Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0010475

Physical Location & Seq

Genomic Coordinates Reverse (-)

34388409 .. 34390498

Gene Structure 2090 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1267900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1209 bp

ATGATCAAGCCCCAGAGCTCAACTCCCTCCTTGGACACCTCTCAGTGGCCTATCCTTCTCAAAAACTATGACCGACTCAATGTTCGAACTGGACACTACACCCCTCTACCCTCAGGGTTCTCCCCCCTTAAGAGGCCTCTTGCTGAGTACATCCGATATGGTGTTCTCAATCTTGACAAGCCTGCAAACCCCTCTTCTCACGAGGTTGTTGCTTGGATTAAGCGCATCCTTCGCGTTGAGAAAACTGGCCACAGCGGTACTCTGGATCCTAAGGTTACAGGTAATCTCATTGTCTGCATTGACCGAGCCACACGACTTGTCAAGTCCCAGCAGGGTGCAGGGAAAGAGTATGTTTGTGTTGCCCGCTTGCATTCAGCTGTGCCTGATGTTGCCAAAGTGGCTCAGGCCCTTGAATCTCTTACTGGGGCTGTCTTTCAGAGGCCACCTTTGATATCAGCAGTCAAGAGGCAGCTTAGGATTAGGACCATCTATGAAAGCAAACTTCTTGAGTTTGATGCAGATAAACATTTGGTCGTTTTCTGGATTTCTTGCGAAGCAGGAACCTATGTGCGGACGCTCTGTGTGCATTTGGGTCTGCTGCTTGGTGTGGGAGGTCATATGCAGGAGCTTAGGAGGGTCAGGTCGGGGATTATGGGCGAGAAAGACAACATGGTCACAATGCATGACGTCATGGATGCTCAGTGGGCTTATGACACTAATAGGGATGAGAGTTATTTGAGGAGGGTCATCATGCCTCTTGAGGTTCTCTTGACCAGTTATAAGAGATTGGTTGTCAAGGACTCAGCAGTGAATGCCATTTGCTACGGTGCCAAGTTAATGATTCCAGGGCTGCTCAGGTTTGAGAATGATATCGAGGTTGGAGAGGAAGTTGTGCTGATGACTACCAAAGGAGAAGCCATTGCATTGGGAATTGCTGAGATGACCACTGCTGTGATGGCAACTTGTGATCATGGTGTGGTGGCAAAGATTAAGAGGGTGGTAATGGATCGGGATACTTACCCAAGGAAGTGGGGATTGGGACCAAGGGCGTCCATGAAGAAGAAATTGATTGCCGAAGGGAAATTGGATAAGCATGGGAAGCCCACTGAGAATACTCCGCAAGAATGGATGAGAAATGTGGTTTTGTCTGCAGGGAGGAGACCCGCATTTTCTTTTTTTTTTGCAAGACTATCAATTCCTTTCAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000154 GO:0000495 GO:0001522 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005732 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009506 GO:0009982 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0016072 GO:0016073 GO:0016074 GO:0016556 GO:0016853 GO:0016866 GO:0022613 GO:0030054 GO:0031118 GO:0031120 GO:0031123 GO:0031126 GO:0031429 GO:0031974 GO:0031981 GO:0032991 GO:0033979 GO:0034470 GO:0034641 GO:0034660 GO:0034964 GO:0040031 GO:0042254 GO:0043144 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043628 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0055044 GO:0070013 GO:0071704 GO:0071840 GO:0072588 GO:0090304 GO:1901360 GO:1902494 GO:1990481 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03008 map03008

Pfam Domains

Protein Families

DKCLD PUA TruB_C_2 TruB_N

Protein Analysis

403

Amino Acids

45.02

Weight (kDa)

9.6

Isoelectric Point (pI)

