Rw7G025510

Protein CUP-SHAPED COTYLEDON

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0013567
Physical Location & Seq
Reverse (-)
31879378 .. 31881643
2266 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G025510.1

Sequence Viewer

Length: 1224 bp
ATGTTGGGTTTAATGGAAGAAGTTATGAGTGAGCTGATGGGTAGGGAAGAGCTGAACGAACAAGGTCTTCCACCTGGTTTCAGGTTCCACCCAACTGATGAGGAGCTCATCACCTTTTACCTCGCTTCCAAGGTCTACAATGGCGCTTTCTGCGGCGTCGACATCGCTGAGGTCGATCTCAACAGATGTGAGCCCTGGGAGCTACCAGATGTGGCGAAGATGGGGGACAGAGAGTGGTACTTCTTCAGCCTCCGGGACCGGAAGTACCCGACCGGCCTTAGGACCAACCGAGCCACCGGCGCTGGCTACTGGAAAGCCACCGGCAAAGACAAAGAGGTTTACAGTGCCTCCAGTGGGGCCCTTCTTGGGATGAAGAAGACCCTCGTCTTCTACAAGGGCCGTGCCCCCCGCGGCCAGAAGACCAAGTGGGTCATGCACGAGTACCGCCTCGACGGGGACTTCTCCTCCTACCGCCACACGTGCAAGGATGAATGGGTGATATGTAGAATACATAACAAATCTGGGGACAAAAAAAACCCACTGCAAGCTCAAGGGCAAGGGGAAGGGCAACAGAGTAATTTCCTGCAACAAGTTGTGGAAGCTGCTTCTGCTTCTGCTTCTTCCAAAACTGGCTGCTTGCCTCCATTGCTGGATGTCAATCTCAGACGACTCATCAGAACCCTAATTCAAGAAAACGACTTGATGAAAAGTCGTAATCAACTGTTTCAACAACCCTCATTATCATCATCATCTTCATCCTCAGCAGCAGCACTGCACTCCATGCTCTTCAAGTCACTCCTCTATTCACATCAAGATCAAGCTGGCACTGGTACTAGTGCTCCTAAACAGTGCAAGGCAGAGGCTGCTAATCTTTCCCAGTTCCAAACCCCACCTCTTAATGCTGCTAACTTAAACTACTTGATGGAAAAGAATCACTCTCATCATCAACCAAACGTCCCAGACTCATTGCTTCAGTTTCATCACCATCCCCGAAATGCACTCTCTTTCGATGACTTCCATTATTACAATGGTGTGTTGGGGCTTTCTACATCTGCTACTGCTGCTGTAGATGCTGAGACTTCTTCCGCTTCCGTTGGTGGTTTCAACAGGGCAGGCTTTCAGACCATGTTAGATCCTCTCATCAAACTCCCTGCTGCAGAATCTTGGAATCTGGATATCGTTAAGAATCCATATCATCGATTATCTGAATATATACATATATAG

Protein Analysis

407

Amino Acids

45.51

Weight (kDa)

7.25

Isoelectric Point (pI)

