Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0010475

Physical Location & Seq

Genomic Coordinates Forward (+)

44900244 .. 44902888

Gene Structure 2645 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00251050.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1890 bp

ATGAAAAGTCTCCCTCACCCTCGAGTTGATGGCCCAAACCCTCCGAATAAGAGAGGCGAAGTGAGGAGTGAGAGTCAGAACCCTCTAGGTGGCCGCCCTCTCGCTCAACTGTCCAAATCCAAAAGGGTTTTGCTCCCAGCCGAAATCAAACTCCAAGAAAATGTCTTCAGACGACGAAAAACCCCAGTCAAGGCCATATCAGTGAAGGATAGTGAGAAGAAGAAGAAGCACAGAGAGAAAGACAATGATGTGGACCAAGACTACATGATCAAGCCCCAGAGCTCGACTCCCTCCCTGGACACATCTCAGTGGCCCATTCTTCTCAAGAACTATGACCGCCTCAATGTTCGAACTGGACATTACACACCTCTTCCCTCGGGGTACTCCCCGCTCAAGCGGCCTCTTGCAGAGTATATCAGATATGGTGTTCTCAATCTTGACAAGCCCGCAAACCCCTCTTCCCACGAGGTTGTTGCTTGGATTAAACGCATCCTTCGTGTTGAGAAGACTGGTCACAGCGGTACTCTTGATCCTAAGGTTACAGGTAATCTCATTGTCTGTATTGACCGGGCAACACGACTTGTCAAGTCTCAGCAAGGTGCAGGAAAAGAATATGTTTGTGTTGCACGCCTGCATTCAGCTGTGCCTGATGTTGCCAAGGTGGCCCGGGCACTTGAATCCCTCACTGGCGCTGTCTTTCAGAGGCCACCTTTGATATCTGCAGTCAAGAGGCAGCTTAGGATTAGGACCATCTATGAAAGCAAGCTTCTTGAGTATGATGCGGAGAAGCATCTGGTTGTTTTCTGGATTTCCTGTGAGGCAGGTACCTATGTGCGAACACTGTGTGTGCATTTGGGTCTGCTTCTTGGTGTGGGAGGGCATATGCAGGAGCTTAGGAGGGTCAGGTCTGGGACTATGGGTGAGAAAGAGAACATGGTTACCATGCATGATGTCATGGATGCTCAATGGGCTTATGATACTAATAGGGATGAGAGTTATTTGAGGAGGGTTATTATGCCACTTGAGGTGCTTCTGACCAGTCATAAGAGATTAGTTGTCAAGGACTCAGCAGTGAATGCCATTTGCTATGGTGCCAAGTTGATGATTCCTGGGCTGCTGAGGTTTGAGAATGAAATTGAGGTAGGAGAGGAAGTTGTGCTTATGACTACCAAAGGTGAAGCAATTGCTTTGGGAATTGCTGAGATGACCACTGCTGTGATGGCAACTTGCGATCATGGTGTGGTGGCAAAGATTAAGAGGGTGGTGATGGATAGGGATACTTATCCTAGGAAGTGGGGTTTGGGGCCAAGGGCATCCATGAAGAAGAAATTGATTGCAGAAGGGAAATTGGATAAGCATGGGAAGCCTACTGAGAATACTCCTCCAGAATGGGGCCGGAATTTGGTTTTACCGACAGGTGGAGATTCGGTTATTGCAAGTGCTGCTGCTGAGCTTGCTGCTGCACCTGCTGTTGCCATTGAGACAACTTCTGTTTCAGAGAAGAAAGAGAAGAAGAAACGCAAACAAGAGGAAATAACTGACAGTCCTGTTCCAGTTCCTGCAAAGAAAAGCAAAGGTGAAGAAGTAGCTGAGGCTGAAGTTGAGAAGACAGAAAAGAAGAAGAAAAAGAAAAAGGATAAAGACAATGGTGTTTTGGATTCTTCAGATGGGGAGAAGTCTGTGAAGGTGAAGAAGCACAAAGATAAAGAAGAAGCTGGTTCACCTGCAACTGAAAAGTCTGAAAAGAAGAAAAAGAAGAAAAACAGAGAGGCTGAGGATCATGGTGCTGATGCTGTCACTGATAATGGGAAGGGTGATGGTGAGGCTGATAAGAGTGAGAAAAAGAAGAAGAAGAAGAAAAAGGATAAATCGGATGCCGACAAAGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000154 GO:0000495 GO:0001522 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005732 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009506 GO:0009982 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0016072 GO:0016073 GO:0016074 GO:0016556 GO:0016853 GO:0016866 GO:0022613 GO:0030054 GO:0031118 GO:0031120 GO:0031123 GO:0031126 GO:0031429 GO:0031974 GO:0031981 GO:0032991 GO:0033979 GO:0034470 GO:0034641 GO:0034660 GO:0034964 GO:0040031 GO:0042254 GO:0043144 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043628 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0055044 GO:0070013 GO:0071704 GO:0071840 GO:0072588 GO:0090304 GO:1901360 GO:1902494 GO:1990481 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03008 map03008

