MD16G1270400.v1.1

domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0011765
Physical Location & Seq
Forward (+)
35076151 .. 35084414
8264 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1270400.v1.1.491

Sequence Viewer

Length: 1164 bp
ATGGCCGTTCTGCCTGTTAATAACTCCTTGGATGTGTTGTTGATTGACACAAAGCGGGGTGATGAGGAGATCGTGAGTGCGTGGAGGCGAAGGCATTGTGGGGTGCGGGAGCAACTGCGGCGGAGGAGGGACTGGATCTCTTCCATTTATCTGTGGGTGTATCATCAGTCTCGACGTTGCACTCCGACGAGAGTTGTTGAGTTCGAAGCCAGATCCGCCGGTAATACACCACGGTGGAACTGCTCTTACGCCCTCCTCACTCTGCTTTACTCGCATGACAATGCTGCTGACCTCGGCCAGCTCTATGACCTCTTTCTCCAGCTCAACCTCTCTCTGCGGGTCAATCTCATTGGGTATGACTATTCTGGCTATGGAGCCTCTACTGGCAAGCCTAGTGAAACAAATACATATGCTGACATAGAGGCAGTATATGAGTGCCTTGGGACCGAGTACGGAGTTAGCCAGGAAGATTTGATCTTGTATGGGCAGTCAGTTGGAAGTGGACCAACACCGCACTTGGCAGCTAAGTTGCCAAGGCTGCGGGGTGTAGTTCTGCATAGTGCAATTCTTTCCGGGCTAAGTTGCCATGTGAAATTCACATTCTGCCTTGACGTTTATAAGAGCAACTCACTGAAGCTCGTTCTGTTCCTATTCTATGCTATTCGCCATTCTGATGGTGTGCAGAACTTCAATAAAATTAAGAAAGTGAAGTGCCCGGTGCTAGTAATACATGGTACAGATGATGATATCGTGAATTGGTTACATGGTAATGCGCCGTGGAAAATGGCAAGGGATCCATATGAGCCTTTGTGGATTAAAGGAGGTGGGCACTGCAACTTGGAGCTTTACCCTGATTACATCCGCCATCTTTGCAGGTTTATCCTAGGAATAGAGAACATAACCACAGAAATTCGTCTAAAAAAGATTCGACAGAATATGCATTTGCAGAAGAAAAGATCATGTGCAATTTGTGCAAGCATGTGTTGTAGAATAAAACTCTACCAACCTAAATGCCCTGAATGTTCAAGACCGAAGTGTCTAAAATTTCCTACATGCTGGAAGCCTAAGTGCATAAAATGTAGTTGGCGACCCAAATGCCCGAAATGTGCAGGACCAAGCTGCTGCTTTAGAAGCGTCACTGGCGATGCTGTGGTTGTGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DLH Hydrolase_4

Protein Analysis

388

Amino Acids

44.07

Weight (kDa)

9.16

Isoelectric Point (pI)

