Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0010475

Physical Location & Seq

Genomic Coordinates Reverse (-)

34373556 .. 34376277

Gene Structure 2722 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1267700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1560 bp

ATGATCAAGCCCCAGAGCTCAACTCCCTCCTTGGACACCTCTCAGTGGCCTATCCTTCTCAAAAACTATGACCGACTCAATGTTCGAACTGGACACTACACCCCTCTACCCTCAGGGTTCTCCCCCCTTAAGAGGCCTCTTGCTGAGTACATCCGATATGGTGTTCTCAATCTTGACAAGCCTGCAAACCCCTCTTCTCACGAGGTTGTTGCTTGGATTAAGCGCATCCTTCGCGTTGAGAAAACTGGCCACAGCGGTACTCTGGATCCTAAGCAGGGTGCAGGGAAAGAGTATGTTTGTGTTGCCCGCTTGCATTCAGCTGTGCCTGATGTTGCCAAAGTGGCTCAGGCCCTTGAATCTCTTACTGGGGCTGTCTTTCAGAGGCCACCTTTGATATCAGCAGTCAAGAGGCAGCTTAGGATTAGGACCATCTATGAAAGCAAACTTCTTGAGTTTGATGCAGATAAACATTTGGTCGTTTTCTGGATTTCTTGCGAAGCAGGAACCTATGTGCGGACGCTCTGTGTGCATTTGGGTCTGCTGCTTGGTGTGGGAGGTCATATGCAGGAGCTTAGGAGGGTCAGGTCGGGGATTATGGGCGAGAAAGACAACATGGTCACAATGCATGACGTCATGGATGCTCAGTGGGCTTATGACACTAATAGGGATGAGAGTTATTTGAGGAGGGTCATCATGCCTCTTGAGGTTCTCTTGACCAGTTATAAGAGATTGGTTGTCAAGGACTCAGCAGTGAATGCCATTTGCTACGGTGCCAAGTTAATGATTCCAGGGCTGCTCAGGTTTGAGAATGATATCGAGGTTGGAGAGGAAGTTGTGCTGATGACTACCAAAGGAGAAGCCATTGCATTGGGAATTGCTGAGATGACCACTGCTGTGATGGCAACTTGTGATCATGGTGTGGTGGCAAAGATTAAGAGGGTGGTAATGGATCGGGATACTTACCCAAGGAAGTGGGGATTGGGACCAAGGGCGTCCATGAAGAAGAAATTGATTGCCGAAGGGAAATTGGATAAGCATGGGAAGCCCACTGAGAATACTCCGCAAGAATGGATGAGAAATGTGGTTTTGTCTGCAGGTGGGGATTCTATTGTTGCAAGCACTGCTGCTGCGCTTGACACTGAACCTGCTGTGGCAGAGAAGGAGACCGCTCTCGTAGACAAGGAGAAGAAGGAGAAGAAGAAGAAAAAGGACAAACATAAGGATGAAGAGGAAATTAAGGAGGGGCGTAAACGCAAACTAGAGGAACCCACAGAGAGCACTGTTCCGGTTCCTGCAAAGAAAAGCAAAGGTGAAGAAGTAGAGCCTGAAGTTGAGAAGTCCGAAAAGAAAAAGAAGAAAAAGGACAAAGAGAATGGCACTGTGGATAATGTTGAAGCTGGTTCACCTGAAACAGAAAAGTCAGAGAAGAAGAAGAAGAAGAAGAAAAACAAAGACGCTGAGGACAATGGTGCTGTGGCTTCTGCCGGCGCTGGTGATGGCGAGGCTGATAAGAGTGAGAAAAAGAAGAAACAGAAGAGTAAAGCAGATGCAGAAGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000154 GO:0000495 GO:0001522 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005732 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009506 GO:0009982 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0016072 GO:0016073 GO:0016074 GO:0016556 GO:0016853 GO:0016866 GO:0022613 GO:0030054 GO:0031118 GO:0031120 GO:0031123 GO:0031126 GO:0031429 GO:0031974 GO:0031981 GO:0032991 GO:0033979 GO:0034470 GO:0034641 GO:0034660 GO:0034964 GO:0040031 GO:0042254 GO:0043144 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043628 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0055044 GO:0070013 GO:0071704 GO:0071840 GO:0072588 GO:0090304 GO:1901360 GO:1902494 GO:1990481 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03008 map03008

