Rorug07G0223300

Sterol 3-beta-glucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0014580
Physical Location & Seq
Reverse (-)
20302748 .. 20309165
6418 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0223300.1

Sequence Viewer

Length: 1149 bp
ATGGCAGCAGCCTCTTCTCCCTCCCCAGCCCTAAGCGCCACCACCTCCGACGCCTCTCATGCCGCCACCGCCGCCGTCCCCGCCCCGCCCTTCTCCGCGGCTTTCCGCTCCGAAACTCCGACCAAGACGCTCCGCGGCCTCAACAAGCCCAAGTGCAAGGTCTGCGGCAATGTCGCTCGCTCCAGGTGTCCGTTTGAGTCTTGCAAGAGTTGCTGCTCAAGAGCTCAAAATCCATGTCACATTCATGTCTTGAAAGCAAATACAACTATTCCAGACAAGGCACCAGCCTCAAGCAGTCCTCAATTTGACCATCAGTCAACTGAGACATCATCCTCCGGGAATTCGCATAGAGTTGCATCACTCCGACAACTTTCGAACAATTTTGCGCAATTCAATAATGTGCATATTACACTTCGCACAAAGAAGCCGCTGACCAGAAAGGATGCTGTGGCTACTAATGAATGGAGGTTTGCCAAGTTAAAGGAATTTAAGGACAGAAACATTGAAGTGGAAAATGAAGCTTTTGATCGATACATGCAGAACATCAGCTTATTAGGGGAAGTGTTTTCTGTGAACTCTAATGCGGAAGAGTCCATCAGGGATGGATCATCCATATCAAACCATGACAGGACTACTACAGAAGGTGACAATGGGGATATGGTCTCAGGGTTGAAGTTAAAGTTGAGATCAAATCCAATGAGAACCAGTAATTTCAGAAAGAGGATACAGCAAATTGTTGATCAGGGACTAAAGAAGCTTAAAACATGTGAGTCTGCTGATGGAGATAAGGAACCGAGTGACCAAATTGAATTGGATATGGGGCCCAGAAAAGGAGAAAATTGGCGGGCTGCAAGGGCTTCAGCTTTAGGTGATCTCATTGATAAGTTGAACAAAGCCCGAAATGAGGAGGATCTACAGTCTTGCATGGAGATGAAGACTCAACTCTTCAATGAACTGAAATCTATGAGCAGCTCAGAATTGGAAGACACAGAGACCACGAAGAAACAGACTACGAAGAATGACTTGGAAACCAGGAAAGAGTCGCAATATTTATTTCCTCATTTGATTAGTACCAATGAAATTGATCAAGAAACTCTCAGTAGCGTCAATGCGCACTTCTCTTCCCTGGAGCAGATAGAAGGTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

382

Amino Acids

42.3

Weight (kDa)

8.02

Isoelectric Point (pI)

