FvH4_5g23110

domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0011765
Physical Location & Seq
Forward (+)
14462224 .. 14468294
6071 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g23110.t1

Sequence Viewer

Length: 1257 bp
ATGGGGTGCATGTGCTCTACCTTGGCTTCCAAATTCGCCTTCTTCCCTCCATCTCCCCCAACCTACAAGATCCTCCACCCTGAAAACAACGACGGCAAGCTCACCGTCGTAGCCTCCCCCTCCTCCACCACCACCTCATTCCCTCTGCCTCACGCCCAGGACAACTCCCTCGACGTTTTGCTCATTGACAACAAACGTGGCTCCAAGATTGTCGCCTTCTATCTTCGAAACCCATATGCTCGTCTCACTCTGCTTTACTCTCATGGCAATGCTGCTGACCTTGGTCAACTCTATGATCTCTTTGTCCAGCTCAAGGTCGCTCTCAGGGTCAATCTCATTGGATATGACTATTCTGGCTATGGATCCTCTACTGGCAAGCCAAGTGAGGAAAACACATATGCTGACATAGAGGCAGTATATGAGTGCCTTGAGACTGAATATGGAGTTAGCCAGGAAGATTTGATCTTGTATGGGCAGTCAGTTGGAAGTGGACCAACACTCCACTTGGCATCTAAACTACCAAGATTAAGGGGTGTAGTTCTGCACAGTGCAATTCTCTCTGGCCTTCGTGTTGTCTGCCATGTTAAATTTACATTCTGCTTTGACATTTATAAGAACATCAATAAAATCAAGAACGTGAAGTGCCCTGTGCTAGTGATACATGGTACAGAAGATGATATTGTGAATTGGTTACATGGCAATGGACTGTGGGAAATGGCAAGGGATCCGTATGAGCCTCTATGGATTAAAGGAGGTGGGCACTGCAACTTGGAGCTTTACCCTGATTACATCCGCCATCTTTGCAGATTTGTCCAAGAAATGGAGAACATAACCACTGAAATCCGTCTAAAAAAGATCCGGCAGAACATGCATGTGCACAAGAGATCATGTGCAAGCCATTGCTGCAGAATTAAAGTCTGCCGACCTAAATGCCCTGAATGTTCTATGCCAAAATGTGTAAAATGCTCCTGGCGCCCAAAATGCTCCTCATGCTGCTGGAAGCCGAAATGCTCCTCTTGCTGGAAGCCGAAATGCTCCTCTTGCTGGAAGCCGAAATGCTCCTCATGCTGGAAGCCGAAATGCTCCTCTTGCTGGAAGCCGAAATGCTCCTCTTGCTGGAAGCCAAGCTGCATAAAATGTTGCTGGCGGCCCAAATCCCTGAAATGTGCAGGACCAAGCTGCTGCATTACATGTTTTCAGTGGCGGTGCTGTGTAGGAGGAACACAAAGTGGGTTAGACAGGAGGCAGGATGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DLH Hydrolase_4

Protein Analysis

419

Amino Acids

47.09

Weight (kDa)

8.98

Isoelectric Point (pI)

