Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Contig00042

Orthogroup ID

OG0010475

Physical Location & Seq

Genomic Coordinates Forward (+)

151641 .. 154274

Gene Structure 2634 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw0G001290.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1734 bp

ATGTCTTCAGACGACGAAAAAACCCCAGTCAAGGCCATATCAGTGAAGGATAGTGAGAAGAAGAAAAAGAAGCACAGAGAGAAAGACAATGATGTGGACCAAGACTACATGATCAAGCCCCAGAGCTCGACTCCCTCCCTGGACACATCTCAGTGGCCCATTCTTCTCAAGAACTATGACCGCCTCAATGTTCGAACTGGACATTACACACCTCTTCCCTCGGGGTACTCCCCGCTCAAGCGGCCTCTTGCAGAGTATATCAGATATGGTGTTCTCAATCTTGACAAGCCCGCAAACCCTTCCTCCCATGAGGTTGTTGCTTGGATTAAACGCATCCTTCGTGTTGAGAAGACTGGTCACAGCGGTACTCTTGATCCTAAGGTTACAGGTAATCTCATTGTCTGTATTGACCGGGCAACACGACTTGTCAAGTCTCAGCAAGGTGCAGGAAAAGAGTATGTCTGTGTTGCACGCCTGCATTCAGCTGTGCCTGATGTTGCCAAGGTGGCCCGGGCACTTGAATCCCTCACTGGTGCTGTCTTTCAGAGGCCACCTTTGATATCTGCAGTCAAGAGGCAGCTTAGGATTAGGACCATCTATGAAAGCAAGCTTCTTGAGTATGATGCGGAGAAGCATCTGGTTGTTTTCTGGATTTCCTGTGAGGCTGGTACCTATGTGCGAACACTGTGTGTGCATTTGGGTCTGCTTCTTGGTGTGGGAGGGCATATGCAGGAGCTTAGGAGGGTCAGGTCTGGGACTATGGGTGAGAAAGAGAACATGGTTACCATGCATGATGTCATGGATGCTCAATGGGCTTATGATACTAATAGGGATGAGAGTTATTTGAGGAGGGTTATTATGCCACTTGAGGTGCTTCTGACCAGTCATAAGAGATTAGTTGTCAAGGACTCAGCAGTGAATGCCATTTGCTATGGTGCCAAGTTGATGATTCCTGGGCTGCTGAGGTTTGAGAATGAAATTGAGGTAGGAGAGGAAGTTGTGCTTATGACTACCAAAGGTGAAGCAATTGCTTTGGGAATTGCTGAGATGACCACTGCTGTGATGGCAACTTGTGATCATGGTGTGGTGGCAAAGATTAAGAGGGTGGTGATGGATAGGGATACTTATCCTAGGAAGTGGGGTTTGGGGCCAAGGGCATCCATGAAGAAGAAATTGATTGCAGAAGGCAAATTGGATAAGCATGGGAAGCCTACTGAGAATACTCCTCCAGAATGGGGCCGGAATTTGGTTTTACCGACAGGTGGAGATTCTGTTATTGCAAGTGCTGCTGCTGAGCTTGCTGCTGCACCTGCTGTTGCCACTGAGACAACTTCTGTTTCAGAGAAGAAGGAGAAGAAAAAACGCAAACAAGAGGAAATAACTGACAGTCCTGTTCCAGTTCCTGCAAAGAAAAGCAAAGGTGAAGAAGTAGCTGAGGTTGAAGTTGAGAAGACAGAAAAGAAGAAGAAAAAGAAAAAGGATAAAGACAATGGTGTTTTGGATTCTTCAGATGGGGAGAAGTCTGTGAAGGTGAAGAAGCACAAAGATAAAGAAGAAGCTGGTTCACCTCAAACTGAAAAGTCTGAAAAGAAGAAAAAGAAGAAAAACAAAGAGGCTGAGGATCATGGTGCTGATGCTGCCACTGATAATGGGAAGGGTGATGGTGAGGCTGATAAGAGTGAGAAAAAGAAGAAGAAGAAGAAAAAGGATAAATCGGATGCTGACGAAGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000154 GO:0000495 GO:0001522 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005732 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009506 GO:0009982 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0016072 GO:0016073 GO:0016074 GO:0016556 GO:0016853 GO:0016866 GO:0022613 GO:0030054 GO:0031118 GO:0031120 GO:0031123 GO:0031126 GO:0031429 GO:0031974 GO:0031981 GO:0032991 GO:0033979 GO:0034470 GO:0034641 GO:0034660 GO:0034964 GO:0040031 GO:0042254 GO:0043144 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043628 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0055044 GO:0070013 GO:0071704 GO:0071840 GO:0072588 GO:0090304 GO:1901360 GO:1902494 GO:1990481 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03008 map03008

