Rh7AG379900

Sterol 3-beta-glucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0014580
Physical Location & Seq
Reverse (-)
49591504 .. 49598188
6685 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG379900.1

Sequence Viewer

Length: 1401 bp
ATGCAGAGCTTACGTTGTCACTTGGCTGAGAAGCATGTTTCCTATCTTCCAATTTCATCACCACCTGTTCTCTCTATCCATCAGAAACATGATACTACAGGCTCTGTAGAGTTGCCTCTTTCACTGCAGAAAAAGTTGTTTTCTGGAGAACATAGAAAAGAATGTCTTTCGGTAGTTGAAACGATATTTGGATCAGGCCCAAGCATGGATGGTGATTTCATTGTGATAAATTTCTTTGCTCTGGAAGGCTGGAGTCTTGCGGAGCTTTTCCGTGTCCGCTGCATGGTTGCTGCTCCTTATGTTGTTCCGTACAGTGCACCTTCATCATTTGAGCGCCAGTTTAAAAAGGAATATCAACACTTGTATAGATACCTGCATGAACCTAACCTCAGGAAGGTTGGTTGGAAAGACGTGATCCACTGGATGTGGCCTCTTTTCACTGAACACTGGGGATCCTGGAGATGTGACCATTTGAATCTGAGTGCTTTCCCTTTTACAGATCCAGTAACGGGTCTCCCCACTTGGCATGATAGGCCACTGTCTCCCCGACTTTTGTATGGTTTTAGCAAAGAAGTTGTTGAGTGCCCAGATTACTGGCCATCGAGCATTCGGGTTTGTGGCTTTTGGTTTCTGCCTAATGAATGGCAATTTTCATGTAAGAAGTGCAGTGAAATCTCAAGGCTTGTTTCTTCAGGGGATCTGTACACAAATGCTGAAATGTGCCCAACTCATGTTCACTTGCAATATTTTCTGAAGACTCCTACGCCTATGCCACCTGTTTTTATTGGCCTGAATTCTATTGGTAGCATGGGATTTTTGAAGAATCCTCAAGCATTTCTTCGTATTCTTGAATCTGTCCTCAAGATAACAACTTATCGTTTTATCCTTTTTACTGCTGGCTACAAGCCTTTAGATGCAGCAATCCGAGCTATTGCTGCTGATTCATCATCGCACATGACCCACAAACAGATTAATGACGATTGCGTTTCTCTCTTCCACGAACGATTGTTCGGCTTTTCTGGTTCTATACCATACATGTGGTTGTTTTCTGGATGTGCGGCTGCAATTCATCATGGGGGAAGTGGAACTACTGCTGCAGCACTACTTGCAGGAATCCCACAGGTACTATGTCCATTTATGCTAGACCAGTTTTATTGGGCAGAAAGAATGTTTTGGCTTGGAGTGGCATCAGAACCGTTGAAAAGGAGCCACTTGCTTCCTGAAGAAAGTGATGACGAAAGTATCGAAGAAGCAGCCAAGATGCTATCAAGAGTCATACATGATACATTATCTCCTGAAATTAGAGAACGCGCTGTAGAAATTTCTGAGAGAATATCTCTTGAGGACGGAGTCTCAAATGCTGTTAGGTATATCAAGGAAGAGATTGGTTGTTCAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

466

Amino Acids

52.99

Weight (kDa)

6.28

Isoelectric Point (pI)