32.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DKCLD	PF08068	13 - 70	2.1e-31	DKCLD (NUC011) domain
TruB_N	PF01509	74 - 190	3.2e-21	TruB family pseudouridylate synthase (N terminal domain)
TruB_C_2	PF16198	191 - 257	5.9e-27	tRNA pseudouridylate synthase B C-terminal domain
PUA	PF01472	261 - 334	5.9e-24	PUA domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0010475)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G57150
fragaria_vesca	FvH4_5g20900 FvH4_5g20900 FvH4_5g20900
malus_domestica	MD06G1043100.v1.1 MD16G1267700.v1.1 MD16G1267900.v1.1
prunus_persica	Prupe.1G572500_v2.0.a1 Prupe.5G050000_v2.0.a1
pyrus_communis	pycom06g03610 pycom16g23720
rosa_chinensis	RchiOBHm_Chr7g0207911
rosa_laevigata	RLG00000003276
rosa_multiflora	Rmu_sc0006577.1_g000004
rosa_roxburghii	Rroxscaffold_3G00251050
rosa_rugosa	Rorug07G0100200
rosa_samantha	Rh7AG233600 Rh7BG229200 Rh7CG248900 Rh7DG239600
rosa_wichuraiana	Rw0G001290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	780
AasI	GACNNNNNNGTC	1 cut(s)	671
AatII	GACGTC	1 cut(s)	690
AccB1I	GGYRCC	1 cut(s)	827
AccII	CGCG	1 cut(s)	234
AciI	CCGC	5 cut(s)	255, 364, 571, 1118, 1164
AclWI	GGATC	3 cut(s)	260, 273, 1014
AcoI	YGGCCR	1 cut(s)	247
AcyI	GRCGYC	2 cut(s)	687, 1049
AfaI	GTAC	2 cut(s)	149, 259
AfiI	CCNNNNNNNGG	3 cut(s)	45, 132, 570
AflII	CTTAAG	1 cut(s)	128
AgsI	TTSAA	2 cut(s)	413, 1204
AjnI	CCWGG	1 cut(s)	844
AjuI	GAANNNNNNNTTGG	2 cut(s)	1019, 1051
AleI	CACNNNNGTG	1 cut(s)	950
AluBI	AGCT	4 cut(s)	18, 377, 472, 628
AluI	AGCT	4 cut(s)	18, 377, 472, 628
Alw21I	GWGCWC	1 cut(s)	20
Alw26I	GTCTC	1 cut(s)	1153
AlwI	GGATC	3 cut(s)	260, 273, 1014
AoxI	GGCC	5 cut(s)	47, 134, 247, 405, 440
ApeKI	GCWGC	3 cut(s)	469, 598, 850
ArsI	GACNNNNNNTTYG	2 cut(s)	66, 98
AspLEI	GCGC	1 cut(s)	225
AspS9I	GGNCC	3 cut(s)	406, 483, 1040
AsuII	TTCGAA	1 cut(s)	85
AvaII	GGWCC	2 cut(s)	483, 1040
AxyI	CCTNAGG	2 cut(s)	112, 270
BalI	TGGCCA	1 cut(s)	249
BamHI	GGATCC	1 cut(s)	265
BanI	GGYRCC	1 cut(s)	827
BanII	GRGCYC	1 cut(s)	20
BauI	CACGAG	1 cut(s)	200
Bbv12I	GWGCWC	1 cut(s)	20
BbvI	GCAGC	3 cut(s)	481, 585, 837
BccI	CCATC	2 cut(s)	494, 949
BcgI	CGANNNNNNTGC	2 cut(s)	191, 225
BciT130I	CCWGG	1 cut(s)	846
BciVI	GTATCC	1 cut(s)	1006
