RchiOBHm_Chr7g0216521

Protein CUP-SHAPED COTYLEDON

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0013567
Physical Location & Seq
Reverse (-)
34110077 .. 34113050
2974 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19374

Sequence Viewer

Length: 1215 bp
ATGTTGGGTTTAATGGAAGAAGTTATGAGTGAGCTGATGGGTAGGGAAGAGCTGAACGAACAAGGTCTTCCACCTGGTTTCAGGTTCCACCCAACTGATGAGGAGCTCATCACCTTTTACCTCGCTTCCAAGGTCTACAATGGCGCTTTCTGCGGCGTCGACATCGCTGAGGTCGATCTCAACAGATGTGAGCCCTGGGAGCTACCAGATGTGGCGAAGATGGGGGACAAAGAGTGGTACTTCTTCAGCCTCCGGGACCGGAAGTACCCGACCGGCCTTAGGACCAACCGAGCCACCGGCGCTGGCTACTGGAAAGCCACCGGCAAAGATAAAGAGGTTTACAGTGCCTCCAGTGGGGCCCTTCTTGGGATGAAGAAGACCCTCGTCTTCTACAAGGGCCGTGCCCCCCGCGGCCAGAAGACCAAGTGGGTCATGCACGAGTACCGCCTCGACGGGGACTTCTCCTCCTACCGCCACACGTGCAAGGATGAATGGGTGATATGCAGAATACATAACAAATCTGGGGACAAAAAAAACCCACTGCAAGCTCAAGGGCAAGGGGAAGGGCAACAGAGTAATTTCCTGCAACAAGTTGTGGAAGCTGCTTCTGCTTCTGCTTCTTCCAAAACTGGCTGCTTGCCTCCATTGCTGGAATGTCAATCTCAGACGACTCATCAGAACCATAGTTTTCTGCAAAGCCCTAGTTTACTGCAGATTCAAGAAAACGACTTGATGAAAAGTCGTAATCAACTGTTTCAACAACCCTCATTATCATCATCATCTTCATCCTCAGCAGCAGCACTGCACTCCATGCTCTTCAAGTCACTCCTCTATTCACATCAAGATCAAGCTGGCACTGGTAGTAGTGCTCCTAAACAGTGCAAGGCAGAGGCTGCTAATCTTTCCCAGTTCCAAACCCCACCCCTTAATGCTGCTAACTTCAACTACTTGATGGAAAAGAATCACTCTCATCATCAACCAAACGTCCCAGACTCATTGCTTCAGTTTCATCACCATCCCCGAAATGCACTCTCTTTCGATGACTTCCATTATTACAATGGTGTGTTGGGGCTTTCTACATCTGCTACTGCTGCTGTAGATGCTGAGACTTCTTCCGCTTCCATTGGTGGTTTCAACAGGGCAGGCTTTCAGACCATGTTAGATCCTCTCATCAAACTCCCTGCTGCAGAATCTTGGAATCTGGATATCGCTTAA

Protein Analysis

404

Amino Acids

44.89

Weight (kDa)

6.43

Isoelectric Point (pI)

