MD08G1224800.v1.1

Sterol 3-beta-glucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0014580
Physical Location & Seq
Forward (+)
28989732 .. 29006868
17137 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1224800.v1.1.491

Sequence Viewer

Length: 1533 bp
ATGGAGGTCGAGACGGAGAGGACGAAACCTGCTGCTGCGTTCATGGCCTTTGGTACCAAAGGCGATGTCTATCCAATTGCTGCCATTGCGGCGGCTTTTGCTTGTGACCAGATACTGTACGACGTCGTCCTTATCACGCATTCAGCACATGAGAACTTACGTTGTCATTTAGCCGAAAAACATGTTTCGTATCTTCCAATTTCATCACCACCTGTTGTCCCAATCCATCGGGAACATGATAGTACAGAAGAACCAGGTTCTTTGGAGAAAATCATGTTTTCTGCTGAACACAGACAAGAATGTTGTTTGGCAGTGGAGAAGATATTTGGATCAGGCCCGAGCATGGAGGGTGATTTTATACTGATCAATTTCTTTGCTTTGGAAGGATGGAGTATTGCAGAACTTTTTCATGTCCGTTGCATAGTTGCTGCTCCTTATGTTGTTCCATATAGTGCACCTTCATCGTTTGAGCGCCAATTTGAAAAAGAACTTCCACTTTTATATAAATACCTACAGGAAGCTCACAACACTAAGGTTTGTTGGAAAGATGTGATCCACTGGATGTGGCCGCTTTTCAGCGAGTATTGGGGATCCTGGAGATGTGACGATTTGAATTTGAGTCCTTGCCCTTTTACGGATCCAGTAACAGGTTTACCCACTTGGCATGATAGGCCTCCATCTCCGCTTCTTCTGTATGGATTTAGCAAAGAAATAGTCGAGTGCCCTGGTTATTGGCCATCAAATGTTCGGGTTTGTGGCTTTTGGTTTCTCCCAAATGAATGGCAGTTTTCGTGTAAGCAGTGCAGTGAACTTTCCAGAATTGTTTCTTCAGAGCATCTTTACACAAAAGATGAAATGTGTTCAGCTCATGTTCAGTTAGAATATTTTTTGAAGACCCCAGTGTCTATGCCACCTATTTTCATAGGCCTGAGCTCAATTGGAAGCATGGGATTTTTAAAAAACACTCAAGCGTTTCTTCGTCTTCTCCAAACTGTCTTAGAGATAACAAATTACCGCTTTATCCTCTTTACAGCCGGCTACATACCTTTAGATGCAGCAATCCGAGCAGTTGCTGCTGAGTCACCACCATATGTAACCCACAAGCAGTTTAATGACCAGTGCATTTCTCTCTTCAATGACCGACTCTTTTGCTTTTCTGGAACTGTACCGTACAAGTGGCTGTTTACTCGATGTGCTGCTGCAATTCATCATGGGGGAAGTGGCACTACTGCTGCAGCACTATTGGCAGGAATCCCACAGGTAGTCTGTCCATTTGTGCTAGATCAGTTTTACTGGGCTGAAAGAATGTTTTGGCTTGGAGTAGCACCTGAGCCATTGAAAAGAACCCATTTACTTCCAGAAGAAAGTGATGAGAAAATAGCGGCAAATTTTCTATCGAGGGCCATACATGATGCATTATCCCCTGAAATTAAAGAACGTGCTGTGGAAGTTTCTAAGAGAATATCTCTTGAGGATGGTGTCTCAAACGCGGTCAAATATATCAAGGAAGAGATCATTGGTGGTTCAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

511

Amino Acids

57.59

Weight (kDa)

5.59

Isoelectric Point (pI)

45.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EryCIII-like_C PF06722 387 - 441 3.1e-12 Erythromycin biosynthesis protein CIII-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014580)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G24750
fragaria_vesca FvH4_5g30191 FvH4_5g30191