44.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EryCIII-like_C PF06722 342 - 398 2.3e-12 Erythromycin biosynthesis protein CIII-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014580)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G24750
fragaria_vesca FvH4_5g30191 FvH4_5g30191
malus_domestica MD08G1224800.v1.1
prunus_persica Prupe.1G562100_v2.0.a1
pyrus_communis pycom08g19590
rosa_chinensis RchiOBHm_Chr7g0224451
rosa_laevigata RLG00000001894
rosa_multiflora Rmu_sc0003894.1_g000001
rosa_roxburghii Rroxscaffold_3G00234750
rosa_rugosa Rorug07G0223300 Rorug07G0223400
rosa_samantha Rh7AG379900 Rh7BG359500 Rh7CG399400 Rh7DG369100
rosa_wichuraiana Rw7G031180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 381
AccII CGCG 1 cut(s) 1311
AciI CCGC 3 cut(s) 260, 277, 1058
AclWI GGATC 6 cut(s) 199, 409, 447, 460, 494, 705
AcoI YGGCCR 1 cut(s) 596
AcsI RAATTY 3 cut(s) 229, 793, 1320
AcuI CTGAAG 3 cut(s) 675, 773, 1242
AfaI GTAC 3 cut(s) 311, 704, 1125
AfiI CCNNNNNNNGG 4 cut(s) 205, 283, 394, 509
AflIII ACRYGT 1 cut(s) 1035
AgsI TTSAA 6 cut(s) 179, 475, 820, 851, 1201, 1395
AjiI CACGTC 1 cut(s) 412
AjnI CCWGG 1 cut(s) 455
AjuI GAANNNNNNNTTGG 2 cut(s) 171, 203
AluBI AGCT 4 cut(s) 9, 265, 929, 1398
AluI AGCT 4 cut(s) 9, 265, 929, 1398
Alw21I GWGCWC 1 cut(s) 319
Alw26I GTCTC 3 cut(s) 518, 546, 1357
Alw44I GTGCAC 1 cut(s) 315
AlwI GGATC 6 cut(s) 199, 409, 447, 460, 494, 705
AlwNI CAGNNNCTG 1 cut(s) 104
AoxI GGCC 5 cut(s) 196, 428, 533, 596, 787
ApaLI GTGCAC 1 cut(s) 315
ApeKI GCWGC 8 cut(s) 279, 290, 917, 935, 1061, 1094, 1097, 1253
ApoI RAATTY 3 cut(s) 229, 793, 1320
AseI ATTAAT 1 cut(s) 972
AspLEI GCGC 2 cut(s) 336, 1313
AspS9I GGNCC 1 cut(s) 197
AsuHPI GGTGA 2 cut(s) 51, 224
AxyI CCTNAGG 1 cut(s) 389
BaeGI GKGCMC 3 cut(s) 319, 587, 725
BaeI ACNNNNGTAYC 2 cut(s) 1225, 1258
BalI TGGCCA 1 cut(s) 598
BamHI GGATCC 1 cut(s) 452
BarI GAAGNNNNNNTAC 2 cut(s) 1072, 1104
BbsI GAAGAC 1 cut(s) 761
Bbv12I GWGCWC 1 cut(s) 319
BbvI GCAGC 8 cut(s) 266, 277, 922, 929, 1048, 1081, 1109, 1265
BccI CCATC 3 cut(s) 87, 203, 607
BciT130I CCWGG 1 cut(s) 457
BcoDI GTCTC 3 cut(s) 518, 546, 1357
BfaI CTAG 1 cut(s) 1142
BfmI CTRYAG 5 cut(s) 96, 105, 125, 1095, 1314
BfoI RGCGCY 1 cut(s) 337
BfuAI ACCTGC 1 cut(s) 381
BisI GCNGC 9 cut(s) 280, 291, 918, 936, 1059, 1062, 1095, 1098, 1254
BlsI GCNGC 9 cut(s) 281, 292, 919, 937, 1060, 1063, 1096, 1099, 1255
Bme1390I CCNGG 1 cut(s) 457
BmgBI CACGTC 1 cut(s) 412
BmgT120I GGNCC 1 cut(s) 197
BmiI GGNNCC 2 cut(s) 454, 1208
BmrFI CCNGG 1 cut(s) 457
BmrI ACTGGG 1 cut(s) 457
BmsI GCATC 3 cut(s) 904, 1196, 1251
