pycom08g19590

Sterol 3-beta-glucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0014580
Physical Location & Seq
Forward (+)
19609669 .. 19618198
8530 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g19590.15

Sequence Viewer

Length: 1662 bp
ATGGTCTGCATTTGCACTAGCCAGCAGCGAATAGTCATGGAGGTTGAAACGGAGAGGACGAAACCTGCTGCTGCGTTCATGGCCTTCGGTACCAAGGGCGATGTCTATCCAATTGCTGCCATTGCGGCGGCTTTTGCTTGTGACCAGAGCCAGTACGACGTCGTCCTCATCACGCATTCAGCGCATGAGAACTTACGTTGTCATTTGGCCGAAAAGCATGTTTCTTATCTTCCAATTTCATCACCACCTGTTGTCCCAATCCATCAGGAACACGATAGTACAGAAGAACCAGGTTCTTTGGAGAAAATCATGTTTTCTGCTGAACACAGACAAGAATGTTGTTTGGCAGTGGAGACAATATTTGGATCAGGCCCGAGCATGGAGGGTGATTTTATACTGATCAATTTCTTTGCTTTGGAAGGATGGAGTATTGCAGAACTTTTTCATGTCCGTTGCATAGTTGCTGCTCCTTATGTTGTTCCATATAGTGCACCTTCGTCGTTTGAGCGCCAATTTGAAAAAGAACTTCCACTTTTATATAAATACCTACAGGAAGCTCACAACACTAAGGTTTGTTGGAAAGATGTGATCCACTGGATGTGGCCACTTTTCAGCGAGTATTGGGGATCCTGGCGATGTGACGATTTGAATTTGAGTCCTTGCCCTTTTACGGATCCAGTAACAGGTTTGCCCACTTGGCATGATAGGCCTCCATCTCCGCTTCTTTTGTATGGATTTAGCAAAGAAATAGTCGAGTGCCCTGGTTATTGGCCATCAAATGTTCAGGTTTGTGGCTTTTGGTTTCTCCCAAATGAATGGCAGTTTTCGTGCAAGCAGTGCAGTGAACTTTCCAGAATTGTTTCTTCAGAGCGTCTTTACACAAAAGATGAAATGTGTTCAGCTCATGTTCAGTTAGAATATTTTTTGAAGACCCCAGTGTCTATGCCACCTATTTTCATAGGCCTGAGCTCAATTGGAAGCATGGGATTTTTAAAAAACGCTCAAGCGTTTCTTCGTCTTCTCCAAACAGTCTTAGAGATAACAAATTACCGCTTTATCCTCTTTACAGCCGGCTACATACCTTTAGATGCAGCAATCCGAGCAGTTGCCGCTGAGTCATCACCATATGTAACCCACAAGCAGTTTAATGACGAGTGCATTTCTCTCTTCAATGACCGACTCTTTTGCTTTTCTGGAACTGTACCGTACAAGTGGCTGTTTACTCGATGTGCTGCTGCAATTCATCATGGGGGAAGTGGCACTACTGCTGCAGCACTATTGGCAGGAATCCCACAGGTAGTATGTCCATTTGTGCTAGATCAGTTTTACTGGGCTGAAAGAATGTTTTGGCTTGGAGTAGCACCCGAGCCATTGAAAAGAATCCATTTACTTCCAGAAGAAAGTGAAGAGAAAATAGCGGCTAATTTTCTATCGAGGGCCATACATGATGCATTATCCCCTGAAATTAAAGAACATGCTGTAGAAGTTTCTAAGAAAATCTCTCTTGAGGATGGTGTCTCAAACGCGGTCAAATATATCAAGGAAGAGATCATTGGTGGTTCAAGCTCGATCATTCGCAATCTCTTGCACTGGCCTTCGGACATAGTTTTCAGCAATGCGGACAGTCGAGATTCTCAGCAATGCGGACAGTCGAGATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

554

Amino Acids

62.37

Weight (kDa)

5.46

Isoelectric Point (pI)

49.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014580)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G24750
fragaria_vesca FvH4_5g30191 FvH4_5g30191
malus_domestica MD08G1224800.v1.1