Pfam Domains

Protein Families

DKCLD PUA TruB_C_2 TruB_N

Protein Analysis

629

Amino Acids

69.93

Weight (kDa)

9.59

Isoelectric Point (pI)

39.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DKCLD	PF08068	101 - 158	5.7e-31	DKCLD (NUC011) domain
TruB_N	PF01509	162 - 278	1.1e-20	TruB family pseudouridylate synthase (N terminal domain)
TruB_C_2	PF16198	279 - 345	1.2e-26	tRNA pseudouridylate synthase B C-terminal domain
PUA	PF01472	349 - 422	1.6e-23	PUA domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0010475)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G57150
fragaria_vesca	FvH4_5g20900 FvH4_5g20900 FvH4_5g20900
malus_domestica	MD06G1043100.v1.1 MD16G1267700.v1.1 MD16G1267900.v1.1
prunus_persica	Prupe.1G572500_v2.0.a1 Prupe.5G050000_v2.0.a1
pyrus_communis	pycom06g03610 pycom16g23720
rosa_chinensis	RchiOBHm_Chr7g0207911
rosa_laevigata	RLG00000003276
rosa_multiflora	Rmu_sc0006577.1_g000004
rosa_roxburghii	Rroxscaffold_3G00251050
rosa_rugosa	Rorug07G0100200
rosa_samantha	Rh7AG233600 Rh7BG229200 Rh7CG248900 Rh7DG239600
rosa_wichuraiana	Rw0G001290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	1474, 1732
Acc36I	ACCTGC	3 cut(s)	812, 1474, 1732
Acc65I	GGTACC	1 cut(s)	824
AccB1I	GGYRCC	2 cut(s)	824, 1091
AccBSI	CCGCTC	1 cut(s)	391
AciI	CCGC	7 cut(s)	94, 337, 389, 397, 447, 519, 782
AclWI	GGATC	2 cut(s)	524, 1785
AcoI	YGGCCR	1 cut(s)	91
AcsI	RAATTY	1 cut(s)	1399
AcuI	CTGAAG	3 cut(s)	151, 1617, 1647
AdeI	CACNNNGTG	1 cut(s)	845
AfaI	GTAC	3 cut(s)	383, 523, 826
AfiI	CCNNNNNNNGG	5 cut(s)	89, 190, 1391, 1402, 1418
AgsI	TTSAA	1 cut(s)	677
AjnI	CCWGG	2 cut(s)	294, 1108
AjuI	GAANNNNNNNTTGG	2 cut(s)	1283, 1315
AleI	CACNNNNGTG	1 cut(s)	1214
AluBI	AGCT	8 cut(s)	282, 641, 736, 766, 892, 1453, 1589, 1715
AluI	AGCT	8 cut(s)	282, 641, 736, 766, 892, 1453, 1589, 1715
Alw21I	GWGCWC	1 cut(s)	284
Alw26I	GTCTC	3 cut(s)	14, 594, 1475
AlwI	GGATC	2 cut(s)	524, 1785
AlwNI	CAGNNNCTG	1 cut(s)	1559
Ama87I	CYCGRG	3 cut(s)	21, 376, 666
