Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0008543

Physical Location & Seq

Genomic Coordinates Forward (+)

17985068 .. 17991657

Gene Structure 6590 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g26510.t3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3216 bp

ATGGAAGAGATTGGTGCCCAAGTTGCTACCCCGATTTTCCTCCGTCAATCACTTTCGTCTCGATTCTGCGACCCGCCGGCGGCCATGGCCAAGAAGAAGCGGGATCTCCCTTACCAGGCCCCGAACTATCAACACCCGAATTCGCAGACGCTTTTCGGAAACCCGGGTTCCAACAGCTGGAACCCCAACGTTTGGGACTGGGATGCAGTGAGGTTTGTAGCCAGGCCACTGGACACCGAGATGATGGGCGCTTCGAATTCAGAGCCCAGGAGGAAGGAGGAAGCTGCCGGAGGAGCTGTGAAGAGCACGGCTGTGGCTGTGGAGGATGAGGATGAAAGGCTTCAGCTGAATCTGGGTGGTGGTTTGGCTTCTGTGGAGGAGCCGGCGGTGTCCAGGCCGAATAAGAGGGTCCGGTCTGGATCTCCGGGGAATAATGGTGGAAGCTATCCCATGTGCCAGGTTGATGATTGTAAAGAGGATCTGTCGACTGCGAAAGACTATCATCGCCGGCATAAAGTCTGTGAGAGTCACTCTAAGTCCACCAAAGCCCTTGTTGCCAAGCAGATGCAGAGGTTCTGCCAGCAGTGCAGCAGGTTTCACCCCCTTTCAGAGTTCGATGAGGGGAAGAGAAGTTGCAGAAGGAGACTTGCAGGGCATAACAGGCGAAGAAGGAAAACCCAGCCTGAGGATGTTACCTCAAGGCTCACAATCCCTGGTGATGGGGATAACAAAACTAGTGGAAATTTGGATATTGTTAGCTTGCTAGCTGCTATTACTCGTCCACAAGGGAAAACTGATGTCAGAAATACCAACAGCTCGTCAGTACTGGACAGAGAGCAGCTCCTTCAGATTCTTAGTAAGATAAACTCTTTGCCGCTGCCAGTAGACCTGGCTGCCAAGTTGCCGAATCTTGGAAATTTAAATTGGAAAGCTTCAGACCTACTTCCTTTAGATCTTCAAAACAAATTGAATGGAAAAACATCTGTGTCAACCTTGGACTTGATCACTGTTCTTTCAGCTACTCTAGCAACACCTTCTGACACTCTTGCTATATTGTCTCAAAAGAGCAGTCAGAGTAGTGACAGTGAGAAAACTAAGTTAACATGCTCTGACCAGGAGAGGCCTAATTTGCAGAAGAGGTCTCCTCAAGAGTTTCATTCAGCTGGGGGAGAGAGAAGTAGTACTAGCTACCAGTCTCCGGCAGAAGATTCAGACTGCCAGGTTCAAGAGACTCGAGTCAAATTACCTTTACAGCTATTCAGCTCCTCACCCGAGGATGATAGCCCACCAAAACTGGCTTCCTCTAGAAAGTATTTCTCTTCTGACAGTAGTAATCGCACAGAGGAGAGGTCTCCATCATCATCACCTCCAGTGATGCAAACACTGTTTCCCATGAAGAGTATGGCAGAAACTGTCAAGTCGGAGAAACAGTCAATAAGCAAAGAGTGTAATTTGAACTTGGACTATAGCTTGAATGGTGGTAGTAACTTGCCTTTTGATCTCTTTAGAGGGTCAAATAGAGGTGCTGTGAGCTCCTCTATTCAAAATTTTCCACATCAAGCTGGCTACACATCTTCTGGTTCTGATCATTCACCATCTAGTTTGAATTCTGATCCTCAGGATCGTACTGGAAGAATATTGTTTAAACTATTCGATAAGGATCCCAGTCAGTTACCAGGGACACTGCGGACTCAGGTCTATAGTTGGCTTTCCAACAGTCCTTCTGAAATGGAGAGCCACATACGGCCTGGTTGTGTGGTCCTATCAGTTTATGTGTCAATGCCATTTGCTGCCTGGGAGCACCTTGAAGAAAACCTGGTCCAGCATGTGAGTTCTTTGGTTCAAAGTTCAGATTCTGATTTTTGGAGAAGTGGAAGGTTTTTAGTAAATACAGGGAGGCAATTAGCTTCTCATAAAGATGGAAAGATTCGTCTATGCAAAGCATGGAGATCATATAGTTCCCCAGAGTTGATCTCTGTGTCCCCTTTGGCTGTTGTGGGCGGGCAACAAACCTCTCTTTCAATAAGGGGTAGAAATCTGACTAATCATGGCACCAAGATCCATTGCACGTACAAGGGTGGTTACACATCAAAGGAAGTTGGAACGACATATCATGGAACCGCTTATGATGAAATAAATTTGGGGAGCTTTCAAATTCTTGATGCATCTCCCGGTGTTCTAGGTCGCTGTTTCATAGAGGTGGAGAACGGTTTTAAGGGAAACAGCTTTCCTGTGATTATAGCTGATGCTACTATCTGTAGAGAATTGAATCTCATCGAGTCTGAGTTTGATTCGGAGAGAAAGGTTTGTGGTGCAATTTCAGAAGATGAAAATCATGATTATGGGCGGCCCAGGTCAAGGGAGGAAGTTCTGCACTTTCTGAACGAGCTAGGATGGCTCTTCCAAAGGAAACGGATCTCTTCCATGTTTCAAGGGTCCGGCTACTCTCTTAGTCGTTTCAAATTTTTACTCACATTCAGTGTTGAGAGAGACTTTTGCACTGTGGTGAAAACACTTTTAGACATATTGGTAAATTTCGATGGTGATGGGCTGTCAAGAGAGTCATTGGGGATGTTGTCAGATGTCCAGCTGTTAAACCGGGCTGTAAAGAGGAGGTGCAGGAAGATGATTGACTTGCTTATTAATTACTCTGTGATTAGCAGTGACAAGAAATATATTTTCCCTCCAAATCATGCTGGACCGGGTGGCCTGACACCGCTTCATTTGGCTGCTTCTATGTCAAATTCAGAGGATATGATTGATGCTTTGATGAATGACCCAGAAGAGATCGGATTGAGCTGCTGGAATTCCCTTTTGGATGGCAATGGCCAGTCTCCATATGCTTATGCTATGATGAGGAATAATTACTCTTACAACAATCTGGTGGCTCGCAAACTTACTGACAAAAGAAATAGTCAGGTTACCTTAACAATTGGAAATGAGATAGAGCAAACTCATATGGGGATAGAGCTGGAGCGTAGGAGAAGTATCCAATTAAGACAAGGCAGCAGATCATGTGCCAAGTGCGCACTTGCAGCAACAAAATACACCAGGAGGGTTCCTGGTGCTCAAGGCTTGCTTCAGCGTCCATTCATTCATTCTATGCTCGCTATAGCTGCTGTTTGTGTATGTGTCTGCTTGTTCTTGAGAGGTTCCCCGGACATTGGCTCAGTTGCTCCTTTCAAGTGGGAGAATTTGGATTTTGGCACCATTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006355 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009889 GO:0009891 GO:0009893 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016020 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044212 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051704 GO:0051707 GO:0060255 GO:0065007 GO:0071944 GO:0080090 GO:0097159 GO:0098542 GO:0140110 GO:1901363 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SBP

Protein Analysis

1072

Amino Acids

118.59

Weight (kDa)

8.56

Isoelectric Point (pI)

61.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SBP	PF03110	152 - 225	2.2e-30	SBP domain
Ig_SPL7	PF26102	651 - 743	5.1e-23	SPL7-like, immunoglobulin-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008543)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G20980 AT1G76580 AT1G76580 AT1G76580
