RchiOBHm_Chr7g0224451

Sterol 3-beta-glucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0014580
Physical Location & Seq
Forward (+)
46585778 .. 46594081
8304 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20098

Sequence Viewer

Length: 1551 bp
ATGGAGGTTGAGACGGAGAGGACGAAACCCGTTGCTGTATTCATGGCTTTCGGTACCAAAGGCGATGTCTATCCAATTGCTGCCATTGCGGCGGCCTTTGCTTCCGACCAGAGACAGTACCAAGTTGTTCTTATCACACATTCAGCTCACGAGAGCTTACGTTGTCACTTGGCTGAGAAGCATGTTTCCTATCTTCCAATTTCATCACCACCTGTTCTCTCTATCCATCAGAAACATGATACTACAGGCTCTGTAGAGTTGCCTCTTTCACTGCAGAAAAAGTTATTTTCTGGAGAACATAGAAAAGAATGTCTTTCGGTAGTTGAAACGATATTTGGATCAGGCCCAAGCATGGATGGTGATTTCATTGTGATAAATTTCTTTGCTCTGGAAGGCTGGAGTCTTGCGGAGCTTTTCCGTGTCCGCTGCATGGTTGCTGCTCCTTATGTTGTTCCGTACAGTGCACCTTCATCATTTGAGCGCCAGTTTAAAAAGGAATATCAACACTTGTATAGATACCTGCATGAACCTAACCTCAGGAAGGTTGGTTGGAAAGACGTGATCCACTGGATGTGGCCTCTTTTCACTGAACACTGGGGATCCTGGAGATGTGACCATTTGAATCTGAGTGCTTTCCCTTTTACAGATCCAGTAACGGGTCTCCCCACTTGGCATGATAGGCCACTGTCTCCCCGACTTTTGTATGGTTTTAGCAAAGAAGTTGTTGAGTGCCCAGATTACTGGCCATCGAGCATTCGGGTTTGTGGCTTTTGGTTTCTGCCTAATGAATGGCAATTTTCATGTAAGAAGTGCAGTGAAATTTCAAGGCTTGTTTCTTCAGGGGATCTGTACACAAATGTTGAAATGTGCCCAACTCATGTTCACTTGCAATATTTTCTGAAGACTCCTACGCCTATGCCACCTGTTTTTATTGGCCTGAATTCTATTGTACCCTTCATGGGATTTTTGAAGAATCCTCAAGCATTTCTTCGTATTCTTGAATCTGTCCTCAAGATAACAACTTATCGTTTTATCCTTTTTACTGCTGGCTACAAGCCTTTAGATGCAGCAATCCGAGCTATTGCTGCTGATTCATCATCGCACATGACCCACAAACAGATTAATGACGATTGCGTTTCTCTCTTCCACGAACGATTGTTCTGCTTTTCTGGTTCTATACCATACATGTGGCTGTTTTCTGGATGTGCGGCTGCAATTCATCATGGGGGAAGTGGAACTACTGCTGCAGCACTACTTGCAGGAATCCCACAGGTACTATGTCCATTTATGCTAGACCAGTTTTATTGGGCAGAAAGAATGTTTTGGCTTGGAGTGGCATCAGAACCGTTGAAAAGGAGCCACTTGCTTCCTGAAGAAAGTGATGACGAAACTATCGAAGAAGCAGCCAAGATGCTATCAAGAGTCATACATGATACATTATCTCCTGAAATTAGAGAACGCGCTGTAGAAATTTCTGAGAGAATATCTCTTGAGGACGGAGTCTCAAATGCTGTTAGGTATATCAAGGAAGAGATTGGTTGTTCAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

516

Amino Acids

58.6

Weight (kDa)

6.19

Isoelectric Point (pI)

41.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EryCIII-like_C PF06722 392 - 448 3.3e-12 Erythromycin biosynthesis protein CIII-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014580)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G24750