Pfam Domains

Protein Families

DKCLD PUA TruB_C_2 TruB_N

Protein Analysis

577

Amino Acids

64.08

Weight (kDa)

9.31

Isoelectric Point (pI)

37.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DKCLD	PF08068	49 - 106	5.1e-31	DKCLD (NUC011) domain
TruB_N	PF01509	110 - 226	9.2e-21	TruB family pseudouridylate synthase (N terminal domain)
TruB_C_2	PF16198	227 - 293	1e-26	tRNA pseudouridylate synthase B C-terminal domain
PUA	PF01472	297 - 370	1.4e-23	PUA domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010475)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G57150
fragaria_vesca	FvH4_5g20900 FvH4_5g20900 FvH4_5g20900
malus_domestica	MD06G1043100.v1.1 MD16G1267700.v1.1 MD16G1267900.v1.1
prunus_persica	Prupe.1G572500_v2.0.a1 Prupe.5G050000_v2.0.a1
pyrus_communis	pycom06g03610 pycom16g23720
rosa_chinensis	RchiOBHm_Chr7g0207911
rosa_laevigata	RLG00000003276
rosa_multiflora	Rmu_sc0006577.1_g000004
rosa_roxburghii	Rroxscaffold_3G00251050
rosa_rugosa	Rorug07G0100200
rosa_samantha	Rh7AG233600 Rh7BG229200 Rh7CG248900 Rh7DG239600
rosa_wichuraiana	Rw0G001290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1318
Acc36I	ACCTGC	1 cut(s)	1318
Acc65I	GGTACC	1 cut(s)	668
AccB1I	GGYRCC	2 cut(s)	668, 935
AccBSI	CCGCTC	1 cut(s)	235
AciI	CCGC	6 cut(s)	181, 233, 241, 291, 363, 626
AclWI	GGATC	2 cut(s)	368, 1629
AcsI	RAATTY	1 cut(s)	1243
AcuI	CTGAAG	1 cut(s)	1491
AdeI	CACNNNGTG	1 cut(s)	689
AfaI	GTAC	3 cut(s)	227, 367, 670
AfiI	CCNNNNNNNGG	4 cut(s)	31, 1235, 1246, 1262
AgsI	TTSAA	2 cut(s)	521, 1442
AjnI	CCWGG	2 cut(s)	138, 952
AjuI	GAANNNNNNNTTGG	2 cut(s)	1127, 1159
AleI	CACNNNNGTG	1 cut(s)	1058
AluBI	AGCT	8 cut(s)	126, 485, 580, 610, 736, 1297, 1433, 1559
AluI	AGCT	8 cut(s)	126, 485, 580, 610, 736, 1297, 1433, 1559
Alw21I	GWGCWC	1 cut(s)	128
Alw26I	GTCTC	2 cut(s)	438, 1319
AlwI	GGATC	2 cut(s)	368, 1629
AlwNI	CAGNNNCTG	1 cut(s)	1403
Ama87I	CYCGRG	2 cut(s)	220, 510
AoxI	GGCC	7 cut(s)	33, 155, 242, 507, 548, 1148, 1237
ApeKI	GCWGC	7 cut(s)	577, 958, 1286, 1289, 1301, 1304, 1637