fragaria_vesca	FvH4_5g26510 FvH4_5g26510 FvH4_5g26510 FvH4_5g26510
malus_domestica	MD00G1031600.v1.1 MD06G1001500.v1.1
prunus_persica	Prupe.5G002300_v2.0.a1
pyrus_communis	pycom06g00160 pycom16g26290
rosa_chinensis	RchiOBHm_Chr7g0217411
rosa_laevigata	RLG00000002479
rosa_multiflora	Rmu_sc0000145.1_g000013
rosa_roxburghii	Rroxscaffold_3G00242100
rosa_rugosa	Rorug07G0169000
rosa_samantha	Rh7AG308600 Rh7BG299300 Rh7CG328000 Rh7DG307900
rosa_wichuraiana	Rw7G026170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	3028
Acc36I	ACCTGC	1 cut(s)	582
AccB1I	GGYRCC	3 cut(s)	14, 2051, 3206
AccB7I	CCANNNNNTGG	2 cut(s)	177, 192
AccI	GTMKAC	2 cut(s)	485, 885
AclI	AACGTT	1 cut(s)	189
AclWI	GGATC	9 cut(s)	111, 427, 486, 1607, 1629, 1655, 1668, 2053, 2423
AcoI	YGGCCR	3 cut(s)	81, 87, 2827
AcuI	CTGAAG	4 cut(s)	326, 830, 918, 3065
AfaI	GTAC	4 cut(s)	825, 1183, 1627, 2072
AhlI	ACTAGT	1 cut(s)	734
AjuI	GAANNNNNNNTTGG	6 cut(s)	907, 939, 1282, 1314, 2798, 2830
AleI	CACNNNNGTG	2 cut(s)	311, 2504
Alw21I	GWGCWC	4 cut(s)	308, 1535, 1803, 3070
Alw26I	GTCTC	9 cut(s)	63, 637, 1062, 1146, 1200, 1223, 1356, 2484, 2838
AlwI	GGATC	9 cut(s)	111, 427, 486, 1607, 1629, 1655, 1668, 2053, 2423
AlwNI	CAGNNNCTG	2 cut(s)	1412, 1856
Ama87I	CYCGRG	3 cut(s)	163, 1233, 1271
ArsI	GACNNNNNNTTYG	2 cut(s)	1054, 1086
AseI	ATTAAT	1 cut(s)	2643
Asp700I	GAANNNNTTC	1 cut(s)	339
AspLEI	GCGC	2 cut(s)	251, 3029
AspS9I	GGNCC	7 cut(s)	118, 409, 1759, 1819, 2349, 2436, 2699
AsuC2I	CCSGG	7 cut(s)	164, 165, 426, 2172, 2600, 2703, 3158
AsuHPI	GGTGA	7 cut(s)	590, 728, 1260, 1356, 1584, 2518, 2555
AsuII	TTCGAA	1 cut(s)	254
AsuNHI	GCTAGC	1 cut(s)	763
AvaI	CYCGRG	3 cut(s)	163, 1233, 1271
AvaII	GGWCC	5 cut(s)	409, 1759, 1819, 2436, 2699
AxyI	CCTNAGG	2 cut(s)	684, 1617
BaeGI	GKGCMC	1 cut(s)	19
BalI	TGGCCA	2 cut(s)	89, 2829
BamHI	GGATCC	1 cut(s)	1660
BanI	GGYRCC	3 cut(s)	14, 2051, 3206
BanII	GRGCYC	2 cut(s)	267, 1535
Bbv12I	GWGCWC	4 cut(s)	308, 1535, 1803, 3070
BccI	CCATC	9 cut(s)	238, 713, 1363, 1603, 1913, 2388, 2534, 2540, 2813
BceAI	ACGGC	2 cut(s)	324, 1760
BciVI	GTATCC	1 cut(s)	2999
BclI	TGATCA	2 cut(s)	1002, 1585
BcnI	CCSGG	7 cut(s)	164, 165, 426, 2172, 2600, 2703, 3158
