MD06G1014200.v1.1

Transmembrane protein 45B-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0008026
Physical Location & Seq
Forward (+)
1803847 .. 1807377
3531 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1014200.v1.1.491

Sequence Viewer

Length: 816 bp
ATGGGTTCTTTCAAAGGTCATATTCTGCCAGGGACTTTGTTTTTGGTTGTTGGGGTGTGGCACGTATGGTGCTCGCTGGTGAGATATGTGTCAAACCCCAAGTCCTTTCGGGTACATGTATGGAACCCAGTTCCCGGTGTTGATGGGAGGCTTAAGTATTTTGAGCTTTATCTTATAGCAGTTGGTGCTTTCATTGATATGTGTATCGAGCTTTTGTATTCGACCCATCTCAAGTTCTTTGTAGATGGAGTCTTGAACCCTCATCACATGAATGATTTCGAGCACTCGGGGATGCTTCTTATGTTCTTTATCTTTGGGGTTGTCACCCTGATCTCACAGGAGACAAGATTTCTTCCGATGCCTGAAGGAGCTCTTGCTCTGATTGCTGCTACAGCATTCTGTGCAGAGTATCTCCTTTTCTACTTTCACTCAACAACTCATAAAGGCCTCGAGGGGTATTATCACATCCTCCTTGTCCTCCTAATCGGGCTCTGCATAGTATCAACTGTAGCTGGAGCCCTCTTACCAACCAGCTTTCCAATTGATTTATGTAGCGGTATTGCCATAACTCTCCAAGGCCTCTGGTTTTATCAGACTGCGTTCACTCTCTACGGCCCCATGATGCCAGATGGTTGTCAGCTCAAGGATGATATGATCTCATGTCATTCTGTCGACAATGAGGTCCGAGGTGAGTTGCTTGCAAACTTCCAGTTCTTTACCATGGTTCTTAGTGTACTTGTTGCAGTTGTGGGAGGATATGGTTTTGTTGCCTCAAGATTTGGGCATTCTGATCAACGGAGCTTGCGTGCAGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF716

Protein Analysis

272

Amino Acids

30.22

Weight (kDa)

6.14

Isoelectric Point (pI)

