Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0001776

Physical Location & Seq

Genomic Coordinates Reverse (-)

42162266 .. 42167044

Gene Structure 4779 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000002946

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3444 bp

ATGAGAAGAAGTGGGGTTTGCTTTGGAAGTGTTGTAGAGTTTAGAAAAATTGGTGGATTGGATACTCTTGTGAACATGATGGCCTCTAATTACCCAAATGGATCCTCCAGGTTTAACCATCTTAAGCAAACTAGCAATGACCTTGACAGTGATGATATTGGGAGTGTTGAGTTTGCAACCTACACTGTCCAGATACCCGTCACGCCGGACAACCAGCCCACCGGAATTTCCTTGCAGCGGTCAACTTCTCAGAGAGTTGAGGACCAATACGCGTCCAGTTCTTTGTTCACAGGTGGCTACAACTGCGTTACACGAGCTCAGTTGAAGGACAAAGTGATTGAATCCGAAACAAGCCATCCCCAGATGACATGGGCTAAGGGGTCTTATTGTACCGTGCCTGGATGTGATGCTCAGGTGGTGACGGATGAGAGGGGATTGGATATAGTCCCTTGTGAATGCGGTTTCAAGATTTGTATGGATTGTTATAGGGATGCTATGAGAAATGGTGAGAGTGTTTGTCCGGGCTGCAGAGAACCTTATAAGGAGATGGACATGAATGAGTACGGTGTTACTCGTAGGCAGCCGTTGCTGCCAACGGATGCGATGTCAAAGACGGACAGGAGGATGTCGCTTATGAAATCAAAATCGATGAAGTCAAAAGCGTTGGTGGAGAGTCAGGCTATTGAAATGGATCAGAATCAGTGGTTGTTTGAGACGAAGGGGAGTTATGGATATGGGAATGCTATGTGGCCAAAGGAAGATGTGAGTGGGAGTCTTGATGGGATGAGTGGTGATCTTAAAATGTTCAAAGATAAGCAATGGAAGCCACTAACGCGTGAAGTGAAAATCTCGGCTGCAATTCTCAGTCCATATCGGCTTCTAGTACTAGCTCGACTGGCGGTCCTTGGATTATTTTTGTATTGGAGAGTCACAAACCCTAATGATGATGCAGTGTGGTTGTGGCTTATGTCTGTGATTTGTGAATTCTGGTTTGCCTTCTCTTGGATACTTGATCAGCTTCCAAAGCTGTGCCCCATAAATCGCATTGCTTATGTTGATGTTTTAAAGGAGAAGTTTGAAACTCCAAATCCAAGCAATCCTACTGGAAAATCTGATCTTCCAGGAATAGACATTTTTGTATCTACAGCAGATCCAGAGAAAGAACCTCCTCTTGTAACTGCAAACACGATTCTCTCGATACTTGCAACTGACTACCCTGTTGAGAAGCTTGCCTGTTATGTTTCTGATGATGGAGGTGCACTTTTGACCTTTGAGGCCATGGCAGAAGCTGCTAGTTTTGCCAACTTGTGGGTACCATTTTGTCGGAAGCATGATATTGAGCCAAGGAACCCAGAATCTTACTTCAGCATGAAGAGAGATCCTTACAAGAATAAAGTAAAGTCCGATTTTGTAAGGGATCGCAGGCAGGTAAAGCGAGAGTATGATGAATTTAAGGTCAGGATTAATGGCCTTCCTGATTCAATCCGGAGACGTTCTGATGCCTTTAATGCTAGAGAGGAGATCAAAGCTATGAAACTTCGGAGAGAGACTAGAGACGATGAACCGCTGGAAAATTTAAAGTTCCCAAAAGCAACTTGGATGACTGATGGAACACAATGGCCTGGTACTTGGGTAGTTTCTGCACCTGAGCATTCCAAGGGTGATCATGCCAGTATCATACAGGTGATGTTAAAACCCCCTAGCGATGAGCCACTTAAAGGAACAGAAGTAGATTCAAATTCCATGAATCTAAGTGAGGTTGATATCCGTCTTCCCATGCTTGTTTATGTTTCTCGTGAAAAGAGGCCTGGTTATGATCATAACAAGAAAGCCGGAGCTATGAATGCCTTGGTTCGAGCCTCAGCCGTCATGTCCAATGGCGCCTTCATTCTCAATCTTGATTGTGACCACTACATCTACTACTCCCAAGCAATGAGAGAAGGCATGTGCTTCATGATGGACCGCGGTGGTGAACGCCTTTGTTATGTCCAATTTCCTCAGAGGTTTGAAGGAATAGACCCTTCTGATCGCTATGCCAACCACAACACTGTTTTCTTTGACGTCAACATGCGTGCTCTTGATGGAATTCAGGGTCCAGTGTATGTGGGAACAGGATGTCTCTTTCGTCGGACTGCCTTATATGGCTTTGATCCACCTCCAATGAAAGAACGTGATGGTTGCTGTAGTGGTTTCTTTGCCAAGCGCAAGAAAACTTCTTCAGTTGCTTCTGCTCCTGAAGTTGACTCCCAAGACGGATCAGTTGAACTAGACAATGACAATGAAGAAATGGGTCTTGCTCTTATTCCTAAAACATTTGGGAACTCAAGCCTCTTTGTTGACTCCATCCGAGTAGCAGAGTTCCAAGGTCGGCCCCTTGCTGATCATCCATCCATGAAAAATGGACGCCCCATTGGTGCCCTTACTCTTCCCCGGGAACTTCTTGATGCATCAACAGTTGCAGAGGCAATTAGTTGCATCTCATGCTGGTATGAGGACAAGACTGAATGGGGCCAACGAGTTGGGTGGATTTATGGTTCAGTGACTGAAGATGTGGTCACAGGTTACAGGATGCACAACCGTGGATGGAAATCTATCTACTGTGTCACTAAGAAGGATGCCTTTCGAGGAAGTGCCCCAATCAATCTCACTGACCGGCTTCACCAGGTTTTGCGGTGGGCTACTGGCTCTGTTGAAATATTCTTTTCTCGTAACAATGCTCTTCTAGCCAGCTCCAGGATGAAATTTCTCCAAAGGATTGCCTACTTGAATGTCGGAATGTACCCTTTCACATCCATTTTCCTCATTGTTTACTGCTTCCTTCCTGCACTCTCACTCTTTACTGATCAGTTCATTGTTCAGCAACTAAATGTAACCTTCCTGGTATATCTCTTGGGCATTACAATTACCCTCATTCTTCTTGCCGTGCTTGAGATCAAGTGGTCCGGAATTGAACTTGAGGAGTGGTGGAGGAATGAGCAGTTTTGGTTGATTGGAGGCACAAGTGCCCACCTTGCTGCTGTGCTCCAAGGTCTTCTAAAGGTTATAGCAGGAATTGAGATCTCATTCACTTTGACATCAAAATCGGGAGGCGATGATGCAGATGATGACTTTGTTGATCTTTATATCTTCAAGTGGACATCTCTGATGGTACCACCTATTACTATCATAATGGTTAACTTGATTGGAATAGGTGTTGCTGTTTGTCGAACAATATACAGTGATAACCCAAAGTGGAGCAGCTTGCTTGGAGGTTCTTTTTTCAGCTTCTGGGTGTTGGCTCATCTATACCCCTTCGCCAAAGGACTTATGGGAAGACGAGGGAGGACCCCCACGATTGTATTTGTCTGGTCTGGGCTTCTAGCAATCACCATATCTCTCCTTTGGGTGGCTATTGATCCCCCATCAGGCAGTAGTGGAATTGGAGGTTCATTCCAGTTTCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cellulose_synt zf-RING_4

