Rh7DG280000

Transmembrane protein 45B-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0008026
Physical Location & Seq
Forward (+)
30226943 .. 30232437
5495 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG280000.1

Sequence Viewer

Length: 849 bp
ATGGGTTCTTTCAAGGGTCATGCTGTGCCAGGGACGTTGTTTCTTGTAGTTGGGTTGTGGCATATATGGAGCGCGTTGGTCAGATATGTATCCGACCCCAAGTCATTTCGGGTCCGGGTTTGGAATCCTGTGCCGGGTTTTGATGGGAGGATCAAGTACTTGGAGCTTTATGTTATAGCCGTTGGTGCTTTTATTGATCTGTGTATCGAGCTTTTGTATTCGCCTCATCTCAAGTTCTTTGTTAATGGAGTTTTGAACCCTGCTCACATGAATGATTTTGAGCACTCGGGGATGCTTCTTATGTTCTTTATCTTCGGTGTAATTGCTCTGCTCTCACAGGAGACAAGATTTCTTCCATTGCCTGAAGGAGCTCTTTGTCTGATTGCTGCCACGGCATTCTCTGCGGAGTATCTCTTGTTCTACTTTCACTCAACAACTCATAAAGGCCTCGAGGGGTATTACCACATCATCCTTGTCCTTCTAATTGGGCTCTGTATATTATCAACTGTTACAGGAGCGCTCCTACCAACCAGCTTTCCAGTTGATTTATGCAATGGTATTGCCTTAACTCTCCAAGGCATCTGGTTTTATCAGACTGCATTCACCCTCTATGGTCCGATGATGCCAGATGGTTGTCAGTTGAAGGAAGGAATGATCTCATGTCATTCTGCAGACAATGAGGTCCGGGGAGAGTTACTTGCAAACTTCCAGTTCTTCTCCATAGTTCTTGGTGTACTTGTTGCAGTTGTGGGAGCATATGGTTTCATTGCTTCAAGATTTGGGCATTCAGATCTTACCCGTTTGCATGCAGTACCTGAATTGGTTGACGATCAAATGAGGGGTTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF716

Protein Analysis

282

Amino Acids

31.26

Weight (kDa)

5.96

Isoelectric Point (pI)