prunus_persica Prupe.1G562100_v2.0.a1
pyrus_communis pycom08g19590
rosa_chinensis RchiOBHm_Chr7g0224451
rosa_laevigata RLG00000001894
rosa_multiflora Rmu_sc0003894.1_g000001
rosa_roxburghii Rroxscaffold_3G00234750
rosa_rugosa Rorug07G0223300 Rorug07G0223400
rosa_samantha Rh7AG379900 Rh7BG359500 Rh7CG399400 Rh7DG369100
rosa_wichuraiana Rw7G031180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 937
AatII GACGTC 1 cut(s) 162
Acc36I ACCTGC 1 cut(s) 73
Acc65I GGTACC 1 cut(s) 89
AccB1I GGYRCC 1 cut(s) 89
AccII CGCG 1 cut(s) 1526
AciI CCGC 9 cut(s) 125, 128, 719, 1051, 1110, 1418, 1526, 1619, 1644
AclWI GGATC 6 cut(s) 373, 583, 621, 634, 668, 681
AcoI YGGCCR 3 cut(s) 207, 602, 770
AcsI RAATTY 1 cut(s) 649
AcuI CTGAAG 1 cut(s) 849
AcyI GRCGYC 1 cut(s) 159
AfaI GTAC 5 cut(s) 91, 155, 280, 1203, 1208
AfiI CCNNNNNNNGG 3 cut(s) 379, 670, 683
AgsI TTSAA 7 cut(s) 47, 518, 649, 928, 1171, 1375, 1563
AjnI CCWGG 3 cut(s) 289, 629, 760
AjuI GAANNNNNNNTTGG 2 cut(s) 1536, 1568
AluBI AGCT 4 cut(s) 557, 902, 969, 1566
AluI AGCT 4 cut(s) 557, 902, 969, 1566
Alw21I GWGCWC 2 cut(s) 493, 971
Alw26I GTCTC 2 cut(s) 347, 1522
Alw44I GTGCAC 1 cut(s) 489
AlwI GGATC 6 cut(s) 373, 583, 621, 634, 668, 681
Ama87I CYCGRG 2 cut(s) 373, 1364
AoxI GGCC 9 cut(s) 81, 207, 370, 602, 707, 770, 961, 1437, 1592
ApaLI GTGCAC 1 cut(s) 489
ApoI RAATTY 1 cut(s) 649
ArsI GACNNNNNNTTYG 2 cut(s) 735, 767
Asp700I GAANNNNTTC 2 cut(s) 441, 859
Asp718I GGTACC 1 cut(s) 89
AspLEI GCGC 2 cut(s) 184, 510
AspS9I GGNCC 2 cut(s) 371, 1437
AsuHPI GGTGA 3 cut(s) 234, 398, 1113
AvaI CYCGRG 2 cut(s) 373, 1364
BaeGI GKGCMC 2 cut(s) 493, 761
BalI TGGCCA 2 cut(s) 604, 772
BamHI GGATCC 2 cut(s) 626, 673
BanI GGYRCC 1 cut(s) 89
BanII GRGCYC 1 cut(s) 971
BarI GAAGNNNNNNTAC 2 cut(s) 1246, 1278
BbsI GAAGAC 2 cut(s) 935, 1010
Bbv12I GWGCWC 2 cut(s) 493, 971
BccI CCATC 5 cut(s) 270, 417, 721, 781, 1505
BcgI CGANNNNNNTGC 4 cut(s) 480, 514, 1167, 1201
BciT130I CCWGG 3 cut(s) 291, 631, 762
BclI TGATCA 1 cut(s) 399
BcoDI GTCTC 2 cut(s) 347, 1522
BfaI CTAG 2 cut(s) 18, 1316
BfmI CTRYAG 3 cut(s) 548, 1269, 1479
BfoI RGCGCY 1 cut(s) 511
BfuAI ACCTGC 1 cut(s) 73
BglI GCCNNNNNGGC 2 cut(s) 125, 697
Bme1390I CCNGG 3 cut(s) 291, 631, 762
BmeT110I CYCGRG 2 cut(s) 373, 1364
BmgT120I GGNCC 2 cut(s) 371, 1437