Protein Analysis

1148

Amino Acids

128.98

Weight (kDa)

6.05

Isoelectric Point (pI)

36.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_4	PF14570	132 - 181	3.7e-18	RING/Ubox like zinc-binding domain
Cellulose_synt	PF03552	376 - 1135	0.0	Cellulose synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001776)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G02730 AT1G32180 AT1G32180
fragaria_vesca	FvH4_5g23130 FvH4_5g23130 FvH4_5g23130 FvH4_5g23130 FvH4_5g23130 FvH4_5g23130 FvH4_5g23130 FvH4_5g23160 FvH4_5g23160
prunus_persica	Prupe.5G027500_v2.0.a1 Prupe.5G027500_v2.0.a1 Prupe.5G027500_v2.0.a1 Prupe.5G027500_v2.0.a1 Prupe.5G027700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr7g0212001 RchiOBHm_Chr7g0212061
rosa_laevigata	RLG00000002942 RLG00000002946
rosa_multiflora	Rmu_co8225350.1_g000001 Rmu_co8305165.1_g000001 Rmu_co8314167.1_g000001 Rmu_co8341615.1_g000001 Rmu_co8366393.1_g000001 Rmu_co8499369.1_g000001 Rmu_sc0002283.1_g000005 Rmu_sc0002283.1_g000014 Rmu_sc0014812.1_g000004 Rmu_sc0014812.1_g000013
rosa_roxburghii	Rroxscaffold_3G00247170 Rroxscaffold_3G00247200
rosa_rugosa	Rorug07G0130000.1 Rorug07G0130100 Rorug07G0130200.1 Rorug07G0130300
rosa_samantha	Rh7AG262900 Rh7AG263400 Rh7BG257000 Rh7BG257200 Rh7CG279500 Rh7CG279900 Rh7DG269700 Rh7DG270200
rosa_wichuraiana	Rw7G022430 Rw7G022460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	540
AatII	GACGTC	1 cut(s)	2064
Acc36I	ACCTGC	1 cut(s)	1417
Acc65I	GGTACC	2 cut(s)	1312, 3148
AccB1I	GGYRCC	4 cut(s)	1312, 1880, 2414, 3148
AccB7I	CCANNNNNTGG	1 cut(s)	1308
AccII	CGCG	3 cut(s)	272, 835, 1965
AccIII	TCCGGA	2 cut(s)	1485, 2942
AciI	CCGC	7 cut(s)	238, 459, 899, 1565, 1963, 1965, 2671
AclWI	GGATC	9 cut(s)	96, 109, 699, 1145, 1373, 1425, 2144, 2263, 3389
AcoI	YGGCCR	1 cut(s)	749
AcsI	RAATTY	7 cut(s)	225, 983, 1448, 1573, 1738, 2085, 2741
AcuI	CTGAAG	4 cut(s)	1348, 2202, 2256, 2565
AcyI	GRCGYC	3 cut(s)	1881, 2061, 2404
AfaI	GTAC	7 cut(s)	391, 563, 885, 1314, 1627, 2780, 3150
AfiI	CCNNNNNNNGG	7 cut(s)	237, 1002, 1308, 1718, 2733, 3385, 3404
AflII	CTTAAG	1 cut(s)	122
AflIII	ACRYGT	2 cut(s)	270, 833
AhdI	GACNNNNNGTC	1 cut(s)	899
AjnI	CCWGG	8 cut(s)	107, 397, 1120, 1621, 1807, 2661, 2732, 2877
AjuI	GAANNNNNNNTTGG	2 cut(s)	1868, 1900
AleI	CACNNNNGTG	1 cut(s)	2577
AloI	GAACNNNNNNTCC	2 cut(s)	2518, 2550
Alw21I	GWGCWC	4 cut(s)	319, 1261, 2077, 3024
Alw26I	GTCTC	5 cut(s)	707, 1483, 1541, 1548, 2123
Alw44I	GTGCAC	1 cut(s)	1257
AlwI	GGATC	9 cut(s)	96, 109, 699, 1145, 1373, 1425, 2144, 2263, 3389
AlwNI	CAGNNNCTG	3 cut(s)	1289, 2543, 3267
Ama87I	CYCGRG	1 cut(s)	2430
Aor13HI	TCCGGA	2 cut(s)	1485, 2942
