Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0008543

Physical Location & Seq

Genomic Coordinates Reverse (-)

5420024 .. 5426111

Gene Structure 6088 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1031600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3225 bp

ATGGAAGACGCCGGCGGCCAAGTTGCTGCTCCAATTTACTTTCACCAGACGCTTTCGGGCCGGTTTTGCGATTCGCCGGCCATGGGGAGGAAGCGCGATCACCCTTACCAGGGCCCCAATTATCAACACCCTCACTTGCAGCAGCTGCGCATGACAAACCCCGGCAACAACTGGAATCCAAACGTTTGGGACTGGGACGCTGTGACATTTGTTGCCAAGCCTCTGGACTCCGAGCTGCAGCATCTTGGTACCACTTTCACAACAGAGCAGAGGAACAAAGAGGAAGCTACTGGGCCTGTGAAGAACACGGCGGAAGATGAAGATGATGAGAGCCTTCAGCTGAATCTCGCTGGTGGCTTCACCTCTGTGGAGGAGCCCGTGCCCAGGCCCAATAAAAGGGTCCGGTCTGGATCTCCGGGCAATGGGAATGGGAGCTACCCTATGTGCCAAGTTGATAACTGCAAGGAAGATCTGTCCAATGCGAAGGACTACCACCGCCGACATAAAGTCTGTGAGATTCACAGCAAATCCACCAAATCCCTTGTTGCCAAGCAGATGCAGAGATTCTGCCAGCAGTGCAGCAGGTTTCACCTCCTTTCAGAGTTCGATGAGGGGAAGAGGAGTTGTAGACGGAGACTAGCGGGGCATAACCGGAGAAGAAGGAAAACCCAAGCAGAGGATGTTACTTCACGGCTCACTCTCCCAGGAGGTGGCGATAACAAAGGCATTGGAAATATAGATATCGTCAACTTGCTAGCTGATATAGCTTGTCCACAAGGGAAAAATGATGTCAGAAATATCAATGGCTCATCGGTATTGGACAGAGAGCAGCTCCTTCAGATTCTTAGTAAGATAAATTCGTTACCTTTGCCAGCAGACCTTGCTGCCAAGTTGCCGAATCTTGGAAGTTTGACTAGGAAAGCTTCTGAACTGCTTTCTTTAGATCTGCAAAACAAATTGAATGGAAGAGCATCTGCAGCAACCATGGACTTACTTACTGTACTATCAGCTACTCTAGCAACATCTGCTCCAGAAGCTCTGGCAATGTTATCTCAGAAGTGCAGCCAGAGCAGTGACAGTGAGAAAACTAAGTTGACCTGCTCTGACCAGGCAGCTGGTCCCAATTTGCACAAGATACCTCCACAAGAGATTCCTTCAGCTGGGGGAGAGAGAAGTAGTACAAGTTACCAGTCTCCCATGGAAGATTCTGACTGCCAGGTTCAAGAAACTCGAGTTAATTTACCCTTACAGCTATTTAGCTCCTCACCTGAGAATGACAGCCTGCCAAAACTGGCTTCTTCTAGAAAGTATTTCTCTTCTGACAGTAGTAATCCCACTGAGGATAGGTCACCTTCATCTTCCCCTCCTGTTGTGCATACACTGTTTCCAATGAAGAGTTTGGCAGAAACTGTTAAGTCTGAGAAGTTGTTGGTAAGCAAAGAGATCAGCGCAAACCCCGACTCTAGCAGGACTTGTGGCAGTAACATGCCTTTTGATCTTTTTAGAGGGTCAAATAGGGCAGCTGAAGCTAGCTCCATTCAAAGTTTCCCTAACCAACCTGGCTATACTTCTTCTGGGTCGGACCATTCACCTTCTAGTTTGAATTCTGATGCGCAGGATCGCACAGGAAGGATATTATTTAAGCTATTTGATAAAGATCCAAGTCATTTACCAGGGACATTGCGCACACAGATATACAATTGGCTTTCCAACAGTCCATCTGAAATGGAGAGTTACATAAGGCCTGGTTGTGTCGTTCTATCAGTTTATGTATCAATGCCACCTGCTTCCTGGGAGCAACTTGATGAAAACCTGGTTCAGCATGCCAGTTCTTTGGTTCAAAGTTCAGATTCTGATTTTTGGAGAAGTGGGAGGTTTTTAGTTAATACAGGCATGCAATTAGCATCACACAAAGATGAAAAAATTCGTACATGCAAAGCTTGGAGATCATGTAGTTCTCCAGAGTTGATCTCTGTGGCCCCTTTGGCTGTTGTGGGTGGGCAAGAAACTTCTCTTCTGTTAAGGGGTAGAAATTTGAATACTCTTGGCACCAGGATTCATTGCACGTATATGGGGGGTTACACGTCAAAGGAAGCTACTGGTTCAGCATATCATGGAACGATGTTTGATGAGATAAACTTGGGTAGTTTCCAAATTCATGATGCATCTCCTGGTGTTCTTGGTCGCTGTTTCATAGAGGTGGAAAATGGTTTTAGGGGCAACAGTTTTCCAGTGATAATAGCTGACGCGACAATCTGCAGAGAATTGAAAGTCCTTGAGTCAGTGTTTGATGCTGAGTCAAAAGTATGTGATTTAATATCAGAAGATGAAAGCCATGATTATGGGCGGCCAACATCAAGGGAGGAAGCCCTTCACTTTTTGAATGAGCTAGGATGGCTCTTCCAAAGAAAAAGGATATGTTCCATGCTTCAAGAGCCTCGTTATGCACTCAGCCGTTTTAAATTTTTACTCACATTCAGTGTCGAGAAAGACTGTTGTGCTTTGGTTAAAACACTTCTAGACATTCTATTTGATAGAAACTTTGACAGTGATGAGCTGTCGGGAGGGTCAGTTATGCTGTCAGACATGCAGCTTCTGAACCGGGCAGTAAAAAGGAGATGCAGGAAGATGGTTGACTTGCTCATTAATTATTCTATAGTTAATAGTGACATTGACAAGAGATACATCTTTCCACCTAATCTTGCTGGACCTGGCAGCATGACACCTCTTCATTTGGCTGCTTGTATGTCAAGTACGGATGACATGATTGATGCTTTGACAAATGACCCGCAGGAGATTGGCCTGAACTGCTGGAACTCCCTTTTGGATGCTAGTGGGCAATCTCCGTATGCTTATGCTTTGATGAGGAATAACTACTCTTACAACAACTTGGTGGCTCGTAAGCTTGCAGACAGAAGAAATAGTCAGGTTACTGTCACAATTGGAAATGAGAGAGATCAACCTCAGATGAGTATGGAGCTGGAGCGTAGGACAAATACGCGATCCAGACAAGGTAGTAGATCATGTACCAAGTGTGCAATTGCGGCAACCAAATACAGTAGGAGAGTTCCCGGTGCACAAGGCTTGCTTCAGCGTCCCTTCATTCATTCTATGCTTGCTATAGCTGCTGTTTGTGTGTGTGTGTGCTTGTTCTTGAGAGGCTCCCCAGACATTGGCTTAGTTGCTCCTTTCAAGTGGGAGAATTTGGAGTTTGGCACAATTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006355 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009889 GO:0009891 GO:0009893 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016020 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044212 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051704 GO:0051707 GO:0060255 GO:0065007 GO:0071944 GO:0080090 GO:0097159 GO:0098542 GO:0140110 GO:1901363 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SBP

Protein Analysis

1075

Amino Acids

118.82

Weight (kDa)

7.06

Isoelectric Point (pI)