52.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF702 PF05142 51 - 114 9e-06 Domain of unknown function (DUF702)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014580)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G24750
fragaria_vesca FvH4_5g30191 FvH4_5g30191
malus_domestica MD08G1224800.v1.1
prunus_persica Prupe.1G562100_v2.0.a1
pyrus_communis pycom08g19590
rosa_chinensis RchiOBHm_Chr7g0224451
rosa_laevigata RLG00000001894
rosa_multiflora Rmu_sc0003894.1_g000001
rosa_roxburghii Rroxscaffold_3G00234750
rosa_rugosa Rorug07G0223300 Rorug07G0223400
rosa_samantha Rh7AG379900 Rh7BG359500 Rh7CG399400 Rh7DG369100
rosa_wichuraiana Rw7G031180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 387, 1113
AccB1I GGYRCC 1 cut(s) 280
AccBSI CCGCTC 1 cut(s) 108
AccII CGCG 2 cut(s) 98, 135
AclWI GGATC 2 cut(s) 613, 918
AcsI RAATTY 2 cut(s) 340, 485
AcuI CTGAAG 1 cut(s) 843
AcyI GRCGYC 1 cut(s) 51
AfaI GTAC 1 cut(s) 1072
AfiI CCNNNNNNNGG 2 cut(s) 830, 904
AflIII ACRYGT 1 cut(s) 764
AgsI TTSAA 7 cut(s) 253, 394, 506, 673, 809, 889, 949
AhdI GACNNNNNGTC 1 cut(s) 313
AjnI CCWGG 3 cut(s) 182, 1031, 1125
AjuI GAANNNNNNNTTGG 2 cut(s) 1007, 1039
AluBI AGCT 6 cut(s) 224, 521, 549, 757, 863, 972
AluI AGCT 6 cut(s) 224, 521, 549, 757, 863, 972
Alw21I GWGCWC 1 cut(s) 226
Alw26I GTCTC 3 cut(s) 317, 667, 986
AlwI GGATC 2 cut(s) 613, 918
AoxI GGCC 2 cut(s) 136, 821
ApaI GGGCCC 1 cut(s) 825
ApeKI GCWGC 5 cut(s) 5, 8, 213, 848, 969
ApoI RAATTY 2 cut(s) 340, 485
ArsI GACNNNNNNTTYG 2 cut(s) 220, 252
AspLEI GCGC 3 cut(s) 38, 388, 1114
AspS9I GGNCC 2 cut(s) 821, 822
AsuC2I CCSGG 1 cut(s) 337
AsuHPI GGTGA 2 cut(s) 656, 881
AsuII TTCGAA 1 cut(s) 374
BaeGI GKGCMC 1 cut(s) 825
BanI GGYRCC 1 cut(s) 280
BanII GRGCYC 2 cut(s) 226, 825
BbsI GAAGAC 2 cut(s) 941, 990
Bbv12I GWGCWC 1 cut(s) 226
BbvI GCAGC 5 cut(s) 17, 20, 200, 835, 981
BccI CCATC 4 cut(s) 318, 596, 602, 773
BceAI ACGGC 1 cut(s) 59
BciT130I CCWGG 3 cut(s) 184, 1033, 1127
BciVI GTATCC 1 cut(s) 717
BclI TGATCA 2 cut(s) 739, 1084
BcnI CCSGG 1 cut(s) 337
BcoDI GTCTC 3 cut(s) 317, 667, 986
BfmI CTRYAG 2 cut(s) 636, 914
BfoI RGCGCY 1 cut(s) 39
BfuI GTATCC 1 cut(s) 717
Bme1390I CCNGG 4 cut(s) 184, 337, 1033, 1127
BmeRI GACNNNNNGTC 1 cut(s) 313
BmgT120I GGNCC 2 cut(s) 821, 822
BmiI GGNNCC 4 cut(s) 282, 792, 822, 823
BmrFI CCNGG 4 cut(s) 184, 337, 1033, 1127
BmsI GCATC 2 cut(s) 365, 433
BpiI GAAGAC 2 cut(s) 941, 990
BpmI CTGGAG 2 cut(s) 166, 1148
Bpu10I CCTNAGC 1 cut(s) 32
Bpu14I TTCGAA 1 cut(s) 374
BpuEI CTTGAG 2 cut(s) 202, 274
BpuMI CCSGG 1 cut(s) 337
Bsa29I ATCGAT 1 cut(s) 529
BsaHI GRCGYC 1 cut(s) 51
BsaI GGTCTC 2 cut(s) 667, 986