36.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 24 - 150 1.2e-46 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013567)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G76420
fragaria_vesca FvH4_5g25960 FvH4_5g25960 FvH4_5g25960
malus_domestica MD06G1004900.v1.1
prunus_persica Prupe.5G006200_v2.0.a1
pyrus_communis pycom06g00440 pycom16g26170
rosa_chinensis RchiOBHm_Chr7g0216521
rosa_laevigata RLG00000002564
rosa_multiflora Rmu_sc0009638.1_g000003 Rmu_sc0009638.1_g000004
rosa_roxburghii Rroxscaffold_3G00243140
rosa_rugosa Rorug07G0160700
rosa_samantha Rh7AG300000 Rh7BG291900 Rh7CG318600
rosa_wichuraiana Rw7G025510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 135, 159
AccII CGCG 1 cut(s) 411
AciI CCGC 6 cut(s) 153, 409, 411, 445, 472, 1086
AclWI GGATC 1 cut(s) 1127
AcoI YGGCCR 1 cut(s) 412
AcuI CTGAAG 2 cut(s) 229, 956
AcvI CACGTG 1 cut(s) 480
AcyI GRCGYC 1 cut(s) 156
AfaI GTAC 4 cut(s) 239, 266, 443, 832
AfiI CCNNNNNNNGG 4 cut(s) 279, 354, 366, 454
AflIII ACRYGT 1 cut(s) 477
AgsI TTSAA 4 cut(s) 689, 728, 790, 1105
AhlI ACTAGT 1 cut(s) 833
AjnI CCWGG 2 cut(s) 73, 194
AluBI AGCT 7 cut(s) 34, 52, 106, 202, 548, 602, 821
AluI AGCT 7 cut(s) 34, 52, 106, 202, 548, 602, 821
Alw21I GWGCWC 2 cut(s) 108, 841
Alw26I GTCTC 1 cut(s) 1070
AlwI GGATC 1 cut(s) 1127
AlwNI CAGNNNCTG 1 cut(s) 863
AoxI GGCC 4 cut(s) 274, 357, 397, 412
ApaI GGGCCC 1 cut(s) 361
ApeKI GCWGC 8 cut(s) 602, 633, 764, 767, 863, 902, 1061, 1154
AspLEI GCGC 2 cut(s) 146, 302
AspS9I GGNCC 5 cut(s) 256, 282, 357, 358, 397
AsuC2I CCSGG 1 cut(s) 254
AsuHPI GGTGA 3 cut(s) 103, 508, 974
AvaII GGWCC 2 cut(s) 256, 282
AxyI CCTNAGG 1 cut(s) 278
BaeGI GKGCMC 2 cut(s) 361, 406
BanII GRGCYC 3 cut(s) 108, 195, 361
BauI CACGAG 1 cut(s) 437
BbrPI CACGTG 1 cut(s) 480
BbsI GAAGAC 4 cut(s) 59, 379, 383, 425
Bbv12I GWGCWC 2 cut(s) 108, 841
BbvCI CCTCAGC 2 cut(s) 168, 760
BbvI GCAGC 8 cut(s) 589, 620, 776, 779, 850, 889, 1048, 1141
BccI CCATC 4 cut(s) 31, 214, 916, 993
BceAI ACGGC 1 cut(s) 384
BciT130I CCWGG 2 cut(s) 75, 196
BcnI CCSGG 1 cut(s) 254
BcoDI GTCTC 1 cut(s) 1070
BcuI ACTAGT 1 cut(s) 833
BfaI CTAG 1 cut(s) 834
BfmI CTRYAG 2 cut(s) 1065, 1155
BfoI RGCGCY 2 cut(s) 147, 303
Bme1390I CCNGG 3 cut(s) 75, 196, 254
Bme18I GGWCC 2 cut(s) 256, 282
BmgT120I GGNCC 5 cut(s) 256, 282, 357, 358, 397
