Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0001776

Physical Location & Seq

Genomic Coordinates Forward (+)

26438152 .. 26443106

Gene Structure 4955 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G022460.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3357 bp

ATGGCCTCAGAATCTCCAATCAATACATCAATCGGGTCCCCTAGGCTTCGCCGTGTTAGCCAAACAAGCCATGATCTAAACAGTGACATTGGGGGTGTCGAATTCGCAACCTACACAGTCCACATACCCTCCACACCTGACAACAACCCCATGTCAGCTTCCATGGAGTACTCCACTTCTCAGAGAGTGGAGGACCAATTCGTCTCCAGCTCTCTCTTCACCGGCGGTTACAATTGCGTCACGCAAGCCCAGTTGAAGGAAAAGGTCATTGAATCACAGACCAGCTACCATCCCCAGATGACTGGGGCAAAAGGGTCTTTCTGCGCCGTGCCGGGTTGTGATGCTAAGGTGGTGACTGATGAGAGGGGACTGGATATTCTCCCTTGTGAATGTGACTACAAGATTTGTATGGATTGTTTTAGGGATGCTGTGAGAAATGGTGACCACATGTGTCCTGGCTGCAAGGAGCCTTATAAGGAGCTGGATGTGACCGAGTATGCTGTGCATAATCGAAAGCAGTATCAGCAGCAGATGTCGAAAACAGAGAGGAGGATGTCGTTGATGAAGTCGACGAAGGTGATGGGGCGCCAGAGTAGCGAGTTTGATCACAACAAGTGGTTGTTTGAGACAAAGGGGAGTTATGGATATGGAAACGCTATTTGGCCAAAGGTTGATGTGGAAGGGAGTCAAGATGGGATTGGGGGAGATCCGATGGTGTTTCATGATAAGCAATGGAGGCCTCTTACGCGCAAGTTGAATATCTCTGCTGCAATTCTCAGTCCATATAGGCTTTTAATATTCGTTCGAATGGTGGTCCTTGGATTGTTTTTGCAATGGAGAATCAGAAATCCAAATGAAGATGCTGTCTGGTTGTGGGCTATGTCAGTGGTTTGTGAAATCTGGTTTGCCATCTCATGGCTACTTGATCAGCTTCCAAAGCTCTGTCCCATCAATCGCATTACCAATCTCGATGTTTTAAAGGAGAAATTTGAAACACCGAGCCTCAGCAATCCTACTGGAAAATCTGATCTTCCAGGCATAGACATTTTTGTCTCTACAGCAGATCCAGAGAAAGAACCACCTCTTGTCACCGCAAACACAATTCTGTCGATTCTTGCAGCTGACTACCCTGTTGAGAAGCTTGCGTGTTATGTTTCTGATGATGGAGGTGCACTTTTGACATTTGAGGCCATGGCAGAAGCTGCTAGTTTTGCCAACTTGTGGGTACCTTTTTGCCGGAAGCATAATATTGAGCCAAGAAACCCAGAATCTTACTTCAGTCTGAAGAAAGATCCTTACAAGAACAAAGTACGACAAGATTTTGTTAGAGACCGAAGGAGAGTAAAGCGTGAGTATGATGAATATAAGGTTAGGATTAATGGCCTTCCTGATTCAATTCGGAGACGTTCAGAGGCCTACAATGCTAGGGATGAGATGAAGGCTATGAAACTTGGGAGAGCGATTGGAAATGATGAAGAAGTTGATAAGATAAAGATCCCAAAAGCAACTTGGATGGCTGATGGAACACACTGGCCTGGTACCTGGACAGTTCCTGCCCCTGAGCATTGTAGGGGTGATCATGCTAGTATCATGCAGGTGATGTTGAAACCTCCTAGTGATGAACCACTGAAAGGAACAACAGTGGATTCAAATTCCATGGATCTAAGTGAGGTTGACATTCGTCTTCCAATGTTAGTTTATGTTTCTCGTGAGAAGCGACCTGGCTATGATCACAACAAGAAGGCTGGTGCTATGAATGCCTTGGTTCGTGCCTCAGCTGTTATGTCCAATGGCCCCTTCATTCTCAACCTTGACTGTGACCACTACATCTACAATTCCCAAGCATTGAGAGAAGGCATGTGCTTCATGATGGATCGTGGTGGTGACCGCATTAGTTATGTCCAGTTTCCTCAAAGGTTTGAAGGAATAGACCCTTCTGACCGTTATGCCAACCACAACACTGTTTTCTTTGATGTCAACATGCGTGCACTTGATGGCATTCAAGGTCCAGTTTATGTTGGAACAGGTTGCCTCTTTCGACGAACTGCCCTTTATGGATTTGATCCACCTCGAGTGAAAGATCGTAATGGTTGTTGCTGTGGTGGCCGCAATAAGAAATCTAAATCTTCTTCAGTTGTTTCTGCCCCTGAAATTGACTCTCAAACAATACAAGTGGGAGACTTTGATGATGATAACGAAATGAATGCCATTATTCCTAAAAGGTTTGGGAACTCAAGTTTCCTTGTAGACTCTATCCAAGTGGCAGAATTCCAAGGTCGTCCTCTTGCTGATCATCCATCATTGAAAAATGGACGACCACCAGGTGCTCTTACATTTCCTCGTGAGCTTCTTGATGCATCAACAGTTGCAGAGGCAATCAGTGTCATCTCCTGCTGGTATGAGGATAAGACTGAATGGGGCCAACGCATTGGGTGGATTTATGGTTCAGTAACTGAAGATGTTGTCACAGGTTATAGGATGCATAATAGAGGATGGAGGTCTGTTTATTGTGTAACAAAAAGAGATGCATTTCGGGGTTCTGCCCCAATCAATCTCACTGATCGCCTTCACCAAGTTCTCCGATGGGCTACTGGATCTGTCGAGATTTTCTTTTCCCGAAACAATGCTCTTTTCGCAAGCTCCAAAATGAAGTTTCTACAAAGGATAGCATACCTCAATGTTGGAATGTACCCATTCACTTCCTTTTTCCTCATTGTCTACTGCTTCCTTCCAGCACTTTCACTCATTTCCGGCCAGTTCATAGTTCAGACCCTCAACATAACCTTCCTGGTATACCTTTTAGGCATCACATTGACCCTCATTCTTCTTGCTGTGCTCGAGATCAAGTGGTCTGGAATTGAACTAGAAGAGTGGTGGAGGAATGAACAGTTTTGGTTGATTGGAGGAACAAGCGCCCATCTTGCAGCTGTGCTTCAAGGTCTTCTAAAGGTCATTGCAGGTATTGAGATCTCATTCACACTGACATCAAAATCTGGAGGTGGTGATGATATAGATGATGAGTTTGTGGATTTGTATATCTTCAAATGGACATCACTGATGATACCGCCAATCACAATCATGATGACAAACTTGATTGGAATAGCTATTGCTGTTTGTCGAACAGTATATAGTGTCATACCACAGTGGAGCAAATTGCTTGGAGGGGTGTTCTTCAGTTTTTGGGTGCTGGCACATCTCTATCCCTTTGCAAAAGGGCTTATGGGAAGACGAGGGAAGACACCGACCATTGTGTTTGTGTGGTCTGGTCTTATGGCAATCACCATATCTCTCCTTTGGGTGGCTATTAGTCCCCCATCAGGTACTAATCAAATTGGTGGGTCATTCCAGTTCCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cellulose_synt zf-RING_4

Protein Analysis

1118

Amino Acids

125.8

Weight (kDa)

7.22

Isoelectric Point (pI)

41.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_4	PF14570	110 - 159	4e-17	RING/Ubox like zinc-binding domain
Cellulose_synt	PF03552	347 - 1109	0.0	Cellulose synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001776)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G02730 AT1G32180 AT1G32180
fragaria_vesca	FvH4_5g23130 FvH4_5g23130 FvH4_5g23130 FvH4_5g23130 FvH4_5g23130 FvH4_5g23130 FvH4_5g23130 FvH4_5g23160 FvH4_5g23160
prunus_persica	Prupe.5G027500_v2.0.a1 Prupe.5G027500_v2.0.a1 Prupe.5G027500_v2.0.a1 Prupe.5G027500_v2.0.a1 Prupe.5G027700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr7g0212001 RchiOBHm_Chr7g0212061
rosa_laevigata	RLG00000002942 RLG00000002946
rosa_multiflora	Rmu_co8225350.1_g000001 Rmu_co8305165.1_g000001 Rmu_co8314167.1_g000001 Rmu_co8341615.1_g000001 Rmu_co8366393.1_g000001 Rmu_co8499369.1_g000001 Rmu_sc0002283.1_g000005 Rmu_sc0002283.1_g000014 Rmu_sc0014812.1_g000004 Rmu_sc0014812.1_g000013
