Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0010475

Physical Location & Seq

Genomic Coordinates Forward (+)

5656241 .. 5658896

Gene Structure 2656 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1043100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1620 bp

ATGATCAAGCCCCAGAGCTCAACTCCCTCCTTGGACACCTCTCAGTGGCCTATCCTTCTTAAAAATTATGACCGCCTCAATGTTCGAACGGGACACTACACTCCTCTACCCTCAGGGTTTTCCCCCCTTAAGAGGCCTCTTGCTGAGTACATCCGATATGGTGTTCTCAATCTTGACAAGCCTGCAAACCCCTCGTCGCACGAGGTTGTCGCTTGGATTAAGCGCATCCTTCGTGTTGAGAAAACTGGCCACAGCGGTACCCTGGATCCTAAGGTTACAGGTAATCTCATTGTCTGCATTGACCGAGCCACACGACTTGTCAAGTCCCAGCAGGGTGCAGGGAAAGAGTATGTTTGTGTTGCCCGCTTGCATTCAGCTGTGCCTGATGTTGCCAAGGTGGCTCGGGCCCTTGAATCCCTGACTGGGGCTGTCTTTCAGAGGCCACCTTTGATATCAGCCGTCAAAAGGCAGCTTAGGATTAGGACTATCTATGAAAGCAAGCTTCTTGAGTATGAGGCAGATAAACATTTGGTTGTTTTCTGGATTTCTTGCGAAGCAGGAACCTATGTGCGTACGCTCTGTGTGCATTTGGGTCTGCTTCTTGGTGTGGGAGGTCATATGCAGGAGCTTAGGAGGGTCAGGTCTGGGATCATGGGTGAGAAAGACAACATGGTCACAATGCACGATGTCATGGATGCTCAGTGGGCTTATGACACTAATAGGGATGAGAGTTATTTGAGGAGGGTCATCATGCCTCTTGAGGTTCTCTTGACTAGTTATAAGAGATTGGTTGTCAAGGACTCAGCAGTGAATGCCATTTGCTACGGTGCCAAGTTAATGATTCCAGGGCTGCTCAGGTTTGAGAACGATATCGAGGTAGGAGAGGAAGTTGTGCTGATGACTACCAAAGGAGAAGCCATTGCATTGGGAATTGCTGAGATGACCACTGCTGTGATGGCAACTTGTGATCATGGTGTGGTGGCAAAGATTAAGAGGGTGGTGATGGATCGGGATACTTACCCAAGAAAGTGGGGATTGGGACCGAGGGCATCCATGAAGAAGAAATTGATTGCCGAAGGAAAATTGGATAAGCATGGGAAGCCCACTGAGAATACTCCGCAAGAATGGATGAGGAATGTGGTTTTGCCTGTTGGTGGGGATTCTATTGTTGCAAGCACTGCTGGTGCACTTGCCACTGAAACTGCTGTGGCAGAGAAGGAGACCACTCTCGTAGACAAGGAGAAGAAGGATAAGAAAAAGAAGGACAAACATAAGGATGAAAAGGAAACTAAGGAGGGGCGTAAGCGCAAACTAGAGGAAGCCACAGAGAGCCCTGTTGCAGTTCCTGCGAAGAAAAGCAAAGGCGAAGAAGTTGAGCCTGAAGTTGAGAAGTCAGAAAAGAAAAAGAAGAAAAAGGACAAAGAGAATGGCACCGTGGATAATGTTGAAGCTGGTTCACCTGAAGCAGAAAAGTCTGAGAAGAAGAAGAAGAAGAAGAAAAACAAAGACGCTGAGGACAATGGTACTGTGGCTTCTGCCGGCGCTGGTGATGGCGAGGCTGATAAGAGTGAGAAAAAGAAGAAAAAGAAAAAGAGTAAAGCAGATGCAGAAGAAGTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000154 GO:0000495 GO:0001522 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005732 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009506 GO:0009982 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0016072 GO:0016073 GO:0016074 GO:0016556 GO:0016853 GO:0016866 GO:0022613 GO:0030054 GO:0031118 GO:0031120 GO:0031123 GO:0031126 GO:0031429 GO:0031974 GO:0031981 GO:0032991 GO:0033979 GO:0034470 GO:0034641 GO:0034660 GO:0034964 GO:0040031 GO:0042254 GO:0043144 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043628 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0055044 GO:0070013 GO:0071704 GO:0071840 GO:0072588 GO:0090304 GO:1901360 GO:1902494 GO:1990481 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03008 map03008