BmuI ACTGGG 1 cut(s) 457
BpiI GAAGAC 1 cut(s) 761
BplI GAGNNNNNCTC 2 cut(s) 1321, 1353
BpmI CTGGAG 3 cut(s) 165, 271, 478
BpuEI CTTGAG 4 cut(s) 661, 813, 845, 1361
BsaI GGTCTC 1 cut(s) 518
BsaXI ACNNNNNCTCC 2 cut(s) 1276, 1306
Bsc4I CCNNNNNNNGG 4 cut(s) 205, 283, 394, 509
Bse1I ACTGG 6 cut(s) 337, 425, 452, 503, 599, 1147
Bse21I CCTNAGG 1 cut(s) 389
BseBI CCWGG 1 cut(s) 457
BseGI GGATG 3 cut(s) 214, 429, 1058
BseLI CCNNNNNNNGG 4 cut(s) 205, 283, 394, 509
BseMII CTCAG 4 cut(s) 18, 403, 470, 1317
BseNI ACTGG 6 cut(s) 337, 425, 452, 503, 599, 1147
BseSI GKGCMC 3 cut(s) 319, 587, 725
BseXI GCAGC 8 cut(s) 266, 277, 922, 929, 1048, 1081, 1109, 1265
BsgI GTGCAG 1 cut(s) 685
Bsh1236I CGCG 1 cut(s) 1311
BshFI GGCC 5 cut(s) 198, 430, 535, 598, 789
BsiHKAI GWGCWC 1 cut(s) 319
BslI CCNNNNNNNGG 4 cut(s) 205, 283, 394, 509
BsmAI GTCTC 3 cut(s) 518, 546, 1357
BsmI GAATGC 1 cut(s) 606
BsnI GGCC 5 cut(s) 198, 430, 535, 598, 789
Bso31I GGTCTC 1 cut(s) 518
Bsp1286I GDGCHC 3 cut(s) 319, 587, 725
Bsp1407I TGTACA 1 cut(s) 702
Bsp143I GATC 5 cut(s) 191, 414, 452, 499, 697
BspACI CCGC 3 cut(s) 260, 277, 1058
BspANI GGCC 5 cut(s) 198, 430, 535, 598, 789
BspCNI CTCAG 4 cut(s) 19, 402, 471, 1318
BspFNI CGCG 1 cut(s) 1311
BspLI GGNNCC 2 cut(s) 454, 1208
BspMAI CTGCAG 2 cut(s) 129, 1099
BspMI ACCTGC 1 cut(s) 381
BspPI GGATC 6 cut(s) 199, 409, 447, 460, 494, 705
BspTNI GGTCTC 1 cut(s) 518
BsrGI TGTACA 1 cut(s) 702
BsrI ACTGG 6 cut(s) 337, 425, 452, 503, 599, 1147
BssMI GATC 5 cut(s) 191, 414, 452, 499, 697
Bst2UI CCWGG 1 cut(s) 457
Bst4CI ACNGT 3 cut(s) 314, 540, 1197
Bst6I CTCTTC 2 cut(s) 998, 1374
BstAPI GCANNNNNTGC 1 cut(s) 1106
BstAUI TGTACA 1 cut(s) 702
BstC8I GCNNGC 1 cut(s) 898
BstDEI CTNAG 4 cut(s) 27, 389, 479, 1326
BstENI CCTNNNNNAGG 1 cut(s) 392
BstF5I GGATG 3 cut(s) 214, 429, 1058
BstFNI CGCG 1 cut(s) 1311
BstH2I RGCGCY 1 cut(s) 337
BstHHI GCGC 2 cut(s) 336, 1313
BstKTI GATC 5 cut(s) 194, 417, 455, 502, 700
BstMAI GTCTC 3 cut(s) 518, 546, 1357
BstMBI GATC 5 cut(s) 191, 414, 452, 499, 697
BstMWI GCNNNNNNNGC 4 cut(s) 532, 926, 935, 1106
BstNI CCWGG 1 cut(s) 457
BstNSI RCATGY 2 cut(s) 38, 1039
BstSCI CCNGG 1 cut(s) 455
BstSFI CTRYAG 5 cut(s) 96, 105, 125, 1095, 1314
BstSLI GKGCMC 3 cut(s) 319, 587, 725
BstUI CGCG 1 cut(s) 1311
BstV1I GCAGC 8 cut(s) 266, 277, 922, 929, 1048, 1081, 1109, 1265
BstV2I GAAGAC 1 cut(s) 761
BstX2I RGATCY 3 cut(s) 452, 499, 697
BstXI CCANNNNNNTGG 2 cut(s) 594, 1038
BstYI RGATCY 3 cut(s) 452, 499, 697
Bsu36I CCTNAGG 1 cut(s) 389
BsuRI GGCC 5 cut(s) 198, 430, 535, 598, 789
BtgZI GCGATG 1 cut(s) 933
BtrI CACGTC 1 cut(s) 412
