FvH4_5g30191

Sterol 3-beta-glucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0014580
Physical Location & Seq
Forward (+)
21095159 .. 21104815
9657 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g30191.t1

Sequence Viewer

Length: 1455 bp
ATGGAGGTTGAGTCGGACAGGACGAAACCCATTGCTGTTTTCATGGCTTTCGGTACCAAAGGCGATGTCTATCCAATCGCCGCCATTGCGGCGGCCTTTGCTTCCGACCAGACACAGTACCAAGTTGTTCTTATCACACATTCAGCTCATCAGAGCTTACGTTGTCACTTAGCTGAGAAGCATGTTTCCTATGTTCCAATTTCATCACCACCTGTTCTCACTGTCAATCAGAAACATGAAACAGGCTCAAGCATGGATGGTGACTTCATTGTGATAAATTTCTTTGCTCTGGAAGGCTGGAGTCTTGCAGAGCTTTTTCGTGTCCGCTGCATGGTTGCTGCTCCTTATGTTGTTCCGTACAGTGCTCCTTCATCCTTTGAGCGCCACTTTAAAAAGGAATATCAACAGTTGTATAAATACCTGCAGGAATCTCACCTCAACAAGATTTGTTGGGAAGACGTGATCCACTGGATGTGGCCTCTTTTCACAGAACACTGGGGATCCTGGAGATGTGACGATTTGAATCTGAGTCATTGCCCTTTTACAGATCCAGTAACAGGTCTCCCCACATGGCATGATAGGTCATTGTCTCCCCGACTTTTGTATGGTTTTAGCAAAGAAGTTGTTGAGTGCCCAGATTATTGGCCATTGAGCATTCGGGTTTGTGGCTTTTGGTTTCTGCCTACTGAATGGCAATTTTCATGTAAGAAGTGCAGTGAAATTTCAAGGCTTGTATCTTCAAGGAATCTGTACACAAATGATGAAATGTGCCCAGCTCATGTTCATTTGCAATATTTTCTGAAGACTCCTATGCCTATGCCACCAGTTTTTATTGGCCTGAATTCTATTGGTAGCATGGGATTTTTGAAGAATCCTCAAGCTTTTCTTCGTCTTCTCCAAACTGTACTCCAGATAACAAGTTACCGATTTATCCTTTGTACTGCTGGCCATGAGCCTTTGGATGCAGCAATCCGAGCTATTGCTGCTAATTCATCATTGCACATTACCCACAAACAGATTAATGATGATTGTGTTTCTCTCTTTGAGAATCGATTGTTCTGCTTTTCTGGTTCTGTACCATACACGTGGCTTTTTTCTGGATGTTCAGCTGTGATTCATCATGGGGGAAGTGGAACTACTGCTGCAGGACTACTTGCAGGCATCCCACAGGTACTGTGTCCATTTATGCTAGACCAATTTTATTGGGCTGAAAGAATGTTTTGGCTCGGAGTGGCACCAGAACCGTTGAAAAGAAGCCACTTGCTTCCGGAAGAAAGTGATGATGAAAGTATCGAAGAAGCAGCCAATTTTCTAGCAAGGGTTATACATGATGCATTGTCTCCTGAAATTAGACAACGCGCTGTAGAAATTTCTAAGAGAATATCACTTGAGGATGGAGTCTCAAATGCTGTTAGGTATATCAAGGAAGAGGTTGGATGTTCAAGCTCAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

485

Amino Acids

54.83

Weight (kDa)

5.98

Isoelectric Point (pI)

47.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EryCIII-like_C PF06722 357 - 412 2.2e-11 Erythromycin biosynthesis protein CIII-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014580)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G24750
fragaria_vesca FvH4_5g30191 FvH4_5g30191
malus_domestica MD08G1224800.v1.1