BcoDI	GTCTC	9 cut(s)	63, 637, 1062, 1146, 1200, 1223, 1356, 2484, 2838
BcuI	ACTAGT	1 cut(s)	734
BfaI	CTAG	8 cut(s)	735, 764, 1025, 1185, 1305, 1599, 2180, 2390
BfmI	CTRYAG	4 cut(s)	1465, 1699, 2257, 3111
BfoI	RGCGCY	1 cut(s)	252
BfuAI	ACCTGC	1 cut(s)	582
BfuI	GTATCC	1 cut(s)	2999
BglII	AGATCT	1 cut(s)	952
BmcAI	AGTACT	2 cut(s)	825, 1183
Bme18I	GGWCC	5 cut(s)	409, 1759, 1819, 2436, 2699
BmeT110I	CYCGRG	3 cut(s)	163, 1233, 1271
BmgT120I	GGNCC	7 cut(s)	118, 409, 1759, 1819, 2349, 2436, 2699
BmrI	ACTGGG	2 cut(s)	208, 1659
BmsI	GCATC	7 cut(s)	193, 555, 1365, 2152, 2174, 2236, 2752
BmtI	GCTAGC	1 cut(s)	767
BmuI	ACTGGG	2 cut(s)	208, 1659
BoxI	GACNNNNGTC	2 cut(s)	1235, 1694
BplI	GAGNNNNNCTC	8 cut(s)	515, 547, 825, 857, 1129, 1161, 1958, 1990
BpmI	CTGGAG	2 cut(s)	1353, 2993
Bpu14I	TTCGAA	1 cut(s)	254
BpuEI	CTTGAG	4 cut(s)	682, 1131, 3054, 3166
BpuMI	CCSGG	7 cut(s)	164, 165, 426, 2172, 2600, 2703, 3158
BsaAI	YACGTR	1 cut(s)	2070
BsaBI	GATNNNNATC	2 cut(s)	1659, 2331
BsaI	GGTCTC	2 cut(s)	1146, 1356
BsaWI	WCCGGW	1 cut(s)	411
BsaXI	ACNNNNNCTCC	4 cut(s)	282, 312, 1415, 1445
Bse118I	RCCGGY	3 cut(s)	76, 382, 507
Bse21I	CCTNAGG	2 cut(s)	684, 1617
Bse3DI	GCAATG	2 cut(s)	2062, 2830
Bse8I	GATNNNNATC	2 cut(s)	1659, 2331
BseGI	GGATG	8 cut(s)	208, 331, 337, 694, 1282, 2399, 2577, 2824
BseJI	GATNNNNATC	2 cut(s)	1659, 2331
BseMI	GCAATG	2 cut(s)	2062, 2830
BseMII	CTCAG	5 cut(s)	675, 1631, 1706, 2274, 3183
BseRI	GAGGAG	7 cut(s)	306, 392, 1134, 1255, 1358, 1525, 2626
BseSI	GKGCMC	1 cut(s)	19
BseYI	CCCAGC	2 cut(s)	678, 1163
BsgI	GTGCAG	3 cut(s)	607, 2357, 2638
BshNI	GGYRCC	3 cut(s)	14, 2051, 3206
BsiHKAI	GWGCWC	4 cut(s)	308, 1535, 1803, 3070
BsiHKCI	CYCGRG	3 cut(s)	163, 1233, 1271
BslFI	GGGAC	3 cut(s)	209, 1693, 1966
BsmAI	GTCTC	9 cut(s)	63, 637, 1062, 1146, 1200, 1223, 1356, 2484, 2838
BsmBI	CGTCTC	1 cut(s)	63
BsmFI	GGGAC	3 cut(s)	209, 1693, 1966
Bso31I	GGTCTC	2 cut(s)	1146, 1356
BsoBI	CYCGRG	3 cut(s)	163, 1233, 1271
Bsp119I	TTCGAA	1 cut(s)	254
Bsp1286I	GDGCHC	6 cut(s)	19, 267, 308, 1535, 1803, 3070
Bsp19I	CCATGG	1 cut(s)	84
BspCNI	CTCAG	5 cut(s)	676, 1630, 1705, 2275, 3182
BspHI	TCATGA	1 cut(s)	2335
BspMI	ACCTGC	1 cut(s)	582
BspOI	GCTAGC	1 cut(s)	767
BspPI	GGATC	9 cut(s)	111, 427, 486, 1607, 1629, 1655, 1668, 2053, 2423