39.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 24 - 150 8.7e-47 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013567)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G76420
fragaria_vesca FvH4_5g25960 FvH4_5g25960 FvH4_5g25960
malus_domestica MD06G1004900.v1.1
prunus_persica Prupe.5G006200_v2.0.a1
pyrus_communis pycom06g00440 pycom16g26170
rosa_chinensis RchiOBHm_Chr7g0216521
rosa_laevigata RLG00000002564
rosa_multiflora Rmu_sc0009638.1_g000003 Rmu_sc0009638.1_g000004
rosa_roxburghii Rroxscaffold_3G00243140
rosa_rugosa Rorug07G0160700
rosa_samantha Rh7AG300000 Rh7BG291900 Rh7CG318600
rosa_wichuraiana Rw7G025510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 135, 159
AccII CGCG 1 cut(s) 411
AciI CCGC 6 cut(s) 153, 409, 411, 445, 472, 1116
AclWI GGATC 1 cut(s) 1157
AcoI YGGCCR 1 cut(s) 412
AcuI CTGAAG 2 cut(s) 229, 986
AcvI CACGTG 1 cut(s) 480
AcyI GRCGYC 1 cut(s) 156
AfaI GTAC 3 cut(s) 239, 266, 443
AfiI CCNNNNNNNGG 4 cut(s) 279, 354, 366, 454
AflIII ACRYGT 1 cut(s) 477
AgsI TTSAA 5 cut(s) 719, 758, 820, 943, 1135
AjnI CCWGG 2 cut(s) 73, 194
AluBI AGCT 7 cut(s) 34, 52, 106, 202, 548, 602, 851
AluI AGCT 7 cut(s) 34, 52, 106, 202, 548, 602, 851
Alw21I GWGCWC 2 cut(s) 108, 871
Alw26I GTCTC 1 cut(s) 1100
AlwI GGATC 1 cut(s) 1157
AlwNI CAGNNNCTG 1 cut(s) 893
AoxI GGCC 4 cut(s) 274, 357, 397, 412
ApaI GGGCCC 1 cut(s) 361
ApeKI GCWGC 8 cut(s) 602, 633, 794, 797, 893, 932, 1091, 1184
AspLEI GCGC 2 cut(s) 146, 302
AspS9I GGNCC 5 cut(s) 256, 282, 357, 358, 397
AsuC2I CCSGG 1 cut(s) 254
AsuHPI GGTGA 3 cut(s) 103, 508, 1004
AvaII GGWCC 2 cut(s) 256, 282
AxyI CCTNAGG 1 cut(s) 278
BaeGI GKGCMC 2 cut(s) 361, 406
BanII GRGCYC 3 cut(s) 108, 195, 361
BauI CACGAG 1 cut(s) 437
BbrPI CACGTG 1 cut(s) 480
BbsI GAAGAC 4 cut(s) 59, 379, 383, 425
Bbv12I GWGCWC 2 cut(s) 108, 871
BbvCI CCTCAGC 2 cut(s) 168, 790
BbvI GCAGC 8 cut(s) 589, 620, 806, 809, 880, 919, 1078, 1171
BccI CCATC 4 cut(s) 31, 214, 946, 1023
BceAI ACGGC 1 cut(s) 384
BciT130I CCWGG 2 cut(s) 75, 196
BcnI CCSGG 1 cut(s) 254
BcoDI GTCTC 1 cut(s) 1100
BfaI CTAG 1 cut(s) 702
BfmI CTRYAG 3 cut(s) 710, 1095, 1185
BfoI RGCGCY 2 cut(s) 147, 303
Bme1390I CCNGG 3 cut(s) 75, 196, 254
Bme18I GGWCC 2 cut(s) 256, 282