prunus_persica Prupe.1G562100_v2.0.a1
pyrus_communis pycom08g19590
rosa_chinensis RchiOBHm_Chr7g0224451
rosa_laevigata RLG00000001894
rosa_multiflora Rmu_sc0003894.1_g000001
rosa_roxburghii Rroxscaffold_3G00234750
rosa_rugosa Rorug07G0223300 Rorug07G0223400
rosa_samantha Rh7AG379900 Rh7BG359500 Rh7CG399400 Rh7DG369100
rosa_wichuraiana Rw7G031180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 429
Acc65I GGTACC 1 cut(s) 53
AccB1I GGYRCC 2 cut(s) 53, 1234
AccII CGCG 1 cut(s) 1359
AccIII TCCGGA 1 cut(s) 1267
AciI CCGC 4 cut(s) 81, 89, 92, 325
AclWI GGATC 4 cut(s) 457, 495, 508, 542
AcoI YGGCCR 2 cut(s) 644, 946
AcsI RAATTY 4 cut(s) 277, 720, 841, 1368
AcuI CTGAAG 1 cut(s) 821
AcvI CACGTG 1 cut(s) 1086
AfaI GTAC 8 cut(s) 55, 119, 359, 752, 906, 940, 1077, 1173
AfiI CCNNNNNNNGG 2 cut(s) 331, 557
AflIII ACRYGT 1 cut(s) 1083
AgsI TTSAA 6 cut(s) 523, 726, 741, 868, 1249, 1443
AjiI CACGTC 1 cut(s) 460
AjnI CCWGG 1 cut(s) 503
AluBI AGCT 9 cut(s) 146, 156, 173, 313, 776, 881, 977, 1109, 1446
AluI AGCT 9 cut(s) 146, 156, 173, 313, 776, 881, 977, 1109, 1446
Alw21I GWGCWC 1 cut(s) 367
Alw26I GTCTC 4 cut(s) 566, 594, 1344, 1405
AlwI GGATC 4 cut(s) 457, 495, 508, 542
AlwNI CAGNNNCTG 1 cut(s) 1174
Aor13HI TCCGGA 1 cut(s) 1267
AoxI GGCC 5 cut(s) 93, 476, 644, 835, 946
ApeKI GCWGC 6 cut(s) 327, 338, 965, 983, 1142, 1301
ApoI RAATTY 4 cut(s) 277, 720, 841, 1368
ArsI GACNNNNNNTTYG 2 cut(s) 51, 83
AseI ATTAAT 1 cut(s) 1020
Asp718I GGTACC 1 cut(s) 53
AspLEI GCGC 2 cut(s) 384, 1361
AsuHPI GGTGA 3 cut(s) 198, 272, 425
BaeGI GKGCMC 2 cut(s) 635, 773
BalI TGGCCA 2 cut(s) 646, 948
BamHI GGATCC 1 cut(s) 500
BanI GGYRCC 2 cut(s) 53, 1234
BarI GAAGNNNNNNTAC 2 cut(s) 1120, 1152
BbrPI CACGTG 1 cut(s) 1086
BbsI GAAGAC 3 cut(s) 462, 809, 884
Bbv12I GWGCWC 1 cut(s) 367
BbvI GCAGC 6 cut(s) 314, 325, 970, 977, 1129, 1313
BccI CCATC 2 cut(s) 251, 1388
BcgI CGANNNNNNTGC 2 cut(s) 1041, 1075
BciT130I CCWGG 1 cut(s) 505
BcoDI GTCTC 4 cut(s) 566, 594, 1344, 1405
BfaI CTAG 2 cut(s) 1190, 1313
BfmI CTRYAG 3 cut(s) 422, 1143, 1362
BfoI RGCGCY 1 cut(s) 385
BfuAI ACCTGC 1 cut(s) 429
BglI GCCNNNNNGGC 1 cut(s) 89
BisI GCNGC 9 cut(s) 81, 90, 93, 328, 339, 966, 984, 1143, 1302