27.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF716 PF04819 123 - 253 2.7e-45 Family of unknown function (DUF716)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 680
AbsI CCTCGAGG 1 cut(s) 449
AccI GTMKAC 1 cut(s) 672
AciI CCGC 1 cut(s) 555
AcuI CTGAAG 1 cut(s) 384
AfaI GTAC 2 cut(s) 114, 735
AfiI CCNNNNNNNGG 1 cut(s) 134
AflII CTTAAG 1 cut(s) 152
AflIII ACRYGT 1 cut(s) 115
AgsI TTSAA 2 cut(s) 13, 256
AjnI CCWGG 1 cut(s) 28
AluBI AGCT 7 cut(s) 166, 211, 371, 512, 534, 640, 801
AluI AGCT 7 cut(s) 166, 211, 371, 512, 534, 640, 801
Alw21I GWGCWC 3 cut(s) 74, 285, 373
Alw26I GTCTC 1 cut(s) 335
Ama87I CYCGRG 2 cut(s) 286, 449
AoxI GGCC 3 cut(s) 445, 577, 613
ApeKI GCWGC 1 cut(s) 386
ArsI GACNNNNNNTTYG 4 cut(s) 25, 57, 86, 118
Asp700I GAANNNNTTC 1 cut(s) 275
AspS9I GGNCC 2 cut(s) 614, 682
AsuC2I CCSGG 1 cut(s) 135
AsuHPI GGTGA 3 cut(s) 91, 316, 701
AvaI CYCGRG 2 cut(s) 286, 449
AvaII GGWCC 1 cut(s) 682
BanII GRGCYC 3 cut(s) 373, 492, 520
Bbv12I GWGCWC 3 cut(s) 74, 285, 373
BbvI GCAGC 1 cut(s) 373
BccI CCATC 4 cut(s) 137, 234, 239, 623
BceAI ACGGC 1 cut(s) 628
BciT130I CCWGG 1 cut(s) 30
BclI TGATCA 1 cut(s) 790
BcnI CCSGG 1 cut(s) 135
BcoDI GTCTC 1 cut(s) 335
BfmI CTRYAG 2 cut(s) 390, 507
BfrI CTTAAG 1 cut(s) 152
BisI GCNGC 1 cut(s) 387
BlsI GCNGC 1 cut(s) 388
Bme1390I CCNGG 2 cut(s) 30, 135
Bme18I GGWCC 1 cut(s) 682
BmeT110I CYCGRG 2 cut(s) 286, 449
BmgT120I GGNCC 2 cut(s) 614, 682
BmiI GGNNCC 3 cut(s) 125, 517, 616
BmrFI CCNGG 2 cut(s) 30, 135
BmrI ACTGGG 1 cut(s) 122
BmsI GCATC 3 cut(s) 282, 348, 612
BmuI ACTGGG 1 cut(s) 122
BpmI CTGGAG 1 cut(s) 534
BpuEI CTTGAG 3 cut(s) 215, 626, 757
BpuMI CCSGG 1 cut(s) 135
BsaAI YACGTR 1 cut(s) 64
BsaJI CCNNGG 4 cut(s) 29, 574, 685, 720
BsaXI ACNNNNNCTCC 1 cut(s) 790
Bsc4I CCNNNNNNNGG 1 cut(s) 134
Bse1I ACTGG 2 cut(s) 128, 709
BseBI CCWGG 1 cut(s) 30
BseDI CCNNGG 4 cut(s) 29, 574, 685, 720
BseGI GGATG 3 cut(s) 297, 465, 652
BseLI CCNNNNNNNGG 1 cut(s) 134
BseNI ACTGG 2 cut(s) 128, 709
BseXI GCAGC 1 cut(s) 373
BsgI GTGCAG 1 cut(s) 423
BshFI GGCC 3 cut(s) 447, 579, 615
BsiHKAI GWGCWC 3 cut(s) 74, 285, 373
BsiHKCI CYCGRG 2 cut(s) 286, 449
BsiSI CCGG 1 cut(s) 135
BslFI GGGAC 1 cut(s) 46
BslI CCNNNNNNNGG 1 cut(s) 134
BsmAI GTCTC 1 cut(s) 335
BsmFI GGGAC 1 cut(s) 46
BsmI GAATGC 2 cut(s) 395, 784
BsnI GGCC 3 cut(s) 447, 579, 615
BsoBI CYCGRG 2 cut(s) 286, 449
Bsp1286I GDGCHC 5 cut(s) 74, 285, 373, 492, 520
Bsp143I GATC 3 cut(s) 330, 654, 790
Bsp19I CCATGG 1 cut(s) 720
BspACI CCGC 1 cut(s) 555
BspANI GGCC 3 cut(s) 447, 579, 615
BspLI GGNNCC 3 cut(s) 125, 517, 616
BspTI CTTAAG 1 cut(s) 152
BsrI ACTGG 2 cut(s) 128, 709