Pfam Domains

Protein Families

DKCLD PUA TruB_C_2 TruB_N

Protein Analysis

520

Amino Acids

57.61

Weight (kDa)

9.2

Isoelectric Point (pI)

35.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DKCLD	PF08068	13 - 70	3e-31	DKCLD (NUC011) domain
TruB_C_2	PF16198	172 - 238	8.6e-27	tRNA pseudouridylate synthase B C-terminal domain
PUA	PF01472	242 - 315	8.7e-24	PUA domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010475)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G57150
fragaria_vesca	FvH4_5g20900 FvH4_5g20900 FvH4_5g20900
malus_domestica	MD06G1043100.v1.1 MD16G1267700.v1.1 MD16G1267900.v1.1
prunus_persica	Prupe.1G572500_v2.0.a1 Prupe.5G050000_v2.0.a1
pyrus_communis	pycom06g03610 pycom16g23720
rosa_chinensis	RchiOBHm_Chr7g0207911
rosa_laevigata	RLG00000003276
rosa_multiflora	Rmu_sc0006577.1_g000004
rosa_roxburghii	Rroxscaffold_3G00251050
rosa_rugosa	Rorug07G0100200
rosa_samantha	Rh7AG233600 Rh7BG229200 Rh7CG248900 Rh7DG239600
rosa_wichuraiana	Rw0G001290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	723
AarI	CACCTGC	1 cut(s)	1085
AasI	GACNNNNNNGTC	1 cut(s)	614
AatII	GACGTC	1 cut(s)	633
Acc36I	ACCTGC	2 cut(s)	1085, 1153
AccB1I	GGYRCC	1 cut(s)	770
AccBSI	CCGCTC	1 cut(s)	1169
AccI	GTMKAC	1 cut(s)	1176
AccII	CGCG	1 cut(s)	234
AciI	CCGC	5 cut(s)	255, 307, 514, 1061, 1167
AclWI	GGATC	3 cut(s)	260, 273, 957
AcoI	YGGCCR	1 cut(s)	247
AcuI	CTGAAG	1 cut(s)	1347
AcyI	GRCGYC	2 cut(s)	630, 992
AfaI	GTAC	2 cut(s)	149, 259
AfiI	CCNNNNNNNGG	4 cut(s)	45, 132, 275, 513
AflII	CTTAAG	1 cut(s)	128
AgsI	TTSAA	2 cut(s)	356, 1394
AjnI	CCWGG	1 cut(s)	787
AjuI	GAANNNNNNNTTGG	2 cut(s)	962, 994
AleI	CACNNNNGTG	1 cut(s)	893
AluBI	AGCT	5 cut(s)	18, 320, 415, 571, 1397
AluI	AGCT	5 cut(s)	18, 320, 415, 571, 1397
Alw21I	GWGCWC	2 cut(s)	20, 1280
Alw26I	GTCTC	1 cut(s)	1157
AlwI	GGATC	3 cut(s)	260, 273, 957
AoxI	GGCC	5 cut(s)	47, 134, 247, 348, 383
ApeKI	GCWGC	5 cut(s)	412, 541, 793, 1124, 1127
ArsI	GACNNNNNNTTYG	2 cut(s)	66, 98
AspLEI	GCGC	3 cut(s)	225, 1132, 1490
AspS9I	GGNCC	3 cut(s)	349, 426, 983
AsuHPI	GGTGA	3 cut(s)	1322, 1395, 1505
