Rroxscaffold_3G00234750

Sterol 3-beta-glucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0014580
Physical Location & Seq
Reverse (-)
21363932 .. 21374023
10092 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00234750.1

Sequence Viewer

Length: 1563 bp
ATGGAGGCTGAGACGGAGAGGACGAAACCCGTTGCTGTATTCATGGCTTTCGGTACCAAAGGCGATGTCTATCCAATTGCTGCCATTGCGGCGGCCTTTGCTTCCGACCAGAGACAGTACCAAGTTGTTCTTATCACACACTCAGCTCACGAGAGCTTACGTTGTCACTTGGCTGAGAAGCATGTTTCCTATCTTCCAATTTCAACACCACCTGTTCTCTCCATCCATCAGAAACATGATACTACAGGCTCTGTAGAGTTGCCTCTTTCGCTGCAGAAAAAGCTGTTTTCTGGAGAACATAGAAAAGAATGTCTTTCGGTAGTTGAAACGATATTTGGATCAGGCCCAAGCATGGATGGTGATTTCATTGTGATAAATTTCTTTGCTCTGGAAGGCTGGAGTCTTGCGGAGCTTTTCCGTGTCCGCTGCATGGTTGCTGCTCCTTATGTTGTTCCGTACAGTGCACCTTCATCATTTGAGCGCCAGTTTAAAAAGGAATATCAACACTTGTATAGATACCTGCAGGAACCTAACCTCAGGAAGGTTGGTTGGAAAGACGTGATCCACTGGATGTGGCCTCTTTTCACTGAACACTGGGGATCCTGGAGATGTGACCATTTGAATCTGAGTGCTTTCCCTTTTACAGATCCAGTAACGGGTCTCCCCACTTGGCATGACAGGTCACTGTCTCCCCGACTTTTGTATGGCTTTAGCAAAGAAGTTGTTGAGTGCCCAGATTACTGGCCATCGAGCATTCGGGTTTGTGGCTTTTGGTTTCTGCCTAATGAATGGCAATTTTCATGTAAGAAGTGCAGTGAAATTTCAAGGCTTGTTTCTTCAGGGGATCTGTACACAAATGCTGAACTGTGCCCAGCTCATGTTCACTTGCAATATTTTCTGAAGACTCCTACGCCTATGCCACCTGTTTTTATTGGCCTGAATTCTATTGTTTATAAGTACATGGGATTTTTGAAGAATCCTCAAGCATTTCTTCGTATCCTTGAATCTGTCCTCAAGATAACAACTTATCGTTTTATCCTTTTTACTGCTGGCTACAAGCCTTTAGATGCAGCAATCCGAGCTATTGCTGCTGATTCATCATCGCACATGACCCACAAACAGATTAATGACGATTGCGTTTCTCTCTTCCACGAACGATTGTTCTGCTTTTCTGGTTCTATACCATACACGTGGCTGTTTTCTGGATGTGCGGCTGCAATTCATCATGGGGGAAGTAGTGGAACTACTGCTGCAGCACTACTTGCAGGAATCCCACAGGTACTATGCCCATTTATGCTAGACCAGTTTTATTGGGCAGAAAGAATGTTTTGGCTTGGAGTGGCACCAGAACCGTTGAAAAGGAGCCACTTGCTTCCTGAAGAAAAGAACGACGGAGTCTCAAATGCTGTTAGAGTTTTGAAACGCCTAAAAGATACTACCTTGGATTCTCAGGAGAAGTTCCACTCGAATGAGATCTCCGAAAAAGCTGCTCAGACCAATGGTGTCTCAGTCTCAGGATCAATCATATGCTATAGGCTAAATCAGAGAATGAGAGATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

520

Amino Acids

58.99

Weight (kDa)

7.9

Isoelectric Point (pI)

36.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EryCIII-like_C PF06722 393 - 447 3.2e-10 Erythromycin biosynthesis protein CIII-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014580)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G24750
