Rh7AG300000

Protein CUP-SHAPED COTYLEDON

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0013567
Physical Location & Seq
Reverse (-)
32886398 .. 32889346
2949 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG300000.1

Sequence Viewer

Length: 1002 bp
ATGGGGGACAAAGAGTGGTACTTCTTCAGCCTCCGGGACCGGAAGTACCCGACCGGCCTTAGGACCAACCGAGCCACCGGCGCTGGCTACTGGAAAGCCACCGGCAAAGATAAAGAGGTTTACAGTGCCTCCAGTGGGGCCCTTCTTGGGATGAAGAAGACCCTCGTCTTCTACAAGGGCCGTGCCCCCCGCGGCCAGAAGACCAAGTGGGTCATGCACGAGTACCGCCTCGACGGGGACTTCTCCTCCTACCGCCACACGTGCAAGGATGAATGGGTGATATGCAGAATACATAACAAATCTGGGGACAAAAAAAACCCACTGCAAGCTCAAGGGCAAGGGGAAGGGCAACAGAGTAATTTCCTGCAACAAGTTGTGGAAGCTGCTTCTGCTTCTGCTTCTGCTTCTTCCAAAACTGGCTGCTTGCCTCCATTGCTGGAATGTCAATCTCAGACGACTCATCAGAACCCTAGTTTTCTGCAAAGCCCTAGTTTACTGCAGATTCAAGAAAACGACTTGATCAAAAGTCGTAATCAACTGTTTCAACAACCCTCATTATCATCATCATCTTCATCCTCAGCAGCAGCACTGCACTCCATGCTCTTCAAGTCACTCCTCTATTCACATCAAGATCAAGCTGGCACTGGTACTAGTGCTCCTAAACAGTGCAAGGCAGAGGCTGCTAATCTTTCCCAGTTCCAAACCCCACCTCTTAATGCTGCTAACTTCAACTACTTGATGGAAAAGAATCACTCTCATCATCAACCAAACGTCCCAGACTCATTGCTTCAGTTTCATCACCATCCCCGAAATGCACTCTCTTTCGATGACTTCCATTATTACAATGGTGTGTTGGGGCTTTCTACATCTGCTACTGCCGCTGTAGGTGCTGAGACTTCTTCCGCTTCCATTGGTGGTTTCAACAGGGCAGGCTTTCAGACCATGTTAGATCCTCTCATCAAAATCCCTGCTGCAGAATCTTGGAATCTGGATATCGCTTAA

Protein Analysis

333

Amino Acids

36.67

Weight (kDa)

9.06

Isoelectric Point (pI)