BssECI CCNNGG 4 cut(s) 29, 574, 685, 720
BssMI GATC 3 cut(s) 330, 654, 790
BssT1I CCWWGG 2 cut(s) 574, 720
Bst2UI CCWGG 1 cut(s) 30
Bst4CI ACNGT 1 cut(s) 508
BstAFI CTTAAG 1 cut(s) 152
BstAPI GCANNNNNTGC 2 cut(s) 185, 401
BstBAI YACGTR 1 cut(s) 64
BstC8I GCNNGC 4 cut(s) 74, 699, 803, 807
BstDEI CTNAG 1 cut(s) 728
BstDSI CCRYGG 1 cut(s) 720
BstF5I GGATG 3 cut(s) 297, 465, 652
BstKTI GATC 3 cut(s) 333, 657, 793
BstMAI GTCTC 1 cut(s) 335
BstMBI GATC 3 cut(s) 330, 654, 790
BstMWI GCNNNNNNNGC 4 cut(s) 185, 383, 392, 401
BstNI CCWGG 1 cut(s) 30
BstNSI RCATGY 1 cut(s) 119
BstSCI CCNGG 2 cut(s) 28, 133
BstSFI CTRYAG 2 cut(s) 390, 507
BstV1I GCAGC 1 cut(s) 373
BsuRI GGCC 3 cut(s) 447, 579, 615
BtgI CCRYGG 1 cut(s) 720
BtsCI GGATG 3 cut(s) 297, 465, 652
Cac8I GCNNGC 4 cut(s) 74, 699, 803, 807
Cfr13I GGNCC 2 cut(s) 614, 682
Csp6I GTAC 2 cut(s) 113, 734
CviAII CATG 5 cut(s) 116, 268, 619, 660, 721
CviQI GTAC 2 cut(s) 113, 734
DdeI CTNAG 1 cut(s) 728
DpnI GATC 3 cut(s) 332, 656, 792
DpnII GATC 3 cut(s) 330, 654, 790
DrdI GACNNNNNNGTC 1 cut(s) 680
DseDI GACNNNNNNGTC 1 cut(s) 680
Ecl136II GAGCTC 1 cut(s) 371
Eco130I CCWWGG 2 cut(s) 574, 720
Eco147I AGGCCT 2 cut(s) 447, 579
Eco24I GRGCYC 3 cut(s) 373, 492, 520
Eco47I GGWCC 1 cut(s) 682
Eco53kI GAGCTC 1 cut(s) 371
Eco57I CTGAAG 1 cut(s) 384
Eco88I CYCGRG 2 cut(s) 286, 449
EcoICRI GAGCTC 1 cut(s) 371
EcoRII CCWGG 1 cut(s) 28
EcoT14I CCWWGG 2 cut(s) 574, 720
EcoT38I GRGCYC 3 cut(s) 373, 492, 520
ErhI CCWWGG 2 cut(s) 574, 720
FaeI CATG 5 cut(s) 119, 271, 622, 663, 724
FalI AAGNNNNNCTT 2 cut(s) 357, 389
FaqI GGGAC 1 cut(s) 46
FatI CATG 5 cut(s) 115, 267, 618, 659, 720
FbaI TGATCA 1 cut(s) 790
FblI GTMKAC 1 cut(s) 672
Fnu4HI GCNGC 1 cut(s) 387
FokI GGATG 3 cut(s) 304, 452, 659
FriOI GRGCYC 3 cut(s) 373, 492, 520
Fsp4HI GCNGC 1 cut(s) 387
GluI GCNGC 1 cut(s) 387
GsuI CTGGAG 1 cut(s) 534
HaeIII GGCC 3 cut(s) 447, 579, 615
HapII CCGG 1 cut(s) 135
Hin1II CATG 5 cut(s) 119, 271, 622, 663, 724
HincII GTYRAC 1 cut(s) 673
HindII GTYRAC 1 cut(s) 673
HinfI GANTC 1 cut(s) 249
HpaII CCGG 1 cut(s) 135
HphI GGTGA 3 cut(s) 91, 316, 701
Hpy166II GTNNAC 3 cut(s) 603, 673, 734
Hpy188I TCNGA 5 cut(s) 357, 381, 594, 686, 790
Hpy188III TCNNGA 2 cut(s) 253, 774
Hpy8I GTNNAC 3 cut(s) 603, 673, 734
HpyAV CCTTC 1 cut(s) 359
HpyCH4III ACNGT 1 cut(s) 508
HpyCH4IV ACGT 1 cut(s) 63
HpyCH4V TGCA 5 cut(s) 404, 495, 701, 743, 809
HpyF10VI GCNNNNNNNGC 4 cut(s) 185, 383, 392, 401
HpyF3I CTNAG 1 cut(s) 728
HpySE526I ACGT 1 cut(s) 63
Hsp92II CATG 5 cut(s) 119, 271, 622, 663, 724
Ksp22I TGATCA 1 cut(s) 790
Kzo9I GATC 3 cut(s) 330, 654, 790
LmnI GCTCC 3 cut(s) 368, 515, 798