fragaria_vesca FvH4_5g30191 FvH4_5g30191
malus_domestica MD08G1224800.v1.1
prunus_persica Prupe.1G562100_v2.0.a1
pyrus_communis pycom08g19590
rosa_chinensis RchiOBHm_Chr7g0224451
rosa_laevigata RLG00000001894
rosa_multiflora Rmu_sc0003894.1_g000001
rosa_roxburghii Rroxscaffold_3G00234750
rosa_rugosa Rorug07G0223300 Rorug07G0223400
rosa_samantha Rh7AG379900 Rh7BG359500 Rh7CG399400 Rh7DG369100
rosa_wichuraiana Rw7G031180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 954
Acc36I ACCTGC 1 cut(s) 528
Acc65I GGTACC 1 cut(s) 53
AccB1I GGYRCC 2 cut(s) 53, 1342
AciI CCGC 5 cut(s) 89, 92, 407, 424, 1211
AclWI GGATC 7 cut(s) 346, 556, 594, 607, 641, 852, 1525
AcoI YGGCCR 1 cut(s) 743
AcsI RAATTY 3 cut(s) 376, 819, 940
AcuI CTGAAG 3 cut(s) 822, 920, 1398
AcvI CACGTG 1 cut(s) 1191
AfaI GTAC 6 cut(s) 55, 119, 458, 851, 959, 1281
AfiI CCNNNNNNNGG 4 cut(s) 352, 430, 541, 656
AflIII ACRYGT 1 cut(s) 1188
AgsI TTSAA 8 cut(s) 204, 326, 622, 825, 973, 1004, 1357, 1420
AjiI CACGTC 1 cut(s) 559
AjnI CCWGG 1 cut(s) 602
AjuI GAANNNNNNNTTGG 2 cut(s) 318, 350
AluBI AGCT 7 cut(s) 146, 156, 283, 412, 875, 1082, 1487
AluI AGCT 7 cut(s) 146, 156, 283, 412, 875, 1082, 1487
Alw21I GWGCWC 1 cut(s) 466
Alw26I GTCTC 7 cut(s) 5, 106, 665, 693, 1402, 1510, 1516
Alw44I GTGCAC 1 cut(s) 462
AlwI GGATC 7 cut(s) 346, 556, 594, 607, 641, 852, 1525
AlwNI CAGNNNCTG 1 cut(s) 251
AoxI GGCC 5 cut(s) 93, 343, 575, 743, 934
ApaLI GTGCAC 1 cut(s) 462
ApoI RAATTY 3 cut(s) 376, 819, 940
ArsI GACNNNNNNTTYG 2 cut(s) 51, 83
AseI ATTAAT 1 cut(s) 1125
Asp718I GGTACC 1 cut(s) 53
AspLEI GCGC 1 cut(s) 483
AspS9I GGNCC 1 cut(s) 344
AsuHPI GGTGA 1 cut(s) 371
AxyI CCTNAGG 1 cut(s) 536
BaeGI GKGCMC 3 cut(s) 466, 734, 872
BalI TGGCCA 1 cut(s) 745
BamHI GGATCC 1 cut(s) 599
BanI GGYRCC 2 cut(s) 53, 1342
BauI CACGAG 1 cut(s) 149
BbrPI CACGTG 1 cut(s) 1191
BbsI GAAGAC 1 cut(s) 908
Bbv12I GWGCWC 1 cut(s) 466
BccI CCATC 4 cut(s) 230, 234, 350, 754
BcgI CGANNNNNNTGC 4 cut(s) 1146, 1180, 1469, 1503
BciT130I CCWGG 1 cut(s) 604
BciVI GTATCC 1 cut(s) 1007
BcoDI GTCTC 7 cut(s) 5, 106, 665, 693, 1402, 1510, 1516
BfaI CTAG 1 cut(s) 1298
BfmI CTRYAG 6 cut(s) 243, 252, 272, 521, 1251, 1531
BfoI RGCGCY 1 cut(s) 484
BfuAI ACCTGC 1 cut(s) 528