26.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF716 PF04819 123 - 254 9.7e-46 Family of unknown function (DUF716)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 680
AbsI CCTCGAGG 1 cut(s) 449
AccII CGCG 1 cut(s) 74
AciI CCGC 1 cut(s) 404
AclWI GGATC 1 cut(s) 158
AcuI CTGAAG 1 cut(s) 384
AfaI GTAC 3 cut(s) 158, 735, 813
AfeI AGCGCT 1 cut(s) 519
AfiI CCNNNNNNNGG 1 cut(s) 134
AgsI TTSAA 4 cut(s) 13, 256, 643, 774
AhdI GACNNNNNGTC 1 cut(s) 100
AjnI CCWGG 1 cut(s) 28
AluBI AGCT 4 cut(s) 166, 211, 371, 534
AluI AGCT 4 cut(s) 166, 211, 371, 534
Alw21I GWGCWC 2 cut(s) 285, 373
Alw26I GTCTC 1 cut(s) 335
AlwI GGATC 1 cut(s) 158
AlwNI CAGNNNCTG 1 cut(s) 815
Ama87I CYCGRG 2 cut(s) 286, 449
Aor51HI AGCGCT 1 cut(s) 519
AoxI GGCC 1 cut(s) 445
ApeKI GCWGC 1 cut(s) 386
AspLEI GCGC 2 cut(s) 74, 520
AspS9I GGNCC 3 cut(s) 112, 614, 682
AsuC2I CCSGG 3 cut(s) 116, 135, 686
AsuHPI GGTGA 1 cut(s) 595
AvaI CYCGRG 2 cut(s) 286, 449
AvaII GGWCC 3 cut(s) 112, 614, 682
BanII GRGCYC 2 cut(s) 373, 492
Bbv12I GWGCWC 2 cut(s) 285, 373
BbvI GCAGC 1 cut(s) 373
BccI CCATC 2 cut(s) 137, 623
BceAI ACGGC 2 cut(s) 164, 408
BciT130I CCWGG 1 cut(s) 30
BciVI GTATCC 1 cut(s) 100
BcnI CCSGG 3 cut(s) 116, 135, 686
BcoDI GTCTC 1 cut(s) 335
BfmI CTRYAG 1 cut(s) 669
BfoI RGCGCY 1 cut(s) 521
BfuI GTATCC 1 cut(s) 100
BglII AGATCT 1 cut(s) 790
BisI GCNGC 1 cut(s) 387
BlsI GCNGC 1 cut(s) 388
BmcAI AGTACT 1 cut(s) 158
Bme1390I CCNGG 4 cut(s) 30, 116, 135, 686
Bme18I GGWCC 3 cut(s) 112, 614, 682
BmeRI GACNNNNNGTC 1 cut(s) 100
BmeT110I CYCGRG 2 cut(s) 286, 449
BmgT120I GGNCC 3 cut(s) 112, 614, 682
BmiI GGNNCC 1 cut(s) 113
BmrFI CCNGG 4 cut(s) 30, 116, 135, 686
BmsI GCATC 3 cut(s) 282, 588, 612
BpuEI CTTGAG 1 cut(s) 215
BpuMI CCSGG 3 cut(s) 116, 135, 686
BsaBI GATNNNNATC 1 cut(s) 88
BsaJI CCNNGG 4 cut(s) 29, 390, 574, 685
Bsc4I CCNNNNNNNGG 1 cut(s) 134
Bse1I ACTGG 2 cut(s) 539, 709
Bse3DI GCAATG 3 cut(s) 356, 559, 765
Bse8I GATNNNNATC 1 cut(s) 88
BseBI CCWGG 1 cut(s) 30
BseDI CCNNGG 4 cut(s) 29, 390, 574, 685
BseGI GGATG 2 cut(s) 297, 468
BseJI GATNNNNATC 1 cut(s) 88
BseLI CCNNNNNNNGG 1 cut(s) 134
BseMI GCAATG 3 cut(s) 356, 559, 765
BseNI ACTGG 2 cut(s) 539, 709
BseXI GCAGC 1 cut(s) 373
Bsh1236I CGCG 1 cut(s) 74
BshFI GGCC 1 cut(s) 447
BsiHKAI GWGCWC 2 cut(s) 285, 373
BsiHKCI CYCGRG 2 cut(s) 286, 449
BsiSI CCGG 3 cut(s) 115, 134, 685
BslFI GGGAC 1 cut(s) 46
BslI CCNNNNNNNGG 1 cut(s) 134
BsmAI GTCTC 1 cut(s) 335
BsmFI GGGAC 1 cut(s) 46
BsmI GAATGC 3 cut(s) 395, 599, 784
BsnI GGCC 1 cut(s) 447
BsoBI CYCGRG 2 cut(s) 286, 449
Bsp1286I GDGCHC 3 cut(s) 285, 373, 492
Bsp143I GATC 5 cut(s) 150, 196, 654, 790, 829
BspACI CCGC 1 cut(s) 404
BspANI GGCC 1 cut(s) 447
BspFNI CGCG 1 cut(s) 74
BspLI GGNNCC 1 cut(s) 113
BspMAI CTGCAG 1 cut(s) 673
BspPI GGATC 1 cut(s) 158
BsrDI GCAATG 3 cut(s) 356, 559, 765