Pfam Domains

Protein Families

DKCLD PUA TruB_C_2 TruB_N

Protein Analysis

540

Amino Acids

59.78

Weight (kDa)

9.4

Isoelectric Point (pI)

31.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DKCLD	PF08068	13 - 70	3.2e-31	DKCLD (NUC011) domain
TruB_N	PF01509	74 - 190	9.2e-21	TruB family pseudouridylate synthase (N terminal domain)
TruB_C_2	PF16198	191 - 257	9e-27	tRNA pseudouridylate synthase B C-terminal domain
PUA	PF01472	261 - 334	9.2e-24	PUA domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010475)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G57150
fragaria_vesca	FvH4_5g20900 FvH4_5g20900 FvH4_5g20900
malus_domestica	MD06G1043100.v1.1 MD16G1267700.v1.1 MD16G1267900.v1.1
prunus_persica	Prupe.1G572500_v2.0.a1 Prupe.5G050000_v2.0.a1
pyrus_communis	pycom06g03610 pycom16g23720
rosa_chinensis	RchiOBHm_Chr7g0207911
rosa_laevigata	RLG00000003276
rosa_multiflora	Rmu_sc0006577.1_g000004
rosa_roxburghii	Rroxscaffold_3G00251050
rosa_rugosa	Rorug07G0100200
rosa_samantha	Rh7AG233600 Rh7BG229200 Rh7CG248900 Rh7DG239600
rosa_wichuraiana	Rw0G001290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	780
AasI	GACNNNNNNGTC	1 cut(s)	671
Acc65I	GGTACC	1 cut(s)	257
AccB1I	GGYRCC	3 cut(s)	257, 827, 1430
AccI	GTMKAC	1 cut(s)	1233
AciI	CCGC	4 cut(s)	73, 255, 364, 1118
AclWI	GGATC	4 cut(s)	260, 273, 656, 1014
AcoI	YGGCCR	1 cut(s)	247
AcuI	CTGAAG	2 cut(s)	1401, 1482
AfaI	GTAC	4 cut(s)	149, 259, 574, 1525
AfiI	CCNNNNNNNGG	6 cut(s)	45, 132, 423, 424, 465, 1154
AflII	CTTAAG	1 cut(s)	128
AgsI	TTSAA	2 cut(s)	413, 1448
AhlI	ACTAGT	1 cut(s)	773
AjnI	CCWGG	2 cut(s)	261, 844
AleI	CACNNNNGTG	1 cut(s)	950
AluBI	AGCT	6 cut(s)	18, 377, 472, 502, 628, 1451
AluI	AGCT	6 cut(s)	18, 377, 472, 502, 628, 1451
Alw21I	GWGCWC	2 cut(s)	20, 1189
Alw26I	GTCTC	1 cut(s)	1214
Alw44I	GTGCAC	1 cut(s)	1185
AlwI	GGATC	4 cut(s)	260, 273, 656, 1014
AlwNI	CAGNNNCTG	1 cut(s)	1346
Ama87I	CYCGRG	1 cut(s)	402
AoxI	GGCC	5 cut(s)	47, 134, 247, 405, 440
ApaI	GGGCCC	1 cut(s)	409
ApaLI	GTGCAC	1 cut(s)	1185
ApeKI	GCWGC	2 cut(s)	469, 850
ArsI	GACNNNNNNTTYG	2 cut(s)	179, 211
Asp718I	GGTACC	1 cut(s)	257
