Rorug07G0223400
BZIP Family

Sterol 3-beta-glucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0001805
Physical Location & Seq
Forward (+)
20328442 .. 20337813
9372 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0223400.1

Sequence Viewer

Length: 2664 bp
ATGGCGGAATCGGTCGAGCTTCCCAGTCGCATCGGAATCCTCCCCTTCAAGAACAAGGTTCTCTTGCCGGGAGCTATCATCCGAATTCGGTGCACTTCACCCAGCAGTGTGAAGCTGGTGGAGCAGGAGTTATGGCAGAGGGAAGAGAAGGGATTGATCGGCATTCTTCCCGTTCGAGACGCCGCTGAGGCAGCAACCGTGGGTCCTATGTTGTCTCAAGGCATCGGAAGCGATTCTGGTGAGCGGAGCTCAAAAGTTCAAGTTGGCACATCTGATTCTCAAAGAGTGGATGGCAAGAATCAGCAGGATATTCATTGGCATACCAGGGGAGTTGCTGCGCGTGCTCTGCATCTCTCAAGAGGAGTTGAGAAGCCAAGTGGGAGGGTTACATATGTAGTTGTCCTTGAAGGTTTATGTAGATTCAGCGTCCAGGAACTTAGCACAAGAGGAACATATTATACTGCACGGATATCTCCTCTTGAAATGACAAAGTCTGAAATGGAGCATGTGGAGCAAGACCCTGAATTCATCACATTGTCTCGACAGTTCAAAGCAACTGCTACTGAGCTTATTTCTGTTCTTGAGCAGAAGCAAAAAACTGGGGGAAGAACAAAAGTTCTTTTAGAGACTGTCCCGGTTCATAAGTTGGCAGATATATTTGTTGCTAGCTTTGAGATAAGTTTTGAGGAGCAACTGTCTATGTTAGATTCAGTTGATCTAAAAGTAAGACTTTCAAAAGCTACCGAGTTAGTCGACAGGCATTTGCAGTCTATACATGTAGCAGAGAAGATTACACAGAAAGTTGAGGGTCAATTATCAAAATCACAAAAAGAATTCCTTTTACGCCAGCAGATGAGAGCTATAAAGGAAGAGCTCGGTGACAATGATGATGATGAAGATGATGTAGCCGCCCTAGAAAGAAAGATGCAAAGTGCAGGAATGCCTTCAAATATCTGGAAGCATGCACAGAGGGAGTTAAGGAGGCTTAAAAAGATGCAACCCCAGCAACCTGGATATAATAGTTCACGTGTTTACCTGGAGCTACTTGCTGATCTTCCCTGGGAGAAGGCTACTGAAGAATTTGAGTTAGATTTAAGGGTTGCAAAAGAACGTCTTGACAGTGATCACTATGGTTTAGTCAAGGTCAAGCAGCGGATTATTGAATATTTGGCCGTTCGTAAGCTCAAACCAGATGCCAGAGGCCCTGTGTTGTGCTTCGTTGGCCCACCAGGTGTTGGGAAAACATCTTTGGCATCATCTATTGCTGCTGCCCTGGGCAGAAAGTTTATACGCATATCCCTTGGTGGTGTCAAGGATGAGGCTGATATTCGAGGGCATAGGAGGACATACATTGGAAGCATGCCAGGGAGACTCATTGATGGATTGAAGAGAGTATCTGTTTGCAACCCTGTCATGCTGCTAGATGAAATTGACAAGACCGGTTCTGATGTGCGTGGTGATCCGGCTTCGGCTCTATTGGAGGTTCTTGACCCTGAGCAAAACAAAACTTTCAATGATCACTATTTGAATGTTCCATTTGACCTTTCAAAGGTGATTTTTGTGGCAACTGCAAATAGGATGCAGCCTATTCCACCTCCACTCTTGGACAGGATGGAAGTTATTGAGTTGCCTGGGTATACACCTGAAGAAAAACTGAAGATAGCTATGCACCATTTAATTCCTCGAGTTCTTGATCAACACGGGTTAAGCACCGAGTTTCTTCAAATCCCAGAGGCGATGGTGAAACTTGTCATTCAGGGGTACACCAGAGAGGCTGGTGTTCGGAATCTGGAGAGGAATTTGGCTGCTTTGGCTCGTGCGGCAGCTGTGAGAGTTGCGGAGCATGAACAAGCTGTCTCAGTGAGCAAAGATGTGCACTCGCTTGCTTCACCGATAGTGGAAAGCAGACTAGCTGATGGAGGTGAAGTGGAAATGGAGGTTATTCCAATGGGTGCAACTAATCATGAGATATCAAGCACTTTCAAGATTTCTTCGCCACTGATTGTGGATGAGGATATGCTGGAAAAAGTATTAGGGCCTCCAAGGTTTGATGACAAGGAAGCTGCAGAACGGGTTGCAACACCTGGCGTATCTGTTGGGCTTGTTTGGACTTCTGTTGGTGGGGAGGTTCAGTTTGTGGAGGCTACAGCAATGGCGGGAAAGGGTGAATTACATCTCACTGGTCAGCTTGGAGATGTTATAAAAGAATCAGCACAGATAGCACTGACATGGGTAAGAGCTAGGGCAACAGATCTTATGTTGGCAGCATCGGATGAAACAAATCTTCTGGAGGGTCGGGATGTTCATATACATTTTCCCGCTGGCGCTGTACCTAAGGATGGGCCCTCCGCTGGTGTGACACTGGTAACTGCACTTGTTTCATTGTTCAGTCAGAAAAGAGTAAGGGCAGATACAGCAATGACTGGAGAAATGACATTGAGGGGTCTCGTACTACCGGTTGGTGGTATCAAGGATAAGGTGTTGGCAGCACACCGTTGTGGTATTAAAAGAGTCATTCTTCCAGAGAGAAATCTGAAGGACTTAACTGAGGTACCATCAGCAGTGCTTGCCGGTCTTGAGATTATAGTTGCAAAGCGAATGGAAGACGTATTAGAGCAGGCTTTTGAAGGTGGATGCCCTTGGAGACAGCATTCAAAGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

887

Amino Acids

97.72

Weight (kDa)

6.32

Isoelectric Point (pI)

37.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LON_substr_bdg PF02190 13 - 253 9.3e-29 ATP-dependent protease La (LON) substrate-binding domain
AAA PF00004 404 - 541 1.3e-25 ATPase family associated with various cellular activities (AAA)
AAA_5 PF07728 404 - 538 1.5e-07 AAA domain (dynein-related subfamily)
Lon_lid PF22667 566 - 617 2.7e-17 Lon protease AAA+ ATPase lid domain
Lon_C PF05362 669 - 875 7e-78 Lon protease (S16) C-terminal proteolytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2201
Acc65I GGTACC 1 cut(s) 2551
AccB1I GGYRCC 1 cut(s) 2551
AccB7I CCANNNNNTGG 1 cut(s) 1235
AccBSI CCGCTC 1 cut(s) 244
AccI GTMKAC 2 cut(s) 753, 1637
AccII CGCG 1 cut(s) 340
AclWI GGATC 1 cut(s) 1454
AcoI YGGCCR 1 cut(s) 1170
AcsI RAATTY 5 cut(s) 84, 524, 833, 1079, 1798
AcuI CTGAAG 4 cut(s) 1095, 1665, 1676, 2555
AcvI CACGTG 1 cut(s) 1028
AcyI GRCGYC 1 cut(s) 180
AdeI CACNNNGTG 1 cut(s) 1232
AfaI GTAC 4 cut(s) 1763, 2331, 2451, 2553
AfiI CCNNNNNNNGG 5 cut(s) 1235, 1549, 2339, 2351, 2462
AflIII ACRYGT 2 cut(s) 775, 1027
AgeI ACCGGT 2 cut(s) 1439, 2455
AjuI GAANNNNNNNTTGG 2 cut(s) 1232, 1264
AleI CACNNNNGTG 1 cut(s) 2496
Alw21I GWGCWC 5 cut(s) 95, 251, 346, 876, 1878
Alw26I GTCTC 8 cut(s) 171, 219, 543, 620, 1363, 1861, 2450, 2638
