Rh7BG359500

Sterol 3-beta-glucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0014580
Physical Location & Seq
Forward (+)
37900679 .. 37908698
8020 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG359500.1

Sequence Viewer

Length: 1548 bp
ATGGAGGTTGAGACGGAGAGGACGAAACCCGTTGCTGTATTCATGGCTTTCGGTACCAAAGGCGATGTCTATCCAATTGCTGCCATTGCGGCGGCCTTTGCTTCCGACCAGAGACAGTACCAAGTTGTTCTTATCACACATTCAGCTCACGAGAGCTTACGTTGTCACTTGGCTGAGAAGCATGTTTCCTATCTTCCAATTTCATCACCACCTGTTCTCTCTATCCATCAGAAACATGATACTACAGGCTCTGTAGAGTTGCCTCTTTCGCTGCAGAAAAAGTTGTTTTCTGGAGAACATAGAAAAGAATGTCTTTCGGTAGTTGAAACGATATTTGGATCAGGCCCAAGCATGGATGGTGATTTCTTTGTGATAAATTTCTTTGCTCTGGAGGGCTGGAGTCTTGCGGAGCTTTTCCGTGTCCGCTGCATGGTTGCTGCTCCTTATGTTGTTCCGTACAGTGCACCTTCATCATTTGAGCGCCAGTTTAAAAAGGAATATCAACACTTGTATAGATACCTGCAGGAACCTAACCTCAGGAAGGTTGGTTGGAAAGACGTGATCCACTGGATGTGGCCTCTTTTCACTGAACACTGGGGATCCTGGAGATGTGATCATTTGAATCTGAGTGCTTTCCCTTTTACAGATCCAGTAACGGGTCTCCCCACTTGGCATGAAAGGCCACTGTCTCCCCGACTTTTGTATGGTTTTAGCAAAGAAGTTGTTGAGTGCCCAGATTACTGGCCATCGAGCATTCGGGTTTGTGGCTTTTGGTTTCTGCCTAATGAATGGCAATTTTCATGTAAGAAGTGCAGTGAAATTTCAAGGCTTGTTTCTTCAGGGGATCTGTACACAAATGCTGAAATGTGCCCAGCTCATGTTCACTTGCAATATTTTCTGAAGACTCCTACGCCTATGCCACCTGTTTTTATTGGCCTGAATTCTATTGGTAGCATGGGATTTTTGAAGAATCCTCAAGCATTTCTTCGTATTCTTGAATCTGTCCTCAAGATAACAACTTATCGTTTTATCCTTTTTACTGCTGGCTACAAGCCTTTAGATGCAGCAATCCGAGCTATTGCTGCTGATTCATCATCGCACATGACCCACAAACAGATTAATGACGATTGCGTTTCTCTCTTCCACGAACGATTGTTCTGCTTTTCTGGTTCTATACCATACACGTGGCTGTTTTCTGGATGTGCGGCTGCAATTCATCATGGGGGAAGTGGAACTACTGCTGCAGCACTACTTGCAGGAATCCCACAGGTACTATGTCCATTTATGCTAGACCAGTTTTATTGGGCAGAAAGAATGTTTTGGCTTGGAGTGGCACCAGAACCGTTGAAAAGGAGCCACTTGCTTCCTGAAGAAAGTGATGATGAAAGTATCGAAGAAGCAGCCAAGATGCTATCAAGAGTCATACATGATACATTATCTCCTGAAATTAGAGAACGCGCTGTAGAAATTTCTGAGAGAATATCTCTTGAGGACGGAGTCTCAAATGCTGTTAGGTATATCAAGGAAGAGATTGGTTGTTCAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

515

Amino Acids

58.35

Weight (kDa)

6.16

Isoelectric Point (pI)

