MD00G1000700.v1.1

Transmembrane protein 45B-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0008026
Physical Location & Seq
Forward (+)
105542 .. 109696
4155 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1000700.v1.1.491

Sequence Viewer

Length: 813 bp
ATGGGTTCTTTCCCAGGTCATGTTCTGCCAGGGACATTGTTTTTGGTTATTGGGGTGTGGCACGTATGGCGCTCGCTGGTGAGATATGTATCCAACCCCAAGTCCTTTAGGGTACATGTATGGAACCCGGTTCCCGGTGTTGATGGGAGGCTTAAGTATTTTGAGCTTTATCTTATAGCAGTTGGTGCTTTCATTGATATGTGTATTGAGCTTTTGTATTCGACCCATCTCAAGTTCTTTGTTAATGGAGTCTTGAACCCTCATCACATGAATGATTTCGAGCACTCGGGGATGCTTCTAATGTTCTTTATCTTTGGGGTTATCACCCTGATCTCACAGGAGACAAGATTTCTTCCGATGCCCGAAGGCGCTCTTTGTCTGATTGCTGCTACAGCATTCTGTGCAGAGTACCTTCTTTTCTACTTTCACTCAACAACTCATAAAGGCCTTGAGGGGTATTACCACATCCTCCTTGTCCTCCTAATCGGGCTTTGCATAGTATCCTCTGTTGCCGGAGCCCTCTTACCAACCAGCTTTCCAATTGATTTATGTAGCGGTATTGGCATAACTCTCCAAGGCCTCTGGTTTTATCAGACTGCGTTCACTCTCTATGGCCCCATGATGCCAGATGGTTGTGGGCTCAAGGATGATATGGTCTCATGTCATTCTGTCGACAGTGAGGTCCGGGGTGAGTTGCTTGCAAACTTCCAGTTCTTTATCATGGTTCTTTGCGTACTCGTTGCAGTTGTGGGAGGATATGGTTTTGTTGCCTCAAGATTTGGGCATTCTGATGAACGGAGCTTGCGTGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF716

Protein Analysis

271

Amino Acids

30.11

Weight (kDa)

6.15

Isoelectric Point (pI)