BmiI GGNNCC 4 cut(s) 86, 257, 358, 359
BmrFI CCNGG 3 cut(s) 75, 196, 254
BmrI ACTGGG 1 cut(s) 871
BmsI GCATC 1 cut(s) 1060
BmuI ACTGGG 1 cut(s) 871
BoxI GACNNNNGTC 1 cut(s) 383
BpiI GAAGAC 4 cut(s) 59, 379, 383, 425
BpmI CTGGAG 1 cut(s) 334
Bpu10I CCTNAGC 2 cut(s) 168, 760
BpuEI CTTGAG 1 cut(s) 534
BpuMI CCSGG 1 cut(s) 254
Bsa29I ATCGAT 1 cut(s) 1198
BsaAI YACGTR 1 cut(s) 480
BsaBI GATNNNNATC 1 cut(s) 657
BsaHI GRCGYC 1 cut(s) 156
BsaJI CCNNGG 4 cut(s) 129, 194, 195, 409
BsaWI WCCGGW 1 cut(s) 258
BsaXI ACNNNNNCTCC 6 cut(s) 332, 362, 449, 479, 823, 853
Bsc4I CCNNNNNNNGG 4 cut(s) 279, 354, 366, 454
Bse118I RCCGGY 3 cut(s) 272, 296, 320
Bse1I ACTGG 5 cut(s) 314, 351, 634, 832, 877
Bse21I CCTNAGG 1 cut(s) 278
Bse3DI GCAATG 2 cut(s) 644, 965
Bse8I GATNNNNATC 1 cut(s) 657
BseBI CCWGG 2 cut(s) 75, 196
BseCI ATCGAT 1 cut(s) 1198
BseDI CCNNGG 4 cut(s) 129, 194, 195, 409
BseGI GGATG 5 cut(s) 375, 493, 658, 755, 985
BseJI GATNNNNATC 1 cut(s) 657
BseLI CCNNNNNNNGG 4 cut(s) 279, 354, 366, 454
BseMI GCAATG 2 cut(s) 644, 965
BseMII CTCAG 4 cut(s) 159, 676, 774, 1065
BseNI ACTGG 5 cut(s) 314, 351, 634, 832, 877
BseRI GAGGAG 3 cut(s) 116, 454, 788
BseSI GKGCMC 2 cut(s) 361, 406
BseXI GCAGC 8 cut(s) 589, 620, 776, 779, 850, 889, 1048, 1141
BsgI GTGCAG 1 cut(s) 758
Bsh1236I CGCG 1 cut(s) 411
Bsh1285I CGRYCG 1 cut(s) 273
BshFI GGCC 4 cut(s) 276, 359, 399, 414
BshVI ATCGAT 1 cut(s) 1198
BsiEI CGRYCG 1 cut(s) 273
BsiHKAI GWGCWC 2 cut(s) 108, 841
BsiSI CCGG 5 cut(s) 253, 259, 273, 297, 321
BslFI GGGAC 5 cut(s) 239, 269, 470, 539, 941
BslI CCNNNNNNNGG 4 cut(s) 279, 354, 366, 454
BsmAI GTCTC 1 cut(s) 1070
BsmFI GGGAC 5 cut(s) 239, 269, 470, 539, 941
BsnI GGCC 4 cut(s) 276, 359, 399, 414
Bsp120I GGGCCC 1 cut(s) 357
Bsp1286I GDGCHC 5 cut(s) 108, 195, 361, 406, 841
Bsp143I GATC 3 cut(s) 175, 814, 1132
BspACI CCGC 6 cut(s) 153, 409, 411, 445, 472, 1086
BspANI GGCC 4 cut(s) 276, 359, 399, 414
BspCNI CTCAG 4 cut(s) 160, 675, 773, 1066
BspDI ATCGAT 1 cut(s) 1198
BspFNI CGCG 1 cut(s) 411
BspLI GGNNCC 4 cut(s) 86, 257, 358, 359
BspMAI CTGCAG 1 cut(s) 1159
BspPI GGATC 1 cut(s) 1127
BspQI GCTCTTC 2 cut(s) 42, 791
BsrDI GCAATG 2 cut(s) 644, 965
BsrFI RCCGGY 3 cut(s) 272, 296, 320
BsrI ACTGG 5 cut(s) 314, 351, 634, 832, 877
BssAI RCCGGY 3 cut(s) 272, 296, 320
BssECI CCNNGG 4 cut(s) 129, 194, 195, 409