BsaJI CCNNGG 3 cut(s) 96, 133, 1125
Bsc4I CCNNNNNNNGG 2 cut(s) 830, 904
Bse1I ACTGG 1 cut(s) 705
Bse3DI GCAATG 1 cut(s) 175
BseBI CCWGG 3 cut(s) 184, 1033, 1127
BseCI ATCGAT 1 cut(s) 529
BseDI CCNNGG 3 cut(s) 96, 133, 1125
BseGI GGATG 4 cut(s) 329, 448, 607, 608
BseLI CCNNNNNNNGG 2 cut(s) 830, 904
BseMI GCAATG 1 cut(s) 175
BseMII CTCAG 4 cut(s) 312, 678, 987, 1111
BseNI ACTGG 1 cut(s) 705
BseRI GAGGAG 1 cut(s) 920
BseSI GKGCMC 1 cut(s) 825
BseXI GCAGC 5 cut(s) 17, 20, 200, 835, 981
BseYI CCCAGC 1 cut(s) 25
Bsh1236I CGCG 2 cut(s) 98, 135
BshFI GGCC 2 cut(s) 138, 823
BshNI GGYRCC 1 cut(s) 280
BshVI ATCGAT 1 cut(s) 529
BsiHKAI GWGCWC 1 cut(s) 226
BsiSI CCGG 1 cut(s) 336
BslFI GGGAC 2 cut(s) 62, 759
BslI CCNNNNNNNGG 2 cut(s) 830, 904
BsmAI GTCTC 3 cut(s) 317, 667, 986
BsmFI GGGAC 2 cut(s) 62, 759
BsnI GGCC 2 cut(s) 138, 823
Bso31I GGTCTC 2 cut(s) 667, 986
Bsp119I TTCGAA 1 cut(s) 374
Bsp120I GGGCCC 1 cut(s) 821
Bsp1286I GDGCHC 2 cut(s) 226, 825
Bsp143I GATC 7 cut(s) 526, 605, 686, 739, 871, 910, 1084
BspANI GGCC 2 cut(s) 138, 823
BspCNI CTCAG 4 cut(s) 313, 677, 986, 1110
BspDI ATCGAT 1 cut(s) 529
BspFNI CGCG 2 cut(s) 98, 135
BspLI GGNNCC 4 cut(s) 282, 792, 822, 823
BspPI GGATC 2 cut(s) 613, 918
BspT104I TTCGAA 1 cut(s) 374
BspT107I GGYRCC 1 cut(s) 280
BspTNI GGTCTC 2 cut(s) 667, 986
BsrBI CCGCTC 1 cut(s) 108
BsrDI GCAATG 1 cut(s) 175
BsrI ACTGG 1 cut(s) 705
BssECI CCNNGG 3 cut(s) 96, 133, 1125
BssMI GATC 7 cut(s) 526, 605, 686, 739, 871, 910, 1084
BssNI GRCGYC 1 cut(s) 51
Bst2UI CCWGG 3 cut(s) 184, 1033, 1127
Bst4CI ACNGT 1 cut(s) 918
Bst6I CTCTTC 4 cut(s) 19, 582, 950, 1126
BstACI GRCGYC 1 cut(s) 51
BstAPI GCANNNNNTGC 2 cut(s) 162, 210
BstBI TTCGAA 1 cut(s) 374
BstC8I GCNNGC 2 cut(s) 178, 846
BstDEI CTNAG 5 cut(s) 32, 321, 664, 973, 1097
BstDSI CCRYGG 2 cut(s) 96, 133
BstF5I GGATG 4 cut(s) 329, 448, 607, 608
BstFNI CGCG 2 cut(s) 98, 135
BstH2I RGCGCY 1 cut(s) 39
BstHHI GCGC 3 cut(s) 38, 388, 1114
BstKTI GATC 7 cut(s) 529, 608, 689, 742, 874, 913, 1087
BstMAI GTCTC 3 cut(s) 317, 667, 986
BstMBI GATC 7 cut(s) 526, 605, 686, 739, 871, 910, 1084
BstMWI GCNNNNNNNGC 8 cut(s) 35, 59, 68, 71, 80, 162, 210, 854
BstNI CCWGG 3 cut(s) 184, 1033, 1127
BstNSI RCATGY 2 cut(s) 538, 768
BstSCI CCNGG 4 cut(s) 182, 335, 1031, 1125
BstSFI CTRYAG 2 cut(s) 636, 914
BstSLI GKGCMC 1 cut(s) 825
BstUI CGCG 2 cut(s) 98, 135
BstV1I GCAGC 5 cut(s) 17, 20, 200, 835, 981
BstV2I GAAGAC 2 cut(s) 941, 990
BstX2I RGATCY 1 cut(s) 910
BstYI RGATCY 1 cut(s) 910
Bsu15I ATCGAT 1 cut(s) 529
BsuI GTATCC 1 cut(s) 717
BsuRI GGCC 2 cut(s) 138, 823
BsuTUI ATCGAT 1 cut(s) 529
BtgI CCRYGG 2 cut(s) 96, 133