31.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF716 PF04819 123 - 253 2.6e-45 Family of unknown function (DUF716)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 680
AccI GTMKAC 1 cut(s) 672
AciI CCGC 1 cut(s) 555
AfaI GTAC 3 cut(s) 114, 410, 735
AfiI CCNNNNNNNGG 1 cut(s) 134
AflII CTTAAG 1 cut(s) 152
AflIII ACRYGT 1 cut(s) 115
AgsI TTSAA 1 cut(s) 256
AjnI CCWGG 2 cut(s) 13, 28
AluBI AGCT 4 cut(s) 166, 211, 534, 801
AluI AGCT 4 cut(s) 166, 211, 534, 801
Alw21I GWGCWC 1 cut(s) 285
Alw26I GTCTC 2 cut(s) 335, 661
Ama87I CYCGRG 1 cut(s) 286
AoxI GGCC 3 cut(s) 445, 577, 613
ApeKI GCWGC 1 cut(s) 386
ArsI GACNNNNNNTTYG 2 cut(s) 25, 57
Asp700I GAANNNNTTC 1 cut(s) 275
AspLEI GCGC 2 cut(s) 72, 371
AspS9I GGNCC 2 cut(s) 614, 682
AsuC2I CCSGG 3 cut(s) 128, 135, 686
AsuHPI GGTGA 3 cut(s) 91, 316, 701
AvaI CYCGRG 1 cut(s) 286
AvaII GGWCC 1 cut(s) 682
BanII GRGCYC 2 cut(s) 520, 642
Bbv12I GWGCWC 1 cut(s) 285
BbvI GCAGC 1 cut(s) 373
BccI CCATC 3 cut(s) 137, 234, 623
BciT130I CCWGG 2 cut(s) 15, 30
BciVI GTATCC 2 cut(s) 100, 511
BcnI CCSGG 3 cut(s) 128, 135, 686
BcoDI GTCTC 2 cut(s) 335, 661
BfmI CTRYAG 1 cut(s) 390
BfoI RGCGCY 2 cut(s) 73, 372
BfrI CTTAAG 1 cut(s) 152
BfuI GTATCC 2 cut(s) 100, 511
BisI GCNGC 1 cut(s) 387
BlsI GCNGC 1 cut(s) 388
Bme1390I CCNGG 5 cut(s) 15, 30, 128, 135, 686
Bme18I GGWCC 1 cut(s) 682
BmeT110I CYCGRG 1 cut(s) 286
BmgT120I GGNCC 2 cut(s) 614, 682
BmiI GGNNCC 4 cut(s) 125, 132, 517, 616
BmrFI CCNGG 5 cut(s) 15, 30, 128, 135, 686
BmsI GCATC 3 cut(s) 282, 348, 612
BpuEI CTTGAG 4 cut(s) 215, 470, 626, 757
BpuMI CCSGG 3 cut(s) 128, 135, 686
BsaAI YACGTR 1 cut(s) 64
BsaBI GATNNNNATC 1 cut(s) 88
BsaI GGTCTC 1 cut(s) 661
BsaJI CCNNGG 4 cut(s) 13, 29, 574, 685
BsaXI ACNNNNNCTCC 1 cut(s) 790
Bsc4I CCNNNNNNNGG 1 cut(s) 134
Bse1I ACTGG 1 cut(s) 709
Bse8I GATNNNNATC 1 cut(s) 88
BseBI CCWGG 2 cut(s) 15, 30
BseDI CCNNGG 4 cut(s) 13, 29, 574, 685
BseGI GGATG 3 cut(s) 297, 465, 652
BseJI GATNNNNATC 1 cut(s) 88
BseLI CCNNNNNNNGG 1 cut(s) 134
BseNI ACTGG 1 cut(s) 709
BseXI GCAGC 1 cut(s) 373
BsgI GTGCAG 1 cut(s) 423
BshFI GGCC 3 cut(s) 447, 579, 615
BsiHKAI GWGCWC 1 cut(s) 285
BsiHKCI CYCGRG 1 cut(s) 286
BsiSI CCGG 4 cut(s) 128, 135, 513, 685
BslFI GGGAC 1 cut(s) 46
BslI CCNNNNNNNGG 1 cut(s) 134
BsmAI GTCTC 2 cut(s) 335, 661
BsmFI GGGAC 1 cut(s) 46
BsmI GAATGC 2 cut(s) 395, 784
BsnI GGCC 3 cut(s) 447, 579, 615
Bso31I GGTCTC 1 cut(s) 661
BsoBI CYCGRG 1 cut(s) 286
Bsp1286I GDGCHC 3 cut(s) 285, 520, 642
Bsp143I GATC 1 cut(s) 330
BspACI CCGC 1 cut(s) 555
BspANI GGCC 3 cut(s) 447, 579, 615
BspLI GGNNCC 4 cut(s) 125, 132, 517, 616