AoxI	GGCC	8 cut(s)	81, 749, 1275, 1468, 1619, 1805, 2369, 2509
ApaLI	GTGCAC	1 cut(s)	1257
ApeKI	GCWGC	8 cut(s)	235, 525, 580, 589, 854, 1289, 3014, 3237
ApoI	RAATTY	7 cut(s)	225, 983, 1448, 1573, 1738, 2085, 2741
AseI	ATTAAT	1 cut(s)	1464
Asp718I	GGTACC	2 cut(s)	1312, 3148
AspLEI	GCGC	2 cut(s)	1883, 2205
AspS9I	GGNCC	8 cut(s)	262, 901, 1960, 2093, 2370, 2509, 2940, 3324
AsuC2I	CCSGG	3 cut(s)	522, 2431, 2432
AsuHPI	GGTGA	8 cut(s)	430, 518, 803, 1673, 1696, 1982, 2651, 3358
AvaI	CYCGRG	1 cut(s)	2430
AvaII	GGWCC	6 cut(s)	262, 901, 1960, 2093, 2940, 3324
BaeGI	GKGCMC	5 cut(s)	1034, 1261, 2419, 2635, 3007
BaeI	ACNNNNGTAYC	4 cut(s)	54, 87, 1617, 1650
BalI	TGGCCA	1 cut(s)	751
BamHI	GGATCC	1 cut(s)	101
BanI	GGYRCC	4 cut(s)	1312, 1880, 2414, 3148
BanII	GRGCYC	1 cut(s)	319
BauI	CACGAG	2 cut(s)	312, 1794
BbsI	GAAGAC	3 cut(s)	1763, 3023, 3319
Bbv12I	GWGCWC	4 cut(s)	319, 1261, 2077, 3024
BbvCI	CCTCAGC	1 cut(s)	1861
BbvI	GCAGC	8 cut(s)	247, 512, 576, 592, 841, 1276, 3001, 3249
BceAI	ACGGC	3 cut(s)	568, 1850, 2906
BciT130I	CCWGG	8 cut(s)	109, 399, 1122, 1623, 1809, 2663, 2734, 2879
BciVI	GTATCC	2 cut(s)	55, 999
BclI	TGATCA	5 cut(s)	1012, 1663, 1816, 2380, 2842
BcnI	CCSGG	3 cut(s)	522, 2431, 2432
BcoDI	GTCTC	5 cut(s)	707, 1483, 1541, 1548, 2123
BfmI	CTRYAG	3 cut(s)	526, 1143, 2182
BfoI	RGCGCY	1 cut(s)	1884
BfrI	CTTAAG	1 cut(s)	122
BfuAI	ACCTGC	1 cut(s)	1417
BfuI	GTATCC	2 cut(s)	55, 999
BglII	AGATCT	1 cut(s)	3057
BisI	GCNGC	8 cut(s)	236, 526, 581, 590, 855, 1290, 3015, 3238
BlsI	GCNGC	8 cut(s)	237, 527, 582, 591, 856, 1291, 3016, 3239
BmcAI	AGTACT	1 cut(s)	885
Bme18I	GGWCC	6 cut(s)	262, 901, 1960, 2093, 2940, 3324
BmeRI	GACNNNNNGTC	1 cut(s)	899
BmeT110I	CYCGRG	1 cut(s)	2430
BmgT120I	GGNCC	8 cut(s)	262, 901, 1960, 2093, 2370, 2509, 2940, 3324
BpiI	GAAGAC	3 cut(s)	1763, 3023, 3319
BpmI	CTGGAG	2 cut(s)	91, 2716
Bpu10I	CCTNAGC	4 cut(s)	375, 411, 1647, 1861
BpuEI	CTTGAG	3 cut(s)	2308, 2948, 2975
BpuMI	CCSGG	3 cut(s)	522, 2431, 2432
Bsa29I	ATCGAT	1 cut(s)	647
BsaBI	GATNNNNATC	2 cut(s)	696, 2587
BsaHI	GRCGYC	3 cut(s)	1881, 2061, 2404
BsaWI	WCCGGW	3 cut(s)	221, 1485, 2942
Bsc4I	CCNNNNNNNGG	7 cut(s)	237, 1002, 1308, 1718, 2733, 3385, 3404
Bse118I	RCCGGY	1 cut(s)	2652
Bse1I	ACTGG	7 cut(s)	276, 900, 1108, 1671, 2096, 2686, 3433
Bse3DI	GCAATG	4 cut(s)	142, 824, 1044, 1938
Bse8I	GATNNNNATC	2 cut(s)	696, 2587
BseAI	TCCGGA	2 cut(s)	1485, 2942
BseBI	CCWGG	8 cut(s)	109, 399, 1122, 1623, 1809, 2663, 2734, 2879
BseCI	ATCGAT	1 cut(s)	647
BseJI	GATNNNNATC	2 cut(s)	696, 2587
BseLI	CCNNNNNNNGG	7 cut(s)	237, 1002, 1308, 1718, 2733, 3385, 3404
BseMI	GCAATG	4 cut(s)	142, 824, 1044, 1938
BseMII	CTCAG	7 cut(s)	263, 332, 425, 877, 1638, 1875, 2012