BclI	TGATCA	2 cut(s)	3, 967
BcoDI	GTCTC	1 cut(s)	1153
BfmI	CTRYAG	1 cut(s)	1149
BfrI	CTTAAG	1 cut(s)	128
BfuI	GTATCC	1 cut(s)	1006
BglI	GCCNNNNNGGC	1 cut(s)	398
BisI	GCNGC	3 cut(s)	470, 599, 851
BlsI	GCNGC	3 cut(s)	471, 600, 852
Bme1390I	CCNGG	1 cut(s)	846
Bme18I	GGWCC	2 cut(s)	483, 1040
BmgT120I	GGNCC	3 cut(s)	406, 483, 1040
BmiI	GGNNCC	4 cut(s)	267, 562, 829, 1041
BmrFI	CCNGG	1 cut(s)	846
BmrI	ACTGGG	1 cut(s)	432
BmsI	GCATC	3 cut(s)	234, 505, 685
BmuI	ACTGGG	1 cut(s)	432
BplI	GAGNNNNNCTC	2 cut(s)	7, 39
Bpu10I	CCTNAGC	4 cut(s)	402, 473, 629, 854
Bpu14I	TTCGAA	1 cut(s)	85
BpuEI	CTTGAG	2 cut(s)	527, 779
BsaHI	GRCGYC	2 cut(s)	687, 1049
BsaI	GGTCTC	1 cut(s)	1153
BsaJI	CCNNGG	4 cut(s)	30, 845, 1022, 1043
BsaXI	ACNNNNNCTCC	2 cut(s)	873, 903
Bsc4I	CCNNNNNNNGG	3 cut(s)	45, 132, 570
Bse1I	ACTGG	4 cut(s)	94, 250, 427, 774
Bse21I	CCTNAGG	2 cut(s)	112, 270
Bse3DI	GCAATG	1 cut(s)	918
BseBI	CCWGG	1 cut(s)	846
BseDI	CCNNGG	4 cut(s)	30, 845, 1022, 1043
BseGI	GGATG	5 cut(s)	150, 225, 700, 730, 1134
BseLI	CCNNNNNNNGG	3 cut(s)	45, 132, 570
BseMI	GCAATG	1 cut(s)	918
BseMII	CTCAG	9 cut(s)	56, 126, 135, 416, 713, 816, 868, 927, 1098
BseNI	ACTGG	4 cut(s)	94, 250, 427, 774
BseRI	GAGGAG	2 cut(s)	754, 1171
BseXI	GCAGC	3 cut(s)	481, 585, 837
BseYI	CCCAGC	1 cut(s)	327
BsgI	GTGCAG	1 cut(s)	357
Bsh1236I	CGCG	1 cut(s)	234
BshFI	GGCC	5 cut(s)	49, 136, 249, 407, 442
BshNI	GGYRCC	1 cut(s)	827
BsiHKAI	GWGCWC	1 cut(s)	20
BslFI	GGGAC	2 cut(s)	310, 1053
BslI	CCNNNNNNNGG	3 cut(s)	45, 132, 570
BsmAI	GTCTC	1 cut(s)	1153
BsmFI	GGGAC	2 cut(s)	310, 1053
BsmI	GAATGC	2 cut(s)	370, 817
BsnI	GGCC	5 cut(s)	49, 136, 249, 407, 442
Bso31I	GGTCTC	1 cut(s)	1153
Bsp119I	TTCGAA	1 cut(s)	85
Bsp1286I	GDGCHC	1 cut(s)	20
Bsp143I	GATC	4 cut(s)	3, 265, 967, 1006
BspACI	CCGC	5 cut(s)	255, 364, 571, 1118, 1164
BspANI	GGCC	5 cut(s)	49, 136, 249, 407, 442
BspCNI	CTCAG	9 cut(s)	55, 125, 136, 415, 712, 815, 867, 928, 1099
BspFNI	CGCG	1 cut(s)	234
BspLI	GGNNCC	4 cut(s)	267, 562, 829, 1041
BspMAI	CTGCAG	1 cut(s)	1153
BspPI	GGATC	3 cut(s)	260, 273, 1014
BspT104I	TTCGAA	1 cut(s)	85
BspT107I	GGYRCC	1 cut(s)	827
BspTI	CTTAAG	1 cut(s)	128
BspTNI	GGTCTC	1 cut(s)	1153
BsrDI	GCAATG	1 cut(s)	918