ApoI	RAATTY	1 cut(s)	1243
Asp718I	GGTACC	1 cut(s)	668
AspA2I	CCTAGG	1 cut(s)	1130
AspS9I	GGNCC	6 cut(s)	97, 156, 508, 591, 1148, 1237
AsuC2I	CCSGG	3 cut(s)	413, 511, 512
AsuHPI	GGTGA	8 cut(s)	776, 1031, 1120, 1433, 1543, 1557, 1670, 1676
AsuII	TTCGAA	1 cut(s)	193
AvaI	CYCGRG	2 cut(s)	220, 510
AvaII	GGWCC	2 cut(s)	97, 591
AvrII	CCTAGG	1 cut(s)	1130
AxyI	CCTNAGG	1 cut(s)	378
BaeGI	GKGCMC	1 cut(s)	517
BaeI	ACNNNNGTAYC	2 cut(s)	660, 693
BanI	GGYRCC	2 cut(s)	668, 935
BanII	GRGCYC	1 cut(s)	128
BbsI	GAAGAC	2 cut(s)	356, 1457
Bbv12I	GWGCWC	1 cut(s)	128
BbvCI	CCTCAGC	3 cut(s)	962, 1434, 1617
BbvI	GCAGC	7 cut(s)	589, 945, 1273, 1276, 1288, 1291, 1624
BccI	CCATC	5 cut(s)	602, 1057, 1105, 1505, 1655
BciT130I	CCWGG	2 cut(s)	140, 954
BciVI	GTATCC	1 cut(s)	1114
BclI	TGATCA	2 cut(s)	111, 1075
BcnI	CCSGG	3 cut(s)	413, 511, 512
BcoDI	GTCTC	2 cut(s)	438, 1319
BfaI	CTAG	1 cut(s)	1131
BfmI	CTRYAG	1 cut(s)	564
BfuAI	ACCTGC	1 cut(s)	1318
BfuI	GTATCC	1 cut(s)	1114
BglI	GCCNNNNNGGC	1 cut(s)	506
BisI	GCNGC	8 cut(s)	242, 578, 959, 1287, 1290, 1302, 1305, 1638
BlnI	CCTAGG	1 cut(s)	1130
BlpI	GCTNAGC	1 cut(s)	1293
BlsI	GCNGC	8 cut(s)	243, 579, 960, 1288, 1291, 1303, 1306, 1639
Bme1390I	CCNGG	5 cut(s)	140, 413, 511, 512, 954
Bme18I	GGWCC	2 cut(s)	97, 591
BmeT110I	CYCGRG	2 cut(s)	220, 510
BmgT120I	GGNCC	6 cut(s)	97, 156, 508, 591, 1148, 1237
BmiI	GGNNCC	4 cut(s)	670, 937, 1149, 1238
BmrFI	CCNGG	5 cut(s)	140, 413, 511, 512, 954
BmrI	ACTGGG	1 cut(s)	20
BmsI	GCATC	7 cut(s)	342, 613, 643, 793, 1166, 1624, 1708
BmuI	ACTGGG	1 cut(s)	20
BpiI	GAAGAC	2 cut(s)	356, 1457
BplI	GAGNNNNNCTC	2 cut(s)	115, 147
BpmI	CTGGAG	1 cut(s)	1212
Bpu10I	CCTNAGC	5 cut(s)	581, 737, 962, 1434, 1617
Bpu1102I	GCTNAGC	1 cut(s)	1293
Bpu14I	TTCGAA	1 cut(s)	193
BpuEI	CTTGAG	4 cut(s)	152, 221, 635, 887
BpuMI	CCSGG	3 cut(s)	413, 511, 512
BsaBI	GATNNNNATC	1 cut(s)	1125
BsaJI	CCNNGG	7 cut(s)	138, 219, 501, 510, 953, 1130, 1151
BsaXI	ACNNNNNCTCC	6 cut(s)	287, 317, 981, 1011, 1257, 1287