AsuII	TTCGAA	1 cut(s)	85
AvaII	GGWCC	2 cut(s)	426, 983
AxyI	CCTNAGG	1 cut(s)	112
BalI	TGGCCA	1 cut(s)	249
BamHI	GGATCC	1 cut(s)	265
BanI	GGYRCC	1 cut(s)	770
BanII	GRGCYC	1 cut(s)	20
BauI	CACGAG	1 cut(s)	200
Bbv12I	GWGCWC	2 cut(s)	20, 1280
BbvCI	CCTCAGC	1 cut(s)	1458
BbvI	GCAGC	5 cut(s)	424, 528, 780, 1111, 1114
BccI	CCATC	3 cut(s)	437, 892, 1490
BcgI	CGANNNNNNTGC	2 cut(s)	191, 225
BciT130I	CCWGG	1 cut(s)	789
BciVI	GTATCC	1 cut(s)	949
BclI	TGATCA	2 cut(s)	3, 910
BcoDI	GTCTC	1 cut(s)	1157
BfaI	CTAG	1 cut(s)	1259
BfmI	CTRYAG	1 cut(s)	1092
BfoI	RGCGCY	1 cut(s)	1491
BfrI	CTTAAG	1 cut(s)	128
BfuAI	ACCTGC	2 cut(s)	1085, 1153
BfuI	GTATCC	1 cut(s)	949
BglI	GCCNNNNNGGC	1 cut(s)	341
BisI	GCNGC	5 cut(s)	413, 542, 794, 1125, 1128
BlsI	GCNGC	5 cut(s)	414, 543, 795, 1126, 1129
Bme1390I	CCNGG	1 cut(s)	789
Bme18I	GGWCC	2 cut(s)	426, 983
BmgT120I	GGNCC	3 cut(s)	349, 426, 983
BmiI	GGNNCC	6 cut(s)	267, 505, 772, 984, 1266, 1290
BmrFI	CCNGG	1 cut(s)	789
BmrI	ACTGGG	1 cut(s)	375
BmsI	GCATC	4 cut(s)	234, 448, 628, 1537
BmuI	ACTGGG	1 cut(s)	375
BplI	GAGNNNNNCTC	2 cut(s)	7, 39
Bpu10I	CCTNAGC	6 cut(s)	270, 345, 416, 572, 797, 1458
Bpu14I	TTCGAA	1 cut(s)	85
BpuEI	CTTGAG	2 cut(s)	470, 722
BsaHI	GRCGYC	2 cut(s)	630, 992
BsaI	GGTCTC	1 cut(s)	1157
BsaJI	CCNNGG	4 cut(s)	30, 788, 965, 986
BsaWI	WCCGGW	1 cut(s)	1285
BsaXI	ACNNNNNCTCC	2 cut(s)	816, 846
Bsc4I	CCNNNNNNNGG	4 cut(s)	45, 132, 275, 513
Bse118I	RCCGGY	1 cut(s)	1484
Bse1I	ACTGG	4 cut(s)	94, 250, 370, 717
Bse21I	CCTNAGG	1 cut(s)	112
Bse3DI	GCAATG	1 cut(s)	861
BseBI	CCWGG	1 cut(s)	789
BseDI	CCNNGG	4 cut(s)	30, 788, 965, 986
BseGI	GGATG	6 cut(s)	150, 225, 643, 673, 1077, 1228
BseLI	CCNNNNNNNGG	4 cut(s)	45, 132, 275, 513
BseMI	GCAATG	1 cut(s)	861
BseNI	ACTGG	4 cut(s)	94, 250, 370, 717
BseRI	GAGGAG	1 cut(s)	697
BseXI	GCAGC	5 cut(s)	424, 528, 780, 1111, 1114
BsgI	GTGCAG	1 cut(s)	300
Bsh1236I	CGCG	1 cut(s)	234
BshFI	GGCC	5 cut(s)	49, 136, 249, 350, 385
BshNI	GGYRCC	1 cut(s)	770
BsiHKAI	GWGCWC	2 cut(s)	20, 1280
BsiSI	CCGG	2 cut(s)	1286, 1485
BslFI	GGGAC	1 cut(s)	996
BslI	CCNNNNNNNGG	4 cut(s)	45, 132, 275, 513