BlsI GCNGC 9 cut(s) 82, 91, 94, 329, 340, 967, 985, 1144, 1303
Bme1390I CCNGG 1 cut(s) 505
BmgBI CACGTC 1 cut(s) 460
BmiI GGNNCC 3 cut(s) 55, 502, 1236
BmrFI CCNGG 1 cut(s) 505
BmrI ACTGGG 1 cut(s) 505
BmsI GCATC 3 cut(s) 952, 1170, 1321
BmuI ACTGGG 1 cut(s) 505
BpiI GAAGAC 3 cut(s) 462, 809, 884
BpmI CTGGAG 3 cut(s) 319, 526, 893
BpuEI CTTGAG 3 cut(s) 232, 861, 1409
Bsa29I ATCGAT 1 cut(s) 1051
BsaAI YACGTR 1 cut(s) 1086
BsaBI GATNNNNATC 2 cut(s) 69, 522
BsaI GGTCTC 1 cut(s) 566
BsaWI WCCGGW 1 cut(s) 1267
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 2 cut(s) 331, 557
Bse1I ACTGG 4 cut(s) 473, 500, 551, 824
Bse3DI GCAATG 4 cut(s) 30, 84, 532, 995
Bse8I GATNNNNATC 2 cut(s) 69, 522
BseAI TCCGGA 1 cut(s) 1267
BseBI CCWGG 1 cut(s) 505
BseCI ATCGAT 1 cut(s) 1051
BseGI GGATG 8 cut(s) 262, 371, 477, 967, 1106, 1161, 1399, 1442
BseJI GATNNNNATC 2 cut(s) 69, 522
BseLI CCNNNNNNNGG 2 cut(s) 331, 557
BseMI GCAATG 4 cut(s) 30, 84, 532, 995
BseMII CTCAG 2 cut(s) 165, 518
BseNI ACTGG 4 cut(s) 473, 500, 551, 824
BseSI GKGCMC 2 cut(s) 635, 773
BseXI GCAGC 6 cut(s) 314, 325, 970, 977, 1129, 1313
BseYI CCCAGC 1 cut(s) 772
BsgI GTGCAG 1 cut(s) 733
Bsh1236I CGCG 1 cut(s) 1359
BshFI GGCC 5 cut(s) 95, 478, 646, 837, 948
BshNI GGYRCC 2 cut(s) 53, 1234
BshVI ATCGAT 1 cut(s) 1051
BsiHKAI GWGCWC 1 cut(s) 367
BsiSI CCGG 1 cut(s) 1268
BslI CCNNNNNNNGG 2 cut(s) 331, 557
BsmAI GTCTC 4 cut(s) 566, 594, 1344, 1405
BsmI GAATGC 1 cut(s) 654
BsnI GGCC 5 cut(s) 95, 478, 646, 837, 948
Bso31I GGTCTC 1 cut(s) 566
Bsp1286I GDGCHC 3 cut(s) 367, 635, 773
Bsp13I TCCGGA 1 cut(s) 1267
Bsp1407I TGTACA 1 cut(s) 750
Bsp143I GATC 3 cut(s) 462, 500, 547
BspACI CCGC 4 cut(s) 81, 89, 92, 325
BspANI GGCC 5 cut(s) 95, 478, 646, 837, 948
BspCNI CTCAG 2 cut(s) 166, 519
BspDI ATCGAT 1 cut(s) 1051
BspEI TCCGGA 1 cut(s) 1267
BspFNI CGCG 1 cut(s) 1359
BspLI GGNNCC 3 cut(s) 55, 502, 1236
BspMAI CTGCAG 2 cut(s) 426, 1147
BspMI ACCTGC 1 cut(s) 429
BspPI GGATC 4 cut(s) 457, 495, 508, 542
BspT107I GGYRCC 2 cut(s) 53, 1234
BspTNI GGTCTC 1 cut(s) 566
BsrDI GCAATG 4 cut(s) 30, 84, 532, 995
BsrGI TGTACA 1 cut(s) 750
BsrI ACTGG 4 cut(s) 473, 500, 551, 824