AoxI	GGCC	9 cut(s)	31, 91, 192, 311, 398, 663, 704, 1304, 1393
ApeKI	GCWGC	6 cut(s)	733, 1114, 1442, 1445, 1457, 1460
ApoI	RAATTY	1 cut(s)	1399
Asp718I	GGTACC	1 cut(s)	824
AspA2I	CCTAGG	1 cut(s)	1286
AspLEI	GCGC	1 cut(s)	692
AspS9I	GGNCC	7 cut(s)	32, 253, 312, 664, 747, 1304, 1393
AsuC2I	CCSGG	3 cut(s)	569, 667, 668
AsuHPI	GGTGA	9 cut(s)	8, 932, 1187, 1276, 1589, 1699, 1713, 1826, 1832
AsuII	TTCGAA	1 cut(s)	349
AvaI	CYCGRG	3 cut(s)	21, 376, 666
AvaII	GGWCC	2 cut(s)	253, 747
AvrII	CCTAGG	1 cut(s)	1286
AxyI	CCTNAGG	1 cut(s)	534
BaeGI	GKGCMC	1 cut(s)	673
BaeI	ACNNNNGTAYC	2 cut(s)	816, 849
BanI	GGYRCC	2 cut(s)	824, 1091
BanII	GRGCYC	1 cut(s)	284
BauI	CACGAG	1 cut(s)	464
BbsI	GAAGAC	3 cut(s)	157, 512, 1613
Bbv12I	GWGCWC	1 cut(s)	284
BbvCI	CCTCAGC	3 cut(s)	1118, 1590, 1773
BbvI	GCAGC	6 cut(s)	745, 1101, 1429, 1432, 1444, 1447
BccI	CCATC	6 cut(s)	23, 758, 1213, 1261, 1661, 1811
BcgI	CGANNNNNNTGC	2 cut(s)	455, 489
BciT130I	CCWGG	2 cut(s)	296, 1110
BciVI	GTATCC	1 cut(s)	1270
BclI	TGATCA	1 cut(s)	267
BcnI	CCSGG	3 cut(s)	569, 667, 668
BcoDI	GTCTC	3 cut(s)	14, 594, 1475
BfaI	CTAG	2 cut(s)	86, 1287
BfmI	CTRYAG	1 cut(s)	720
BfoI	RGCGCY	1 cut(s)	693
BfuAI	ACCTGC	3 cut(s)	812, 1474, 1732
BfuI	GTATCC	1 cut(s)	1270
BglI	GCCNNNNNGGC	1 cut(s)	662
BisI	GCNGC	8 cut(s)	94, 398, 734, 1115, 1443, 1446, 1458, 1461
BlnI	CCTAGG	1 cut(s)	1286
BlpI	GCTNAGC	1 cut(s)	1449
BlsI	GCNGC	8 cut(s)	95, 399, 735, 1116, 1444, 1447, 1459, 1462
Bme1390I	CCNGG	5 cut(s)	296, 569, 667, 668, 1110
Bme18I	GGWCC	2 cut(s)	253, 747
BmeT110I	CYCGRG	3 cut(s)	21, 376, 666
BmgT120I	GGNCC	7 cut(s)	32, 253, 312, 664, 747, 1304, 1393
BmiI	GGNNCC	4 cut(s)	826, 1093, 1305, 1394
BmrFI	CCNGG	5 cut(s)	296, 569, 667, 668, 1110
BmrI	ACTGGG	1 cut(s)	179
BmsI	GCATC	7 cut(s)	498, 769, 799, 949, 1322, 1780, 1864
BmuI	ACTGGG	1 cut(s)	179
BpiI	GAAGAC	3 cut(s)	157, 512, 1613
BplI	GAGNNNNNCTC	2 cut(s)	271, 303
BpmI	CTGGAG	1 cut(s)	1368
Bpu10I	CCTNAGC	5 cut(s)	737, 893, 1118, 1590, 1773