BsmAI	GTCTC	1 cut(s)	1157
BsmFI	GGGAC	1 cut(s)	996
BsmI	GAATGC	2 cut(s)	313, 760
BsnI	GGCC	5 cut(s)	49, 136, 249, 350, 385
Bso31I	GGTCTC	1 cut(s)	1157
Bsp119I	TTCGAA	1 cut(s)	85
Bsp1286I	GDGCHC	2 cut(s)	20, 1280
Bsp143I	GATC	4 cut(s)	3, 265, 910, 949
BspACI	CCGC	5 cut(s)	255, 307, 514, 1061, 1167
BspANI	GGCC	5 cut(s)	49, 136, 249, 350, 385
BspFNI	CGCG	1 cut(s)	234
BspLI	GGNNCC	6 cut(s)	267, 505, 772, 984, 1266, 1290
BspMAI	CTGCAG	1 cut(s)	1096
BspMI	ACCTGC	2 cut(s)	1085, 1153
BspPI	GGATC	3 cut(s)	260, 273, 957
BspT104I	TTCGAA	1 cut(s)	85
BspT107I	GGYRCC	1 cut(s)	770
BspTI	CTTAAG	1 cut(s)	128
BspTNI	GGTCTC	1 cut(s)	1157
BsrBI	CCGCTC	1 cut(s)	1169
BsrDI	GCAATG	1 cut(s)	861
BsrFI	RCCGGY	1 cut(s)	1484
BsrI	ACTGG	4 cut(s)	94, 250, 370, 717
BssAI	RCCGGY	1 cut(s)	1484
BssECI	CCNNGG	4 cut(s)	30, 788, 965, 986
BssMI	GATC	4 cut(s)	3, 265, 910, 949
BssNI	GRCGYC	2 cut(s)	630, 992
BssSI	CACGAG	1 cut(s)	200
BssT1I	CCWWGG	3 cut(s)	30, 965, 986
Bst2BI	CACGAG	1 cut(s)	200
Bst2UI	CCWGG	1 cut(s)	789
Bst4CI	ACNGT	3 cut(s)	770, 1282, 1381
Bst6I	CTCTTC	3 cut(s)	199, 1221, 1529
BstACI	GRCGYC	2 cut(s)	630, 992
BstAFI	CTTAAG	1 cut(s)	128
BstAPI	GCANNNNNTGC	2 cut(s)	755, 1121
BstBI	TTCGAA	1 cut(s)	85
BstC8I	GCNNGC	5 cut(s)	183, 307, 311, 1117, 1486
BstF5I	GGATG	6 cut(s)	150, 225, 643, 673, 1077, 1228
BstFNI	CGCG	1 cut(s)	234
BstH2I	RGCGCY	1 cut(s)	1491
BstHHI	GCGC	3 cut(s)	225, 1132, 1490
BstKTI	GATC	4 cut(s)	6, 268, 913, 952
BstMAI	GTCTC	1 cut(s)	1157
BstMBI	GATC	4 cut(s)	3, 265, 910, 949
BstMWI	GCNNNNNNNGC	8 cut(s)	231, 341, 526, 647, 755, 899, 1042, 1121
BstNI	CCWGG	1 cut(s)	789
BstSCI	CCNGG	1 cut(s)	787
BstSFI	CTRYAG	1 cut(s)	1092
BstUI	CGCG	1 cut(s)	234
BstV1I	GCAGC	5 cut(s)	424, 528, 780, 1111, 1114
BstX2I	RGATCY	1 cut(s)	265
BstXI	CCANNNNNNTGG	2 cut(s)	868, 972
BstYI	RGATCY	1 cut(s)	265
Bsu36I	CCTNAGG	1 cut(s)	112
BsuI	GTATCC	1 cut(s)	949
BsuRI	GGCC	5 cut(s)	49, 136, 249, 350, 385
BtsCI	GGATG	6 cut(s)	150, 225, 643, 673, 1077, 1228
BtsI	GCAGTG	3 cut(s)	756, 888, 1119
BtsIMutI	CAGTG	9 cut(s)	50, 650, 756, 888, 1047, 1119, 1137, 1278, 1377