Bsc4I	CCNNNNNNNGG	4 cut(s)	31, 1235, 1246, 1262
Bse1I	ACTGG	6 cut(s)	26, 202, 358, 535, 882, 1397
Bse21I	CCTNAGG	1 cut(s)	378
Bse8I	GATNNNNATC	1 cut(s)	1125
BseBI	CCWGG	2 cut(s)	140, 954
BseDI	CCNNGG	7 cut(s)	138, 219, 501, 510, 953, 1130, 1151
BseGI	GGATG	5 cut(s)	333, 808, 838, 1157, 1723
BseJI	GATNNNNATC	1 cut(s)	1125
BseLI	CCNNNNNNNGG	4 cut(s)	31, 1235, 1246, 1262
BseNI	ACTGG	6 cut(s)	26, 202, 358, 535, 882, 1397
BseRI	GAGGAG	2 cut(s)	862, 1215
BseSI	GKGCMC	1 cut(s)	517
BseXI	GCAGC	7 cut(s)	589, 945, 1273, 1276, 1288, 1291, 1624
BsgI	GTGCAG	2 cut(s)	465, 1290
BshFI	GGCC	7 cut(s)	35, 157, 244, 509, 550, 1150, 1239
BshNI	GGYRCC	2 cut(s)	668, 935
BsiHKAI	GWGCWC	1 cut(s)	128
BsiHKCI	CYCGRG	2 cut(s)	220, 510
BsiSI	CCGG	3 cut(s)	412, 511, 1240
BslFI	GGGAC	1 cut(s)	769
BslI	CCNNNNNNNGG	4 cut(s)	31, 1235, 1246, 1262
BsmAI	GTCTC	2 cut(s)	438, 1319
BsmFI	GGGAC	1 cut(s)	769
BsmI	GAATGC	2 cut(s)	478, 925
BsnI	GGCC	7 cut(s)	35, 157, 244, 509, 550, 1150, 1239
BsoBI	CYCGRG	2 cut(s)	220, 510
Bsp119I	TTCGAA	1 cut(s)	193
Bsp1286I	GDGCHC	2 cut(s)	128, 517
Bsp143I	GATC	4 cut(s)	111, 373, 1075, 1621
Bsp1720I	GCTNAGC	1 cut(s)	1293
BspACI	CCGC	6 cut(s)	181, 233, 241, 291, 363, 626
BspANI	GGCC	7 cut(s)	35, 157, 244, 509, 550, 1150, 1239
BspLI	GGNNCC	4 cut(s)	670, 937, 1149, 1238
BspMAI	CTGCAG	1 cut(s)	568
BspMI	ACCTGC	1 cut(s)	1318
BspPI	GGATC	2 cut(s)	368, 1629
BspT104I	TTCGAA	1 cut(s)	193
BspT107I	GGYRCC	2 cut(s)	668, 935
BsrBI	CCGCTC	1 cut(s)	235
BsrI	ACTGG	6 cut(s)	26, 202, 358, 535, 882, 1397
BssECI	CCNNGG	7 cut(s)	138, 219, 501, 510, 953, 1130, 1151
BssMI	GATC	4 cut(s)	111, 373, 1075, 1621
BssT1I	CCWWGG	3 cut(s)	501, 1130, 1151
Bst2UI	CCWGG	2 cut(s)	140, 954
Bst4CI	ACNGT	2 cut(s)	687, 1388
Bst6I	CTCTTC	1 cut(s)	219
BstAPI	GCANNNNNTGC	2 cut(s)	920, 1286
BstBI	TTCGAA	1 cut(s)	193
BstC8I	GCNNGC	5 cut(s)	291, 472, 476, 608, 1299
BstEII	GGTNACC	1 cut(s)	781
BstF5I	GGATG	5 cut(s)	333, 808, 838, 1157, 1723
BstKTI	GATC	4 cut(s)	114, 376, 1078, 1624
BstMAI	GTCTC	2 cut(s)	438, 1319