37.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 2 - 77 5.5e-26 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013567)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G76420
fragaria_vesca FvH4_5g25960 FvH4_5g25960 FvH4_5g25960
malus_domestica MD06G1004900.v1.1
prunus_persica Prupe.5G006200_v2.0.a1
pyrus_communis pycom06g00440 pycom16g26170
rosa_chinensis RchiOBHm_Chr7g0216521
rosa_laevigata RLG00000002564
rosa_multiflora Rmu_sc0009638.1_g000003 Rmu_sc0009638.1_g000004
rosa_roxburghii Rroxscaffold_3G00243140
rosa_rugosa Rorug07G0160700
rosa_samantha Rh7AG300000 Rh7BG291900 Rh7CG318600
rosa_wichuraiana Rw7G025510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 192
AciI CCGC 6 cut(s) 190, 192, 226, 253, 879, 903
AclWI GGATC 1 cut(s) 944
AcoI YGGCCR 1 cut(s) 193
AcuI CTGAAG 2 cut(s) 10, 773
AcvI CACGTG 1 cut(s) 261
AfaI GTAC 4 cut(s) 20, 47, 224, 649
AfiI CCNNNNNNNGG 4 cut(s) 60, 135, 147, 235
AflIII ACRYGT 1 cut(s) 258
AgsI TTSAA 5 cut(s) 506, 545, 607, 730, 922
AhlI ACTAGT 1 cut(s) 650
AluBI AGCT 3 cut(s) 329, 383, 638
AluI AGCT 3 cut(s) 329, 383, 638
Alw21I GWGCWC 1 cut(s) 658
Alw26I GTCTC 1 cut(s) 887
AlwI GGATC 1 cut(s) 944
AlwNI CAGNNNCTG 1 cut(s) 680
AoxI GGCC 4 cut(s) 55, 138, 178, 193
ApaI GGGCCC 1 cut(s) 142
ApeKI GCWGC 7 cut(s) 383, 420, 581, 584, 680, 719, 971
AspLEI GCGC 1 cut(s) 83
AspS9I GGNCC 5 cut(s) 37, 63, 138, 139, 178
AsuC2I CCSGG 1 cut(s) 35
AsuHPI GGTGA 2 cut(s) 289, 791
AvaII GGWCC 2 cut(s) 37, 63
AxyI CCTNAGG 1 cut(s) 59
BaeGI GKGCMC 2 cut(s) 142, 187
BanII GRGCYC 1 cut(s) 142
BauI CACGAG 1 cut(s) 218
BbrPI CACGTG 1 cut(s) 261
BbsI GAAGAC 3 cut(s) 160, 164, 206
Bbv12I GWGCWC 1 cut(s) 658
BbvCI CCTCAGC 1 cut(s) 577
BbvI GCAGC 7 cut(s) 370, 407, 593, 596, 667, 706, 958
BccI CCATC 2 cut(s) 733, 810
BceAI ACGGC 1 cut(s) 165
BclI TGATCA 1 cut(s) 519
BcnI CCSGG 1 cut(s) 35
BcoDI GTCTC 1 cut(s) 887
BcuI ACTAGT 1 cut(s) 650
BfaI CTAG 3 cut(s) 471, 489, 651
BfmI CTRYAG 3 cut(s) 497, 882, 972
BfoI RGCGCY 1 cut(s) 84
BisI GCNGC 9 cut(s) 193, 384, 421, 582, 585, 681, 720, 879, 972
BlsI GCNGC 9 cut(s) 194, 385, 422, 583, 586, 682, 721, 880, 973
Bme1390I CCNGG 1 cut(s) 35
Bme18I GGWCC 2 cut(s) 37, 63
BmgT120I GGNCC 5 cut(s) 37, 63, 138, 139, 178
BmiI GGNNCC 3 cut(s) 38, 139, 140
BmrFI CCNGG 1 cut(s) 35
BmrI ACTGGG 1 cut(s) 688
BmuI ACTGGG 1 cut(s) 688
BoxI GACNNNNGTC 1 cut(s) 164
BpiI GAAGAC 3 cut(s) 160, 164, 206
BpmI CTGGAG 1 cut(s) 115
Bpu10I CCTNAGC 1 cut(s) 577
BpuEI CTTGAG 1 cut(s) 315
BpuMI CCSGG 1 cut(s) 35
BsaAI YACGTR 1 cut(s) 261
BsaJI CCNNGG 1 cut(s) 190