malus_domestica MD08G1224800.v1.1
prunus_persica Prupe.1G562100_v2.0.a1
pyrus_communis pycom08g19590
rosa_chinensis RchiOBHm_Chr7g0224451
rosa_laevigata RLG00000001894
rosa_multiflora Rmu_sc0003894.1_g000001
rosa_roxburghii Rroxscaffold_3G00234750
rosa_rugosa Rorug07G0223300 Rorug07G0223400
rosa_samantha Rh7AG379900 Rh7BG359500 Rh7CG399400 Rh7DG369100
rosa_wichuraiana Rw7G031180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 901
AatII GACGTC 1 cut(s) 126
Acc36I ACCTGC 1 cut(s) 37
Acc65I GGTACC 1 cut(s) 53
AccB1I GGYRCC 1 cut(s) 53
AccII CGCG 1 cut(s) 1490
AciI CCGC 7 cut(s) 89, 92, 569, 683, 1015, 1382, 1490
AclWI GGATC 6 cut(s) 337, 547, 585, 598, 632, 645
AcoI YGGCCR 2 cut(s) 566, 734
AcsI RAATTY 2 cut(s) 613, 1387
AcuI CTGAAG 1 cut(s) 813
AcyI GRCGYC 1 cut(s) 123
AfaI GTAC 5 cut(s) 55, 119, 244, 1167, 1172
AfiI CCNNNNNNNGG 3 cut(s) 343, 634, 647
AflIII ACRYGT 1 cut(s) 181
AgsI TTSAA 6 cut(s) 482, 613, 892, 1135, 1339, 1527
AjnI CCWGG 3 cut(s) 253, 593, 724
AjuI GAANNNNNNNTTGG 2 cut(s) 1500, 1532
AluBI AGCT 4 cut(s) 521, 866, 933, 1530
AluI AGCT 4 cut(s) 521, 866, 933, 1530
Alw21I GWGCWC 2 cut(s) 457, 935
Alw26I GTCTC 2 cut(s) 5, 1486
Alw44I GTGCAC 1 cut(s) 453
AlwI GGATC 6 cut(s) 337, 547, 585, 598, 632, 645
AlwNI CAGNNNCTG 2 cut(s) 115, 1073
Ama87I CYCGRG 1 cut(s) 337
AoxI GGCC 7 cut(s) 45, 334, 566, 671, 734, 925, 1401
ApaLI GTGCAC 1 cut(s) 453
ApoI RAATTY 2 cut(s) 613, 1387
ArsI GACNNNNNNTTYG 4 cut(s) 51, 83, 699, 731
Asp700I GAANNNNTTC 2 cut(s) 405, 823
Asp718I GGTACC 1 cut(s) 53
AspLEI GCGC 1 cut(s) 474
AspS9I GGNCC 2 cut(s) 335, 1401
AsuHPI GGTGA 3 cut(s) 198, 362, 1074
AvaI CYCGRG 1 cut(s) 337
BaeGI GKGCMC 2 cut(s) 457, 725
BalI TGGCCA 1 cut(s) 736
BamHI GGATCC 2 cut(s) 590, 637
BanI GGYRCC 1 cut(s) 53
BanII GRGCYC 1 cut(s) 935
BarI GAAGNNNNNNTAC 2 cut(s) 1210, 1242
BbsI GAAGAC 2 cut(s) 899, 974
Bbv12I GWGCWC 2 cut(s) 457, 935
BccI CCATC 5 cut(s) 234, 381, 685, 745, 1469
BcgI CGANNNNNNTGC 4 cut(s) 444, 478, 1131, 1165
BciT130I CCWGG 3 cut(s) 255, 595, 726
BclI TGATCA 1 cut(s) 363
BcoDI GTCTC 2 cut(s) 5, 1486
BfaI CTAG 1 cut(s) 1280
BfmI CTRYAG 2 cut(s) 512, 1233
BfoI RGCGCY 1 cut(s) 475
BfuAI ACCTGC 1 cut(s) 37
BglI GCCNNNNNGGC 1 cut(s) 89
Bme1390I CCNGG 3 cut(s) 255, 595, 726