BspQI	GCTCTTC	2 cut(s)	296, 2405
BspT104I	TTCGAA	1 cut(s)	254
BspT107I	GGYRCC	3 cut(s)	14, 2051, 3206
BspTNI	GGTCTC	2 cut(s)	1146, 1356
BsrDI	GCAATG	2 cut(s)	2062, 2830
BsrFI	RCCGGY	3 cut(s)	76, 382, 507
BssAI	RCCGGY	3 cut(s)	76, 382, 507
BssT1I	CCWWGG	2 cut(s)	84, 993
Bst4CI	ACNGT	9 cut(s)	1009, 1085, 1328, 1386, 1414, 1431, 1718, 2210, 2503
Bst6I	CTCTTC	8 cut(s)	296, 620, 1130, 1324, 1391, 2405, 2425, 2778
BstBAI	YACGTR	1 cut(s)	2070
BstBI	TTCGAA	1 cut(s)	254
BstDEI	CTNAG	9 cut(s)	534, 684, 854, 1095, 1617, 1692, 2283, 2450, 3169
BstDSI	CCRYGG	1 cut(s)	84
BstEII	GGTNACC	1 cut(s)	2920
BstF5I	GGATG	8 cut(s)	208, 331, 337, 694, 1282, 2399, 2577, 2824
BstH2I	RGCGCY	1 cut(s)	252
BstHHI	GCGC	2 cut(s)	251, 3029
BstMAI	GTCTC	9 cut(s)	63, 637, 1062, 1146, 1200, 1223, 1356, 2484, 2838
BstNSI	RCATGY	2 cut(s)	1107, 1829
BstPAI	GACNNNNGTC	2 cut(s)	1235, 1694
BstPI	GGTNACC	1 cut(s)	2920
BstSFI	CTRYAG	4 cut(s)	1465, 1699, 2257, 3111
BstSLI	GKGCMC	1 cut(s)	19
BstX2I	RGATCY	7 cut(s)	103, 419, 478, 952, 1660, 2058, 2415
BstXI	CCANNNNNNTGG	1 cut(s)	229
BstYI	RGATCY	7 cut(s)	103, 419, 478, 952, 1660, 2058, 2415
Bsu36I	CCTNAGG	2 cut(s)	684, 1617
BsuI	GTATCC	1 cut(s)	2999
BtgI	CCRYGG	1 cut(s)	84
BtgZI	GCGATG	1 cut(s)	488
BtsCI	GGATG	8 cut(s)	208, 331, 337, 694, 1282, 2399, 2577, 2824
BtsI	GCAGTG	4 cut(s)	213, 590, 1682, 2668
BveI	ACCTGC	1 cut(s)	582
CaiI	CAGNNNCTG	2 cut(s)	1412, 1856
CciI	TCATGA	1 cut(s)	2335
CfoI	GCGC	2 cut(s)	251, 3029
Cfr10I	RCCGGY	3 cut(s)	76, 382, 507
Cfr13I	GGNCC	7 cut(s)	118, 409, 1759, 1819, 2349, 2436, 2699
Cfr9I	CCCGGG	1 cut(s)	163
CseI	GACGC	2 cut(s)	157, 3074
CsiI	ACCWGGT	1 cut(s)	1815
Csp6I	GTAC	4 cut(s)	824, 1182, 1626, 2071
CspCI	CAANNNNNGTGG	2 cut(s)	718, 753
CviQI	GTAC	4 cut(s)	824, 1182, 1626, 2071
DdeI	CTNAG	9 cut(s)	534, 684, 854, 1095, 1617, 1692, 2283, 2450, 3169
DraI	TTTAAA	2 cut(s)	921, 1645
EaeI	YGGCCR	3 cut(s)	81, 87, 2827
Eam1104I	CTCTTC	8 cut(s)	296, 620, 1130, 1324, 1391, 2405, 2425, 2778
EarI	CTCTTC	8 cut(s)	296, 620, 1130, 1324, 1391, 2405, 2425, 2778
Ecl136II	GAGCTC	1 cut(s)	1533
Eco130I	CCWWGG	2 cut(s)	84, 993
Eco147I	AGGCCT	1 cut(s)	1123
Eco24I	GRGCYC	2 cut(s)	267, 1535
Eco31I	GGTCTC	2 cut(s)	1146, 1356