BssMI GATC 3 cut(s) 462, 500, 547
Bst2UI CCWGG 1 cut(s) 505
Bst4CI ACNGT 7 cut(s) 117, 223, 362, 408, 904, 1176, 1245
Bst6I CTCTTC 1 cut(s) 1422
BstAUI TGTACA 1 cut(s) 750
BstBAI YACGTR 1 cut(s) 1086
BstC8I GCNNGC 2 cut(s) 946, 1159
BstDEI CTNAG 5 cut(s) 169, 174, 527, 1374, 1447
BstF5I GGATG 8 cut(s) 262, 371, 477, 967, 1106, 1161, 1399, 1442
BstFNI CGCG 1 cut(s) 1359
BstH2I RGCGCY 1 cut(s) 385
BstHHI GCGC 2 cut(s) 384, 1361
BstKTI GATC 3 cut(s) 465, 503, 550
BstMAI GTCTC 4 cut(s) 566, 594, 1344, 1405
BstMBI GATC 3 cut(s) 462, 500, 547
BstMWI GCNNNNNNNGC 5 cut(s) 86, 89, 98, 974, 983
BstNI CCWGG 1 cut(s) 505
BstNSI RCATGY 1 cut(s) 185
BstSCI CCNGG 1 cut(s) 503
BstSFI CTRYAG 3 cut(s) 422, 1143, 1362
BstSLI GKGCMC 2 cut(s) 635, 773
BstUI CGCG 1 cut(s) 1359
BstV1I GCAGC 6 cut(s) 314, 325, 970, 977, 1129, 1313
BstV2I GAAGAC 3 cut(s) 462, 809, 884
BstX2I RGATCY 2 cut(s) 500, 547
BstXI CCANNNNNNTGG 2 cut(s) 642, 1086
BstYI RGATCY 2 cut(s) 500, 547
Bsu15I ATCGAT 1 cut(s) 1051
BsuRI GGCC 5 cut(s) 95, 478, 646, 837, 948
BsuTUI ATCGAT 1 cut(s) 1051
BtgZI GCGATG 1 cut(s) 78
BtrI CACGTC 1 cut(s) 460
BtsCI GGATG 8 cut(s) 262, 371, 477, 967, 1106, 1161, 1399, 1442
BtsI GCAGTG 1 cut(s) 721
BtsIMutI CAGTG 6 cut(s) 219, 367, 466, 493, 721, 1455
BveI ACCTGC 1 cut(s) 429
Cac8I GCNNGC 2 cut(s) 946, 1159
CaiI CAGNNNCTG 1 cut(s) 1174
CfoI GCGC 2 cut(s) 384, 1361
ClaI ATCGAT 1 cut(s) 1051
Csp6I GTAC 8 cut(s) 54, 118, 358, 751, 905, 939, 1076, 1172
CviQI GTAC 8 cut(s) 54, 118, 358, 751, 905, 939, 1076, 1172
DdeI CTNAG 5 cut(s) 169, 174, 527, 1374, 1447
DpnI GATC 3 cut(s) 464, 502, 549
DpnII GATC 3 cut(s) 462, 500, 547
DraI TTTAAA 1 cut(s) 391
EaeI YGGCCR 2 cut(s) 644, 946
Eam1104I CTCTTC 1 cut(s) 1422
EarI CTCTTC 1 cut(s) 1422
Eco31I GGTCTC 1 cut(s) 566
Eco57I CTGAAG 1 cut(s) 821
Eco72I CACGTG 1 cut(s) 1086
EcoRI GAATTC 1 cut(s) 841
EcoRII CCWGG 1 cut(s) 503
EcoT22I ATGCAT 1 cut(s) 1336
FalI AAGNNNNNCTT 4 cut(s) 114, 146, 870, 902
Fnu4HI GCNGC 9 cut(s) 81, 90, 93, 328, 339, 966, 984, 1143, 1302
FokI GGATG 8 cut(s) 269, 358, 484, 974, 1113, 1148, 1406, 1449
Fsp4HI GCNGC 9 cut(s) 81, 90, 93, 328, 339, 966, 984, 1143, 1302
FspBI CTAG 2 cut(s) 1190, 1313
