MD06G1004900.v1.1

Protein CUP-SHAPED COTYLEDON

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0013567
Physical Location & Seq
Forward (+)
536545 .. 541413
4869 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1004900.v1.1.491

Sequence Viewer

Length: 1245 bp
ATGTTGGGAATCGGAATGGAAGAAATTATGAGTGAGCTGATGAGTGGGGAGGATGAAAACGGACAAGGTTTTCAACCAGGGTTCCGGTTCCATCCGACGGACGAGGAGCTCATAACCTTTTATCTGGCTTCCAAGGTCTACAATGGCTCTTTCTGCGGCGTCCGCATTGCTGAGGTCGACCTTAACCGATGCGAGCCATGGGAGCTCCCAGACGTGGCAAAGATGGGAGAGAGAGAGTGGTACTTCTTCAGCCTGAGGGACAGGAAGTACCCAACGGGGATGAGAACCAACAGAGCCACGGGAGCCGGCTACTGGAAGGCCACCGGCAAAGACCGACAGGTCTTCAGTGCGTCGACCGGAGCCCTACTTGGCATGAAGAAGACCCTTGTCTTCTACAAGGGCAGAGCTCCGAGGGGCCACAAGACCAAGTGGGTAATGCATGAGTACCGTCTCGACGGCCACTTCTCCTCCTATCGCCACGACTGCCAGGAGGAATGGGTGATATGCAGAATAAATCACAAATCTGGGAAGAAGAAAAGCCCACTACTTGATCACGAAGCTGCTGCTTCGGCAATTTCAAATTGCAATTATCTGCCTCCATTGCTTGATCCATCTTCAGCATTATTATTGCACGAATCATGCCATGGATATCTGAATCCAATGCAATTGCAAGCTCCTCCTCCTCCTCCAATTCACCAAGAAAATCATGATACCTTCTCCTCCTTCCCAGCAATAAATGCCTTCCAAACCCACCAATTACCCTCTTTCTCCAGATCCCTCCTCTTCAAATCCCTCTTCGATCAAGATTGCAACAGCAGCAGCAGTTCTAGTACTATTCCCAAACAGTGCAAGACTGAGCTCGCCAACTACTTTTCCCGTTTCCAAACACCGGATAACAGTCATAACAATAATAATAGCACTCGCAACAACTTGCATGGCTTGAACTTGGGGGTGGAATTGGAAAAGAATCGTGATCATCAGTACCAGCCAAACGCCCCCTACCATCATCAGCATGATCAATACAATGATCTCCTGCCTTTTGATTATTTGGACTACAGTACTAGTGTGCTGGGTTTCGCATCAGCAGAAGCAGCAGAGGCTACTACTAGTACTAGCACTGCGACTGCTGCTGTTCATGCTCATGACACGTCTCCCTCAGTTGCTTTCAACAGGGCCGGCTTTCAGACCATGTTAGATCTTCCTCCCATCAAACTCCCTGGAGAATCTTGCCGACCCTATGCATAA

Protein Analysis

415

Amino Acids

46.63

Weight (kDa)

6.37

Isoelectric Point (pI)

