FvH4_5g25960

Protein CUP-SHAPED COTYLEDON

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0013567
Physical Location & Seq
Reverse (-)
17465354 .. 17468430
3077 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g25960.t3

Sequence Viewer

Length: 1233 bp
ATGTTGGGTTTGATGGAAGAAATGAGTGAGCTGCTGGGGAGGGAAGAGGTGAACGAACAGGGTCTTCCACCTGGTTTCAGGTTCCACCCAACTGATGAGGAGCTCATCACCTTTTACCTCGCTTCCAAGGTCTACAATGGCGCTTTCTGCGGCGTCGACATCGCCGAGGTCGACCTCAACAGATGCGAGCCCTGGGAGCTCCCAGATGTGGCAAAGATGGGGGACAAAGAGTGGTACTTCTTCAGCCTCCGGGACCGGAAGTACCCAACCGGACTGAGAACCAACCGAGCCACCGGCGCCGGTTACTGGAAAGCCACCGGCAAAGACAAAGAGGTTTACAGCGCCTCAAGTGGAGCCCTCCTTGGGATGAAGAAGACCCTCGTCTTCTACAAAGGTCGAGCCCCCCGTGGCCAGAAGACCAAGTGGGTCATGCATGAGTACCGCCTCGACGGCGACTTCTCCTCCTACCGCCACACGTGCAAGGATGAATGGGTGATATGCAGAACTCATAACAAATCTGGGGACAAGAAGAACCCAATGCAAGCTCAAGGGCAAGGAGAAGGGCAACAGAGTAATTTTCTTCAACAAGTTGTGGAAGCTGCTTCGGCTTCTTCCAGAACTGGCTGCAGTTTGCCTCCATTGCTGGAATTTCAATCTCAGACGACCCATCAGAACCCTAGCTTTGTGCAAAGCCCTAGTTTACTGCGGATGCAAGAAAACGACTTGATGAAAAGTCGTAATCAACTGTTTCAACCACCGTCGTCGTCGTCGTCATCATCTTCATCCTCAGCAGCAGCTCTGCAGTCCATGCTCTTCAAGTCCTTCCTCTTTCCACATCAAGATCAAGCTGGCACTACTGGAACTGGAACTGGTGCTCCTAAACGGTGCAAGGCAGAAGCCACTAATCTTCCCCACTTCCAAACTGCACCTCTTAATGTTGCTAACTTCAACTACTTGATGGAAAAGAATCACTCTCATGTTCAGCCAAATGGCCAAGACTCGTTGCTTCAGTATCATCACCATCCCCGAAGCTCACTGTCGTTTGATGACTTCCATTTTTACAATGGAGTGTTGGGGCTTTCTACATCTGCTGCTACTGCTTCTGTAGATGCTGAGACCGCGTCGTCTTCTGCTTCAAACGGTGGTTTCAACAGGGCAGGCTTTCAGACCATGTTAGATCCTCTCATCAAACTCCCAGCTGCAGAATCATCATGGAATCAGGATATCGCCTAA

Protein Analysis

411

Amino Acids

45.41

Weight (kDa)

6.67

Isoelectric Point (pI)