BsrI ACTGG 2 cut(s) 539, 709
BssECI CCNNGG 4 cut(s) 29, 390, 574, 685
BssMI GATC 5 cut(s) 150, 196, 654, 790, 829
BssT1I CCWWGG 1 cut(s) 574
Bst2UI CCWGG 1 cut(s) 30
Bst4CI ACNGT 1 cut(s) 508
BstAPI GCANNNNNTGC 1 cut(s) 401
BstC8I GCNNGC 1 cut(s) 807
BstDSI CCRYGG 1 cut(s) 390
BstF5I GGATG 2 cut(s) 297, 468
BstFNI CGCG 1 cut(s) 74
BstH2I RGCGCY 1 cut(s) 521
BstHHI GCGC 2 cut(s) 74, 520
BstKTI GATC 5 cut(s) 153, 199, 657, 793, 832
BstMAI GTCTC 1 cut(s) 335
BstMBI GATC 5 cut(s) 150, 196, 654, 790, 829
BstMWI GCNNNNNNNGC 3 cut(s) 185, 392, 401
BstNI CCWGG 1 cut(s) 30
BstNSI RCATGY 1 cut(s) 809
BstSCI CCNGG 4 cut(s) 28, 114, 133, 684
BstSFI CTRYAG 1 cut(s) 669
BstUI CGCG 1 cut(s) 74
BstV1I GCAGC 1 cut(s) 373
BstX2I RGATCY 1 cut(s) 790
BstYI RGATCY 1 cut(s) 790
BsuI GTATCC 1 cut(s) 100
BsuRI GGCC 1 cut(s) 447
BtgI CCRYGG 1 cut(s) 390
BtsCI GGATG 2 cut(s) 297, 468
Cac8I GCNNGC 1 cut(s) 807
CaiI CAGNNNCTG 1 cut(s) 815
CfoI GCGC 2 cut(s) 74, 520
Cfr13I GGNCC 3 cut(s) 112, 614, 682
Csp6I GTAC 3 cut(s) 157, 734, 812
CviAII CATG 4 cut(s) 20, 268, 660, 806
CviJI RGCY 7 cut(s) 166, 179, 211, 371, 447, 490, 534
CviKI_1 RGCY 7 cut(s) 166, 179, 211, 371, 447, 490, 534
CviQI GTAC 3 cut(s) 157, 734, 812
DpnI GATC 5 cut(s) 152, 198, 656, 792, 831
DpnII GATC 5 cut(s) 150, 196, 654, 790, 829
DrdI GACNNNNNNGTC 1 cut(s) 680
DriI GACNNNNNGTC 1 cut(s) 100
DseDI GACNNNNNNGTC 1 cut(s) 680
Eam1105I GACNNNNNGTC 1 cut(s) 100
Ecl136II GAGCTC 1 cut(s) 371
Eco130I CCWWGG 1 cut(s) 574
Eco147I AGGCCT 1 cut(s) 447
Eco24I GRGCYC 2 cut(s) 373, 492
Eco47I GGWCC 3 cut(s) 112, 614, 682
Eco47III AGCGCT 1 cut(s) 519
Eco53kI GAGCTC 1 cut(s) 371
Eco57I CTGAAG 1 cut(s) 384
Eco88I CYCGRG 2 cut(s) 286, 449
EcoICRI GAGCTC 1 cut(s) 371
EcoRII CCWGG 1 cut(s) 28
EcoT14I CCWWGG 1 cut(s) 574
EcoT38I GRGCYC 2 cut(s) 373, 492
ErhI CCWWGG 1 cut(s) 574
FaeI CATG 4 cut(s) 23, 271, 663, 809
FalI AAGNNNNNCTT 2 cut(s) 357, 389
FaqI GGGAC 1 cut(s) 46
FatI CATG 4 cut(s) 19, 267, 659, 805
FauNDI CATATG 1 cut(s) 757
Fnu4HI GCNGC 1 cut(s) 387
FokI GGATG 2 cut(s) 304, 455
FriOI GRGCYC 2 cut(s) 373, 492
Fsp4HI GCNGC 1 cut(s) 387
GlaI GCGC 2 cut(s) 73, 519
GluI GCNGC 1 cut(s) 387
HaeII RGCGCY 1 cut(s) 521
HaeIII GGCC 1 cut(s) 447
HapII CCGG 3 cut(s) 115, 134, 685
HhaI GCGC 2 cut(s) 74, 520
Hin1II CATG 4 cut(s) 23, 271, 663, 809
Hin6I GCGC 2 cut(s) 72, 518
HinP1I GCGC 2 cut(s) 72, 518
HincII GTYRAC 1 cut(s) 826
HindII GTYRAC 1 cut(s) 826
HinfI GANTC 1 cut(s) 124
HpaII CCGG 3 cut(s) 115, 134, 685
HphI GGTGA 1 cut(s) 595
Hpy166II GTNNAC 2 cut(s) 734, 826
Hpy188I TCNGA 6 cut(s) 83, 94, 381, 594, 618, 790
Hpy188III TCNNGA 1 cut(s) 774
Hpy8I GTNNAC 2 cut(s) 734, 826
HpyAV CCTTC 4 cut(s) 359, 488, 637, 641
HpyCH4III ACNGT 1 cut(s) 508
HpyCH4IV ACGT 1 cut(s) 35
HpyCH4V TGCA 7 cut(s) 552, 599, 671, 701, 743, 805, 809
HpyF10VI GCNNNNNNNGC 3 cut(s) 185, 392, 401