BmeT110I CYCGRG 1 cut(s) 337
BmgT120I GGNCC 2 cut(s) 335, 1401
BmiI GGNNCC 3 cut(s) 55, 592, 639
BmrFI CCNGG 3 cut(s) 255, 595, 726
BmrI ACTGGG 2 cut(s) 893, 1303
BmsI GCATC 3 cut(s) 844, 1042, 1402
BmuI ACTGGG 2 cut(s) 893, 1303
BpiI GAAGAC 2 cut(s) 899, 974
BplI GAGNNNNNCTC 2 cut(s) 1450, 1482
BpmI CTGGAG 1 cut(s) 616
Bpu10I CCTNAGC 2 cut(s) 929, 1329
BpuEI CTTGAG 2 cut(s) 951, 1490
BsaBI GATNNNNATC 1 cut(s) 69
BsaHI GRCGYC 1 cut(s) 123
BsaJI CCNNGG 1 cut(s) 724
Bsc4I CCNNNNNNNGG 3 cut(s) 343, 634, 647
Bse118I RCCGGY 1 cut(s) 1034
Bse1I ACTGG 5 cut(s) 563, 641, 899, 1117, 1298
Bse3DI GCAATG 1 cut(s) 84
Bse8I GATNNNNATC 1 cut(s) 69
BseBI CCWGG 3 cut(s) 255, 595, 726
BseDI CCNNGG 1 cut(s) 724
BseGI GGATG 3 cut(s) 392, 567, 1480
BseJI GATNNNNATC 1 cut(s) 69
BseLI CCNNNNNNNGG 3 cut(s) 343, 634, 647
BseMI GCAATG 1 cut(s) 84
BseMII CTCAG 3 cut(s) 920, 1068, 1320
BseNI ACTGG 5 cut(s) 563, 641, 899, 1117, 1298
BseSI GKGCMC 2 cut(s) 457, 725
BsgI GTGCAG 1 cut(s) 823
Bsh1236I CGCG 1 cut(s) 1490
BshFI GGCC 7 cut(s) 47, 336, 568, 673, 736, 927, 1403
BshNI GGYRCC 1 cut(s) 53
BsiHKAI GWGCWC 2 cut(s) 457, 935
BsiHKCI CYCGRG 1 cut(s) 337
BsiSI CCGG 1 cut(s) 1035
BslFI GGGAC 1 cut(s) 203
BslI CCNNNNNNNGG 3 cut(s) 343, 634, 647
BsmAI GTCTC 2 cut(s) 5, 1486
BsmBI CGTCTC 1 cut(s) 5
BsmFI GGGAC 1 cut(s) 203
BsmI GAATGC 1 cut(s) 139
BsnI GGCC 7 cut(s) 47, 336, 568, 673, 736, 927, 1403
BsoBI CYCGRG 1 cut(s) 337
Bsp1286I GDGCHC 3 cut(s) 457, 725, 935
Bsp143I GATC 7 cut(s) 329, 363, 552, 590, 637, 1282, 1512
BspACI CCGC 7 cut(s) 89, 92, 569, 683, 1015, 1382, 1490
BspANI GGCC 7 cut(s) 47, 336, 568, 673, 736, 927, 1403
BspCNI CTCAG 3 cut(s) 921, 1069, 1321
BspFNI CGCG 1 cut(s) 1490
BspLI GGNNCC 3 cut(s) 55, 592, 639
BspMAI CTGCAG 1 cut(s) 1237
BspMI ACCTGC 1 cut(s) 37
BspPI GGATC 6 cut(s) 337, 547, 585, 598, 632, 645
BspT107I GGYRCC 1 cut(s) 53
BsrDI GCAATG 1 cut(s) 84
BsrFI RCCGGY 1 cut(s) 1034
BsrI ACTGG 5 cut(s) 563, 641, 899, 1117, 1298
BssAI RCCGGY 1 cut(s) 1034
BssECI CCNNGG 1 cut(s) 724
BssMI GATC 7 cut(s) 329, 363, 552, 590, 637, 1282, 1512
BssNI GRCGYC 1 cut(s) 123
Bst2UI CCWGG 3 cut(s) 255, 595, 726
Bst4CI ACNGT 4 cut(s) 117, 994, 1165, 1170
Bst6I CTCTTC 2 cut(s) 1136, 1503
BstACI GRCGYC 1 cut(s) 123
