Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0001776

Physical Location & Seq

Genomic Coordinates Forward (+)

14490653 .. 14496844

Gene Structure 6192 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g23160.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3345 bp

ATGGCCTCAGAATCTCCAATCAACACTTCAACCGCGTCCCCTAGGCTTCGCCGTGTTAGCCAAACAAGCCATGATCTCGACAGTGACATTGGAGGTGTCCAATTCGCAACCTACACGGTCCAGATACCCTCCACACCAGACAACAACCCCATGTCAGCTTCCATGGAGTACTCGACTTCTCAGAGAGTGGAGGACCAATTCGTCTCCAGCTCCCTCTTCACAGGCGGCTACAATTGCGTCACGCAAGCCCAATTGAAAGAGAAGGTCATTGAATCCCAGACCAGCCATCATCCCCAGATGACTGGGGCAAAAGGGTCTTTCTGCGCCGTGCCGGGTTGTGATGCCAAGGTGGTGACTGATGAGAGGGGACTGGATATAGTCCCGTGTGAATGTGACTACAAGATTTGTATGGATTGTTTTAGGGATGCTGTGAGAAATGGTGACCACATGTGTCCTGGCTGCAAGGAGCCTTATAGGGAGCTGGATGTGACAGAGTATGCTGTGCATAATCGAAAGCAGTATCAGCAGCAGATGTCGAAAACTGAGAGGAGAATGTCCTTGATGAAGTCGACAAAGGTGATGGGGCGCCAGAGCAGCGAGTTTGATCATAACAAGTGGTTGTTTGAGACAAAGGGGAGTTACGGATATGGGAATGCTATTTGGCCAAAGGTTGATGTGGAAGGGAGTCAAGATGGGATTGGTGGTGATCCCATGGTGTTTCATGATAAGCAATGGAGGCCTCTTACGCGCAAGCTGAATATCTCTGCTGCAATTCTCAGTCCATATAGGCTTTTAATTTTCATTCGAATGGTGGTCCTTGGATTATTCTTGCAATGGAGAATCAGAAATCCAAATGAAGATGCTGTCTGGTTGTGGGCTATGTCAGTGGTTTGTGAAGTCTGGTTTGCCATCTCATGGCTACTTGATCAGCTTCCAAAGCTCTGTCCCATCAATCGCATTACCAATCTTGATGTTCTAAAAGAAAAATTTGAAACACCAAGCCTCAGCAATCCTACTGGAAAATCTGATCTCCCAGGCATAGACATTTTTGTCTCTACAGCAGATCCAGAGAAGGAACCGCCTCTTGTCACCGCAAACACAATTCTTTCAATTCTTGCAGCTGACTACCCTGTTGAGAAGCTTGCATGTTATGTTTCTGATGATGGAGGTGCACTTTTAACATTTGAGGCCATGGCAGAAGCTGCTAGTTTTGCCAACTTGTGGGTACCCTTCTGTCGGAAGCACAACATTGAACCAAGAAACCCAGAATCTTACTTCAGTCTGAAGAGAGATCCTTATAAGAACAAAGTACGGCAAGATTTTGTTAGAGACCGAAGGAGAGTGAAGCGTGAGTATGATGAATATAAGGTTAGAATCAATGGCCTTCCTGATTCAATTCGGAGACGTTCAGATGCCTACAATGCTAGGGATGAGATGAAGGCCATGAAACTTGGGAGAGCGATTGGAAATGATGAAGCAGTTGATAAGATAAAGATCCCAAAAGCAACTTGGATGGCTGATGGAACACACTGGCCTGGAACCTGGGCTTTTCCTGCCCCCGAGCATTCTAGGGGTGATCATGCTAGTATTATGCAGGTTATGTTGAAACCTCCTAGTGATGAACCACTGAAAGGAACAACAGTGGATTCAAATTCTATGGATCTAAGTGAAGTTGACATTCGTCTTCCAATGTTGGTTTATGTATCTCGTGAGAAGCGACCTGGCTATGATCACAACAAGAAGGCTGGTGCTATGAATGCCTTGGTTCGTGCCTCAGCTGTCATGTCTAATGGGCCCTTCATTCTCAACCTTGACTGTGATCACTACATCTACAATTCCCAAGCATTGAGAGAAGGCATGTGCTTCATGATGGATCGTGGTGGTGACCGCATTAGTTATGTCCAGTTTCCTCAGAGGTTTGAAGGAATAGACCCTTCTGACCGTTATGCCAACCACAACACTGTTTTCTTTGATGTCAACATGCGTGCACTTGATGGCATTCAGGGTCCAGTTTATGTTGGAACAGGTTGCCTCTTTCGACGAACTGCCCTATATGGATTTGATCCACCTCGAGTGAAGGATCGTAATGGTTGTTGCTGTGGTGGCCGCAATAAGAAATCTAAATCTTCAGTTGTTTCTGACCCCGACTCTCAAACAATAGAACTGGGAGACTGTGATGATGATAACGAAATGAATGCCATTATTCCTAAAAGGTTCGGGAACTCAAGTTTCCTTGTAGAGTCTATCCAAGTGGCAGAATTCCAAGGCCGTCCTCTTGCTGATCATCCTTCATTGAAGAATGGACGTCCACCAGGTGCTCTCACATTTCCTCGTGAGCTTCTTGATGCATCAACAGTTGCAGAGGCAATCAGTGTCATCTCTTGCTGGTATGAGGATAAGACTGAATGGGGCCAACGAATTGGGTGGATTTATGGTTCAGTAACTGAAGATGTTGTCACAGGTTATAGGATGCACAACAGAGGATGGAGGTCTGTTTATTGTGTAACAAAAAGAGATGCATTTCGAGGTACTGCCCCAATCAATCTCACTGATCGCCTTCACCAGGTTCTCCGATGGGCAACTGGTTCTGTCGAGATTTTCTTTTCTCGAAACAATGCTCTTTTCGCTAGCTCCAAAATGAAGTTTCTACAAAGGATAGCGTACCTCAATGTTGGAATGTACCCTTTCACCTCCTTTTTCCTCATTGTCTACTGCTTCCTTCCAGCACTTTCACTCATTTCGGGCCAGTTCATAGTTCAGACCCTCAACATAACCTTCCTGGTATACCTTTTAGGCATCACAGTGACCCTCATTCTTCTTGCTGTGCTCGAGATCAAGTGGTCTGGAATTGAACTAGAAGAGTGGTGGAGGAATGAGCAGTTTTGGTTGATTGGAGGAACAAGCGCCCATCTTGCAGCTGTGCTTCAAGGTCTGCTAAAGGTCATAGCAGGTATTGAGATCTCATTCACACTGACATCAAAATCTGGAGGTGATGATATAGATGATGAGTTTGTGGATCTTTATATCTTCAAATGGACATCACTGATGATACCACCAATTACAATCATGATGACAAACTTGATTGGAATAGCTATTGCTGTTTGCCGAACAGTATATAGTGTCATACCACAGTGGAGCAAATTGCTTGGAGGAGTGTTCTTCAGTTTTTGGGTGCTGGCACATCTCTATCCCTTTGCAAAAGGGCTTATGGGAAGACGAGGGAAGACACCAACCATTGTGTTTGTGTGGTCTGGTCTTACAGCAATCACCATATCTCTCCTTTGGGTGGCTATTAGTCCCCCATCAGGTACTAATCAAATTGGCGGTTCATTCCAGTTCCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cellulose_synt zf-RING_4

Protein Analysis

1115

Amino Acids

125.35

Weight (kDa)

7.22

Isoelectric Point (pI)

39.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_4	PF14570	110 - 159	6.4e-17	RING/Ubox like zinc-binding domain
Cellulose_synt	PF03552	347 - 1105	0.0	Cellulose synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001776)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G02730 AT1G32180 AT1G32180
fragaria_vesca	FvH4_5g23130 FvH4_5g23130 FvH4_5g23130 FvH4_5g23130 FvH4_5g23130 FvH4_5g23130 FvH4_5g23130 FvH4_5g23160 FvH4_5g23160
prunus_persica	Prupe.5G027500_v2.0.a1 Prupe.5G027500_v2.0.a1 Prupe.5G027500_v2.0.a1 Prupe.5G027500_v2.0.a1 Prupe.5G027700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr7g0212001 RchiOBHm_Chr7g0212061
rosa_laevigata	RLG00000002942 RLG00000002946
rosa_multiflora	Rmu_co8225350.1_g000001 Rmu_co8305165.1_g000001 Rmu_co8314167.1_g000001 Rmu_co8341615.1_g000001 Rmu_co8366393.1_g000001 Rmu_co8499369.1_g000001 Rmu_sc0002283.1_g000005 Rmu_sc0002283.1_g000014 Rmu_sc0014812.1_g000004 Rmu_sc0014812.1_g000013
rosa_roxburghii	Rroxscaffold_3G00247170 Rroxscaffold_3G00247200