Bpu1102I	GCTNAGC	1 cut(s)	1449
Bpu14I	TTCGAA	1 cut(s)	349
BpuEI	CTTGAG	4 cut(s)	308, 377, 791, 1043
BpuMI	CCSGG	3 cut(s)	569, 667, 668
BsaBI	GATNNNNATC	1 cut(s)	1281
BsaJI	CCNNGG	7 cut(s)	294, 375, 657, 666, 1109, 1286, 1307
BsaXI	ACNNNNNCTCC	6 cut(s)	58, 88, 1137, 1167, 1413, 1443
Bsc4I	CCNNNNNNNGG	5 cut(s)	89, 190, 1391, 1402, 1418
Bse1I	ACTGG	6 cut(s)	185, 358, 514, 691, 1038, 1553
Bse21I	CCTNAGG	1 cut(s)	534
Bse8I	GATNNNNATC	1 cut(s)	1281
BseBI	CCWGG	2 cut(s)	296, 1110
BseDI	CCNNGG	7 cut(s)	294, 375, 657, 666, 1109, 1286, 1307
BseGI	GGATG	5 cut(s)	489, 964, 994, 1313, 1879
BseJI	GATNNNNATC	1 cut(s)	1281
BseLI	CCNNNNNNNGG	5 cut(s)	89, 190, 1391, 1402, 1418
BseMII	CTCAG	9 cut(s)	320, 605, 1080, 1109, 1191, 1362, 1440, 1581, 1764
BseNI	ACTGG	6 cut(s)	185, 358, 514, 691, 1038, 1553
BseRI	GAGGAG	3 cut(s)	79, 1018, 1371
BseSI	GKGCMC	1 cut(s)	673
BseXI	GCAGC	6 cut(s)	745, 1101, 1429, 1432, 1444, 1447
BseYI	CCCAGC	1 cut(s)	136
BsgI	GTGCAG	2 cut(s)	621, 1446
BshFI	GGCC	9 cut(s)	33, 93, 194, 313, 400, 665, 706, 1306, 1395
BshNI	GGYRCC	2 cut(s)	824, 1091
BsiHKAI	GWGCWC	1 cut(s)	284
BsiHKCI	CYCGRG	3 cut(s)	21, 376, 666
BsiSI	CCGG	3 cut(s)	568, 667, 1396
BslFI	GGGAC	1 cut(s)	925
BslI	CCNNNNNNNGG	5 cut(s)	89, 190, 1391, 1402, 1418
BsmAI	GTCTC	3 cut(s)	14, 594, 1475
BsmFI	GGGAC	1 cut(s)	925
BsmI	GAATGC	2 cut(s)	634, 1081
BsnI	GGCC	9 cut(s)	33, 93, 194, 313, 400, 665, 706, 1306, 1395
BsoBI	CYCGRG	3 cut(s)	21, 376, 666
Bsp119I	TTCGAA	1 cut(s)	349
Bsp1286I	GDGCHC	2 cut(s)	284, 673
Bsp143I	GATC	4 cut(s)	267, 529, 1231, 1777
Bsp1720I	GCTNAGC	1 cut(s)	1449
BspACI	CCGC	7 cut(s)	94, 337, 389, 397, 447, 519, 782
BspANI	GGCC	9 cut(s)	33, 93, 194, 313, 400, 665, 706, 1306, 1395
BspCNI	CTCAG	9 cut(s)	319, 604, 1079, 1110, 1192, 1363, 1441, 1582, 1765
BspLI	GGNNCC	4 cut(s)	826, 1093, 1305, 1394
BspMAI	CTGCAG	1 cut(s)	724
BspMI	ACCTGC	3 cut(s)	812, 1474, 1732
BspPI	GGATC	2 cut(s)	524, 1785
BspT104I	TTCGAA	1 cut(s)	349