rosa_roxburghii	Rroxscaffold_3G00247170 Rroxscaffold_3G00247200
rosa_rugosa	Rorug07G0130000.1 Rorug07G0130100 Rorug07G0130200.1 Rorug07G0130300
rosa_samantha	Rh7AG262900 Rh7AG263400 Rh7BG257000 Rh7BG257200 Rh7CG279500 Rh7CG279900 Rh7DG269700 Rh7DG270200
rosa_wichuraiana	Rw7G022430 Rw7G022460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	474
AarI	CACCTGC	1 cut(s)	1585
AasI	GACNNNNNNGTC	2 cut(s)	200, 1049
Acc36I	ACCTGC	2 cut(s)	1585, 2948
Acc65I	GGTACC	2 cut(s)	1225, 1538
AccB1I	GGYRCC	3 cut(s)	585, 1225, 1538
AccB7I	CCANNNNNTGG	2 cut(s)	915, 1221
AccI	GTMKAC	4 cut(s)	569, 2247, 2718, 2793
AccII	CGCG	1 cut(s)	748
AciI	CCGC	5 cut(s)	225, 1092, 1888, 2107, 3065
AclWI	GGATC	8 cut(s)	701, 1058, 1286, 1489, 1668, 1881, 2057, 2602
AcoI	YGGCCR	3 cut(s)	662, 2104, 2752
AcsI	RAATTY	4 cut(s)	101, 986, 1651, 2267
AcuI	CTGAAG	5 cut(s)	1261, 1304, 2115, 2475, 3157
AcyI	GRCGYC	1 cut(s)	586
AdeI	CACNNNGTG	1 cut(s)	2324
AfaI	GTAC	6 cut(s)	170, 1227, 1311, 1540, 2690, 3322
AfiI	CCNNNNNNNGG	6 cut(s)	256, 915, 1221, 1631, 3298, 3317
AflIII	ACRYGT	1 cut(s)	447
AjnI	CCWGG	7 cut(s)	454, 1033, 1534, 1541, 1720, 2320, 2787
AjuI	GAANNNNNNNTTGG	4 cut(s)	643, 675, 1781, 1813
AloI	GAACNNNNNNTCC	2 cut(s)	2428, 2460
Alw21I	GWGCWC	4 cut(s)	1174, 1990, 2329, 2838
Alw26I	GTCTC	6 cut(s)	208, 620, 1057, 1323, 1396, 2172
Alw44I	GTGCAC	2 cut(s)	1170, 1986
AlwI	GGATC	8 cut(s)	701, 1058, 1286, 1489, 1668, 1881, 2057, 2602
AlwNI	CAGNNNCTG	3 cut(s)	1202, 1553, 2453
Ama87I	CYCGRG	2 cut(s)	2070, 2837
ApaLI	GTGCAC	2 cut(s)	1170, 1986
ApeKI	GCWGC	6 cut(s)	459, 526, 767, 1118, 1202, 2924
ApoI	RAATTY	4 cut(s)	101, 986, 1651, 2267
AseI	ATTAAT	1 cut(s)	1377
Asp718I	GGTACC	2 cut(s)	1225, 1538
AspA2I	CCTAGG	1 cut(s)	41
AspLEI	GCGC	4 cut(s)	326, 588, 750, 2915
AspS9I	GGNCC	6 cut(s)	36, 193, 814, 1793, 2006, 2419
AsuC2I	CCSGG	1 cut(s)	333
AsuII	TTCGAA	1 cut(s)	805
AvaI	CYCGRG	2 cut(s)	2070, 2837
AvaII	GGWCC	4 cut(s)	36, 193, 814, 2006
AvrII	CCTAGG	1 cut(s)	41
BaeGI	GKGCMC	2 cut(s)	1174, 1990
BalI	TGGCCA	1 cut(s)	664
BanI	GGYRCC	3 cut(s)	585, 1225, 1538
BauI	CACGAG	2 cut(s)	1707, 2340
BbsI	GAAGAC	4 cut(s)	1676, 2933, 3232, 3242
Bbv12I	GWGCWC	4 cut(s)	1174, 1990, 2329, 2838
BbvCI	CCTCAGC	2 cut(s)	1004, 1774
BbvI	GCAGC	6 cut(s)	446, 538, 754, 1130, 1189, 2936
BceAI	ACGGC	2 cut(s)	36, 311
BcgI	CGANNNNNNTGC	2 cut(s)	2186, 2220
BciT130I	CCWGG	7 cut(s)	456, 1035, 1536, 1543, 1722, 2322, 2789
BclI	TGATCA	5 cut(s)	604, 925, 1576, 1729, 2290