rosa_rugosa	Rorug07G0130000.1 Rorug07G0130100 Rorug07G0130200.1 Rorug07G0130300
rosa_samantha	Rh7AG262900 Rh7AG263400 Rh7BG257000 Rh7BG257200 Rh7CG279500 Rh7CG279900 Rh7DG269700 Rh7DG270200
rosa_wichuraiana	Rw7G022430 Rw7G022460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1299
AasI	GACNNNNNNGTC	2 cut(s)	200, 1049
AatII	GACGTC	1 cut(s)	2308
Acc36I	ACCTGC	2 cut(s)	1585, 2939
Acc65I	GGTACC	1 cut(s)	1225
AccB1I	GGYRCC	2 cut(s)	585, 1225
AccB7I	CCANNNNNTGG	2 cut(s)	915, 1221
AccI	GTMKAC	3 cut(s)	569, 2709, 2784
AccII	CGCG	2 cut(s)	35, 748
AciI	CCGC	7 cut(s)	33, 225, 1079, 1092, 1888, 2107, 3324
AclWI	GGATC	9 cut(s)	701, 1058, 1286, 1489, 1668, 1881, 2057, 2088, 3024
AcoI	YGGCCR	2 cut(s)	662, 2104
AcsI	RAATTY	3 cut(s)	986, 1651, 2258
AcuI	CTGAAG	5 cut(s)	1261, 1304, 2112, 2466, 3145
AcyI	GRCGYC	2 cut(s)	586, 2305
AdeI	CACNNNGTG	1 cut(s)	2315
AfaI	GTAC	7 cut(s)	170, 1227, 1311, 2530, 2663, 2681, 3310
AfiI	CCNNNNNNNGG	7 cut(s)	915, 1221, 1236, 1631, 2563, 3286, 3305
AflIII	ACRYGT	1 cut(s)	447
AjnI	CCWGG	8 cut(s)	454, 1033, 1534, 1541, 1720, 2311, 2562, 2778
AjuI	GAANNNNNNNTTGG	2 cut(s)	10, 42
AloI	GAACNNNNNNTCC	2 cut(s)	2419, 2451
Alw21I	GWGCWC	4 cut(s)	1174, 1990, 2320, 2829
Alw26I	GTCTC	6 cut(s)	208, 620, 1057, 1323, 1396, 2163
Alw44I	GTGCAC	2 cut(s)	1170, 1986
AlwI	GGATC	9 cut(s)	701, 1058, 1286, 1489, 1668, 1881, 2057, 2088, 3024
AlwNI	CAGNNNCTG	2 cut(s)	1202, 2444
Ama87I	CYCGRG	3 cut(s)	1559, 2070, 2828
ApaI	GGGCCC	1 cut(s)	1797
ApaLI	GTGCAC	2 cut(s)	1170, 1986
ApeKI	GCWGC	7 cut(s)	459, 526, 594, 767, 1118, 1202, 2915
ApoI	RAATTY	3 cut(s)	986, 1651, 2258
Asp718I	GGTACC	1 cut(s)	1225
AspA2I	CCTAGG	1 cut(s)	41
AspLEI	GCGC	4 cut(s)	326, 588, 750, 2906
AspS9I	GGNCC	8 cut(s)	118, 193, 814, 1793, 1794, 2006, 2410, 2743
AsuC2I	CCSGG	1 cut(s)	333
AsuII	TTCGAA	1 cut(s)	805
AsuNHI	GCTAGC	1 cut(s)	2627
AvaI	CYCGRG	3 cut(s)	1559, 2070, 2828
AvaII	GGWCC	4 cut(s)	118, 193, 814, 2006
AvrII	CCTAGG	1 cut(s)	41
BaeGI	GKGCMC	3 cut(s)	1174, 1797, 1990
BalI	TGGCCA	1 cut(s)	664
BanI	GGYRCC	2 cut(s)	585, 1225
BanII	GRGCYC	1 cut(s)	1797
BauI	CACGAG	2 cut(s)	1707, 2331
BbsI	GAAGAC	3 cut(s)	1676, 3220, 3230
Bbv12I	GWGCWC	4 cut(s)	1174, 1990, 2320, 2829
BbvCI	CCTCAGC	2 cut(s)	1004, 1774
BbvI	GCAGC	7 cut(s)	446, 538, 606, 754, 1130, 1189, 2927
BceAI	ACGGC	4 cut(s)	36, 311, 1328, 2253
BcgI	CGANNNNNNTGC	2 cut(s)	2177, 2211
BciT130I	CCWGG	8 cut(s)	456, 1035, 1536, 1543, 1722, 2313, 2564, 2780
BclI	TGATCA	6 cut(s)	604, 925, 1576, 1729, 1819, 2281