Alw44I GTGCAC 2 cut(s) 91, 1874
AlwI GGATC 1 cut(s) 1454
Ama87I CYCGRG 1 cut(s) 1683
AoxI GGCC 5 cut(s) 1170, 1201, 1222, 2036, 2342
ApaI GGGCCC 1 cut(s) 2346
ApaLI GTGCAC 2 cut(s) 91, 1874
ApoI RAATTY 5 cut(s) 84, 524, 833, 1079, 1798
AsiGI ACCGGT 2 cut(s) 1439, 2455
Asp700I GAANNNNTTC 2 cut(s) 232, 943
Asp718I GGTACC 1 cut(s) 2551
AspLEI GCGC 2 cut(s) 340, 2327
AspS9I GGNCC 6 cut(s) 203, 1202, 1223, 2036, 2342, 2343
AsuC2I CCSGG 2 cut(s) 69, 635
AsuHPI GGTGA 9 cut(s) 90, 251, 890, 1469, 1564, 1753, 1881, 1934, 2177
AsuNHI GCTAGC 1 cut(s) 665
AvaI CYCGRG 1 cut(s) 1683
AvaII GGWCC 1 cut(s) 203
AxyI CCTNAGG 1 cut(s) 2334
BaeGI GKGCMC 3 cut(s) 95, 1878, 2346
BaeI ACNNNNGTAYC 2 cut(s) 2535, 2568
BanI GGYRCC 1 cut(s) 2551
BanII GRGCYC 3 cut(s) 251, 876, 2346
BauI CACGAG 1 cut(s) 1815
BbrPI CACGTG 1 cut(s) 1028
BbsI GAAGAC 1 cut(s) 2610
Bbv12I GWGCWC 5 cut(s) 95, 251, 346, 876, 1878
BbvCI CCTCAGC 1 cut(s) 186
BccI CCATC 7 cut(s) 284, 1373, 1606, 1732, 1910, 2333, 2563
BceAI ACGGC 1 cut(s) 1157
BcgI CGANNNNNNTGC 2 cut(s) 72, 106
BclI TGATCA 3 cut(s) 1123, 1516, 1693
BcnI CCSGG 2 cut(s) 69, 635
BcoDI GTCTC 8 cut(s) 171, 219, 543, 620, 1363, 1861, 2450, 2638
BfaI CTAG 5 cut(s) 666, 914, 1421, 1910, 2241
BfmI CTRYAG 2 cut(s) 2064, 2145
BfoI RGCGCY 1 cut(s) 2328
BglI GCCNNNNNGGC 1 cut(s) 188
BglII AGATCT 1 cut(s) 2251
Bme18I GGWCC 1 cut(s) 203
BmeT110I CYCGRG 1 cut(s) 1683
BmgT120I GGNCC 6 cut(s) 203, 1202, 1223, 2036, 2342, 2343
BmiI GGNNCC 3 cut(s) 204, 2344, 2553
BmrI ACTGGG 2 cut(s) 18, 609
BmtI GCTAGC 1 cut(s) 669
BmuI ACTGGG 2 cut(s) 18, 609
BpiI GAAGAC 1 cut(s) 2610
BplI GAGNNNNNCTC 2 cut(s) 233, 265
BpmI CTGGAG 4 cut(s) 1058, 1811, 2309, 2445
Bpu10I CCTNAGC 2 cut(s) 186, 1494
BpuEI CTTGAG 4 cut(s) 201, 340, 602, 2597
BpuMI CCSGG 2 cut(s) 69, 635
BsaAI YACGTR 1 cut(s) 1028
BsaHI GRCGYC 1 cut(s) 180
BsaI GGTCTC 1 cut(s) 2450
BsaWI WCCGGW 2 cut(s) 1439, 2455
BsaXI ACNNNNNCTCC 8 cut(s) 113, 143, 680, 710, 1911, 1941, 2117, 2147
Bsc4I CCNNNNNNNGG 5 cut(s) 1235, 1549, 2339, 2351, 2462
Bse118I RCCGGY 3 cut(s) 1439, 2455, 2570
Bse1I ACTGG 5 cut(s) 24, 604, 2185, 2367, 2428
Bse21I CCTNAGG 1 cut(s) 2334
