FvH4_5g23820

Transmembrane protein 45B-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0008026
Physical Location & Seq
Forward (+)
15110411 .. 15115877
5467 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g23820.t1

Sequence Viewer

Length: 852 bp
ATGGGTTCTTTTGGTGGTCATGCTGTGCCAGGGACGTTGTTTCTTGTAGTTGGGTTGTGGCATATATGGAGCGCGTTGGTCAGATATGTATCCGACCCAAAGTCCTTTCGGGTCCGGGTTTGGAGTCCTGTGCCGGGTTTTGATGGGAGGATCAAGTACCTGGAGCTTTATGTTATAGCCATTGGTTCATTTATTGATCTGTGTATCGAGCTTTTCTATTCGCCCCATTTCAAGTATTTTGTTAATGGAGTTTTGAACCCTGCTCACATGAATGATTTTGAGCACTCGGGGATGCTTCTCATGTTCTTTATCTTCGGCTTAATTGCTCTGCTGTCAGAGAAGACAAGATTTCTTCCGTTGCCTGAAGGAGCTCTTTGTCTGATTGCTGCCACAGCCTTCTCTGTGGAGTATCTCCTGTTCTACTTTCACTCAACAACTCATAAAGGCCTCGAGGGGTATTACCACATCATCCTTGTTCTTCTGATTGGGCTCTGTATATTATCAACTGTTACAGGAGCACTCCTACCAACCAGCTTTCCAGTTGATTTATGCGGTGGTATTGCCCTAACTCTCCAAGGCATCTGGTTTTATCAGACTGCATTCACCCTCTATGGTCCAATGATGCCAGATGGTTGTCAGTTGAAGAGTGATACGGTCTCATGTCATTCTGCAGACAGTGAGGTCCGGGGAGAGTTACTTGCAAACTTCCAGATCTTTTCCATAGTTCTTGGTGTACTTGTTGCAGTTGTGGTAGCATATGGTTTTGTTGCTTCAAGATTTGGGCATTCAGAAGTTAGGCGTGTGCATGTAGTACAAGATGTATTGGTTGACGATCAAATTAGGGGTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF716

Protein Analysis

284

Amino Acids

31.4

Weight (kDa)

6.09

Isoelectric Point (pI)