BspT107I	GGYRCC	2 cut(s)	824, 1091
BsrBI	CCGCTC	1 cut(s)	391
BsrI	ACTGG	6 cut(s)	185, 358, 514, 691, 1038, 1553
BssECI	CCNNGG	7 cut(s)	294, 375, 657, 666, 1109, 1286, 1307
BssMI	GATC	4 cut(s)	267, 529, 1231, 1777
BssSI	CACGAG	1 cut(s)	464
BssT1I	CCWWGG	3 cut(s)	657, 1286, 1307
Bst2BI	CACGAG	1 cut(s)	464
Bst2UI	CCWGG	2 cut(s)	296, 1110
Bst4CI	ACNGT	3 cut(s)	111, 843, 1544
Bst6I	CTCTTC	2 cut(s)	375, 463
BstAPI	GCANNNNNTGC	2 cut(s)	1076, 1442
BstBI	TTCGAA	1 cut(s)	349
BstC8I	GCNNGC	5 cut(s)	447, 628, 632, 764, 1455
BstEII	GGTNACC	1 cut(s)	937
BstF5I	GGATG	5 cut(s)	489, 964, 994, 1313, 1879
BstH2I	RGCGCY	1 cut(s)	693
BstHHI	GCGC	1 cut(s)	692
BstKTI	GATC	4 cut(s)	270, 532, 1234, 1780
BstMAI	GTCTC	3 cut(s)	14, 594, 1475
BstMBI	GATC	4 cut(s)	267, 529, 1231, 1777
BstMWI	GCNNNNNNNGC	9 cut(s)	397, 662, 968, 1076, 1220, 1363, 1442, 1454, 1466
BstNI	CCWGG	2 cut(s)	296, 1110
BstPI	GGTNACC	1 cut(s)	937
BstSCI	CCNGG	5 cut(s)	294, 567, 665, 666, 1108
BstSFI	CTRYAG	1 cut(s)	720
BstSLI	GKGCMC	1 cut(s)	673
BstV1I	GCAGC	6 cut(s)	745, 1101, 1429, 1432, 1444, 1447
BstV2I	GAAGAC	3 cut(s)	157, 512, 1613
Bsu36I	CCTNAGG	1 cut(s)	534
BsuI	GTATCC	1 cut(s)	1270
BsuRI	GGCC	9 cut(s)	33, 93, 194, 313, 400, 665, 706, 1306, 1395
BtsCI	GGATG	5 cut(s)	489, 964, 994, 1313, 1879
BtsI	GCAGTG	2 cut(s)	1077, 1209
BtsIMutI	CAGTG	7 cut(s)	207, 314, 684, 839, 1077, 1209, 1797
BveI	ACCTGC	3 cut(s)	812, 1474, 1732
Cac8I	GCNNGC	5 cut(s)	447, 628, 632, 764, 1455
CaiI	CAGNNNCTG	1 cut(s)	1559
CfoI	GCGC	1 cut(s)	692
Cfr13I	GGNCC	7 cut(s)	32, 253, 312, 664, 747, 1304, 1393
Cfr9I	CCCGGG	1 cut(s)	666
Csp6I	GTAC	3 cut(s)	382, 522, 825
CviAII	CATG	9 cut(s)	265, 934, 943, 947, 955, 1235, 1318, 1358, 1781
CviQI	GTAC	3 cut(s)	382, 522, 825
DpnI	GATC	4 cut(s)	269, 531, 1233, 1779
DpnII	GATC	4 cut(s)	267, 529, 1231, 1777
DraIII	CACNNNGTG	1 cut(s)	845
EaeI	YGGCCR	1 cut(s)	91
Eam1104I	CTCTTC	2 cut(s)	375, 463
EarI	CTCTTC	2 cut(s)	375, 463
Ecl136II	GAGCTC	1 cut(s)	282