Bse3DI GCAATG 2 cut(s) 2157, 2424
BseLI CCNNNNNNNGG 5 cut(s) 1235, 1549, 2339, 2351, 2462
BseMI GCAATG 2 cut(s) 2157, 2424
BseMII CTCAG 5 cut(s) 177, 555, 1485, 1872, 2538
BseNI ACTGG 5 cut(s) 24, 604, 2185, 2367, 2428
BseRI GAGGAG 3 cut(s) 375, 465, 701
BseSI GKGCMC 3 cut(s) 95, 1878, 2346
BseYI CCCAGC 2 cut(s) 101, 1002
BsgI GTGCAG 3 cut(s) 447, 954, 2355
Bsh1236I CGCG 1 cut(s) 340
Bsh1285I CGRYCG 1 cut(s) 15
BshFI GGCC 5 cut(s) 1172, 1203, 1224, 2038, 2344
BshNI GGYRCC 1 cut(s) 2551
BshTI ACCGGT 2 cut(s) 1439, 2455
BsiEI CGRYCG 1 cut(s) 15
BsiHKAI GWGCWC 5 cut(s) 95, 251, 346, 876, 1878
BsiHKCI CYCGRG 1 cut(s) 1683
BsiSI CCGG 6 cut(s) 68, 635, 1440, 1463, 2456, 2571
BslFI GGGAC 1 cut(s) 617
BslI CCNNNNNNNGG 5 cut(s) 1235, 1549, 2339, 2351, 2462
BsmAI GTCTC 8 cut(s) 171, 219, 543, 620, 1363, 1861, 2450, 2638
BsmBI CGTCTC 1 cut(s) 171
BsmFI GGGAC 1 cut(s) 617
BsmI GAATGC 3 cut(s) 162, 945, 2650
BsnI GGCC 5 cut(s) 1172, 1203, 1224, 2038, 2344
Bso31I GGTCTC 1 cut(s) 2450
BsoBI CYCGRG 1 cut(s) 1683
Bsp120I GGGCCC 1 cut(s) 2342
Bsp1286I GDGCHC 6 cut(s) 95, 251, 346, 876, 1878, 2346
Bsp143I GATC 8 cut(s) 156, 715, 1051, 1123, 1459, 1516, 1693, 2251
BspANI GGCC 5 cut(s) 1172, 1203, 1224, 2038, 2344
BspCNI CTCAG 5 cut(s) 178, 556, 1486, 1871, 2539
BspFNI CGCG 1 cut(s) 340
BspHI TCATGA 1 cut(s) 1963
BspLI GGNNCC 3 cut(s) 204, 2344, 2553
BspMAI CTGCAG 1 cut(s) 2068
BspOI GCTAGC 1 cut(s) 669
BspPI GGATC 1 cut(s) 1454
BspQI GCTCTTC 1 cut(s) 864
BspT107I GGYRCC 1 cut(s) 2551
BspTNI GGTCTC 1 cut(s) 2450
BsrBI CCGCTC 1 cut(s) 244
BsrDI GCAATG 2 cut(s) 2157, 2424
BsrFI RCCGGY 3 cut(s) 1439, 2455, 2570
BsrI ACTGG 5 cut(s) 24, 604, 2185, 2367, 2428
BssAI RCCGGY 3 cut(s) 1439, 2455, 2570
BssMI GATC 8 cut(s) 156, 715, 1051, 1123, 1459, 1516, 1693, 2251
BssNAI GTATAC 1 cut(s) 1638
BssNI GRCGYC 1 cut(s) 180
BssSI CACGAG 1 cut(s) 1815
BssT1I CCWWGG 3 cut(s) 1300, 2042, 2639
Bst1107I GTATAC 1 cut(s) 1638
Bst2BI CACGAG 1 cut(s) 1815
Bst4CI ACNGT 6 cut(s) 199, 546, 631, 696, 1121, 2495
Bst6I CTCTTC 3 cut(s) 138, 864, 1382
BstACI GRCGYC 1 cut(s) 180
BstAPI GCANNNNNTGC 1 cut(s) 2567
BstBAI YACGTR 1 cut(s) 1028