BsrI	ACTGG	4 cut(s)	94, 250, 427, 774
BssECI	CCNNGG	4 cut(s)	30, 845, 1022, 1043
BssMI	GATC	4 cut(s)	3, 265, 967, 1006
BssNI	GRCGYC	2 cut(s)	687, 1049
BssSI	CACGAG	1 cut(s)	200
BssT1I	CCWWGG	3 cut(s)	30, 1022, 1043
Bst2BI	CACGAG	1 cut(s)	200
Bst2UI	CCWGG	1 cut(s)	846
Bst4CI	ACNGT	1 cut(s)	827
Bst6I	CTCTTC	1 cut(s)	199
BstACI	GRCGYC	2 cut(s)	687, 1049
BstAFI	CTTAAG	1 cut(s)	128
BstAPI	GCANNNNNTGC	1 cut(s)	812
BstBI	TTCGAA	1 cut(s)	85
BstC8I	GCNNGC	3 cut(s)	183, 364, 368
BstF5I	GGATG	5 cut(s)	150, 225, 700, 730, 1134
BstFNI	CGCG	1 cut(s)	234
BstHHI	GCGC	1 cut(s)	225
BstKTI	GATC	4 cut(s)	6, 268, 970, 1009
BstMAI	GTCTC	1 cut(s)	1153
BstMBI	GATC	4 cut(s)	3, 265, 967, 1006
BstMWI	GCNNNNNNNGC	7 cut(s)	231, 398, 583, 704, 812, 956, 1099
BstNI	CCWGG	1 cut(s)	846
BstSCI	CCNGG	1 cut(s)	844
BstSFI	CTRYAG	1 cut(s)	1149
BstUI	CGCG	1 cut(s)	234
BstV1I	GCAGC	3 cut(s)	481, 585, 837
BstX2I	RGATCY	1 cut(s)	265
BstXI	CCANNNNNNTGG	2 cut(s)	925, 1029
BstYI	RGATCY	1 cut(s)	265
Bsu36I	CCTNAGG	2 cut(s)	112, 270
BsuI	GTATCC	1 cut(s)	1006
BsuRI	GGCC	5 cut(s)	49, 136, 249, 407, 442
BtsCI	GGATG	5 cut(s)	150, 225, 700, 730, 1134
BtsI	GCAGTG	2 cut(s)	813, 945
BtsIMutI	CAGTG	5 cut(s)	50, 707, 813, 945, 1104
Cac8I	GCNNGC	3 cut(s)	183, 364, 368
CfoI	GCGC	1 cut(s)	225
Cfr13I	GGNCC	3 cut(s)	406, 483, 1040
CseI	GACGC	2 cut(s)	583, 1038
Csp6I	GTAC	2 cut(s)	148, 258
CspCI	CAANNNNNGTGG	2 cut(s)	298, 333
CviAII	CATG	7 cut(s)	670, 683, 691, 751, 971, 1054, 1094
CviQI	GTAC	2 cut(s)	148, 258
DpnI	GATC	4 cut(s)	5, 267, 969, 1008
DpnII	GATC	4 cut(s)	3, 265, 967, 1006
DrdI	GACNNNNNNGTC	1 cut(s)	671
DseDI	GACNNNNNNGTC	1 cut(s)	671
EaeI	YGGCCR	1 cut(s)	247
Eam1104I	CTCTTC	1 cut(s)	199
EarI	CTCTTC	1 cut(s)	199
Ecl136II	GAGCTC	1 cut(s)	18
Eco130I	CCWWGG	3 cut(s)	30, 1022, 1043
Eco147I	AGGCCT	1 cut(s)	136
Eco24I	GRGCYC	1 cut(s)	20
Eco31I	GGTCTC	1 cut(s)	1153
Eco32I	GATATC	2 cut(s)	453, 871
Eco47I	GGWCC	2 cut(s)	483, 1040
Eco53kI	GAGCTC	1 cut(s)	18
Eco81I	CCTNAGG	2 cut(s)	112, 270
EcoICRI	GAGCTC	1 cut(s)	18
EcoO109I	RGGNCCY	1 cut(s)	406
EcoRII	CCWGG	1 cut(s)	844
EcoRV	GATATC	2 cut(s)	453, 871
EcoT14I	CCWWGG	3 cut(s)	30, 1022, 1043
EcoT22I	ATGCAT	1 cut(s)	684
EcoT38I	GRGCYC	1 cut(s)	20