Eco47I	GGWCC	5 cut(s)	409, 1759, 1819, 2436, 2699
Eco53kI	GAGCTC	1 cut(s)	1533
Eco57I	CTGAAG	4 cut(s)	326, 830, 918, 3065
Eco81I	CCTNAGG	2 cut(s)	684, 1617
Eco88I	CYCGRG	3 cut(s)	163, 1233, 1271
Eco91I	GGTNACC	1 cut(s)	2920
EcoICRI	GAGCTC	1 cut(s)	1533
EcoO109I	RGGNCCY	1 cut(s)	118
EcoO65I	GGTNACC	1 cut(s)	2920
EcoRI	GAATTC	4 cut(s)	139, 256, 1606, 2806
EcoT14I	CCWWGG	2 cut(s)	84, 993
EcoT22I	ATGCAT	1 cut(s)	2167
EcoT38I	GRGCYC	2 cut(s)	267, 1535
ErhI	CCWWGG	2 cut(s)	84, 993
Esp3I	CGTCTC	1 cut(s)	63
FalI	AAGNNNNNCTT	2 cut(s)	3063, 3095
FaqI	GGGAC	3 cut(s)	209, 1693, 1966
FauI	CCCGC	3 cut(s)	81, 93, 1994
FauNDI	CATATG	2 cut(s)	2839, 2958
FbaI	TGATCA	2 cut(s)	1002, 1585
FblI	GTMKAC	2 cut(s)	485, 885
FokI	GGATG	8 cut(s)	215, 338, 344, 701, 1289, 2406, 2584, 2831
FriOI	GRGCYC	2 cut(s)	267, 1535
FspAI	RTGCGCAY	1 cut(s)	3028
FspBI	CTAG	8 cut(s)	735, 764, 1025, 1185, 1305, 1599, 2180, 2390
FspI	TGCGCA	1 cut(s)	3028
GlaI	GCGC	2 cut(s)	250, 3028
GsaI	CCCAGC	2 cut(s)	682, 1167
GsuI	CTGGAG	2 cut(s)	1353, 2993
HaeII	RGCGCY	1 cut(s)	252
HgaI	GACGC	2 cut(s)	157, 3074
HhaI	GCGC	2 cut(s)	251, 3029
Hin6I	GCGC	2 cut(s)	249, 3027
HinP1I	GCGC	2 cut(s)	249, 3027
HincII	GTYRAC	3 cut(s)	486, 990, 1101
HindII	GTYRAC	3 cut(s)	486, 990, 1101
HindIII	AAGCTT	1 cut(s)	930
HpaI	GTTAAC	1 cut(s)	1101
HphI	GGTGA	7 cut(s)	590, 728, 1260, 1356, 1584, 2518, 2555
Hpy166II	GTNNAC	6 cut(s)	486, 540, 782, 886, 990, 1101
Hpy8I	GTNNAC	6 cut(s)	486, 540, 782, 886, 990, 1101
HpyAV	CCTTC	7 cut(s)	268, 633, 663, 854, 1044, 1731, 1869
HpyCH4III	ACNGT	9 cut(s)	1009, 1085, 1328, 1386, 1414, 1431, 1718, 2210, 2503
HpyCH4IV	ACGT	2 cut(s)	189, 2069
HpyF3I	CTNAG	9 cut(s)	534, 684, 854, 1095, 1617, 1692, 2283, 2450, 3169
HpySE526I	ACGT	2 cut(s)	189, 2069
HspAI	GCGC	2 cut(s)	249, 3027
KroI	GCCGGC	3 cut(s)	76, 382, 507
KroNI	GCCGGC	3 cut(s)	78, 384, 509
Ksp22I	TGATCA	2 cut(s)	1002, 1585
KspAI	GTTAAC	1 cut(s)	1101
LguI	GCTCTTC	2 cut(s)	296, 2405
LmnI	GCTCC	9 cut(s)	293, 379, 846, 1268, 1538, 1798, 2145, 2974, 3181
LweI	GCATC	7 cut(s)	193, 555, 1365, 2152, 2174, 2236, 2752
MabI	ACCWGGT	1 cut(s)	1815
MaeI	CTAG	8 cut(s)	735, 764, 1025, 1185, 1305, 1599, 2180, 2390
MaeII	ACGT	2 cut(s)	189, 2069