34.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF716 PF04819 123 - 254 3.2e-44 Family of unknown function (DUF716)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 680
AbsI CCTCGAGG 1 cut(s) 449
AccII CGCG 1 cut(s) 74
AciI CCGC 1 cut(s) 552
AclWI GGATC 1 cut(s) 158
AcuI CTGAAG 1 cut(s) 384
AfaI GTAC 3 cut(s) 158, 735, 813
AfiI CCNNNNNNNGG 1 cut(s) 134
AgsI TTSAA 4 cut(s) 232, 256, 643, 774
AhdI GACNNNNNGTC 1 cut(s) 100
AjnI CCWGG 2 cut(s) 28, 159
AluBI AGCT 4 cut(s) 166, 211, 371, 534
AluI AGCT 4 cut(s) 166, 211, 371, 534
Alw21I GWGCWC 3 cut(s) 285, 373, 520
Alw26I GTCTC 1 cut(s) 661
AlwI GGATC 1 cut(s) 158
Ama87I CYCGRG 2 cut(s) 286, 449
AoxI GGCC 1 cut(s) 445
ApeKI GCWGC 1 cut(s) 386
AspLEI GCGC 1 cut(s) 74
AspS9I GGNCC 3 cut(s) 112, 614, 682
AsuC2I CCSGG 3 cut(s) 116, 135, 686
AsuHPI GGTGA 1 cut(s) 595
AvaI CYCGRG 2 cut(s) 286, 449
AvaII GGWCC 3 cut(s) 112, 614, 682
BanII GRGCYC 2 cut(s) 373, 492
BbsI GAAGAC 1 cut(s) 347
Bbv12I GWGCWC 3 cut(s) 285, 373, 520
BbvI GCAGC 1 cut(s) 373
BccI CCATC 2 cut(s) 137, 623
BciT130I CCWGG 2 cut(s) 30, 161
BciVI GTATCC 1 cut(s) 100
BcnI CCSGG 3 cut(s) 116, 135, 686
BcoDI GTCTC 1 cut(s) 661
BfaI CTAG 1 cut(s) 850
BfmI CTRYAG 1 cut(s) 669
BfuI GTATCC 1 cut(s) 100
BglII AGATCT 1 cut(s) 711
BisI GCNGC 1 cut(s) 387
BlsI GCNGC 1 cut(s) 388
Bme1390I CCNGG 5 cut(s) 30, 116, 135, 161, 686
Bme18I GGWCC 3 cut(s) 112, 614, 682
BmeRI GACNNNNNGTC 1 cut(s) 100
BmeT110I CYCGRG 2 cut(s) 286, 449
BmgT120I GGNCC 3 cut(s) 112, 614, 682
BmiI GGNNCC 1 cut(s) 113
BmrFI CCNGG 5 cut(s) 30, 116, 135, 161, 686
BmsI GCATC 3 cut(s) 282, 588, 612
BpiI GAAGAC 1 cut(s) 347
BpmI CTGGAG 1 cut(s) 182
BpuMI CCSGG 3 cut(s) 116, 135, 686
BsaBI GATNNNNATC 1 cut(s) 88
BsaI GGTCTC 1 cut(s) 661
BsaJI CCNNGG 3 cut(s) 29, 574, 685
Bsc4I CCNNNNNNNGG 1 cut(s) 134
Bse1I ACTGG 1 cut(s) 539
Bse8I GATNNNNATC 1 cut(s) 88
BseBI CCWGG 2 cut(s) 30, 161
BseDI CCNNGG 3 cut(s) 29, 574, 685
BseGI GGATG 2 cut(s) 297, 468
BseJI GATNNNNATC 1 cut(s) 88
BseLI CCNNNNNNNGG 1 cut(s) 134
BseNI ACTGG 1 cut(s) 539
BseXI GCAGC 1 cut(s) 373
Bsh1236I CGCG 1 cut(s) 74
BshFI GGCC 1 cut(s) 447
BsiHKAI GWGCWC 3 cut(s) 285, 373, 520
BsiHKCI CYCGRG 2 cut(s) 286, 449
BsiSI CCGG 3 cut(s) 115, 134, 685
BslFI GGGAC 1 cut(s) 46
BslI CCNNNNNNNGG 1 cut(s) 134
BsmAI GTCTC 1 cut(s) 661
BsmFI GGGAC 1 cut(s) 46
BsmI GAATGC 2 cut(s) 599, 784
BsnI GGCC 1 cut(s) 447
Bso31I GGTCTC 1 cut(s) 661
BsoBI CYCGRG 2 cut(s) 286, 449
Bsp1286I GDGCHC 4 cut(s) 285, 373, 492, 520
Bsp143I GATC 4 cut(s) 150, 196, 711, 832
BspACI CCGC 1 cut(s) 552
BspANI GGCC 1 cut(s) 447
BspFNI CGCG 1 cut(s) 74
BspLI GGNNCC 1 cut(s) 113
BspMAI CTGCAG 1 cut(s) 673
BspPI GGATC 1 cut(s) 158
BspTNI GGTCTC 1 cut(s) 661
BsrI ACTGG 1 cut(s) 539
BssECI CCNNGG 3 cut(s) 29, 574, 685