BmiI GGNNCC 3 cut(s) 91, 628, 675
BmrFI CCNGG 3 cut(s) 291, 631, 762
BmrI ACTGGG 2 cut(s) 929, 1339
BmsI GCATC 2 cut(s) 1078, 1438
BmuI ACTGGG 2 cut(s) 929, 1339
BpiI GAAGAC 2 cut(s) 935, 1010
Bpu10I CCTNAGC 1 cut(s) 965
BpuEI CTTGAG 2 cut(s) 987, 1526
BsaBI GATNNNNATC 1 cut(s) 105
BsaHI GRCGYC 1 cut(s) 159
BsaJI CCNNGG 2 cut(s) 93, 760
Bsc4I CCNNNNNNNGG 3 cut(s) 379, 670, 683
Bse118I RCCGGY 1 cut(s) 1070
Bse1I ACTGG 6 cut(s) 151, 599, 677, 935, 1334, 1595
Bse3DI GCAATG 3 cut(s) 120, 1621, 1646
Bse8I GATNNNNATC 1 cut(s) 105
BseBI CCWGG 3 cut(s) 291, 631, 762
BseDI CCNNGG 2 cut(s) 93, 760
BseGI GGATG 3 cut(s) 428, 603, 1516
BseJI GATNNNNATC 1 cut(s) 105
BseLI CCNNNNNNNGG 3 cut(s) 379, 670, 683
BseMI GCAATG 3 cut(s) 120, 1621, 1646
BseMII CTCAG 3 cut(s) 956, 1104, 1649
BseNI ACTGG 6 cut(s) 151, 599, 677, 935, 1334, 1595
BseSI GKGCMC 2 cut(s) 493, 761
BsgI GTGCAG 1 cut(s) 859
Bsh1236I CGCG 1 cut(s) 1526
BshFI GGCC 9 cut(s) 83, 209, 372, 604, 709, 772, 963, 1439, 1594
BshNI GGYRCC 1 cut(s) 89
BsiHKAI GWGCWC 2 cut(s) 493, 971
BsiHKCI CYCGRG 2 cut(s) 373, 1364
BsiSI CCGG 1 cut(s) 1071
BslFI GGGAC 1 cut(s) 239
BslI CCNNNNNNNGG 3 cut(s) 379, 670, 683
BsmAI GTCTC 2 cut(s) 347, 1522
BsmFI GGGAC 1 cut(s) 239
BsmI GAATGC 1 cut(s) 175
BsnI GGCC 9 cut(s) 83, 209, 372, 604, 709, 772, 963, 1439, 1594
BsoBI CYCGRG 2 cut(s) 373, 1364
Bsp1286I GDGCHC 3 cut(s) 493, 761, 971
Bsp143I GATC 8 cut(s) 365, 399, 588, 626, 673, 1318, 1548, 1569
BspACI CCGC 9 cut(s) 125, 128, 719, 1051, 1110, 1418, 1526, 1619, 1644
BspANI GGCC 9 cut(s) 83, 209, 372, 604, 709, 772, 963, 1439, 1594
BspCNI CTCAG 3 cut(s) 957, 1105, 1648
BspFNI CGCG 1 cut(s) 1526
BspLI GGNNCC 3 cut(s) 91, 628, 675
BspMAI CTGCAG 1 cut(s) 1273
BspMI ACCTGC 1 cut(s) 73
BspPI GGATC 6 cut(s) 373, 583, 621, 634, 668, 681
BspT107I GGYRCC 1 cut(s) 89
BsrDI GCAATG 3 cut(s) 120, 1621, 1646
BsrFI RCCGGY 1 cut(s) 1070
BsrI ACTGG 6 cut(s) 151, 599, 677, 935, 1334, 1595
BssAI RCCGGY 1 cut(s) 1070
BssECI CCNNGG 2 cut(s) 93, 760
BssMI GATC 8 cut(s) 365, 399, 588, 626, 673, 1318, 1548, 1569
BssNI GRCGYC 1 cut(s) 159
BssT1I CCWWGG 1 cut(s) 93
Bst2UI CCWGG 3 cut(s) 291, 631, 762
Bst4CI ACNGT 5 cut(s) 1030, 1201, 1206, 1625, 1650
Bst6I CTCTTC 3 cut(s) 1172, 1401, 1539
BstACI GRCGYC 1 cut(s) 159