BstC8I GCNNGC 9 cut(s) 342, 667, 848, 963, 1361, 1884, 2323, 2568, 2619
BstDEI CTNAG 7 cut(s) 186, 437, 564, 1494, 1858, 2334, 2547
BstDSI CCRYGG 1 cut(s) 198
BstENI CCTNNNNNAGG 1 cut(s) 1547
BstFNI CGCG 1 cut(s) 340
BstH2I RGCGCY 1 cut(s) 2328
BstHHI GCGC 2 cut(s) 340, 2327
BstKTI GATC 8 cut(s) 159, 718, 1054, 1126, 1462, 1519, 1696, 2254
BstMAI GTCTC 8 cut(s) 171, 219, 543, 620, 1363, 1861, 2450, 2638
BstMBI GATC 8 cut(s) 156, 715, 1051, 1123, 1459, 1516, 1693, 2251
BstMCI CGRYCG 1 cut(s) 15
BstNSI RCATGY 4 cut(s) 509, 779, 965, 1363
BstSFI CTRYAG 2 cut(s) 2064, 2145
BstSLI GKGCMC 3 cut(s) 95, 1878, 2346
BstUI CGCG 1 cut(s) 340
BstV2I GAAGAC 1 cut(s) 2610
BstX2I RGATCY 1 cut(s) 2251
BstXI CCANNNNNNTGG 1 cut(s) 1010
BstYI RGATCY 1 cut(s) 2251
BstZ17I GTATAC 1 cut(s) 1638
Bsu36I CCTNAGG 1 cut(s) 2334
BsuRI GGCC 5 cut(s) 1172, 1203, 1224, 2038, 2344
BtgI CCRYGG 1 cut(s) 198
BtgZI GCGATG 1 cut(s) 1751
BtsI GCAGTG 2 cut(s) 112, 2568
BtsIMutI CAGTG 8 cut(s) 112, 1126, 1866, 1997, 2178, 2222, 2360, 2568
Cac8I GCNNGC 9 cut(s) 342, 667, 848, 963, 1361, 1884, 2323, 2568, 2619
CciI TCATGA 1 cut(s) 1963
CfoI GCGC 2 cut(s) 340, 2327
Cfr10I RCCGGY 3 cut(s) 1439, 2455, 2570
Cfr13I GGNCC 6 cut(s) 203, 1202, 1223, 2036, 2342, 2343
CseI GACGC 2 cut(s) 188, 415
CsiI ACCWGGT 1 cut(s) 1228
Csp6I GTAC 4 cut(s) 1762, 2330, 2450, 2552
CspAI ACCGGT 2 cut(s) 1439, 2455
CviAII CATG 8 cut(s) 506, 776, 962, 1360, 1414, 1844, 1964, 2229
CviQI GTAC 4 cut(s) 1762, 2330, 2450, 2552
DdeI CTNAG 7 cut(s) 186, 437, 564, 1494, 1858, 2334, 2547
DpnI GATC 8 cut(s) 158, 717, 1053, 1125, 1461, 1518, 1695, 2253
DpnII GATC 8 cut(s) 156, 715, 1051, 1123, 1459, 1516, 1693, 2251
DraIII CACNNNGTG 1 cut(s) 1232
EaeI YGGCCR 1 cut(s) 1170
Eam1104I CTCTTC 3 cut(s) 138, 864, 1382
EarI CTCTTC 3 cut(s) 138, 864, 1382
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 2 cut(s) 249, 874
Eco130I CCWWGG 3 cut(s) 1300, 2042, 2639
Eco24I GRGCYC 3 cut(s) 251, 876, 2346
Eco31I GGTCTC 1 cut(s) 2450
Eco32I GATATC 2 cut(s) 471, 1971
Eco47I GGWCC 1 cut(s) 203
Eco53kI GAGCTC 2 cut(s) 249, 874
Eco57I CTGAAG 4 cut(s) 1095, 1665, 1676, 2555
Eco72I CACGTG 1 cut(s) 1028
Eco81I CCTNAGG 1 cut(s) 2334
