	3 cut(s)	27, 294, 1108
PspN4I	GGNNCC	5 cut(s)	39, 826, 1093, 1305, 1394
PspOMI	GGGCCC	1 cut(s)	37
PspPI	GGNCC	8 cut(s)	37, 38, 253, 312, 664, 747, 1304, 1393
PstI	CTGCAG	1 cut(s)	724
PstNI	CAGNNNCTG	2 cut(s)	1559, 1880
PsuI	RGATCY	1 cut(s)	905
PvuII	CAGCTG	1 cut(s)	641
RsaI	GTAC	2 cut(s)	383, 826
RsaNI	GTAC	2 cut(s)	382, 825
RseI	CAYNNNNRTG	3 cut(s)	197, 307, 1214
SacI	GAGCTC	1 cut(s)	284
SaqAI	TTAA	2 cut(s)	483, 1254
SatI	GCNGC	8 cut(s)	88, 398, 734, 1443, 1446, 1458, 1461, 1797
Sau3AI	GATC	5 cut(s)	267, 529, 905, 1231, 1780
Sau96I	GGNCC	8 cut(s)	37, 38, 253, 312, 664, 747, 1304, 1393
SchI	GAGTC	4 cut(s)	76, 280, 517, 1058
ScrFI	CCNGG	5 cut(s)	29, 296, 667, 668, 1110
SduI	GDGCHC	3 cut(s)	41, 284, 673
SfaNI	GCATC	7 cut(s)	498, 769, 799, 949, 1322, 1783, 1867
SfcI	CTRYAG	1 cut(s)	720
SfuI	TTCGAA	1 cut(s)	349
SinI	GGWCC	2 cut(s)	253, 747
SmaI	CCCGGG	1 cut(s)	668
SmiMI	CAYNNNNRTG	3 cut(s)	197, 307, 1214
SmlI	CTYRAG	4 cut(s)	323, 392, 770, 1022
SmoI	CTYRAG	4 cut(s)	323, 392, 770, 1022
SrfI	GCCCGGGC	1 cut(s)	668
Sse9I	AATT	5 cut(s)	1134, 1194, 1328, 1346, 1399
SsiI	CCGC	6 cut(s)	88, 337, 389, 397, 447, 782
SspMI	CTAG	2 cut(s)	80, 1287
SstI	GAGCTC	1 cut(s)	284
StyD4I	CCNGG	5 cut(s)	27, 294, 665, 666, 1108
StyI	CCWWGG	4 cut(s)	33, 657, 1286, 1307
TaaI	ACNGT	4 cut(s)	105, 518, 843, 1544
TaqI	TCGA	2 cut(s)	284, 349
TaqII	GACCGA	1 cut(s)	582
TasI	AATT	5 cut(s)	1134, 1194, 1328, 1346, 1399
TauI	GCSGC	2 cut(s)	90, 400
TfiI	GAWTC	4 cut(s)	677, 1105, 1424, 1661
Tru1I	TTAA	2 cut(s)	483, 1254
Tru9I	TTAA	2 cut(s)	483, 1254
TscAI	CASTG	9 cut(s)	204, 314, 523, 691, 846, 1077, 1216, 1483, 1807
TseFI	GTSAC	1 cut(s)	512
TseI	GCWGC	6 cut(s)	733, 1442, 1445, 1457, 1460, 1796
Tsp45I	GTSAC	1 cut(s)	512
TspDTI	ATGAA	3 cut(s)	771, 1146, 1334
TspMI	CCCGGG	1 cut(s)	666
TspRI	CASTG	9 cut(s)	204, 314, 523, 691, 846, 1077, 1216, 1483, 1807
Van91I	CCANNNNNTGG	1 cut(s)	34
VpaK11BI	GGWCC	2 cut(s)	253, 747
XapI	RAATTY	1 cut(s)	1399
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1216
XmaI	CCCGGG	1 cut(s)	666
XmaJI	CCTAGG	1 cut(s)	1286
XspI	CTAG	2 cut(s)	80, 1287
Zsp2I	ATGCAT	1 cut(s)	948

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.