59.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SBP	PF03110	149 - 222	4.1e-29	SBP domain
Ig_SPL7	PF26102	651 - 757	7.5e-36	SPL7-like, immunoglobulin-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008543)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G20980 AT1G76580 AT1G76580 AT1G76580
fragaria_vesca	FvH4_5g26510 FvH4_5g26510 FvH4_5g26510 FvH4_5g26510
malus_domestica	MD00G1031600.v1.1 MD06G1001500.v1.1
prunus_persica	Prupe.5G002300_v2.0.a1
pyrus_communis	pycom06g00160 pycom16g26290
rosa_chinensis	RchiOBHm_Chr7g0217411
rosa_laevigata	RLG00000002479
rosa_multiflora	Rmu_sc0000145.1_g000013
rosa_roxburghii	Rroxscaffold_3G00242100
rosa_rugosa	Rorug07G0169000
rosa_samantha	Rh7AG308600 Rh7BG299300 Rh7CG328000 Rh7DG307900
rosa_wichuraiana	Rw7G026170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1792
Acc16I	TGCGCA	3 cut(s)	149, 1614, 1685
Acc36I	ACCTGC	3 cut(s)	573, 1106, 1792
Acc65I	GGTACC	1 cut(s)	248
AccB1I	GGYRCC	2 cut(s)	248, 2048
AccB7I	CCANNNNNTGG	2 cut(s)	710, 3173
AccI	GTMKAC	1 cut(s)	628
AccII	CGCG	3 cut(s)	96, 2249, 3001
AciI	CCGC	7 cut(s)	15, 311, 496, 641, 2347, 2789, 3044
AclI	AACGTT	1 cut(s)	183
AclWI	GGATC	4 cut(s)	418, 1626, 1652, 2997
AcoI	YGGCCR	3 cut(s)	16, 78, 2348
AcsI	RAATTY	7 cut(s)	856, 1603, 1923, 2032, 2154, 2462, 3202
AcuI	CTGAAG	5 cut(s)	320, 821, 1140, 1545, 3074
AcyI	GRCGYC	1 cut(s)	9
AfaI	GTAC	6 cut(s)	250, 1002, 1180, 1930, 2755, 3028
AflIII	ACRYGT	1 cut(s)	2082
AhdI	GACNNNNNGTC	1 cut(s)	506
AjiI	CACGTC	1 cut(s)	2085
AjuI	GAANNNNNNNTTGG	4 cut(s)	1279, 1311, 2807, 2839
AleI	CACNNNNGTG	1 cut(s)	365
Alw21I	GWGCWC	1 cut(s)	3079
Alw26I	GTCTC	2 cut(s)	628, 1197
Alw44I	GTGCAC	1 cut(s)	3075
AlwI	GGATC	4 cut(s)	418, 1626, 1652, 2997
AlwNI	CAGNNNCTG	4 cut(s)	145, 1409, 1853, 2596
Ama87I	CYCGRG	1 cut(s)	1230
ApaI	GGGCCC	1 cut(s)	116
ApaLI	GTGCAC	1 cut(s)	3075
ApoI	RAATTY	7 cut(s)	856, 1603, 1923, 2032, 2154, 2462, 3202
ArsI	GACNNNNNNTTYG	2 cut(s)	2513, 2545
AseI	ATTAAT	1 cut(s)	2646
Asp700I	GAANNNNTTC	2 cut(s)	1923, 2370
Asp718I	GGTACC	1 cut(s)	248