BstAPI GCANNNNNTGC 1 cut(s) 837
BstC8I GCNNGC 3 cut(s) 23, 833, 1072
BstDEI CTNAG 6 cut(s) 567, 965, 1033, 1113, 1491, 1635
BstF5I GGATG 3 cut(s) 428, 603, 1516
BstFNI CGCG 1 cut(s) 1526
BstH2I RGCGCY 1 cut(s) 511
BstHHI GCGC 2 cut(s) 184, 510
BstKTI GATC 8 cut(s) 368, 402, 591, 629, 676, 1321, 1551, 1572
BstMAI GTCTC 2 cut(s) 347, 1522
BstMBI GATC 8 cut(s) 365, 399, 588, 626, 673, 1318, 1548, 1569
BstNI CCWGG 3 cut(s) 291, 631, 762
BstNSI RCATGY 2 cut(s) 221, 1478
BstSCI CCNGG 3 cut(s) 289, 629, 760
BstSFI CTRYAG 3 cut(s) 548, 1269, 1479
BstSLI GKGCMC 2 cut(s) 493, 761
BstUI CGCG 1 cut(s) 1526
BstV2I GAAGAC 2 cut(s) 935, 1010
BstX2I RGATCY 2 cut(s) 626, 673
BstXI CCANNNNNNTGG 1 cut(s) 816
BstYI RGATCY 2 cut(s) 626, 673
BsuRI GGCC 9 cut(s) 83, 209, 372, 604, 709, 772, 963, 1439, 1594
BtgZI GCGATG 2 cut(s) 114, 649
BtsCI GGATG 3 cut(s) 428, 603, 1516
BtsI GCAGTG 3 cut(s) 354, 842, 847
BtsIMutI CAGTG 6 cut(s) 354, 592, 842, 847, 942, 1588
BveI ACCTGC 1 cut(s) 73
Cac8I GCNNGC 3 cut(s) 23, 833, 1072
CfoI GCGC 2 cut(s) 184, 510
Cfr10I RCCGGY 1 cut(s) 1070
Cfr13I GGNCC 2 cut(s) 371, 1437
CseI GACGC 1 cut(s) 860
CsiI ACCWGGT 1 cut(s) 289
Csp6I GTAC 5 cut(s) 90, 154, 279, 1202, 1207
CviQI GTAC 5 cut(s) 90, 154, 279, 1202, 1207
DdeI CTNAG 6 cut(s) 567, 965, 1033, 1113, 1491, 1635
DpnI GATC 8 cut(s) 367, 401, 590, 628, 675, 1320, 1550, 1571
DpnII GATC 8 cut(s) 365, 399, 588, 626, 673, 1318, 1548, 1569
DraI TTTAAA 1 cut(s) 993
DrdI GACNNNNNNGTC 1 cut(s) 937
DseDI GACNNNNNNGTC 1 cut(s) 937
EaeI YGGCCR 3 cut(s) 207, 602, 770
Eam1104I CTCTTC 3 cut(s) 1172, 1401, 1539
EarI CTCTTC 3 cut(s) 1172, 1401, 1539
Ecl136II GAGCTC 1 cut(s) 969
Eco130I CCWWGG 1 cut(s) 93
Eco147I AGGCCT 2 cut(s) 709, 963
Eco24I GRGCYC 1 cut(s) 971
Eco53kI GAGCTC 1 cut(s) 969
Eco57I CTGAAG 1 cut(s) 849
Eco88I CYCGRG 2 cut(s) 373, 1364
EcoICRI GAGCTC 1 cut(s) 969
EcoRII CCWGG 3 cut(s) 289, 629, 760
EcoT14I CCWWGG 1 cut(s) 93
EcoT22I ATGCAT 1 cut(s) 1453
EcoT38I GRGCYC 1 cut(s) 971
ErhI CCWWGG 1 cut(s) 93
FalI AAGNNNNNCTT 2 cut(s) 996, 1028
FaqI GGGAC 1 cut(s) 239
FauNDI CATATG 1 cut(s) 1126
FbaI TGATCA 1 cut(s) 399
FokI GGATG 3 cut(s) 435, 610, 1523
FriOI GRGCYC 1 cut(s) 971
FspBI CTAG 2 cut(s) 18, 1316
GlaI GCGC 2 cut(s) 183, 509