BveI	ACCTGC	2 cut(s)	1085, 1153
Cac8I	GCNNGC	5 cut(s)	183, 307, 311, 1117, 1486
CfoI	GCGC	3 cut(s)	225, 1132, 1490
Cfr10I	RCCGGY	1 cut(s)	1484
Cfr13I	GGNCC	3 cut(s)	349, 426, 983
CseI	GACGC	3 cut(s)	526, 981, 1463
Csp6I	GTAC	2 cut(s)	148, 258
CviAII	CATG	7 cut(s)	613, 626, 634, 694, 914, 997, 1037
CviQI	GTAC	2 cut(s)	148, 258
DpnI	GATC	4 cut(s)	5, 267, 912, 951
DpnII	GATC	4 cut(s)	3, 265, 910, 949
DrdI	GACNNNNNNGTC	1 cut(s)	614
DseDI	GACNNNNNNGTC	1 cut(s)	614
EaeI	YGGCCR	1 cut(s)	247
Eam1104I	CTCTTC	3 cut(s)	199, 1221, 1529
EarI	CTCTTC	3 cut(s)	199, 1221, 1529
Ecl136II	GAGCTC	1 cut(s)	18
Eco130I	CCWWGG	3 cut(s)	30, 965, 986
Eco147I	AGGCCT	1 cut(s)	136
Eco24I	GRGCYC	1 cut(s)	20
Eco31I	GGTCTC	1 cut(s)	1157
Eco32I	GATATC	2 cut(s)	396, 814
Eco47I	GGWCC	2 cut(s)	426, 983
Eco53kI	GAGCTC	1 cut(s)	18
Eco57I	CTGAAG	1 cut(s)	1347
Eco81I	CCTNAGG	1 cut(s)	112
EcoICRI	GAGCTC	1 cut(s)	18
EcoO109I	RGGNCCY	1 cut(s)	349
EcoRII	CCWGG	1 cut(s)	787
EcoRV	GATATC	2 cut(s)	396, 814
EcoT14I	CCWWGG	3 cut(s)	30, 965, 986
EcoT22I	ATGCAT	1 cut(s)	627
EcoT38I	GRGCYC	1 cut(s)	20
ErhI	CCWWGG	3 cut(s)	30, 965, 986
FaeI	CATG	7 cut(s)	616, 629, 637, 697, 917, 1000, 1040
FaqI	GGGAC	1 cut(s)	996
FatI	CATG	7 cut(s)	612, 625, 633, 693, 913, 996, 1036
FauI	CCCGC	1 cut(s)	314
FauNDI	CATATG	1 cut(s)	561
FbaI	TGATCA	2 cut(s)	3, 910
FblI	GTMKAC	1 cut(s)	1176
Fnu4HI	GCNGC	5 cut(s)	413, 542, 794, 1125, 1128
FokI	GGATG	6 cut(s)	137, 212, 650, 680, 1084, 1235
FriOI	GRGCYC	1 cut(s)	20
Fsp4HI	GCNGC	5 cut(s)	413, 542, 794, 1125, 1128
FspBI	CTAG	1 cut(s)	1259
GlaI	GCGC	3 cut(s)	224, 1131, 1489
GluI	GCNGC	5 cut(s)	413, 542, 794, 1125, 1128
HaeII	RGCGCY	1 cut(s)	1491
HaeIII	GGCC	5 cut(s)	49, 136, 249, 350, 385
HapII	CCGG	2 cut(s)	1286, 1485
HgaI	GACGC	3 cut(s)	526, 981, 1463
HhaI	GCGC	3 cut(s)	225, 1132, 1490
Hin1I	GRCGYC	2 cut(s)	630, 992
Hin1II	CATG	7 cut(s)	616, 629, 637, 697, 917, 1000, 1040
Hin6I	GCGC	3 cut(s)	223, 1130, 1488
HinP1I	GCGC	3 cut(s)	223, 1130, 1488
HinfI	GANTC	5 cut(s)	75, 356, 743, 784, 1103
HpaII	CCGG	2 cut(s)	1286, 1485
HphI	GGTGA	3 cut(s)	1322, 1395, 1505