39.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013567)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G76420
fragaria_vesca FvH4_5g25960 FvH4_5g25960 FvH4_5g25960
malus_domestica MD06G1004900.v1.1
prunus_persica Prupe.5G006200_v2.0.a1
pyrus_communis pycom06g00440 pycom16g26170
rosa_chinensis RchiOBHm_Chr7g0216521
rosa_laevigata RLG00000002564
rosa_multiflora Rmu_sc0009638.1_g000003 Rmu_sc0009638.1_g000004
rosa_roxburghii Rroxscaffold_3G00243140
rosa_rugosa Rorug07G0160700
rosa_samantha Rh7AG300000 Rh7BG291900 Rh7CG318600
rosa_wichuraiana Rw7G025510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1123
AccB1I GGYRCC 1 cut(s) 296
AccI GTMKAC 3 cut(s) 132, 156, 171
AccII CGCG 1 cut(s) 1121
AciI CCGC 5 cut(s) 150, 442, 469, 706, 1119
AclWI GGATC 1 cut(s) 1172
AcoI YGGCCR 2 cut(s) 409, 991
AcsI RAATTY 1 cut(s) 647
AcuI CTGAAG 2 cut(s) 226, 992
AcvI CACGTG 1 cut(s) 477
AcyI GRCGYC 2 cut(s) 153, 297
AfaI GTAC 3 cut(s) 236, 263, 440
AfiI CCNNNNNNNGG 3 cut(s) 208, 306, 363
AflIII ACRYGT 1 cut(s) 474
AgsI TTSAA 7 cut(s) 584, 653, 752, 817, 949, 1137, 1150
AjnI CCWGG 2 cut(s) 70, 191
AloI GAACNNNNNNTCC 2 cut(s) 859, 891
Alw21I GWGCWC 3 cut(s) 105, 201, 877
Alw26I GTCTC 1 cut(s) 1109
AlwI GGATC 1 cut(s) 1172
AoxI GGCC 2 cut(s) 409, 991
ApeKI GCWGC 7 cut(s) 31, 599, 624, 791, 794, 1091, 1199
ApoI RAATTY 1 cut(s) 647
AspLEI GCGC 3 cut(s) 143, 299, 344
AspS9I GGNCC 1 cut(s) 253
AsuC2I CCSGG 1 cut(s) 251
AsuHPI GGTGA 4 cut(s) 61, 100, 505, 1010
AvaII GGWCC 1 cut(s) 253
BalI TGGCCA 2 cut(s) 411, 993
BanI GGYRCC 1 cut(s) 296
BanII GRGCYC 5 cut(s) 105, 192, 201, 358, 403
BbrPI CACGTG 1 cut(s) 477
BbsI GAAGAC 5 cut(s) 56, 376, 380, 422, 1119
Bbv12I GWGCWC 3 cut(s) 105, 201, 877
BbvCI CCTCAGC 1 cut(s) 787
BbvI GCAGC 7 cut(s) 18, 586, 611, 803, 806, 1078, 1186
BccI CCATC 5 cut(s) 7, 211, 675, 952, 1029
BceAI ACGGC 1 cut(s) 466
BciT130I CCWGG 2 cut(s) 72, 193
BcnI CCSGG 1 cut(s) 251
BcoDI GTCTC 1 cut(s) 1109
BfaI CTAG 2 cut(s) 678, 696
BfmI CTRYAG 4 cut(s) 625, 800, 1104, 1200
BfoI RGCGCY 3 cut(s) 144, 300, 345
BglI GCCNNNNNGGC 1 cut(s) 450
BisI GCNGC 8 cut(s) 32, 151, 600, 625, 792, 795, 1092, 1200
BlsI GCNGC 8 cut(s) 33, 152, 601, 626, 793, 796, 1093, 1201
Bme1390I CCNGG 3 cut(s) 72, 193, 251
Bme18I GGWCC 1 cut(s) 253
BmgT120I GGNCC 1 cut(s) 253
BmiI GGNNCC 4 cut(s) 83, 254, 298, 355
BmrFI CCNGG 3 cut(s) 72, 193, 251