MaeIII	GTNAC	8 cut(s)	527, 691, 1079, 1484, 1671, 2081, 2663, 2920
MfeI	CAATTG	1 cut(s)	2931
MflI	RGATCY	7 cut(s)	103, 419, 478, 952, 1660, 2058, 2415
MhlI	GDGCHC	6 cut(s)	19, 267, 308, 1535, 1803, 3070
MlsI	TGGCCA	2 cut(s)	89, 2829
MluNI	TGGCCA	2 cut(s)	89, 2829
MlyI	GAGTC	6 cut(s)	535, 1225, 1245, 1684, 2288, 2570
MmeI	TCCRAC	4 cut(s)	195, 1401, 1737, 2080
Mox20I	TGGCCA	2 cut(s)	89, 2829
Mph1103I	ATGCAT	1 cut(s)	2167
MreI	CGCCGGCG	1 cut(s)	76
MroNI	GCCGGC	3 cut(s)	76, 382, 507
MroXI	GAANNNNTTC	1 cut(s)	339
MscI	TGGCCA	2 cut(s)	89, 2829
MseI	TTAA	8 cut(s)	920, 1100, 1644, 2214, 2594, 2643, 2927, 2996
MslI	CAYNNNNRTG	6 cut(s)	239, 311, 1917, 2198, 2340, 2504
Msp20I	TGGCCA	2 cut(s)	89, 2829
MspA1I	CMGCKG	5 cut(s)	177, 346, 877, 1163, 2590
MssI	GTTTAAAC	1 cut(s)	1645
MunI	CAATTG	1 cut(s)	2931
NaeI	GCCGGC	3 cut(s)	78, 384, 509
NciI	CCSGG	7 cut(s)	164, 165, 426, 2172, 2600, 2703, 3158
NcoI	CCATGG	1 cut(s)	84
NdeI	CATATG	2 cut(s)	2839, 2958
NgoMIV	GCCGGC	3 cut(s)	76, 382, 507
NheI	GCTAGC	1 cut(s)	763
NmuCI	GTSAC	3 cut(s)	527, 1079, 2663
NsbI	TGCGCA	1 cut(s)	3028
NsiI	ATGCAT	1 cut(s)	2167
NspI	RCATGY	2 cut(s)	1107, 1829
NspV	TTCGAA	1 cut(s)	254
OliI	CACNNNNGTG	2 cut(s)	311, 2504
PaeR7I	CTCGAG	1 cut(s)	1233
PagI	TCATGA	1 cut(s)	2335
PceI	AGGCCT	1 cut(s)	1123
PciSI	GCTCTTC	2 cut(s)	296, 2405
PdiI	GCCGGC	3 cut(s)	78, 384, 509
PdmI	GAANNNNTTC	1 cut(s)	339
PfeI	GAWTC	9 cut(s)	63, 349, 850, 907, 1208, 1853, 1927, 2269, 2291
PflMI	CCANNNNNTGG	2 cut(s)	177, 192
PleI	GAGTC	6 cut(s)	534, 1225, 1244, 1684, 2287, 2569
PmeI	GTTTAAAC	1 cut(s)	1645
PpsI	GAGTC	6 cut(s)	534, 1225, 1244, 1684, 2287, 2569
Ppu21I	YACGTR	1 cut(s)	2070
PshAI	GACNNNNGTC	2 cut(s)	1235, 1694
PshBI	ATTAAT	1 cut(s)	2643
Psp124BI	GAGCTC	1 cut(s)	1535
Psp1406I	AACGTT	1 cut(s)	189
PspEI	GGTNACC	1 cut(s)	2920
PspFI	CCCAGC	2 cut(s)	678, 1163
PspPI	GGNCC	7 cut(s)	118, 409, 1759, 1819, 2349, 2436, 2699
PspXI	VCTCGAGB	1 cut(s)	1233
PstNI	CAGNNNCTG	2 cut(s)	1412, 1856
PsuI	RGATCY	7 cut(s)	103, 419, 478, 952, 1660, 2058, 2415
PvuII	CAGCTG	4 cut(s)	177, 346, 1163, 2590
RsaI	GTAC	4 cut(s)	825, 1183, 1627, 2072
RsaNI	GTAC	4 cut(s)	824, 1182, 1626, 2071
RseI	CAYNNNNRTG	6 cut(s)	239, 311, 1917, 2198, 2340, 2504