BmgT120I GGNCC 5 cut(s) 256, 282, 357, 358, 397
BmiI GGNNCC 4 cut(s) 86, 257, 358, 359
BmrFI CCNGG 3 cut(s) 75, 196, 254
BmrI ACTGGG 1 cut(s) 901
BmsI GCATC 1 cut(s) 1090
BmuI ACTGGG 1 cut(s) 901
BoxI GACNNNNGTC 1 cut(s) 383
BpiI GAAGAC 4 cut(s) 59, 379, 383, 425
BpmI CTGGAG 1 cut(s) 334
Bpu10I CCTNAGC 2 cut(s) 168, 790
BpuEI CTTGAG 1 cut(s) 534
BpuMI CCSGG 1 cut(s) 254
BsaAI YACGTR 1 cut(s) 480
BsaHI GRCGYC 1 cut(s) 156
BsaJI CCNNGG 4 cut(s) 129, 194, 195, 409
BsaWI WCCGGW 1 cut(s) 258
BsaXI ACNNNNNCTCC 4 cut(s) 332, 362, 449, 479
Bsc4I CCNNNNNNNGG 4 cut(s) 279, 354, 366, 454
Bse118I RCCGGY 3 cut(s) 272, 296, 320
Bse1I ACTGG 5 cut(s) 314, 351, 634, 862, 907
Bse21I CCTNAGG 1 cut(s) 278
Bse3DI GCAATG 2 cut(s) 644, 995
BseBI CCWGG 2 cut(s) 75, 196
BseDI CCNNGG 4 cut(s) 129, 194, 195, 409
BseGI GGATG 4 cut(s) 375, 493, 785, 1015
BseLI CCNNNNNNNGG 4 cut(s) 279, 354, 366, 454
BseMI GCAATG 2 cut(s) 644, 995
BseMII CTCAG 4 cut(s) 159, 677, 804, 1095
BseNI ACTGG 5 cut(s) 314, 351, 634, 862, 907
BseRI GAGGAG 3 cut(s) 116, 454, 818
BseSI GKGCMC 2 cut(s) 361, 406
BseXI GCAGC 8 cut(s) 589, 620, 806, 809, 880, 919, 1078, 1171
BsgI GTGCAG 1 cut(s) 788
Bsh1236I CGCG 1 cut(s) 411
Bsh1285I CGRYCG 1 cut(s) 273
BshFI GGCC 4 cut(s) 276, 359, 399, 414
BsiEI CGRYCG 1 cut(s) 273
BsiHKAI GWGCWC 2 cut(s) 108, 871
BsiSI CCGG 5 cut(s) 253, 259, 273, 297, 321
BslFI GGGAC 5 cut(s) 239, 269, 470, 539, 971
BslI CCNNNNNNNGG 4 cut(s) 279, 354, 366, 454
BsmAI GTCTC 1 cut(s) 1100
BsmFI GGGAC 5 cut(s) 239, 269, 470, 539, 971
BsnI GGCC 4 cut(s) 276, 359, 399, 414
Bsp120I GGGCCC 1 cut(s) 357
Bsp1286I GDGCHC 5 cut(s) 108, 195, 361, 406, 871
Bsp143I GATC 3 cut(s) 175, 844, 1162
BspACI CCGC 6 cut(s) 153, 409, 411, 445, 472, 1116
BspANI GGCC 4 cut(s) 276, 359, 399, 414
BspCNI CTCAG 4 cut(s) 160, 676, 803, 1096
BspFNI CGCG 1 cut(s) 411
BspLI GGNNCC 4 cut(s) 86, 257, 358, 359
BspMAI CTGCAG 2 cut(s) 714, 1189
BspPI GGATC 1 cut(s) 1157
BspQI GCTCTTC 2 cut(s) 42, 821
BsrDI GCAATG 2 cut(s) 644, 995
BsrFI RCCGGY 3 cut(s) 272, 296, 320
BsrI ACTGG 5 cut(s) 314, 351, 634, 862, 907
BssAI RCCGGY 3 cut(s) 272, 296, 320
BssECI CCNNGG 4 cut(s) 129, 194, 195, 409
BssMI GATC 3 cut(s) 175, 844, 1162
BssNI GRCGYC 1 cut(s) 156