42.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EryCIII-like_C PF06722 391 - 445 3.3e-12 Erythromycin biosynthesis protein CIII-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014580)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G24750
fragaria_vesca FvH4_5g30191 FvH4_5g30191
malus_domestica MD08G1224800.v1.1
prunus_persica Prupe.1G562100_v2.0.a1
pyrus_communis pycom08g19590
rosa_chinensis RchiOBHm_Chr7g0224451
rosa_laevigata RLG00000001894
rosa_multiflora Rmu_sc0003894.1_g000001
rosa_roxburghii Rroxscaffold_3G00234750
rosa_rugosa Rorug07G0223300 Rorug07G0223400
rosa_samantha Rh7AG379900 Rh7BG359500 Rh7CG399400 Rh7DG369100
rosa_wichuraiana Rw7G031180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 528
Acc65I GGTACC 1 cut(s) 53
AccB1I GGYRCC 2 cut(s) 53, 1333
AccII CGCG 1 cut(s) 1458
AciI CCGC 5 cut(s) 89, 92, 407, 424, 1205
AclWI GGATC 6 cut(s) 346, 556, 594, 607, 641, 852
AcoI YGGCCR 1 cut(s) 743
AcsI RAATTY 4 cut(s) 376, 819, 940, 1467
AcuI CTGAAG 3 cut(s) 822, 920, 1389
AcvI CACGTG 1 cut(s) 1185
AfaI GTAC 5 cut(s) 55, 119, 458, 851, 1272
AfiI CCNNNNNNNGG 4 cut(s) 352, 430, 541, 656
AflIII ACRYGT 1 cut(s) 1182
AgsI TTSAA 7 cut(s) 326, 622, 825, 967, 998, 1348, 1542
AjiI CACGTC 1 cut(s) 559
AjnI CCWGG 1 cut(s) 602
AjuI GAANNNNNNNTTGG 2 cut(s) 318, 350
AluBI AGCT 6 cut(s) 146, 156, 412, 875, 1076, 1545
AluI AGCT 6 cut(s) 146, 156, 412, 875, 1076, 1545
Alw21I GWGCWC 1 cut(s) 466
Alw26I GTCTC 5 cut(s) 5, 106, 665, 693, 1504
Alw44I GTGCAC 1 cut(s) 462
AlwI GGATC 6 cut(s) 346, 556, 594, 607, 641, 852
AlwNI CAGNNNCTG 1 cut(s) 251
AoxI GGCC 6 cut(s) 93, 343, 575, 680, 743, 934
ApaLI GTGCAC 1 cut(s) 462
ApoI RAATTY 4 cut(s) 376, 819, 940, 1467
ArsI GACNNNNNNTTYG 2 cut(s) 51, 83
AseI ATTAAT 1 cut(s) 1119
Asp718I GGTACC 1 cut(s) 53
AspLEI GCGC 2 cut(s) 483, 1460
AspS9I GGNCC 1 cut(s) 344
AsuHPI GGTGA 2 cut(s) 198, 371
AxyI CCTNAGG 1 cut(s) 536
BaeGI GKGCMC 3 cut(s) 466, 734, 872
BalI TGGCCA 1 cut(s) 745
BamHI GGATCC 1 cut(s) 599
BanI GGYRCC 2 cut(s) 53, 1333
BarI GAAGNNNNNNTAC 2 cut(s) 1219, 1251
BauI CACGAG 1 cut(s) 149
BbrPI CACGTG 1 cut(s) 1185
BbsI GAAGAC 1 cut(s) 908
Bbv12I GWGCWC 1 cut(s) 466
BccI CCATC 3 cut(s) 234, 350, 754
BcgI CGANNNNNNTGC 2 cut(s) 1140, 1174
BciT130I CCWGG 1 cut(s) 604