BspTI CTTAAG 1 cut(s) 152
BspTNI GGTCTC 1 cut(s) 661
BsrI ACTGG 1 cut(s) 709
BssECI CCNNGG 4 cut(s) 13, 29, 574, 685
BssMI GATC 1 cut(s) 330
BssT1I CCWWGG 1 cut(s) 574
Bst2UI CCWGG 2 cut(s) 15, 30
Bst4CI ACNGT 1 cut(s) 677
BstAFI CTTAAG 1 cut(s) 152
BstAPI GCANNNNNTGC 2 cut(s) 185, 401
BstBAI YACGTR 1 cut(s) 64
BstC8I GCNNGC 3 cut(s) 74, 699, 803
BstF5I GGATG 3 cut(s) 297, 465, 652
BstH2I RGCGCY 2 cut(s) 73, 372
BstHHI GCGC 2 cut(s) 72, 371
BstKTI GATC 1 cut(s) 333
BstMAI GTCTC 2 cut(s) 335, 661
BstMBI GATC 1 cut(s) 330
BstMWI GCNNNNNNNGC 5 cut(s) 67, 185, 392, 401, 561
BstNI CCWGG 2 cut(s) 15, 30
BstNSI RCATGY 1 cut(s) 119
BstSCI CCNGG 5 cut(s) 13, 28, 126, 133, 684
BstSFI CTRYAG 1 cut(s) 390
BstV1I GCAGC 1 cut(s) 373
BsuI GTATCC 2 cut(s) 100, 511
BsuRI GGCC 3 cut(s) 447, 579, 615
BtsCI GGATG 3 cut(s) 297, 465, 652
BtsIMutI CAGTG 1 cut(s) 682
Cac8I GCNNGC 3 cut(s) 74, 699, 803
CfoI GCGC 2 cut(s) 72, 371
Cfr13I GGNCC 2 cut(s) 614, 682
Csp6I GTAC 3 cut(s) 113, 409, 734
CviAII CATG 6 cut(s) 20, 116, 268, 619, 660, 721
CviQI GTAC 3 cut(s) 113, 409, 734
DpnI GATC 1 cut(s) 332
DpnII GATC 1 cut(s) 330
DrdI GACNNNNNNGTC 1 cut(s) 680
DseDI GACNNNNNNGTC 1 cut(s) 680
Eco130I CCWWGG 1 cut(s) 574
Eco147I AGGCCT 2 cut(s) 447, 579
Eco24I GRGCYC 2 cut(s) 520, 642
Eco31I GGTCTC 1 cut(s) 661
Eco47I GGWCC 1 cut(s) 682
Eco88I CYCGRG 1 cut(s) 286
EcoRII CCWGG 2 cut(s) 13, 28
EcoT14I CCWWGG 1 cut(s) 574
EcoT38I GRGCYC 2 cut(s) 520, 642
ErhI CCWWGG 1 cut(s) 574
FaeI CATG 6 cut(s) 23, 119, 271, 622, 663, 724
FalI AAGNNNNNCTT 2 cut(s) 357, 389
FaqI GGGAC 1 cut(s) 46
FatI CATG 6 cut(s) 19, 115, 267, 618, 659, 720
FblI GTMKAC 1 cut(s) 672
Fnu4HI GCNGC 1 cut(s) 387
FokI GGATG 3 cut(s) 304, 452, 659
FriOI GRGCYC 2 cut(s) 520, 642
Fsp4HI GCNGC 1 cut(s) 387
GlaI GCGC 2 cut(s) 71, 370
GluI GCNGC 1 cut(s) 387
HaeII RGCGCY 2 cut(s) 73, 372
HaeIII GGCC 3 cut(s) 447, 579, 615
HapII CCGG 4 cut(s) 128, 135, 513, 685
HhaI GCGC 2 cut(s) 72, 371
Hin1II CATG 6 cut(s) 23, 119, 271, 622, 663, 724
Hin6I GCGC 2 cut(s) 70, 369
HinP1I GCGC 2 cut(s) 70, 369
HincII GTYRAC 1 cut(s) 673
HindII GTYRAC 1 cut(s) 673
HinfI GANTC 1 cut(s) 249
HpaII CCGG 4 cut(s) 128, 135, 513, 685
HphI GGTGA 3 cut(s) 91, 316, 701
Hpy166II GTNNAC 2 cut(s) 603, 673
Hpy188I TCNGA 4 cut(s) 357, 381, 594, 790
Hpy188III TCNNGA 2 cut(s) 253, 774
Hpy8I GTNNAC 2 cut(s) 603, 673
HpyAV CCTTC 2 cut(s) 359, 422
HpyCH4III ACNGT 1 cut(s) 677
HpyCH4IV ACGT 1 cut(s) 63
HpyCH4V TGCA 4 cut(s) 404, 495, 701, 743
HpyF10VI GCNNNNNNNGC 5 cut(s) 67, 185, 392, 401, 561
HpySE526I ACGT 1 cut(s) 63