BcnI	CCSGG	1 cut(s)	333
BcoDI	GTCTC	6 cut(s)	208, 620, 1057, 1323, 1396, 2163
BfaI	CTAG	8 cut(s)	42, 1206, 1425, 1569, 1584, 1614, 2628, 2855
BfmI	CTRYAG	1 cut(s)	1056
BfoI	RGCGCY	2 cut(s)	589, 2907
BfuAI	ACCTGC	2 cut(s)	1585, 2939
BglII	AGATCT	1 cut(s)	2958
BisI	GCNGC	9 cut(s)	226, 460, 527, 595, 768, 1119, 1203, 2107, 2916
BlnI	CCTAGG	1 cut(s)	41
BlsI	GCNGC	9 cut(s)	227, 461, 528, 596, 769, 1120, 1204, 2108, 2917
BmcAI	AGTACT	1 cut(s)	170
Bme1390I	CCNGG	9 cut(s)	333, 456, 1035, 1536, 1543, 1722, 2313, 2564, 2780
Bme18I	GGWCC	4 cut(s)	118, 193, 814, 2006
BmeT110I	CYCGRG	3 cut(s)	1559, 2070, 2828
BmgT120I	GGNCC	8 cut(s)	118, 193, 814, 1793, 1794, 2006, 2410, 2743
BmiI	GGNNCC	8 cut(s)	468, 587, 1077, 1227, 1540, 1795, 2007, 2411
BmrFI	CCNGG	9 cut(s)	333, 456, 1035, 1536, 1543, 1722, 2313, 2564, 2780
BmrI	ACTGGG	2 cut(s)	312, 2174
BmsI	GCATC	9 cut(s)	331, 415, 850, 1402, 2335, 2357, 2460, 2506, 2805
BmtI	GCTAGC	1 cut(s)	2631
BmuI	ACTGGG	2 cut(s)	312, 2174
BoxI	GACNNNNGTC	1 cut(s)	1680
BpiI	GAAGAC	3 cut(s)	1676, 3220, 3230
BpmI	CTGGAG	2 cut(s)	190, 3006
Bpu10I	CCTNAGC	2 cut(s)	1004, 1774
Bpu14I	TTCGAA	1 cut(s)	805
BpuEI	CTTGAG	1 cut(s)	2209
BpuMI	CCSGG	1 cut(s)	333
BsaBI	GATNNNNATC	1 cut(s)	1493
BsaHI	GRCGYC	2 cut(s)	586, 2305
BsaI	GGTCTC	1 cut(s)	1323
Bsc4I	CCNNNNNNNGG	7 cut(s)	915, 1221, 1236, 1631, 2563, 3286, 3305
Bse3DI	GCAATG	2 cut(s)	737, 839
Bse8I	GATNNNNATC	1 cut(s)	1493
BseBI	CCWGG	8 cut(s)	456, 1035, 1536, 1543, 1722, 2313, 2564, 2780
BseGI	GGATG	8 cut(s)	289, 430, 490, 1435, 1518, 2284, 2475, 2489
BseJI	GATNNNNATC	1 cut(s)	1493
BseLI	CCNNNNNNNGG	7 cut(s)	915, 1221, 1236, 1631, 2563, 3286, 3305
BseMI	GCAATG	2 cut(s)	737, 839
BseMII	CTCAG	7 cut(s)	21, 194, 534, 790, 1018, 1788, 1925
BseRI	GAGGAG	2 cut(s)	562, 3165
BseSI	GKGCMC	3 cut(s)	1174, 1797, 1990
BseXI	GCAGC	7 cut(s)	446, 538, 606, 754, 1130, 1189, 2927
Bsh1236I	CGCG	2 cut(s)	35, 748
BshNI	GGYRCC	2 cut(s)	585, 1225
BsiHKAI	GWGCWC	4 cut(s)	1174, 1990, 2320, 2829
BsiHKCI	CYCGRG	3 cut(s)	1559, 2070, 2828
BsiSI	CCGG	1 cut(s)	332
BslFI	GGGAC	5 cut(s)	22, 365, 381, 930, 3282
BslI	CCNNNNNNNGG	7 cut(s)	915, 1221, 1236, 1631, 2563, 3286, 3305
BsmAI	GTCTC	6 cut(s)	208, 620, 1057, 1323, 1396, 2163
BsmBI	CGTCTC	2 cut(s)	208, 1396
BsmFI	GGGAC	5 cut(s)	22, 365, 381, 930, 3282
BsmI	GAATGC	5 cut(s)	658, 1564, 1762, 1998, 2200
Bso31I	GGTCTC	1 cut(s)	1323
BsoBI	CYCGRG	3 cut(s)	1559, 2070, 2828
Bsp119I	TTCGAA	1 cut(s)	805