39.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 26 - 151 1.5e-46 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013567)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G76420
fragaria_vesca FvH4_5g25960 FvH4_5g25960 FvH4_5g25960
malus_domestica MD06G1004900.v1.1
prunus_persica Prupe.5G006200_v2.0.a1
pyrus_communis pycom06g00440 pycom16g26170
rosa_chinensis RchiOBHm_Chr7g0216521
rosa_laevigata RLG00000002564
rosa_multiflora Rmu_sc0009638.1_g000003 Rmu_sc0009638.1_g000004
rosa_roxburghii Rroxscaffold_3G00243140
rosa_rugosa Rorug07G0160700
rosa_samantha Rh7AG300000 Rh7BG291900 Rh7CG318600
rosa_wichuraiana Rw7G025510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 338
AccI GTMKAC 3 cut(s) 138, 177, 353
AciI CCGC 2 cut(s) 156, 163
AclWI GGATC 2 cut(s) 602, 768
AcoI YGGCCR 1 cut(s) 457
AcuI CTGAAG 3 cut(s) 232, 328, 600
AcyI GRCGYC 1 cut(s) 159
AfaI GTAC 7 cut(s) 242, 269, 446, 832, 983, 1060, 1111
AfiI CCNNNNNNNGG 4 cut(s) 97, 214, 312, 889
AflIII ACRYGT 1 cut(s) 1146
AgsI TTSAA 5 cut(s) 74, 579, 787, 943, 1168
AhlI ACTAGT 2 cut(s) 1061, 1106
AjiI CACGTC 2 cut(s) 214, 1149
AjnI CCWGG 3 cut(s) 76, 486, 1216
AluBI AGCT 7 cut(s) 37, 109, 205, 407, 560, 674, 859
AluI AGCT 7 cut(s) 37, 109, 205, 407, 560, 674, 859
Alw21I GWGCWC 4 cut(s) 111, 207, 409, 861
Alw26I GTCTC 2 cut(s) 455, 1155
AlwI GGATC 2 cut(s) 602, 768
AoxI GGCC 4 cut(s) 318, 415, 457, 1173
ApeKI GCWGC 6 cut(s) 560, 563, 816, 819, 1091, 1127
AspS9I GGNCC 2 cut(s) 415, 1173
AsuHPI GGTGA 2 cut(s) 511, 686
AxyI CCTNAGG 1 cut(s) 254
BanII GRGCYC 5 cut(s) 111, 207, 364, 409, 861
BbsI GAAGAC 3 cut(s) 334, 382, 386
Bbv12I GWGCWC 4 cut(s) 111, 207, 409, 861
BbvCI CCTCAGC 1 cut(s) 171
BbvI GCAGC 6 cut(s) 547, 550, 828, 831, 1103, 1114
BccI CCATC 5 cut(s) 99, 217, 619, 1011, 1214
BceAI ACGGC 1 cut(s) 472
BcgI CGANNNNNNTGC 2 cut(s) 545, 579
BciT130I CCWGG 3 cut(s) 78, 488, 1218
BclI TGATCA 3 cut(s) 550, 973, 1015
BcoDI GTCTC 2 cut(s) 455, 1155
BcuI ACTAGT 2 cut(s) 1061, 1106
BfaI CTAG 4 cut(s) 828, 1062, 1107, 1113
BfmI CTRYAG 1 cut(s) 1054
BglII AGATCT 1 cut(s) 1195
BisI GCNGC 7 cut(s) 157, 561, 564, 817, 820, 1092, 1128
BlsI GCNGC 7 cut(s) 158, 562, 565, 818, 821, 1093, 1129