59.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011765)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32190 AT1G32190
fragaria_vesca FvH4_5g23110 FvH4_5g23110
malus_domestica MD06G1038600.v1.1 MD16G1270400.v1.1
prunus_persica Prupe.5G027400_v2.0.a1 Prupe.5G027400_v2.0.a1
pyrus_communis pycom06g03240
rosa_chinensis RchiOBHm_Chr7g0211991
rosa_laevigata RLG00000002947
rosa_multiflora Rmu_sc0004869.1_g000006
rosa_roxburghii Rroxscaffold_3G00247220
rosa_rugosa Rorug07G0129900
rosa_samantha Rh7AG262700 Rh7BG256800 Rh7CG279300 Rh7DG269500
rosa_wichuraiana Rw7G022420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 618
AasI GACNNNNNNGTC 1 cut(s) 1035
Acc36I ACCTGC 1 cut(s) 864
AciI CCGC 9 cut(s) 55, 106, 118, 121, 216, 337, 512, 541, 862
AclWI GGATC 4 cut(s) 143, 207, 788, 801
AcoI YGGCCR 2 cut(s) 3, 295
AcsI RAATTY 3 cut(s) 593, 909, 1043
AcuI CTGAAG 1 cut(s) 653
AfaI GTAC 2 cut(s) 452, 736
AgsI TTSAA 2 cut(s) 691, 1026
AjnI CCWGG 1 cut(s) 462
AleI CACNNNNGTG 1 cut(s) 232
AluBI AGCT 6 cut(s) 301, 322, 524, 637, 844, 1119
AluI AGCT 6 cut(s) 301, 322, 524, 637, 844, 1119
Alw26I GTCTC 1 cut(s) 174
AlwI GGATC 4 cut(s) 143, 207, 788, 801
AoxI GGCC 2 cut(s) 3, 295
ApeKI GCWGC 5 cut(s) 284, 521, 538, 1119, 1122
ApoI RAATTY 3 cut(s) 593, 909, 1043
AspA2I CCTAGG 1 cut(s) 883
AspLEI GCGC 1 cut(s) 775
AspS9I GGNCC 3 cut(s) 444, 503, 1112
AsuC2I CCSGG 2 cut(s) 574, 716
AsuHPI GGTGA 1 cut(s) 71
AsuII TTCGAA 1 cut(s) 204
AvaII GGWCC 3 cut(s) 444, 503, 1112
AvrII CCTAGG 1 cut(s) 883
BaeGI GKGCMC 2 cut(s) 716, 831
BamHI GGATCC 1 cut(s) 793
BbvI GCAGC 5 cut(s) 271, 525, 533, 1106, 1109
BccI CCATC 2 cut(s) 668, 873
BceAI ACGGC 1 cut(s) 760
BcgI CGANNNNNNTGC 2 cut(s) 1077, 1111
BciT130I CCWGG 1 cut(s) 464
BcnI CCSGG 2 cut(s) 574, 716
BcoDI GTCTC 1 cut(s) 174
BfaI CTAG 3 cut(s) 393, 722, 884
BfuAI ACCTGC 1 cut(s) 864
BisI GCNGC 6 cut(s) 119, 285, 522, 539, 1120, 1123
BlnI CCTAGG 1 cut(s) 883
BlsI GCNGC 6 cut(s) 120, 286, 523, 540, 1121, 1124
Bme1390I CCNGG 3 cut(s) 464, 574, 716
Bme18I GGWCC 3 cut(s) 444, 503, 1112
BmgT120I GGNCC 3 cut(s) 444, 503, 1112
BmiI GGNNCC 3 cut(s) 376, 445, 795
BmrFI CCNGG 3 cut(s) 464, 574, 716
BmsI GCATC 1 cut(s) 1135
BpmI CTGGAG 1 cut(s) 302
Bpu14I TTCGAA 1 cut(s) 204
BpuMI CCSGG 2 cut(s) 574, 716
BsaJI CCNNGG 7 cut(s) 27, 230, 292, 439, 533, 776, 883
Bse118I RCCGGY 1 cut(s) 218