SacI	GAGCTC	1 cut(s)	1535
SalI	GTCGAC	1 cut(s)	484
SapI	GCTCTTC	2 cut(s)	296, 2405
SaqAI	TTAA	8 cut(s)	920, 1100, 1644, 2214, 2594, 2643, 2927, 2996
Sau96I	GGNCC	7 cut(s)	118, 409, 1759, 1819, 2349, 2436, 2699
ScaI	AGTACT	2 cut(s)	825, 1183
SchI	GAGTC	6 cut(s)	535, 1225, 1245, 1684, 2288, 2570
SduI	GDGCHC	6 cut(s)	19, 267, 308, 1535, 1803, 3070
SexAI	ACCWGGT	1 cut(s)	1815
SfaNI	GCATC	7 cut(s)	193, 555, 1365, 2152, 2174, 2236, 2752
SfcI	CTRYAG	4 cut(s)	1465, 1699, 2257, 3111
Sfr274I	CTCGAG	1 cut(s)	1233
SfuI	TTCGAA	1 cut(s)	254
SgrAI	CRCCGGYG	1 cut(s)	76
SinI	GGWCC	5 cut(s)	409, 1759, 1819, 2436, 2699
SlaI	CTCGAG	1 cut(s)	1233
SmaI	CCCGGG	1 cut(s)	165
SmiI	ATTTAAAT	1 cut(s)	921
SmiMI	CAYNNNNRTG	6 cut(s)	239, 311, 1917, 2198, 2340, 2504
SmlI	CTYRAG	5 cut(s)	697, 1146, 1233, 3069, 3145
SmoI	CTYRAG	5 cut(s)	697, 1146, 1233, 3069, 3145
SpeI	ACTAGT	1 cut(s)	734
SseBI	AGGCCT	1 cut(s)	1123
SspI	AATATT	1 cut(s)	1638
SspMI	CTAG	8 cut(s)	735, 764, 1025, 1185, 1305, 1599, 2180, 2390
SstI	GAGCTC	1 cut(s)	1535
StuI	AGGCCT	1 cut(s)	1123
StyI	CCWWGG	2 cut(s)	84, 993
SwaI	ATTTAAAT	1 cut(s)	921
TaaI	ACNGT	9 cut(s)	1009, 1085, 1328, 1386, 1414, 1431, 1718, 2210, 2503
TaiI	ACGT	2 cut(s)	192, 2072
TaqI	TCGA	8 cut(s)	61, 254, 485, 615, 1234, 1653, 2277, 2538
TatI	WGTACW	2 cut(s)	823, 1181
TauI	GCSGC	3 cut(s)	83, 877, 2350
TfiI	GAWTC	9 cut(s)	63, 349, 850, 907, 1208, 1853, 1927, 2269, 2291
Tru1I	TTAA	8 cut(s)	920, 1100, 1644, 2214, 2594, 2643, 2927, 2996
Tru9I	TTAA	8 cut(s)	920, 1100, 1644, 2214, 2594, 2643, 2927, 2996
TseFI	GTSAC	3 cut(s)	527, 1079, 2663
Tsp45I	GTSAC	3 cut(s)	527, 1079, 2663
TspGWI	ACGGA	2 cut(s)	32, 2428
TspMI	CCCGGG	1 cut(s)	163
Van91I	CCANNNNNTGG	2 cut(s)	177, 192
VpaK11BI	GGWCC	5 cut(s)	409, 1759, 1819, 2436, 2699
VspI	ATTAAT	1 cut(s)	2643
XbaI	TCTAGA	1 cut(s)	1304
XceI	RCATGY	2 cut(s)	1107, 1829
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1399, 1745, 1791
XhoI	CTCGAG	1 cut(s)	1233
XmaI	CCCGGG	1 cut(s)	163
XmiI	GTMKAC	2 cut(s)	485, 885
XmnI	GAANNNNTTC	1 cut(s)	339
XspI	CTAG	8 cut(s)	735, 764, 1025, 1185, 1305, 1599, 2180, 2390
ZrmI	AGTACT	2 cut(s)	825, 1183
Zsp2I	ATGCAT	1 cut(s)	2167

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.