AspLEI	GCGC	5 cut(s)	96, 150, 1451, 1615, 1686
AsuC2I	CCSGG	4 cut(s)	162, 417, 2603, 3072
AsuHPI	GGTGA	7 cut(s)	35, 92, 352, 581, 1257, 1341, 1581
AsuNHI	GCTAGC	2 cut(s)	754, 1529
AvaI	CYCGRG	1 cut(s)	1230
AvaII	GGWCC	4 cut(s)	400, 1118, 1582, 2708
BaeGI	GKGCMC	3 cut(s)	116, 384, 3079
BanI	GGYRCC	2 cut(s)	248, 2048
BanII	GRGCYC	2 cut(s)	116, 378
BbsI	GAAGAC	1 cut(s)	12
Bbv12I	GWGCWC	1 cut(s)	3079
BccI	CCATC	3 cut(s)	1726, 2388, 2623
BceAI	ACGGC	3 cut(s)	324, 707, 2439
BcnI	CCSGG	4 cut(s)	162, 417, 2603, 3072
BcoDI	GTCTC	2 cut(s)	628, 1197
BfmI	CTRYAG	5 cut(s)	236, 975, 2257, 2655, 3120
BfuAI	ACCTGC	3 cut(s)	573, 1106, 1792
BglI	GCCNNNNNGGC	1 cut(s)	1985
BglII	AGATCT	2 cut(s)	469, 943
Bme18I	GGWCC	4 cut(s)	400, 1118, 1582, 2708
BmeRI	GACNNNNNGTC	1 cut(s)	506
BmeT110I	CYCGRG	1 cut(s)	1230
BmgBI	CACGTC	1 cut(s)	2085
BmiI	GGNNCC	9 cut(s)	114, 115, 250, 375, 401, 1120, 1980, 2050, 3163
BmrI	ACTGGG	2 cut(s)	202, 300
BmtI	GCTAGC	2 cut(s)	758, 1533
BmuI	ACTGGG	2 cut(s)	202, 300
BpiI	GAAGAC	1 cut(s)	12
BplI	GAGNNNNNCTC	4 cut(s)	816, 848, 1955, 1987
BpmI	CTGGAG	3 cut(s)	1014, 1944, 3002
BpuEI	CTTGAG	2 cut(s)	2297, 3175
BpuMI	CCSGG	4 cut(s)	162, 417, 2603, 3072
BsaAI	YACGTR	1 cut(s)	2067
BsaBI	GATNNNNATC	1 cut(s)	1656
BsaHI	GRCGYC	1 cut(s)	9
BsaJI	CCNNGG	9 cut(s)	81, 109, 160, 383, 703, 984, 1197, 1673, 1791
BsaWI	WCCGGW	2 cut(s)	402, 651
BsaXI	ACNNNNNCTCC	6 cut(s)	1012, 1042, 1936, 1966, 2556, 2586
Bse118I	RCCGGY	3 cut(s)	11, 60, 76
Bse1I	ACTGG	8 cut(s)	176, 197, 295, 1189, 1296, 1827, 2104, 2231
Bse3DI	GCAATG	4 cut(s)	427, 1050, 1679, 2059
Bse8I	GATNNNNATC	1 cut(s)	1656
BseDI	CCNNGG	9 cut(s)	81, 109, 160, 383, 703, 984, 1197, 1673, 1791
BseGI	GGATG	4 cut(s)	685, 2399, 2764, 2833
BseJI	GATNNNNATC	1 cut(s)	1656
BseMI	GCAATG	4 cut(s)	427, 1050, 1679, 2059
BseMII	CTCAG	7 cut(s)	1067, 1260, 1329, 1410, 2286, 2464, 2978
BseNI	ACTGG	8 cut(s)	176, 197, 295, 1189, 1296, 1827, 2104, 2231
BseRI	GAGGAG	3 cut(s)	386, 634, 1252
BseSI	GKGCMC	3 cut(s)	116, 384, 3079
BseYI	CCCAGC	1 cut(s)	1160
BsgI	GTGCAG	2 cut(s)	598, 1081