BstAPI GCANNNNNTGC 1 cut(s) 1073
BstC8I GCNNGC 1 cut(s) 1036
BstDEI CTNAG 6 cut(s) 531, 929, 997, 1077, 1329, 1455
BstF5I GGATG 3 cut(s) 392, 567, 1480
BstFNI CGCG 1 cut(s) 1490
BstH2I RGCGCY 1 cut(s) 475
BstHHI GCGC 1 cut(s) 474
BstKTI GATC 7 cut(s) 332, 366, 555, 593, 640, 1285, 1515
BstMAI GTCTC 2 cut(s) 5, 1486
BstMBI GATC 7 cut(s) 329, 363, 552, 590, 637, 1282, 1512
BstMWI GCNNNNNNNGC 8 cut(s) 44, 86, 89, 98, 670, 1064, 1073, 1244
BstNI CCWGG 3 cut(s) 255, 595, 726
BstNSI RCATGY 1 cut(s) 185
BstSCI CCNGG 3 cut(s) 253, 593, 724
BstSFI CTRYAG 2 cut(s) 512, 1233
BstSLI GKGCMC 2 cut(s) 457, 725
BstUI CGCG 1 cut(s) 1490
BstV2I GAAGAC 2 cut(s) 899, 974
BstX2I RGATCY 2 cut(s) 590, 637
BstXI CCANNNNNNTGG 1 cut(s) 780
BstYI RGATCY 2 cut(s) 590, 637
BsuRI GGCC 7 cut(s) 47, 336, 568, 673, 736, 927, 1403
BtgZI GCGATG 1 cut(s) 78
BtsCI GGATG 3 cut(s) 392, 567, 1480
BtsI GCAGTG 3 cut(s) 318, 806, 811
BtsIMutI CAGTG 6 cut(s) 318, 556, 806, 811, 906, 1124
BveI ACCTGC 1 cut(s) 37
Cac8I GCNNGC 1 cut(s) 1036
CaiI CAGNNNCTG 2 cut(s) 115, 1073
CfoI GCGC 1 cut(s) 474
Cfr10I RCCGGY 1 cut(s) 1034
Cfr13I GGNCC 2 cut(s) 335, 1401
CsiI ACCWGGT 1 cut(s) 253
Csp6I GTAC 5 cut(s) 54, 118, 243, 1166, 1171
CviQI GTAC 5 cut(s) 54, 118, 243, 1166, 1171
DdeI CTNAG 6 cut(s) 531, 929, 997, 1077, 1329, 1455
DpnI GATC 7 cut(s) 331, 365, 554, 592, 639, 1284, 1514
DpnII GATC 7 cut(s) 329, 363, 552, 590, 637, 1282, 1512
DraI TTTAAA 1 cut(s) 957
DrdI GACNNNNNNGTC 1 cut(s) 901
DseDI GACNNNNNNGTC 1 cut(s) 901
EaeI YGGCCR 2 cut(s) 566, 734
Eam1104I CTCTTC 2 cut(s) 1136, 1503
EarI CTCTTC 2 cut(s) 1136, 1503
Ecl136II GAGCTC 1 cut(s) 933
Eco147I AGGCCT 2 cut(s) 673, 927
Eco24I GRGCYC 1 cut(s) 935
Eco53kI GAGCTC 1 cut(s) 933
Eco57I CTGAAG 1 cut(s) 813
Eco88I CYCGRG 1 cut(s) 337
EcoICRI GAGCTC 1 cut(s) 933
EcoRII CCWGG 3 cut(s) 253, 593, 724
EcoT22I ATGCAT 1 cut(s) 1417
EcoT38I GRGCYC 1 cut(s) 935
Esp3I CGTCTC 1 cut(s) 5
FalI AAGNNNNNCTT 2 cut(s) 960, 992
FaqI GGGAC 1 cut(s) 203
FauNDI CATATG 1 cut(s) 1090
FbaI TGATCA 1 cut(s) 363
FokI GGATG 3 cut(s) 399, 574, 1487
FriOI GRGCYC 1 cut(s) 935
FspBI CTAG 1 cut(s) 1280
GlaI GCGC 1 cut(s) 473
GsuI CTGGAG 1 cut(s) 616
HaeII RGCGCY 1 cut(s) 475
HaeIII GGCC 7 cut(s) 47, 336, 568, 673, 736, 927, 1403