BssSI CACGAG 1 cut(s) 437
BssT1I CCWWGG 1 cut(s) 129
Bst2BI CACGAG 1 cut(s) 437
Bst2UI CCWGG 2 cut(s) 75, 196
Bst4CI ACNGT 3 cut(s) 344, 753, 879
Bst6I CTCTTC 2 cut(s) 42, 821
BstACI GRCGYC 1 cut(s) 156
BstAPI GCANNNNNTGC 2 cut(s) 811, 893
BstBAI YACGTR 1 cut(s) 480
BstC8I GCNNGC 5 cut(s) 304, 546, 638, 853, 1144
BstDEI CTNAG 5 cut(s) 168, 278, 663, 790, 1104
BstDSI CCRYGG 1 cut(s) 409
BstF5I GGATG 4 cut(s) 375, 493, 785, 1015
BstFNI CGCG 1 cut(s) 411
BstH2I RGCGCY 2 cut(s) 147, 303
BstHHI GCGC 2 cut(s) 146, 302
BstKTI GATC 3 cut(s) 178, 847, 1165
BstMAI GTCTC 1 cut(s) 1100
BstMBI GATC 3 cut(s) 175, 844, 1162
BstMCI CGRYCG 1 cut(s) 273
BstNI CCWGG 2 cut(s) 75, 196
BstPAI GACNNNNGTC 1 cut(s) 383
BstSCI CCNGG 3 cut(s) 73, 194, 252
BstSFI CTRYAG 3 cut(s) 710, 1095, 1185
BstSLI GKGCMC 2 cut(s) 361, 406
BstUI CGCG 1 cut(s) 411
BstV1I GCAGC 8 cut(s) 589, 620, 806, 809, 880, 919, 1078, 1171
BstV2I GAAGAC 4 cut(s) 59, 379, 383, 425
BstX2I RGATCY 1 cut(s) 1162
BstYI RGATCY 1 cut(s) 1162
Bsu36I CCTNAGG 1 cut(s) 278
BsuRI GGCC 4 cut(s) 276, 359, 399, 414
BtgI CCRYGG 1 cut(s) 409
BtgZI GCGATG 1 cut(s) 148
BtsCI GGATG 4 cut(s) 375, 493, 785, 1015
BtsI GCAGTG 2 cut(s) 539, 800
BtsIMutI CAGTG 6 cut(s) 349, 358, 539, 800, 855, 884
Cac8I GCNNGC 5 cut(s) 304, 546, 638, 853, 1144
CaiI CAGNNNCTG 1 cut(s) 893
CfoI GCGC 2 cut(s) 146, 302
Cfr10I RCCGGY 3 cut(s) 272, 296, 320
Cfr13I GGNCC 5 cut(s) 256, 282, 357, 358, 397
Cfr42I CCGCGG 1 cut(s) 412
CseI GACGC 1 cut(s) 145
CsiI ACCWGGT 1 cut(s) 73
Csp6I GTAC 3 cut(s) 238, 265, 442
CviAII CATG 3 cut(s) 433, 811, 1156
CviQI GTAC 3 cut(s) 238, 265, 442
DdeI CTNAG 5 cut(s) 168, 278, 663, 790, 1104
DpnI GATC 3 cut(s) 177, 846, 1164
DpnII GATC 3 cut(s) 175, 844, 1162
EaeI YGGCCR 1 cut(s) 412
Eam1104I CTCTTC 2 cut(s) 42, 821
EarI CTCTTC 2 cut(s) 42, 821
Ecl136II GAGCTC 1 cut(s) 106
Eco130I CCWWGG 1 cut(s) 129
Eco24I GRGCYC 3 cut(s) 108, 195, 361
Eco32I GATATC 1 cut(s) 1207
Eco47I GGWCC 2 cut(s) 256, 282
Eco53kI GAGCTC 1 cut(s) 106
Eco57I CTGAAG 2 cut(s) 229, 986
Eco72I CACGTG 1 cut(s) 480
Eco81I CCTNAGG 1 cut(s) 278
EcoICRI GAGCTC 1 cut(s) 106
EcoO109I RGGNCCY 2 cut(s) 357, 358
EcoRII CCWGG 2 cut(s) 73, 194
EcoRV GATATC 1 cut(s) 1207
EcoT14I CCWWGG 1 cut(s) 129