BmsI GCATC 3 cut(s) 173, 699, 1099
BoxI GACNNNNGTC 1 cut(s) 380
BpiI GAAGAC 5 cut(s) 56, 376, 380, 422, 1119
Bpu10I CCTNAGC 1 cut(s) 787
BpuEI CTTGAG 2 cut(s) 331, 531
BpuMI CCSGG 1 cut(s) 251
BsaAI YACGTR 1 cut(s) 477
BsaHI GRCGYC 2 cut(s) 153, 297
BsaI GGTCTC 1 cut(s) 1109
BsaJI CCNNGG 6 cut(s) 126, 165, 191, 192, 361, 406
BsaWI WCCGGW 2 cut(s) 255, 269
BsaXI ACNNNNNCTCC 4 cut(s) 446, 476, 859, 889
Bsc4I CCNNNNNNNGG 3 cut(s) 208, 306, 363
Bse118I RCCGGY 3 cut(s) 293, 299, 317
Bse1I ACTGG 5 cut(s) 311, 625, 862, 868, 874
Bse3DI GCAATG 1 cut(s) 638
BseBI CCWGG 2 cut(s) 72, 193
BseDI CCNNGG 6 cut(s) 126, 165, 191, 192, 361, 406
BseGI GGATG 5 cut(s) 372, 490, 714, 782, 1021
BseLI CCNNNNNNNGG 3 cut(s) 208, 306, 363
BseMI GCAATG 1 cut(s) 638
BseMII CTCAG 4 cut(s) 266, 671, 801, 1104
BseNI ACTGG 5 cut(s) 311, 625, 862, 868, 874
BseRI GAGGAG 2 cut(s) 113, 451
BseXI GCAGC 7 cut(s) 18, 586, 611, 803, 806, 1078, 1186
BseYI CCCAGC 2 cut(s) 34, 1195
BsgI GTGCAG 1 cut(s) 909
Bsh1236I CGCG 1 cut(s) 1121
BshFI GGCC 2 cut(s) 411, 993
BshNI GGYRCC 1 cut(s) 296
BsiHKAI GWGCWC 3 cut(s) 105, 201, 877
BsiSI CCGG 6 cut(s) 250, 256, 270, 294, 300, 318
BslFI GGGAC 3 cut(s) 236, 266, 536
BslI CCNNNNNNNGG 3 cut(s) 208, 306, 363
BsmAI GTCTC 1 cut(s) 1109
BsmFI GGGAC 3 cut(s) 236, 266, 536
BsnI GGCC 2 cut(s) 411, 993
Bso31I GGTCTC 1 cut(s) 1109
Bsp1286I GDGCHC 6 cut(s) 105, 192, 201, 358, 403, 877
Bsp143I GATC 2 cut(s) 841, 1177
BspACI CCGC 5 cut(s) 150, 442, 469, 706, 1119
BspANI GGCC 2 cut(s) 411, 993
BspCNI CTCAG 4 cut(s) 267, 670, 800, 1105
BspFNI CGCG 1 cut(s) 1121
BspLI GGNNCC 4 cut(s) 83, 254, 298, 355
BspMAI CTGCAG 3 cut(s) 629, 804, 1204
BspPI GGATC 1 cut(s) 1172
BspQI GCTCTTC 1 cut(s) 818
BspT107I GGYRCC 1 cut(s) 296
BspTNI GGTCTC 1 cut(s) 1109
BsrDI GCAATG 1 cut(s) 638
BsrFI RCCGGY 3 cut(s) 293, 299, 317
BsrI ACTGG 5 cut(s) 311, 625, 862, 868, 874
BssAI RCCGGY 3 cut(s) 293, 299, 317
BssECI CCNNGG 6 cut(s) 126, 165, 191, 192, 361, 406
BssMI GATC 2 cut(s) 841, 1177
BssNI GRCGYC 2 cut(s) 153, 297
BssT1I CCWWGG 2 cut(s) 126, 361
Bst2UI CCWGG 2 cut(s) 72, 193
Bst4CI ACNGT 5 cut(s) 747, 759, 885, 1038, 1142
Bst6I CTCTTC 2 cut(s) 39, 818
BstACI GRCGYC 2 cut(s) 153, 297
BstAPI GCANNNNNTGC 1 cut(s) 808
BstBAI YACGTR 1 cut(s) 477
BstC8I GCNNGC 4 cut(s) 188, 543, 850, 1159
BstDEI CTNAG 4 cut(s) 275, 657, 787, 1113