BstMBI	GATC	4 cut(s)	111, 373, 1075, 1621
BstNI	CCWGG	2 cut(s)	140, 954
BstPI	GGTNACC	1 cut(s)	781
BstSCI	CCNGG	5 cut(s)	138, 411, 509, 510, 952
BstSFI	CTRYAG	1 cut(s)	564
BstSLI	GKGCMC	1 cut(s)	517
BstV1I	GCAGC	7 cut(s)	589, 945, 1273, 1276, 1288, 1291, 1624
BstV2I	GAAGAC	2 cut(s)	356, 1457
Bsu36I	CCTNAGG	1 cut(s)	378
BsuI	GTATCC	1 cut(s)	1114
BsuRI	GGCC	7 cut(s)	35, 157, 244, 509, 550, 1150, 1239
BtsCI	GGATG	5 cut(s)	333, 808, 838, 1157, 1723
BtsI	GCAGTG	2 cut(s)	921, 1053
BtsIMutI	CAGTG	8 cut(s)	48, 158, 528, 683, 921, 1053, 1320, 1641
BveI	ACCTGC	1 cut(s)	1318
Cac8I	GCNNGC	5 cut(s)	291, 472, 476, 608, 1299
CaiI	CAGNNNCTG	1 cut(s)	1403
Cfr13I	GGNCC	6 cut(s)	97, 156, 508, 591, 1148, 1237
Cfr9I	CCCGGG	1 cut(s)	510
Csp6I	GTAC	3 cut(s)	226, 366, 669
CviQI	GTAC	3 cut(s)	226, 366, 669
DpnI	GATC	4 cut(s)	113, 375, 1077, 1623
DpnII	GATC	4 cut(s)	111, 373, 1075, 1621
DraIII	CACNNNGTG	1 cut(s)	689
Eam1104I	CTCTTC	1 cut(s)	219
EarI	CTCTTC	1 cut(s)	219
Ecl136II	GAGCTC	1 cut(s)	126
Eco130I	CCWWGG	3 cut(s)	501, 1130, 1151
Eco24I	GRGCYC	1 cut(s)	128
Eco32I	GATATC	1 cut(s)	561
Eco47I	GGWCC	2 cut(s)	97, 591
Eco53kI	GAGCTC	1 cut(s)	126
Eco57I	CTGAAG	1 cut(s)	1491
Eco81I	CCTNAGG	1 cut(s)	378
Eco88I	CYCGRG	2 cut(s)	220, 510
Eco91I	GGTNACC	1 cut(s)	781
EcoICRI	GAGCTC	1 cut(s)	126
EcoO65I	GGTNACC	1 cut(s)	781
EcoRII	CCWGG	2 cut(s)	138, 952
EcoRV	GATATC	1 cut(s)	561
EcoT14I	CCWWGG	3 cut(s)	501, 1130, 1151
EcoT22I	ATGCAT	1 cut(s)	792
EcoT38I	GRGCYC	1 cut(s)	128
ErhI	CCWWGG	3 cut(s)	501, 1130, 1151
FalI	AAGNNNNNCTT	2 cut(s)	987, 1019
FaqI	GGGAC	1 cut(s)	769
FauI	CCCGC	2 cut(s)	240, 298
FauNDI	CATATG	1 cut(s)	726
FbaI	TGATCA	2 cut(s)	111, 1075
Fnu4HI	GCNGC	8 cut(s)	242, 578, 959, 1287, 1290, 1302, 1305, 1638
FokI	GGATG	5 cut(s)	320, 815, 845, 1144, 1730
FriOI	GRGCYC	1 cut(s)	128
Fsp4HI	GCNGC	8 cut(s)	242, 578, 959, 1287, 1290, 1302, 1305, 1638
FspBI	CTAG	1 cut(s)	1131
GluI	GCNGC	8 cut(s)	242, 578, 959, 1287, 1290, 1302, 1305, 1638
GsuI	CTGGAG	1 cut(s)	1212