Hsp92II CATG 6 cut(s) 23, 119, 271, 622, 663, 724
HspAI GCGC 2 cut(s) 70, 369
Kzo9I GATC 1 cut(s) 330
LmnI GCTCC 2 cut(s) 515, 798
Lsp1109I GCAGC 1 cut(s) 373
LweI GCATC 3 cut(s) 282, 348, 612
MaeII ACGT 1 cut(s) 63
MalI GATC 1 cut(s) 332
MboI GATC 1 cut(s) 330
MboII GAAGA 1 cut(s) 344
MfeI CAATTG 1 cut(s) 540
MhlI GDGCHC 3 cut(s) 285, 520, 642
MluCI AATT 1 cut(s) 540
MlyI GAGTC 1 cut(s) 258
MmeI TCCRAC 1 cut(s) 117
MroXI GAANNNNTTC 1 cut(s) 275
MseI TTAA 2 cut(s) 153, 243
MslI CAYNNNNRTG 3 cut(s) 197, 270, 789
MspCI CTTAAG 1 cut(s) 152
MspI CCGG 4 cut(s) 128, 135, 513, 685
MspR9I CCNGG 5 cut(s) 15, 30, 128, 135, 686
MunI CAATTG 1 cut(s) 540
Mva1269I GAATGC 2 cut(s) 395, 784
MvaI CCWGG 2 cut(s) 15, 30
MwoI GCNNNNNNNGC 5 cut(s) 67, 185, 392, 401, 561
NciI CCSGG 3 cut(s) 128, 135, 686
NdeII GATC 1 cut(s) 330
NlaIII CATG 6 cut(s) 23, 119, 271, 622, 663, 724
NlaIV GGNNCC 4 cut(s) 125, 132, 517, 616
NspI RCATGY 1 cut(s) 119
PceI AGGCCT 2 cut(s) 447, 579
PciI ACATGT 1 cut(s) 115
PcsI WCGNNNNNNNCGW 1 cut(s) 802
PctI GAATGC 2 cut(s) 395, 784
PdmI GAANNNNTTC 1 cut(s) 275
PkrI GCNGC 1 cut(s) 388
PleI GAGTC 1 cut(s) 257
PpsI GAGTC 1 cut(s) 257
Ppu21I YACGTR 1 cut(s) 64
PscI ACATGT 1 cut(s) 115
Psp6I CCWGG 2 cut(s) 13, 28
PspGI CCWGG 2 cut(s) 13, 28
PspN4I GGNNCC 4 cut(s) 125, 132, 517, 616
PspPI GGNCC 2 cut(s) 614, 682
RsaI GTAC 3 cut(s) 114, 410, 735
RsaNI GTAC 3 cut(s) 113, 409, 734
RseI CAYNNNNRTG 3 cut(s) 197, 270, 789
SalI GTCGAC 1 cut(s) 671
SaqAI TTAA 2 cut(s) 153, 243
SatI GCNGC 1 cut(s) 387
Sau3AI GATC 1 cut(s) 330
Sau96I GGNCC 2 cut(s) 614, 682
SchI GAGTC 1 cut(s) 258
ScrFI CCNGG 5 cut(s) 15, 30, 128, 135, 686
SduI GDGCHC 3 cut(s) 285, 520, 642
SetI ASST 8 cut(s) 19, 66, 168, 213, 414, 536, 684, 803
SfaNI GCATC 3 cut(s) 282, 348, 612
SfcI CTRYAG 1 cut(s) 390
SinI GGWCC 1 cut(s) 682
SmiMI CAYNNNNRTG 3 cut(s) 197, 270, 789
SmlI CTYRAG 5 cut(s) 152, 230, 449, 641, 772
SmoI CTYRAG 5 cut(s) 152, 230, 449, 641, 772
Sse9I AATT 1 cut(s) 540
SseBI AGGCCT 2 cut(s) 447, 579
SsiI CCGC 1 cut(s) 555
StuI AGGCCT 2 cut(s) 447, 579
StyD4I CCNGG 5 cut(s) 13, 28, 126, 133, 684
StyI CCWWGG 1 cut(s) 574
TaaI ACNGT 1 cut(s) 677
TaiI ACGT 1 cut(s) 66
TaqI TCGA 3 cut(s) 221, 279, 672
TasI AATT 1 cut(s) 540
Tru1I TTAA 2 cut(s) 153, 243
Tru9I TTAA 2 cut(s) 153, 243
TscAI CASTG 1 cut(s) 682
TseI GCWGC 1 cut(s) 386
TspDTI ATGAA 3 cut(s) 181, 284, 807
TspGWI ACGGA 1 cut(s) 811
TspRI CASTG 1 cut(s) 682
Vha464I CTTAAG 1 cut(s) 152
VpaK11BI GGWCC 1 cut(s) 682
XceI RCATGY 1 cut(s) 119
XmiI GTMKAC 1 cut(s) 672
XmnI GAANNNNTTC 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.