BcnI	CCSGG	1 cut(s)	333
BcoDI	GTCTC	6 cut(s)	208, 620, 1057, 1323, 1396, 2172
BfaI	CTAG	6 cut(s)	42, 1206, 1425, 1584, 1614, 2864
BfmI	CTRYAG	1 cut(s)	1056
BfoI	RGCGCY	2 cut(s)	589, 2916
BfuAI	ACCTGC	2 cut(s)	1585, 2948
BglII	AGATCT	1 cut(s)	2967
BisI	GCNGC	7 cut(s)	460, 527, 768, 1119, 1203, 2107, 2925
BlnI	CCTAGG	1 cut(s)	41
BlsI	GCNGC	7 cut(s)	461, 528, 769, 1120, 1204, 2108, 2926
BmcAI	AGTACT	1 cut(s)	170
Bme1390I	CCNGG	8 cut(s)	333, 456, 1035, 1536, 1543, 1722, 2322, 2789
Bme18I	GGWCC	4 cut(s)	36, 193, 814, 2006
BmeT110I	CYCGRG	2 cut(s)	2070, 2837
BmgT120I	GGNCC	6 cut(s)	36, 193, 814, 1793, 2006, 2419
BmiI	GGNNCC	8 cut(s)	37, 38, 468, 587, 1227, 1540, 1795, 2420
BmrFI	CCNGG	8 cut(s)	333, 456, 1035, 1536, 1543, 1722, 2322, 2789
BmrI	ACTGGG	2 cut(s)	244, 312
BmsI	GCATC	8 cut(s)	331, 415, 850, 2344, 2366, 2469, 2515, 2814
BmuI	ACTGGG	2 cut(s)	244, 312
BoxI	GACNNNNGTC	1 cut(s)	1680
BpiI	GAAGAC	4 cut(s)	1676, 2933, 3232, 3242
BpmI	CTGGAG	2 cut(s)	190, 3015
Bpu10I	CCTNAGC	4 cut(s)	345, 1004, 1560, 1774
Bpu14I	TTCGAA	1 cut(s)	805
BpuEI	CTTGAG	1 cut(s)	2218
BpuMI	CCSGG	1 cut(s)	333
BsaBI	GATNNNNATC	1 cut(s)	1493
BsaHI	GRCGYC	1 cut(s)	586
BsaI	GGTCTC	1 cut(s)	1323
BsaJI	CCNNGG	7 cut(s)	41, 162, 817, 1191, 1656, 1761, 2272
BsaXI	ACNNNNNCTCC	2 cut(s)	113, 143
Bsc4I	CCNNNNNNNGG	6 cut(s)	256, 915, 1221, 1631, 3298, 3317
Bse118I	RCCGGY	1 cut(s)	221
Bse3DI	GCAATG	3 cut(s)	737, 839, 2952
Bse8I	GATNNNNATC	1 cut(s)	1493
BseBI	CCWGG	7 cut(s)	456, 1035, 1536, 1543, 1722, 2322, 2789
BseDI	CCNNGG	7 cut(s)	41, 162, 817, 1191, 1656, 1761, 2272
BseGI	GGATG	9 cut(s)	289, 430, 490, 558, 1435, 1518, 2293, 2484, 2498
BseJI	GATNNNNATC	1 cut(s)	1493
BseLI	CCNNNNNNNGG	6 cut(s)	256, 915, 1221, 1631, 3298, 3317
BseMI	GCAATG	3 cut(s)	737, 839, 2952
BseMII	CTCAG	6 cut(s)	21, 194, 790, 1018, 1551, 1788
BseRI	GAGGAG	1 cut(s)	562
BseSI	GKGCMC	2 cut(s)	1174, 1990
BseXI	GCAGC	6 cut(s)	446, 538, 754, 1130, 1189, 2936
Bsh1236I	CGCG	1 cut(s)	748
BshNI	GGYRCC	3 cut(s)	585, 1225, 1538
BsiHKAI	GWGCWC	4 cut(s)	1174, 1990, 2329, 2838
BsiHKCI	CYCGRG	2 cut(s)	2070, 2837
BsiSI	CCGG	4 cut(s)	222, 332, 1237, 2751
BslFI	GGGAC	4 cut(s)	22, 381, 930, 3294
BslI	CCNNNNNNNGG	6 cut(s)	256, 915, 1221, 1631, 3298, 3317
BsmAI	GTCTC	6 cut(s)	208, 620, 1057, 1323, 1396, 2172
BsmBI	CGTCTC	2 cut(s)	208, 1396
BsmFI	GGGAC	4 cut(s)	22, 381, 930, 3294
BsmI	GAATGC	3 cut(s)	1762, 1998, 2209
Bso31I	GGTCTC	1 cut(s)	1323