HaeIII	GGCC	7 cut(s)	35, 157, 244, 509, 550, 1150, 1239
HapII	CCGG	3 cut(s)	412, 511, 1240
HindIII	AAGCTT	1 cut(s)	608
HinfI	GANTC	6 cut(s)	130, 521, 908, 949, 1268, 1502
HpaII	CCGG	3 cut(s)	412, 511, 1240
HphI	GGTGA	8 cut(s)	776, 1031, 1120, 1433, 1543, 1557, 1670, 1676
Hpy166II	GTNNAC	2 cut(s)	97, 1565
Hpy188I	TCNGA	8 cut(s)	10, 263, 546, 879, 1342, 1510, 1585, 1717
Hpy188III	TCNNGA	7 cut(s)	169, 281, 371, 571, 614, 649, 1229
Hpy8I	GTNNAC	2 cut(s)	97, 1565
Hpy99I	CGWCG	1 cut(s)	17
HpyAV	CCTTC	7 cut(s)	40, 309, 347, 1178, 1342, 1522, 1648
HpyCH4III	ACNGT	2 cut(s)	687, 1388
KpnI	GGTACC	1 cut(s)	672
Ksp22I	TGATCA	2 cut(s)	111, 1075
Kzo9I	GATC	4 cut(s)	111, 373, 1075, 1621
LmnI	GCTCC	1 cut(s)	733
Lsp1109I	GCAGC	7 cut(s)	589, 945, 1273, 1276, 1288, 1291, 1624
LweI	GCATC	7 cut(s)	342, 613, 643, 793, 1166, 1624, 1708
MaeI	CTAG	1 cut(s)	1131
MaeIII	GTNAC	3 cut(s)	356, 382, 781
MalI	GATC	4 cut(s)	113, 375, 1077, 1623
MbiI	CCGCTC	1 cut(s)	235
MboI	GATC	4 cut(s)	111, 373, 1075, 1621
MfeI	CAATTG	1 cut(s)	1026
MhlI	GDGCHC	2 cut(s)	128, 517
MluCI	AATT	6 cut(s)	978, 1026, 1038, 1172, 1190, 1243
MlyI	GAGTC	2 cut(s)	124, 902
Mph1103I	ATGCAT	1 cut(s)	792
MseI	TTAA	2 cut(s)	327, 1098
MslI	CAYNNNNRTG	3 cut(s)	41, 151, 1058
MspA1I	CMGCKG	2 cut(s)	363, 485
MspI	CCGG	3 cut(s)	412, 511, 1240
MspR9I	CCNGG	5 cut(s)	140, 413, 511, 512, 954
MunI	CAATTG	1 cut(s)	1026
Mva1269I	GAATGC	2 cut(s)	478, 925
MvaI	CCWGG	2 cut(s)	140, 954
NciI	CCSGG	3 cut(s)	413, 511, 512
NdeI	CATATG	1 cut(s)	726
NdeII	GATC	4 cut(s)	111, 373, 1075, 1621
NlaIV	GGNNCC	4 cut(s)	670, 937, 1149, 1238
NmuCI	GTSAC	1 cut(s)	356
NsiI	ATGCAT	1 cut(s)	792
NspV	TTCGAA	1 cut(s)	193
OliI	CACNNNNGTG	1 cut(s)	1058
PaqCI	CACCTGC	1 cut(s)	1318
PcsI	WCGNNNNNNNCGW	1 cut(s)	337
PctI	GAATGC	2 cut(s)	478, 925
PfeI	GAWTC	4 cut(s)	521, 949, 1268, 1502
PkrI	GCNGC	8 cut(s)	243, 579, 960, 1288, 1291, 1303, 1306, 1639
PleI	GAGTC	2 cut(s)	124, 902
PpsI	GAGTC	2 cut(s)	124, 902
Psp124BI	GAGCTC	1 cut(s)	128
Psp6I	CCWGG	2 cut(s)	138, 952
PspEI	GGTNACC	1 cut(s)	781