Eco88I CYCGRG 1 cut(s) 1683
EcoICRI GAGCTC 2 cut(s) 249, 874
EcoNI CCTNNNNNAGG 1 cut(s) 1547
EcoO109I RGGNCCY 4 cut(s) 203, 1202, 2036, 2343
EcoRI GAATTC 3 cut(s) 84, 524, 833
EcoRV GATATC 2 cut(s) 471, 1971
EcoT14I CCWWGG 3 cut(s) 1300, 2042, 2639
EcoT38I GRGCYC 3 cut(s) 251, 876, 2346
ErhI CCWWGG 3 cut(s) 1300, 2042, 2639
Esp3I CGTCTC 1 cut(s) 171
FaeI CATG 8 cut(s) 509, 779, 965, 1363, 1417, 1847, 1967, 2232
FalI AAGNNNNNCTT 8 cut(s) 47, 79, 714, 746, 822, 854, 1098, 1130
FaqI GGGAC 1 cut(s) 617
FatI CATG 8 cut(s) 505, 775, 961, 1359, 1413, 1843, 1963, 2228
FauI CCCGC 2 cut(s) 2149, 2326
FauNDI CATATG 1 cut(s) 391
FbaI TGATCA 3 cut(s) 1123, 1516, 1693
FblI GTMKAC 2 cut(s) 753, 1637
FriOI GRGCYC 3 cut(s) 251, 876, 2346
FspBI CTAG 5 cut(s) 666, 914, 1421, 1910, 2241
GlaI GCGC 2 cut(s) 339, 2326
GsaI CCCAGC 2 cut(s) 105, 1006
GsuI CTGGAG 4 cut(s) 1058, 1811, 2309, 2445
HaeII RGCGCY 1 cut(s) 2328
HaeIII GGCC 5 cut(s) 1172, 1203, 1224, 2038, 2344
HapII CCGG 6 cut(s) 68, 635, 1440, 1463, 2456, 2571
HgaI GACGC 2 cut(s) 188, 415
HhaI GCGC 2 cut(s) 340, 2327
Hin1I GRCGYC 1 cut(s) 180
Hin1II CATG 8 cut(s) 509, 779, 965, 1363, 1417, 1847, 1967, 2232
Hin6I GCGC 2 cut(s) 338, 2325
HinP1I GCGC 2 cut(s) 338, 2325
HincII GTYRAC 1 cut(s) 754
HindII GTYRAC 1 cut(s) 754
HpaII CCGG 6 cut(s) 68, 635, 1440, 1463, 2456, 2571
HphI GGTGA 9 cut(s) 90, 251, 890, 1469, 1564, 1753, 1881, 1934, 2177
Hpy166II GTNNAC 7 cut(s) 93, 754, 1025, 1033, 1638, 1764, 1876
Hpy8I GTNNAC 7 cut(s) 93, 754, 1025, 1033, 1638, 1764, 1876
HpyAV CCTTC 7 cut(s) 55, 142, 401, 954, 1060, 2530, 2621
HpyCH4III ACNGT 6 cut(s) 199, 546, 631, 696, 1121, 2495
HpyCH4IV ACGT 3 cut(s) 1027, 1111, 2607
HpyF3I CTNAG 7 cut(s) 186, 437, 564, 1494, 1858, 2334, 2547
HpySE526I ACGT 3 cut(s) 1027, 1111, 2607
Hsp92I GRCGYC 1 cut(s) 180
Hsp92II CATG 8 cut(s) 509, 779, 965, 1363, 1417, 1847, 1967, 2232
HspAI GCGC 2 cut(s) 338, 2325
KpnI GGTACC 1 cut(s) 2555
Ksp22I TGATCA 3 cut(s) 1123, 1516, 1693
Kzo9I GATC 8 cut(s) 156, 715, 1051, 1123, 1459, 1516, 1693, 2251
LguI GCTCTTC 1 cut(s) 864
LmnI GCTCC 8 cut(s) 71, 121, 246, 502, 511, 688, 1039, 1840
MabI ACCWGGT 1 cut(s) 1228