BsoBI	CYCGRG	2 cut(s)	2070, 2837
Bsp119I	TTCGAA	1 cut(s)	805
Bsp1286I	GDGCHC	4 cut(s)	1174, 1990, 2329, 2838
Bsp19I	CCATGG	3 cut(s)	162, 1191, 1656
BspACI	CCGC	5 cut(s)	225, 1092, 1888, 2107, 3065
BspCNI	CTCAG	6 cut(s)	20, 193, 789, 1017, 1552, 1787
BspFNI	CGCG	1 cut(s)	748
BspHI	TCATGA	3 cut(s)	721, 1866, 3078
BspLI	GGNNCC	8 cut(s)	37, 38, 468, 587, 1227, 1540, 1795, 2420
BspMI	ACCTGC	2 cut(s)	1585, 2948
BspPI	GGATC	8 cut(s)	701, 1058, 1286, 1489, 1668, 1881, 2057, 2602
BspT104I	TTCGAA	1 cut(s)	805
BspT107I	GGYRCC	3 cut(s)	585, 1225, 1538
BspTNI	GGTCTC	1 cut(s)	1323
BsrDI	GCAATG	3 cut(s)	737, 839, 2952
BsrFI	RCCGGY	1 cut(s)	221
BssAI	RCCGGY	1 cut(s)	221
BssECI	CCNNGG	7 cut(s)	41, 162, 817, 1191, 1656, 1761, 2272
BssNAI	GTATAC	1 cut(s)	2794
BssNI	GRCGYC	1 cut(s)	586
BssSI	CACGAG	2 cut(s)	1707, 2340
BssT1I	CCWWGG	7 cut(s)	41, 162, 817, 1191, 1656, 1761, 2272
Bst1107I	GTATAC	1 cut(s)	2794
Bst2BI	CACGAG	2 cut(s)	1707, 2340
Bst2UI	CCWGG	7 cut(s)	456, 1035, 1536, 1543, 1722, 2322, 2789
Bst6I	CTCTTC	2 cut(s)	221, 2862
BstACI	GRCGYC	1 cut(s)	586
BstAPI	GCANNNNNTGC	1 cut(s)	1202
BstBI	TTCGAA	1 cut(s)	805
BstC8I	GCNNGC	5 cut(s)	246, 1143, 1986, 2638, 3189
BstDEI	CTNAG	8 cut(s)	7, 180, 345, 776, 1004, 1560, 1664, 1774
BstDSI	CCRYGG	3 cut(s)	162, 1191, 1656
BstEII	GGTNACC	2 cut(s)	440, 1883
BstF5I	GGATG	9 cut(s)	289, 430, 490, 558, 1435, 1518, 2293, 2484, 2498
BstFNI	CGCG	1 cut(s)	748
BstH2I	RGCGCY	2 cut(s)	589, 2916
BstHHI	GCGC	4 cut(s)	326, 588, 750, 2915
BstMAI	GTCTC	6 cut(s)	208, 620, 1057, 1323, 1396, 2172
BstNI	CCWGG	7 cut(s)	456, 1035, 1536, 1543, 1722, 2322, 2789
BstNSI	RCATGY	3 cut(s)	451, 1861, 1984
BstPAI	GACNNNNGTC	1 cut(s)	1680
BstPI	GGTNACC	2 cut(s)	440, 1883
BstSCI	CCNGG	8 cut(s)	331, 454, 1033, 1534, 1541, 1720, 2320, 2787
BstSFI	CTRYAG	1 cut(s)	1056
BstSLI	GKGCMC	2 cut(s)	1174, 1990
BstUI	CGCG	1 cut(s)	748
BstV1I	GCAGC	6 cut(s)	446, 538, 754, 1130, 1189, 2936
BstV2I	GAAGAC	4 cut(s)	1676, 2933, 3232, 3242
BstX2I	RGATCY	7 cut(s)	706, 1063, 1291, 1494, 1660, 2594, 2967
BstXI	CCANNNNNNTGG	2 cut(s)	302, 2429
BstYI	RGATCY	7 cut(s)	706, 1063, 1291, 1494, 1660, 2594, 2967
BstZ17I	GTATAC	1 cut(s)	2794
BtgI	CCRYGG	3 cut(s)	162, 1191, 1656
BtsCI	GGATG	9 cut(s)	289, 430, 490, 558, 1435, 1518, 2293, 2484, 2498
BveI	ACCTGC	2 cut(s)	1585, 2948
Cac8I	GCNNGC	5 cut(s)	246, 1143, 1986, 2638, 3189
CaiI	CAGNNNCTG	3 cut(s)	1202, 1553, 2453
CciI	TCATGA	3 cut(s)	721, 1866, 3078