BstDSI CCRYGG 1 cut(s) 406
BstF5I GGATG 5 cut(s) 372, 490, 714, 782, 1021
BstFNI CGCG 1 cut(s) 1121
BstH2I RGCGCY 3 cut(s) 144, 300, 345
BstHHI GCGC 3 cut(s) 143, 299, 344
BstKTI GATC 2 cut(s) 844, 1180
BstMAI GTCTC 1 cut(s) 1109
BstMBI GATC 2 cut(s) 841, 1177
BstNI CCWGG 2 cut(s) 72, 193
BstPAI GACNNNNGTC 1 cut(s) 380
BstSCI CCNGG 3 cut(s) 70, 191, 249
BstSFI CTRYAG 4 cut(s) 625, 800, 1104, 1200
BstUI CGCG 1 cut(s) 1121
BstV1I GCAGC 7 cut(s) 18, 586, 611, 803, 806, 1078, 1186
BstV2I GAAGAC 5 cut(s) 56, 376, 380, 422, 1119
BstX2I RGATCY 1 cut(s) 1177
BstYI RGATCY 1 cut(s) 1177
BsuRI GGCC 2 cut(s) 411, 993
BtgI CCRYGG 1 cut(s) 406
BtgZI GCGATG 1 cut(s) 145
BtsCI GGATG 5 cut(s) 372, 490, 714, 782, 1021
BtsIMutI CAGTG 1 cut(s) 1034
Cac8I GCNNGC 4 cut(s) 188, 543, 850, 1159
CfoI GCGC 3 cut(s) 143, 299, 344
Cfr10I RCCGGY 3 cut(s) 293, 299, 317
Cfr13I GGNCC 1 cut(s) 253
CseI GACGC 2 cut(s) 142, 1110
CsiI ACCWGGT 1 cut(s) 70
Csp6I GTAC 3 cut(s) 235, 262, 439
CviAII CATG 6 cut(s) 430, 434, 808, 977, 1171, 1212
CviQI GTAC 3 cut(s) 235, 262, 439
DdeI CTNAG 4 cut(s) 275, 657, 787, 1113
DinI GGCGCC 1 cut(s) 298
DpnI GATC 2 cut(s) 843, 1179
DpnII GATC 2 cut(s) 841, 1177
DrdI GACNNNNNNGTC 1 cut(s) 1123
DseDI GACNNNNNNGTC 1 cut(s) 1123
EaeI YGGCCR 2 cut(s) 409, 991
Eam1104I CTCTTC 2 cut(s) 39, 818
EarI CTCTTC 2 cut(s) 39, 818
Ecl136II GAGCTC 2 cut(s) 103, 199
Eco130I CCWWGG 2 cut(s) 126, 361
Eco24I GRGCYC 5 cut(s) 105, 192, 201, 358, 403
Eco31I GGTCTC 1 cut(s) 1109
Eco32I GATATC 1 cut(s) 1225
Eco47I GGWCC 1 cut(s) 253
Eco53kI GAGCTC 2 cut(s) 103, 199
Eco57I CTGAAG 2 cut(s) 226, 992
Eco72I CACGTG 1 cut(s) 477
EcoICRI GAGCTC 2 cut(s) 103, 199
EcoRII CCWGG 2 cut(s) 70, 191
EcoRV GATATC 1 cut(s) 1225
EcoT14I CCWWGG 2 cut(s) 126, 361
EcoT22I ATGCAT 1 cut(s) 435
EcoT38I GRGCYC 5 cut(s) 105, 192, 201, 358, 403
EgeI GGCGCC 1 cut(s) 298
EheI GGCGCC 1 cut(s) 298
ErhI CCWWGG 2 cut(s) 126, 361
FaeI CATG 6 cut(s) 433, 437, 811, 980, 1174, 1215
FaiI YATR 8 cut(s) 431, 435, 499, 510, 809, 978, 1172, 1213
FaqI GGGAC 3 cut(s) 236, 266, 536
FatI CATG 6 cut(s) 429, 433, 807, 976, 1170, 1211
FblI GTMKAC 3 cut(s) 132, 156, 171
Fnu4HI GCNGC 8 cut(s) 32, 151, 600, 625, 792, 795, 1092, 1200
FokI GGATG 5 cut(s) 379, 497, 721, 769, 1008
FriOI GRGCYC 5 cut(s) 105, 192, 201, 358, 403
Fsp4HI GCNGC 8 cut(s) 32, 151, 600, 625, 792, 795, 1092, 1200
FspBI CTAG 2 cut(s) 678, 696