BtsCI GGATG 3 cut(s) 214, 429, 1058
BtsI GCAGTG 2 cut(s) 122, 673
BtsIMutI CAGTG 7 cut(s) 122, 319, 418, 438, 445, 536, 673
BveI ACCTGC 1 cut(s) 381
Cac8I GCNNGC 1 cut(s) 898
CaiI CAGNNNCTG 1 cut(s) 104
CfoI GCGC 2 cut(s) 336, 1313
Cfr13I GGNCC 1 cut(s) 197
Csp6I GTAC 3 cut(s) 310, 703, 1124
CviQI GTAC 3 cut(s) 310, 703, 1124
DdeI CTNAG 4 cut(s) 27, 389, 479, 1326
DpnI GATC 5 cut(s) 193, 416, 454, 501, 699
DpnII GATC 5 cut(s) 191, 414, 452, 499, 697
DraI TTTAAA 1 cut(s) 343
EaeI YGGCCR 1 cut(s) 596
Eam1104I CTCTTC 2 cut(s) 998, 1374
EarI CTCTTC 2 cut(s) 998, 1374
Eco31I GGTCTC 1 cut(s) 518
Eco57I CTGAAG 3 cut(s) 675, 773, 1242
Eco81I CCTNAGG 1 cut(s) 389
EcoNI CCTNNNNNAGG 1 cut(s) 392
EcoRI GAATTC 1 cut(s) 793
EcoRII CCWGG 1 cut(s) 455
FalI AAGNNNNNCTT 4 cut(s) 150, 182, 822, 854
Fnu4HI GCNGC 9 cut(s) 280, 291, 918, 936, 1059, 1062, 1095, 1098, 1254
FokI GGATG 3 cut(s) 221, 436, 1065
Fsp4HI GCNGC 9 cut(s) 280, 291, 918, 936, 1059, 1062, 1095, 1098, 1254
FspBI CTAG 1 cut(s) 1142
GlaI GCGC 2 cut(s) 335, 1312
GluI GCNGC 9 cut(s) 280, 291, 918, 936, 1059, 1062, 1095, 1098, 1254
GsuI CTGGAG 3 cut(s) 165, 271, 478
HaeII RGCGCY 1 cut(s) 337
HaeIII GGCC 5 cut(s) 198, 430, 535, 598, 789
HhaI GCGC 2 cut(s) 336, 1313
Hin6I GCGC 2 cut(s) 334, 1311
HinP1I GCGC 2 cut(s) 334, 1311
HinfI GANTC 9 cut(s) 253, 475, 757, 823, 851, 941, 1113, 1272, 1350
HphI GGTGA 2 cut(s) 51, 224
Hpy166II GTNNAC 3 cut(s) 317, 705, 736
Hpy188I TCNGA 6 cut(s) 84, 480, 753, 926, 1192, 1327
Hpy8I GTNNAC 3 cut(s) 317, 705, 736
HpyAV CCTTC 3 cut(s) 239, 330, 388
HpyCH4III ACNGT 3 cut(s) 314, 540, 1197
HpyCH4IV ACGT 2 cut(s) 13, 411
HpyF10VI GCNNNNNNNGC 4 cut(s) 532, 926, 935, 1106
HpyF3I CTNAG 4 cut(s) 27, 389, 479, 1326
HpySE526I ACGT 2 cut(s) 13, 411
HspAI GCGC 2 cut(s) 334, 1311
Kzo9I GATC 5 cut(s) 191, 414, 452, 499, 697
LmnI GCTCC 3 cut(s) 262, 298, 1206
Lsp1109I GCAGC 8 cut(s) 266, 277, 922, 929, 1048, 1081, 1109, 1265
LweI GCATC 3 cut(s) 904, 1196, 1251
MaeI CTAG 1 cut(s) 1142
MaeII ACGT 2 cut(s) 13, 411
MaeIII GTNAC 3 cut(s) 17, 464, 505
MalI GATC 5 cut(s) 193, 416, 454, 501, 699
MboI GATC 5 cut(s) 191, 414, 452, 499, 697
MboII GAAGA 9 cut(s) 38, 681, 766, 830, 832, 985, 1235, 1259, 1391
MflI RGATCY 3 cut(s) 452, 499, 697
MhlI GDGCHC 3 cut(s) 319, 587, 725
MlsI TGGCCA 1 cut(s) 598
MluCI AATT 7 cut(s) 51, 229, 647, 793, 1065, 1299, 1320
MluNI TGGCCA 1 cut(s) 598
MlyI GAGTC 4 cut(s) 262, 751, 1281, 1359
MmeI TCCRAC 1 cut(s) 383
MnlI CCTC 6 cut(s) 126, 398, 441, 837, 869, 1336
Mox20I TGGCCA 1 cut(s) 598
MscI TGGCCA 1 cut(s) 598
MseI TTAA 2 cut(s) 342, 972
MslI CAYNNNNRTG 1 cut(s) 1036