59.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 82 - 186 7.9e-06 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 84 - 184 1.8e-06 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011765)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32190 AT1G32190
fragaria_vesca FvH4_5g23110 FvH4_5g23110
malus_domestica MD06G1038600.v1.1 MD16G1270400.v1.1
prunus_persica Prupe.5G027400_v2.0.a1 Prupe.5G027400_v2.0.a1
pyrus_communis pycom06g03240
rosa_chinensis RchiOBHm_Chr7g0211991
rosa_laevigata RLG00000002947
rosa_multiflora Rmu_sc0004869.1_g000006
rosa_roxburghii Rroxscaffold_3G00247220
rosa_rugosa Rorug07G0129900
rosa_samantha Rh7AG262700 Rh7BG256800 Rh7CG279300 Rh7DG269500
rosa_wichuraiana Rw7G022420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 612
AccB1I GGYRCC 1 cut(s) 972
AccB7I CCANNNNNTGG 1 cut(s) 820
AciI CCGC 3 cut(s) 793, 1147, 1204
AclWI GGATC 6 cut(s) 64, 357, 370, 719, 732, 850
AcsI RAATTY 2 cut(s) 32, 587
AcyI GRCGYC 1 cut(s) 973
AdeI CACNNNGTG 1 cut(s) 1229
AfaI GTAC 1 cut(s) 667
AfiI CCNNNNNNNGG 7 cut(s) 313, 820, 1020, 1044, 1068, 1092, 1116
AflIII ACRYGT 1 cut(s) 1190
AjnI CCWGG 3 cut(s) 156, 450, 968
AjuI GAANNNNNNNTTGG 2 cut(s) 23, 55
AluBI AGCT 5 cut(s) 100, 310, 775, 1128, 1179
AluI AGCT 5 cut(s) 100, 310, 775, 1128, 1179
Alw21I GWGCWC 2 cut(s) 17, 879
Alw26I GTCTC 2 cut(s) 248, 425
Alw44I GTGCAC 1 cut(s) 875
AlwI GGATC 6 cut(s) 64, 357, 370, 719, 732, 850
AoxI GGCC 2 cut(s) 562, 1148
ApaLI GTGCAC 1 cut(s) 875
ApeKI GCWGC 6 cut(s) 272, 903, 993, 1128, 1179, 1182
ApoI RAATTY 2 cut(s) 32, 587
AspLEI GCGC 1 cut(s) 975
AspS9I GGNCC 3 cut(s) 491, 1149, 1172
AsuHPI GGTGA 1 cut(s) 94
AsuII TTCGAA 1 cut(s) 226
AvaII GGWCC 2 cut(s) 491, 1172
BaeGI GKGCMC 3 cut(s) 647, 762, 879
BamHI GGATCC 2 cut(s) 362, 724
BanI GGYRCC 1 cut(s) 972
Bbv12I GWGCWC 2 cut(s) 17, 879
BbvI GCAGC 6 cut(s) 259, 890, 980, 1115, 1166, 1169
BccI CCATC 3 cut(s) 58, 804, 1244
BceAI ACGGC 1 cut(s) 109
BcgI CGANNNNNNTGC 2 cut(s) 912, 946
BciT130I CCWGG 3 cut(s) 158, 452, 970
BcoDI GTCTC 2 cut(s) 248, 425
BfaI CTAG 1 cut(s) 653
BfmI CTRYAG 1 cut(s) 904
BfoI RGCGCY 1 cut(s) 976
BisI GCNGC 7 cut(s) 273, 904, 994, 1129, 1148, 1180, 1183
BlsI GCNGC 7 cut(s) 274, 905, 995, 1130, 1149, 1181, 1184
Bme1390I CCNGG 3 cut(s) 158, 452, 970
Bme18I GGWCC 2 cut(s) 491, 1172
BmgT120I GGNCC 3 cut(s) 491, 1149, 1172
BmiI GGNNCC 4 cut(s) 202, 364, 726, 974