GlaI GCGC 3 cut(s) 142, 298, 343
GluI GCNGC 8 cut(s) 32, 151, 600, 625, 792, 795, 1092, 1200
GsaI CCCAGC 2 cut(s) 38, 1199
HaeII RGCGCY 3 cut(s) 144, 300, 345
HaeIII GGCC 2 cut(s) 411, 993
HapII CCGG 6 cut(s) 250, 256, 270, 294, 300, 318
HgaI GACGC 2 cut(s) 142, 1110
HhaI GCGC 3 cut(s) 143, 299, 344
Hin1I GRCGYC 2 cut(s) 153, 297
Hin1II CATG 6 cut(s) 433, 437, 811, 980, 1174, 1215
Hin6I GCGC 3 cut(s) 141, 297, 342
HinP1I GCGC 3 cut(s) 141, 297, 342
HincII GTYRAC 2 cut(s) 157, 172
HindII GTYRAC 2 cut(s) 157, 172
HinfI GANTC 4 cut(s) 967, 998, 1205, 1216
HpaII CCGG 6 cut(s) 250, 256, 270, 294, 300, 318
HphI GGTGA 4 cut(s) 61, 100, 505, 1010
Hpy166II GTNNAC 6 cut(s) 52, 133, 157, 172, 337, 701
Hpy188I TCNGA 3 cut(s) 660, 672, 1167
Hpy188III TCNNGA 3 cut(s) 615, 839, 1220
Hpy8I GTNNAC 6 cut(s) 52, 133, 157, 172, 337, 701
Hpy99I CGWCG 7 cut(s) 158, 452, 763, 766, 769, 772, 1126
HpyAV CCTTC 2 cut(s) 554, 832
HpyCH4III ACNGT 5 cut(s) 747, 759, 885, 1038, 1142
HpyCH4IV ACGT 1 cut(s) 476
HpyF3I CTNAG 4 cut(s) 275, 657, 787, 1113
HpySE526I ACGT 1 cut(s) 476
Hsp92I GRCGYC 2 cut(s) 153, 297
Hsp92II CATG 6 cut(s) 433, 437, 811, 980, 1174, 1215
HspAI GCGC 3 cut(s) 141, 297, 342
KasI GGCGCC 1 cut(s) 296
Kzo9I GATC 2 cut(s) 841, 1177
LguI GCTCTTC 1 cut(s) 818
LmnI GCTCC 5 cut(s) 100, 196, 204, 353, 880
Lsp1109I GCAGC 7 cut(s) 18, 586, 611, 803, 806, 1078, 1186
LweI GCATC 3 cut(s) 173, 699, 1099
MabI ACCWGGT 1 cut(s) 70
MaeI CTAG 2 cut(s) 678, 696
MaeII ACGT 1 cut(s) 476
MaeIII GTNAC 1 cut(s) 302
MalI GATC 2 cut(s) 843, 1179
MboI GATC 2 cut(s) 841, 1177
MflI RGATCY 1 cut(s) 1177
MhlI GDGCHC 6 cut(s) 105, 192, 201, 358, 403, 877
MlsI TGGCCA 2 cut(s) 411, 993
MluCI AATT 2 cut(s) 574, 647
MluNI TGGCCA 2 cut(s) 411, 993
Mly113I GGCGCC 1 cut(s) 297
MlyI GAGTC 1 cut(s) 992
Mox20I TGGCCA 2 cut(s) 411, 993
Mph1103I ATGCAT 1 cut(s) 435
MscI TGGCCA 2 cut(s) 411, 993
MseI TTAA 1 cut(s) 933
MslI CAYNNNNRTG 1 cut(s) 975
Msp20I TGGCCA 2 cut(s) 411, 993
MspA1I CMGCKG 1 cut(s) 1199
MspI CCGG 6 cut(s) 250, 256, 270, 294, 300, 318
MspR9I CCNGG 3 cut(s) 72, 193, 251
MvaI CCWGG 2 cut(s) 72, 193
MvnI CGCG 1 cut(s) 1121
NarI GGCGCC 1 cut(s) 297
NciI CCSGG 1 cut(s) 251
NdeII GATC 2 cut(s) 841, 1177
NlaIII CATG 6 cut(s) 433, 437, 811, 980, 1174, 1215
NlaIV GGNNCC 4 cut(s) 83, 254, 298, 355
NmeAIII GCCGAG 1 cut(s) 190
NsiI ATGCAT 1 cut(s) 435