BmcAI AGTACT 3 cut(s) 832, 1060, 1111
Bme1390I CCNGG 3 cut(s) 78, 488, 1218
BmgBI CACGTC 2 cut(s) 214, 1149
BmgT120I GGNCC 2 cut(s) 415, 1173
BmiI GGNNCC 5 cut(s) 83, 89, 304, 361, 416
BmrFI CCNGG 3 cut(s) 78, 488, 1218
BmsI GCATC 2 cut(s) 179, 1088
BoxI GACNNNNGTC 1 cut(s) 386
BpiI GAAGAC 3 cut(s) 334, 382, 386
BpmI CTGGAG 2 cut(s) 754, 1239
Bpu10I CCTNAGC 1 cut(s) 171
BsaHI GRCGYC 1 cut(s) 159
BsaJI CCNNGG 7 cut(s) 77, 132, 197, 297, 410, 643, 1216
BsaWI WCCGGW 3 cut(s) 84, 356, 889
BsaXI ACNNNNNCTCC 2 cut(s) 452, 482
Bsc4I CCNNNNNNNGG 4 cut(s) 97, 214, 312, 889
Bse118I RCCGGY 3 cut(s) 305, 323, 1175
Bse1I ACTGG 1 cut(s) 317
Bse21I CCTNAGG 1 cut(s) 254
Bse3DI GCAATG 2 cut(s) 165, 599
BseBI CCWGG 3 cut(s) 78, 488, 1218
BseDI CCNNGG 7 cut(s) 77, 132, 197, 297, 410, 643, 1216
BseGI GGATG 3 cut(s) 58, 91, 285
BseLI CCNNNNNNNGG 4 cut(s) 97, 214, 312, 889
BseMI GCAATG 2 cut(s) 165, 599
BseMII CTCAG 4 cut(s) 162, 245, 846, 1170
BseNI ACTGG 1 cut(s) 317
BseRI GAGGAG 8 cut(s) 119, 457, 666, 669, 672, 675, 709, 770
BseXI GCAGC 6 cut(s) 547, 550, 828, 831, 1103, 1114
BseYI CCCAGC 2 cut(s) 727, 1069
Bsh1285I CGRYCG 1 cut(s) 357
BshFI GGCC 4 cut(s) 320, 417, 459, 1175
BsiEI CGRYCG 1 cut(s) 357
BsiHKAI GWGCWC 4 cut(s) 111, 207, 409, 861
BsiSI CCGG 6 cut(s) 85, 306, 324, 357, 890, 1176
BslFI GGGAC 1 cut(s) 272
BslI CCNNNNNNNGG 4 cut(s) 97, 214, 312, 889
BsmAI GTCTC 2 cut(s) 455, 1155
BsmBI CGTCTC 2 cut(s) 455, 1155
BsmFI GGGAC 1 cut(s) 272
BsnI GGCC 4 cut(s) 320, 417, 459, 1175
Bsp1286I GDGCHC 5 cut(s) 111, 207, 364, 409, 861
Bsp143I GATC 8 cut(s) 550, 607, 773, 799, 973, 1015, 1027, 1195
Bsp19I CCATGG 2 cut(s) 197, 643
BspACI CCGC 2 cut(s) 156, 163
BspANI GGCC 4 cut(s) 320, 417, 459, 1175
BspCNI CTCAG 4 cut(s) 163, 246, 847, 1169
BspHI TCATGA 2 cut(s) 706, 1141
BspLI GGNNCC 5 cut(s) 83, 89, 304, 361, 416
BspPI GGATC 2 cut(s) 602, 768
BsrDI GCAATG 2 cut(s) 165, 599
BsrFI RCCGGY 3 cut(s) 305, 323, 1175
BsrI ACTGG 1 cut(s) 317
BssAI RCCGGY 3 cut(s) 305, 323, 1175
BssECI CCNNGG 7 cut(s) 77, 132, 197, 297, 410, 643, 1216
BssMI GATC 8 cut(s) 550, 607, 773, 799, 973, 1015, 1027, 1195
BssNI GRCGYC 1 cut(s) 159
BssT1I CCWWGG 3 cut(s) 132, 197, 643
Bst2UI CCWGG 3 cut(s) 78, 488, 1218