AspLEI	GCGC	3 cut(s)	225, 1308, 1544
AspS9I	GGNCC	3 cut(s)	405, 406, 1040
AsuHPI	GGTGA	4 cut(s)	668, 1012, 1449, 1559
AsuII	TTCGAA	1 cut(s)	85
AvaI	CYCGRG	1 cut(s)	402
AvaII	GGWCC	1 cut(s)	1040
AxyI	CCTNAGG	2 cut(s)	112, 270
BaeGI	GKGCMC	2 cut(s)	409, 1189
BaeI	ACNNNNGTAYC	2 cut(s)	241, 274
BalI	TGGCCA	1 cut(s)	249
BamHI	GGATCC	1 cut(s)	265
BanI	GGYRCC	3 cut(s)	257, 827, 1430
BanII	GRGCYC	3 cut(s)	20, 409, 1334
BarI	GAAGNNNNNNTAC	2 cut(s)	1516, 1548
BauI	CACGAG	1 cut(s)	200
Bbv12I	GWGCWC	2 cut(s)	20, 1189
BbvCI	CCTCAGC	1 cut(s)	1512
BbvI	GCAGC	2 cut(s)	481, 837
BccI	CCATC	3 cut(s)	949, 997, 1544
BceAI	ACGGC	1 cut(s)	443
BcgI	CGANNNNNNTGC	2 cut(s)	174, 208
BciT130I	CCWGG	2 cut(s)	263, 846
BciVI	GTATCC	1 cut(s)	1006
BclI	TGATCA	2 cut(s)	3, 967
BcoDI	GTCTC	1 cut(s)	1214
BcuI	ACTAGT	1 cut(s)	773
BfaI	CTAG	2 cut(s)	774, 1313
BfoI	RGCGCY	1 cut(s)	1545
BfrI	CTTAAG	1 cut(s)	128
BfuI	GTATCC	1 cut(s)	1006
BglI	GCCNNNNNGGC	1 cut(s)	398
BisI	GCNGC	2 cut(s)	470, 851
BlsI	GCNGC	2 cut(s)	471, 852
Bme1390I	CCNGG	2 cut(s)	263, 846
Bme18I	GGWCC	1 cut(s)	1040
BmeT110I	CYCGRG	1 cut(s)	402
BmgT120I	GGNCC	3 cut(s)	405, 406, 1040
BmiI	GGNNCC	7 cut(s)	259, 267, 407, 562, 829, 1041, 1432
BmrFI	CCNGG	2 cut(s)	263, 846
BmrI	ACTGGG	1 cut(s)	432
BmsI	GCATC	4 cut(s)	234, 685, 1058, 1594
BmuI	ACTGGG	1 cut(s)	432
BplI	GAGNNNNNCTC	2 cut(s)	7, 39
Bpu10I	CCTNAGC	4 cut(s)	473, 629, 854, 1512
Bpu14I	TTCGAA	1 cut(s)	85
BpuEI	CTTGAG	2 cut(s)	527, 779
BsaI	GGTCTC	1 cut(s)	1214
BsaJI	CCNNGG	6 cut(s)	30, 261, 393, 845, 1043, 1434
BsaXI	ACNNNNNCTCC	2 cut(s)	873, 903
Bsc4I	CCNNNNNNNGG	6 cut(s)	45, 132, 423, 424, 465, 1154
Bse118I	RCCGGY	1 cut(s)	1538
Bse1I	ACTGG	2 cut(s)	250, 427
Bse21I	CCTNAGG	2 cut(s)	112, 270
Bse3DI	GCAATG	1 cut(s)	918
BseBI	CCWGG	2 cut(s)	263, 846
BseDI	CCNNGG	6 cut(s)	30, 261, 393, 845, 1043, 1434
BseGI	GGATG	7 cut(s)	150, 225, 700, 730, 1049, 1134, 1282
BseLI	CCNNNNNNNGG	6 cut(s)	45, 132, 423, 424, 465, 1154
BseMI	GCAATG	1 cut(s)	918
BseNI	ACTGG	2 cut(s)	250, 427
BseRI	GAGGAG	2 cut(s)	93, 754
BseSI	GKGCMC	2 cut(s)	409, 1189
BseXI	GCAGC	2 cut(s)	481, 837