Msp20I TGGCCA 1 cut(s) 598
MspA1I CMGCKG 1 cut(s) 279
MspR9I CCNGG 1 cut(s) 457
Mva1269I GAATGC 1 cut(s) 606
MvaI CCWGG 1 cut(s) 457
MvnI CGCG 1 cut(s) 1311
MwoI GCNNNNNNNGC 4 cut(s) 532, 926, 935, 1106
NdeII GATC 5 cut(s) 191, 414, 452, 499, 697
NlaIV GGNNCC 2 cut(s) 454, 1208
NmuCI GTSAC 2 cut(s) 17, 464
NspI RCATGY 2 cut(s) 38, 1039
PciI ACATGT 1 cut(s) 1035
PcsI WCGNNNNNNNCGW 1 cut(s) 1242
PctI GAATGC 1 cut(s) 606
PfeI GAWTC 5 cut(s) 475, 823, 851, 941, 1113
PflFI GACNNNGTC 1 cut(s) 1349
PfoI TCCNGGA 1 cut(s) 455
PkrI GCNGC 9 cut(s) 281, 292, 919, 937, 1060, 1063, 1096, 1099, 1255
PleI GAGTC 4 cut(s) 261, 751, 1280, 1358
PpsI GAGTC 4 cut(s) 261, 751, 1280, 1358
PscI ACATGT 1 cut(s) 1035
PshBI ATTAAT 1 cut(s) 972
Psp6I CCWGG 1 cut(s) 455
PspGI CCWGG 1 cut(s) 455
PspN4I GGNNCC 2 cut(s) 454, 1208
PspPI GGNCC 1 cut(s) 197
PstI CTGCAG 2 cut(s) 129, 1099
PstNI CAGNNNCTG 1 cut(s) 104
PsuI RGATCY 3 cut(s) 452, 499, 697
PsyI GACNNNGTC 1 cut(s) 1349
RsaI GTAC 3 cut(s) 311, 704, 1125
RsaNI GTAC 3 cut(s) 310, 703, 1124
RseI CAYNNNNRTG 1 cut(s) 1036
SaqAI TTAA 2 cut(s) 342, 972
SatI GCNGC 9 cut(s) 280, 291, 918, 936, 1059, 1062, 1095, 1098, 1254
Sau3AI GATC 5 cut(s) 191, 414, 452, 499, 697
Sau96I GGNCC 1 cut(s) 197
SchI GAGTC 4 cut(s) 262, 751, 1281, 1359
ScrFI CCNGG 1 cut(s) 457
SduI GDGCHC 3 cut(s) 319, 587, 725
SfaNI GCATC 3 cut(s) 904, 1196, 1251
SfcI CTRYAG 5 cut(s) 96, 105, 125, 1095, 1314
SmiMI CAYNNNNRTG 1 cut(s) 1036
SmlI CTYRAG 4 cut(s) 676, 828, 860, 1340
SmoI CTYRAG 4 cut(s) 676, 828, 860, 1340
Sse9I AATT 7 cut(s) 51, 229, 647, 793, 1065, 1299, 1320
SsiI CCGC 3 cut(s) 260, 277, 1058
SspI AATATT 1 cut(s) 746
SspMI CTAG 1 cut(s) 1142
StyD4I CCNGG 1 cut(s) 455
TaaI ACNGT 3 cut(s) 314, 540, 1197
TaiI ACGT 2 cut(s) 16, 414
TaqI TCGA 2 cut(s) 602, 1245
TasI AATT 7 cut(s) 51, 229, 647, 793, 1065, 1299, 1320
TatI WGTACW 1 cut(s) 702
TauI GCSGC 1 cut(s) 1061
TfiI GAWTC 5 cut(s) 475, 823, 851, 941, 1113
Tru1I TTAA 2 cut(s) 342, 972
Tru9I TTAA 2 cut(s) 342, 972
TscAI CASTG 7 cut(s) 129, 319, 425, 445, 452, 543, 673
TseFI GTSAC 2 cut(s) 17, 464
TseI GCWGC 8 cut(s) 279, 290, 917, 935, 1061, 1094, 1097, 1253
Tsp45I GTSAC 2 cut(s) 17, 464
TspDTI ATGAA 8 cut(s) 45, 208, 312, 393, 642, 654, 933, 1058
TspGWI ACGGA 3 cut(s) 260, 297, 1362
TspRI CASTG 7 cut(s) 129, 319, 425, 445, 452, 543, 673
Tth111I GACNNNGTC 1 cut(s) 1349
VneI GTGCAC 1 cut(s) 315
VspI ATTAAT 1 cut(s) 972
XagI CCTNNNNNAGG 1 cut(s) 392
XapI RAATTY 3 cut(s) 229, 793, 1320
XceI RCATGY 2 cut(s) 38, 1039
XspI CTAG 1 cut(s) 1142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.