Bsh1236I	CGCG	3 cut(s)	96, 2249, 3001
BshNI	GGYRCC	2 cut(s)	248, 2048
BsiHKAI	GWGCWC	1 cut(s)	3079
BsiHKCI	CYCGRG	1 cut(s)	1230
BsiSI	CCGG	9 cut(s)	12, 61, 77, 162, 403, 416, 652, 2602, 3072
BslFI	GGGAC	5 cut(s)	203, 209, 1104, 1690, 3081
BsmAI	GTCTC	2 cut(s)	628, 1197
BsmFI	GGGAC	5 cut(s)	203, 209, 1104, 1690, 3081
BsoBI	CYCGRG	1 cut(s)	1230
Bsp120I	GGGCCC	1 cut(s)	112
Bsp1286I	GDGCHC	4 cut(s)	116, 378, 384, 3079
Bsp19I	CCATGG	3 cut(s)	81, 984, 1197
BspACI	CCGC	7 cut(s)	15, 311, 496, 641, 2347, 2789, 3044
BspCNI	CTCAG	7 cut(s)	1066, 1261, 1330, 1411, 2287, 2463, 2977
BspFNI	CGCG	3 cut(s)	96, 2249, 3001
BspHI	TCATGA	1 cut(s)	2158
BspLI	GGNNCC	9 cut(s)	114, 115, 250, 375, 401, 1120, 1980, 2050, 3163
BspMAI	CTGCAG	3 cut(s)	240, 979, 2261
BspMI	ACCTGC	3 cut(s)	573, 1106, 1792
BspOI	GCTAGC	2 cut(s)	758, 1533
BspPI	GGATC	4 cut(s)	418, 1626, 1652, 2997
BspQI	GCTCTTC	2 cut(s)	961, 2405
BspT107I	GGYRCC	2 cut(s)	248, 2048
BsrDI	GCAATG	4 cut(s)	427, 1050, 1679, 2059
BsrFI	RCCGGY	3 cut(s)	11, 60, 76
BsrI	ACTGG	8 cut(s)	176, 197, 295, 1189, 1296, 1827, 2104, 2231
BssAI	RCCGGY	3 cut(s)	11, 60, 76
BssECI	CCNNGG	9 cut(s)	81, 109, 160, 383, 703, 984, 1197, 1673, 1791
BssNI	GRCGYC	1 cut(s)	9
BssT1I	CCWWGG	3 cut(s)	81, 984, 1197
Bst6I	CTCTTC	7 cut(s)	611, 961, 1321, 1388, 2019, 2405, 2733
BstACI	GRCGYC	1 cut(s)	9
BstAPI	GCANNNNNTGC	3 cut(s)	145, 881, 1025
BstBAI	YACGTR	1 cut(s)	2067
BstDSI	CCRYGG	3 cut(s)	81, 984, 1197
BstEII	GGTNACC	1 cut(s)	1347
BstF5I	GGATG	4 cut(s)	685, 2399, 2764, 2833
BstFNI	CGCG	3 cut(s)	96, 2249, 3001
BstHHI	GCGC	5 cut(s)	96, 150, 1451, 1615, 1686
BstMAI	GTCTC	2 cut(s)	628, 1197
BstNSI	RCATGY	5 cut(s)	1489, 1826, 1897, 1935, 2590
BstPI	GGTNACC	1 cut(s)	1347
BstSFI	CTRYAG	5 cut(s)	236, 975, 2257, 2655, 3120
BstSLI	GKGCMC	3 cut(s)	116, 384, 3079
BstUI	CGCG	3 cut(s)	96, 2249, 3001
BstV2I	GAAGAC	1 cut(s)	12
BstX2I	RGATCY	4 cut(s)	410, 469, 943, 1657
BstXI	CCANNNNNNTGG	5 cut(s)	186, 223, 1115, 1834, 2342
BstYI	RGATCY	4 cut(s)	410, 469, 943, 1657
BtgI	CCRYGG	3 cut(s)	81, 984, 1197
BtrI	CACGTC	1 cut(s)	2085