Bsp120I	GGGCCC	1 cut(s)	1793
Bsp1286I	GDGCHC	5 cut(s)	1174, 1797, 1990, 2320, 2829
Bsp19I	CCATGG	3 cut(s)	162, 711, 1191
BspACI	CCGC	7 cut(s)	33, 225, 1079, 1092, 1888, 2107, 3324
BspCNI	CTCAG	7 cut(s)	20, 193, 535, 789, 1017, 1787, 1924
BspFNI	CGCG	2 cut(s)	35, 748
BspHI	TCATGA	3 cut(s)	721, 1866, 3066
BspLI	GGNNCC	8 cut(s)	468, 587, 1077, 1227, 1540, 1795, 2007, 2411
BspMI	ACCTGC	2 cut(s)	1585, 2939
BspOI	GCTAGC	1 cut(s)	2631
BspPI	GGATC	9 cut(s)	701, 1058, 1286, 1489, 1668, 1881, 2057, 2088, 3024
BspT104I	TTCGAA	1 cut(s)	805
BspT107I	GGYRCC	2 cut(s)	585, 1225
BspTNI	GGTCTC	1 cut(s)	1323
BsrDI	GCAATG	2 cut(s)	737, 839
BssNAI	GTATAC	1 cut(s)	2785
BssNI	GRCGYC	2 cut(s)	586, 2305
BssSI	CACGAG	2 cut(s)	1707, 2331
BssT1I	CCWWGG	8 cut(s)	41, 162, 345, 711, 817, 1191, 1761, 2263
Bst1107I	GTATAC	1 cut(s)	2785
Bst2BI	CACGAG	2 cut(s)	1707, 2331
Bst2UI	CCWGG	8 cut(s)	456, 1035, 1536, 1543, 1722, 2313, 2564, 2780
Bst6I	CTCTTC	3 cut(s)	221, 1280, 2853
BstACI	GRCGYC	2 cut(s)	586, 2305
BstAPI	GCANNNNNTGC	1 cut(s)	1202
BstBI	TTCGAA	1 cut(s)	805
BstC8I	GCNNGC	6 cut(s)	246, 752, 1143, 1986, 2629, 3177
BstDEI	CTNAG	8 cut(s)	7, 180, 543, 776, 1004, 1664, 1774, 1911
BstDSI	CCRYGG	3 cut(s)	162, 711, 1191
BstEII	GGTNACC	2 cut(s)	440, 1883
BstENI	CCTNNNNNAGG	1 cut(s)	2561
BstF5I	GGATG	8 cut(s)	289, 430, 490, 1435, 1518, 2284, 2475, 2489
BstFNI	CGCG	2 cut(s)	35, 748
BstH2I	RGCGCY	2 cut(s)	589, 2907
BstHHI	GCGC	4 cut(s)	326, 588, 750, 2906
BstMAI	GTCTC	6 cut(s)	208, 620, 1057, 1323, 1396, 2163
BstNI	CCWGG	8 cut(s)	456, 1035, 1536, 1543, 1722, 2313, 2564, 2780
BstNSI	RCATGY	4 cut(s)	451, 1149, 1861, 1984
BstPAI	GACNNNNGTC	1 cut(s)	1680
BstPI	GGTNACC	2 cut(s)	440, 1883
BstSCI	CCNGG	9 cut(s)	331, 454, 1033, 1534, 1541, 1720, 2311, 2562, 2778
BstSFI	CTRYAG	1 cut(s)	1056
BstSLI	GKGCMC	3 cut(s)	1174, 1797, 1990
BstUI	CGCG	2 cut(s)	35, 748
BstV1I	GCAGC	7 cut(s)	446, 538, 606, 754, 1130, 1189, 2927
BstV2I	GAAGAC	3 cut(s)	1676, 3220, 3230
BstX2I	RGATCY	6 cut(s)	1063, 1291, 1494, 1660, 2958, 3016
BstXI	CCANNNNNNTGG	2 cut(s)	302, 2420
BstYI	RGATCY	6 cut(s)	1063, 1291, 1494, 1660, 2958, 3016
BstZ17I	GTATAC	1 cut(s)	2785
BtgI	CCRYGG	3 cut(s)	162, 711, 1191
BtsCI	GGATG	8 cut(s)	289, 430, 490, 1435, 1518, 2284, 2475, 2489
BveI	ACCTGC	2 cut(s)	1585, 2939
Cac8I	GCNNGC	6 cut(s)	246, 752, 1143, 1986, 2629, 3177
CaiI	CAGNNNCTG	2 cut(s)	1202, 2444
CciI	TCATGA	3 cut(s)	721, 1866, 3066
CfoI	GCGC	4 cut(s)	326, 588, 750, 2906