MaeI CTAG 5 cut(s) 666, 914, 1421, 1910, 2241
MaeII ACGT 3 cut(s) 1027, 1111, 2607
MaeIII GTNAC 4 cut(s) 385, 878, 2356, 2365
MalI GATC 8 cut(s) 158, 717, 1053, 1125, 1461, 1518, 1695, 2253
MbiI CCGCTC 1 cut(s) 244
MboI GATC 8 cut(s) 156, 715, 1051, 1123, 1459, 1516, 1693, 2251
MflI RGATCY 1 cut(s) 2251
MhlI GDGCHC 6 cut(s) 95, 251, 346, 876, 1878, 2346
MluCI AATT 9 cut(s) 84, 524, 812, 833, 1079, 1428, 1677, 1798, 2168
MlyI GAGTC 2 cut(s) 1365, 2520
MroXI GAANNNNTTC 2 cut(s) 232, 943
MseI TTAA 7 cut(s) 977, 987, 1094, 1676, 1706, 2505, 2543
MslI CAYNNNNRTG 2 cut(s) 2227, 2496
MspA1I CMGCKG 5 cut(s) 185, 1153, 1826, 2321, 2351
MspI CCGG 6 cut(s) 68, 635, 1440, 1463, 2456, 2571
Mva1269I GAATGC 3 cut(s) 162, 945, 2650
MvnI CGCG 1 cut(s) 340
NciI CCSGG 2 cut(s) 69, 635
NdeI CATATG 1 cut(s) 391
NdeII GATC 8 cut(s) 156, 715, 1051, 1123, 1459, 1516, 1693, 2251
NheI GCTAGC 1 cut(s) 665
NlaIII CATG 8 cut(s) 509, 779, 965, 1363, 1417, 1847, 1967, 2232
NlaIV GGNNCC 3 cut(s) 204, 2344, 2553
NmuCI GTSAC 2 cut(s) 878, 2356
NspI RCATGY 4 cut(s) 509, 779, 965, 1363
OliI CACNNNNGTG 1 cut(s) 2496
PaeI GCATGC 2 cut(s) 965, 1363
PaeR7I CTCGAG 1 cut(s) 1683
PagI TCATGA 1 cut(s) 1963
PasI CCCWGGG 2 cut(s) 1059, 1273
PciI ACATGT 1 cut(s) 775
PciSI GCTCTTC 1 cut(s) 864
PctI GAATGC 3 cut(s) 162, 945, 2650
PdmI GAANNNNTTC 2 cut(s) 232, 943
PfeI GAWTC 9 cut(s) 8, 36, 233, 275, 298, 420, 707, 1786, 2207
PflFI GACNNNGTC 1 cut(s) 490
PflMI CCANNNNNTGG 1 cut(s) 1235
PfoI TCCNGGA 1 cut(s) 429
PinAI ACCGGT 2 cut(s) 1439, 2455
PleI GAGTC 2 cut(s) 1365, 2519
PmaCI CACGTG 1 cut(s) 1028
PmlI CACGTG 1 cut(s) 1028
PpsI GAGTC 2 cut(s) 1365, 2519
Ppu21I YACGTR 1 cut(s) 1028
PpuMI RGGWCCY 1 cut(s) 203
PscI ACATGT 1 cut(s) 775
PsiI TTATAA 1 cut(s) 2201
Psp124BI GAGCTC 2 cut(s) 251, 876
Psp5II RGGWCCY 1 cut(s) 203
PspCI CACGTG 1 cut(s) 1028
PspFI CCCAGC 2 cut(s) 101, 1002
PspN4I GGNNCC 3 cut(s) 204, 2344, 2553
PspOMI GGGCCC 1 cut(s) 2342
PspPI GGNCC 6 cut(s) 203, 1202, 1223, 2036, 2342, 2343
PspPPI RGGWCCY 1 cut(s) 203
PspXI VCTCGAGB 1 cut(s) 1683
PsrI GAACNNNNNNTAC 2 cut(s) 442, 474
PstI CTGCAG 1 cut(s) 2068
PsuI RGATCY 1 cut(s) 2251
PsyI GACNNNGTC 1 cut(s) 490