BssMI GATC 3 cut(s) 175, 814, 1132
BssNI GRCGYC 1 cut(s) 156
BssSI CACGAG 1 cut(s) 437
BssT1I CCWWGG 1 cut(s) 129
Bst2BI CACGAG 1 cut(s) 437
Bst2UI CCWGG 2 cut(s) 75, 196
Bst4CI ACNGT 3 cut(s) 344, 723, 849
Bst6I CTCTTC 2 cut(s) 42, 791
BstACI GRCGYC 1 cut(s) 156
BstAPI GCANNNNNTGC 2 cut(s) 781, 863
BstBAI YACGTR 1 cut(s) 480
BstC8I GCNNGC 5 cut(s) 304, 546, 638, 823, 1114
BstDEI CTNAG 5 cut(s) 168, 278, 662, 760, 1074
BstDSI CCRYGG 1 cut(s) 409
BstF5I GGATG 5 cut(s) 375, 493, 658, 755, 985
BstFNI CGCG 1 cut(s) 411
BstH2I RGCGCY 2 cut(s) 147, 303
BstHHI GCGC 2 cut(s) 146, 302
BstKTI GATC 3 cut(s) 178, 817, 1135
BstMAI GTCTC 1 cut(s) 1070
BstMBI GATC 3 cut(s) 175, 814, 1132
BstMCI CGRYCG 1 cut(s) 273
BstNI CCWGG 2 cut(s) 75, 196
BstPAI GACNNNNGTC 1 cut(s) 383
BstSCI CCNGG 3 cut(s) 73, 194, 252
BstSFI CTRYAG 2 cut(s) 1065, 1155
BstSLI GKGCMC 2 cut(s) 361, 406
BstUI CGCG 1 cut(s) 411
BstV1I GCAGC 8 cut(s) 589, 620, 776, 779, 850, 889, 1048, 1141
BstV2I GAAGAC 4 cut(s) 59, 379, 383, 425
BstX2I RGATCY 1 cut(s) 1132
BstYI RGATCY 1 cut(s) 1132
Bsu15I ATCGAT 1 cut(s) 1198
Bsu36I CCTNAGG 1 cut(s) 278
BsuRI GGCC 4 cut(s) 276, 359, 399, 414
BsuTUI ATCGAT 1 cut(s) 1198
BtgI CCRYGG 1 cut(s) 409
BtgZI GCGATG 1 cut(s) 148
BtsCI GGATG 5 cut(s) 375, 493, 658, 755, 985
BtsI GCAGTG 2 cut(s) 539, 770
BtsIMutI CAGTG 6 cut(s) 349, 358, 539, 770, 825, 854
Cac8I GCNNGC 5 cut(s) 304, 546, 638, 823, 1114
CaiI CAGNNNCTG 1 cut(s) 863
CfoI GCGC 2 cut(s) 146, 302
Cfr10I RCCGGY 3 cut(s) 272, 296, 320
Cfr13I GGNCC 5 cut(s) 256, 282, 357, 358, 397
Cfr42I CCGCGG 1 cut(s) 412
ClaI ATCGAT 1 cut(s) 1198
CseI GACGC 1 cut(s) 145
CsiI ACCWGGT 1 cut(s) 73
Csp6I GTAC 4 cut(s) 238, 265, 442, 831
CviAII CATG 3 cut(s) 433, 781, 1126
CviQI GTAC 4 cut(s) 238, 265, 442, 831
DdeI CTNAG 5 cut(s) 168, 278, 662, 760, 1074
DpnI GATC 3 cut(s) 177, 816, 1134
DpnII GATC 3 cut(s) 175, 814, 1132
EaeI YGGCCR 1 cut(s) 412
Eam1104I CTCTTC 2 cut(s) 42, 791
EarI CTCTTC 2 cut(s) 42, 791
Ecl136II GAGCTC 1 cut(s) 106
Eco130I CCWWGG 1 cut(s) 129
Eco24I GRGCYC 3 cut(s) 108, 195, 361
Eco32I GATATC 1 cut(s) 1177
Eco47I GGWCC 2 cut(s) 256, 282
Eco53kI GAGCTC 1 cut(s) 106
Eco57I CTGAAG 2 cut(s) 229, 956
Eco72I CACGTG 1 cut(s) 480
Eco81I CCTNAGG 1 cut(s) 278
EcoICRI GAGCTC 1 cut(s) 106
EcoO109I RGGNCCY 2 cut(s) 357, 358