fragaria_vesca FvH4_5g30191 FvH4_5g30191
malus_domestica MD08G1224800.v1.1
prunus_persica Prupe.1G562100_v2.0.a1
pyrus_communis pycom08g19590
rosa_chinensis RchiOBHm_Chr7g0224451
rosa_laevigata RLG00000001894
rosa_multiflora Rmu_sc0003894.1_g000001
rosa_roxburghii Rroxscaffold_3G00234750
rosa_rugosa Rorug07G0223300 Rorug07G0223400
rosa_samantha Rh7AG379900 Rh7BG359500 Rh7CG399400 Rh7DG369100
rosa_wichuraiana Rw7G031180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 528
Acc65I GGTACC 1 cut(s) 53
AccB1I GGYRCC 1 cut(s) 53
AccII CGCG 1 cut(s) 1461
AciI CCGC 5 cut(s) 89, 92, 407, 424, 1208
AclWI GGATC 6 cut(s) 346, 556, 594, 607, 641, 852
AcoI YGGCCR 1 cut(s) 743
AcsI RAATTY 4 cut(s) 376, 819, 940, 1470
AcuI CTGAAG 3 cut(s) 822, 920, 1392
AfaI GTAC 6 cut(s) 55, 119, 458, 851, 951, 1275
AfiI CCNNNNNNNGG 5 cut(s) 352, 430, 541, 656, 959
AflIII ACRYGT 1 cut(s) 1185
AgsI TTSAA 8 cut(s) 326, 622, 825, 863, 970, 1001, 1351, 1545
AjiI CACGTC 1 cut(s) 559
AjnI CCWGG 1 cut(s) 602
AjuI GAANNNNNNNTTGG 2 cut(s) 318, 350
AluBI AGCT 5 cut(s) 146, 156, 412, 1079, 1548
AluI AGCT 5 cut(s) 146, 156, 412, 1079, 1548
Alw21I GWGCWC 1 cut(s) 466
Alw26I GTCTC 5 cut(s) 5, 106, 665, 693, 1507
Alw44I GTGCAC 1 cut(s) 462
AlwI GGATC 6 cut(s) 346, 556, 594, 607, 641, 852
AlwNI CAGNNNCTG 1 cut(s) 251
AoxI GGCC 6 cut(s) 93, 343, 575, 680, 743, 934
ApaLI GTGCAC 1 cut(s) 462
ApeKI GCWGC 9 cut(s) 80, 426, 437, 1067, 1085, 1211, 1244, 1247, 1403
ApoI RAATTY 4 cut(s) 376, 819, 940, 1470
ArsI GACNNNNNNTTYG 2 cut(s) 51, 83
AseI ATTAAT 1 cut(s) 1122
Asp718I GGTACC 1 cut(s) 53
AspLEI GCGC 2 cut(s) 483, 1463
AspS9I GGNCC 1 cut(s) 344
AsuHPI GGTGA 2 cut(s) 198, 371
AxyI CCTNAGG 1 cut(s) 536
BaeGI GKGCMC 3 cut(s) 466, 734, 872
BalI TGGCCA 1 cut(s) 745
BamHI GGATCC 1 cut(s) 599
BanI GGYRCC 1 cut(s) 53
BarI GAAGNNNNNNTAC 2 cut(s) 1222, 1254
BauI CACGAG 1 cut(s) 149
BbsI GAAGAC 1 cut(s) 908
Bbv12I GWGCWC 1 cut(s) 466
BbvI GCAGC 9 cut(s) 67, 413, 424, 1072, 1079, 1198, 1231, 1259, 1415
BccI CCATC 3 cut(s) 234, 350, 754
BcgI CGANNNNNNTGC 2 cut(s) 1143, 1177