BfuI GTATCC 1 cut(s) 1007
BglI GCCNNNNNGGC 1 cut(s) 89
BglII AGATCT 1 cut(s) 1473
Bme1390I CCNGG 1 cut(s) 604
BmgBI CACGTC 1 cut(s) 559
BmgT120I GGNCC 1 cut(s) 344
BmiI GGNNCC 5 cut(s) 55, 528, 601, 1344, 1364
BmrFI CCNGG 1 cut(s) 604
BmrI ACTGGG 1 cut(s) 604
BmsI GCATC 1 cut(s) 1057
BmuI ACTGGG 1 cut(s) 604
BpiI GAAGAC 1 cut(s) 908
BpmI CTGGAG 3 cut(s) 312, 418, 625
BpuEI CTTGAG 2 cut(s) 966, 998
BsaAI YACGTR 1 cut(s) 1191
BsaBI GATNNNNATC 1 cut(s) 69
BsaI GGTCTC 1 cut(s) 665
BsaJI CCNNGG 1 cut(s) 1440
Bsc4I CCNNNNNNNGG 4 cut(s) 352, 430, 541, 656
Bse1I ACTGG 6 cut(s) 484, 572, 599, 650, 746, 1303
Bse21I CCTNAGG 1 cut(s) 536
Bse3DI GCAATG 1 cut(s) 84
Bse8I GATNNNNATC 1 cut(s) 69
BseBI CCWGG 1 cut(s) 604
BseDI CCNNGG 1 cut(s) 1440
BseGI GGATG 4 cut(s) 222, 361, 576, 1211
BseJI GATNNNNATC 1 cut(s) 69
BseLI CCNNNNNNNGG 4 cut(s) 352, 430, 541, 656
BseMI GCAATG 1 cut(s) 84
BseMII CTCAG 8 cut(s) 156, 165, 550, 617, 1463, 1505, 1521, 1527
BseNI ACTGG 6 cut(s) 484, 572, 599, 650, 746, 1303
BseSI GKGCMC 3 cut(s) 466, 734, 872
BseYI CCCAGC 1 cut(s) 871
BsgI GTGCAG 1 cut(s) 832
BshFI GGCC 5 cut(s) 95, 345, 577, 745, 936
BshNI GGYRCC 2 cut(s) 53, 1342
BsiHKAI GWGCWC 1 cut(s) 466
BslI CCNNNNNNNGG 4 cut(s) 352, 430, 541, 656
BsmAI GTCTC 7 cut(s) 5, 106, 665, 693, 1402, 1510, 1516
BsmBI CGTCTC 1 cut(s) 5
BsmI GAATGC 1 cut(s) 753
BsnI GGCC 5 cut(s) 95, 345, 577, 745, 936
Bso31I GGTCTC 1 cut(s) 665
Bsp1286I GDGCHC 3 cut(s) 466, 734, 872
Bsp1407I TGTACA 1 cut(s) 849
Bsp143I GATC 7 cut(s) 338, 561, 599, 646, 844, 1473, 1517
BspACI CCGC 5 cut(s) 89, 92, 407, 424, 1211
BspANI GGCC 5 cut(s) 95, 345, 577, 745, 936
BspCNI CTCAG 8 cut(s) 155, 166, 549, 618, 1462, 1504, 1520, 1526
BspHI TCATGA 1 cut(s) 1559
BspLI GGNNCC 5 cut(s) 55, 528, 601, 1344, 1364
BspMAI CTGCAG 3 cut(s) 276, 525, 1255
BspMI ACCTGC 1 cut(s) 528
BspPI GGATC 7 cut(s) 346, 556, 594, 607, 641, 852, 1525
BspT107I GGYRCC 2 cut(s) 53, 1342
BspTNI GGTCTC 1 cut(s) 665
BsrDI GCAATG 1 cut(s) 84
BsrGI TGTACA 1 cut(s) 849
BsrI ACTGG 6 cut(s) 484, 572, 599, 650, 746, 1303
BssECI CCNNGG 1 cut(s) 1440
BssMI GATC 7 cut(s) 338, 561, 599, 646, 844, 1473, 1517
BssSI CACGAG 1 cut(s) 149
BssT1I CCWWGG 1 cut(s) 1440
Bst2BI CACGAG 1 cut(s) 149