EcoRII CCWGG 2 cut(s) 73, 194
EcoRV GATATC 1 cut(s) 1177
EcoT14I CCWWGG 1 cut(s) 129
EcoT38I GRGCYC 3 cut(s) 108, 195, 361
ErhI CCWWGG 1 cut(s) 129
FaeI CATG 3 cut(s) 436, 784, 1129
FaqI GGGAC 5 cut(s) 239, 269, 470, 539, 941
FatI CATG 3 cut(s) 432, 780, 1125
FauI CCCGC 1 cut(s) 416
FblI GTMKAC 2 cut(s) 135, 159
FokI GGATG 5 cut(s) 382, 500, 665, 742, 972
FriOI GRGCYC 3 cut(s) 108, 195, 361
FspBI CTAG 1 cut(s) 834
GlaI GCGC 2 cut(s) 145, 301
GsuI CTGGAG 1 cut(s) 334
HaeII RGCGCY 2 cut(s) 147, 303
HaeIII GGCC 4 cut(s) 276, 359, 399, 414
HapII CCGG 5 cut(s) 253, 259, 273, 297, 321
HgaI GACGC 1 cut(s) 145
HhaI GCGC 2 cut(s) 146, 302
Hin1I GRCGYC 1 cut(s) 156
Hin1II CATG 3 cut(s) 436, 784, 1129
Hin6I GCGC 2 cut(s) 144, 300
HinP1I GCGC 2 cut(s) 144, 300
HincII GTYRAC 1 cut(s) 160
HindII GTYRAC 1 cut(s) 160
HinfI GANTC 6 cut(s) 669, 931, 962, 1160, 1168, 1186
HpaII CCGG 5 cut(s) 253, 259, 273, 297, 321
HphI GGTGA 3 cut(s) 103, 508, 974
Hpy166II GTNNAC 3 cut(s) 136, 160, 340
Hpy188I TCNGA 4 cut(s) 665, 677, 1122, 1207
Hpy188III TCNNGA 3 cut(s) 689, 812, 1172
Hpy8I GTNNAC 3 cut(s) 136, 160, 340
Hpy99I CGWCG 2 cut(s) 161, 455
HpyAV CCTTC 2 cut(s) 371, 557
HpyCH4III ACNGT 3 cut(s) 344, 723, 849
HpyCH4IV ACGT 2 cut(s) 479, 954
HpyCH4V TGCA 8 cut(s) 436, 483, 544, 586, 775, 852, 998, 1157
HpyF3I CTNAG 5 cut(s) 168, 278, 662, 760, 1074
HpySE526I ACGT 2 cut(s) 479, 954
Hsp92I GRCGYC 1 cut(s) 156
Hsp92II CATG 3 cut(s) 436, 784, 1129
HspAI GCGC 2 cut(s) 144, 300
KspI CCGCGG 1 cut(s) 412
Kzo9I GATC 3 cut(s) 175, 814, 1132
LguI GCTCTTC 2 cut(s) 42, 791
LmnI GCTCC 3 cut(s) 103, 199, 844
Lsp1109I GCAGC 8 cut(s) 589, 620, 776, 779, 850, 889, 1048, 1141
LweI GCATC 1 cut(s) 1060
MabI ACCWGGT 1 cut(s) 73
MaeI CTAG 1 cut(s) 834
MaeII ACGT 2 cut(s) 479, 954
MaeIII GTNAC 1 cut(s) 792
MalI GATC 3 cut(s) 177, 816, 1134
MboI GATC 3 cut(s) 175, 814, 1132
MflI RGATCY 1 cut(s) 1132
MhlI GDGCHC 5 cut(s) 108, 195, 361, 406, 841
MluCI AATT 2 cut(s) 577, 684
MlyI GAGTC 2 cut(s) 663, 956
MseI TTAA 4 cut(s) 11, 897, 911, 1182
MspA1I CMGCKG 1 cut(s) 411
MspI CCGG 5 cut(s) 253, 259, 273, 297, 321
MspR9I CCNGG 3 cut(s) 75, 196, 254
MvaI CCWGG 2 cut(s) 75, 196
MvnI CGCG 1 cut(s) 411
NciI CCSGG 1 cut(s) 254
NdeII GATC 3 cut(s) 175, 814, 1132
NlaIII CATG 3 cut(s) 436, 784, 1129
NlaIV GGNNCC 4 cut(s) 86, 257, 358, 359