Cfr13I	GGNCC	8 cut(s)	118, 193, 814, 1793, 1794, 2006, 2410, 2743
CseI	GACGC	2 cut(s)	24, 226
CsiI	ACCWGGT	2 cut(s)	2311, 2562
Csp6I	GTAC	7 cut(s)	169, 1226, 1310, 2529, 2662, 2680, 3309
CviQI	GTAC	7 cut(s)	169, 1226, 1310, 2529, 2662, 2680, 3309
DdeI	CTNAG	8 cut(s)	7, 180, 543, 776, 1004, 1664, 1774, 1911
DinI	GGCGCC	1 cut(s)	587
DraIII	CACNNNGTG	1 cut(s)	2315
DrdI	GACNNNNNNGTC	2 cut(s)	200, 1049
DseDI	GACNNNNNNGTC	2 cut(s)	200, 1049
EaeI	YGGCCR	2 cut(s)	662, 2104
Eam1104I	CTCTTC	3 cut(s)	221, 1280, 2853
EarI	CTCTTC	3 cut(s)	221, 1280, 2853
Eco130I	CCWWGG	8 cut(s)	41, 162, 345, 711, 817, 1191, 1761, 2263
Eco147I	AGGCCT	1 cut(s)	739
Eco24I	GRGCYC	1 cut(s)	1797
Eco31I	GGTCTC	1 cut(s)	1323
Eco47I	GGWCC	4 cut(s)	118, 193, 814, 2006
Eco57I	CTGAAG	5 cut(s)	1261, 1304, 2112, 2466, 3145
Eco88I	CYCGRG	3 cut(s)	1559, 2070, 2828
Eco91I	GGTNACC	2 cut(s)	440, 1883
EcoNI	CCTNNNNNAGG	1 cut(s)	2561
EcoO109I	RGGNCCY	1 cut(s)	1794
EcoO65I	GGTNACC	2 cut(s)	440, 1883
EcoRI	GAATTC	1 cut(s)	2258
EcoRII	CCWGG	8 cut(s)	454, 1033, 1534, 1541, 1720, 2311, 2562, 2778
EcoT14I	CCWWGG	8 cut(s)	41, 162, 345, 711, 817, 1191, 1761, 2263
EcoT22I	ATGCAT	2 cut(s)	2350, 2521
EcoT38I	GRGCYC	1 cut(s)	1797
EgeI	GGCGCC	1 cut(s)	587
EheI	GGCGCC	1 cut(s)	587
ErhI	CCWWGG	8 cut(s)	41, 162, 345, 711, 817, 1191, 1761, 2263
Esp3I	CGTCTC	2 cut(s)	208, 1396
FaqI	GGGAC	5 cut(s)	22, 365, 381, 930, 3282
FbaI	TGATCA	6 cut(s)	604, 925, 1576, 1729, 1819, 2281
FblI	GTMKAC	3 cut(s)	569, 2709, 2784
Fnu4HI	GCNGC	9 cut(s)	226, 460, 527, 595, 768, 1119, 1203, 2107, 2916
FokI	GGATG	8 cut(s)	276, 437, 497, 1442, 1525, 2271, 2482, 2496
FriOI	GRGCYC	1 cut(s)	1797
Fsp4HI	GCNGC	9 cut(s)	226, 460, 527, 595, 768, 1119, 1203, 2107, 2916
FspBI	CTAG	8 cut(s)	42, 1206, 1425, 1569, 1584, 1614, 2628, 2855
GlaI	GCGC	4 cut(s)	325, 587, 749, 2905
GluI	GCNGC	9 cut(s)	226, 460, 527, 595, 768, 1119, 1203, 2107, 2916
GsuI	CTGGAG	2 cut(s)	190, 3006
HaeII	RGCGCY	2 cut(s)	589, 2907
HapII	CCGG	1 cut(s)	332
HgaI	GACGC	2 cut(s)	24, 226
HhaI	GCGC	4 cut(s)	326, 588, 750, 2906
Hin1I	GRCGYC	2 cut(s)	586, 2305
Hin6I	GCGC	4 cut(s)	324, 586, 748, 2904
HinP1I	GCGC	4 cut(s)	324, 586, 748, 2904
HincII	GTYRAC	3 cut(s)	570, 1675, 1978
HindII	GTYRAC	3 cut(s)	570, 1675, 1978
HindIII	AAGCTT	1 cut(s)	1139
HpaII	CCGG	1 cut(s)	332
Hpy166II	GTNNAC	8 cut(s)	570, 1172, 1675, 1978, 1988, 2309, 2710, 2785
Hpy8I	GTNNAC	8 cut(s)	570, 1172, 1675, 1978, 1988, 2309, 2710, 2785
Hpy99I	CGWCG	1 cut(s)	2043