CfoI	GCGC	4 cut(s)	326, 588, 750, 2915
Cfr10I	RCCGGY	1 cut(s)	221
Cfr13I	GGNCC	6 cut(s)	36, 193, 814, 1793, 2006, 2419
CseI	GACGC	1 cut(s)	226
CsiI	ACCWGGT	1 cut(s)	2320
Csp6I	GTAC	6 cut(s)	169, 1226, 1310, 1539, 2689, 3321
CspCI	CAANNNNNGTGG	2 cut(s)	2154, 2189
CviQI	GTAC	6 cut(s)	169, 1226, 1310, 1539, 2689, 3321
DdeI	CTNAG	8 cut(s)	7, 180, 345, 776, 1004, 1560, 1664, 1774
DinI	GGCGCC	1 cut(s)	587
DraI	TTTAAA	1 cut(s)	978
DraIII	CACNNNGTG	1 cut(s)	2324
DrdI	GACNNNNNNGTC	2 cut(s)	200, 1049
DseDI	GACNNNNNNGTC	2 cut(s)	200, 1049
EaeI	YGGCCR	3 cut(s)	662, 2104, 2752
Eam1104I	CTCTTC	2 cut(s)	221, 2862
EarI	CTCTTC	2 cut(s)	221, 2862
Eco130I	CCWWGG	7 cut(s)	41, 162, 817, 1191, 1656, 1761, 2272
Eco147I	AGGCCT	2 cut(s)	739, 1415
Eco31I	GGTCTC	1 cut(s)	1323
Eco47I	GGWCC	4 cut(s)	36, 193, 814, 2006
Eco57I	CTGAAG	5 cut(s)	1261, 1304, 2115, 2475, 3157
Eco88I	CYCGRG	2 cut(s)	2070, 2837
Eco91I	GGTNACC	2 cut(s)	440, 1883
EcoO109I	RGGNCCY	1 cut(s)	36
EcoO65I	GGTNACC	2 cut(s)	440, 1883
EcoRI	GAATTC	2 cut(s)	101, 2267
EcoRII	CCWGG	7 cut(s)	454, 1033, 1534, 1541, 1720, 2320, 2787
EcoT14I	CCWWGG	7 cut(s)	41, 162, 817, 1191, 1656, 1761, 2272
EcoT22I	ATGCAT	3 cut(s)	2359, 2484, 2530
EgeI	GGCGCC	1 cut(s)	587
EheI	GGCGCC	1 cut(s)	587
ErhI	CCWWGG	7 cut(s)	41, 162, 817, 1191, 1656, 1761, 2272
Esp3I	CGTCTC	2 cut(s)	208, 1396
FaqI	GGGAC	4 cut(s)	22, 381, 930, 3294
FbaI	TGATCA	5 cut(s)	604, 925, 1576, 1729, 2290
FblI	GTMKAC	4 cut(s)	569, 2247, 2718, 2793
Fnu4HI	GCNGC	7 cut(s)	460, 527, 768, 1119, 1203, 2107, 2925
FokI	GGATG	9 cut(s)	276, 437, 497, 565, 1442, 1525, 2280, 2491, 2505
Fsp4HI	GCNGC	7 cut(s)	460, 527, 768, 1119, 1203, 2107, 2925
FspBI	CTAG	6 cut(s)	42, 1206, 1425, 1584, 1614, 2864
GlaI	GCGC	4 cut(s)	325, 587, 749, 2914
GluI	GCNGC	7 cut(s)	460, 527, 768, 1119, 1203, 2107, 2925
GsuI	CTGGAG	2 cut(s)	190, 3015
HaeII	RGCGCY	2 cut(s)	589, 2916
HapII	CCGG	4 cut(s)	222, 332, 1237, 2751
HgaI	GACGC	1 cut(s)	226
HhaI	GCGC	4 cut(s)	326, 588, 750, 2915
Hin1I	GRCGYC	1 cut(s)	586
Hin6I	GCGC	4 cut(s)	324, 586, 748, 2913
HinP1I	GCGC	4 cut(s)	324, 586, 748, 2913
HincII	GTYRAC	3 cut(s)	570, 1675, 1978
HindII	GTYRAC	3 cut(s)	570, 1675, 1978
HindIII	AAGCTT	1 cut(s)	1139
HpaII	CCGG	4 cut(s)	222, 332, 1237, 2751
Hpy166II	GTNNAC	9 cut(s)	121, 570, 1172, 1675, 1978, 1988, 2248, 2719, 2794
Hpy8I	GTNNAC	9 cut(s)	121, 570, 1172, 1675, 1978, 1988, 2248, 2719, 2794