Bst4CI ACNGT 4 cut(s) 449, 846, 899, 1058
Bst6I CTCTTC 2 cut(s) 788, 800
BstACI GRCGYC 1 cut(s) 159
BstAPI GCANNNNNTGC 1 cut(s) 737
BstC8I GCNNGC 5 cut(s) 194, 307, 672, 861, 1177
BstDEI CTNAG 4 cut(s) 171, 254, 855, 1156
BstDSI CCRYGG 3 cut(s) 197, 297, 643
BstF5I GGATG 3 cut(s) 58, 91, 285
BstKTI GATC 8 cut(s) 553, 610, 776, 802, 976, 1018, 1030, 1198
BstMAI GTCTC 2 cut(s) 455, 1155
BstMBI GATC 8 cut(s) 550, 607, 773, 799, 973, 1015, 1027, 1195
BstMCI CGRYCG 1 cut(s) 357
BstNI CCWGG 3 cut(s) 78, 488, 1218
BstPAI GACNNNNGTC 1 cut(s) 386
BstSCI CCNGG 3 cut(s) 76, 486, 1216
BstSFI CTRYAG 1 cut(s) 1054
BstV1I GCAGC 6 cut(s) 547, 550, 828, 831, 1103, 1114
BstV2I GAAGAC 3 cut(s) 334, 382, 386
BstX2I RGATCY 2 cut(s) 773, 1195
BstYI RGATCY 2 cut(s) 773, 1195
Bsu36I CCTNAGG 1 cut(s) 254
BsuRI GGCC 4 cut(s) 320, 417, 459, 1175
BtgI CCRYGG 3 cut(s) 197, 297, 643
BtrI CACGTC 2 cut(s) 214, 1149
BtsCI GGATG 3 cut(s) 58, 91, 285
BtsI GCAGTG 1 cut(s) 1116
BtsIMutI CAGTG 3 cut(s) 352, 851, 1116
Cac8I GCNNGC 5 cut(s) 194, 307, 672, 861, 1177
CciI TCATGA 2 cut(s) 706, 1141
Cfr10I RCCGGY 3 cut(s) 305, 323, 1175
Cfr13I GGNCC 2 cut(s) 415, 1173
CseI GACGC 2 cut(s) 148, 339
Csp6I GTAC 7 cut(s) 241, 268, 445, 831, 982, 1059, 1110
CviQI GTAC 7 cut(s) 241, 268, 445, 831, 982, 1059, 1110
DdeI CTNAG 4 cut(s) 171, 254, 855, 1156
DpnI GATC 8 cut(s) 552, 609, 775, 801, 975, 1017, 1029, 1197
DpnII GATC 8 cut(s) 550, 607, 773, 799, 973, 1015, 1027, 1195
DrdI GACNNNNNNGTC 1 cut(s) 338
DseDI GACNNNNNNGTC 1 cut(s) 338
EaeI YGGCCR 1 cut(s) 457
Eam1104I CTCTTC 2 cut(s) 788, 800
EarI CTCTTC 2 cut(s) 788, 800
Ecl136II GAGCTC 4 cut(s) 109, 205, 407, 859
Eco130I CCWWGG 3 cut(s) 132, 197, 643
Eco24I GRGCYC 5 cut(s) 111, 207, 364, 409, 861
Eco32I GATATC 1 cut(s) 650
Eco53kI GAGCTC 4 cut(s) 109, 205, 407, 859
Eco57I CTGAAG 3 cut(s) 232, 328, 600
Eco81I CCTNAGG 1 cut(s) 254
EcoICRI GAGCTC 4 cut(s) 109, 205, 407, 859
EcoRII CCWGG 3 cut(s) 76, 486, 1216
EcoRV GATATC 1 cut(s) 650
EcoT14I CCWWGG 3 cut(s) 132, 197, 643
EcoT22I ATGCAT 2 cut(s) 441, 1243
EcoT38I GRGCYC 5 cut(s) 111, 207, 364, 409, 861
ErhI CCWWGG 3 cut(s) 132, 197, 643
Esp3I CGTCTC 2 cut(s) 455, 1155
FaqI GGGAC 1 cut(s) 272
FbaI TGATCA 3 cut(s) 550, 973, 1015