EcoT38I GRGCYC 3 cut(s) 108, 195, 361
ErhI CCWWGG 1 cut(s) 129
FaeI CATG 3 cut(s) 436, 814, 1159
FaiI YATR 7 cut(s) 26, 434, 502, 513, 684, 812, 1157
FaqI GGGAC 5 cut(s) 239, 269, 470, 539, 971
FatI CATG 3 cut(s) 432, 810, 1155
FauI CCCGC 1 cut(s) 416
FblI GTMKAC 2 cut(s) 135, 159
FokI GGATG 4 cut(s) 382, 500, 772, 1002
FriOI GRGCYC 3 cut(s) 108, 195, 361
FspBI CTAG 1 cut(s) 702
GlaI GCGC 2 cut(s) 145, 301
GsuI CTGGAG 1 cut(s) 334
HaeII RGCGCY 2 cut(s) 147, 303
HaeIII GGCC 4 cut(s) 276, 359, 399, 414
HapII CCGG 5 cut(s) 253, 259, 273, 297, 321
HgaI GACGC 1 cut(s) 145
HhaI GCGC 2 cut(s) 146, 302
Hin1I GRCGYC 1 cut(s) 156
Hin1II CATG 3 cut(s) 436, 814, 1159
Hin6I GCGC 2 cut(s) 144, 300
HinP1I GCGC 2 cut(s) 144, 300
HincII GTYRAC 1 cut(s) 160
HindII GTYRAC 1 cut(s) 160
HinfI GANTC 6 cut(s) 670, 715, 961, 992, 1190, 1198
HpaII CCGG 5 cut(s) 253, 259, 273, 297, 321
HphI GGTGA 3 cut(s) 103, 508, 1004
Hpy166II GTNNAC 4 cut(s) 136, 160, 340, 707
Hpy188I TCNGA 3 cut(s) 666, 678, 1152
Hpy188III TCNNGA 3 cut(s) 719, 842, 1202
Hpy8I GTNNAC 4 cut(s) 136, 160, 340, 707
Hpy99I CGWCG 2 cut(s) 161, 455
HpyAV CCTTC 2 cut(s) 371, 557
HpyCH4III ACNGT 3 cut(s) 344, 753, 879
HpyCH4IV ACGT 2 cut(s) 479, 984
HpyF3I CTNAG 5 cut(s) 168, 278, 663, 790, 1104
HpySE526I ACGT 2 cut(s) 479, 984
Hsp92I GRCGYC 1 cut(s) 156
Hsp92II CATG 3 cut(s) 436, 814, 1159
HspAI GCGC 2 cut(s) 144, 300
KspI CCGCGG 1 cut(s) 412
Kzo9I GATC 3 cut(s) 175, 844, 1162
LguI GCTCTTC 2 cut(s) 42, 821
LmnI GCTCC 3 cut(s) 103, 199, 874
Lsp1109I GCAGC 8 cut(s) 589, 620, 806, 809, 880, 919, 1078, 1171
LweI GCATC 1 cut(s) 1090
MabI ACCWGGT 1 cut(s) 73
MaeI CTAG 1 cut(s) 702
MaeII ACGT 2 cut(s) 479, 984
MaeIII GTNAC 1 cut(s) 822
MalI GATC 3 cut(s) 177, 846, 1164
MboI GATC 3 cut(s) 175, 844, 1162
MflI RGATCY 1 cut(s) 1162
MhlI GDGCHC 5 cut(s) 108, 195, 361, 406, 871
MluCI AATT 1 cut(s) 577
MlyI GAGTC 2 cut(s) 664, 986
MseI TTAA 3 cut(s) 11, 927, 1213
MspA1I CMGCKG 1 cut(s) 411
MspI CCGG 5 cut(s) 253, 259, 273, 297, 321
MspR9I CCNGG 3 cut(s) 75, 196, 254
MvaI CCWGG 2 cut(s) 75, 196
MvnI CGCG 1 cut(s) 411
NciI CCSGG 1 cut(s) 254
NdeII GATC 3 cut(s) 175, 844, 1162
NlaIII CATG 3 cut(s) 436, 814, 1159
NlaIV GGNNCC 4 cut(s) 86, 257, 358, 359