Bse1I ACTGG 3 cut(s) 137, 388, 1144
BseBI CCWGG 1 cut(s) 464
BseDI CCNNGG 7 cut(s) 27, 230, 292, 439, 533, 776, 883
BseGI GGATG 2 cut(s) 37, 858
BseNI ACTGG 3 cut(s) 137, 388, 1144
BseRI GAGGAG 3 cut(s) 80, 139, 245
BseSI GKGCMC 2 cut(s) 716, 831
BseXI GCAGC 5 cut(s) 271, 525, 533, 1106, 1109
BsgI GTGCAG 2 cut(s) 701, 1128
BshFI GGCC 2 cut(s) 5, 297
BsiSI CCGG 3 cut(s) 219, 573, 716
BslFI GGGAC 2 cut(s) 143, 457
BsmAI GTCTC 1 cut(s) 174
BsmFI GGGAC 2 cut(s) 143, 457
BsnI GGCC 2 cut(s) 5, 297
Bsp119I TTCGAA 1 cut(s) 204
Bsp1286I GDGCHC 2 cut(s) 716, 831
Bsp143I GATC 6 cut(s) 69, 135, 212, 474, 793, 956
BspACI CCGC 9 cut(s) 55, 106, 118, 121, 216, 337, 512, 541, 862
BspANI GGCC 2 cut(s) 5, 297
BspLI GGNNCC 3 cut(s) 376, 445, 795
BspMI ACCTGC 1 cut(s) 864
BspPI GGATC 4 cut(s) 143, 207, 788, 801
BspT104I TTCGAA 1 cut(s) 204
BsrFI RCCGGY 1 cut(s) 218
BsrI ACTGG 3 cut(s) 137, 388, 1144
BssAI RCCGGY 1 cut(s) 218
BssECI CCNNGG 7 cut(s) 27, 230, 292, 439, 533, 776, 883
BssMI GATC 6 cut(s) 69, 135, 212, 474, 793, 956
BssT1I CCWWGG 4 cut(s) 27, 439, 533, 883
Bst2UI CCWGG 1 cut(s) 464
Bst4CI ACNGT 1 cut(s) 234
Bst6I CTCTTC 1 cut(s) 145
BstAPI GCANNNNNTGC 1 cut(s) 971
BstBI TTCGAA 1 cut(s) 204
BstC8I GCNNGC 3 cut(s) 299, 389, 976
BstDEI CTNAG 3 cut(s) 525, 578, 1065
BstDSI CCRYGG 2 cut(s) 230, 776
BstF5I GGATG 2 cut(s) 37, 858
BstHHI GCGC 1 cut(s) 775
BstKTI GATC 6 cut(s) 72, 138, 215, 477, 796, 959
BstMAI GTCTC 1 cut(s) 174
BstMBI GATC 6 cut(s) 69, 135, 212, 474, 793, 956
BstMWI GCNNNNNNNGC 8 cut(s) 118, 215, 271, 538, 870, 971, 1131, 1140
BstNI CCWGG 1 cut(s) 464
BstNSI RCATGY 2 cut(s) 982, 1056
BstSCI CCNGG 3 cut(s) 462, 572, 714
BstSLI GKGCMC 2 cut(s) 716, 831
BstV1I GCAGC 5 cut(s) 271, 525, 533, 1106, 1109
BstX2I RGATCY 3 cut(s) 135, 212, 793
BstXI CCANNNNNNTGG 1 cut(s) 674
BstYI RGATCY 3 cut(s) 135, 212, 793
BsuRI GGCC 2 cut(s) 5, 297
BtgI CCRYGG 2 cut(s) 230, 776
BtgZI GCGATG 1 cut(s) 1158
BtsCI GGATG 2 cut(s) 37, 858
BtsI GCAGTG 1 cut(s) 829
BtsIMutI CAGTG 3 cut(s) 629, 829, 1137
BveI ACCTGC 1 cut(s) 864
Cac8I GCNNGC 3 cut(s) 299, 389, 976
CfoI GCGC 1 cut(s) 775
Cfr10I RCCGGY 1 cut(s) 218
Cfr13I GGNCC 3 cut(s) 444, 503, 1112
CseI GACGC 1 cut(s) 1123
Csp6I GTAC 2 cut(s) 451, 735
CviAII CATG 7 cut(s) 275, 587, 731, 764, 960, 979, 1053
CviQI GTAC 2 cut(s) 451, 735
DdeI CTNAG 3 cut(s) 525, 578, 1065