FblI GTMKAC 3 cut(s) 138, 177, 353
Fnu4HI GCNGC 7 cut(s) 157, 561, 564, 817, 820, 1092, 1128
FokI GGATG 3 cut(s) 65, 78, 292
FriOI GRGCYC 5 cut(s) 111, 207, 364, 409, 861
Fsp4HI GCNGC 7 cut(s) 157, 561, 564, 817, 820, 1092, 1128
FspBI CTAG 4 cut(s) 828, 1062, 1107, 1113
GluI GCNGC 7 cut(s) 157, 561, 564, 817, 820, 1092, 1128
GsaI CCCAGC 2 cut(s) 731, 1073
GsuI CTGGAG 2 cut(s) 754, 1239
HaeIII GGCC 4 cut(s) 320, 417, 459, 1175
HapII CCGG 6 cut(s) 85, 306, 324, 357, 890, 1176
HgaI GACGC 2 cut(s) 148, 339
Hin1I GRCGYC 1 cut(s) 159
HincII GTYRAC 2 cut(s) 178, 354
HindII GTYRAC 2 cut(s) 178, 354
HinfI GANTC 5 cut(s) 9, 635, 655, 967, 1223
HpaII CCGG 6 cut(s) 85, 306, 324, 357, 890, 1176
HphI GGTGA 2 cut(s) 511, 686
Hpy166II GTNNAC 3 cut(s) 139, 178, 354
Hpy188I TCNGA 5 cut(s) 14, 96, 411, 654, 1185
Hpy188III TCNNGA 7 cut(s) 452, 554, 707, 771, 803, 971, 1142
Hpy8I GTNNAC 3 cut(s) 139, 178, 354
Hpy99I CGWCG 3 cut(s) 100, 355, 458
HpyAV CCTTC 4 cut(s) 310, 724, 733, 751
HpyCH4III ACNGT 4 cut(s) 449, 846, 899, 1058
HpyCH4IV ACGT 2 cut(s) 213, 1148
HpyF3I CTNAG 4 cut(s) 171, 254, 855, 1156
HpySE526I ACGT 2 cut(s) 213, 1148
Hsp92I GRCGYC 1 cut(s) 159
KroI GCCGGC 2 cut(s) 305, 1175
KroNI GCCGGC 2 cut(s) 307, 1177
Ksp22I TGATCA 3 cut(s) 550, 973, 1015
Kzo9I GATC 8 cut(s) 550, 607, 773, 799, 973, 1015, 1027, 1195
LmnI GCTCC 7 cut(s) 106, 202, 210, 302, 359, 412, 679
Lsp1109I GCAGC 6 cut(s) 547, 550, 828, 831, 1103, 1114
LweI GCATC 2 cut(s) 179, 1088
MaeI CTAG 4 cut(s) 828, 1062, 1107, 1113
MaeII ACGT 2 cut(s) 213, 1148
MalI GATC 8 cut(s) 552, 609, 775, 801, 975, 1017, 1029, 1197
MboI GATC 8 cut(s) 550, 607, 773, 799, 973, 1015, 1027, 1195
MfeI CAATTG 1 cut(s) 665
MflI RGATCY 2 cut(s) 773, 1195
MhlI GDGCHC 5 cut(s) 111, 207, 364, 409, 861
MluCI AATT 8 cut(s) 24, 573, 580, 586, 665, 690, 755, 956
MmeI TCCRAC 1 cut(s) 119
Mph1103I ATGCAT 2 cut(s) 441, 1243
MroNI GCCGGC 2 cut(s) 305, 1175
MseI TTAA 1 cut(s) 183
MslI CAYNNNNRTG 2 cut(s) 1011, 1140
MspI CCGG 6 cut(s) 85, 306, 324, 357, 890, 1176
MspR9I CCNGG 3 cut(s) 78, 488, 1218
MunI CAATTG 1 cut(s) 665
MvaI CCWGG 3 cut(s) 78, 488, 1218
NaeI GCCGGC 2 cut(s) 307, 1177
NcoI CCATGG 2 cut(s) 197, 643