BclI TGATCA 1 cut(s) 613
BcoDI GTCTC 5 cut(s) 5, 106, 665, 693, 1504
BfaI CTAG 1 cut(s) 1289
BfmI CTRYAG 6 cut(s) 243, 252, 272, 521, 1242, 1461
BfoI RGCGCY 1 cut(s) 484
BfuAI ACCTGC 1 cut(s) 528
BglI GCCNNNNNGGC 1 cut(s) 89
Bme1390I CCNGG 1 cut(s) 604
BmgBI CACGTC 1 cut(s) 559
BmgT120I GGNCC 1 cut(s) 344
BmiI GGNNCC 5 cut(s) 55, 528, 601, 1335, 1355
BmrFI CCNGG 1 cut(s) 604
BmrI ACTGGG 1 cut(s) 604
BmsI GCATC 2 cut(s) 1051, 1398
BmuI ACTGGG 1 cut(s) 604
BpiI GAAGAC 1 cut(s) 908
BplI GAGNNNNNCTC 2 cut(s) 1468, 1500
BpmI CTGGAG 4 cut(s) 312, 410, 418, 625
BpuEI CTTGAG 3 cut(s) 960, 992, 1508
BsaAI YACGTR 1 cut(s) 1185
BsaBI GATNNNNATC 1 cut(s) 69
BsaI GGTCTC 1 cut(s) 665
BsaXI ACNNNNNCTCC 2 cut(s) 1423, 1453
Bsc4I CCNNNNNNNGG 4 cut(s) 352, 430, 541, 656
Bse1I ACTGG 6 cut(s) 484, 572, 599, 650, 746, 1294
Bse21I CCTNAGG 1 cut(s) 536
Bse3DI GCAATG 1 cut(s) 84
Bse8I GATNNNNATC 1 cut(s) 69
BseBI CCWGG 1 cut(s) 604
BseGI GGATG 3 cut(s) 361, 576, 1205
BseJI GATNNNNATC 1 cut(s) 69
BseLI CCNNNNNNNGG 4 cut(s) 352, 430, 541, 656
BseMI GCAATG 1 cut(s) 84
BseMII CTCAG 4 cut(s) 165, 550, 617, 1464
BseNI ACTGG 6 cut(s) 484, 572, 599, 650, 746, 1294
BseSI GKGCMC 3 cut(s) 466, 734, 872
BseYI CCCAGC 1 cut(s) 871
BsgI GTGCAG 1 cut(s) 832
Bsh1236I CGCG 1 cut(s) 1458
BshFI GGCC 6 cut(s) 95, 345, 577, 682, 745, 936
BshNI GGYRCC 2 cut(s) 53, 1333
BsiHKAI GWGCWC 1 cut(s) 466
BslI CCNNNNNNNGG 4 cut(s) 352, 430, 541, 656
BsmAI GTCTC 5 cut(s) 5, 106, 665, 693, 1504
BsmBI CGTCTC 1 cut(s) 5
BsmI GAATGC 1 cut(s) 753
BsnI GGCC 6 cut(s) 95, 345, 577, 682, 745, 936
Bso31I GGTCTC 1 cut(s) 665
Bsp1286I GDGCHC 3 cut(s) 466, 734, 872
Bsp1407I TGTACA 1 cut(s) 849
Bsp143I GATC 6 cut(s) 338, 561, 599, 613, 646, 844
BspACI CCGC 5 cut(s) 89, 92, 407, 424, 1205
BspANI GGCC 6 cut(s) 95, 345, 577, 682, 745, 936
BspCNI CTCAG 4 cut(s) 166, 549, 618, 1465
BspFNI CGCG 1 cut(s) 1458
BspLI GGNNCC 5 cut(s) 55, 528, 601, 1335, 1355
BspMAI CTGCAG 3 cut(s) 276, 525, 1246
BspMI ACCTGC 1 cut(s) 528
BspPI GGATC 6 cut(s) 346, 556, 594, 607, 641, 852
BspT107I GGYRCC 2 cut(s) 53, 1333
BspTNI GGTCTC 1 cut(s) 665
BsrDI GCAATG 1 cut(s) 84
BsrGI TGTACA 1 cut(s) 849
BsrI ACTGG 6 cut(s) 484, 572, 599, 650, 746, 1294
BssMI GATC 6 cut(s) 338, 561, 599, 613, 646, 844