Hpy99I	CGWCG	2 cut(s)	574, 2043
HpyCH4IV	ACGT	1 cut(s)	1405
HpyF3I	CTNAG	8 cut(s)	7, 180, 345, 776, 1004, 1560, 1664, 1774
HpySE526I	ACGT	1 cut(s)	1405
Hsp92I	GRCGYC	1 cut(s)	586
HspAI	GCGC	4 cut(s)	324, 586, 748, 2913
KasI	GGCGCC	1 cut(s)	585
KflI	GGGWCCC	1 cut(s)	36
KpnI	GGTACC	2 cut(s)	1229, 1542
Ksp22I	TGATCA	5 cut(s)	604, 925, 1576, 1729, 2290
LmnI	GCTCC	4 cut(s)	466, 478, 2645, 3147
Lsp1109I	GCAGC	6 cut(s)	446, 538, 754, 1130, 1189, 2936
LweI	GCATC	8 cut(s)	331, 415, 850, 2344, 2366, 2469, 2515, 2814
MabI	ACCWGGT	1 cut(s)	2320
MaeI	CTAG	6 cut(s)	42, 1206, 1425, 1584, 1614, 2864
MaeII	ACGT	1 cut(s)	1405
MfeI	CAATTG	1 cut(s)	232
MflI	RGATCY	7 cut(s)	706, 1063, 1291, 1494, 1660, 2594, 2967
MhlI	GDGCHC	4 cut(s)	1174, 1990, 2329, 2838
MlsI	TGGCCA	1 cut(s)	664
MluNI	TGGCCA	1 cut(s)	664
Mly113I	GGCGCC	1 cut(s)	586
MlyI	GAGTC	3 cut(s)	694, 2150, 2243
MmeI	TCCRAC	2 cut(s)	1999, 2662
Mox20I	TGGCCA	1 cut(s)	664
Mph1103I	ATGCAT	3 cut(s)	2359, 2484, 2530
MscI	TGGCCA	1 cut(s)	664
MseI	TTAA	3 cut(s)	794, 977, 1377
MslI	CAYNNNNRTG	2 cut(s)	1595, 3077
Msp20I	TGGCCA	1 cut(s)	664
MspA1I	CMGCKG	3 cut(s)	1121, 1778, 2927
MspI	CCGG	4 cut(s)	222, 332, 1237, 2751
MspR9I	CCNGG	8 cut(s)	333, 456, 1035, 1536, 1543, 1722, 2322, 2789
MunI	CAATTG	1 cut(s)	232
Mva1269I	GAATGC	3 cut(s)	1762, 1998, 2209
MvaI	CCWGG	7 cut(s)	456, 1035, 1536, 1543, 1722, 2322, 2789
MvnI	CGCG	1 cut(s)	748
NarI	GGCGCC	1 cut(s)	586
NciI	CCSGG	1 cut(s)	333
NcoI	CCATGG	3 cut(s)	162, 1191, 1656
NlaIV	GGNNCC	8 cut(s)	37, 38, 468, 587, 1227, 1540, 1795, 2420
NsiI	ATGCAT	3 cut(s)	2359, 2484, 2530
NspI	RCATGY	3 cut(s)	451, 1861, 1984
NspV	TTCGAA	1 cut(s)	805
PaeR7I	CTCGAG	2 cut(s)	2070, 2837
PagI	TCATGA	3 cut(s)	721, 1866, 3078
PaqCI	CACCTGC	1 cut(s)	1585
PceI	AGGCCT	2 cut(s)	739, 1415
PciI	ACATGT	1 cut(s)	447
PcsI	WCGNNNNNNNCGW	1 cut(s)	1714
PctI	GAATGC	3 cut(s)	1762, 1998, 2209
PfeI	GAWTC	7 cut(s)	11, 272, 840, 1111, 1268, 1391, 1646
PflMI	CCANNNNNTGG	2 cut(s)	915, 1221
PkrI	GCNGC	7 cut(s)	461, 528, 769, 1120, 1204, 2108, 2926
PleI	GAGTC	3 cut(s)	693, 2150, 2243
PluTI	GGCGCC	1 cut(s)	589
PpsI	GAGTC	3 cut(s)	693, 2150, 2243
PpuMI	RGGWCCY	1 cut(s)	36
PscI	ACATGT	1 cut(s)	447
PshAI	GACNNNNGTC	1 cut(s)	1680
PshBI	ATTAAT	1 cut(s)	1377
PsiI	TTATAA	1 cut(s)	474
Psp5II	RGGWCCY	1 cut(s)	36
Psp6I	CCWGG	7 cut(s)	454, 1033, 1534, 1541, 1720, 2320, 2787
PspEI	GGTNACC	2 cut(s)	440, 1883
PspGI	CCWGG	7 cut(s)	454, 1033, 1534, 1541, 1720, 2320, 2787