BtsCI	GGATG	4 cut(s)	685, 2399, 2764, 2833
BtsI	GCAGTG	2 cut(s)	581, 1078
BtsIMutI	CAGTG	9 cut(s)	581, 1078, 1084, 1335, 1379, 2238, 2289, 2485, 2554
BveI	ACCTGC	3 cut(s)	573, 1106, 1792
CaiI	CAGNNNCTG	4 cut(s)	145, 1409, 1853, 2596
CciI	TCATGA	1 cut(s)	2158
CfoI	GCGC	5 cut(s)	96, 150, 1451, 1615, 1686
Cfr10I	RCCGGY	3 cut(s)	11, 60, 76
CseI	GACGC	5 cut(s)	17, 58, 206, 2255, 3083
CsiI	ACCWGGT	1 cut(s)	1812
Csp6I	GTAC	6 cut(s)	249, 1001, 1179, 1929, 2754, 3027
CviQI	GTAC	6 cut(s)	249, 1001, 1179, 1929, 2754, 3027
DraI	TTTAAA	1 cut(s)	2461
DriI	GACNNNNNGTC	1 cut(s)	506
EaeI	YGGCCR	3 cut(s)	16, 78, 2348
Eam1104I	CTCTTC	7 cut(s)	611, 961, 1321, 1388, 2019, 2405, 2733
Eam1105I	GACNNNNNGTC	1 cut(s)	506
EarI	CTCTTC	7 cut(s)	611, 961, 1321, 1388, 2019, 2405, 2733
EciI	GGCGGA	1 cut(s)	326
Eco130I	CCWWGG	3 cut(s)	81, 984, 1197
Eco147I	AGGCCT	1 cut(s)	1744
Eco24I	GRGCYC	2 cut(s)	116, 378
Eco32I	GATATC	1 cut(s)	742
Eco47I	GGWCC	4 cut(s)	400, 1118, 1582, 2708
Eco57I	CTGAAG	5 cut(s)	320, 821, 1140, 1545, 3074
Eco88I	CYCGRG	1 cut(s)	1230
Eco91I	GGTNACC	1 cut(s)	1347
EcoO109I	RGGNCCY	2 cut(s)	112, 113
EcoO65I	GGTNACC	1 cut(s)	1347
EcoRI	GAATTC	1 cut(s)	1603
EcoRV	GATATC	1 cut(s)	742
EcoT14I	CCWWGG	3 cut(s)	81, 984, 1197
EcoT22I	ATGCAT	1 cut(s)	2167
EcoT38I	GRGCYC	2 cut(s)	116, 378
ErhI	CCWWGG	3 cut(s)	81, 984, 1197
FalI	AAGNNNNNCTT	2 cut(s)	3072, 3104
FaqI	GGGAC	5 cut(s)	203, 209, 1104, 1690, 3081
FauI	CCCGC	2 cut(s)	634, 2796
FblI	GTMKAC	1 cut(s)	628
FokI	GGATG	4 cut(s)	692, 2406, 2771, 2840
FriOI	GRGCYC	2 cut(s)	116, 378
FspI	TGCGCA	3 cut(s)	149, 1614, 1685
GlaI	GCGC	5 cut(s)	95, 149, 1450, 1614, 1685
GsaI	CCCAGC	1 cut(s)	1164
GsuI	CTGGAG	3 cut(s)	1014, 1944, 3002
HapII	CCGG	9 cut(s)	12, 61, 77, 162, 403, 416, 652, 2602, 3072
HgaI	GACGC	5 cut(s)	17, 58, 206, 2255, 3083
HhaI	GCGC	5 cut(s)	96, 150, 1451, 1615, 1686
Hin1I	GRCGYC	1 cut(s)	9
Hin6I	GCGC	5 cut(s)	94, 148, 1449, 1613, 1684
HinP1I	GCGC	5 cut(s)	94, 148, 1449, 1613, 1684
HincII	GTYRAC	3 cut(s)	748, 1095, 2635
HindII	GTYRAC	3 cut(s)	748, 1095, 2635