BciT130I CCWGG 1 cut(s) 604
BcoDI GTCTC 5 cut(s) 5, 106, 665, 693, 1507
BfaI CTAG 1 cut(s) 1292
BfmI CTRYAG 5 cut(s) 243, 252, 272, 1245, 1464
BfoI RGCGCY 1 cut(s) 484
BfuAI ACCTGC 1 cut(s) 528
BglI GCCNNNNNGGC 1 cut(s) 89
Bme1390I CCNGG 1 cut(s) 604
BmgBI CACGTC 1 cut(s) 559
BmgT120I GGNCC 1 cut(s) 344
BmiI GGNNCC 3 cut(s) 55, 601, 1358
BmrFI CCNGG 1 cut(s) 604
BmrI ACTGGG 1 cut(s) 604
BmsI GCATC 3 cut(s) 1054, 1346, 1401
BmuI ACTGGG 1 cut(s) 604
BpiI GAAGAC 1 cut(s) 908
BplI GAGNNNNNCTC 2 cut(s) 1471, 1503
BpmI CTGGAG 3 cut(s) 312, 418, 625
BpuEI CTTGAG 3 cut(s) 963, 995, 1511
BsaBI GATNNNNATC 1 cut(s) 69
BsaI GGTCTC 1 cut(s) 665
BsaXI ACNNNNNCTCC 2 cut(s) 1426, 1456
Bsc4I CCNNNNNNNGG 5 cut(s) 352, 430, 541, 656, 959
Bse1I ACTGG 6 cut(s) 484, 572, 599, 650, 746, 1297
Bse21I CCTNAGG 1 cut(s) 536
Bse3DI GCAATG 1 cut(s) 84
Bse8I GATNNNNATC 1 cut(s) 69
BseBI CCWGG 1 cut(s) 604
BseGI GGATG 3 cut(s) 361, 576, 1208
BseJI GATNNNNATC 1 cut(s) 69
BseLI CCNNNNNNNGG 5 cut(s) 352, 430, 541, 656, 959
BseMI GCAATG 1 cut(s) 84
BseMII CTCAG 4 cut(s) 165, 550, 617, 1467
BseNI ACTGG 6 cut(s) 484, 572, 599, 650, 746, 1297
BseSI GKGCMC 3 cut(s) 466, 734, 872
BseXI GCAGC 9 cut(s) 67, 413, 424, 1072, 1079, 1198, 1231, 1259, 1415
BsgI GTGCAG 1 cut(s) 832
Bsh1236I CGCG 1 cut(s) 1461
BshFI GGCC 6 cut(s) 95, 345, 577, 682, 745, 936
BshNI GGYRCC 1 cut(s) 53
BsiHKAI GWGCWC 1 cut(s) 466
BslI CCNNNNNNNGG 5 cut(s) 352, 430, 541, 656, 959
BsmAI GTCTC 5 cut(s) 5, 106, 665, 693, 1507
BsmBI CGTCTC 1 cut(s) 5
BsmI GAATGC 1 cut(s) 753
BsnI GGCC 6 cut(s) 95, 345, 577, 682, 745, 936
Bso31I GGTCTC 1 cut(s) 665
Bsp1286I GDGCHC 3 cut(s) 466, 734, 872
Bsp1407I TGTACA 1 cut(s) 849
Bsp143I GATC 5 cut(s) 338, 561, 599, 646, 844
BspACI CCGC 5 cut(s) 89, 92, 407, 424, 1208
BspANI GGCC 6 cut(s) 95, 345, 577, 682, 745, 936
BspCNI CTCAG 4 cut(s) 166, 549, 618, 1468
BspFNI CGCG 1 cut(s) 1461
BspLI GGNNCC 3 cut(s) 55, 601, 1358
BspMAI CTGCAG 2 cut(s) 276, 1249
BspMI ACCTGC 1 cut(s) 528
BspPI GGATC 6 cut(s) 346, 556, 594, 607, 641, 852
BspT107I GGYRCC 1 cut(s) 53
BspTNI GGTCTC 1 cut(s) 665
BsrDI GCAATG 1 cut(s) 84
BsrGI TGTACA 1 cut(s) 849