BssSI CACGAG 1 cut(s) 149
Bst2BI CACGAG 1 cut(s) 149
Bst2UI CCWGG 1 cut(s) 604
Bst4CI ACNGT 4 cut(s) 117, 461, 687, 1344
Bst6I CTCTTC 2 cut(s) 1145, 1521
BstAPI GCANNNNNTGC 1 cut(s) 1253
BstAUI TGTACA 1 cut(s) 849
BstBAI YACGTR 1 cut(s) 1185
BstC8I GCNNGC 1 cut(s) 1045
BstDEI CTNAG 4 cut(s) 174, 536, 626, 1473
BstENI CCTNNNNNAGG 1 cut(s) 539
BstF5I GGATG 3 cut(s) 361, 576, 1205
BstFNI CGCG 1 cut(s) 1458
BstH2I RGCGCY 1 cut(s) 484
BstHHI GCGC 2 cut(s) 483, 1460
BstKTI GATC 6 cut(s) 341, 564, 602, 616, 649, 847
BstMAI GTCTC 5 cut(s) 5, 106, 665, 693, 1504
BstMBI GATC 6 cut(s) 338, 561, 599, 613, 646, 844
BstMWI GCNNNNNNNGC 8 cut(s) 86, 89, 98, 268, 679, 1073, 1082, 1253
BstNI CCWGG 1 cut(s) 604
BstNSI RCATGY 1 cut(s) 185
BstSCI CCNGG 1 cut(s) 602
BstSFI CTRYAG 6 cut(s) 243, 252, 272, 521, 1242, 1461
BstSLI GKGCMC 3 cut(s) 466, 734, 872
BstUI CGCG 1 cut(s) 1458
BstV2I GAAGAC 1 cut(s) 908
BstX2I RGATCY 3 cut(s) 599, 646, 844
BstXI CCANNNNNNTGG 2 cut(s) 741, 1185
BstYI RGATCY 3 cut(s) 599, 646, 844
Bsu36I CCTNAGG 1 cut(s) 536
BsuRI GGCC 6 cut(s) 95, 345, 577, 682, 745, 936
BtgZI GCGATG 2 cut(s) 78, 1080
BtrI CACGTC 1 cut(s) 559
BtsCI GGATG 3 cut(s) 361, 576, 1205
BtsI GCAGTG 1 cut(s) 820
BtsIMutI CAGTG 6 cut(s) 466, 565, 585, 592, 683, 820
BveI ACCTGC 1 cut(s) 528
Cac8I GCNNGC 1 cut(s) 1045
CaiI CAGNNNCTG 1 cut(s) 251
CfoI GCGC 2 cut(s) 483, 1460
Cfr13I GGNCC 1 cut(s) 344
Csp6I GTAC 5 cut(s) 54, 118, 457, 850, 1271
CviQI GTAC 5 cut(s) 54, 118, 457, 850, 1271
DdeI CTNAG 4 cut(s) 174, 536, 626, 1473
DpnI GATC 6 cut(s) 340, 563, 601, 615, 648, 846
DpnII GATC 6 cut(s) 338, 561, 599, 613, 646, 844
DraI TTTAAA 1 cut(s) 490
EaeI YGGCCR 1 cut(s) 743
Eam1104I CTCTTC 2 cut(s) 1145, 1521
EarI CTCTTC 2 cut(s) 1145, 1521
Eco31I GGTCTC 1 cut(s) 665
Eco57I CTGAAG 3 cut(s) 822, 920, 1389
Eco72I CACGTG 1 cut(s) 1185
Eco81I CCTNAGG 1 cut(s) 536
EcoNI CCTNNNNNAGG 1 cut(s) 539
EcoRI GAATTC 1 cut(s) 940
EcoRII CCWGG 1 cut(s) 602
Esp3I CGTCTC 1 cut(s) 5
FalI AAGNNNNNCTT 6 cut(s) 114, 146, 297, 329, 969, 1001
FbaI TGATCA 1 cut(s) 613
FokI GGATG 3 cut(s) 368, 583, 1212
FspBI CTAG 1 cut(s) 1289
GlaI GCGC 2 cut(s) 482, 1459
GsaI CCCAGC 1 cut(s) 875
GsuI CTGGAG 4 cut(s) 312, 410, 418, 625
HaeII RGCGCY 1 cut(s) 484