BseNI	ACTGG	7 cut(s)	276, 900, 1108, 1671, 2096, 2686, 3433
BseRI	GAGGAG	3 cut(s)	1158, 1532, 2972
BseSI	GKGCMC	5 cut(s)	1034, 1261, 2419, 2635, 3007
BseXI	GCAGC	8 cut(s)	247, 512, 576, 592, 841, 1276, 3001, 3249
BsgI	GTGCAG	2 cut(s)	1626, 2808
Bsh1236I	CGCG	3 cut(s)	272, 835, 1965
BshFI	GGCC	8 cut(s)	83, 751, 1277, 1470, 1621, 1807, 2371, 2511
BshNI	GGYRCC	4 cut(s)	1312, 1880, 2414, 3148
BshVI	ATCGAT	1 cut(s)	647
BsiHKAI	GWGCWC	4 cut(s)	319, 1261, 2077, 3024
BsiHKCI	CYCGRG	1 cut(s)	2430
BsiSI	CCGG	8 cut(s)	206, 222, 521, 1486, 1833, 2431, 2653, 2943
BslFI	GGGAC	1 cut(s)	431
BslI	CCNNNNNNNGG	7 cut(s)	237, 1002, 1308, 1718, 2733, 3385, 3404
BsmAI	GTCTC	5 cut(s)	707, 1483, 1541, 1548, 2123
BsmBI	CGTCTC	3 cut(s)	707, 1483, 1548
BsmFI	GGGAC	1 cut(s)	431
BsmI	GAATGC	4 cut(s)	461, 745, 1651, 1849
BsnI	GGCC	8 cut(s)	83, 751, 1277, 1470, 1621, 1807, 2371, 2511
BsoBI	CYCGRG	1 cut(s)	2430
Bsp1286I	GDGCHC	8 cut(s)	319, 1034, 1261, 2077, 2419, 2635, 3007, 3024
Bsp13I	TCCGGA	2 cut(s)	1485, 2942
Bsp19I	CCATGG	1 cut(s)	1278
BspACI	CCGC	7 cut(s)	238, 459, 899, 1565, 1963, 1965, 2671
BspANI	GGCC	8 cut(s)	83, 751, 1277, 1470, 1621, 1807, 2371, 2511
BspCNI	CTCAG	7 cut(s)	262, 331, 424, 876, 1639, 1874, 2011
BspDI	ATCGAT	1 cut(s)	647
BspEI	TCCGGA	2 cut(s)	1485, 2942
BspFNI	CGCG	3 cut(s)	272, 835, 1965
BspHI	TCATGA	1 cut(s)	1953
BspMAI	CTGCAG	1 cut(s)	530
BspMI	ACCTGC	1 cut(s)	1417
BspPI	GGATC	9 cut(s)	96, 109, 699, 1145, 1373, 1425, 2144, 2263, 3389
BspQI	GCTCTTC	1 cut(s)	2724
BspT107I	GGYRCC	4 cut(s)	1312, 1880, 2414, 3148
BspTI	CTTAAG	1 cut(s)	122
BsrDI	GCAATG	4 cut(s)	142, 824, 1044, 1938
BsrFI	RCCGGY	1 cut(s)	2652
BsrI	ACTGG	7 cut(s)	276, 900, 1108, 1671, 2096, 2686, 3433
BssAI	RCCGGY	1 cut(s)	2652
BssNI	GRCGYC	3 cut(s)	1881, 2061, 2404
BssSI	CACGAG	2 cut(s)	312, 1794
BssT1I	CCWWGG	7 cut(s)	904, 1278, 1343, 1656, 1848, 2362, 3025
Bst2BI	CACGAG	2 cut(s)	312, 1794
Bst2UI	CCWGG	8 cut(s)	109, 399, 1122, 1623, 1809, 2663, 2734, 2879
Bst4CI	ACNGT	9 cut(s)	149, 187, 394, 566, 2050, 2455, 2579, 2600, 3218
Bst6I	CTCTTC	3 cut(s)	1367, 2430, 2724
BstACI	GRCGYC	3 cut(s)	1881, 2061, 2404
BstAFI	CTTAAG	1 cut(s)	122
BstAPI	GCANNNNNTGC	3 cut(s)	586, 1289, 2481
BstC8I	GCNNGC	5 cut(s)	1230, 1424, 2073, 2728, 3242
BstDSI	CCRYGG	3 cut(s)	1278, 1963, 2578
BstFNI	CGCG	3 cut(s)	272, 835, 1965
BstH2I	RGCGCY	1 cut(s)	1884
BstHHI	GCGC	2 cut(s)	1883, 2205
BstMAI	GTCTC	5 cut(s)	707, 1483, 1541, 1548, 2123
BstNI	CCWGG	8 cut(s)	109, 399, 1122, 1623, 1809, 2663, 2734, 2879
BstNSI	RCATGY	2 cut(s)	1948, 2071
BstSFI	CTRYAG	3 cut(s)	526, 1143, 2182
BstSLI	GKGCMC	5 cut(s)	1034, 1261, 2419, 2635, 3007
BstUI	CGCG	3 cut(s)	272, 835, 1965
