pycom06g00440

Protein CUP-SHAPED COTYLEDON

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0013567
Physical Location & Seq
Forward (+)
429027 .. 432143
3117 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g00440.2

Sequence Viewer

Length: 1011 bp
ATGTTGGGAATGGGAATGGAAGAAGTTATGAGTGAGCTGATGAGTGGGGAGGATGAAAACGGACAAGGTTTTCAACCAGGGTTCCGGTTCCATCCGACGGACGAGGAGCTCATAACCTTTTATCTGGCTTCCAAGGTCTACAACGGCTCTTTCCGCGGCGTCCGCATTGCTGAGGTCGACCTTAACCGATGCGAACCATGGGAGCTGCCAGACGTGGCAAAGATGGGAGAGAGAGAGTGGTACTTCTTCAGCCTGAGGGACAGGAAGTACCCAACGGGGATGAGAACCAACAGAGCGACCGGAGCCGGCTACTGGAAGGCCACCGGCAAAGACAGACAGGTCTTCAGTGCGTCGACCGGAGCCCTACTTGGCATGAAGAAGACCCTTGTCTTCTACAAGGGCAGAGCTCCGAGGGGCCACAAGACTAAGTGGGTCATGCATGAGTACCGTCTCGACGGCCACTTCTCGTCCTATCGCCACGACTGCCAGGAGGAATGGGTGATATGCAGAATAAATCACAAATCCGGGAAGAAGAAAAGCCCACTACTTGATCACGAAGCTGCTGCTTCAGCAATTTCAAATTGCAATTATCTGCCTCCATTGCTGGATCCATCTCCAGCATTATTATTGCAAGAATCATGCCATGAATCTCAGAATCCAATACAATCGCAAGCTCTTCCTCCAATTCACCAAGAAAATCATGATACCTTCTCCTCTTTCCCAGCAATAAATGCCTTCCAAACCCACCAATTACCCTCTTTCTCCAGATCCCTCCTCTTCAAATCCCTCTTCGATCAAGATTGCAACAGCAACAGTAGTTCGAGTACTGTTCCCAAACAGTGCAAGACTGAGCTCGCCAACTACTTCTCCCGTTTCCAAACACCGGATAACAGTCATAACAATAATAATAGCACTCGCAACAACTTGCATGACTTGAACTTTGTGGTGGAATTGGAAAAGAATCATGATCGATCAGTACCAGCCAAACCCCCCGTACCATCATCTTCATGA

Protein Analysis

337

Amino Acids

38.19

Weight (kDa)

7.23

Isoelectric Point (pI)