GlaI GCGC 2 cut(s) 383, 1360
GluI GCNGC 9 cut(s) 81, 90, 93, 328, 339, 966, 984, 1143, 1302
GsaI CCCAGC 1 cut(s) 776
GsuI CTGGAG 3 cut(s) 319, 526, 893
HaeII RGCGCY 1 cut(s) 385
HaeIII GGCC 5 cut(s) 95, 478, 646, 837, 948
HapII CCGG 1 cut(s) 1268
HhaI GCGC 2 cut(s) 384, 1361
Hin6I GCGC 2 cut(s) 382, 1359
HinP1I GCGC 2 cut(s) 382, 1359
HindIII AAGCTT 1 cut(s) 879
HpaII CCGG 1 cut(s) 1268
HphI GGTGA 3 cut(s) 198, 272, 425
Hpy166II GTNNAC 1 cut(s) 753
Hpy188I TCNGA 8 cut(s) 16, 106, 153, 231, 528, 801, 974, 1229
Hpy188III TCNNGA 5 cut(s) 290, 910, 1098, 1268, 1343
Hpy8I GTNNAC 1 cut(s) 753
HpyAV CCTTC 2 cut(s) 287, 378
HpyCH4III ACNGT 7 cut(s) 117, 223, 362, 408, 904, 1176, 1245
HpyCH4IV ACGT 3 cut(s) 160, 459, 1085
HpyF10VI GCNNNNNNNGC 5 cut(s) 86, 89, 98, 974, 983
HpyF3I CTNAG 5 cut(s) 169, 174, 527, 1374, 1447
HpySE526I ACGT 3 cut(s) 160, 459, 1085
HspAI GCGC 2 cut(s) 382, 1359
Kpn2I TCCGGA 1 cut(s) 1267
KpnI GGTACC 1 cut(s) 57
Kzo9I GATC 3 cut(s) 462, 500, 547
LmnI GCTCC 2 cut(s) 346, 370
Lsp1109I GCAGC 6 cut(s) 314, 325, 970, 977, 1129, 1313
LweI GCATC 3 cut(s) 952, 1170, 1321
MaeI CTAG 2 cut(s) 1190, 1313
MaeII ACGT 3 cut(s) 160, 459, 1085
MaeIII GTNAC 5 cut(s) 164, 260, 512, 553, 920
MalI GATC 3 cut(s) 464, 502, 549
MboI GATC 3 cut(s) 462, 500, 547
MboII GAAGA 9 cut(s) 467, 729, 814, 878, 880, 884, 1283, 1307, 1439
MflI RGATCY 2 cut(s) 500, 547
MhlI GDGCHC 3 cut(s) 367, 635, 773
MlsI TGGCCA 2 cut(s) 646, 948
MluNI TGGCCA 2 cut(s) 646, 948
MlyI GAGTC 5 cut(s) 20, 310, 538, 799, 1407
MmeI TCCRAC 2 cut(s) 129, 1414
MnlI CCTC 5 cut(s) 446, 489, 885, 1384, 1423
Mox20I TGGCCA 2 cut(s) 646, 948
Mph1103I ATGCAT 1 cut(s) 1336
MroI TCCGGA 1 cut(s) 1267
MscI TGGCCA 2 cut(s) 646, 948
MseI TTAA 2 cut(s) 390, 1020
MslI CAYNNNNRTG 1 cut(s) 1084
Msp20I TGGCCA 2 cut(s) 646, 948
MspA1I CMGCKG 2 cut(s) 327, 1109
MspI CCGG 1 cut(s) 1268
MspR9I CCNGG 1 cut(s) 505
Mva1269I GAATGC 1 cut(s) 654
MvaI CCWGG 1 cut(s) 505
MvnI CGCG 1 cut(s) 1359
MwoI GCNNNNNNNGC 5 cut(s) 86, 89, 98, 974, 983
NdeII GATC 3 cut(s) 462, 500, 547
NlaIV GGNNCC 3 cut(s) 55, 502, 1236
NmuCI GTSAC 3 cut(s) 164, 260, 512
NsiI ATGCAT 1 cut(s) 1336
NspI RCATGY 1 cut(s) 185