HindIII	AAGCTT	3 cut(s)	921, 1938, 2903
HpaII	CCGG	9 cut(s)	12, 61, 77, 162, 403, 416, 652, 2602, 3072
HphI	GGTGA	7 cut(s)	35, 92, 352, 581, 1257, 1341, 1581
Hpy166II	GTNNAC	6 cut(s)	629, 748, 773, 1095, 2635, 3077
Hpy8I	GTNNAC	6 cut(s)	629, 748, 773, 1095, 2635, 3077
HpyCH4IV	ACGT	3 cut(s)	183, 2066, 2084
HpySE526I	ACGT	3 cut(s)	183, 2066, 2084
Hsp92I	GRCGYC	1 cut(s)	9
HspAI	GCGC	5 cut(s)	94, 148, 1449, 1613, 1684
KpnI	GGTACC	1 cut(s)	252
KroI	GCCGGC	2 cut(s)	11, 76
KroNI	GCCGGC	2 cut(s)	13, 78
LguI	GCTCTTC	2 cut(s)	961, 2405
MabI	ACCWGGT	1 cut(s)	1812
MaeII	ACGT	3 cut(s)	183, 2066, 2084
MfeI	CAATTG	3 cut(s)	1699, 2940, 3039
MflI	RGATCY	4 cut(s)	410, 469, 943, 1657
MhlI	GDGCHC	4 cut(s)	116, 378, 384, 3079
MlyI	GAGTC	4 cut(s)	221, 1454, 2288, 2306
MmeI	TCCRAC	2 cut(s)	1560, 1734
Mph1103I	ATGCAT	1 cut(s)	2167
MreI	CGCCGGCG	1 cut(s)	11
MroNI	GCCGGC	2 cut(s)	11, 76
MroXI	GAANNNNTTC	2 cut(s)	1923, 2370
MslI	CAYNNNNRTG	5 cut(s)	365, 1914, 2069, 2198, 2340
MspA1I	CMGCKG	5 cut(s)	145, 340, 1115, 1160, 1523
MspI	CCGG	9 cut(s)	12, 61, 77, 162, 403, 416, 652, 2602, 3072
MunI	CAATTG	3 cut(s)	1699, 2940, 3039
MvnI	CGCG	3 cut(s)	96, 2249, 3001
NaeI	GCCGGC	2 cut(s)	13, 78
NciI	CCSGG	4 cut(s)	162, 417, 2603, 3072
NcoI	CCATGG	3 cut(s)	81, 984, 1197
NgoMIV	GCCGGC	2 cut(s)	11, 76
NheI	GCTAGC	2 cut(s)	754, 1529
NlaIV	GGNNCC	9 cut(s)	114, 115, 250, 375, 401, 1120, 1980, 2050, 3163
NmuCI	GTSAC	5 cut(s)	202, 1073, 1347, 2666, 2935
NsbI	TGCGCA	3 cut(s)	149, 1614, 1685
NsiI	ATGCAT	1 cut(s)	2167
NspI	RCATGY	5 cut(s)	1489, 1826, 1897, 1935, 2590
OliI	CACNNNNGTG	1 cut(s)	365
PaeI	GCATGC	2 cut(s)	1826, 1897
PaeR7I	CTCGAG	1 cut(s)	1230
PagI	TCATGA	1 cut(s)	2158
PaqCI	CACCTGC	1 cut(s)	1792
PceI	AGGCCT	1 cut(s)	1744
PciSI	GCTCTTC	2 cut(s)	961, 2405
PdiI	GCCGGC	2 cut(s)	13, 78
PdmI	GAANNNNTTC	2 cut(s)	1923, 2370
PflMI	CCANNNNNTGG	2 cut(s)	710, 3173
PleI	GAGTC	4 cut(s)	221, 1454, 2287, 2305
PpsI	GAGTC	4 cut(s)	221, 1454, 2287, 2305
Ppu21I	YACGTR	1 cut(s)	2067
PshBI	ATTAAT	1 cut(s)	2646
Psp1406I	AACGTT	1 cut(s)	183
PspEI	GGTNACC	1 cut(s)	1347