46.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013567)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G76420
fragaria_vesca FvH4_5g25960 FvH4_5g25960 FvH4_5g25960
malus_domestica MD06G1004900.v1.1
prunus_persica Prupe.5G006200_v2.0.a1
pyrus_communis pycom06g00440 pycom16g26170
rosa_chinensis RchiOBHm_Chr7g0216521
rosa_laevigata RLG00000002564
rosa_multiflora Rmu_sc0009638.1_g000003 Rmu_sc0009638.1_g000004
rosa_roxburghii Rroxscaffold_3G00243140
rosa_rugosa Rorug07G0160700
rosa_samantha Rh7AG300000 Rh7BG291900 Rh7CG318600
rosa_wichuraiana Rw7G025510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 338
AccI GTMKAC 3 cut(s) 138, 177, 353
AccII CGCG 1 cut(s) 156
AciI CCGC 3 cut(s) 154, 156, 163
AclWI GGATC 3 cut(s) 602, 615, 762
AcoI YGGCCR 1 cut(s) 457
AcuI CTGAAG 3 cut(s) 232, 328, 552
AcyI GRCGYC 1 cut(s) 159
AfaI GTAC 6 cut(s) 242, 269, 446, 826, 978, 996
AfiI CCNNNNNNNGG 3 cut(s) 97, 312, 883
AgsI TTSAA 4 cut(s) 74, 579, 781, 937
AjiI CACGTC 1 cut(s) 214
AjnI CCWGG 2 cut(s) 76, 486
AluBI AGCT 7 cut(s) 37, 109, 205, 407, 560, 674, 853
AluI AGCT 7 cut(s) 37, 109, 205, 407, 560, 674, 853
Alw21I GWGCWC 3 cut(s) 111, 409, 855
Alw26I GTCTC 1 cut(s) 455
AlwI GGATC 3 cut(s) 602, 615, 762
AoxI GGCC 3 cut(s) 318, 415, 457
ApeKI GCWGC 3 cut(s) 205, 560, 563
ArsI GACNNNNNNTTYG 2 cut(s) 923, 955
AspS9I GGNCC 1 cut(s) 415
AsuC2I CCSGG 1 cut(s) 526
AsuHPI GGTGA 2 cut(s) 511, 680
AxyI CCTNAGG 1 cut(s) 254
BamHI GGATCC 1 cut(s) 607
BanII GRGCYC 4 cut(s) 111, 364, 409, 855
BbsI GAAGAC 3 cut(s) 334, 382, 386
Bbv12I GWGCWC 3 cut(s) 111, 409, 855
BbvCI CCTCAGC 1 cut(s) 171
BbvI GCAGC 3 cut(s) 192, 547, 550
BccI CCATC 4 cut(s) 99, 217, 619, 1006
BceAI ACGGC 2 cut(s) 160, 472
BcgI CGANNNNNNTGC 2 cut(s) 545, 579
BciT130I CCWGG 2 cut(s) 78, 488
BclI TGATCA 1 cut(s) 550
BcnI CCSGG 1 cut(s) 526
BcoDI GTCTC 1 cut(s) 455
BisI GCNGC 4 cut(s) 157, 206, 561, 564
BlsI GCNGC 4 cut(s) 158, 207, 562, 565
BmcAI AGTACT 1 cut(s) 826
Bme1390I CCNGG 3 cut(s) 78, 488, 526
BmgBI CACGTC 1 cut(s) 214
BmgT120I GGNCC 1 cut(s) 415
BmiI GGNNCC 6 cut(s) 83, 89, 304, 361, 416, 609
BmrFI CCNGG 3 cut(s) 78, 488, 526
BmsI GCATC 1 cut(s) 179
BoxI GACNNNNGTC 1 cut(s) 386
BpiI GAAGAC 3 cut(s) 334, 382, 386
BpmI CTGGAG 2 cut(s) 600, 748
Bpu10I CCTNAGC 1 cut(s) 171
BpuMI CCSGG 1 cut(s) 526
Bsa29I ATCGAT 1 cut(s) 970
BsaHI GRCGYC 1 cut(s) 159
BsaJI CCNNGG 5 cut(s) 77, 132, 154, 197, 410
BsaWI WCCGGW 4 cut(s) 84, 299, 356, 883
BsaXI ACNNNNNCTCC 2 cut(s) 851, 881
Bsc4I CCNNNNNNNGG 3 cut(s) 97, 312, 883
Bse118I RCCGGY 2 cut(s) 305, 323
Bse1I ACTGG 1 cut(s) 317
Bse21I CCTNAGG 1 cut(s) 254