BseYI	CCCAGC	1 cut(s)	327
BsgI	GTGCAG	1 cut(s)	357
BshFI	GGCC	5 cut(s)	49, 136, 249, 407, 442
BshNI	GGYRCC	3 cut(s)	257, 827, 1430
BsiHKAI	GWGCWC	2 cut(s)	20, 1189
BsiHKCI	CYCGRG	1 cut(s)	402
BsiSI	CCGG	1 cut(s)	1539
BsiWI	CGTACG	1 cut(s)	572
BslFI	GGGAC	3 cut(s)	105, 310, 1053
BslI	CCNNNNNNNGG	6 cut(s)	45, 132, 423, 424, 465, 1154
BsmAI	GTCTC	1 cut(s)	1214
BsmFI	GGGAC	3 cut(s)	105, 310, 1053
BsmI	GAATGC	2 cut(s)	370, 817
BsnI	GGCC	5 cut(s)	49, 136, 249, 407, 442
Bso31I	GGTCTC	1 cut(s)	1214
BsoBI	CYCGRG	1 cut(s)	402
Bsp119I	TTCGAA	1 cut(s)	85
Bsp120I	GGGCCC	1 cut(s)	405
Bsp1286I	GDGCHC	4 cut(s)	20, 409, 1189, 1334
Bsp143I	GATC	5 cut(s)	3, 265, 648, 967, 1006
BspACI	CCGC	4 cut(s)	73, 255, 364, 1118
BspANI	GGCC	5 cut(s)	49, 136, 249, 407, 442
BspLI	GGNNCC	7 cut(s)	259, 267, 407, 562, 829, 1041, 1432
BspPI	GGATC	4 cut(s)	260, 273, 656, 1014
BspT104I	TTCGAA	1 cut(s)	85
BspT107I	GGYRCC	3 cut(s)	257, 827, 1430
BspTI	CTTAAG	1 cut(s)	128
BspTNI	GGTCTC	1 cut(s)	1214
BsrDI	GCAATG	1 cut(s)	918
BsrFI	RCCGGY	1 cut(s)	1538
BsrI	ACTGG	2 cut(s)	250, 427
BssAI	RCCGGY	1 cut(s)	1538
BssECI	CCNNGG	6 cut(s)	30, 261, 393, 845, 1043, 1434
BssMI	GATC	5 cut(s)	3, 265, 648, 967, 1006
BssSI	CACGAG	1 cut(s)	200
BssT1I	CCWWGG	2 cut(s)	30, 393
Bst2BI	CACGAG	1 cut(s)	200
Bst2UI	CCWGG	2 cut(s)	263, 846
Bst4CI	ACNGT	3 cut(s)	827, 1435, 1528
BstAFI	CTTAAG	1 cut(s)	128
BstAPI	GCANNNNNTGC	3 cut(s)	812, 1178, 1346
BstBI	TTCGAA	1 cut(s)	85
BstC8I	GCNNGC	6 cut(s)	183, 364, 368, 500, 1174, 1540
BstDSI	CCRYGG	1 cut(s)	1434
BstF5I	GGATG	7 cut(s)	150, 225, 700, 730, 1049, 1134, 1282
BstH2I	RGCGCY	1 cut(s)	1545
BstHHI	GCGC	3 cut(s)	225, 1308, 1544
BstKTI	GATC	5 cut(s)	6, 268, 651, 970, 1009
BstMAI	GTCTC	1 cut(s)	1214
BstMBI	GATC	5 cut(s)	3, 265, 648, 967, 1006
BstMWI	GCNNNNNNNGC	8 cut(s)	398, 583, 704, 812, 956, 1099, 1178, 1346
BstNI	CCWGG	2 cut(s)	263, 846
BstSCI	CCNGG	2 cut(s)	261, 844
BstSLI	GKGCMC	2 cut(s)	409, 1189
BstV1I	GCAGC	2 cut(s)	481, 837
BstX2I	RGATCY	1 cut(s)	265
BstXI	CCANNNNNNTGG	2 cut(s)	925, 1029
BstYI	RGATCY	1 cut(s)	265
Bsu36I	CCTNAGG	2 cut(s)	112, 270
BsuI	GTATCC	1 cut(s)	1006