PspFI	CCCAGC	1 cut(s)	1160
PspN4I	GGNNCC	9 cut(s)	114, 115, 250, 375, 401, 1120, 1980, 2050, 3163
PspOMI	GGGCCC	1 cut(s)	112
PspXI	VCTCGAGB	1 cut(s)	1230
PstI	CTGCAG	3 cut(s)	240, 979, 2261
PstNI	CAGNNNCTG	4 cut(s)	145, 1409, 1853, 2596
PsuI	RGATCY	4 cut(s)	410, 469, 943, 1657
PvuII	CAGCTG	5 cut(s)	145, 340, 1115, 1160, 1523
RsaI	GTAC	6 cut(s)	250, 1002, 1180, 1930, 2755, 3028
RsaNI	GTAC	6 cut(s)	249, 1001, 1179, 1929, 2754, 3027
RseI	CAYNNNNRTG	5 cut(s)	365, 1914, 2069, 2198, 2340
SapI	GCTCTTC	2 cut(s)	961, 2405
SchI	GAGTC	4 cut(s)	221, 1454, 2288, 2306
SduI	GDGCHC	4 cut(s)	116, 378, 384, 3079
SexAI	ACCWGGT	1 cut(s)	1812
SfcI	CTRYAG	5 cut(s)	236, 975, 2257, 2655, 3120
Sfr274I	CTCGAG	1 cut(s)	1230
SgrAI	CRCCGGYG	1 cut(s)	11
SinI	GGWCC	4 cut(s)	400, 1118, 1582, 2708
SlaI	CTCGAG	1 cut(s)	1230
SmiMI	CAYNNNNRTG	5 cut(s)	365, 1914, 2069, 2198, 2340
SmlI	CTYRAG	3 cut(s)	1230, 2276, 3154
SmoI	CTYRAG	3 cut(s)	1230, 2276, 3154
SphI	GCATGC	2 cut(s)	1826, 1897
SseBI	AGGCCT	1 cut(s)	1744
SsiI	CCGC	7 cut(s)	15, 311, 496, 641, 2347, 2789, 3044
StuI	AGGCCT	1 cut(s)	1744
StyI	CCWWGG	3 cut(s)	81, 984, 1197
TaiI	ACGT	3 cut(s)	186, 2069, 2087
TaqI	TCGA	3 cut(s)	606, 1231, 2484
TatI	WGTACW	2 cut(s)	1000, 1178
TauI	GCSGC	3 cut(s)	18, 2350, 3047
TscAI	CASTG	9 cut(s)	581, 1078, 1084, 1342, 1386, 2238, 2289, 2485, 2554
TseFI	GTSAC	5 cut(s)	202, 1073, 1347, 2666, 2935
Tsp45I	GTSAC	5 cut(s)	202, 1073, 1347, 2666, 2935
TspGWI	ACGGA	3 cut(s)	646, 2771, 2835
TspRI	CASTG	9 cut(s)	581, 1078, 1084, 1342, 1386, 2238, 2289, 2485, 2554
Van91I	CCANNNNNTGG	2 cut(s)	710, 3173
VneI	GTGCAC	1 cut(s)	3075
VpaK11BI	GGWCC	4 cut(s)	400, 1118, 1582, 2708
VspI	ATTAAT	1 cut(s)	2646
XapI	RAATTY	7 cut(s)	856, 1603, 1923, 2032, 2154, 2462, 3202
XbaI	TCTAGA	2 cut(s)	1301, 2518
XceI	RCATGY	5 cut(s)	1489, 1826, 1897, 1935, 2590
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1788
XhoI	CTCGAG	1 cut(s)	1230
XmiI	GTMKAC	1 cut(s)	628
XmnI	GAANNNNTTC	2 cut(s)	1923, 2370
Zsp2I	ATGCAT	1 cut(s)	2167

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.