DpnI GATC 6 cut(s) 71, 137, 214, 476, 795, 958
DpnII GATC 6 cut(s) 69, 135, 212, 474, 793, 956
DrdI GACNNNNNNGTC 1 cut(s) 1035
DseDI GACNNNNNNGTC 1 cut(s) 1035
EaeI YGGCCR 2 cut(s) 3, 295
Eam1104I CTCTTC 1 cut(s) 145
EarI CTCTTC 1 cut(s) 145
EciI GGCGGA 3 cut(s) 136, 205, 851
Eco130I CCWWGG 4 cut(s) 27, 439, 533, 883
Eco32I GATATC 1 cut(s) 748
Eco47I GGWCC 3 cut(s) 444, 503, 1112
Eco57I CTGAAG 1 cut(s) 653
EcoRII CCWGG 1 cut(s) 462
EcoRV GATATC 1 cut(s) 748
EcoT14I CCWWGG 4 cut(s) 27, 439, 533, 883
EcoT22I ATGCAT 1 cut(s) 942
ErhI CCWWGG 4 cut(s) 27, 439, 533, 883
FaeI CATG 7 cut(s) 278, 590, 734, 767, 963, 982, 1056
FaqI GGGAC 2 cut(s) 143, 457
FatI CATG 7 cut(s) 274, 586, 730, 763, 959, 978, 1052
FauI CCCGC 4 cut(s) 48, 99, 330, 534
FauNDI CATATG 2 cut(s) 409, 799
Fnu4HI GCNGC 6 cut(s) 119, 285, 522, 539, 1120, 1123
FokI GGATG 2 cut(s) 44, 845
Fsp4HI GCNGC 6 cut(s) 119, 285, 522, 539, 1120, 1123
FspBI CTAG 3 cut(s) 393, 722, 884
GlaI GCGC 1 cut(s) 774
GluI GCNGC 6 cut(s) 119, 285, 522, 539, 1120, 1123
GsuI CTGGAG 1 cut(s) 302
HaeIII GGCC 2 cut(s) 5, 297
HapII CCGG 3 cut(s) 219, 573, 716
HgaI GACGC 1 cut(s) 1123
HhaI GCGC 1 cut(s) 775
Hin1II CATG 7 cut(s) 278, 590, 734, 767, 963, 982, 1056
Hin6I GCGC 1 cut(s) 773
HinP1I GCGC 1 cut(s) 773
HinfI GANTC 1 cut(s) 925
HpaII CCGG 3 cut(s) 219, 573, 716
HphI GGTGA 1 cut(s) 71
Hpy166II GTNNAC 1 cut(s) 503
Hpy188I TCNGA 2 cut(s) 186, 673
Hpy188III TCNNGA 4 cut(s) 73, 171, 751, 1026
Hpy8I GTNNAC 1 cut(s) 503
Hpy99I CGWCG 2 cut(s) 177, 190
HpyAV CCTTC 1 cut(s) 84
HpyCH4III ACNGT 1 cut(s) 234
HpyCH4IV ACGT 2 cut(s) 175, 612
HpyF10VI GCNNNNNNNGC 8 cut(s) 118, 215, 271, 538, 870, 971, 1131, 1140
HpyF3I CTNAG 3 cut(s) 525, 578, 1065
HpySE526I ACGT 2 cut(s) 175, 612
Hsp92II CATG 7 cut(s) 278, 590, 734, 767, 963, 982, 1056
HspAI GCGC 1 cut(s) 773
Kzo9I GATC 6 cut(s) 69, 135, 212, 474, 793, 956
LmnI GCTCC 3 cut(s) 109, 374, 841
Lsp1109I GCAGC 5 cut(s) 271, 525, 533, 1106, 1109
LweI GCATC 1 cut(s) 1135
MaeI CTAG 3 cut(s) 393, 722, 884
MaeII ACGT 2 cut(s) 175, 612
MaeIII GTNAC 2 cut(s) 759, 1135
MalI GATC 6 cut(s) 71, 137, 214, 476, 795, 958
MboI GATC 6 cut(s) 69, 135, 212, 474, 793, 956
MboII GAAGA 3 cut(s) 132, 479, 961
MflI RGATCY 3 cut(s) 135, 212, 793
MhlI GDGCHC 2 cut(s) 716, 831
MluCI AATT 7 cut(s) 564, 593, 696, 754, 909, 966, 1043
MmeI TCCRAC 2 cut(s) 209, 475
Mph1103I ATGCAT 1 cut(s) 942
