Rorug07G0223600
BZIP Family

G-box-binding factor 4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0001805
Physical Location & Seq
Reverse (-)
20347768 .. 20350185
2418 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0223600.1

Sequence Viewer

Length: 603 bp
ATGTTCCGGCAAGCCTCACGTCTCTTGGGTCGAGCCTCTTGGCTGCGGCCACGACCCTTCTGCACAGAGGTCCCAGCAGCCACTCCATCAGCGGACCCAGCCTTTGTAGAGGCATGGAAGAAAGTGGCGCCCAACATCGACCCACCCAGGACTCCATCAACCTACATGGACCCTCGGCCTCCCATCCCTTCTTCCCTCCCTTCCAAAATGACAGTCAATTTCGTCCTCCCTTATTCTTCTGAGCTCTCTGGCAAAGAGGTCGACATGGTCATGATACCAGCAACAACCGGACAGATGGGTGTTTTGCCTGGCCATGTCGCAACCATTGCGGAGCTAAAGCCTGGAGTCTTATCAGTGCACGATGGGAGTGATGTGAAAAAGTATTTTATCAGCAGTGGATTTGCTTTTATTCATGCAAATTCTGTTGCTGATATAATTGCCGTGGAAGCTGTGCCAATTGACCAAATTGACGCAAGCTTGGTACAGAAGGGGCTTGCAGAGTTCACTCAGAAGCTCAGTTCAGCTACAACCGATTTGGAGAAAGCTGAAGCACAAATAGGCATCGATGTTCATAGTGCTCTTAACTCTGCTATCACTGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
bZIP_1

Protein Analysis

200

Amino Acids

21.33

Weight (kDa)

5.78

Isoelectric Point (pI)