Bse3DI GCAATG 2 cut(s) 165, 599
BseBI CCWGG 2 cut(s) 78, 488
BseCI ATCGAT 1 cut(s) 970
BseDI CCNNGG 5 cut(s) 77, 132, 154, 197, 410
BseGI GGATG 3 cut(s) 58, 91, 285
BseLI CCNNNNNNNGG 3 cut(s) 97, 312, 883
BseMI GCAATG 2 cut(s) 165, 599
BseMII CTCAG 4 cut(s) 162, 245, 665, 840
BseNI ACTGG 1 cut(s) 317
BseRI GAGGAG 3 cut(s) 119, 703, 764
BseXI GCAGC 3 cut(s) 192, 547, 550
BseYI CCCAGC 1 cut(s) 721
Bsh1236I CGCG 1 cut(s) 156
Bsh1285I CGRYCG 2 cut(s) 300, 357
BshFI GGCC 3 cut(s) 320, 417, 459
BshVI ATCGAT 1 cut(s) 970
BsiEI CGRYCG 2 cut(s) 300, 357
BsiHKAI GWGCWC 3 cut(s) 111, 409, 855
BsiSI CCGG 7 cut(s) 85, 300, 306, 324, 357, 525, 884
BslFI GGGAC 1 cut(s) 272
BslI CCNNNNNNNGG 3 cut(s) 97, 312, 883
BsmAI GTCTC 1 cut(s) 455
BsmBI CGTCTC 1 cut(s) 455
BsmFI GGGAC 1 cut(s) 272
BsnI GGCC 3 cut(s) 320, 417, 459
Bsp1286I GDGCHC 4 cut(s) 111, 364, 409, 855
Bsp143I GATC 6 cut(s) 550, 607, 767, 793, 967, 971
Bsp19I CCATGG 1 cut(s) 197
BspACI CCGC 3 cut(s) 154, 156, 163
BspANI GGCC 3 cut(s) 320, 417, 459
BspCNI CTCAG 4 cut(s) 163, 246, 664, 841
BspDI ATCGAT 1 cut(s) 970
BspFNI CGCG 1 cut(s) 156
BspHI TCATGA 3 cut(s) 700, 964, 1007
BspLI GGNNCC 6 cut(s) 83, 89, 304, 361, 416, 609
BspPI GGATC 3 cut(s) 602, 615, 762
BspQI GCTCTTC 1 cut(s) 681
BsrDI GCAATG 2 cut(s) 165, 599
BsrFI RCCGGY 2 cut(s) 305, 323
BsrI ACTGG 1 cut(s) 317
BssAI RCCGGY 2 cut(s) 305, 323
BssECI CCNNGG 5 cut(s) 77, 132, 154, 197, 410
BssMI GATC 6 cut(s) 550, 607, 767, 793, 967, 971
BssNI GRCGYC 1 cut(s) 159
BssT1I CCWWGG 2 cut(s) 132, 197
Bst2UI CCWGG 2 cut(s) 78, 488
Bst4CI ACNGT 5 cut(s) 449, 815, 829, 840, 893
Bst6I CTCTTC 3 cut(s) 681, 782, 794
BstACI GRCGYC 1 cut(s) 159
BstAPI GCANNNNNTGC 1 cut(s) 731
BstC8I GCNNGC 3 cut(s) 307, 672, 855
BstDEI CTNAG 5 cut(s) 171, 254, 426, 651, 849
BstDSI CCRYGG 2 cut(s) 154, 197
BstF5I GGATG 3 cut(s) 58, 91, 285
BstFNI CGCG 1 cut(s) 156
BstKTI GATC 6 cut(s) 553, 610, 770, 796, 970, 974
BstMAI GTCTC 1 cut(s) 455
BstMBI GATC 6 cut(s) 550, 607, 767, 793, 967, 971
BstMCI CGRYCG 2 cut(s) 300, 357
BstMWI GCNNNNNNNGC 7 cut(s) 153, 162, 302, 483, 569, 601, 731
BstNI CCWGG 2 cut(s) 78, 488
BstPAI GACNNNNGTC 1 cut(s) 386
BstSCI CCNGG 3 cut(s) 76, 486, 524
BstUI CGCG 1 cut(s) 156
BstV1I GCAGC 3 cut(s) 192, 547, 550
BstV2I GAAGAC 3 cut(s) 334, 382, 386
BstX2I RGATCY 2 cut(s) 607, 767
BstYI RGATCY 2 cut(s) 607, 767
Bsu15I ATCGAT 1 cut(s) 970
Bsu36I CCTNAGG 1 cut(s) 254
BsuRI GGCC 3 cut(s) 320, 417, 459
BsuTUI ATCGAT 1 cut(s) 970
BtgI CCRYGG 2 cut(s) 154, 197
BtrI CACGTC 1 cut(s) 214
BtsCI GGATG 3 cut(s) 58, 91, 285
BtsIMutI CAGTG 2 cut(s) 352, 845