ErhI	CCWWGG	3 cut(s)	30, 1022, 1043
FaeI	CATG	7 cut(s)	673, 686, 694, 754, 974, 1057, 1097
FaqI	GGGAC	2 cut(s)	310, 1053
FatI	CATG	7 cut(s)	669, 682, 690, 750, 970, 1053, 1093
FauI	CCCGC	2 cut(s)	371, 1171
FauNDI	CATATG	1 cut(s)	618
FbaI	TGATCA	2 cut(s)	3, 967
Fnu4HI	GCNGC	3 cut(s)	470, 599, 851
FokI	GGATG	5 cut(s)	137, 212, 707, 737, 1141
FriOI	GRGCYC	1 cut(s)	20
Fsp4HI	GCNGC	3 cut(s)	470, 599, 851
GlaI	GCGC	1 cut(s)	224
GluI	GCNGC	3 cut(s)	470, 599, 851
GsaI	CCCAGC	1 cut(s)	331
HaeIII	GGCC	5 cut(s)	49, 136, 249, 407, 442
HgaI	GACGC	2 cut(s)	583, 1038
HhaI	GCGC	1 cut(s)	225
Hin1I	GRCGYC	2 cut(s)	687, 1049
Hin1II	CATG	7 cut(s)	673, 686, 694, 754, 974, 1057, 1097
Hin6I	GCGC	1 cut(s)	223
HinP1I	GCGC	1 cut(s)	223
HinfI	GANTC	4 cut(s)	75, 413, 800, 841
Hpy188I	TCNGA	2 cut(s)	155, 438
Hpy188III	TCNNGA	9 cut(s)	173, 200, 263, 463, 506, 541, 758, 769, 1010
HpyAV	CCTTC	3 cut(s)	65, 239, 1070
HpyCH4III	ACNGT	1 cut(s)	827
HpyCH4IV	ACGT	1 cut(s)	687
HpyF10VI	GCNNNNNNNGC	7 cut(s)	231, 398, 583, 704, 812, 956, 1099
HpySE526I	ACGT	1 cut(s)	687
Hsp92I	GRCGYC	2 cut(s)	687, 1049
Hsp92II	CATG	7 cut(s)	673, 686, 694, 754, 974, 1057, 1097
HspAI	GCGC	1 cut(s)	223
Ksp22I	TGATCA	2 cut(s)	3, 967
Kzo9I	GATC	4 cut(s)	3, 265, 967, 1006
LmnI	GCTCC	1 cut(s)	625
Lsp1109I	GCAGC	3 cut(s)	481, 585, 837
LweI	GCATC	3 cut(s)	234, 505, 685
MaeII	ACGT	1 cut(s)	687
MaeIII	GTNAC	2 cut(s)	274, 673
MalI	GATC	4 cut(s)	5, 267, 969, 1008
MboI	GATC	4 cut(s)	3, 265, 967, 1006
MboII	GAAGA	3 cut(s)	186, 1069, 1072
MfeI	CAATTG	1 cut(s)	1204
MflI	RGATCY	1 cut(s)	265
MhlI	GDGCHC	1 cut(s)	20
MlsI	TGGCCA	1 cut(s)	249
MluCI	AATT	5 cut(s)	930, 1064, 1082, 1194, 1204
MluNI	TGGCCA	1 cut(s)	249
MlyI	GAGTC	2 cut(s)	69, 794
MmeI	TCCRAC	1 cut(s)	859
Mox20I	TGGCCA	1 cut(s)	249
Mph1103I	ATGCAT	1 cut(s)	684
MscI	TGGCCA	1 cut(s)	249
MseI	TTAA	4 cut(s)	129, 219, 836, 990
MslI	CAYNNNNRTG	1 cut(s)	950
Msp20I	TGGCCA	1 cut(s)	249
MspA1I	CMGCKG	2 cut(s)	255, 377
MspCI	CTTAAG	1 cut(s)	128
MspR9I	CCNGG	1 cut(s)	846
MunI	CAATTG	1 cut(s)	1204
Mva1269I	GAATGC	2 cut(s)	370, 817
MvaI	CCWGG	1 cut(s)	846
MvnI	CGCG	1 cut(s)	234
MwoI	GCNNNNNNNGC	7 cut(s)	231, 398, 583, 704, 812, 956, 1099
NdeI	CATATG	1 cut(s)	618