37.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP-synt_DE_N PF02823 70 - 142 5.4e-17 ATP synthase, Delta/Epsilon chain, beta-sandwich domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 127
AccI GTMKAC 1 cut(s) 261
AciI CCGC 3 cut(s) 46, 92, 329
AcoI YGGCCR 2 cut(s) 47, 310
AcsI RAATTY 1 cut(s) 418
AcuI CTGAAG 1 cut(s) 567
AcyI GRCGYC 1 cut(s) 128
AfaI GTAC 1 cut(s) 483
AjiI CACGTC 1 cut(s) 20
AjnI CCWGG 3 cut(s) 146, 307, 340
AluBI AGCT 7 cut(s) 244, 334, 449, 477, 514, 524, 545
AluI AGCT 7 cut(s) 244, 334, 449, 477, 514, 524, 545
Alw21I GWGCWC 3 cut(s) 246, 360, 580
Alw26I GTCTC 1 cut(s) 26
Alw44I GTGCAC 1 cut(s) 356
AoxI GGCC 3 cut(s) 47, 176, 310
ApaLI GTGCAC 1 cut(s) 356
ApeKI GCWGC 2 cut(s) 43, 77
ApoI RAATTY 1 cut(s) 418
ArsI GACNNNNNNTTYG 4 cut(s) 86, 118, 198, 230
AspLEI GCGC 1 cut(s) 130
AspS9I GGNCC 3 cut(s) 70, 94, 169
AvaII GGWCC 3 cut(s) 70, 94, 169
BaeGI GKGCMC 1 cut(s) 360
BalI TGGCCA 1 cut(s) 312
BanI GGYRCC 1 cut(s) 127
BanII GRGCYC 1 cut(s) 246
Bbv12I GWGCWC 3 cut(s) 246, 360, 580
BbvI GCAGC 2 cut(s) 30, 89
BccI CCATC 5 cut(s) 94, 163, 191, 289, 356
BceAI ACGGC 1 cut(s) 425
BcgI CGANNNNNNTGC 4 cut(s) 42, 76, 241, 275
BciT130I CCWGG 3 cut(s) 148, 309, 342
BcoDI GTCTC 1 cut(s) 26
BfoI RGCGCY 1 cut(s) 131
BisI GCNGC 3 cut(s) 44, 47, 78
BlsI GCNGC 3 cut(s) 45, 48, 79
Bme1390I CCNGG 3 cut(s) 148, 309, 342
Bme18I GGWCC 3 cut(s) 70, 94, 169
BmgBI CACGTC 1 cut(s) 20
BmgT120I GGNCC 3 cut(s) 70, 94, 169
BmiI GGNNCC 4 cut(s) 72, 96, 129, 171
BmrFI CCNGG 3 cut(s) 148, 309, 342
BmsI GCATC 1 cut(s) 570
BpmI CTGGAG 1 cut(s) 363
Bsa29I ATCGAT 1 cut(s) 564
BsaHI GRCGYC 1 cut(s) 128
BsaJI CCNNGG 3 cut(s) 146, 173, 441
BsaWI WCCGGW 1 cut(s) 287
Bse1I ACTGG 1 cut(s) 601
Bse3DI GCAATG 1 cut(s) 324
BseBI CCWGG 3 cut(s) 148, 309, 342
BseCI ATCGAT 1 cut(s) 564
BseDI CCNNGG 3 cut(s) 146, 173, 441
BseGI GGATG 1 cut(s) 183
BseMI GCAATG 1 cut(s) 324
BseMII CTCAG 3 cut(s) 231, 521, 529
BseNI ACTGG 1 cut(s) 601
BseSI GKGCMC 1 cut(s) 360
BseXI GCAGC 2 cut(s) 30, 89
BseYI CCCAGC 2 cut(s) 73, 97
BsgI GTGCAG 1 cut(s) 46
BshFI GGCC 3 cut(s) 49, 178, 312
BshNI GGYRCC 1 cut(s) 127
BshVI ATCGAT 1 cut(s) 564
BsiHKAI GWGCWC 3 cut(s) 246, 360, 580
BsiSI CCGG 2 cut(s) 7, 288
BslFI GGGAC 1 cut(s) 56
BsmAI GTCTC 1 cut(s) 26
BsmBI CGTCTC 1 cut(s) 26
BsmFI GGGAC 1 cut(s) 56
BsnI GGCC 3 cut(s) 49, 178, 312
Bsp1286I GDGCHC 3 cut(s) 246, 360, 580
BspACI CCGC 3 cut(s) 46, 92, 329
BspANI GGCC 3 cut(s) 49, 178, 312
BspCNI CTCAG 3 cut(s) 232, 520, 528
BspDI ATCGAT 1 cut(s) 564
BspHI TCATGA 1 cut(s) 270
BspLI GGNNCC 4 cut(s) 72, 96, 129, 171
BspT107I GGYRCC 1 cut(s) 127
BsrDI GCAATG 1 cut(s) 324