Bst2UI CCWGG 1 cut(s) 604
Bst4CI ACNGT 5 cut(s) 117, 461, 687, 867, 1353
Bst6I CTCTTC 1 cut(s) 1151
BstAPI GCANNNNNTGC 1 cut(s) 1262
BstAUI TGTACA 1 cut(s) 849
BstBAI YACGTR 1 cut(s) 1191
BstC8I GCNNGC 1 cut(s) 1051
BstDEI CTNAG 9 cut(s) 9, 142, 174, 536, 626, 1449, 1491, 1507, 1513
BstENI CCTNNNNNAGG 1 cut(s) 539
BstF5I GGATG 4 cut(s) 222, 361, 576, 1211
BstH2I RGCGCY 1 cut(s) 484
BstHHI GCGC 1 cut(s) 483
BstKTI GATC 7 cut(s) 341, 564, 602, 649, 847, 1476, 1520
BstMAI GTCTC 7 cut(s) 5, 106, 665, 693, 1402, 1510, 1516
BstMBI GATC 7 cut(s) 338, 561, 599, 646, 844, 1473, 1517
BstMWI GCNNNNNNNGC 8 cut(s) 86, 89, 98, 268, 280, 1079, 1088, 1262
BstNI CCWGG 1 cut(s) 604
BstNSI RCATGY 1 cut(s) 185
BstSCI CCNGG 1 cut(s) 602
BstSFI CTRYAG 6 cut(s) 243, 252, 272, 521, 1251, 1531
BstSLI GKGCMC 3 cut(s) 466, 734, 872
BstV2I GAAGAC 1 cut(s) 908
BstX2I RGATCY 4 cut(s) 599, 646, 844, 1473
BstXI CCANNNNNNTGG 2 cut(s) 741, 1191
BstYI RGATCY 4 cut(s) 599, 646, 844, 1473
Bsu36I CCTNAGG 1 cut(s) 536
BsuI GTATCC 1 cut(s) 1007
BsuRI GGCC 5 cut(s) 95, 345, 577, 745, 936
BtgZI GCGATG 2 cut(s) 78, 1086
BtrI CACGTC 1 cut(s) 559
BtsCI GGATG 4 cut(s) 222, 361, 576, 1211
BtsI GCAGTG 1 cut(s) 820
BtsIMutI CAGTG 6 cut(s) 466, 565, 585, 592, 683, 820
BveI ACCTGC 1 cut(s) 528
Cac8I GCNNGC 1 cut(s) 1051
CaiI CAGNNNCTG 1 cut(s) 251
CciI TCATGA 1 cut(s) 1559
CfoI GCGC 1 cut(s) 483
Cfr13I GGNCC 1 cut(s) 344
Csp6I GTAC 6 cut(s) 54, 118, 457, 850, 958, 1280
CviQI GTAC 6 cut(s) 54, 118, 457, 850, 958, 1280
DdeI CTNAG 9 cut(s) 9, 142, 174, 536, 626, 1449, 1491, 1507, 1513
DpnI GATC 7 cut(s) 340, 563, 601, 648, 846, 1475, 1519
DpnII GATC 7 cut(s) 338, 561, 599, 646, 844, 1473, 1517
DraI TTTAAA 1 cut(s) 490
EaeI YGGCCR 1 cut(s) 743
Eam1104I CTCTTC 1 cut(s) 1151
EarI CTCTTC 1 cut(s) 1151
Eco130I CCWWGG 1 cut(s) 1440
Eco31I GGTCTC 1 cut(s) 665
Eco57I CTGAAG 3 cut(s) 822, 920, 1398
Eco72I CACGTG 1 cut(s) 1191
Eco81I CCTNAGG 1 cut(s) 536
EcoNI CCTNNNNNAGG 1 cut(s) 539
EcoRI GAATTC 1 cut(s) 940
EcoRII CCWGG 1 cut(s) 602
EcoT14I CCWWGG 1 cut(s) 1440
ErhI CCWWGG 1 cut(s) 1440
Esp3I CGTCTC 1 cut(s) 5
FalI AAGNNNNNCTT 6 cut(s) 114, 146, 297, 329, 975, 1007
FauNDI CATATG 1 cut(s) 1526
FokI GGATG 4 cut(s) 209, 368, 583, 1218