BstV1I	GCAGC	8 cut(s)	247, 512, 576, 592, 841, 1276, 3001, 3249
BstV2I	GAAGAC	3 cut(s)	1763, 3023, 3319
BstX2I	RGATCY	4 cut(s)	101, 1150, 1378, 3057
BstXI	CCANNNNNNTGG	1 cut(s)	2519
BstYI	RGATCY	4 cut(s)	101, 1150, 1378, 3057
Bsu15I	ATCGAT	1 cut(s)	647
BsuI	GTATCC	2 cut(s)	55, 999
BsuRI	GGCC	8 cut(s)	83, 751, 1277, 1470, 1621, 1807, 2371, 2511
BsuTUI	ATCGAT	1 cut(s)	647
BtgI	CCRYGG	3 cut(s)	1278, 1963, 2578
BtgZI	GCGATG	3 cut(s)	617, 1719, 3105
BtsI	GCAGTG	1 cut(s)	957
BtsIMutI	CAGTG	9 cut(s)	154, 183, 707, 957, 2046, 2103, 2544, 2646, 3223
BveI	ACCTGC	1 cut(s)	1417
Cac8I	GCNNGC	5 cut(s)	1230, 1424, 2073, 2728, 3242
CaiI	CAGNNNCTG	3 cut(s)	1289, 2543, 3267
CciI	TCATGA	1 cut(s)	1953
CfoI	GCGC	2 cut(s)	1883, 2205
Cfr10I	RCCGGY	1 cut(s)	2652
Cfr13I	GGNCC	8 cut(s)	262, 901, 1960, 2093, 2370, 2509, 2940, 3324
Cfr42I	CCGCGG	1 cut(s)	1966
Cfr9I	CCCGGG	1 cut(s)	2430
ClaI	ATCGAT	1 cut(s)	647
CseI	GACGC	2 cut(s)	261, 2412
CsiI	ACCWGGT	1 cut(s)	2661
Csp6I	GTAC	7 cut(s)	390, 562, 884, 1313, 1626, 2779, 3149
CviQI	GTAC	7 cut(s)	390, 562, 884, 1313, 1626, 2779, 3149
DinI	GGCGCC	1 cut(s)	1882
DraI	TTTAAA	2 cut(s)	1065, 1578
DriI	GACNNNNNGTC	1 cut(s)	899
EaeI	YGGCCR	1 cut(s)	749
Eam1104I	CTCTTC	3 cut(s)	1367, 2430, 2724
Eam1105I	GACNNNNNGTC	1 cut(s)	899
EarI	CTCTTC	3 cut(s)	1367, 2430, 2724
Ecl136II	GAGCTC	1 cut(s)	317
Eco130I	CCWWGG	7 cut(s)	904, 1278, 1343, 1656, 1848, 2362, 3025
Eco147I	AGGCCT	1 cut(s)	1807
Eco24I	GRGCYC	1 cut(s)	319
Eco32I	GATATC	1 cut(s)	1765
Eco47I	GGWCC	6 cut(s)	262, 901, 1960, 2093, 2940, 3324
Eco53kI	GAGCTC	1 cut(s)	317
Eco57I	CTGAAG	4 cut(s)	1348, 2202, 2256, 2565
Eco88I	CYCGRG	1 cut(s)	2430
EcoICRI	GAGCTC	1 cut(s)	317
EcoO109I	RGGNCCY	1 cut(s)	3324
EcoRI	GAATTC	2 cut(s)	983, 2085
EcoRII	CCWGG	8 cut(s)	107, 397, 1120, 1621, 1807, 2661, 2732, 2877
EcoRV	GATATC	1 cut(s)	1765
EcoT14I	CCWWGG	7 cut(s)	904, 1278, 1343, 1656, 1848, 2362, 3025
EcoT22I	ATGCAT	1 cut(s)	2449
EcoT38I	GRGCYC	1 cut(s)	319
EgeI	GGCGCC	1 cut(s)	1882
EheI	GGCGCC	1 cut(s)	1882
ErhI	CCWWGG	7 cut(s)	904, 1278, 1343, 1656, 1848, 2362, 3025
Esp3I	CGTCTC	3 cut(s)	707, 1483, 1548
FalI	AAGNNNNNCTT	2 cut(s)	2603, 2635
FaqI	GGGAC	1 cut(s)	431
FbaI	TGATCA	5 cut(s)	1012, 1663, 1816, 2380, 2842
Fnu4HI	GCNGC	8 cut(s)	236, 526, 581, 590, 855, 1290, 3015, 3238
FriOI	GRGCYC	1 cut(s)	319
Fsp4HI	GCNGC	8 cut(s)	236, 526, 581, 590, 855, 1290, 3015, 3238
GlaI	GCGC	2 cut(s)	1882, 2204
GluI	GCNGC	8 cut(s)	236, 526, 581, 590, 855, 1290, 3015, 3238
GsuI	CTGGAG	2 cut(s)	91, 2716
HaeII	RGCGCY	1 cut(s)	1884
HaeIII	GGCC	8 cut(s)	83, 751, 1277, 1470, 1621, 1807, 2371, 2511
HapII	CCGG	8 cut(s)	206, 222, 521, 1486, 1833, 2431, 2653, 2943