BsaWI WCCGGW 1 cut(s) 39
BsaXI ACNNNNNCTCC 6 cut(s) 113, 143, 230, 260, 640, 670
Bsc4I CCNNNNNNNGG 4 cut(s) 60, 135, 147, 235
Bse118I RCCGGY 3 cut(s) 53, 77, 101
Bse1I ACTGG 5 cut(s) 95, 132, 421, 649, 694
Bse21I CCTNAGG 1 cut(s) 59
Bse3DI GCAATG 2 cut(s) 431, 782
BseDI CCNNGG 1 cut(s) 190
BseGI GGATG 4 cut(s) 156, 274, 572, 802
BseLI CCNNNNNNNGG 4 cut(s) 60, 135, 147, 235
BseMI GCAATG 2 cut(s) 431, 782
BseMII CTCAG 3 cut(s) 464, 591, 882
BseNI ACTGG 5 cut(s) 95, 132, 421, 649, 694
BseRI GAGGAG 2 cut(s) 235, 605
BseSI GKGCMC 2 cut(s) 142, 187
BseXI GCAGC 7 cut(s) 370, 407, 593, 596, 667, 706, 958
BsgI GTGCAG 1 cut(s) 575
Bsh1236I CGCG 1 cut(s) 192
Bsh1285I CGRYCG 1 cut(s) 54
BshFI GGCC 4 cut(s) 57, 140, 180, 195
BsiEI CGRYCG 1 cut(s) 54
BsiHKAI GWGCWC 1 cut(s) 658
BsiSI CCGG 5 cut(s) 34, 40, 54, 78, 102
BslFI GGGAC 5 cut(s) 20, 50, 251, 320, 758
BslI CCNNNNNNNGG 4 cut(s) 60, 135, 147, 235
BsmAI GTCTC 1 cut(s) 887
BsmFI GGGAC 5 cut(s) 20, 50, 251, 320, 758
BsnI GGCC 4 cut(s) 57, 140, 180, 195
Bsp120I GGGCCC 1 cut(s) 138
Bsp1286I GDGCHC 3 cut(s) 142, 187, 658
Bsp143I GATC 3 cut(s) 519, 631, 949
BspACI CCGC 6 cut(s) 190, 192, 226, 253, 879, 903
BspANI GGCC 4 cut(s) 57, 140, 180, 195
BspCNI CTCAG 3 cut(s) 463, 590, 883
BspFNI CGCG 1 cut(s) 192
BspLI GGNNCC 3 cut(s) 38, 139, 140
BspMAI CTGCAG 2 cut(s) 501, 976
BspPI GGATC 1 cut(s) 944
BspQI GCTCTTC 1 cut(s) 608
BsrDI GCAATG 2 cut(s) 431, 782
BsrFI RCCGGY 3 cut(s) 53, 77, 101
BsrI ACTGG 5 cut(s) 95, 132, 421, 649, 694
BssAI RCCGGY 3 cut(s) 53, 77, 101
BssECI CCNNGG 1 cut(s) 190
BssMI GATC 3 cut(s) 519, 631, 949
BssSI CACGAG 1 cut(s) 218
Bst2BI CACGAG 1 cut(s) 218
Bst4CI ACNGT 3 cut(s) 125, 540, 666
Bst6I CTCTTC 1 cut(s) 608
BstAPI GCANNNNNTGC 2 cut(s) 598, 680
BstBAI YACGTR 1 cut(s) 261
BstC8I GCNNGC 5 cut(s) 85, 327, 425, 640, 931
BstDEI CTNAG 4 cut(s) 59, 450, 577, 891
BstDSI CCRYGG 1 cut(s) 190
BstF5I GGATG 4 cut(s) 156, 274, 572, 802
BstFNI CGCG 1 cut(s) 192
BstH2I RGCGCY 1 cut(s) 84
BstHHI GCGC 1 cut(s) 83
BstKTI GATC 3 cut(s) 522, 634, 952
BstMAI GTCTC 1 cut(s) 887
BstMBI GATC 3 cut(s) 519, 631, 949
BstMCI CGRYCG 1 cut(s) 54
BstMWI GCNNNNNNNGC 8 cut(s) 80, 261, 389, 433, 598, 680, 878, 887
BstPAI GACNNNNGTC 1 cut(s) 164
BstSCI CCNGG 1 cut(s) 33
BstSFI CTRYAG 3 cut(s) 497, 882, 972
BstSLI GKGCMC 2 cut(s) 142, 187
BstUI CGCG 1 cut(s) 192
BstV1I GCAGC 7 cut(s) 370, 407, 593, 596, 667, 706, 958
BstV2I GAAGAC 3 cut(s) 160, 164, 206
BstX2I RGATCY 1 cut(s) 949
BstYI RGATCY 1 cut(s) 949
Bsu36I CCTNAGG 1 cut(s) 59
BsuRI GGCC 4 cut(s) 57, 140, 180, 195