FspBI CTAG 1 cut(s) 1298
GlaI GCGC 1 cut(s) 482
GsaI CCCAGC 1 cut(s) 875
GsuI CTGGAG 3 cut(s) 312, 418, 625
HaeII RGCGCY 1 cut(s) 484
HaeIII GGCC 5 cut(s) 95, 345, 577, 745, 936
HhaI GCGC 1 cut(s) 483
Hin6I GCGC 1 cut(s) 481
HinP1I GCGC 1 cut(s) 481
HphI GGTGA 1 cut(s) 371
Hpy166II GTNNAC 3 cut(s) 464, 852, 883
Hpy188I TCNGA 8 cut(s) 106, 231, 627, 900, 1079, 1480, 1494, 1545
Hpy8I GTNNAC 3 cut(s) 464, 852, 883
Hpy99I CGWCG 1 cut(s) 1394
HpyAV CCTTC 3 cut(s) 386, 477, 535
HpyCH4III ACNGT 5 cut(s) 117, 461, 687, 867, 1353
HpyCH4IV ACGT 3 cut(s) 160, 558, 1190
HpyF10VI GCNNNNNNNGC 8 cut(s) 86, 89, 98, 268, 280, 1079, 1088, 1262
HpyF3I CTNAG 9 cut(s) 9, 142, 174, 536, 626, 1449, 1491, 1507, 1513
HpySE526I ACGT 3 cut(s) 160, 558, 1190
HspAI GCGC 1 cut(s) 481
KpnI GGTACC 1 cut(s) 57
Kzo9I GATC 7 cut(s) 338, 561, 599, 646, 844, 1473, 1517
LmnI GCTCC 3 cut(s) 409, 445, 1362
LweI GCATC 1 cut(s) 1057
MaeI CTAG 1 cut(s) 1298
MaeII ACGT 3 cut(s) 160, 558, 1190
MaeIII GTNAC 4 cut(s) 164, 611, 652, 681
MalI GATC 7 cut(s) 340, 563, 601, 648, 846, 1475, 1519
MboI GATC 7 cut(s) 338, 561, 599, 646, 844, 1473, 1517
MboII GAAGA 7 cut(s) 185, 828, 913, 983, 985, 1138, 1391
MfeI CAATTG 1 cut(s) 75
MflI RGATCY 4 cut(s) 599, 646, 844, 1473
MhlI GDGCHC 3 cut(s) 466, 734, 872
MlsI TGGCCA 1 cut(s) 745
MluCI AATT 7 cut(s) 75, 198, 376, 794, 819, 940, 1218
MluNI TGGCCA 1 cut(s) 745
MlyI GAGTC 3 cut(s) 409, 898, 1404
MmeI TCCRAC 2 cut(s) 129, 530
MnlI CCTC 6 cut(s) 12, 273, 545, 588, 990, 1022
Mox20I TGGCCA 1 cut(s) 745
MscI TGGCCA 1 cut(s) 745
MseI TTAA 2 cut(s) 489, 1125
MslI CAYNNNNRTG 2 cut(s) 1189, 1467
Msp20I TGGCCA 1 cut(s) 745
MspA1I CMGCKG 1 cut(s) 426
MspR9I CCNGG 1 cut(s) 604
MunI CAATTG 1 cut(s) 75
Mva1269I GAATGC 1 cut(s) 753
MvaI CCWGG 1 cut(s) 604
MwoI GCNNNNNNNGC 8 cut(s) 86, 89, 98, 268, 280, 1079, 1088, 1262
NdeI CATATG 1 cut(s) 1526
NdeII GATC 7 cut(s) 338, 561, 599, 646, 844, 1473, 1517
NlaIV GGNNCC 5 cut(s) 55, 528, 601, 1344, 1364
NmuCI GTSAC 3 cut(s) 164, 611, 681
NspI RCATGY 1 cut(s) 185
PagI TCATGA 1 cut(s) 1559
PcsI WCGNNNNNNNCGW 1 cut(s) 20
PctI GAATGC 1 cut(s) 753
PfeI GAWTC 7 cut(s) 622, 976, 1004, 1094, 1269, 1445, 1556
PflFI GACNNNGTC 1 cut(s) 1394