Lsp1109I GCAGC 1 cut(s) 373
LweI GCATC 3 cut(s) 282, 348, 612
MaeII ACGT 1 cut(s) 63
MaeIII GTNAC 1 cut(s) 322
MalI GATC 3 cut(s) 332, 656, 792
MboI GATC 3 cut(s) 330, 654, 790
MboII GAAGA 1 cut(s) 344
MfeI CAATTG 1 cut(s) 540
MhlI GDGCHC 5 cut(s) 74, 285, 373, 492, 520
MluCI AATT 1 cut(s) 540
MlyI GAGTC 1 cut(s) 258
MroXI GAANNNNTTC 1 cut(s) 275
MseI TTAA 1 cut(s) 153
MslI CAYNNNNRTG 2 cut(s) 197, 270
MspCI CTTAAG 1 cut(s) 152
MspI CCGG 1 cut(s) 135
MspR9I CCNGG 2 cut(s) 30, 135
MunI CAATTG 1 cut(s) 540
Mva1269I GAATGC 2 cut(s) 395, 784
MvaI CCWGG 1 cut(s) 30
MwoI GCNNNNNNNGC 4 cut(s) 185, 383, 392, 401
NciI CCSGG 1 cut(s) 135
NcoI CCATGG 1 cut(s) 720
NdeII GATC 3 cut(s) 330, 654, 790
NlaIII CATG 5 cut(s) 119, 271, 622, 663, 724
NlaIV GGNNCC 3 cut(s) 125, 517, 616
NmuCI GTSAC 1 cut(s) 322
NspI RCATGY 1 cut(s) 119
PaeR7I CTCGAG 1 cut(s) 449
PceI AGGCCT 2 cut(s) 447, 579
PciI ACATGT 1 cut(s) 115
PcsI WCGNNNNNNNCGW 1 cut(s) 802
PctI GAATGC 2 cut(s) 395, 784
PdmI GAANNNNTTC 1 cut(s) 275
PkrI GCNGC 1 cut(s) 388
PleI GAGTC 1 cut(s) 257
PpsI GAGTC 1 cut(s) 257
Ppu21I YACGTR 1 cut(s) 64
PscI ACATGT 1 cut(s) 115
Psp124BI GAGCTC 1 cut(s) 373
Psp6I CCWGG 1 cut(s) 28
PspGI CCWGG 1 cut(s) 28
PspN4I GGNNCC 3 cut(s) 125, 517, 616
PspPI GGNCC 2 cut(s) 614, 682
PspXI VCTCGAGB 1 cut(s) 449
RsaI GTAC 2 cut(s) 114, 735
RsaNI GTAC 2 cut(s) 113, 734
RseI CAYNNNNRTG 2 cut(s) 197, 270
SacI GAGCTC 1 cut(s) 373
SalI GTCGAC 1 cut(s) 671
SaqAI TTAA 1 cut(s) 153
SatI GCNGC 1 cut(s) 387
Sau3AI GATC 3 cut(s) 330, 654, 790
Sau96I GGNCC 2 cut(s) 614, 682
SchI GAGTC 1 cut(s) 258
ScrFI CCNGG 2 cut(s) 30, 135
SduI GDGCHC 5 cut(s) 74, 285, 373, 492, 520
SfaNI GCATC 3 cut(s) 282, 348, 612
SfcI CTRYAG 2 cut(s) 390, 507
Sfr274I CTCGAG 1 cut(s) 449
SinI GGWCC 1 cut(s) 682
SlaI CTCGAG 1 cut(s) 449
SmiMI CAYNNNNRTG 2 cut(s) 197, 270
SmlI CTYRAG 5 cut(s) 152, 230, 449, 641, 772
SmoI CTYRAG 5 cut(s) 152, 230, 449, 641, 772
Sse9I AATT 1 cut(s) 540
SseBI AGGCCT 2 cut(s) 447, 579
SsiI CCGC 1 cut(s) 555
SstI GAGCTC 1 cut(s) 373
StuI AGGCCT 2 cut(s) 447, 579
StyD4I CCNGG 2 cut(s) 28, 133
StyI CCWWGG 2 cut(s) 574, 720
TaaI ACNGT 1 cut(s) 508
TaiI ACGT 1 cut(s) 66
TaqI TCGA 5 cut(s) 207, 221, 279, 450, 672
TasI AATT 1 cut(s) 540
TatI WGTACW 1 cut(s) 733
Tru1I TTAA 1 cut(s) 153
Tru9I TTAA 1 cut(s) 153
TseFI GTSAC 1 cut(s) 322
TseI GCWGC 1 cut(s) 386
Tsp45I GTSAC 1 cut(s) 322
TspDTI ATGAA 2 cut(s) 181, 284
TspGWI ACGGA 1 cut(s) 811
Vha464I CTTAAG 1 cut(s) 152
VpaK11BI GGWCC 1 cut(s) 682
XceI RCATGY 1 cut(s) 119
XhoI CTCGAG 1 cut(s) 449
XmiI GTMKAC 1 cut(s) 672
XmnI GAANNNNTTC 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.