HaeII RGCGCY 1 cut(s) 511
HaeIII GGCC 9 cut(s) 83, 209, 372, 604, 709, 772, 963, 1439, 1594
HapII CCGG 1 cut(s) 1071
HgaI GACGC 1 cut(s) 860
HhaI GCGC 2 cut(s) 184, 510
Hin1I GRCGYC 1 cut(s) 159
Hin6I GCGC 2 cut(s) 182, 508
HinP1I GCGC 2 cut(s) 182, 508
HinfI GANTC 7 cut(s) 655, 1115, 1179, 1287, 1380, 1631, 1656
HpaII CCGG 1 cut(s) 1071
HphI GGTGA 3 cut(s) 234, 398, 1113
Hpy166II GTNNAC 3 cut(s) 491, 845, 1221
Hpy188I TCNGA 4 cut(s) 868, 1100, 1600, 1661
Hpy188III TCNNGA 7 cut(s) 266, 852, 1194, 1394, 1505, 1628, 1653
Hpy8I GTNNAC 3 cut(s) 491, 845, 1221
Hpy99I CGWCG 3 cut(s) 161, 164, 502
HpyAV CCTTC 4 cut(s) 94, 413, 504, 1605
HpyCH4III ACNGT 5 cut(s) 1030, 1201, 1206, 1625, 1650
HpyCH4IV ACGT 2 cut(s) 159, 196
HpyF3I CTNAG 6 cut(s) 567, 965, 1033, 1113, 1491, 1635
HpySE526I ACGT 2 cut(s) 159, 196
Hsp92I GRCGYC 1 cut(s) 159
HspAI GCGC 2 cut(s) 182, 508
KpnI GGTACC 1 cut(s) 93
KroI GCCGGC 1 cut(s) 1070
KroNI GCCGGC 1 cut(s) 1072
Ksp22I TGATCA 1 cut(s) 399
Kzo9I GATC 8 cut(s) 365, 399, 588, 626, 673, 1318, 1548, 1569
LmnI GCTCC 1 cut(s) 472
LweI GCATC 2 cut(s) 1078, 1438
MabI ACCWGGT 1 cut(s) 289
MaeI CTAG 2 cut(s) 18, 1316
MaeII ACGT 2 cut(s) 159, 196
MaeIII GTNAC 4 cut(s) 140, 638, 679, 1129
MalI GATC 8 cut(s) 367, 401, 590, 628, 675, 1320, 1550, 1571
MboI GATC 8 cut(s) 365, 399, 588, 626, 673, 1318, 1548, 1569
MfeI CAATTG 2 cut(s) 111, 972
MflI RGATCY 2 cut(s) 626, 673
MhlI GDGCHC 3 cut(s) 493, 761, 971
MlsI TGGCCA 2 cut(s) 604, 772
MluNI TGGCCA 2 cut(s) 604, 772
MlyI GAGTC 3 cut(s) 664, 1124, 1173
MmeI TCCRAC 1 cut(s) 557
MnlI CCTC 8 cut(s) 34, 48, 176, 376, 720, 1070, 1428, 1501
Mox20I TGGCCA 2 cut(s) 604, 772
Mph1103I ATGCAT 1 cut(s) 1453
MroNI GCCGGC 1 cut(s) 1070
MroXI GAANNNNTTC 2 cut(s) 441, 859
MscI TGGCCA 2 cut(s) 604, 772
MseI TTAA 3 cut(s) 992, 1146, 1467
Msp20I TGGCCA 2 cut(s) 604, 772
MspA1I CMGCKG 1 cut(s) 1112
MspI CCGG 1 cut(s) 1071
MspR9I CCNGG 3 cut(s) 291, 631, 762
MunI CAATTG 2 cut(s) 111, 972
Mva1269I GAATGC 1 cut(s) 175
MvaI CCWGG 3 cut(s) 291, 631, 762
MvnI CGCG 1 cut(s) 1526
NaeI GCCGGC 1 cut(s) 1072
NdeI CATATG 1 cut(s) 1126
NdeII GATC 8 cut(s) 365, 399, 588, 626, 673, 1318, 1548, 1569
NgoMIV GCCGGC 1 cut(s) 1070
NlaIV GGNNCC 3 cut(s) 91, 628, 675
NmuCI GTSAC 2 cut(s) 140, 638