BtgI CCRYGG 1 cut(s) 190
BtsCI GGATG 4 cut(s) 156, 274, 572, 802
BtsI GCAGTG 2 cut(s) 320, 587
BtsIMutI CAGTG 6 cut(s) 130, 139, 320, 587, 642, 671
Cac8I GCNNGC 5 cut(s) 85, 327, 425, 640, 931
CaiI CAGNNNCTG 1 cut(s) 680
CfoI GCGC 1 cut(s) 83
Cfr10I RCCGGY 3 cut(s) 53, 77, 101
Cfr13I GGNCC 5 cut(s) 37, 63, 138, 139, 178
Cfr42I CCGCGG 1 cut(s) 193
Csp6I GTAC 4 cut(s) 19, 46, 223, 648
CviAII CATG 3 cut(s) 214, 598, 943
CviQI GTAC 4 cut(s) 19, 46, 223, 648
DdeI CTNAG 4 cut(s) 59, 450, 577, 891
DpnI GATC 3 cut(s) 521, 633, 951
DpnII GATC 3 cut(s) 519, 631, 949
EaeI YGGCCR 1 cut(s) 193
Eam1104I CTCTTC 1 cut(s) 608
EarI CTCTTC 1 cut(s) 608
Eco24I GRGCYC 1 cut(s) 142
Eco32I GATATC 1 cut(s) 994
Eco47I GGWCC 2 cut(s) 37, 63
Eco57I CTGAAG 2 cut(s) 10, 773
Eco72I CACGTG 1 cut(s) 261
Eco81I CCTNAGG 1 cut(s) 59
EcoO109I RGGNCCY 2 cut(s) 138, 139
EcoRV GATATC 1 cut(s) 994
EcoT38I GRGCYC 1 cut(s) 142
FaeI CATG 3 cut(s) 217, 601, 946
FaiI YATR 5 cut(s) 215, 283, 294, 599, 944
FaqI GGGAC 5 cut(s) 20, 50, 251, 320, 758
FatI CATG 3 cut(s) 213, 597, 942
FauI CCCGC 1 cut(s) 197
FbaI TGATCA 1 cut(s) 519
Fnu4HI GCNGC 9 cut(s) 193, 384, 421, 582, 585, 681, 720, 879, 972
FokI GGATG 4 cut(s) 163, 281, 559, 789
FriOI GRGCYC 1 cut(s) 142
Fsp4HI GCNGC 9 cut(s) 193, 384, 421, 582, 585, 681, 720, 879, 972
FspBI CTAG 3 cut(s) 471, 489, 651
GlaI GCGC 1 cut(s) 82
GluI GCNGC 9 cut(s) 193, 384, 421, 582, 585, 681, 720, 879, 972
GsuI CTGGAG 1 cut(s) 115
HaeII RGCGCY 1 cut(s) 84
HaeIII GGCC 4 cut(s) 57, 140, 180, 195
HapII CCGG 5 cut(s) 34, 40, 54, 78, 102
HhaI GCGC 1 cut(s) 83
Hin1II CATG 3 cut(s) 217, 601, 946
Hin6I GCGC 1 cut(s) 81
HinP1I GCGC 1 cut(s) 81
HinfI GANTC 6 cut(s) 457, 502, 748, 779, 977, 985
HpaII CCGG 5 cut(s) 34, 40, 54, 78, 102
HphI GGTGA 2 cut(s) 289, 791
Hpy166II GTNNAC 2 cut(s) 121, 494
Hpy188I TCNGA 3 cut(s) 453, 465, 939
Hpy188III TCNNGA 3 cut(s) 506, 629, 989
Hpy8I GTNNAC 2 cut(s) 121, 494
Hpy99I CGWCG 1 cut(s) 236
HpyAV CCTTC 2 cut(s) 152, 338
HpyCH4III ACNGT 3 cut(s) 125, 540, 666
HpyCH4IV ACGT 2 cut(s) 260, 771
HpyF10VI GCNNNNNNNGC 8 cut(s) 80, 261, 389, 433, 598, 680, 878, 887
HpyF3I CTNAG 4 cut(s) 59, 450, 577, 891
HpySE526I ACGT 2 cut(s) 260, 771
Hsp92II CATG 3 cut(s) 217, 601, 946
HspAI GCGC 1 cut(s) 81
Ksp22I TGATCA 1 cut(s) 519
KspI CCGCGG 1 cut(s) 193
Kzo9I GATC 3 cut(s) 519, 631, 949
LguI GCTCTTC 1 cut(s) 608
LmnI GCTCC 1 cut(s) 661
Lsp1109I GCAGC 7 cut(s) 370, 407, 593, 596, 667, 706, 958
MaeI CTAG 3 cut(s) 471, 489, 651
MaeII ACGT 2 cut(s) 260, 771
MaeIII GTNAC 1 cut(s) 609
MalI GATC 3 cut(s) 521, 633, 951