BsuRI	GGCC	5 cut(s)	49, 136, 249, 407, 442
BtgI	CCRYGG	1 cut(s)	1434
BtsCI	GGATG	7 cut(s)	150, 225, 700, 730, 1049, 1134, 1282
BtsI	GCAGTG	3 cut(s)	813, 945, 1176
BtsIMutI	CAGTG	7 cut(s)	50, 707, 813, 945, 1104, 1176, 1194
Cac8I	GCNNGC	6 cut(s)	183, 364, 368, 500, 1174, 1540
CaiI	CAGNNNCTG	1 cut(s)	1346
CfoI	GCGC	3 cut(s)	225, 1308, 1544
Cfr10I	RCCGGY	1 cut(s)	1538
Cfr13I	GGNCC	3 cut(s)	405, 406, 1040
CseI	GACGC	1 cut(s)	1517
Csp6I	GTAC	4 cut(s)	148, 258, 573, 1524
CspCI	CAANNNNNGTGG	2 cut(s)	298, 333
CviAII	CATG	7 cut(s)	652, 670, 691, 751, 971, 1054, 1094
CviQI	GTAC	4 cut(s)	148, 258, 573, 1524
DpnI	GATC	5 cut(s)	5, 267, 650, 969, 1008
DpnII	GATC	5 cut(s)	3, 265, 648, 967, 1006
DrdI	GACNNNNNNGTC	1 cut(s)	671
DseDI	GACNNNNNNGTC	1 cut(s)	671
EaeI	YGGCCR	1 cut(s)	247
Ecl136II	GAGCTC	1 cut(s)	18
Eco130I	CCWWGG	2 cut(s)	30, 393
Eco147I	AGGCCT	1 cut(s)	136
Eco24I	GRGCYC	3 cut(s)	20, 409, 1334
Eco31I	GGTCTC	1 cut(s)	1214
Eco32I	GATATC	2 cut(s)	453, 871
Eco47I	GGWCC	1 cut(s)	1040
Eco53kI	GAGCTC	1 cut(s)	18
Eco57I	CTGAAG	2 cut(s)	1401, 1482
Eco81I	CCTNAGG	2 cut(s)	112, 270
Eco88I	CYCGRG	1 cut(s)	402
EcoICRI	GAGCTC	1 cut(s)	18
EcoO109I	RGGNCCY	1 cut(s)	406
EcoRII	CCWGG	2 cut(s)	261, 844
EcoRV	GATATC	2 cut(s)	453, 871
EcoT14I	CCWWGG	2 cut(s)	30, 393
EcoT38I	GRGCYC	3 cut(s)	20, 409, 1334
ErhI	CCWWGG	2 cut(s)	30, 393
FaeI	CATG	7 cut(s)	655, 673, 694, 754, 974, 1057, 1097
FaqI	GGGAC	3 cut(s)	105, 310, 1053
FatI	CATG	7 cut(s)	651, 669, 690, 750, 970, 1053, 1093
FauI	CCCGC	1 cut(s)	371
FauNDI	CATATG	1 cut(s)	618
FbaI	TGATCA	2 cut(s)	3, 967
FblI	GTMKAC	1 cut(s)	1233
Fnu4HI	GCNGC	2 cut(s)	470, 851
FokI	GGATG	7 cut(s)	137, 212, 707, 737, 1036, 1141, 1289
FriOI	GRGCYC	3 cut(s)	20, 409, 1334
Fsp4HI	GCNGC	2 cut(s)	470, 851
FspBI	CTAG	2 cut(s)	774, 1313
GlaI	GCGC	3 cut(s)	224, 1307, 1543
GluI	GCNGC	2 cut(s)	470, 851
GsaI	CCCAGC	1 cut(s)	331
HaeII	RGCGCY	1 cut(s)	1545
HaeIII	GGCC	5 cut(s)	49, 136, 249, 407, 442
HapII	CCGG	1 cut(s)	1539
HgaI	GACGC	1 cut(s)	1517
HhaI	GCGC	3 cut(s)	225, 1308, 1544
Hin1II	CATG	7 cut(s)	655, 673, 694, 754, 974, 1057, 1097