HpyCH4IV	ACGT	2 cut(s)	1405, 2305
HpyF3I	CTNAG	8 cut(s)	7, 180, 543, 776, 1004, 1664, 1774, 1911
HpySE526I	ACGT	2 cut(s)	1405, 2305
Hsp92I	GRCGYC	2 cut(s)	586, 2305
HspAI	GCGC	4 cut(s)	324, 586, 748, 2904
KasI	GGCGCC	1 cut(s)	585
KpnI	GGTACC	1 cut(s)	1229
Ksp22I	TGATCA	6 cut(s)	604, 925, 1576, 1729, 1819, 2281
LmnI	GCTCC	5 cut(s)	215, 466, 478, 2636, 3135
Lsp1109I	GCAGC	7 cut(s)	446, 538, 606, 754, 1130, 1189, 2927
LweI	GCATC	9 cut(s)	331, 415, 850, 1402, 2335, 2357, 2460, 2506, 2805
MabI	ACCWGGT	2 cut(s)	2311, 2562
MaeI	CTAG	8 cut(s)	42, 1206, 1425, 1569, 1584, 1614, 2628, 2855
MaeII	ACGT	2 cut(s)	1405, 2305
MfeI	CAATTG	2 cut(s)	232, 251
MflI	RGATCY	6 cut(s)	1063, 1291, 1494, 1660, 2958, 3016
MhlI	GDGCHC	5 cut(s)	1174, 1797, 1990, 2320, 2829
MlsI	TGGCCA	1 cut(s)	664
MluNI	TGGCCA	1 cut(s)	664
Mly113I	GGCGCC	1 cut(s)	586
MlyI	GAGTC	3 cut(s)	694, 2141, 2249
MmeI	TCCRAC	3 cut(s)	1217, 1999, 2653
Mox20I	TGGCCA	1 cut(s)	664
Mph1103I	ATGCAT	2 cut(s)	2350, 2521
MscI	TGGCCA	1 cut(s)	664
MseI	TTAA	2 cut(s)	794, 1178
MslI	CAYNNNNRTG	2 cut(s)	806, 2801
Msp20I	TGGCCA	1 cut(s)	664
MspA1I	CMGCKG	3 cut(s)	1121, 1778, 2918
MspI	CCGG	1 cut(s)	332
MspR9I	CCNGG	9 cut(s)	333, 456, 1035, 1536, 1543, 1722, 2313, 2564, 2780
MunI	CAATTG	2 cut(s)	232, 251
Mva1269I	GAATGC	5 cut(s)	658, 1564, 1762, 1998, 2200
MvaI	CCWGG	8 cut(s)	456, 1035, 1536, 1543, 1722, 2313, 2564, 2780
MvnI	CGCG	2 cut(s)	35, 748
NarI	GGCGCC	1 cut(s)	586
NciI	CCSGG	1 cut(s)	333
NcoI	CCATGG	3 cut(s)	162, 711, 1191
NheI	GCTAGC	1 cut(s)	2627
NlaIV	GGNNCC	8 cut(s)	468, 587, 1077, 1227, 1540, 1795, 2007, 2411
NsiI	ATGCAT	2 cut(s)	2350, 2521
NspI	RCATGY	4 cut(s)	451, 1149, 1861, 1984
NspV	TTCGAA	1 cut(s)	805
PaeR7I	CTCGAG	2 cut(s)	2070, 2828
PagI	TCATGA	3 cut(s)	721, 1866, 3066
PceI	AGGCCT	1 cut(s)	739
PciI	ACATGT	1 cut(s)	447
PcsI	WCGNNNNNNNCGW	1 cut(s)	1714
PctI	GAATGC	5 cut(s)	658, 1564, 1762, 1998, 2200
PfeI	GAWTC	7 cut(s)	11, 272, 840, 1268, 1374, 1391, 1646
PflMI	CCANNNNNTGG	2 cut(s)	915, 1221
PkrI	GCNGC	9 cut(s)	227, 461, 528, 596, 769, 1120, 1204, 2108, 2917
PleI	GAGTC	3 cut(s)	693, 2141, 2248
PluTI	GGCGCC	1 cut(s)	589
PpsI	GAGTC	3 cut(s)	693, 2141, 2248
PscI	ACATGT	1 cut(s)	447
PshAI	GACNNNNGTC	1 cut(s)	1680
PsiI	TTATAA	1 cut(s)	1299
Psp6I	CCWGG	8 cut(s)	454, 1033, 1534, 1541, 1720, 2311, 2562, 2778
PspEI	GGTNACC	2 cut(s)	440, 1883
PspGI	CCWGG	8 cut(s)	454, 1033, 1534, 1541, 1720, 2311, 2562, 2778