MboI GATC 3 cut(s) 519, 631, 949
MboII GAAGA 9 cut(s) 16, 160, 166, 169, 211, 399, 561, 595, 891
MflI RGATCY 1 cut(s) 949
MhlI GDGCHC 3 cut(s) 142, 187, 658
MluCI AATT 1 cut(s) 358
MlyI GAGTC 2 cut(s) 451, 773
MseI TTAA 2 cut(s) 714, 1000
MspA1I CMGCKG 2 cut(s) 192, 881
MspI CCGG 5 cut(s) 34, 40, 54, 78, 102
MspR9I CCNGG 1 cut(s) 35
MvnI CGCG 1 cut(s) 192
MwoI GCNNNNNNNGC 8 cut(s) 80, 261, 389, 433, 598, 680, 878, 887
NciI CCSGG 1 cut(s) 35
NdeII GATC 3 cut(s) 519, 631, 949
NlaIII CATG 3 cut(s) 217, 601, 946
NlaIV GGNNCC 3 cut(s) 38, 139, 140
NmuCI GTSAC 1 cut(s) 609
PciSI GCTCTTC 1 cut(s) 608
PfeI GAWTC 4 cut(s) 502, 748, 977, 985
PfoI TCCNGGA 1 cut(s) 33
PkrI GCNGC 9 cut(s) 194, 385, 422, 583, 586, 682, 721, 880, 973
PleI GAGTC 2 cut(s) 451, 773
PmaCI CACGTG 1 cut(s) 261
PmlI CACGTG 1 cut(s) 261
PpsI GAGTC 2 cut(s) 451, 773
Ppu21I YACGTR 1 cut(s) 261
PshAI GACNNNNGTC 1 cut(s) 164
PspCI CACGTG 1 cut(s) 261
PspN4I GGNNCC 3 cut(s) 38, 139, 140
PspOMI GGGCCC 1 cut(s) 138
PspPI GGNCC 5 cut(s) 37, 63, 138, 139, 178
PstI CTGCAG 2 cut(s) 501, 976
PstNI CAGNNNCTG 1 cut(s) 680
PsuI RGATCY 1 cut(s) 949
RsaI GTAC 4 cut(s) 20, 47, 224, 649
RsaNI GTAC 4 cut(s) 19, 46, 223, 648
SacII CCGCGG 1 cut(s) 193
SapI GCTCTTC 1 cut(s) 608
SaqAI TTAA 2 cut(s) 714, 1000
SatI GCNGC 9 cut(s) 193, 384, 421, 582, 585, 681, 720, 879, 972
Sau3AI GATC 3 cut(s) 519, 631, 949
Sau96I GGNCC 5 cut(s) 37, 63, 138, 139, 178
SchI GAGTC 2 cut(s) 451, 773
ScrFI CCNGG 1 cut(s) 35
SduI GDGCHC 3 cut(s) 142, 187, 658
SetI ASST 8 cut(s) 120, 263, 331, 385, 640, 712, 774, 889
SfcI CTRYAG 3 cut(s) 497, 882, 972
Sfr303I CCGCGG 1 cut(s) 193
SgrAI CRCCGGYG 1 cut(s) 77
SgrBI CCGCGG 1 cut(s) 193
SinI GGWCC 2 cut(s) 37, 63
SmlI CTYRAG 1 cut(s) 330
SmoI CTYRAG 1 cut(s) 330
SpeI ACTAGT 1 cut(s) 650
Sse9I AATT 1 cut(s) 358
SsiI CCGC 6 cut(s) 190, 192, 226, 253, 879, 903
SspMI CTAG 3 cut(s) 471, 489, 651
StyD4I CCNGG 1 cut(s) 33
TaaI ACNGT 3 cut(s) 125, 540, 666
TaiI ACGT 2 cut(s) 263, 774
TaqI TCGA 2 cut(s) 231, 825
TasI AATT 1 cut(s) 358
TauI GCSGC 2 cut(s) 195, 881
TfiI GAWTC 4 cut(s) 502, 748, 977, 985
Tru1I TTAA 2 cut(s) 714, 1000
Tru9I TTAA 2 cut(s) 714, 1000
TscAI CASTG 6 cut(s) 130, 139, 327, 594, 649, 671
TseFI GTSAC 1 cut(s) 609
TseI GCWGC 7 cut(s) 383, 420, 581, 584, 680, 719, 971
Tsp45I GTSAC 1 cut(s) 609
TspDTI ATGAA 4 cut(s) 167, 285, 561, 785
TspRI CASTG 6 cut(s) 130, 139, 327, 594, 649, 671
VpaK11BI GGWCC 2 cut(s) 37, 63
XcmI CCANNNNNNNNNTGG 1 cut(s) 842
XspI CTAG 3 cut(s) 471, 489, 651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.