Hin6I	GCGC	3 cut(s)	223, 1306, 1542
HinP1I	GCGC	3 cut(s)	223, 1306, 1542
HindIII	AAGCTT	1 cut(s)	500
HinfI	GANTC	4 cut(s)	413, 800, 841, 1160
HpaII	CCGG	1 cut(s)	1539
HphI	GGTGA	4 cut(s)	668, 1012, 1449, 1559
Hpy166II	GTNNAC	3 cut(s)	1187, 1234, 1457
Hpy188I	TCNGA	4 cut(s)	155, 438, 1396, 1477
Hpy188III	TCNNGA	6 cut(s)	173, 506, 541, 758, 769, 1010
Hpy8I	GTNNAC	3 cut(s)	1187, 1234, 1457
Hpy99I	CGWCG	1 cut(s)	199
HpyAV	CCTTC	6 cut(s)	65, 239, 1070, 1210, 1240, 1255
HpyCH4III	ACNGT	3 cut(s)	827, 1435, 1528
HpyF10VI	GCNNNNNNNGC	8 cut(s)	398, 583, 704, 812, 956, 1099, 1178, 1346
Hsp92II	CATG	7 cut(s)	655, 673, 694, 754, 974, 1057, 1097
HspAI	GCGC	3 cut(s)	223, 1306, 1542
KpnI	GGTACC	1 cut(s)	261
KroI	GCCGGC	1 cut(s)	1538
KroNI	GCCGGC	1 cut(s)	1540
Ksp22I	TGATCA	2 cut(s)	3, 967
Kzo9I	GATC	5 cut(s)	3, 265, 648, 967, 1006
LmnI	GCTCC	1 cut(s)	625
Lsp1109I	GCAGC	2 cut(s)	481, 837
LweI	GCATC	4 cut(s)	234, 685, 1058, 1594
MaeI	CTAG	2 cut(s)	774, 1313
MaeIII	GTNAC	2 cut(s)	274, 673
MalI	GATC	5 cut(s)	5, 267, 650, 969, 1008
MboI	GATC	5 cut(s)	3, 265, 648, 967, 1006
MflI	RGATCY	1 cut(s)	265
MhlI	GDGCHC	4 cut(s)	20, 409, 1189, 1334
MlsI	TGGCCA	1 cut(s)	249
MluCI	AATT	4 cut(s)	64, 930, 1064, 1082
MluNI	TGGCCA	1 cut(s)	249
MlyI	GAGTC	1 cut(s)	794
Mox20I	TGGCCA	1 cut(s)	249
MroNI	GCCGGC	1 cut(s)	1538
MscI	TGGCCA	1 cut(s)	249
MseI	TTAA	5 cut(s)	60, 129, 219, 836, 990
MslI	CAYNNNNRTG	2 cut(s)	950, 1275
Msp20I	TGGCCA	1 cut(s)	249
MspA1I	CMGCKG	2 cut(s)	255, 377
MspCI	CTTAAG	1 cut(s)	128
MspI	CCGG	1 cut(s)	1539
MspR9I	CCNGG	2 cut(s)	263, 846
Mva1269I	GAATGC	2 cut(s)	370, 817
MvaI	CCWGG	2 cut(s)	263, 846
MwoI	GCNNNNNNNGC	8 cut(s)	398, 583, 704, 812, 956, 1099, 1178, 1346
NaeI	GCCGGC	1 cut(s)	1540
NdeI	CATATG	1 cut(s)	618
NdeII	GATC	5 cut(s)	3, 265, 648, 967, 1006
NgoMIV	GCCGGC	1 cut(s)	1538
NlaIII	CATG	7 cut(s)	655, 673, 694, 754, 974, 1057, 1097
NlaIV	GGNNCC	7 cut(s)	259, 267, 407, 562, 829, 1041, 1432
NmuCI	GTSAC	1 cut(s)	673
NspV	TTCGAA	1 cut(s)	85
OliI	CACNNNNGTG	1 cut(s)	950
PceI	AGGCCT	1 cut(s)	136
PctI	GAATGC	2 cut(s)	370, 817
PdiI	GCCGGC	1 cut(s)	1540