BmrFI CCNGG 3 cut(s) 158, 452, 970
BmsI GCATC 1 cut(s) 518
BoxI GACNNNNGTC 1 cut(s) 282
Bpu14I TTCGAA 1 cut(s) 226
BpuEI CTTGAG 2 cut(s) 296, 449
BsaHI GRCGYC 1 cut(s) 973
BsaJI CCNNGG 3 cut(s) 21, 156, 280
BsaXI ACNNNNNCTCC 2 cut(s) 483, 513
Bsc4I CCNNNNNNNGG 7 cut(s) 313, 820, 1020, 1044, 1068, 1092, 1116
Bse1I ACTGG 1 cut(s) 376
Bse3DI GCAATG 3 cut(s) 274, 706, 898
BseBI CCWGG 3 cut(s) 158, 452, 970
BseDI CCNNGG 3 cut(s) 21, 156, 280
BseGI GGATG 2 cut(s) 789, 1255
BseLI CCNNNNNNNGG 7 cut(s) 313, 820, 1020, 1044, 1068, 1092, 1116
BseMI GCAATG 3 cut(s) 274, 706, 898
BseMII CTCAG 1 cut(s) 337
BseNI ACTGG 1 cut(s) 376
BseRI GAGGAG 7 cut(s) 112, 976, 1003, 1027, 1051, 1075, 1099
BseSI GKGCMC 3 cut(s) 647, 762, 879
BseXI GCAGC 6 cut(s) 259, 890, 980, 1115, 1166, 1169
BsgI GTGCAG 2 cut(s) 527, 1188
BshFI GGCC 2 cut(s) 564, 1150
BshNI GGYRCC 1 cut(s) 972
BsiHKAI GWGCWC 2 cut(s) 17, 879
BsiSI CCGG 1 cut(s) 859
BslI CCNNNNNNNGG 7 cut(s) 313, 820, 1020, 1044, 1068, 1092, 1116
BsmAI GTCTC 2 cut(s) 248, 425
BsmBI CGTCTC 1 cut(s) 248
BsnI GGCC 2 cut(s) 564, 1150
Bsp119I TTCGAA 1 cut(s) 226
Bsp1286I GDGCHC 4 cut(s) 17, 647, 762, 879
Bsp143I GATC 7 cut(s) 69, 295, 362, 462, 724, 855, 884
BspACI CCGC 3 cut(s) 793, 1147, 1204
BspANI GGCC 2 cut(s) 564, 1150
BspCNI CTCAG 1 cut(s) 336
BspLI GGNNCC 4 cut(s) 202, 364, 726, 974
BspMAI CTGCAG 1 cut(s) 908
BspPI GGATC 6 cut(s) 64, 357, 370, 719, 732, 850
BspT104I TTCGAA 1 cut(s) 226
BspT107I GGYRCC 1 cut(s) 972
BsrDI GCAATG 3 cut(s) 274, 706, 898
BsrI ACTGG 1 cut(s) 376
BssECI CCNNGG 3 cut(s) 21, 156, 280
BssMI GATC 7 cut(s) 69, 295, 362, 462, 724, 855, 884
BssNI GRCGYC 1 cut(s) 973
BssT1I CCWWGG 2 cut(s) 21, 280
Bst2UI CCWGG 3 cut(s) 158, 452, 970
Bst4CI ACNGT 3 cut(s) 106, 548, 708
BstACI GRCGYC 1 cut(s) 973
BstAPI GCANNNNNTGC 1 cut(s) 868
BstBI TTCGAA 1 cut(s) 226
BstC8I GCNNGC 4 cut(s) 98, 377, 895, 1145
BstDEI CTNAG 1 cut(s) 323
BstF5I GGATG 2 cut(s) 789, 1255
BstH2I RGCGCY 1 cut(s) 976
BstHHI GCGC 1 cut(s) 975
BstKTI GATC 7 cut(s) 72, 298, 365, 465, 727, 858, 887
BstMAI GTCTC 2 cut(s) 248, 425
BstMBI GATC 7 cut(s) 69, 295, 362, 462, 724, 855, 884
BstNI CCWGG 3 cut(s) 158, 452, 970
BstNSI RCATGY 4 cut(s) 13, 871, 875, 1194
BstPAI GACNNNNGTC 1 cut(s) 282
BstSCI CCNGG 3 cut(s) 156, 450, 968
BstSFI CTRYAG 1 cut(s) 904
BstSLI GKGCMC 3 cut(s) 647, 762, 879
BstV1I GCAGC 6 cut(s) 259, 890, 980, 1115, 1166, 1169