BsrI ACTGG 6 cut(s) 484, 572, 599, 650, 746, 1297
BssMI GATC 5 cut(s) 338, 561, 599, 646, 844
BssSI CACGAG 1 cut(s) 149
Bst2BI CACGAG 1 cut(s) 149
Bst2UI CCWGG 1 cut(s) 604
Bst4CI ACNGT 4 cut(s) 117, 461, 687, 1347
Bst6I CTCTTC 2 cut(s) 1148, 1524
BstAPI GCANNNNNTGC 1 cut(s) 1256
BstAUI TGTACA 1 cut(s) 849
BstC8I GCNNGC 1 cut(s) 1048
BstDEI CTNAG 4 cut(s) 174, 536, 626, 1476
BstENI CCTNNNNNAGG 1 cut(s) 539
BstF5I GGATG 3 cut(s) 361, 576, 1208
BstFNI CGCG 1 cut(s) 1461
BstH2I RGCGCY 1 cut(s) 484
BstHHI GCGC 2 cut(s) 483, 1463
BstKTI GATC 5 cut(s) 341, 564, 602, 649, 847
BstMAI GTCTC 5 cut(s) 5, 106, 665, 693, 1507
BstMBI GATC 5 cut(s) 338, 561, 599, 646, 844
BstMWI GCNNNNNNNGC 7 cut(s) 86, 89, 98, 679, 1076, 1085, 1256
BstNI CCWGG 1 cut(s) 604
BstNSI RCATGY 2 cut(s) 185, 1189
BstSCI CCNGG 1 cut(s) 602
BstSFI CTRYAG 5 cut(s) 243, 252, 272, 1245, 1464
BstSLI GKGCMC 3 cut(s) 466, 734, 872
BstUI CGCG 1 cut(s) 1461
BstV1I GCAGC 9 cut(s) 67, 413, 424, 1072, 1079, 1198, 1231, 1259, 1415
BstV2I GAAGAC 1 cut(s) 908
BstX2I RGATCY 3 cut(s) 599, 646, 844
BstXI CCANNNNNNTGG 2 cut(s) 741, 1188
BstYI RGATCY 3 cut(s) 599, 646, 844
Bsu36I CCTNAGG 1 cut(s) 536
BsuRI GGCC 6 cut(s) 95, 345, 577, 682, 745, 936
BtgZI GCGATG 2 cut(s) 78, 1083
BtrI CACGTC 1 cut(s) 559
BtsCI GGATG 3 cut(s) 361, 576, 1208
BtsI GCAGTG 2 cut(s) 269, 820
BtsIMutI CAGTG 7 cut(s) 269, 466, 565, 585, 592, 683, 820
BveI ACCTGC 1 cut(s) 528
Cac8I GCNNGC 1 cut(s) 1048
CaiI CAGNNNCTG 1 cut(s) 251
CfoI GCGC 2 cut(s) 483, 1463
Cfr13I GGNCC 1 cut(s) 344
Csp6I GTAC 6 cut(s) 54, 118, 457, 850, 950, 1274
CviQI GTAC 6 cut(s) 54, 118, 457, 850, 950, 1274
DdeI CTNAG 4 cut(s) 174, 536, 626, 1476
DpnI GATC 5 cut(s) 340, 563, 601, 648, 846
DpnII GATC 5 cut(s) 338, 561, 599, 646, 844
DraI TTTAAA 1 cut(s) 490
EaeI YGGCCR 1 cut(s) 743
Eam1104I CTCTTC 2 cut(s) 1148, 1524
EarI CTCTTC 2 cut(s) 1148, 1524
Eco31I GGTCTC 1 cut(s) 665
Eco57I CTGAAG 3 cut(s) 822, 920, 1392
Eco81I CCTNAGG 1 cut(s) 536
EcoNI CCTNNNNNAGG 1 cut(s) 539
EcoRI GAATTC 1 cut(s) 940
EcoRII CCWGG 1 cut(s) 602
Esp3I CGTCTC 1 cut(s) 5
FalI AAGNNNNNCTT 6 cut(s) 114, 146, 297, 329, 972, 1004