Eco130I	CCWWGG	3 cut(s)	657, 1286, 1307
Eco24I	GRGCYC	1 cut(s)	284
Eco32I	GATATC	1 cut(s)	717
Eco47I	GGWCC	2 cut(s)	253, 747
Eco53kI	GAGCTC	1 cut(s)	282
Eco57I	CTGAAG	3 cut(s)	151, 1617, 1647
Eco81I	CCTNAGG	1 cut(s)	534
Eco88I	CYCGRG	3 cut(s)	21, 376, 666
Eco91I	GGTNACC	1 cut(s)	937
EcoICRI	GAGCTC	1 cut(s)	282
EcoO65I	GGTNACC	1 cut(s)	937
EcoRII	CCWGG	2 cut(s)	294, 1108
EcoRV	GATATC	1 cut(s)	717
EcoT14I	CCWWGG	3 cut(s)	657, 1286, 1307
EcoT22I	ATGCAT	1 cut(s)	948
EcoT38I	GRGCYC	1 cut(s)	284
ErhI	CCWWGG	3 cut(s)	657, 1286, 1307
FaeI	CATG	9 cut(s)	268, 937, 946, 950, 958, 1238, 1321, 1361, 1784
FalI	AAGNNNNNCTT	2 cut(s)	1143, 1175
FaqI	GGGAC	1 cut(s)	925
FatI	CATG	9 cut(s)	264, 933, 942, 946, 954, 1234, 1317, 1357, 1780
FauI	CCCGC	2 cut(s)	396, 454
FauNDI	CATATG	1 cut(s)	882
FbaI	TGATCA	1 cut(s)	267
Fnu4HI	GCNGC	8 cut(s)	94, 398, 734, 1115, 1443, 1446, 1458, 1461
FokI	GGATG	5 cut(s)	476, 971, 1001, 1300, 1886
FriOI	GRGCYC	1 cut(s)	284
Fsp4HI	GCNGC	8 cut(s)	94, 398, 734, 1115, 1443, 1446, 1458, 1461
FspBI	CTAG	2 cut(s)	86, 1287
GlaI	GCGC	1 cut(s)	691
GluI	GCNGC	8 cut(s)	94, 398, 734, 1115, 1443, 1446, 1458, 1461
GsaI	CCCAGC	1 cut(s)	140
GsuI	CTGGAG	1 cut(s)	1368
HaeII	RGCGCY	1 cut(s)	693
HaeIII	GGCC	9 cut(s)	33, 93, 194, 313, 400, 665, 706, 1306, 1395
HapII	CCGG	3 cut(s)	568, 667, 1396
HhaI	GCGC	1 cut(s)	692
Hin1II	CATG	9 cut(s)	268, 937, 946, 950, 958, 1238, 1321, 1361, 1784
Hin6I	GCGC	1 cut(s)	690
HinP1I	GCGC	1 cut(s)	690
HindIII	AAGCTT	1 cut(s)	764
HinfI	GANTC	7 cut(s)	73, 286, 677, 1064, 1105, 1424, 1658
HpaII	CCGG	3 cut(s)	568, 667, 1396
HphI	GGTGA	9 cut(s)	8, 932, 1187, 1276, 1589, 1699, 1713, 1826, 1832
Hpy166II	GTNNAC	2 cut(s)	253, 1721
Hpy188III	TCNNGA	7 cut(s)	325, 437, 527, 727, 770, 805, 1385
Hpy8I	GTNNAC	2 cut(s)	253, 1721
Hpy99I	CGWCG	1 cut(s)	177
HpyAV	CCTTC	5 cut(s)	199, 503, 1334, 1678, 1804
HpyCH4III	ACNGT	3 cut(s)	111, 843, 1544
HpyF10VI	GCNNNNNNNGC	9 cut(s)	397, 662, 968, 1076, 1220, 1363, 1442, 1454, 1466