NmuCI GTSAC 1 cut(s) 822
PasI CCCWGGG 1 cut(s) 195
PciSI GCTCTTC 2 cut(s) 42, 821
PcsI WCGNNNNNNNCGW 1 cut(s) 171
PfeI GAWTC 4 cut(s) 715, 961, 1190, 1198
PfoI TCCNGGA 1 cut(s) 252
PleI GAGTC 2 cut(s) 664, 986
PmaCI CACGTG 1 cut(s) 480
PmlI CACGTG 1 cut(s) 480
PpsI GAGTC 2 cut(s) 664, 986
Ppu21I YACGTR 1 cut(s) 480
PshAI GACNNNNGTC 1 cut(s) 383
Psp124BI GAGCTC 1 cut(s) 108
Psp6I CCWGG 2 cut(s) 73, 194
PspCI CACGTG 1 cut(s) 480
PspGI CCWGG 2 cut(s) 73, 194
PspN4I GGNNCC 4 cut(s) 86, 257, 358, 359
PspOMI GGGCCC 1 cut(s) 357
PspPI GGNCC 5 cut(s) 256, 282, 357, 358, 397
PstI CTGCAG 2 cut(s) 714, 1189
PstNI CAGNNNCTG 1 cut(s) 893
PsuI RGATCY 1 cut(s) 1162
RsaI GTAC 3 cut(s) 239, 266, 443
RsaNI GTAC 3 cut(s) 238, 265, 442
SacI GAGCTC 1 cut(s) 108
SacII CCGCGG 1 cut(s) 412
SalI GTCGAC 1 cut(s) 158
SapI GCTCTTC 2 cut(s) 42, 821
SaqAI TTAA 3 cut(s) 11, 927, 1213
Sau3AI GATC 3 cut(s) 175, 844, 1162
Sau96I GGNCC 5 cut(s) 256, 282, 357, 358, 397
SchI GAGTC 2 cut(s) 664, 986
ScrFI CCNGG 3 cut(s) 75, 196, 254
SduI GDGCHC 5 cut(s) 108, 195, 361, 406, 871
SexAI ACCWGGT 1 cut(s) 73
SfaNI GCATC 1 cut(s) 1090
SfcI CTRYAG 3 cut(s) 710, 1095, 1185
Sfr303I CCGCGG 1 cut(s) 412
SgrAI CRCCGGYG 1 cut(s) 296
SgrBI CCGCGG 1 cut(s) 412
SinI GGWCC 2 cut(s) 256, 282
SmlI CTYRAG 1 cut(s) 549
SmoI CTYRAG 1 cut(s) 549
Sse9I AATT 1 cut(s) 577
SsiI CCGC 6 cut(s) 153, 409, 411, 445, 472, 1116
SspMI CTAG 1 cut(s) 702
SstI GAGCTC 1 cut(s) 108
StyD4I CCNGG 3 cut(s) 73, 194, 252
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 3 cut(s) 344, 753, 879
TaiI ACGT 2 cut(s) 482, 987
TaqI TCGA 4 cut(s) 159, 174, 450, 1038
TasI AATT 1 cut(s) 577
TauI GCSGC 2 cut(s) 156, 414
TfiI GAWTC 4 cut(s) 715, 961, 1190, 1198
Tru1I TTAA 3 cut(s) 11, 927, 1213
Tru9I TTAA 3 cut(s) 11, 927, 1213
TscAI CASTG 6 cut(s) 349, 358, 546, 807, 862, 884
TseFI GTSAC 1 cut(s) 822
TseI GCWGC 8 cut(s) 602, 633, 794, 797, 893, 932, 1091, 1184
Tsp45I GTSAC 1 cut(s) 822
TspDTI ATGAA 5 cut(s) 386, 504, 749, 774, 998
TspRI CASTG 6 cut(s) 349, 358, 546, 807, 862, 884
VpaK11BI GGWCC 2 cut(s) 256, 282
XcmI CCANNNNNNNNNTGG 1 cut(s) 1055
XmiI GTMKAC 2 cut(s) 135, 159
XspI CTAG 1 cut(s) 702
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.