Cac8I GCNNGC 3 cut(s) 307, 672, 855
CciI TCATGA 3 cut(s) 700, 964, 1007
Cfr10I RCCGGY 2 cut(s) 305, 323
Cfr13I GGNCC 1 cut(s) 415
Cfr42I CCGCGG 1 cut(s) 157
ClaI ATCGAT 1 cut(s) 970
CseI GACGC 2 cut(s) 148, 339
Csp6I GTAC 6 cut(s) 241, 268, 445, 825, 977, 995
CviQI GTAC 6 cut(s) 241, 268, 445, 825, 977, 995
DdeI CTNAG 5 cut(s) 171, 254, 426, 651, 849
DpnI GATC 6 cut(s) 552, 609, 769, 795, 969, 973
DpnII GATC 6 cut(s) 550, 607, 767, 793, 967, 971
DrdI GACNNNNNNGTC 1 cut(s) 338
DseDI GACNNNNNNGTC 1 cut(s) 338
EaeI YGGCCR 1 cut(s) 457
Eam1104I CTCTTC 3 cut(s) 681, 782, 794
EarI CTCTTC 3 cut(s) 681, 782, 794
Ecl136II GAGCTC 3 cut(s) 109, 407, 853
Eco130I CCWWGG 2 cut(s) 132, 197
Eco24I GRGCYC 4 cut(s) 111, 364, 409, 855
Eco53kI GAGCTC 3 cut(s) 109, 407, 853
Eco57I CTGAAG 3 cut(s) 232, 328, 552
Eco81I CCTNAGG 1 cut(s) 254
EcoICRI GAGCTC 3 cut(s) 109, 407, 853
EcoRII CCWGG 2 cut(s) 76, 486
EcoT14I CCWWGG 2 cut(s) 132, 197
EcoT22I ATGCAT 1 cut(s) 441
EcoT38I GRGCYC 4 cut(s) 111, 364, 409, 855
ErhI CCWWGG 2 cut(s) 132, 197
Esp3I CGTCTC 1 cut(s) 455
FaqI GGGAC 1 cut(s) 272
FbaI TGATCA 1 cut(s) 550
FblI GTMKAC 3 cut(s) 138, 177, 353
Fnu4HI GCNGC 4 cut(s) 157, 206, 561, 564
FokI GGATG 3 cut(s) 65, 78, 292
FriOI GRGCYC 4 cut(s) 111, 364, 409, 855
Fsp4HI GCNGC 4 cut(s) 157, 206, 561, 564
GluI GCNGC 4 cut(s) 157, 206, 561, 564
GsaI CCCAGC 1 cut(s) 725
GsuI CTGGAG 2 cut(s) 600, 748
HaeIII GGCC 3 cut(s) 320, 417, 459
HapII CCGG 7 cut(s) 85, 300, 306, 324, 357, 525, 884
HgaI GACGC 2 cut(s) 148, 339
Hin1I GRCGYC 1 cut(s) 159
HincII GTYRAC 2 cut(s) 178, 354
HindII GTYRAC 2 cut(s) 178, 354
HinfI GANTC 4 cut(s) 635, 647, 655, 961
HpaII CCGG 7 cut(s) 85, 300, 306, 324, 357, 525, 884
HphI GGTGA 2 cut(s) 511, 680
Hpy166II GTNNAC 3 cut(s) 139, 178, 354
Hpy188I TCNGA 3 cut(s) 96, 411, 654
Hpy188III TCNNGA 7 cut(s) 452, 554, 701, 765, 797, 965, 1008
Hpy8I GTNNAC 3 cut(s) 139, 178, 354
Hpy99I CGWCG 3 cut(s) 100, 355, 458
HpyAV CCTTC 3 cut(s) 310, 718, 745
HpyCH4III ACNGT 5 cut(s) 449, 815, 829, 840, 893
HpyCH4IV ACGT 1 cut(s) 213
HpyCH4V TGCA 7 cut(s) 439, 507, 585, 631, 804, 843, 928
HpyF10VI GCNNNNNNNGC 7 cut(s) 153, 162, 302, 483, 569, 601, 731
HpyF3I CTNAG 5 cut(s) 171, 254, 426, 651, 849
HpySE526I ACGT 1 cut(s) 213
Hsp92I GRCGYC 1 cut(s) 159
KroI GCCGGC 1 cut(s) 305
KroNI GCCGGC 1 cut(s) 307
Ksp22I TGATCA 1 cut(s) 550
KspI CCGCGG 1 cut(s) 157
Kzo9I GATC 6 cut(s) 550, 607, 767, 793, 967, 971
LguI GCTCTTC 1 cut(s) 681
LmnI GCTCC 5 cut(s) 106, 202, 302, 359, 412
Lsp1109I GCAGC 3 cut(s) 192, 547, 550
LweI GCATC 1 cut(s) 179
MaeII ACGT 1 cut(s) 213
MalI GATC 6 cut(s) 552, 609, 769, 795, 969, 973