Hsp92II	CATG	9 cut(s)	268, 937, 946, 950, 958, 1238, 1321, 1361, 1784
HspAI	GCGC	1 cut(s)	690
KpnI	GGTACC	1 cut(s)	828
Ksp22I	TGATCA	1 cut(s)	267
Kzo9I	GATC	4 cut(s)	267, 529, 1231, 1777
LmnI	GCTCC	2 cut(s)	138, 889
Lsp1109I	GCAGC	6 cut(s)	745, 1101, 1429, 1432, 1444, 1447
LweI	GCATC	7 cut(s)	498, 769, 799, 949, 1322, 1780, 1864
MaeI	CTAG	2 cut(s)	86, 1287
MaeIII	GTNAC	4 cut(s)	512, 538, 937, 1795
MalI	GATC	4 cut(s)	269, 531, 1233, 1779
MbiI	CCGCTC	1 cut(s)	391
MboI	GATC	4 cut(s)	267, 529, 1231, 1777
MfeI	CAATTG	1 cut(s)	1182
MhlI	GDGCHC	2 cut(s)	284, 673
MluCI	AATT	6 cut(s)	1134, 1182, 1194, 1328, 1346, 1399
MlyI	GAGTC	3 cut(s)	82, 280, 1058
Mph1103I	ATGCAT	1 cut(s)	948
MseI	TTAA	2 cut(s)	483, 1254
MslI	CAYNNNNRTG	3 cut(s)	200, 307, 1214
MspA1I	CMGCKG	2 cut(s)	519, 641
MspI	CCGG	3 cut(s)	568, 667, 1396
MspR9I	CCNGG	5 cut(s)	296, 569, 667, 668, 1110
MunI	CAATTG	1 cut(s)	1182
Mva1269I	GAATGC	2 cut(s)	634, 1081
MvaI	CCWGG	2 cut(s)	296, 1110
MwoI	GCNNNNNNNGC	9 cut(s)	397, 662, 968, 1076, 1220, 1363, 1442, 1454, 1466
NciI	CCSGG	3 cut(s)	569, 667, 668
NdeI	CATATG	1 cut(s)	882
NdeII	GATC	4 cut(s)	267, 529, 1231, 1777
NlaIII	CATG	9 cut(s)	268, 937, 946, 950, 958, 1238, 1321, 1361, 1784
NlaIV	GGNNCC	4 cut(s)	826, 1093, 1305, 1394
NmuCI	GTSAC	2 cut(s)	512, 1795
NsiI	ATGCAT	1 cut(s)	948
NspV	TTCGAA	1 cut(s)	349
OliI	CACNNNNGTG	1 cut(s)	1214
PaeR7I	CTCGAG	1 cut(s)	21
PaqCI	CACCTGC	2 cut(s)	1474, 1732
PcsI	WCGNNNNNNNCGW	1 cut(s)	493
PctI	GAATGC	2 cut(s)	634, 1081
PfeI	GAWTC	4 cut(s)	677, 1105, 1424, 1658
PkrI	GCNGC	8 cut(s)	95, 399, 735, 1116, 1444, 1447, 1459, 1462
PleI	GAGTC	3 cut(s)	81, 280, 1058
PpsI	GAGTC	3 cut(s)	81, 280, 1058
Psp124BI	GAGCTC	1 cut(s)	284
Psp6I	CCWGG	2 cut(s)	294, 1108
PspEI	GGTNACC	1 cut(s)	937
PspFI	CCCAGC	1 cut(s)	136
PspGI	CCWGG	2 cut(s)	294, 1108
PspN4I	GGNNCC	4 cut(s)	826, 1093, 1305, 1394
PspPI	GGNCC	7 cut(s)	32, 253, 312, 664, 747, 1304, 1393
PspXI	VCTCGAGB	1 cut(s)	21
PstI	CTGCAG	1 cut(s)	724