NdeII GATC 8 cut(s) 550, 607, 773, 799, 973, 1015, 1027, 1195
NgoMIV GCCGGC 2 cut(s) 305, 1175
NlaIV GGNNCC 5 cut(s) 83, 89, 304, 361, 416
NsiI ATGCAT 2 cut(s) 441, 1243
PagI TCATGA 2 cut(s) 706, 1141
PdiI GCCGGC 2 cut(s) 307, 1177
PfeI GAWTC 5 cut(s) 9, 635, 655, 967, 1223
PkrI GCNGC 7 cut(s) 158, 562, 565, 818, 821, 1093, 1129
PshAI GACNNNNGTC 1 cut(s) 386
Psp124BI GAGCTC 4 cut(s) 111, 207, 409, 861
Psp6I CCWGG 3 cut(s) 76, 486, 1216
PspFI CCCAGC 2 cut(s) 727, 1069
PspGI CCWGG 3 cut(s) 76, 486, 1216
PspN4I GGNNCC 5 cut(s) 83, 89, 304, 361, 416
PspPI GGNCC 2 cut(s) 415, 1173
PsuI RGATCY 2 cut(s) 773, 1195
RsaI GTAC 7 cut(s) 242, 269, 446, 832, 983, 1060, 1111
RsaNI GTAC 7 cut(s) 241, 268, 445, 831, 982, 1059, 1110
RseI CAYNNNNRTG 2 cut(s) 1011, 1140
SacI GAGCTC 4 cut(s) 111, 207, 409, 861
SalI GTCGAC 2 cut(s) 176, 352
SaqAI TTAA 1 cut(s) 183
SatI GCNGC 7 cut(s) 157, 561, 564, 817, 820, 1092, 1128
Sau3AI GATC 8 cut(s) 550, 607, 773, 799, 973, 1015, 1027, 1195
Sau96I GGNCC 2 cut(s) 415, 1173
ScaI AGTACT 3 cut(s) 832, 1060, 1111
ScrFI CCNGG 3 cut(s) 78, 488, 1218
SduI GDGCHC 5 cut(s) 111, 207, 364, 409, 861
SfaNI GCATC 2 cut(s) 179, 1088
SfcI CTRYAG 1 cut(s) 1054
SmiMI CAYNNNNRTG 2 cut(s) 1011, 1140
SpeI ACTAGT 2 cut(s) 1061, 1106
Sse9I AATT 8 cut(s) 24, 573, 580, 586, 665, 690, 755, 956
SsiI CCGC 2 cut(s) 156, 163
SspMI CTAG 4 cut(s) 828, 1062, 1107, 1113
SstI GAGCTC 4 cut(s) 111, 207, 409, 861
StyD4I CCNGG 3 cut(s) 76, 486, 1216
StyI CCWWGG 3 cut(s) 132, 197, 643
TaaI ACNGT 4 cut(s) 449, 846, 899, 1058
TaiI ACGT 2 cut(s) 216, 1151
TaqI TCGA 4 cut(s) 177, 353, 453, 798
TaqII GACCGA 1 cut(s) 348
TasI AATT 8 cut(s) 24, 573, 580, 586, 665, 690, 755, 956
TatI WGTACW 3 cut(s) 830, 1058, 1109
TauI GCSGC 1 cut(s) 159
TfiI GAWTC 5 cut(s) 9, 635, 655, 967, 1223
Tru1I TTAA 1 cut(s) 183
Tru9I TTAA 1 cut(s) 183
TscAI CASTG 3 cut(s) 352, 851, 1123
TseI GCWGC 6 cut(s) 560, 563, 816, 819, 1091, 1127
TspDTI ATGAA 3 cut(s) 69, 389, 1124
TspGWI ACGGA 2 cut(s) 75, 113
TspRI CASTG 3 cut(s) 352, 851, 1123
XmiI GTMKAC 3 cut(s) 138, 177, 353
XspI CTAG 4 cut(s) 828, 1062, 1107, 1113
ZrmI AGTACT 3 cut(s) 832, 1060, 1111
Zsp2I ATGCAT 2 cut(s) 441, 1243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.