NdeII	GATC	4 cut(s)	3, 265, 967, 1006
NlaIII	CATG	7 cut(s)	673, 686, 694, 754, 974, 1057, 1097
NlaIV	GGNNCC	4 cut(s)	267, 562, 829, 1041
NmuCI	GTSAC	1 cut(s)	673
NsiI	ATGCAT	1 cut(s)	684
NspV	TTCGAA	1 cut(s)	85
OliI	CACNNNNGTG	1 cut(s)	950
PceI	AGGCCT	1 cut(s)	136
PctI	GAATGC	2 cut(s)	370, 817
PfeI	GAWTC	2 cut(s)	413, 841
PkrI	GCNGC	3 cut(s)	471, 600, 852
PleI	GAGTC	2 cut(s)	69, 794
PpsI	GAGTC	2 cut(s)	69, 794
PsiI	TTATAA	1 cut(s)	780
Psp124BI	GAGCTC	1 cut(s)	20
Psp6I	CCWGG	1 cut(s)	844
PspFI	CCCAGC	1 cut(s)	327
PspGI	CCWGG	1 cut(s)	844
PspN4I	GGNNCC	4 cut(s)	267, 562, 829, 1041
PspPI	GGNCC	3 cut(s)	406, 483, 1040
PstI	CTGCAG	1 cut(s)	1153
PsuI	RGATCY	1 cut(s)	265
PvuII	CAGCTG	1 cut(s)	377
RsaI	GTAC	2 cut(s)	149, 259
RsaNI	GTAC	2 cut(s)	148, 258
RseI	CAYNNNNRTG	1 cut(s)	950
SacI	GAGCTC	1 cut(s)	20
SaqAI	TTAA	4 cut(s)	129, 219, 836, 990
SatI	GCNGC	3 cut(s)	470, 599, 851
Sau3AI	GATC	4 cut(s)	3, 265, 967, 1006
Sau96I	GGNCC	3 cut(s)	406, 483, 1040
SchI	GAGTC	2 cut(s)	69, 794
ScrFI	CCNGG	1 cut(s)	846
SduI	GDGCHC	1 cut(s)	20
SfaNI	GCATC	3 cut(s)	234, 505, 685
SfcI	CTRYAG	1 cut(s)	1149
SfuI	TTCGAA	1 cut(s)	85
SinI	GGWCC	2 cut(s)	483, 1040
SmiMI	CAYNNNNRTG	1 cut(s)	950
SmlI	CTYRAG	3 cut(s)	128, 506, 758
SmoI	CTYRAG	3 cut(s)	128, 506, 758
Sse9I	AATT	5 cut(s)	930, 1064, 1082, 1194, 1204
SseBI	AGGCCT	1 cut(s)	136
SsiI	CCGC	5 cut(s)	255, 364, 571, 1118, 1164
SstI	GAGCTC	1 cut(s)	20
StuI	AGGCCT	1 cut(s)	136
StyD4I	CCNGG	1 cut(s)	844
StyI	CCWWGG	3 cut(s)	30, 1022, 1043
TaaI	ACNGT	1 cut(s)	827
TaiI	ACGT	1 cut(s)	690
TaqI	TCGA	2 cut(s)	85, 873
TaqII	GACCGA	2 cut(s)	87, 318
TasI	AATT	5 cut(s)	930, 1064, 1082, 1194, 1204
TatI	WGTACW	1 cut(s)	147
TfiI	GAWTC	2 cut(s)	413, 841
Tru1I	TTAA	4 cut(s)	129, 219, 836, 990
Tru9I	TTAA	4 cut(s)	129, 219, 836, 990
TscAI	CASTG	5 cut(s)	50, 707, 813, 952, 1111
TseFI	GTSAC	1 cut(s)	673
TseI	GCWGC	3 cut(s)	469, 598, 850
Tsp45I	GTSAC	1 cut(s)	673
TspDTI	ATGAA	2 cut(s)	507, 1070
TspRI	CASTG	5 cut(s)	50, 707, 813, 952, 1111
Vha464I	CTTAAG	1 cut(s)	128
VpaK11BI	GGWCC	2 cut(s)	483, 1040
XcmI	CCANNNNNNNNNTGG	1 cut(s)	952
ZraI	GACGTC	1 cut(s)	688
Zsp2I	ATGCAT	1 cut(s)	684

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.