BstX2I RGATCY 4 cut(s) 69, 362, 724, 855
BstYI RGATCY 4 cut(s) 69, 362, 724, 855
BsuRI GGCC 2 cut(s) 564, 1150
BtsCI GGATG 2 cut(s) 789, 1255
BtsI GCAGTG 1 cut(s) 760
BtsIMutI CAGTG 4 cut(s) 553, 760, 834, 1205
Cac8I GCNNGC 4 cut(s) 98, 377, 895, 1145
CfoI GCGC 1 cut(s) 975
Cfr13I GGNCC 3 cut(s) 491, 1149, 1172
Csp6I GTAC 1 cut(s) 666
CspCI CAANNNNNGTGG 2 cut(s) 178, 213
CviQI GTAC 1 cut(s) 666
DdeI CTNAG 1 cut(s) 323
DinI GGCGCC 1 cut(s) 974
DpnI GATC 7 cut(s) 71, 297, 364, 464, 726, 857, 886
DpnII GATC 7 cut(s) 69, 295, 362, 462, 724, 855, 884
DraIII CACNNNGTG 1 cut(s) 1229
EciI GGCGGA 1 cut(s) 782
Eco130I CCWWGG 2 cut(s) 21, 280
Eco47I GGWCC 2 cut(s) 491, 1172
EcoRII CCWGG 3 cut(s) 156, 450, 968
EcoT14I CCWWGG 2 cut(s) 21, 280
EcoT22I ATGCAT 1 cut(s) 873
EgeI GGCGCC 1 cut(s) 974
EheI GGCGCC 1 cut(s) 974
ErhI CCWWGG 2 cut(s) 21, 280
Esp3I CGTCTC 1 cut(s) 248
FauNDI CATATG 2 cut(s) 235, 397
Fnu4HI GCNGC 7 cut(s) 273, 904, 994, 1129, 1148, 1180, 1183
FokI GGATG 1 cut(s) 776
Fsp4HI GCNGC 7 cut(s) 273, 904, 994, 1129, 1148, 1180, 1183
FspBI CTAG 1 cut(s) 653
GlaI GCGC 1 cut(s) 974
GluI GCNGC 7 cut(s) 273, 904, 994, 1129, 1148, 1180, 1183
HaeII RGCGCY 1 cut(s) 976
HaeIII GGCC 2 cut(s) 564, 1150
HapII CCGG 1 cut(s) 859
HhaI GCGC 1 cut(s) 975
Hin1I GRCGYC 1 cut(s) 973
Hin6I GCGC 1 cut(s) 973
HinP1I GCGC 1 cut(s) 973
HincII GTYRAC 1 cut(s) 287
HindII GTYRAC 1 cut(s) 287
HpaII CCGG 1 cut(s) 859
HphI GGTGA 1 cut(s) 94
Hpy166II GTNNAC 3 cut(s) 287, 491, 877
Hpy188III TCNNGA 1 cut(s) 631
Hpy8I GTNNAC 3 cut(s) 287, 491, 877
Hpy99I CGWCG 3 cut(s) 95, 110, 176
HpyAV CCTTC 3 cut(s) 49, 226, 575
HpyCH4III ACNGT 3 cut(s) 106, 548, 708
HpyCH4IV ACGT 3 cut(s) 174, 196, 636
HpyF3I CTNAG 1 cut(s) 323
HpySE526I ACGT 3 cut(s) 174, 196, 636
Hsp92I GRCGYC 1 cut(s) 973
HspAI GCGC 1 cut(s) 973
KasI GGCGCC 1 cut(s) 972
Kzo9I GATC 7 cut(s) 69, 295, 362, 462, 724, 855, 884
LmnI GCTCC 9 cut(s) 206, 772, 971, 989, 1016, 1040, 1064, 1088, 1112
Lsp1109I GCAGC 6 cut(s) 259, 890, 980, 1115, 1166, 1169
LweI GCATC 1 cut(s) 518
MaeI CTAG 1 cut(s) 653
MaeII ACGT 3 cut(s) 174, 196, 636
MaeIII GTNAC 1 cut(s) 690
MalI GATC 7 cut(s) 71, 297, 364, 464, 726, 857, 886
MboI GATC 7 cut(s) 69, 295, 362, 462, 724, 855, 884
MboII GAAGA 4 cut(s) 34, 215, 467, 683
MflI RGATCY 4 cut(s) 69, 362, 724, 855
MhlI GDGCHC 4 cut(s) 17, 647, 762, 879
MluCI AATT 5 cut(s) 32, 552, 587, 685, 909