PfeI	GAWTC	3 cut(s)	413, 841, 1160
Pfl23II	CGTACG	1 cut(s)	572
PkrI	GCNGC	2 cut(s)	471, 852
PleI	GAGTC	1 cut(s)	794
PpsI	GAGTC	1 cut(s)	794
PsiI	TTATAA	1 cut(s)	780
Psp124BI	GAGCTC	1 cut(s)	20
Psp6I	CCWGG	2 cut(s)	261, 844
PspFI	CCCAGC	1 cut(s)	327
PspGI	CCWGG	2 cut(s)	261, 844
PspLI	CGTACG	1 cut(s)	572
PspN4I	GGNNCC	7 cut(s)	259, 267, 407, 562, 829, 1041, 1432
PspOMI	GGGCCC	1 cut(s)	405
PspPI	GGNCC	3 cut(s)	405, 406, 1040
PstNI	CAGNNNCTG	1 cut(s)	1346
PsuI	RGATCY	1 cut(s)	265
PvuII	CAGCTG	1 cut(s)	377
RsaI	GTAC	4 cut(s)	149, 259, 574, 1525
RsaNI	GTAC	4 cut(s)	148, 258, 573, 1524
RseI	CAYNNNNRTG	2 cut(s)	950, 1275
SacI	GAGCTC	1 cut(s)	20
SaqAI	TTAA	5 cut(s)	60, 129, 219, 836, 990
SatI	GCNGC	2 cut(s)	470, 851
Sau3AI	GATC	5 cut(s)	3, 265, 648, 967, 1006
Sau96I	GGNCC	3 cut(s)	405, 406, 1040
SchI	GAGTC	1 cut(s)	794
ScrFI	CCNGG	2 cut(s)	263, 846
SduI	GDGCHC	4 cut(s)	20, 409, 1189, 1334
SfaNI	GCATC	4 cut(s)	234, 685, 1058, 1594
SfuI	TTCGAA	1 cut(s)	85
SinI	GGWCC	1 cut(s)	1040
SmiMI	CAYNNNNRTG	2 cut(s)	950, 1275
SmlI	CTYRAG	3 cut(s)	128, 506, 758
SmoI	CTYRAG	3 cut(s)	128, 506, 758
SpeI	ACTAGT	1 cut(s)	773
Sse9I	AATT	4 cut(s)	64, 930, 1064, 1082
SseBI	AGGCCT	1 cut(s)	136
SsiI	CCGC	4 cut(s)	73, 255, 364, 1118
SspMI	CTAG	2 cut(s)	774, 1313
SstI	GAGCTC	1 cut(s)	20
StuI	AGGCCT	1 cut(s)	136
StyD4I	CCNGG	2 cut(s)	261, 844
StyI	CCWWGG	2 cut(s)	30, 393
TaaI	ACNGT	3 cut(s)	827, 1435, 1528
TaqI	TCGA	2 cut(s)	85, 873
TaqII	GACCGA	2 cut(s)	318, 1057
TasI	AATT	4 cut(s)	64, 930, 1064, 1082
TatI	WGTACW	1 cut(s)	147
TfiI	GAWTC	3 cut(s)	413, 841, 1160
Tru1I	TTAA	5 cut(s)	60, 129, 219, 836, 990
Tru9I	TTAA	5 cut(s)	60, 129, 219, 836, 990
TscAI	CASTG	7 cut(s)	50, 707, 813, 952, 1111, 1183, 1201
TseFI	GTSAC	1 cut(s)	673
TseI	GCWGC	2 cut(s)	469, 850
Tsp45I	GTSAC	1 cut(s)	673
TspDTI	ATGAA	3 cut(s)	507, 1070, 1293
TspRI	CASTG	7 cut(s)	50, 707, 813, 952, 1111, 1183, 1201
Vha464I	CTTAAG	1 cut(s)	128
VneI	GTGCAC	1 cut(s)	1185
VpaK11BI	GGWCC	1 cut(s)	1040
XcmI	CCANNNNNNNNNTGG	1 cut(s)	952
XmiI	GTMKAC	1 cut(s)	1233
XspI	CTAG	2 cut(s)	774, 1313

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.