HgaI	GACGC	2 cut(s)	261, 2412
HhaI	GCGC	2 cut(s)	1883, 2205
Hin1I	GRCGYC	3 cut(s)	1881, 2061, 2404
Hin6I	GCGC	2 cut(s)	1881, 2203
HinP1I	GCGC	2 cut(s)	1881, 2203
HincII	GTYRAC	5 cut(s)	243, 2065, 2242, 2338, 3175
HindII	GTYRAC	5 cut(s)	243, 2065, 2242, 2338, 3175
HindIII	AAGCTT	1 cut(s)	1226
HpaI	GTTAAC	1 cut(s)	3175
HpaII	CCGG	8 cut(s)	206, 222, 521, 1486, 1833, 2431, 2653, 2943
HphI	GGTGA	8 cut(s)	430, 518, 803, 1673, 1696, 1982, 2651, 3358
Hpy99I	CGWCG	1 cut(s)	2130
HpyCH4III	ACNGT	9 cut(s)	149, 187, 394, 566, 2050, 2455, 2579, 2600, 3218
HpyCH4IV	ACGT	3 cut(s)	1492, 2061, 2170
HpySE526I	ACGT	3 cut(s)	1492, 2061, 2170
Hsp92I	GRCGYC	3 cut(s)	1881, 2061, 2404
HspAI	GCGC	2 cut(s)	1881, 2203
KasI	GGCGCC	1 cut(s)	1880
Kpn2I	TCCGGA	2 cut(s)	1485, 2942
KpnI	GGTACC	2 cut(s)	1316, 3152
Ksp22I	TGATCA	5 cut(s)	1012, 1663, 1816, 2380, 2842
KspAI	GTTAAC	1 cut(s)	3175
KspI	CCGCGG	1 cut(s)	1966
LguI	GCTCTTC	1 cut(s)	2724
LmnI	GCTCC	5 cut(s)	1835, 2236, 2735, 3027, 3234
Lsp1109I	GCAGC	8 cut(s)	247, 512, 576, 592, 841, 1276, 3001, 3249
MabI	ACCWGGT	1 cut(s)	2661
MaeII	ACGT	3 cut(s)	1492, 2061, 2170
MflI	RGATCY	4 cut(s)	101, 1150, 1378, 3057
MhlI	GDGCHC	8 cut(s)	319, 1034, 1261, 2077, 2419, 2635, 3007, 3024
MlsI	TGGCCA	1 cut(s)	751
MluI	ACGCGT	2 cut(s)	270, 833
MluNI	TGGCCA	1 cut(s)	751
Mly113I	GGCGCC	1 cut(s)	1881
MlyI	GAGTC	5 cut(s)	682, 781, 936, 2237, 2333
MmeI	TCCRAC	3 cut(s)	1304, 2108, 2752
Mox20I	TGGCCA	1 cut(s)	751
Mph1103I	ATGCAT	1 cut(s)	2449
MroI	TCCGGA	2 cut(s)	1485, 2942
MscI	TGGCCA	1 cut(s)	751
MslI	CAYNNNNRTG	2 cut(s)	1682, 2577
Msp20I	TGGCCA	1 cut(s)	751
MspA1I	CMGCKG	3 cut(s)	238, 1567, 1965
MspCI	CTTAAG	1 cut(s)	122
MspI	CCGG	8 cut(s)	206, 222, 521, 1486, 1833, 2431, 2653, 2943
Mva1269I	GAATGC	4 cut(s)	461, 745, 1651, 1849
MvaI	CCWGG	8 cut(s)	109, 399, 1122, 1623, 1809, 2663, 2734, 2879
MvnI	CGCG	3 cut(s)	272, 835, 1965
NarI	GGCGCC	1 cut(s)	1881
NciI	CCSGG	3 cut(s)	522, 2431, 2432
NcoI	CCATGG	1 cut(s)	1278
NmeAIII	GCCGAG	1 cut(s)	830
NmuCI	GTSAC	7 cut(s)	199, 418, 928, 1904, 2539, 2554, 2602
NsiI	ATGCAT	1 cut(s)	2449
NspI	RCATGY	2 cut(s)	1948, 2071
OliI	CACNNNNGTG	1 cut(s)	2577
PagI	TCATGA	1 cut(s)	1953
PceI	AGGCCT	1 cut(s)	1807
PciSI	GCTCTTC	1 cut(s)	2724
PcsI	WCGNNNNNNNCGW	1 cut(s)	1193
PctI	GAATGC	4 cut(s)	461, 745, 1651, 1849
PfeI	GAWTC	7 cut(s)	341, 697, 1189, 1355, 1478, 1733, 1747
PflMI	CCANNNNNTGG	1 cut(s)	1308
PfoI	TCCNGGA	2 cut(s)	1120, 2732
PkrI	GCNGC	8 cut(s)	237, 527, 582, 591, 856, 1291, 3016, 3239
PleI	GAGTC	5 cut(s)	681, 780, 935, 2237, 2333
PluTI	GGCGCC	1 cut(s)	1884
PpsI	GAGTC	5 cut(s)	681, 780, 935, 2237, 2333
PpuMI	RGGWCCY	1 cut(s)	3324
PshBI	ATTAAT	1 cut(s)	1464