BsrI ACTGG 1 cut(s) 601
BssECI CCNNGG 3 cut(s) 146, 173, 441
BssNI GRCGYC 1 cut(s) 128
Bst2UI CCWGG 3 cut(s) 148, 309, 342
Bst4CI ACNGT 1 cut(s) 214
BstACI GRCGYC 1 cut(s) 128
BstAPI GCANNNNNTGC 1 cut(s) 326
BstC8I GCNNGC 3 cut(s) 12, 475, 495
BstDEI CTNAG 3 cut(s) 240, 507, 515
BstDSI CCRYGG 1 cut(s) 441
BstF5I GGATG 1 cut(s) 183
BstH2I RGCGCY 1 cut(s) 131
BstHHI GCGC 1 cut(s) 130
BstMAI GTCTC 1 cut(s) 26
BstMWI GCNNNNNNNGC 3 cut(s) 98, 326, 446
BstNI CCWGG 3 cut(s) 148, 309, 342
BstSCI CCNGG 3 cut(s) 146, 307, 340
BstSLI GKGCMC 1 cut(s) 360
BstV1I GCAGC 2 cut(s) 30, 89
Bsu15I ATCGAT 1 cut(s) 564
BsuRI GGCC 3 cut(s) 49, 178, 312
BsuTUI ATCGAT 1 cut(s) 564
BtgI CCRYGG 1 cut(s) 441
BtrI CACGTC 1 cut(s) 20
BtsCI GGATG 1 cut(s) 183
BtsI GCAGTG 1 cut(s) 400
BtsIMutI CAGTG 3 cut(s) 360, 400, 594
Cac8I GCNNGC 3 cut(s) 12, 475, 495
CciI TCATGA 1 cut(s) 270
CfoI GCGC 1 cut(s) 130
Cfr13I GGNCC 3 cut(s) 70, 94, 169
ClaI ATCGAT 1 cut(s) 564
CseI GACGC 1 cut(s) 479
Csp6I GTAC 1 cut(s) 482
CviAII CATG 6 cut(s) 114, 166, 265, 271, 314, 413
CviQI GTAC 1 cut(s) 482
DdeI CTNAG 3 cut(s) 240, 507, 515
DinI GGCGCC 1 cut(s) 129
EaeI YGGCCR 2 cut(s) 47, 310
Ecl136II GAGCTC 1 cut(s) 244
Eco24I GRGCYC 1 cut(s) 246
Eco47I GGWCC 3 cut(s) 70, 94, 169
Eco53kI GAGCTC 1 cut(s) 244
Eco57I CTGAAG 1 cut(s) 567
EcoICRI GAGCTC 1 cut(s) 244
EcoO109I RGGNCCY 1 cut(s) 70
EcoRII CCWGG 3 cut(s) 146, 307, 340
EcoT38I GRGCYC 1 cut(s) 246
EgeI GGCGCC 1 cut(s) 129
EheI GGCGCC 1 cut(s) 129
Esp3I CGTCTC 1 cut(s) 26
FaeI CATG 6 cut(s) 117, 169, 268, 274, 317, 416
FaiI YATR 8 cut(s) 115, 167, 266, 272, 315, 414, 434, 573
FaqI GGGAC 1 cut(s) 56
FatI CATG 6 cut(s) 113, 165, 264, 270, 313, 412
FblI GTMKAC 1 cut(s) 261
Fnu4HI GCNGC 3 cut(s) 44, 47, 78
FokI GGATG 1 cut(s) 170
FriOI GRGCYC 1 cut(s) 246
Fsp4HI GCNGC 3 cut(s) 44, 47, 78
GlaI GCGC 1 cut(s) 129
GluI GCNGC 3 cut(s) 44, 47, 78
GsaI CCCAGC 2 cut(s) 77, 101
GsuI CTGGAG 1 cut(s) 363
HaeII RGCGCY 1 cut(s) 131
HaeIII GGCC 3 cut(s) 49, 178, 312
HapII CCGG 2 cut(s) 7, 288
HgaI GACGC 1 cut(s) 479
HhaI GCGC 1 cut(s) 130
Hin1I GRCGYC 1 cut(s) 128
Hin1II CATG 6 cut(s) 117, 169, 268, 274, 317, 416
Hin6I GCGC 1 cut(s) 128
HinP1I GCGC 1 cut(s) 128
HincII GTYRAC 1 cut(s) 262
HindII GTYRAC 1 cut(s) 262
HindIII AAGCTT 1 cut(s) 475
HinfI GANTC 2 cut(s) 151, 345
HpaII CCGG 2 cut(s) 7, 288
Hpy166II GTNNAC 3 cut(s) 262, 358, 504
Hpy188I TCNGA 2 cut(s) 241, 510
Hpy188III TCNNGA 1 cut(s) 271
Hpy8I GTNNAC 3 cut(s) 262, 358, 504
HpyAV CCTTC 4 cut(s) 67, 198, 210, 481
HpyCH4III ACNGT 1 cut(s) 214
HpyCH4IV ACGT 1 cut(s) 19
HpyCH4V TGCA 4 cut(s) 63, 358, 416, 497
HpyF10VI GCNNNNNNNGC 3 cut(s) 98, 326, 446