PcsI WCGNNNNNNNCGW 1 cut(s) 20
PctI GAATGC 1 cut(s) 654
PfeI GAWTC 6 cut(s) 428, 523, 745, 871, 1048, 1114
PfoI TCCNGGA 1 cut(s) 503
PkrI GCNGC 9 cut(s) 82, 91, 94, 329, 340, 967, 985, 1144, 1303
PleI GAGTC 5 cut(s) 19, 309, 537, 799, 1406
PmaCI CACGTG 1 cut(s) 1086
PmlI CACGTG 1 cut(s) 1086
PpsI GAGTC 5 cut(s) 19, 309, 537, 799, 1406
Ppu21I YACGTR 1 cut(s) 1086
PshBI ATTAAT 1 cut(s) 1020
Psp6I CCWGG 1 cut(s) 503
PspCI CACGTG 1 cut(s) 1086
PspFI CCCAGC 1 cut(s) 772
PspGI CCWGG 1 cut(s) 503
PspN4I GGNNCC 3 cut(s) 55, 502, 1236
PstI CTGCAG 2 cut(s) 426, 1147
PstNI CAGNNNCTG 1 cut(s) 1174
PsuI RGATCY 2 cut(s) 500, 547
PvuII CAGCTG 1 cut(s) 1109
RsaI GTAC 8 cut(s) 55, 119, 359, 752, 906, 940, 1077, 1173
RsaNI GTAC 8 cut(s) 54, 118, 358, 751, 905, 939, 1076, 1172
RseI CAYNNNNRTG 1 cut(s) 1084
SaqAI TTAA 2 cut(s) 390, 1020
SatI GCNGC 9 cut(s) 81, 90, 93, 328, 339, 966, 984, 1143, 1302
Sau3AI GATC 3 cut(s) 462, 500, 547
SbfI CCTGCAGG 1 cut(s) 426
SchI GAGTC 5 cut(s) 20, 310, 538, 799, 1407
ScrFI CCNGG 1 cut(s) 505
SdaI CCTGCAGG 1 cut(s) 426
SduI GDGCHC 3 cut(s) 367, 635, 773
SfaNI GCATC 3 cut(s) 952, 1170, 1321
SfcI CTRYAG 3 cut(s) 422, 1143, 1362
SmiMI CAYNNNNRTG 1 cut(s) 1084
SmlI CTYRAG 3 cut(s) 247, 876, 1388
SmoI CTYRAG 3 cut(s) 247, 876, 1388
Sse8387I CCTGCAGG 1 cut(s) 426
SsiI CCGC 4 cut(s) 81, 89, 92, 325
SspI AATATT 1 cut(s) 794
SspMI CTAG 2 cut(s) 1190, 1313
StyD4I CCNGG 1 cut(s) 503
TaaI ACNGT 7 cut(s) 117, 223, 362, 408, 904, 1176, 1245
TaiI ACGT 3 cut(s) 163, 462, 1088
TaqI TCGA 2 cut(s) 1051, 1293
TatI WGTACW 3 cut(s) 750, 904, 938
TauI GCSGC 3 cut(s) 83, 92, 95
TfiI GAWTC 6 cut(s) 428, 523, 745, 871, 1048, 1114
Tru1I TTAA 2 cut(s) 390, 1020
Tru9I TTAA 2 cut(s) 390, 1020
TscAI CASTG 6 cut(s) 226, 367, 473, 500, 721, 1455
TseFI GTSAC 3 cut(s) 164, 260, 512
TseI GCWGC 6 cut(s) 327, 338, 965, 983, 1142, 1301
Tsp45I GTSAC 3 cut(s) 164, 260, 512
TspGWI ACGGA 1 cut(s) 345
TspRI CASTG 6 cut(s) 226, 367, 473, 500, 721, 1455
VspI ATTAAT 1 cut(s) 1020
XapI RAATTY 4 cut(s) 277, 720, 841, 1368
XceI RCATGY 1 cut(s) 185
XspI CTAG 2 cut(s) 1190, 1313
Zsp2I ATGCAT 1 cut(s) 1336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.