PfoI TCCNGGA 1 cut(s) 602
PleI GAGTC 3 cut(s) 408, 898, 1403
PmaCI CACGTG 1 cut(s) 1191
PmlI CACGTG 1 cut(s) 1191
PpsI GAGTC 3 cut(s) 408, 898, 1403
Ppu21I YACGTR 1 cut(s) 1191
PshBI ATTAAT 1 cut(s) 1125
PsiI TTATAA 1 cut(s) 954
Psp6I CCWGG 1 cut(s) 602
PspCI CACGTG 1 cut(s) 1191
PspFI CCCAGC 1 cut(s) 871
PspGI CCWGG 1 cut(s) 602
PspN4I GGNNCC 5 cut(s) 55, 528, 601, 1344, 1364
PspPI GGNCC 1 cut(s) 344
PstI CTGCAG 3 cut(s) 276, 525, 1255
PstNI CAGNNNCTG 1 cut(s) 251
PsuI RGATCY 4 cut(s) 599, 646, 844, 1473
PsyI GACNNNGTC 1 cut(s) 1394
RsaI GTAC 6 cut(s) 55, 119, 458, 851, 959, 1281
RsaNI GTAC 6 cut(s) 54, 118, 457, 850, 958, 1280
RseI CAYNNNNRTG 2 cut(s) 1189, 1467
SaqAI TTAA 2 cut(s) 489, 1125
Sau3AI GATC 7 cut(s) 338, 561, 599, 646, 844, 1473, 1517
Sau96I GGNCC 1 cut(s) 344
SbfI CCTGCAGG 1 cut(s) 525
SchI GAGTC 3 cut(s) 409, 898, 1404
ScrFI CCNGG 1 cut(s) 604
SdaI CCTGCAGG 1 cut(s) 525
SduI GDGCHC 3 cut(s) 466, 734, 872
SfaNI GCATC 1 cut(s) 1057
SfcI CTRYAG 6 cut(s) 243, 252, 272, 521, 1251, 1531
SmiMI CAYNNNNRTG 2 cut(s) 1189, 1467
SmlI CTYRAG 2 cut(s) 981, 1013
SmoI CTYRAG 2 cut(s) 981, 1013
Sse8387I CCTGCAGG 1 cut(s) 525
Sse9I AATT 7 cut(s) 75, 198, 376, 794, 819, 940, 1218
SsiI CCGC 5 cut(s) 89, 92, 407, 424, 1211
SspI AATATT 1 cut(s) 893
SspMI CTAG 1 cut(s) 1298
StyD4I CCNGG 1 cut(s) 602
StyI CCWWGG 1 cut(s) 1440
TaaI ACNGT 5 cut(s) 117, 461, 687, 867, 1353
TaiI ACGT 3 cut(s) 163, 561, 1193
TaqI TCGA 2 cut(s) 749, 1466
TasI AATT 7 cut(s) 75, 198, 376, 794, 819, 940, 1218
TatI WGTACW 2 cut(s) 849, 957
TauI GCSGC 3 cut(s) 92, 95, 1214
TfiI GAWTC 7 cut(s) 622, 976, 1004, 1094, 1269, 1445, 1556
Tru1I TTAA 2 cut(s) 489, 1125
Tru9I TTAA 2 cut(s) 489, 1125
TscAI CASTG 6 cut(s) 466, 572, 592, 599, 690, 820
TseFI GTSAC 3 cut(s) 164, 611, 681
Tsp45I GTSAC 3 cut(s) 164, 611, 681
TspDTI ATGAA 8 cut(s) 31, 355, 459, 789, 801, 1086, 1211, 1548
TspGWI ACGGA 4 cut(s) 29, 407, 444, 1407
TspRI CASTG 6 cut(s) 466, 572, 592, 599, 690, 820
Tth111I GACNNNGTC 1 cut(s) 1394
VneI GTGCAC 1 cut(s) 462
VspI ATTAAT 1 cut(s) 1125
XagI CCTNNNNNAGG 1 cut(s) 539
XapI RAATTY 3 cut(s) 376, 819, 940
XceI RCATGY 1 cut(s) 185
XspI CTAG 1 cut(s) 1298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.