HpySE526I ACGT 1 cut(s) 35
Hsp92II CATG 4 cut(s) 23, 271, 663, 809
HspAI GCGC 2 cut(s) 72, 518
Kzo9I GATC 5 cut(s) 150, 196, 654, 790, 829
LmnI GCTCC 6 cut(s) 69, 163, 368, 515, 525, 752
Lsp1109I GCAGC 1 cut(s) 373
LweI GCATC 3 cut(s) 282, 588, 612
MaeII ACGT 1 cut(s) 35
MaeIII GTNAC 2 cut(s) 508, 693
MalI GATC 5 cut(s) 152, 198, 656, 792, 831
MboI GATC 5 cut(s) 150, 196, 654, 790, 829
MboII GAAGA 3 cut(s) 304, 344, 706
MflI RGATCY 1 cut(s) 790
MhlI GDGCHC 3 cut(s) 285, 373, 492
MluCI AATT 3 cut(s) 321, 483, 818
MmeI TCCRAC 1 cut(s) 117
MnlI CCTC 7 cut(s) 141, 234, 445, 458, 617, 673, 831
MseI TTAA 2 cut(s) 243, 566
MslI CAYNNNNRTG 1 cut(s) 270
MspI CCGG 3 cut(s) 115, 134, 685
MspR9I CCNGG 4 cut(s) 30, 116, 135, 686
Mva1269I GAATGC 3 cut(s) 395, 599, 784
MvaI CCWGG 1 cut(s) 30
MvnI CGCG 1 cut(s) 74
MwoI GCNNNNNNNGC 3 cut(s) 185, 392, 401
NciI CCSGG 3 cut(s) 116, 135, 686
NdeI CATATG 1 cut(s) 757
NdeII GATC 5 cut(s) 150, 196, 654, 790, 829
NlaIII CATG 4 cut(s) 23, 271, 663, 809
NlaIV GGNNCC 1 cut(s) 113
NspI RCATGY 1 cut(s) 809
PaeI GCATGC 1 cut(s) 809
PaeR7I CTCGAG 1 cut(s) 449
PceI AGGCCT 1 cut(s) 447
PctI GAATGC 3 cut(s) 395, 599, 784
PfeI GAWTC 1 cut(s) 124
PkrI GCNGC 1 cut(s) 388
Psp124BI GAGCTC 1 cut(s) 373
Psp6I CCWGG 1 cut(s) 28
PspGI CCWGG 1 cut(s) 28
PspN4I GGNNCC 1 cut(s) 113
PspPI GGNCC 3 cut(s) 112, 614, 682
PspXI VCTCGAGB 1 cut(s) 449
PsrI GAACNNNNNNTAC 2 cut(s) 401, 433
PstI CTGCAG 1 cut(s) 673
PstNI CAGNNNCTG 1 cut(s) 815
PsuI RGATCY 1 cut(s) 790
RsaI GTAC 3 cut(s) 158, 735, 813
RsaNI GTAC 3 cut(s) 157, 734, 812
RseI CAYNNNNRTG 1 cut(s) 270
SacI GAGCTC 1 cut(s) 373
SaqAI TTAA 2 cut(s) 243, 566
SatI GCNGC 1 cut(s) 387
Sau3AI GATC 5 cut(s) 150, 196, 654, 790, 829
Sau96I GGNCC 3 cut(s) 112, 614, 682
ScaI AGTACT 1 cut(s) 158
ScrFI CCNGG 4 cut(s) 30, 116, 135, 686
SduI GDGCHC 3 cut(s) 285, 373, 492
SetI ASST 7 cut(s) 38, 168, 213, 373, 536, 684, 817
SfaNI GCATC 3 cut(s) 282, 588, 612
SfcI CTRYAG 1 cut(s) 669
Sfr274I CTCGAG 1 cut(s) 449
SinI GGWCC 3 cut(s) 112, 614, 682
SlaI CTCGAG 1 cut(s) 449
SmiMI CAYNNNNRTG 1 cut(s) 270
SmlI CTYRAG 2 cut(s) 230, 449
SmoI CTYRAG 2 cut(s) 230, 449
SphI GCATGC 1 cut(s) 809
Sse9I AATT 3 cut(s) 321, 483, 818
SseBI AGGCCT 1 cut(s) 447
SsiI CCGC 1 cut(s) 404
SstI GAGCTC 1 cut(s) 373
StuI AGGCCT 1 cut(s) 447
StyD4I CCNGG 4 cut(s) 28, 114, 133, 684
StyI CCWWGG 1 cut(s) 574
TaaI ACNGT 1 cut(s) 508
TaiI ACGT 1 cut(s) 38
TaqI TCGA 2 cut(s) 207, 450
TasI AATT 3 cut(s) 321, 483, 818
TatI WGTACW 2 cut(s) 156, 733
TfiI GAWTC 1 cut(s) 124
Tru1I TTAA 2 cut(s) 243, 566
Tru9I TTAA 2 cut(s) 243, 566
TseI GCWGC 1 cut(s) 386
TspDTI ATGAA 2 cut(s) 284, 754
VpaK11BI GGWCC 3 cut(s) 112, 614, 682
XceI RCATGY 1 cut(s) 809
XhoI CTCGAG 1 cut(s) 449
ZrmI AGTACT 1 cut(s) 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.