PspGI	CCWGG	2 cut(s)	138, 952
PspN4I	GGNNCC	4 cut(s)	670, 937, 1149, 1238
PspPI	GGNCC	6 cut(s)	97, 156, 508, 591, 1148, 1237
PstI	CTGCAG	1 cut(s)	568
PstNI	CAGNNNCTG	1 cut(s)	1403
PvuII	CAGCTG	1 cut(s)	485
RsaI	GTAC	3 cut(s)	227, 367, 670
RsaNI	GTAC	3 cut(s)	226, 366, 669
RseI	CAYNNNNRTG	3 cut(s)	41, 151, 1058
SacI	GAGCTC	1 cut(s)	128
SaqAI	TTAA	2 cut(s)	327, 1098
SatI	GCNGC	8 cut(s)	242, 578, 959, 1287, 1290, 1302, 1305, 1638
Sau3AI	GATC	4 cut(s)	111, 373, 1075, 1621
Sau96I	GGNCC	6 cut(s)	97, 156, 508, 591, 1148, 1237
SchI	GAGTC	2 cut(s)	124, 902
ScrFI	CCNGG	5 cut(s)	140, 413, 511, 512, 954
SduI	GDGCHC	2 cut(s)	128, 517
SfaNI	GCATC	7 cut(s)	342, 613, 643, 793, 1166, 1624, 1708
SfcI	CTRYAG	1 cut(s)	564
SfuI	TTCGAA	1 cut(s)	193
SinI	GGWCC	2 cut(s)	97, 591
SmaI	CCCGGG	1 cut(s)	512
SmiMI	CAYNNNNRTG	3 cut(s)	41, 151, 1058
SmlI	CTYRAG	4 cut(s)	167, 236, 614, 866
SmoI	CTYRAG	4 cut(s)	167, 236, 614, 866
SrfI	GCCCGGGC	1 cut(s)	512
Sse9I	AATT	6 cut(s)	978, 1026, 1038, 1172, 1190, 1243
SsiI	CCGC	6 cut(s)	181, 233, 241, 291, 363, 626
SspMI	CTAG	1 cut(s)	1131
SstI	GAGCTC	1 cut(s)	128
StyD4I	CCNGG	5 cut(s)	138, 411, 509, 510, 952
StyI	CCWWGG	3 cut(s)	501, 1130, 1151
TaaI	ACNGT	2 cut(s)	687, 1388
TaqI	TCGA	2 cut(s)	128, 193
TasI	AATT	6 cut(s)	978, 1026, 1038, 1172, 1190, 1243
TauI	GCSGC	1 cut(s)	244
TfiI	GAWTC	4 cut(s)	521, 949, 1268, 1502
Tru1I	TTAA	2 cut(s)	327, 1098
Tru9I	TTAA	2 cut(s)	327, 1098
TscAI	CASTG	8 cut(s)	48, 158, 535, 690, 921, 1060, 1327, 1648
TseFI	GTSAC	1 cut(s)	356
TseI	GCWGC	7 cut(s)	577, 958, 1286, 1289, 1301, 1304, 1637
Tsp45I	GTSAC	1 cut(s)	356
TspDTI	ATGAA	3 cut(s)	615, 990, 1178
TspMI	CCCGGG	1 cut(s)	510
TspRI	CASTG	8 cut(s)	48, 158, 535, 690, 921, 1060, 1327, 1648
VpaK11BI	GGWCC	2 cut(s)	97, 591
XapI	RAATTY	1 cut(s)	1243
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1060
XmaI	CCCGGG	1 cut(s)	510
XmaJI	CCTAGG	1 cut(s)	1130
XspI	CTAG	1 cut(s)	1131
Zsp2I	ATGCAT	1 cut(s)	792

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.