PasI CCCWGGG 1 cut(s) 192
PciSI GCTCTTC 1 cut(s) 818
PcsI WCGNNNNNNNCGW 4 cut(s) 162, 168, 403, 767
PfeI GAWTC 3 cut(s) 967, 1205, 1216
PflFI GACNNNGTC 1 cut(s) 1120
PfoI TCCNGGA 1 cut(s) 249
PkrI GCNGC 8 cut(s) 33, 152, 601, 626, 793, 796, 1093, 1201
PleI GAGTC 1 cut(s) 992
PluTI GGCGCC 1 cut(s) 300
PmaCI CACGTG 1 cut(s) 477
PmlI CACGTG 1 cut(s) 477
PpsI GAGTC 1 cut(s) 992
Ppu21I YACGTR 1 cut(s) 477
PshAI GACNNNNGTC 1 cut(s) 380
Psp124BI GAGCTC 2 cut(s) 105, 201
Psp6I CCWGG 2 cut(s) 70, 191
PspCI CACGTG 1 cut(s) 477
PspFI CCCAGC 2 cut(s) 34, 1195
PspGI CCWGG 2 cut(s) 70, 191
PspN4I GGNNCC 4 cut(s) 83, 254, 298, 355
PspPI GGNCC 1 cut(s) 253
PstI CTGCAG 3 cut(s) 629, 804, 1204
PsuI RGATCY 1 cut(s) 1177
PsyI GACNNNGTC 1 cut(s) 1120
PvuII CAGCTG 1 cut(s) 1199
RsaI GTAC 3 cut(s) 236, 263, 440
RsaNI GTAC 3 cut(s) 235, 262, 439
RseI CAYNNNNRTG 1 cut(s) 975
SacI GAGCTC 2 cut(s) 105, 201
SalI GTCGAC 2 cut(s) 155, 170
SapI GCTCTTC 1 cut(s) 818
SaqAI TTAA 1 cut(s) 933
SatI GCNGC 8 cut(s) 32, 151, 600, 625, 792, 795, 1092, 1200
Sau3AI GATC 2 cut(s) 841, 1177
Sau96I GGNCC 1 cut(s) 253
SchI GAGTC 1 cut(s) 992
ScrFI CCNGG 3 cut(s) 72, 193, 251
SduI GDGCHC 6 cut(s) 105, 192, 201, 358, 403, 877
SexAI ACCWGGT 1 cut(s) 70
SfaNI GCATC 3 cut(s) 173, 699, 1099
SfcI CTRYAG 4 cut(s) 625, 800, 1104, 1200
SfoI GGCGCC 1 cut(s) 298
SgrAI CRCCGGYG 1 cut(s) 293
SinI GGWCC 1 cut(s) 253
SmiMI CAYNNNNRTG 1 cut(s) 975
SmlI CTYRAG 2 cut(s) 346, 546
SmoI CTYRAG 2 cut(s) 346, 546
Sse9I AATT 2 cut(s) 574, 647
SsiI CCGC 5 cut(s) 150, 442, 469, 706, 1119
SspDI GGCGCC 1 cut(s) 296
SspMI CTAG 2 cut(s) 678, 696
SstI GAGCTC 2 cut(s) 105, 201
StyD4I CCNGG 3 cut(s) 70, 191, 249
StyI CCWWGG 2 cut(s) 126, 361
TaaI ACNGT 5 cut(s) 747, 759, 885, 1038, 1142
TaiI ACGT 1 cut(s) 479
TaqI TCGA 4 cut(s) 156, 171, 397, 447
TasI AATT 2 cut(s) 574, 647
TauI GCSGC 1 cut(s) 153
TfiI GAWTC 3 cut(s) 967, 1205, 1216
Tru1I TTAA 1 cut(s) 933
Tru9I TTAA 1 cut(s) 933
TscAI CASTG 1 cut(s) 1041
TseI GCWGC 7 cut(s) 31, 599, 624, 791, 794, 1091, 1199
TspDTI ATGAA 4 cut(s) 383, 501, 743, 771
TspRI CASTG 1 cut(s) 1041
Tth111I GACNNNGTC 1 cut(s) 1120
VpaK11BI GGWCC 1 cut(s) 253
XapI RAATTY 1 cut(s) 647
XcmI CCANNNNNNNNNTGG 1 cut(s) 1061
XmiI GTMKAC 3 cut(s) 132, 156, 171
XspI CTAG 2 cut(s) 678, 696
Zsp2I ATGCAT 1 cut(s) 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.