HapII CCGG 1 cut(s) 1035
HhaI GCGC 1 cut(s) 474
Hin1I GRCGYC 1 cut(s) 123
Hin6I GCGC 1 cut(s) 472
HinP1I GCGC 1 cut(s) 472
HinfI GANTC 4 cut(s) 619, 1079, 1143, 1251
HpaII CCGG 1 cut(s) 1035
HphI GGTGA 3 cut(s) 198, 362, 1074
Hpy166II GTNNAC 4 cut(s) 455, 653, 809, 1185
Hpy188I TCNGA 2 cut(s) 832, 1064
Hpy188III TCNNGA 6 cut(s) 10, 230, 816, 1158, 1358, 1469
Hpy8I GTNNAC 4 cut(s) 455, 653, 809, 1185
Hpy99I CGWCG 2 cut(s) 125, 128
HpyAV CCTTC 2 cut(s) 377, 468
HpyCH4III ACNGT 4 cut(s) 117, 994, 1165, 1170
HpyCH4IV ACGT 3 cut(s) 123, 160, 1438
HpyCH4V TGCA 9 cut(s) 398, 420, 455, 804, 1055, 1122, 1202, 1235, 1415
HpyF10VI GCNNNNNNNGC 8 cut(s) 44, 86, 89, 98, 670, 1064, 1073, 1244
HpyF3I CTNAG 6 cut(s) 531, 929, 997, 1077, 1329, 1455
HpySE526I ACGT 3 cut(s) 123, 160, 1438
Hsp92I GRCGYC 1 cut(s) 123
HspAI GCGC 1 cut(s) 472
KpnI GGTACC 1 cut(s) 57
KroI GCCGGC 1 cut(s) 1034
KroNI GCCGGC 1 cut(s) 1036
Ksp22I TGATCA 1 cut(s) 363
Kzo9I GATC 7 cut(s) 329, 363, 552, 590, 637, 1282, 1512
LmnI GCTCC 1 cut(s) 436
LweI GCATC 3 cut(s) 844, 1042, 1402
MabI ACCWGGT 1 cut(s) 253
MaeI CTAG 1 cut(s) 1280
MaeII ACGT 3 cut(s) 123, 160, 1438
MaeIII GTNAC 5 cut(s) 104, 602, 643, 1080, 1093
MalI GATC 7 cut(s) 331, 365, 554, 592, 639, 1284, 1514
MboI GATC 7 cut(s) 329, 363, 552, 590, 637, 1282, 1512
MfeI CAATTG 2 cut(s) 75, 936
MflI RGATCY 2 cut(s) 590, 637
MhlI GDGCHC 3 cut(s) 457, 725, 935
MlsI TGGCCA 1 cut(s) 736
MluNI TGGCCA 1 cut(s) 736
MlyI GAGTC 3 cut(s) 628, 1088, 1137
MmeI TCCRAC 1 cut(s) 521
MnlI CCTC 6 cut(s) 12, 340, 684, 1034, 1392, 1465
Mox20I TGGCCA 1 cut(s) 736
Mph1103I ATGCAT 1 cut(s) 1417
MroNI GCCGGC 1 cut(s) 1034
MroXI GAANNNNTTC 2 cut(s) 405, 823
MscI TGGCCA 1 cut(s) 736
MseI TTAA 3 cut(s) 956, 1110, 1431
Msp20I TGGCCA 1 cut(s) 736
MspI CCGG 1 cut(s) 1035
MspR9I CCNGG 3 cut(s) 255, 595, 726
MunI CAATTG 2 cut(s) 75, 936
Mva1269I GAATGC 1 cut(s) 139
MvaI CCWGG 3 cut(s) 255, 595, 726
MvnI CGCG 1 cut(s) 1490
MwoI GCNNNNNNNGC 8 cut(s) 44, 86, 89, 98, 670, 1064, 1073, 1244
NaeI GCCGGC 1 cut(s) 1036
NdeI CATATG 1 cut(s) 1090
NdeII GATC 7 cut(s) 329, 363, 552, 590, 637, 1282, 1512
NgoMIV GCCGGC 1 cut(s) 1034
NlaIV GGNNCC 3 cut(s) 55, 592, 639
NmuCI GTSAC 3 cut(s) 104, 602, 1080
NsiI ATGCAT 1 cut(s) 1417