BssMI GATC 4 cut(s) 150, 196, 711, 832
BssT1I CCWWGG 1 cut(s) 574
Bst2UI CCWGG 2 cut(s) 30, 161
Bst4CI ACNGT 3 cut(s) 508, 655, 677
Bst6I CTCTTC 1 cut(s) 638
BstF5I GGATG 2 cut(s) 297, 468
BstFNI CGCG 1 cut(s) 74
BstHHI GCGC 1 cut(s) 74
BstKTI GATC 4 cut(s) 153, 199, 714, 835
BstMAI GTCTC 1 cut(s) 661
BstMBI GATC 4 cut(s) 150, 196, 711, 832
BstMWI GCNNNNNNNGC 1 cut(s) 392
BstNI CCWGG 2 cut(s) 30, 161
BstNSI RCATGY 1 cut(s) 809
BstSCI CCNGG 5 cut(s) 28, 114, 133, 159, 684
BstSFI CTRYAG 1 cut(s) 669
BstUI CGCG 1 cut(s) 74
BstV1I GCAGC 1 cut(s) 373
BstV2I GAAGAC 1 cut(s) 347
BstX2I RGATCY 1 cut(s) 711
BstYI RGATCY 1 cut(s) 711
BsuI GTATCC 1 cut(s) 100
BsuRI GGCC 1 cut(s) 447
BtsCI GGATG 2 cut(s) 297, 468
BtsIMutI CAGTG 1 cut(s) 682
CfoI GCGC 1 cut(s) 74
Cfr13I GGNCC 3 cut(s) 112, 614, 682
Csp6I GTAC 3 cut(s) 157, 734, 812
CviAII CATG 5 cut(s) 20, 268, 301, 660, 806
CviJI RGCY 9 cut(s) 166, 179, 211, 318, 371, 395, 447, 490, 534
CviKI_1 RGCY 9 cut(s) 166, 179, 211, 318, 371, 395, 447, 490, 534
CviQI GTAC 3 cut(s) 157, 734, 812
DpnI GATC 4 cut(s) 152, 198, 713, 834
DpnII GATC 4 cut(s) 150, 196, 711, 832
DrdI GACNNNNNNGTC 1 cut(s) 680
DriI GACNNNNNGTC 1 cut(s) 100
DseDI GACNNNNNNGTC 1 cut(s) 680
Eam1104I CTCTTC 1 cut(s) 638
Eam1105I GACNNNNNGTC 1 cut(s) 100
EarI CTCTTC 1 cut(s) 638
Ecl136II GAGCTC 1 cut(s) 371
Eco130I CCWWGG 1 cut(s) 574
Eco147I AGGCCT 1 cut(s) 447
Eco24I GRGCYC 2 cut(s) 373, 492
Eco31I GGTCTC 1 cut(s) 661
Eco47I GGWCC 3 cut(s) 112, 614, 682
Eco53kI GAGCTC 1 cut(s) 371
Eco57I CTGAAG 1 cut(s) 384
Eco88I CYCGRG 2 cut(s) 286, 449
EcoICRI GAGCTC 1 cut(s) 371
EcoRII CCWGG 2 cut(s) 28, 159
EcoT14I CCWWGG 1 cut(s) 574
EcoT38I GRGCYC 2 cut(s) 373, 492
ErhI CCWWGG 1 cut(s) 574
FaeI CATG 5 cut(s) 23, 271, 304, 663, 809
FalI AAGNNNNNCTT 2 cut(s) 357, 389
FaqI GGGAC 1 cut(s) 46
FatI CATG 5 cut(s) 19, 267, 300, 659, 805
FauNDI CATATG 1 cut(s) 757
Fnu4HI GCNGC 1 cut(s) 387
FokI GGATG 2 cut(s) 304, 455
FriOI GRGCYC 2 cut(s) 373, 492
Fsp4HI GCNGC 1 cut(s) 387
FspBI CTAG 1 cut(s) 850
GlaI GCGC 1 cut(s) 73
GluI GCNGC 1 cut(s) 387
GsuI CTGGAG 1 cut(s) 182
HaeIII GGCC 1 cut(s) 447
HapII CCGG 3 cut(s) 115, 134, 685
HhaI GCGC 1 cut(s) 74
Hin1II CATG 5 cut(s) 23, 271, 304, 663, 809
Hin6I GCGC 1 cut(s) 72
HinP1I GCGC 1 cut(s) 72
HincII GTYRAC 1 cut(s) 829
HindII GTYRAC 1 cut(s) 829
HinfI GANTC 1 cut(s) 124
HpaII CCGG 3 cut(s) 115, 134, 685
HphI GGTGA 1 cut(s) 595
Hpy166II GTNNAC 2 cut(s) 734, 829
Hpy188I TCNGA 7 cut(s) 83, 94, 337, 381, 483, 594, 790
Hpy188III TCNNGA 2 cut(s) 709, 774
Hpy8I GTNNAC 2 cut(s) 734, 829
HpyAV CCTTC 2 cut(s) 359, 406
HpyCH4III ACNGT 3 cut(s) 508, 655, 677
HpyCH4IV ACGT 1 cut(s) 35
HpyCH4V TGCA 5 cut(s) 599, 671, 701, 743, 805
HpyF10VI GCNNNNNNNGC 1 cut(s) 392
HpySE526I ACGT 1 cut(s) 35
Hsp92II CATG 5 cut(s) 23, 271, 304, 663, 809