MseI TTAA 4 cut(s) 18, 699, 816, 1162
MslI CAYNNNNRTG 4 cut(s) 232, 279, 672, 768
MspI CCGG 3 cut(s) 219, 573, 716
MspR9I CCNGG 3 cut(s) 464, 574, 716
MvaI CCWGG 1 cut(s) 464
MwoI GCNNNNNNNGC 8 cut(s) 118, 215, 271, 538, 870, 971, 1131, 1140
NciI CCSGG 2 cut(s) 574, 716
NdeI CATATG 2 cut(s) 409, 799
NdeII GATC 6 cut(s) 69, 135, 212, 474, 793, 956
NlaIII CATG 7 cut(s) 278, 590, 734, 767, 963, 982, 1056
NlaIV GGNNCC 3 cut(s) 376, 445, 795
NmeAIII GCCGAG 1 cut(s) 273
NmuCI GTSAC 1 cut(s) 1135
NsiI ATGCAT 1 cut(s) 942
NspI RCATGY 2 cut(s) 982, 1056
NspV TTCGAA 1 cut(s) 204
OliI CACNNNNGTG 1 cut(s) 232
PfeI GAWTC 1 cut(s) 925
PkrI GCNGC 6 cut(s) 120, 286, 523, 540, 1121, 1124
PsiI TTATAA 1 cut(s) 618
Psp6I CCWGG 1 cut(s) 462
PspGI CCWGG 1 cut(s) 462
PspN4I GGNNCC 3 cut(s) 376, 445, 795
PspPI GGNCC 3 cut(s) 444, 503, 1112
PsrI GAACNNNNNNTAC 2 cut(s) 230, 262
PsuI RGATCY 3 cut(s) 135, 212, 793
RsaI GTAC 2 cut(s) 452, 736
RsaNI GTAC 2 cut(s) 451, 735
RseI CAYNNNNRTG 4 cut(s) 232, 279, 672, 768
SaqAI TTAA 4 cut(s) 18, 699, 816, 1162
SatI GCNGC 6 cut(s) 119, 285, 522, 539, 1120, 1123
Sau3AI GATC 6 cut(s) 69, 135, 212, 474, 793, 956
Sau96I GGNCC 3 cut(s) 444, 503, 1112
ScrFI CCNGG 3 cut(s) 464, 574, 716
SduI GDGCHC 2 cut(s) 716, 831
SfaNI GCATC 1 cut(s) 1135
SfuI TTCGAA 1 cut(s) 204
SinI GGWCC 3 cut(s) 444, 503, 1112
SmiMI CAYNNNNRTG 4 cut(s) 232, 279, 672, 768
Sse9I AATT 7 cut(s) 564, 593, 696, 754, 909, 966, 1043
SsiI CCGC 9 cut(s) 55, 106, 118, 121, 216, 337, 512, 541, 862
SspMI CTAG 3 cut(s) 393, 722, 884
StyD4I CCNGG 3 cut(s) 462, 572, 714
StyI CCWWGG 4 cut(s) 27, 439, 533, 883
TaaI ACNGT 1 cut(s) 234
TaiI ACGT 2 cut(s) 178, 615
TaqI TCGA 3 cut(s) 172, 204, 928
TaqII GACCGA 2 cut(s) 461, 1045
TasI AATT 7 cut(s) 564, 593, 696, 754, 909, 966, 1043
TauI GCSGC 1 cut(s) 121
TfiI GAWTC 1 cut(s) 925
Tru1I TTAA 4 cut(s) 18, 699, 816, 1162
Tru9I TTAA 4 cut(s) 18, 699, 816, 1162
TscAI CASTG 3 cut(s) 636, 836, 1144
TseFI GTSAC 1 cut(s) 1135
TseI GCWGC 5 cut(s) 284, 521, 538, 1119, 1122
Tsp45I GTSAC 1 cut(s) 1135
TspGWI ACGGA 1 cut(s) 468
TspRI CASTG 3 cut(s) 636, 836, 1144
VpaK11BI GGWCC 3 cut(s) 444, 503, 1112
XapI RAATTY 3 cut(s) 593, 909, 1043
XceI RCATGY 2 cut(s) 982, 1056
XmaJI CCTAGG 1 cut(s) 883
XspI CTAG 3 cut(s) 393, 722, 884
Zsp2I ATGCAT 1 cut(s) 942
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.