NsiI ATGCAT 1 cut(s) 1453
NspI RCATGY 2 cut(s) 221, 1478
PceI AGGCCT 2 cut(s) 709, 963
PcsI WCGNNNNNNNCGW 1 cut(s) 56
PctI GAATGC 1 cut(s) 175
PdiI GCCGGC 1 cut(s) 1072
PdmI GAANNNNTTC 2 cut(s) 441, 859
PfeI GAWTC 4 cut(s) 1287, 1380, 1631, 1656
PflFI GACNNNGTC 1 cut(s) 161
PleI GAGTC 3 cut(s) 663, 1123, 1173
PpsI GAGTC 3 cut(s) 663, 1123, 1173
Psp124BI GAGCTC 1 cut(s) 971
Psp6I CCWGG 3 cut(s) 289, 629, 760
PspGI CCWGG 3 cut(s) 289, 629, 760
PspN4I GGNNCC 3 cut(s) 91, 628, 675
PspPI GGNCC 2 cut(s) 371, 1437
PstI CTGCAG 1 cut(s) 1273
PsuI RGATCY 2 cut(s) 626, 673
PsyI GACNNNGTC 1 cut(s) 161
RsaI GTAC 5 cut(s) 91, 155, 280, 1203, 1208
RsaNI GTAC 5 cut(s) 90, 154, 279, 1202, 1207
SacI GAGCTC 1 cut(s) 971
SaqAI TTAA 3 cut(s) 992, 1146, 1467
Sau3AI GATC 8 cut(s) 365, 399, 588, 626, 673, 1318, 1548, 1569
Sau96I GGNCC 2 cut(s) 371, 1437
SchI GAGTC 3 cut(s) 664, 1124, 1173
ScrFI CCNGG 3 cut(s) 291, 631, 762
SduI GDGCHC 3 cut(s) 493, 761, 971
SexAI ACCWGGT 1 cut(s) 289
SfaNI GCATC 2 cut(s) 1078, 1438
SfcI CTRYAG 3 cut(s) 548, 1269, 1479
SmlI CTYRAG 2 cut(s) 1002, 1505
SmoI CTYRAG 2 cut(s) 1002, 1505
SseBI AGGCCT 2 cut(s) 709, 963
SsiI CCGC 9 cut(s) 125, 128, 719, 1051, 1110, 1418, 1526, 1619, 1644
SspI AATATT 2 cut(s) 360, 920
SspMI CTAG 2 cut(s) 18, 1316
SstI GAGCTC 1 cut(s) 971
StuI AGGCCT 2 cut(s) 709, 963
StyD4I CCNGG 3 cut(s) 289, 629, 760
StyI CCWWGG 1 cut(s) 93
TaaI ACNGT 5 cut(s) 1030, 1201, 1206, 1625, 1650
TaiI ACGT 2 cut(s) 162, 199
TaqI TCGA 6 cut(s) 753, 1225, 1433, 1568, 1627, 1652
TaqII GACCGA 1 cut(s) 1191
TatI WGTACW 1 cut(s) 278
TauI GCSGC 4 cut(s) 128, 131, 1112, 1421
TfiI GAWTC 4 cut(s) 1287, 1380, 1631, 1656
Tru1I TTAA 3 cut(s) 992, 1146, 1467
Tru9I TTAA 3 cut(s) 992, 1146, 1467
TscAI CASTG 6 cut(s) 354, 599, 842, 847, 942, 1595
TseFI GTSAC 2 cut(s) 140, 638
Tsp45I GTSAC 2 cut(s) 140, 638
TspDTI ATGAA 7 cut(s) 67, 228, 434, 828, 903, 946, 1232
TspGWI ACGGA 3 cut(s) 65, 440, 686
TspRI CASTG 6 cut(s) 354, 599, 842, 847, 942, 1595
Tth111I GACNNNGTC 1 cut(s) 161
VneI GTGCAC 1 cut(s) 489
XapI RAATTY 1 cut(s) 649
XceI RCATGY 2 cut(s) 221, 1478
XmnI GAANNNNTTC 2 cut(s) 441, 859
XspI CTAG 2 cut(s) 18, 1316
ZraI GACGTC 1 cut(s) 160
Zsp2I ATGCAT 1 cut(s) 1453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.