PspN4I	GGNNCC	8 cut(s)	468, 587, 1077, 1227, 1540, 1795, 2007, 2411
PspOMI	GGGCCC	1 cut(s)	1793
PspPI	GGNCC	8 cut(s)	118, 193, 814, 1793, 1794, 2006, 2410, 2743
PspXI	VCTCGAGB	1 cut(s)	2070
PstNI	CAGNNNCTG	2 cut(s)	1202, 2444
PsuI	RGATCY	6 cut(s)	1063, 1291, 1494, 1660, 2958, 3016
PvuII	CAGCTG	3 cut(s)	1121, 1778, 2918
RsaI	GTAC	7 cut(s)	170, 1227, 1311, 2530, 2663, 2681, 3310
RsaNI	GTAC	7 cut(s)	169, 1226, 1310, 2529, 2662, 2680, 3309
RseI	CAYNNNNRTG	2 cut(s)	806, 2801
SalI	GTCGAC	1 cut(s)	568
SaqAI	TTAA	2 cut(s)	794, 1178
SatI	GCNGC	9 cut(s)	226, 460, 527, 595, 768, 1119, 1203, 2107, 2916
Sau96I	GGNCC	8 cut(s)	118, 193, 814, 1793, 1794, 2006, 2410, 2743
ScaI	AGTACT	1 cut(s)	170
SchI	GAGTC	3 cut(s)	694, 2141, 2249
ScrFI	CCNGG	9 cut(s)	333, 456, 1035, 1536, 1543, 1722, 2313, 2564, 2780
SduI	GDGCHC	5 cut(s)	1174, 1797, 1990, 2320, 2829
SexAI	ACCWGGT	2 cut(s)	2311, 2562
SfaNI	GCATC	9 cut(s)	331, 415, 850, 1402, 2335, 2357, 2460, 2506, 2805
SfcI	CTRYAG	1 cut(s)	1056
SfoI	GGCGCC	1 cut(s)	587
Sfr274I	CTCGAG	2 cut(s)	2070, 2828
SfuI	TTCGAA	1 cut(s)	805
SinI	GGWCC	4 cut(s)	118, 193, 814, 2006
SlaI	CTCGAG	2 cut(s)	2070, 2828
SmiMI	CAYNNNNRTG	2 cut(s)	806, 2801
SmlI	CTYRAG	3 cut(s)	2070, 2224, 2828
SmoI	CTYRAG	3 cut(s)	2070, 2224, 2828
SseBI	AGGCCT	1 cut(s)	739
SsiI	CCGC	7 cut(s)	33, 225, 1079, 1092, 1888, 2107, 3324
SspDI	GGCGCC	1 cut(s)	585
SspMI	CTAG	8 cut(s)	42, 1206, 1425, 1569, 1584, 1614, 2628, 2855
StuI	AGGCCT	1 cut(s)	739
StyD4I	CCNGG	9 cut(s)	331, 454, 1033, 1534, 1541, 1720, 2311, 2562, 2778
StyI	CCWWGG	8 cut(s)	41, 162, 345, 711, 817, 1191, 1761, 2263
TaiI	ACGT	2 cut(s)	1408, 2308
TaqII	GACCGA	1 cut(s)	1347
TatI	WGTACW	1 cut(s)	168
TauI	GCSGC	2 cut(s)	228, 2109
TfiI	GAWTC	7 cut(s)	11, 272, 840, 1268, 1374, 1391, 1646
Tru1I	TTAA	2 cut(s)	794, 1178
Tru9I	TTAA	2 cut(s)	794, 1178
TseI	GCWGC	7 cut(s)	459, 526, 594, 767, 1118, 1202, 2915
TspGWI	ACGGA	1 cut(s)	657
Van91I	CCANNNNNTGG	2 cut(s)	915, 1221
VneI	GTGCAC	2 cut(s)	1170, 1986
VpaK11BI	GGWCC	4 cut(s)	118, 193, 814, 2006
XagI	CCTNNNNNAGG	1 cut(s)	2561
XapI	RAATTY	3 cut(s)	986, 1651, 2258
XceI	RCATGY	4 cut(s)	451, 1149, 1861, 1984
XcmI	CCANNNNNNNNNTGG	2 cut(s)	452, 1506
XhoI	CTCGAG	2 cut(s)	2070, 2828
XmaJI	CCTAGG	1 cut(s)	41
XmiI	GTMKAC	3 cut(s)	569, 2709, 2784
XspI	CTAG	8 cut(s)	42, 1206, 1425, 1569, 1584, 1614, 2628, 2855
ZraI	GACGTC	1 cut(s)	2306
ZrmI	AGTACT	1 cut(s)	170
Zsp2I	ATGCAT	2 cut(s)	2350, 2521

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.