BtsCI GGATG 4 cut(s) 329, 448, 607, 608
Cac8I GCNNGC 2 cut(s) 178, 846
CfoI GCGC 3 cut(s) 38, 388, 1114
Cfr13I GGNCC 2 cut(s) 821, 822
Cfr42I CCGCGG 2 cut(s) 99, 136
ClaI ATCGAT 1 cut(s) 529
CseI GACGC 3 cut(s) 59, 136, 1093
Csp6I GTAC 1 cut(s) 1071
CviAII CATG 7 cut(s) 59, 234, 245, 535, 623, 765, 925
CviQI GTAC 1 cut(s) 1071
DdeI CTNAG 5 cut(s) 32, 321, 664, 973, 1097
DpnI GATC 7 cut(s) 528, 607, 688, 741, 873, 912, 1086
DpnII GATC 7 cut(s) 526, 605, 686, 739, 871, 910, 1084
DriI GACNNNNNGTC 1 cut(s) 313
Eam1104I CTCTTC 4 cut(s) 19, 582, 950, 1126
Eam1105I GACNNNNNGTC 1 cut(s) 313
EarI CTCTTC 4 cut(s) 19, 582, 950, 1126
Ecl136II GAGCTC 1 cut(s) 224
Eco24I GRGCYC 2 cut(s) 226, 825
Eco31I GGTCTC 2 cut(s) 667, 986
Eco53kI GAGCTC 1 cut(s) 224
Eco57I CTGAAG 1 cut(s) 843
EcoICRI GAGCTC 1 cut(s) 224
EcoO109I RGGNCCY 1 cut(s) 821
EcoRI GAATTC 1 cut(s) 340
EcoRII CCWGG 3 cut(s) 182, 1031, 1125
EcoT38I GRGCYC 2 cut(s) 226, 825
FaeI CATG 7 cut(s) 62, 237, 248, 538, 626, 768, 928
FalI AAGNNNNNCTT 2 cut(s) 1007, 1039
FaqI GGGAC 2 cut(s) 62, 759
FatI CATG 7 cut(s) 58, 233, 244, 534, 622, 764, 924
FauI CCCGC 3 cut(s) 88, 93, 837
FbaI TGATCA 2 cut(s) 739, 1084
FokI GGATG 4 cut(s) 316, 455, 595, 614
FriOI GRGCYC 2 cut(s) 226, 825
FspAI RTGCGCAY 1 cut(s) 1113
FspI TGCGCA 2 cut(s) 387, 1113
GlaI GCGC 3 cut(s) 37, 387, 1113
GsaI CCCAGC 1 cut(s) 29
GsuI CTGGAG 2 cut(s) 166, 1148
HaeII RGCGCY 1 cut(s) 39
HaeIII GGCC 2 cut(s) 138, 823
HapII CCGG 1 cut(s) 336
HgaI GACGC 3 cut(s) 59, 136, 1093
HhaI GCGC 3 cut(s) 38, 388, 1114
Hin1I GRCGYC 1 cut(s) 51
Hin1II CATG 7 cut(s) 62, 237, 248, 538, 626, 768, 928
Hin6I GCGC 3 cut(s) 36, 386, 1112
HinP1I GCGC 3 cut(s) 36, 386, 1112
HincII GTYRAC 1 cut(s) 318
HindII GTYRAC 1 cut(s) 318
HindIII AAGCTT 2 cut(s) 519, 755
HinfI GANTC 5 cut(s) 197, 590, 770, 937, 1040
HpaII CCGG 1 cut(s) 336
HphI GGTGA 2 cut(s) 656, 881
Hpy166II GTNNAC 2 cut(s) 318, 574
Hpy188I TCNGA 6 cut(s) 49, 112, 120, 365, 716, 976
Hpy188III TCNNGA 4 cut(s) 219, 250, 272, 1088
Hpy8I GTNNAC 2 cut(s) 318, 574
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 3 cut(s) 100, 635, 1133
HpyCH4III ACNGT 1 cut(s) 918
HpyCH4V TGCA 7 cut(s) 156, 204, 356, 403, 538, 851, 924
HpyF10VI GCNNNNNNNGC 8 cut(s) 35, 59, 68, 71, 80, 162, 210, 854
HpyF3I CTNAG 5 cut(s) 32, 321, 664, 973, 1097
Hsp92I GRCGYC 1 cut(s) 51
Hsp92II CATG 7 cut(s) 62, 237, 248, 538, 626, 768, 928
HspAI GCGC 3 cut(s) 36, 386, 1112
Ksp22I TGATCA 2 cut(s) 739, 1084
KspI CCGCGG 2 cut(s) 99, 136
Kzo9I GATC 7 cut(s) 526, 605, 686, 739, 871, 910, 1084
LmnI GCTCC 4 cut(s) 113, 135, 185, 1129
Lsp1109I GCAGC 5 cut(s) 17, 20, 200, 835, 981