HspAI GCGC 1 cut(s) 72
Kzo9I GATC 4 cut(s) 150, 196, 711, 832
LmnI GCTCC 4 cut(s) 69, 163, 368, 515
Lsp1109I GCAGC 1 cut(s) 373
LweI GCATC 3 cut(s) 282, 588, 612
MaeI CTAG 1 cut(s) 850
MaeII ACGT 1 cut(s) 35
MaeIII GTNAC 2 cut(s) 508, 693
MalI GATC 4 cut(s) 152, 198, 713, 834
MboI GATC 4 cut(s) 150, 196, 711, 832
MboII GAAGA 5 cut(s) 304, 344, 352, 470, 655
MflI RGATCY 1 cut(s) 711
MhlI GDGCHC 4 cut(s) 285, 373, 492, 520
MluCI AATT 2 cut(s) 321, 837
MlyI GAGTC 1 cut(s) 133
MmeI TCCRAC 1 cut(s) 117
MnlI CCTC 5 cut(s) 141, 445, 458, 617, 673
MseI TTAA 2 cut(s) 243, 320
MslI CAYNNNNRTG 1 cut(s) 270
MspI CCGG 3 cut(s) 115, 134, 685
MspR9I CCNGG 5 cut(s) 30, 116, 135, 161, 686
Mva1269I GAATGC 2 cut(s) 599, 784
MvaI CCWGG 2 cut(s) 30, 161
MvnI CGCG 1 cut(s) 74
MwoI GCNNNNNNNGC 1 cut(s) 392
NciI CCSGG 3 cut(s) 116, 135, 686
NdeI CATATG 1 cut(s) 757
NdeII GATC 4 cut(s) 150, 196, 711, 832
NlaIII CATG 5 cut(s) 23, 271, 304, 663, 809
NlaIV GGNNCC 1 cut(s) 113
NspI RCATGY 1 cut(s) 809
PaeR7I CTCGAG 1 cut(s) 449
PceI AGGCCT 1 cut(s) 447
PctI GAATGC 2 cut(s) 599, 784
PkrI GCNGC 1 cut(s) 388
PleI GAGTC 1 cut(s) 132
PpsI GAGTC 1 cut(s) 132
Psp124BI GAGCTC 1 cut(s) 373
Psp6I CCWGG 2 cut(s) 28, 159
PspGI CCWGG 2 cut(s) 28, 159
PspN4I GGNNCC 1 cut(s) 113
PspPI GGNCC 3 cut(s) 112, 614, 682
PspXI VCTCGAGB 1 cut(s) 449
PsrI GAACNNNNNNTAC 2 cut(s) 401, 433
PstI CTGCAG 1 cut(s) 673
PsuI RGATCY 1 cut(s) 711
RsaI GTAC 3 cut(s) 158, 735, 813
RsaNI GTAC 3 cut(s) 157, 734, 812
RseI CAYNNNNRTG 1 cut(s) 270
SacI GAGCTC 1 cut(s) 373
SaqAI TTAA 2 cut(s) 243, 320
SatI GCNGC 1 cut(s) 387
Sau3AI GATC 4 cut(s) 150, 196, 711, 832
Sau96I GGNCC 3 cut(s) 112, 614, 682
SchI GAGTC 1 cut(s) 133
ScrFI CCNGG 5 cut(s) 30, 116, 135, 161, 686
SduI GDGCHC 4 cut(s) 285, 373, 492, 520
SetI ASST 7 cut(s) 38, 162, 168, 213, 373, 536, 684
SfaNI GCATC 3 cut(s) 282, 588, 612
SfcI CTRYAG 1 cut(s) 669
Sfr274I CTCGAG 1 cut(s) 449
SinI GGWCC 3 cut(s) 112, 614, 682
SlaI CTCGAG 1 cut(s) 449
SmiMI CAYNNNNRTG 1 cut(s) 270
SmlI CTYRAG 1 cut(s) 449
SmoI CTYRAG 1 cut(s) 449
Sse9I AATT 2 cut(s) 321, 837
SseBI AGGCCT 1 cut(s) 447
SsiI CCGC 1 cut(s) 552
SspMI CTAG 1 cut(s) 850
SstI GAGCTC 1 cut(s) 373
StuI AGGCCT 1 cut(s) 447
StyD4I CCNGG 5 cut(s) 28, 114, 133, 159, 684
StyI CCWWGG 1 cut(s) 574
TaaI ACNGT 3 cut(s) 508, 655, 677
TaiI ACGT 1 cut(s) 38
TaqI TCGA 2 cut(s) 207, 450
TasI AATT 2 cut(s) 321, 837
TatI WGTACW 2 cut(s) 733, 811
Tru1I TTAA 2 cut(s) 243, 320
Tru9I TTAA 2 cut(s) 243, 320
TscAI CASTG 1 cut(s) 682
TseI GCWGC 1 cut(s) 386
TspDTI ATGAA 2 cut(s) 177, 284
TspGWI ACGGA 1 cut(s) 345
TspRI CASTG 1 cut(s) 682
VpaK11BI GGWCC 3 cut(s) 112, 614, 682
XceI RCATGY 1 cut(s) 809
XhoI CTCGAG 1 cut(s) 449
XspI CTAG 1 cut(s) 850
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.