NmuCI GTSAC 1 cut(s) 792
PasI CCCWGGG 1 cut(s) 195
PciSI GCTCTTC 2 cut(s) 42, 791
PcsI WCGNNNNNNNCGW 1 cut(s) 171
PfeI GAWTC 4 cut(s) 931, 1160, 1168, 1186
PfoI TCCNGGA 1 cut(s) 252
PleI GAGTC 2 cut(s) 663, 956
PmaCI CACGTG 1 cut(s) 480
PmlI CACGTG 1 cut(s) 480
PpsI GAGTC 2 cut(s) 663, 956
Ppu21I YACGTR 1 cut(s) 480
PshAI GACNNNNGTC 1 cut(s) 383
Psp124BI GAGCTC 1 cut(s) 108
Psp6I CCWGG 2 cut(s) 73, 194
PspCI CACGTG 1 cut(s) 480
PspGI CCWGG 2 cut(s) 73, 194
PspN4I GGNNCC 4 cut(s) 86, 257, 358, 359
PspOMI GGGCCC 1 cut(s) 357
PspPI GGNCC 5 cut(s) 256, 282, 357, 358, 397
PstI CTGCAG 1 cut(s) 1159
PstNI CAGNNNCTG 1 cut(s) 863
PsuI RGATCY 1 cut(s) 1132
RsaI GTAC 4 cut(s) 239, 266, 443, 832
RsaNI GTAC 4 cut(s) 238, 265, 442, 831
SacI GAGCTC 1 cut(s) 108
SacII CCGCGG 1 cut(s) 412
SalI GTCGAC 1 cut(s) 158
SapI GCTCTTC 2 cut(s) 42, 791
SaqAI TTAA 4 cut(s) 11, 897, 911, 1182
Sau3AI GATC 3 cut(s) 175, 814, 1132
Sau96I GGNCC 5 cut(s) 256, 282, 357, 358, 397
SchI GAGTC 2 cut(s) 663, 956
ScrFI CCNGG 3 cut(s) 75, 196, 254
SduI GDGCHC 5 cut(s) 108, 195, 361, 406, 841
SexAI ACCWGGT 1 cut(s) 73
SfaNI GCATC 1 cut(s) 1060
SfcI CTRYAG 2 cut(s) 1065, 1155
Sfr303I CCGCGG 1 cut(s) 412
SgrAI CRCCGGYG 1 cut(s) 296
SgrBI CCGCGG 1 cut(s) 412
SinI GGWCC 2 cut(s) 256, 282
SmlI CTYRAG 1 cut(s) 549
SmoI CTYRAG 1 cut(s) 549
SpeI ACTAGT 1 cut(s) 833
Sse9I AATT 2 cut(s) 577, 684
SsiI CCGC 6 cut(s) 153, 409, 411, 445, 472, 1086
SspMI CTAG 1 cut(s) 834
SstI GAGCTC 1 cut(s) 108
StyD4I CCNGG 3 cut(s) 73, 194, 252
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 3 cut(s) 344, 723, 849
TaiI ACGT 2 cut(s) 482, 957
TaqI TCGA 5 cut(s) 159, 174, 450, 1008, 1198
TasI AATT 2 cut(s) 577, 684
TauI GCSGC 2 cut(s) 156, 414
TfiI GAWTC 4 cut(s) 931, 1160, 1168, 1186
Tru1I TTAA 4 cut(s) 11, 897, 911, 1182
Tru9I TTAA 4 cut(s) 11, 897, 911, 1182
TscAI CASTG 6 cut(s) 349, 358, 546, 777, 832, 854
TseFI GTSAC 1 cut(s) 792
TseI GCWGC 8 cut(s) 602, 633, 764, 767, 863, 902, 1061, 1154
Tsp45I GTSAC 1 cut(s) 792
TspDTI ATGAA 5 cut(s) 386, 504, 719, 744, 968
TspGWI ACGGA 1 cut(s) 1081
TspRI CASTG 6 cut(s) 349, 358, 546, 777, 832, 854
VpaK11BI GGWCC 2 cut(s) 256, 282
XcmI CCANNNNNNNNNTGG 1 cut(s) 1025
XmiI GTMKAC 2 cut(s) 135, 159
XspI CTAG 1 cut(s) 834
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.