PsiI	TTATAA	1 cut(s)	540
Psp124BI	GAGCTC	1 cut(s)	319
Psp5II	RGGWCCY	1 cut(s)	3324
Psp6I	CCWGG	8 cut(s)	107, 397, 1120, 1621, 1807, 2661, 2732, 2877
PspGI	CCWGG	8 cut(s)	107, 397, 1120, 1621, 1807, 2661, 2732, 2877
PspPI	GGNCC	8 cut(s)	262, 901, 1960, 2093, 2370, 2509, 2940, 3324
PspPPI	RGGWCCY	1 cut(s)	3324
PstI	CTGCAG	1 cut(s)	530
PstNI	CAGNNNCTG	3 cut(s)	1289, 2543, 3267
PsuI	RGATCY	4 cut(s)	101, 1150, 1378, 3057
RsaI	GTAC	7 cut(s)	391, 563, 885, 1314, 1627, 2780, 3150
RsaNI	GTAC	7 cut(s)	390, 562, 884, 1313, 1626, 2779, 3149
RseI	CAYNNNNRTG	2 cut(s)	1682, 2577
SacI	GAGCTC	1 cut(s)	319
SacII	CCGCGG	1 cut(s)	1966
SapI	GCTCTTC	1 cut(s)	2724
SatI	GCNGC	8 cut(s)	236, 526, 581, 590, 855, 1290, 3015, 3238
Sau96I	GGNCC	8 cut(s)	262, 901, 1960, 2093, 2370, 2509, 2940, 3324
ScaI	AGTACT	1 cut(s)	885
SchI	GAGTC	5 cut(s)	682, 781, 936, 2237, 2333
SduI	GDGCHC	8 cut(s)	319, 1034, 1261, 2077, 2419, 2635, 3007, 3024
SexAI	ACCWGGT	1 cut(s)	2661
SfcI	CTRYAG	3 cut(s)	526, 1143, 2182
SfoI	GGCGCC	1 cut(s)	1882
Sfr303I	CCGCGG	1 cut(s)	1966
SgrBI	CCGCGG	1 cut(s)	1966
SinI	GGWCC	6 cut(s)	262, 901, 1960, 2093, 2940, 3324
SmaI	CCCGGG	1 cut(s)	2432
SmiMI	CAYNNNNRTG	2 cut(s)	1682, 2577
SmlI	CTYRAG	4 cut(s)	122, 2323, 2927, 2954
SmoI	CTYRAG	4 cut(s)	122, 2323, 2927, 2954
SseBI	AGGCCT	1 cut(s)	1807
SsiI	CCGC	7 cut(s)	238, 459, 899, 1565, 1963, 1965, 2671
SspDI	GGCGCC	1 cut(s)	1880
SspI	AATATT	1 cut(s)	2697
SstI	GAGCTC	1 cut(s)	319
StuI	AGGCCT	1 cut(s)	1807
StyI	CCWWGG	7 cut(s)	904, 1278, 1343, 1656, 1848, 2362, 3025
TaaI	ACNGT	9 cut(s)	149, 187, 394, 566, 2050, 2455, 2579, 2600, 3218
TaiI	ACGT	3 cut(s)	1495, 2064, 2173
TaqI	TCGA	6 cut(s)	647, 892, 1196, 1855, 2623, 3205
TatI	WGTACW	1 cut(s)	883
TfiI	GAWTC	7 cut(s)	341, 697, 1189, 1355, 1478, 1733, 1747
TscAI	CASTG	9 cut(s)	154, 190, 707, 957, 2053, 2103, 2544, 2653, 3223
TseFI	GTSAC	7 cut(s)	199, 418, 928, 1904, 2539, 2554, 2602
TseI	GCWGC	8 cut(s)	235, 525, 580, 589, 854, 1289, 3014, 3237
Tsp45I	GTSAC	7 cut(s)	199, 418, 928, 1904, 2539, 2554, 2602
TspGWI	ACGGA	5 cut(s)	437, 611, 629, 1757, 2268
TspMI	CCCGGG	1 cut(s)	2430
TspRI	CASTG	9 cut(s)	154, 190, 707, 957, 2053, 2103, 2544, 2653, 3223
Van91I	CCANNNNNTGG	1 cut(s)	1308
Vha464I	CTTAAG	1 cut(s)	122
VneI	GTGCAC	1 cut(s)	1257
VpaK11BI	GGWCC	6 cut(s)	262, 901, 1960, 2093, 2940, 3324
VspI	ATTAAT	1 cut(s)	1464
XapI	RAATTY	7 cut(s)	225, 983, 1448, 1573, 1738, 2085, 2741
XceI	RCATGY	2 cut(s)	1948, 2071
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1593, 3304
XmaI	CCCGGG	1 cut(s)	2430
ZraI	GACGTC	1 cut(s)	2062
ZrmI	AGTACT	1 cut(s)	885
Zsp2I	ATGCAT	1 cut(s)	2449

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.