PspN4I	GGNNCC	8 cut(s)	37, 38, 468, 587, 1227, 1540, 1795, 2420
PspPI	GGNCC	6 cut(s)	36, 193, 814, 1793, 2006, 2419
PspPPI	RGGWCCY	1 cut(s)	36
PspXI	VCTCGAGB	1 cut(s)	2070
PstNI	CAGNNNCTG	3 cut(s)	1202, 1553, 2453
PsuI	RGATCY	7 cut(s)	706, 1063, 1291, 1494, 1660, 2594, 2967
PvuII	CAGCTG	3 cut(s)	1121, 1778, 2927
RsaI	GTAC	6 cut(s)	170, 1227, 1311, 1540, 2690, 3322
RsaNI	GTAC	6 cut(s)	169, 1226, 1310, 1539, 2689, 3321
RseI	CAYNNNNRTG	2 cut(s)	1595, 3077
SalI	GTCGAC	1 cut(s)	568
SaqAI	TTAA	3 cut(s)	794, 977, 1377
SatI	GCNGC	7 cut(s)	460, 527, 768, 1119, 1203, 2107, 2925
Sau96I	GGNCC	6 cut(s)	36, 193, 814, 1793, 2006, 2419
ScaI	AGTACT	1 cut(s)	170
SchI	GAGTC	3 cut(s)	694, 2150, 2243
ScrFI	CCNGG	8 cut(s)	333, 456, 1035, 1536, 1543, 1722, 2322, 2789
SduI	GDGCHC	4 cut(s)	1174, 1990, 2329, 2838
SexAI	ACCWGGT	1 cut(s)	2320
SfaNI	GCATC	8 cut(s)	331, 415, 850, 2344, 2366, 2469, 2515, 2814
SfcI	CTRYAG	1 cut(s)	1056
SfoI	GGCGCC	1 cut(s)	587
Sfr274I	CTCGAG	2 cut(s)	2070, 2837
SfuI	TTCGAA	1 cut(s)	805
SgrAI	CRCCGGYG	1 cut(s)	221
SinI	GGWCC	4 cut(s)	36, 193, 814, 2006
SlaI	CTCGAG	2 cut(s)	2070, 2837
SmiMI	CAYNNNNRTG	2 cut(s)	1595, 3077
SmlI	CTYRAG	3 cut(s)	2070, 2233, 2837
SmoI	CTYRAG	3 cut(s)	2070, 2233, 2837
SseBI	AGGCCT	2 cut(s)	739, 1415
SsiI	CCGC	5 cut(s)	225, 1092, 1888, 2107, 3065
SspDI	GGCGCC	1 cut(s)	585
SspI	AATATT	2 cut(s)	798, 1249
SspMI	CTAG	6 cut(s)	42, 1206, 1425, 1584, 1614, 2864
StuI	AGGCCT	2 cut(s)	739, 1415
StyD4I	CCNGG	8 cut(s)	331, 454, 1033, 1534, 1541, 1720, 2320, 2787
StyI	CCWWGG	7 cut(s)	41, 162, 817, 1191, 1656, 1761, 2272
TaiI	ACGT	1 cut(s)	1408
TaqII	GACCGA	2 cut(s)	506, 1347
TatI	WGTACW	1 cut(s)	168
TauI	GCSGC	1 cut(s)	2109
TfiI	GAWTC	7 cut(s)	11, 272, 840, 1111, 1268, 1391, 1646
Tru1I	TTAA	3 cut(s)	794, 977, 1377
Tru9I	TTAA	3 cut(s)	794, 977, 1377
TseI	GCWGC	6 cut(s)	459, 526, 767, 1118, 1202, 2924
Van91I	CCANNNNNTGG	2 cut(s)	915, 1221
VneI	GTGCAC	2 cut(s)	1170, 1986
VpaK11BI	GGWCC	4 cut(s)	36, 193, 814, 2006
VspI	ATTAAT	1 cut(s)	1377
XapI	RAATTY	4 cut(s)	101, 986, 1651, 2267
XceI	RCATGY	3 cut(s)	451, 1861, 1984
XcmI	CCANNNNNNNNNTGG	2 cut(s)	452, 1506
XhoI	CTCGAG	2 cut(s)	2070, 2837
XmaJI	CCTAGG	1 cut(s)	41
XmiI	GTMKAC	4 cut(s)	569, 2247, 2718, 2793
XspI	CTAG	6 cut(s)	42, 1206, 1425, 1584, 1614, 2864
ZrmI	AGTACT	1 cut(s)	170
Zsp2I	ATGCAT	3 cut(s)	2359, 2484, 2530

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.