Hpy166II	GTNNAC	3 cut(s)	1177, 1250, 1403
Hpy188I	TCNGA	4 cut(s)	155, 381, 1342, 1423
Hpy188III	TCNNGA	9 cut(s)	173, 200, 263, 406, 449, 484, 701, 712, 953
Hpy8I	GTNNAC	3 cut(s)	1177, 1250, 1403
HpyAV	CCTTC	5 cut(s)	65, 239, 1013, 1153, 1183
HpyCH4III	ACNGT	3 cut(s)	770, 1282, 1381
HpyCH4IV	ACGT	1 cut(s)	630
HpyF10VI	GCNNNNNNNGC	8 cut(s)	231, 341, 526, 647, 755, 899, 1042, 1121
HpySE526I	ACGT	1 cut(s)	630
Hsp92I	GRCGYC	2 cut(s)	630, 992
Hsp92II	CATG	7 cut(s)	616, 629, 637, 697, 917, 1000, 1040
HspAI	GCGC	3 cut(s)	223, 1130, 1488
KroI	GCCGGC	1 cut(s)	1484
KroNI	GCCGGC	1 cut(s)	1486
Ksp22I	TGATCA	2 cut(s)	3, 910
Kzo9I	GATC	4 cut(s)	3, 265, 910, 949
LmnI	GCTCC	1 cut(s)	568
Lsp1109I	GCAGC	5 cut(s)	424, 528, 780, 1111, 1114
LweI	GCATC	4 cut(s)	234, 448, 628, 1537
MaeI	CTAG	1 cut(s)	1259
MaeII	ACGT	1 cut(s)	630
MaeIII	GTNAC	1 cut(s)	616
MalI	GATC	4 cut(s)	5, 267, 912, 951
MbiI	CCGCTC	1 cut(s)	1169
MboI	GATC	4 cut(s)	3, 265, 910, 949
MflI	RGATCY	1 cut(s)	265
MhlI	GDGCHC	2 cut(s)	20, 1280
MlsI	TGGCCA	1 cut(s)	249
MluCI	AATT	4 cut(s)	873, 1007, 1025, 1233
MluNI	TGGCCA	1 cut(s)	249
MlyI	GAGTC	2 cut(s)	69, 737
MmeI	TCCRAC	1 cut(s)	802
Mox20I	TGGCCA	1 cut(s)	249
Mph1103I	ATGCAT	1 cut(s)	627
MroNI	GCCGGC	1 cut(s)	1484
MscI	TGGCCA	1 cut(s)	249
MseI	TTAA	5 cut(s)	129, 219, 779, 933, 1236
MslI	CAYNNNNRTG	2 cut(s)	893, 1221
Msp20I	TGGCCA	1 cut(s)	249
MspA1I	CMGCKG	2 cut(s)	255, 320
MspCI	CTTAAG	1 cut(s)	128
MspI	CCGG	2 cut(s)	1286, 1485
MspR9I	CCNGG	1 cut(s)	789
Mva1269I	GAATGC	2 cut(s)	313, 760
MvaI	CCWGG	1 cut(s)	789
MvnI	CGCG	1 cut(s)	234
MwoI	GCNNNNNNNGC	8 cut(s)	231, 341, 526, 647, 755, 899, 1042, 1121
NaeI	GCCGGC	1 cut(s)	1486
NdeI	CATATG	1 cut(s)	561
NdeII	GATC	4 cut(s)	3, 265, 910, 949
NgoMIV	GCCGGC	1 cut(s)	1484
NlaIII	CATG	7 cut(s)	616, 629, 637, 697, 917, 1000, 1040
NlaIV	GGNNCC	6 cut(s)	267, 505, 772, 984, 1266, 1290
NmuCI	GTSAC	1 cut(s)	616
NsiI	ATGCAT	1 cut(s)	627
NspV	TTCGAA	1 cut(s)	85
OliI	CACNNNNGTG	1 cut(s)	893
PaqCI	CACCTGC	1 cut(s)	1085
PceI	AGGCCT	1 cut(s)	136
PctI	GAATGC	2 cut(s)	313, 760
PdiI	GCCGGC	1 cut(s)	1486