LweI GCATC 2 cut(s) 365, 433
MaeIII GTNAC 3 cut(s) 236, 644, 797
MalI GATC 7 cut(s) 528, 607, 688, 741, 873, 912, 1086
MbiI CCGCTC 1 cut(s) 108
MboI GATC 7 cut(s) 526, 605, 686, 739, 871, 910, 1084
MboII GAAGA 8 cut(s) 6, 599, 937, 946, 995, 1012, 1027, 1113
MflI RGATCY 1 cut(s) 910
MhlI GDGCHC 2 cut(s) 226, 825
MlyI GAGTC 5 cut(s) 206, 599, 779, 931, 1049
MmeI TCCRAC 3 cut(s) 72, 143, 388
MseI TTAA 4 cut(s) 479, 489, 677, 759
MslI CAYNNNNRTG 3 cut(s) 243, 506, 929
MspA1I CMGCKG 3 cut(s) 98, 135, 430
MspI CCGG 1 cut(s) 336
MspR9I CCNGG 4 cut(s) 184, 337, 1033, 1127
MvaI CCWGG 3 cut(s) 184, 1033, 1127
MvnI CGCG 2 cut(s) 98, 135
MwoI GCNNNNNNNGC 8 cut(s) 35, 59, 68, 71, 80, 162, 210, 854
NciI CCSGG 1 cut(s) 337
NdeII GATC 7 cut(s) 526, 605, 686, 739, 871, 910, 1084
NlaIII CATG 7 cut(s) 62, 237, 248, 538, 626, 768, 928
NlaIV GGNNCC 4 cut(s) 282, 792, 822, 823
NmuCI GTSAC 3 cut(s) 236, 644, 797
NsbI TGCGCA 2 cut(s) 387, 1113
NspI RCATGY 2 cut(s) 538, 768
NspV TTCGAA 1 cut(s) 374
PciI ACATGT 1 cut(s) 764
PfoI TCCNGGA 1 cut(s) 335
PleI GAGTC 5 cut(s) 205, 598, 778, 931, 1048
PpsI GAGTC 5 cut(s) 205, 598, 778, 931, 1048
PscI ACATGT 1 cut(s) 764
Psp124BI GAGCTC 1 cut(s) 226
Psp6I CCWGG 3 cut(s) 182, 1031, 1125
PspFI CCCAGC 1 cut(s) 25
PspGI CCWGG 3 cut(s) 182, 1031, 1125
PspN4I GGNNCC 4 cut(s) 282, 792, 822, 823
PspOMI GGGCCC 1 cut(s) 821
PspPI GGNCC 2 cut(s) 821, 822
PsuI RGATCY 1 cut(s) 910
RsaI GTAC 1 cut(s) 1072
RsaNI GTAC 1 cut(s) 1071
RseI CAYNNNNRTG 3 cut(s) 243, 506, 929
SacI GAGCTC 1 cut(s) 226
SacII CCGCGG 2 cut(s) 99, 136
SaqAI TTAA 4 cut(s) 479, 489, 677, 759
Sau3AI GATC 7 cut(s) 526, 605, 686, 739, 871, 910, 1084
Sau96I GGNCC 2 cut(s) 821, 822
SchI GAGTC 5 cut(s) 206, 599, 779, 931, 1049
ScrFI CCNGG 4 cut(s) 184, 337, 1033, 1127
SduI GDGCHC 2 cut(s) 226, 825
SfaNI GCATC 2 cut(s) 365, 433
SfcI CTRYAG 2 cut(s) 636, 914
Sfr303I CCGCGG 2 cut(s) 99, 136
SfuI TTCGAA 1 cut(s) 374
SgrBI CCGCGG 2 cut(s) 99, 136
SmiMI CAYNNNNRTG 3 cut(s) 243, 506, 929
SmlI CTYRAG 2 cut(s) 217, 289
SmoI CTYRAG 2 cut(s) 217, 289
SspI AATATT 1 cut(s) 1049
SstI GAGCTC 1 cut(s) 226
StyD4I CCNGG 4 cut(s) 182, 335, 1031, 1125
TaaI ACNGT 1 cut(s) 918
TaqI TCGA 2 cut(s) 374, 529
TauI GCSGC 6 cut(s) 65, 74, 101, 138, 168, 430
Tru1I TTAA 4 cut(s) 479, 489, 677, 759
Tru9I TTAA 4 cut(s) 479, 489, 677, 759
TseFI GTSAC 3 cut(s) 236, 644, 797
TseI GCWGC 5 cut(s) 5, 8, 213, 848, 969
Tsp45I GTSAC 3 cut(s) 236, 644, 797
TspDTI ATGAA 6 cut(s) 233, 474, 531, 947, 966, 1092
TspGWI ACGGA 1 cut(s) 180
XapI RAATTY 2 cut(s) 340, 485
XceI RCATGY 2 cut(s) 538, 768
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.