HpyF3I CTNAG 3 cut(s) 240, 507, 515
HpySE526I ACGT 1 cut(s) 19
Hsp92I GRCGYC 1 cut(s) 128
Hsp92II CATG 6 cut(s) 117, 169, 268, 274, 317, 416
HspAI GCGC 1 cut(s) 128
KasI GGCGCC 1 cut(s) 127
LmnI GCTCC 1 cut(s) 331
Lsp1109I GCAGC 2 cut(s) 30, 89
LweI GCATC 1 cut(s) 570
MaeII ACGT 1 cut(s) 19
MboII GAAGA 3 cut(s) 130, 183, 228
MfeI CAATTG 1 cut(s) 456
MhlI GDGCHC 3 cut(s) 246, 360, 580
MlsI TGGCCA 1 cut(s) 312
MluCI AATT 5 cut(s) 217, 418, 435, 456, 465
MluNI TGGCCA 1 cut(s) 312
Mly113I GGCGCC 1 cut(s) 128
MlyI GAGTC 2 cut(s) 145, 354
MnlI CCTC 9 cut(s) 25, 46, 61, 103, 183, 189, 206, 236, 250
Mox20I TGGCCA 1 cut(s) 312
MscI TGGCCA 1 cut(s) 312
MseI TTAA 1 cut(s) 582
MslI CAYNNNNRTG 1 cut(s) 269
Msp20I TGGCCA 1 cut(s) 312
MspA1I CMGCKG 1 cut(s) 92
MspI CCGG 2 cut(s) 7, 288
MspR9I CCNGG 3 cut(s) 148, 309, 342
MunI CAATTG 1 cut(s) 456
MvaI CCWGG 3 cut(s) 148, 309, 342
MwoI GCNNNNNNNGC 3 cut(s) 98, 326, 446
NarI GGCGCC 1 cut(s) 128
NlaIII CATG 6 cut(s) 117, 169, 268, 274, 317, 416
NlaIV GGNNCC 4 cut(s) 72, 96, 129, 171
NmeAIII GCCGAG 1 cut(s) 154
PagI TCATGA 1 cut(s) 270
PflFI GACNNNGTC 1 cut(s) 266
PkrI GCNGC 3 cut(s) 45, 48, 79
PleI GAGTC 2 cut(s) 145, 353
PluTI GGCGCC 1 cut(s) 131
PpsI GAGTC 2 cut(s) 145, 353
PpuMI RGGWCCY 1 cut(s) 70
Psp124BI GAGCTC 1 cut(s) 246
Psp5II RGGWCCY 1 cut(s) 70
Psp6I CCWGG 3 cut(s) 146, 307, 340
PspFI CCCAGC 2 cut(s) 73, 97
PspGI CCWGG 3 cut(s) 146, 307, 340
PspN4I GGNNCC 4 cut(s) 72, 96, 129, 171
PspPI GGNCC 3 cut(s) 70, 94, 169
PspPPI RGGWCCY 1 cut(s) 70
PsyI GACNNNGTC 1 cut(s) 266
RsaI GTAC 1 cut(s) 483
RsaNI GTAC 1 cut(s) 482
RseI CAYNNNNRTG 1 cut(s) 269
SacI GAGCTC 1 cut(s) 246
SalI GTCGAC 1 cut(s) 260
SaqAI TTAA 1 cut(s) 582
SatI GCNGC 3 cut(s) 44, 47, 78
Sau96I GGNCC 3 cut(s) 70, 94, 169
SchI GAGTC 2 cut(s) 145, 354
ScrFI CCNGG 3 cut(s) 148, 309, 342
SduI GDGCHC 3 cut(s) 246, 360, 580
SfaNI GCATC 1 cut(s) 570
SfoI GGCGCC 1 cut(s) 129
SinI GGWCC 3 cut(s) 70, 94, 169
SmiMI CAYNNNNRTG 1 cut(s) 269
Sse9I AATT 5 cut(s) 217, 418, 435, 456, 465
SsiI CCGC 3 cut(s) 46, 92, 329
SspDI GGCGCC 1 cut(s) 127
SstI GAGCTC 1 cut(s) 246
StyD4I CCNGG 3 cut(s) 146, 307, 340
TaaI ACNGT 1 cut(s) 214
TaiI ACGT 1 cut(s) 22
TaqI TCGA 4 cut(s) 31, 138, 261, 564
TasI AATT 5 cut(s) 217, 418, 435, 456, 465
TauI GCSGC 1 cut(s) 49
Tru1I TTAA 1 cut(s) 582
Tru9I TTAA 1 cut(s) 582
TscAI CASTG 3 cut(s) 360, 400, 601
TseI GCWGC 2 cut(s) 43, 77
TspDTI ATGAA 2 cut(s) 401, 560
TspRI CASTG 3 cut(s) 360, 400, 601
Tth111I GACNNNGTC 1 cut(s) 266
VneI GTGCAC 1 cut(s) 356
VpaK11BI GGWCC 3 cut(s) 70, 94, 169
XapI RAATTY 1 cut(s) 418
XmiI GTMKAC 1 cut(s) 261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.