PstNI	CAGNNNCTG	1 cut(s)	1559
PvuII	CAGCTG	1 cut(s)	641
RsaI	GTAC	3 cut(s)	383, 523, 826
RsaNI	GTAC	3 cut(s)	382, 522, 825
RseI	CAYNNNNRTG	3 cut(s)	200, 307, 1214
SacI	GAGCTC	1 cut(s)	284
SaqAI	TTAA	2 cut(s)	483, 1254
SatI	GCNGC	8 cut(s)	94, 398, 734, 1115, 1443, 1446, 1458, 1461
Sau3AI	GATC	4 cut(s)	267, 529, 1231, 1777
Sau96I	GGNCC	7 cut(s)	32, 253, 312, 664, 747, 1304, 1393
SchI	GAGTC	3 cut(s)	82, 280, 1058
ScrFI	CCNGG	5 cut(s)	296, 569, 667, 668, 1110
SduI	GDGCHC	2 cut(s)	284, 673
SfaNI	GCATC	7 cut(s)	498, 769, 799, 949, 1322, 1780, 1864
SfcI	CTRYAG	1 cut(s)	720
Sfr274I	CTCGAG	1 cut(s)	21
SfuI	TTCGAA	1 cut(s)	349
SinI	GGWCC	2 cut(s)	253, 747
SlaI	CTCGAG	1 cut(s)	21
SmaI	CCCGGG	1 cut(s)	668
SmiMI	CAYNNNNRTG	3 cut(s)	200, 307, 1214
SmlI	CTYRAG	5 cut(s)	21, 323, 392, 770, 1022
SmoI	CTYRAG	5 cut(s)	21, 323, 392, 770, 1022
SrfI	GCCCGGGC	1 cut(s)	668
Sse9I	AATT	6 cut(s)	1134, 1182, 1194, 1328, 1346, 1399
SsiI	CCGC	7 cut(s)	94, 337, 389, 397, 447, 519, 782
SspMI	CTAG	2 cut(s)	86, 1287
SstI	GAGCTC	1 cut(s)	284
StyD4I	CCNGG	5 cut(s)	294, 567, 665, 666, 1108
StyI	CCWWGG	3 cut(s)	657, 1286, 1307
TaaI	ACNGT	3 cut(s)	111, 843, 1544
TaqI	TCGA	3 cut(s)	22, 284, 349
TasI	AATT	6 cut(s)	1134, 1182, 1194, 1328, 1346, 1399
TauI	GCSGC	2 cut(s)	96, 400
TfiI	GAWTC	4 cut(s)	677, 1105, 1424, 1658
Tru1I	TTAA	2 cut(s)	483, 1254
Tru9I	TTAA	2 cut(s)	483, 1254
TscAI	CASTG	7 cut(s)	207, 314, 691, 846, 1077, 1216, 1804
TseFI	GTSAC	2 cut(s)	512, 1795
TseI	GCWGC	6 cut(s)	733, 1114, 1442, 1445, 1457, 1460
Tsp45I	GTSAC	2 cut(s)	512, 1795
TspDTI	ATGAA	4 cut(s)	17, 771, 1146, 1334
TspMI	CCCGGG	1 cut(s)	666
TspRI	CASTG	7 cut(s)	207, 314, 691, 846, 1077, 1216, 1804
VpaK11BI	GGWCC	2 cut(s)	253, 747
XapI	RAATTY	1 cut(s)	1399
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1216
XhoI	CTCGAG	1 cut(s)	21
XmaI	CCCGGG	1 cut(s)	666
XmaJI	CCTAGG	1 cut(s)	1286
XspI	CTAG	2 cut(s)	86, 1287
Zsp2I	ATGCAT	1 cut(s)	948

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.