MboI GATC 6 cut(s) 550, 607, 767, 793, 967, 971
MflI RGATCY 2 cut(s) 607, 767
MhlI GDGCHC 4 cut(s) 111, 364, 409, 855
MluCI AATT 6 cut(s) 573, 580, 586, 684, 749, 950
MmeI TCCRAC 1 cut(s) 119
Mph1103I ATGCAT 1 cut(s) 441
MroNI GCCGGC 1 cut(s) 305
MseI TTAA 1 cut(s) 183
MslI CAYNNNNRTG 1 cut(s) 1006
MspA1I CMGCKG 1 cut(s) 156
MspI CCGG 7 cut(s) 85, 300, 306, 324, 357, 525, 884
MspR9I CCNGG 3 cut(s) 78, 488, 526
MvaI CCWGG 2 cut(s) 78, 488
MvnI CGCG 1 cut(s) 156
MwoI GCNNNNNNNGC 7 cut(s) 153, 162, 302, 483, 569, 601, 731
NaeI GCCGGC 1 cut(s) 307
NciI CCSGG 1 cut(s) 526
NcoI CCATGG 1 cut(s) 197
NdeII GATC 6 cut(s) 550, 607, 767, 793, 967, 971
NgoMIV GCCGGC 1 cut(s) 305
NlaIV GGNNCC 6 cut(s) 83, 89, 304, 361, 416, 609
NsiI ATGCAT 1 cut(s) 441
PagI TCATGA 3 cut(s) 700, 964, 1007
PciSI GCTCTTC 1 cut(s) 681
PdiI GCCGGC 1 cut(s) 307
PfeI GAWTC 4 cut(s) 635, 647, 655, 961
PfoI TCCNGGA 1 cut(s) 524
PkrI GCNGC 4 cut(s) 158, 207, 562, 565
PshAI GACNNNNGTC 1 cut(s) 386
Psp124BI GAGCTC 3 cut(s) 111, 409, 855
Psp6I CCWGG 2 cut(s) 76, 486
PspFI CCCAGC 1 cut(s) 721
PspGI CCWGG 2 cut(s) 76, 486
PspN4I GGNNCC 6 cut(s) 83, 89, 304, 361, 416, 609
PspPI GGNCC 1 cut(s) 415
PsuI RGATCY 2 cut(s) 607, 767
RsaI GTAC 6 cut(s) 242, 269, 446, 826, 978, 996
RsaNI GTAC 6 cut(s) 241, 268, 445, 825, 977, 995
RseI CAYNNNNRTG 1 cut(s) 1006
SacI GAGCTC 3 cut(s) 111, 409, 855
SacII CCGCGG 1 cut(s) 157
SalI GTCGAC 2 cut(s) 176, 352
SapI GCTCTTC 1 cut(s) 681
SaqAI TTAA 1 cut(s) 183
SatI GCNGC 4 cut(s) 157, 206, 561, 564
Sau3AI GATC 6 cut(s) 550, 607, 767, 793, 967, 971
Sau96I GGNCC 1 cut(s) 415
ScaI AGTACT 1 cut(s) 826
ScrFI CCNGG 3 cut(s) 78, 488, 526
SduI GDGCHC 4 cut(s) 111, 364, 409, 855
SfaNI GCATC 1 cut(s) 179
Sfr303I CCGCGG 1 cut(s) 157
SgrBI CCGCGG 1 cut(s) 157
SmiMI CAYNNNNRTG 1 cut(s) 1006
Sse9I AATT 6 cut(s) 573, 580, 586, 684, 749, 950
SsiI CCGC 3 cut(s) 154, 156, 163
SstI GAGCTC 3 cut(s) 111, 409, 855
StyD4I CCNGG 3 cut(s) 76, 486, 524
StyI CCWWGG 2 cut(s) 132, 197
TaaI ACNGT 5 cut(s) 449, 815, 829, 840, 893
TaiI ACGT 1 cut(s) 216
TaqI TCGA 6 cut(s) 177, 353, 453, 792, 821, 970
TasI AATT 6 cut(s) 573, 580, 586, 684, 749, 950
TatI WGTACW 1 cut(s) 824
TauI GCSGC 1 cut(s) 159
TfiI GAWTC 4 cut(s) 635, 647, 655, 961
Tru1I TTAA 1 cut(s) 183
Tru9I TTAA 1 cut(s) 183
TscAI CASTG 2 cut(s) 352, 845
TseI GCWGC 3 cut(s) 205, 560, 563
TspDTI ATGAA 4 cut(s) 69, 389, 660, 996
TspGWI ACGGA 2 cut(s) 75, 113
TspRI CASTG 2 cut(s) 352, 845
XmiI GTMKAC 3 cut(s) 138, 177, 353
ZrmI AGTACT 1 cut(s) 826
Zsp2I ATGCAT 1 cut(s) 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.