FokI GGATG 3 cut(s) 368, 583, 1215
FspBI CTAG 1 cut(s) 1292
GlaI GCGC 2 cut(s) 482, 1462
GsuI CTGGAG 3 cut(s) 312, 418, 625
HaeII RGCGCY 1 cut(s) 484
HaeIII GGCC 6 cut(s) 95, 345, 577, 682, 745, 936
HhaI GCGC 2 cut(s) 483, 1463
Hin6I GCGC 2 cut(s) 481, 1461
HinP1I GCGC 2 cut(s) 481, 1461
HinfI GANTC 9 cut(s) 400, 622, 904, 973, 1001, 1091, 1263, 1422, 1500
HphI GGTGA 2 cut(s) 198, 371
Hpy166II GTNNAC 3 cut(s) 464, 852, 883
Hpy188I TCNGA 7 cut(s) 106, 231, 627, 900, 1076, 1342, 1477
Hpy8I GTNNAC 3 cut(s) 464, 852, 883
HpyAV CCTTC 4 cut(s) 386, 477, 535, 964
HpyCH4III ACNGT 4 cut(s) 117, 461, 687, 1347
HpyCH4IV ACGT 2 cut(s) 160, 558
HpyF10VI GCNNNNNNNGC 7 cut(s) 86, 89, 98, 679, 1076, 1085, 1256
HpyF3I CTNAG 4 cut(s) 174, 536, 626, 1476
HpySE526I ACGT 2 cut(s) 160, 558
HspAI GCGC 2 cut(s) 481, 1461
KpnI GGTACC 1 cut(s) 57
Kzo9I GATC 5 cut(s) 338, 561, 599, 646, 844
LmnI GCTCC 3 cut(s) 409, 445, 1356
Lsp1109I GCAGC 9 cut(s) 67, 413, 424, 1072, 1079, 1198, 1231, 1259, 1415
LweI GCATC 3 cut(s) 1054, 1346, 1401
MaeI CTAG 1 cut(s) 1292
MaeII ACGT 2 cut(s) 160, 558
MaeIII GTNAC 3 cut(s) 164, 611, 652
MalI GATC 5 cut(s) 340, 563, 601, 648, 846
MboI GATC 5 cut(s) 338, 561, 599, 646, 844
MboII GAAGA 9 cut(s) 185, 828, 913, 980, 982, 1135, 1385, 1409, 1541
MfeI CAATTG 1 cut(s) 75
MflI RGATCY 3 cut(s) 599, 646, 844
MhlI GDGCHC 3 cut(s) 466, 734, 872
MlsI TGGCCA 1 cut(s) 745
MluCI AATT 9 cut(s) 75, 198, 376, 794, 819, 940, 1215, 1449, 1470
MluNI TGGCCA 1 cut(s) 745
MlyI GAGTC 4 cut(s) 409, 898, 1431, 1509
MmeI TCCRAC 2 cut(s) 129, 530
MnlI CCTC 7 cut(s) 12, 273, 545, 588, 987, 1019, 1486
Mox20I TGGCCA 1 cut(s) 745
MscI TGGCCA 1 cut(s) 745
MseI TTAA 2 cut(s) 489, 1122
MslI CAYNNNNRTG 1 cut(s) 1186
Msp20I TGGCCA 1 cut(s) 745
MspA1I CMGCKG 1 cut(s) 426
MspR9I CCNGG 1 cut(s) 604
MunI CAATTG 1 cut(s) 75
Mva1269I GAATGC 1 cut(s) 753
MvaI CCWGG 1 cut(s) 604
MvnI CGCG 1 cut(s) 1461
MwoI GCNNNNNNNGC 7 cut(s) 86, 89, 98, 679, 1076, 1085, 1256
NdeII GATC 5 cut(s) 338, 561, 599, 646, 844
NlaIV GGNNCC 3 cut(s) 55, 601, 1358
NmuCI GTSAC 2 cut(s) 164, 611
NspI RCATGY 2 cut(s) 185, 1189
PciI ACATGT 1 cut(s) 1185