PvuII CAGCTG 1 cut(s) 1826
RsaI GTAC 4 cut(s) 1763, 2331, 2451, 2553
RsaNI GTAC 4 cut(s) 1762, 2330, 2450, 2552
RseI CAYNNNNRTG 2 cut(s) 2227, 2496
SacI GAGCTC 2 cut(s) 251, 876
SalI GTCGAC 1 cut(s) 752
SapI GCTCTTC 1 cut(s) 864
SaqAI TTAA 7 cut(s) 977, 987, 1094, 1676, 1706, 2505, 2543
Sau3AI GATC 8 cut(s) 156, 715, 1051, 1123, 1459, 1516, 1693, 2251
Sau96I GGNCC 6 cut(s) 203, 1202, 1223, 2036, 2342, 2343
SchI GAGTC 2 cut(s) 1365, 2520
SduI GDGCHC 6 cut(s) 95, 251, 346, 876, 1878, 2346
SexAI ACCWGGT 1 cut(s) 1228
SfcI CTRYAG 2 cut(s) 2064, 2145
Sfr274I CTCGAG 1 cut(s) 1683
SinI GGWCC 1 cut(s) 203
SlaI CTCGAG 1 cut(s) 1683
SmiMI CAYNNNNRTG 2 cut(s) 2227, 2496
SmlI CTYRAG 5 cut(s) 216, 355, 581, 1683, 2576
SmoI CTYRAG 5 cut(s) 216, 355, 581, 1683, 2576
SphI GCATGC 2 cut(s) 965, 1363
Sse9I AATT 9 cut(s) 84, 524, 812, 833, 1079, 1428, 1677, 1798, 2168
SspI AATATT 1 cut(s) 1166
SspMI CTAG 5 cut(s) 666, 914, 1421, 1910, 2241
SstI GAGCTC 2 cut(s) 251, 876
StyI CCWWGG 3 cut(s) 1300, 2042, 2639
TaaI ACNGT 6 cut(s) 199, 546, 631, 696, 1121, 2495
TaiI ACGT 3 cut(s) 1030, 1114, 2610
TaqI TCGA 6 cut(s) 15, 175, 541, 753, 1330, 1684
TasI AATT 9 cut(s) 84, 524, 812, 833, 1079, 1428, 1677, 1798, 2168
TauI GCSGC 3 cut(s) 185, 911, 1823
TfiI GAWTC 9 cut(s) 8, 36, 233, 275, 298, 420, 707, 1786, 2207
Tru1I TTAA 7 cut(s) 977, 987, 1094, 1676, 1706, 2505, 2543
Tru9I TTAA 7 cut(s) 977, 987, 1094, 1676, 1706, 2505, 2543
TscAI CASTG 8 cut(s) 112, 1126, 1866, 2004, 2185, 2229, 2367, 2568
TseFI GTSAC 2 cut(s) 878, 2356
Tsp45I GTSAC 2 cut(s) 878, 2356
TspDTI ATGAA 9 cut(s) 302, 517, 629, 909, 1440, 1860, 2289, 2294, 2370
TspGWI ACGGA 1 cut(s) 481
TspRI CASTG 8 cut(s) 112, 1126, 1866, 2004, 2185, 2229, 2367, 2568
Tth111I GACNNNGTC 1 cut(s) 490
Van91I CCANNNNNTGG 1 cut(s) 1235
VneI GTGCAC 2 cut(s) 91, 1874
VpaK11BI GGWCC 1 cut(s) 203
XagI CCTNNNNNAGG 1 cut(s) 1547
XapI RAATTY 5 cut(s) 84, 524, 833, 1079, 1798
XceI RCATGY 4 cut(s) 509, 779, 965, 1363
XhoI CTCGAG 1 cut(s) 1683
XmiI GTMKAC 2 cut(s) 753, 1637
XmnI GAANNNNTTC 2 cut(s) 232, 943
XspI CTAG 5 cut(s) 666, 914, 1421, 1910, 2241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.