NspI RCATGY 1 cut(s) 185
PceI AGGCCT 2 cut(s) 673, 927
PciI ACATGT 1 cut(s) 181
PcsI WCGNNNNNNNCGW 1 cut(s) 20
PctI GAATGC 1 cut(s) 139
PdiI GCCGGC 1 cut(s) 1036
PdmI GAANNNNTTC 2 cut(s) 405, 823
PfeI GAWTC 1 cut(s) 1251
PflFI GACNNNGTC 1 cut(s) 125
PfoI TCCNGGA 1 cut(s) 593
PleI GAGTC 3 cut(s) 627, 1087, 1137
PpsI GAGTC 3 cut(s) 627, 1087, 1137
PscI ACATGT 1 cut(s) 181
Psp124BI GAGCTC 1 cut(s) 935
Psp6I CCWGG 3 cut(s) 253, 593, 724
PspGI CCWGG 3 cut(s) 253, 593, 724
PspN4I GGNNCC 3 cut(s) 55, 592, 639
PspPI GGNCC 2 cut(s) 335, 1401
PstI CTGCAG 1 cut(s) 1237
PstNI CAGNNNCTG 2 cut(s) 115, 1073
PsuI RGATCY 2 cut(s) 590, 637
PsyI GACNNNGTC 1 cut(s) 125
RsaI GTAC 5 cut(s) 55, 119, 244, 1167, 1172
RsaNI GTAC 5 cut(s) 54, 118, 243, 1166, 1171
SacI GAGCTC 1 cut(s) 935
SaqAI TTAA 3 cut(s) 956, 1110, 1431
Sau3AI GATC 7 cut(s) 329, 363, 552, 590, 637, 1282, 1512
Sau96I GGNCC 2 cut(s) 335, 1401
SchI GAGTC 3 cut(s) 628, 1088, 1137
ScrFI CCNGG 3 cut(s) 255, 595, 726
SduI GDGCHC 3 cut(s) 457, 725, 935
SexAI ACCWGGT 1 cut(s) 253
SfaNI GCATC 3 cut(s) 844, 1042, 1402
SfcI CTRYAG 2 cut(s) 512, 1233
SmlI CTYRAG 2 cut(s) 966, 1469
SmoI CTYRAG 2 cut(s) 966, 1469
SseBI AGGCCT 2 cut(s) 673, 927
SsiI CCGC 7 cut(s) 89, 92, 569, 683, 1015, 1382, 1490
SspI AATATT 1 cut(s) 884
SspMI CTAG 1 cut(s) 1280
SstI GAGCTC 1 cut(s) 935
StuI AGGCCT 2 cut(s) 673, 927
StyD4I CCNGG 3 cut(s) 253, 593, 724
TaaI ACNGT 4 cut(s) 117, 994, 1165, 1170
TaiI ACGT 3 cut(s) 126, 163, 1441
TaqI TCGA 4 cut(s) 9, 717, 1189, 1397
TaqII GACCGA 1 cut(s) 1155
TatI WGTACW 1 cut(s) 242
TauI GCSGC 4 cut(s) 92, 95, 571, 1385
TfiI GAWTC 1 cut(s) 1251
Tru1I TTAA 3 cut(s) 956, 1110, 1431
Tru9I TTAA 3 cut(s) 956, 1110, 1431
TscAI CASTG 6 cut(s) 318, 563, 806, 811, 906, 1124
TseFI GTSAC 3 cut(s) 104, 602, 1080
Tsp45I GTSAC 3 cut(s) 104, 602, 1080
TspDTI ATGAA 8 cut(s) 31, 192, 398, 450, 792, 867, 910, 1196
TspGWI ACGGA 3 cut(s) 29, 404, 650
TspRI CASTG 6 cut(s) 318, 563, 806, 811, 906, 1124
Tth111I GACNNNGTC 1 cut(s) 125
VneI GTGCAC 1 cut(s) 453
XapI RAATTY 2 cut(s) 613, 1387
XceI RCATGY 1 cut(s) 185
XmnI GAANNNNTTC 2 cut(s) 405, 823
XspI CTAG 1 cut(s) 1280
ZraI GACGTC 1 cut(s) 124
Zsp2I ATGCAT 1 cut(s) 1417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.