PfeI	GAWTC	3 cut(s)	356, 784, 1103
PkrI	GCNGC	5 cut(s)	414, 543, 795, 1126, 1129
PleI	GAGTC	2 cut(s)	69, 737
PpsI	GAGTC	2 cut(s)	69, 737
PsiI	TTATAA	1 cut(s)	723
Psp124BI	GAGCTC	1 cut(s)	20
Psp6I	CCWGG	1 cut(s)	787
PspGI	CCWGG	1 cut(s)	787
PspN4I	GGNNCC	6 cut(s)	267, 505, 772, 984, 1266, 1290
PspPI	GGNCC	3 cut(s)	349, 426, 983
PstI	CTGCAG	1 cut(s)	1096
PsuI	RGATCY	1 cut(s)	265
PvuII	CAGCTG	1 cut(s)	320
RsaI	GTAC	2 cut(s)	149, 259
RsaNI	GTAC	2 cut(s)	148, 258
RseI	CAYNNNNRTG	2 cut(s)	893, 1221
SacI	GAGCTC	1 cut(s)	20
SaqAI	TTAA	5 cut(s)	129, 219, 779, 933, 1236
SatI	GCNGC	5 cut(s)	413, 542, 794, 1125, 1128
Sau3AI	GATC	4 cut(s)	3, 265, 910, 949
Sau96I	GGNCC	3 cut(s)	349, 426, 983
SchI	GAGTC	2 cut(s)	69, 737
ScrFI	CCNGG	1 cut(s)	789
SduI	GDGCHC	2 cut(s)	20, 1280
SfaNI	GCATC	4 cut(s)	234, 448, 628, 1537
SfcI	CTRYAG	1 cut(s)	1092
SfuI	TTCGAA	1 cut(s)	85
SinI	GGWCC	2 cut(s)	426, 983
SmiMI	CAYNNNNRTG	2 cut(s)	893, 1221
SmlI	CTYRAG	3 cut(s)	128, 449, 701
SmoI	CTYRAG	3 cut(s)	128, 449, 701
Sse9I	AATT	4 cut(s)	873, 1007, 1025, 1233
SseBI	AGGCCT	1 cut(s)	136
SsiI	CCGC	5 cut(s)	255, 307, 514, 1061, 1167
SspMI	CTAG	1 cut(s)	1259
SstI	GAGCTC	1 cut(s)	20
StuI	AGGCCT	1 cut(s)	136
StyD4I	CCNGG	1 cut(s)	787
StyI	CCWWGG	3 cut(s)	30, 965, 986
TaaI	ACNGT	3 cut(s)	770, 1282, 1381
TaiI	ACGT	1 cut(s)	633
TaqI	TCGA	2 cut(s)	85, 816
TaqII	GACCGA	1 cut(s)	87
TasI	AATT	4 cut(s)	873, 1007, 1025, 1233
TatI	WGTACW	1 cut(s)	147
TfiI	GAWTC	3 cut(s)	356, 784, 1103
Tru1I	TTAA	5 cut(s)	129, 219, 779, 933, 1236
Tru9I	TTAA	5 cut(s)	129, 219, 779, 933, 1236
TscAI	CASTG	9 cut(s)	50, 650, 756, 895, 1054, 1126, 1144, 1285, 1384
TseFI	GTSAC	1 cut(s)	616
TseI	GCWGC	5 cut(s)	412, 541, 793, 1124, 1127
Tsp45I	GTSAC	1 cut(s)	616
TspDTI	ATGAA	3 cut(s)	450, 1013, 1239
TspRI	CASTG	9 cut(s)	50, 650, 756, 895, 1054, 1126, 1144, 1285, 1384
Vha464I	CTTAAG	1 cut(s)	128
VpaK11BI	GGWCC	2 cut(s)	426, 983
XcmI	CCANNNNNNNNNTGG	1 cut(s)	895
XmiI	GTMKAC	1 cut(s)	1176
XspI	CTAG	1 cut(s)	1259
ZraI	GACGTC	1 cut(s)	631
Zsp2I	ATGCAT	1 cut(s)	627

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.