PcsI WCGNNNNNNNCGW 2 cut(s) 20, 1392
PctI GAATGC 1 cut(s) 753
PfeI GAWTC 5 cut(s) 622, 973, 1001, 1091, 1263
PflFI GACNNNGTC 1 cut(s) 1499
PfoI TCCNGGA 1 cut(s) 602
PleI GAGTC 4 cut(s) 408, 898, 1430, 1508
PpsI GAGTC 4 cut(s) 408, 898, 1430, 1508
PscI ACATGT 1 cut(s) 1185
PshBI ATTAAT 1 cut(s) 1122
Psp6I CCWGG 1 cut(s) 602
PspGI CCWGG 1 cut(s) 602
PspN4I GGNNCC 3 cut(s) 55, 601, 1358
PspPI GGNCC 1 cut(s) 344
PstI CTGCAG 2 cut(s) 276, 1249
PstNI CAGNNNCTG 1 cut(s) 251
PsuI RGATCY 3 cut(s) 599, 646, 844
PsyI GACNNNGTC 1 cut(s) 1499
RsaI GTAC 6 cut(s) 55, 119, 458, 851, 951, 1275
RsaNI GTAC 6 cut(s) 54, 118, 457, 850, 950, 1274
RseI CAYNNNNRTG 1 cut(s) 1186
SaqAI TTAA 2 cut(s) 489, 1122
Sau3AI GATC 5 cut(s) 338, 561, 599, 646, 844
Sau96I GGNCC 1 cut(s) 344
SchI GAGTC 4 cut(s) 409, 898, 1431, 1509
ScrFI CCNGG 1 cut(s) 604
SduI GDGCHC 3 cut(s) 466, 734, 872
SfaNI GCATC 3 cut(s) 1054, 1346, 1401
SfcI CTRYAG 5 cut(s) 243, 252, 272, 1245, 1464
SmiMI CAYNNNNRTG 1 cut(s) 1186
SmlI CTYRAG 3 cut(s) 978, 1010, 1490
SmoI CTYRAG 3 cut(s) 978, 1010, 1490
Sse9I AATT 9 cut(s) 75, 198, 376, 794, 819, 940, 1215, 1449, 1470
SsiI CCGC 5 cut(s) 89, 92, 407, 424, 1208
SspI AATATT 1 cut(s) 893
SspMI CTAG 1 cut(s) 1292
StyD4I CCNGG 1 cut(s) 602
TaaI ACNGT 4 cut(s) 117, 461, 687, 1347
TaiI ACGT 2 cut(s) 163, 561
TaqI TCGA 2 cut(s) 749, 1395
TasI AATT 9 cut(s) 75, 198, 376, 794, 819, 940, 1215, 1449, 1470
TatI WGTACW 1 cut(s) 849
TauI GCSGC 3 cut(s) 92, 95, 1211
TfiI GAWTC 5 cut(s) 622, 973, 1001, 1091, 1263
Tru1I TTAA 2 cut(s) 489, 1122
Tru9I TTAA 2 cut(s) 489, 1122
TscAI CASTG 7 cut(s) 276, 466, 572, 592, 599, 690, 820
TseFI GTSAC 2 cut(s) 164, 611
TseI GCWGC 9 cut(s) 80, 426, 437, 1067, 1085, 1211, 1244, 1247, 1403
Tsp45I GTSAC 2 cut(s) 164, 611
TspGWI ACGGA 4 cut(s) 29, 407, 444, 1512
TspRI CASTG 7 cut(s) 276, 466, 572, 592, 599, 690, 820
Tth111I GACNNNGTC 1 cut(s) 1499
VneI GTGCAC 1 cut(s) 462
VspI ATTAAT 1 cut(s) 1122
XagI CCTNNNNNAGG 1 cut(s) 539
XapI RAATTY 4 cut(s) 376, 819, 940, 1470
XceI RCATGY 2 cut(s) 185, 1189
XspI CTAG 1 cut(s) 1292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.