Mly113I GGCGCC 1 cut(s) 973
MmeI TCCRAC 1 cut(s) 463
Mph1103I ATGCAT 1 cut(s) 873
MseI TTAA 4 cut(s) 527, 585, 747, 912
MslI CAYNNNNRTG 3 cut(s) 267, 699, 872
MspI CCGG 1 cut(s) 859
MspR9I CCNGG 3 cut(s) 158, 452, 970
MvaI CCWGG 3 cut(s) 158, 452, 970
NarI GGCGCC 1 cut(s) 973
NdeI CATATG 2 cut(s) 235, 397
NdeII GATC 7 cut(s) 69, 295, 362, 462, 724, 855, 884
NlaIV GGNNCC 4 cut(s) 202, 364, 726, 974
NsiI ATGCAT 1 cut(s) 873
NspI RCATGY 4 cut(s) 13, 871, 875, 1194
NspV TTCGAA 1 cut(s) 226
PciI ACATGT 1 cut(s) 1190
PflMI CCANNNNNTGG 1 cut(s) 820
PkrI GCNGC 7 cut(s) 274, 905, 995, 1130, 1149, 1181, 1184
PluTI GGCGCC 1 cut(s) 976
PscI ACATGT 1 cut(s) 1190
PshAI GACNNNNGTC 1 cut(s) 282
PsiI TTATAA 1 cut(s) 612
Psp6I CCWGG 3 cut(s) 156, 450, 968
PspGI CCWGG 3 cut(s) 156, 450, 968
PspN4I GGNNCC 4 cut(s) 202, 364, 726, 974
PspPI GGNCC 3 cut(s) 491, 1149, 1172
PstI CTGCAG 1 cut(s) 908
PsuI RGATCY 4 cut(s) 69, 362, 724, 855
RsaI GTAC 1 cut(s) 667
RsaNI GTAC 1 cut(s) 666
RseI CAYNNNNRTG 3 cut(s) 267, 699, 872
SaqAI TTAA 4 cut(s) 527, 585, 747, 912
SatI GCNGC 7 cut(s) 273, 904, 994, 1129, 1148, 1180, 1183
Sau3AI GATC 7 cut(s) 69, 295, 362, 462, 724, 855, 884
Sau96I GGNCC 3 cut(s) 491, 1149, 1172
ScrFI CCNGG 3 cut(s) 158, 452, 970
SduI GDGCHC 4 cut(s) 17, 647, 762, 879
SfaNI GCATC 1 cut(s) 518
SfcI CTRYAG 1 cut(s) 904
SfoI GGCGCC 1 cut(s) 974
SfuI TTCGAA 1 cut(s) 226
SinI GGWCC 2 cut(s) 491, 1172
SmiMI CAYNNNNRTG 3 cut(s) 267, 699, 872
SmlI CTYRAG 2 cut(s) 311, 428
SmoI CTYRAG 2 cut(s) 311, 428
Sse9I AATT 5 cut(s) 32, 552, 587, 685, 909
SsiI CCGC 3 cut(s) 793, 1147, 1204
SspDI GGCGCC 1 cut(s) 972
SspMI CTAG 1 cut(s) 653
StyD4I CCNGG 3 cut(s) 156, 450, 968
StyI CCWWGG 2 cut(s) 21, 280
TaaI ACNGT 3 cut(s) 106, 548, 708
TaiI ACGT 3 cut(s) 177, 199, 639
TaqI TCGA 2 cut(s) 171, 226
TasI AATT 5 cut(s) 32, 552, 587, 685, 909
TauI GCSGC 1 cut(s) 1150
Tru1I TTAA 4 cut(s) 527, 585, 747, 912
Tru9I TTAA 4 cut(s) 527, 585, 747, 912
TscAI CASTG 4 cut(s) 553, 767, 841, 1205
TseI GCWGC 6 cut(s) 272, 903, 993, 1128, 1179, 1182
TspGWI ACGGA 2 cut(s) 717, 833
TspRI CASTG 4 cut(s) 553, 767, 841, 1205
Van91I CCANNNNNTGG 1 cut(s) 820
VneI GTGCAC 1 cut(s) 875
VpaK11BI GGWCC 2 cut(s) 491, 1172
XapI RAATTY 2 cut(s) 32, 587
XceI RCATGY 4 cut(s) 13, 871, 875, 1194
XspI CTAG 1 cut(s) 653
Zsp2I ATGCAT 1 cut(s) 873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.