HaeIII GGCC 6 cut(s) 95, 345, 577, 682, 745, 936
HhaI GCGC 2 cut(s) 483, 1460
Hin6I GCGC 2 cut(s) 481, 1458
HinP1I GCGC 2 cut(s) 481, 1458
HinfI GANTC 9 cut(s) 400, 622, 904, 970, 998, 1088, 1260, 1419, 1497
HphI GGTGA 2 cut(s) 198, 371
Hpy166II GTNNAC 3 cut(s) 464, 852, 883
Hpy188I TCNGA 6 cut(s) 106, 231, 627, 900, 1073, 1474
Hpy8I GTNNAC 3 cut(s) 464, 852, 883
HpyAV CCTTC 2 cut(s) 477, 535
HpyCH4III ACNGT 4 cut(s) 117, 461, 687, 1344
HpyCH4IV ACGT 3 cut(s) 160, 558, 1184
HpyF10VI GCNNNNNNNGC 8 cut(s) 86, 89, 98, 268, 679, 1073, 1082, 1253
HpyF3I CTNAG 4 cut(s) 174, 536, 626, 1473
HpySE526I ACGT 3 cut(s) 160, 558, 1184
HspAI GCGC 2 cut(s) 481, 1458
KpnI GGTACC 1 cut(s) 57
Ksp22I TGATCA 1 cut(s) 613
Kzo9I GATC 6 cut(s) 338, 561, 599, 613, 646, 844
LmnI GCTCC 3 cut(s) 409, 445, 1353
LweI GCATC 2 cut(s) 1051, 1398
MaeI CTAG 1 cut(s) 1289
MaeII ACGT 3 cut(s) 160, 558, 1184
MaeIII GTNAC 2 cut(s) 164, 652
MalI GATC 6 cut(s) 340, 563, 601, 615, 648, 846
MboI GATC 6 cut(s) 338, 561, 599, 613, 646, 844
MboII GAAGA 9 cut(s) 185, 828, 913, 977, 979, 1132, 1382, 1406, 1538
MfeI CAATTG 1 cut(s) 75
MflI RGATCY 3 cut(s) 599, 646, 844
MhlI GDGCHC 3 cut(s) 466, 734, 872
MlsI TGGCCA 1 cut(s) 745
MluCI AATT 9 cut(s) 75, 198, 376, 794, 819, 940, 1212, 1446, 1467
MluNI TGGCCA 1 cut(s) 745
MlyI GAGTC 4 cut(s) 409, 898, 1428, 1506
MmeI TCCRAC 2 cut(s) 129, 530
MnlI CCTC 8 cut(s) 12, 273, 385, 545, 588, 984, 1016, 1483
Mox20I TGGCCA 1 cut(s) 745
MscI TGGCCA 1 cut(s) 745
MseI TTAA 2 cut(s) 489, 1119
MslI CAYNNNNRTG 1 cut(s) 1183
Msp20I TGGCCA 1 cut(s) 745
MspA1I CMGCKG 1 cut(s) 426
MspR9I CCNGG 1 cut(s) 604
MunI CAATTG 1 cut(s) 75
Mva1269I GAATGC 1 cut(s) 753
MvaI CCWGG 1 cut(s) 604
MvnI CGCG 1 cut(s) 1458
MwoI GCNNNNNNNGC 8 cut(s) 86, 89, 98, 268, 679, 1073, 1082, 1253
NdeII GATC 6 cut(s) 338, 561, 599, 613, 646, 844
NlaIV GGNNCC 5 cut(s) 55, 528, 601, 1335, 1355
NmuCI GTSAC 1 cut(s) 164
NspI RCATGY 1 cut(s) 185
PcsI WCGNNNNNNNCGW 1 cut(s) 20
PctI GAATGC 1 cut(s) 753
PfeI GAWTC 5 cut(s) 622, 970, 998, 1088, 1260
PflFI GACNNNGTC 1 cut(s) 1496
PfoI TCCNGGA 1 cut(s) 602
PleI GAGTC 4 cut(s) 408, 898, 1427, 1505
PmaCI CACGTG 1 cut(s) 1185
PmlI CACGTG 1 cut(s) 1185
PpsI GAGTC 4 cut(s) 408, 898, 1427, 1505
Ppu21I YACGTR 1 cut(s) 1185
PshBI ATTAAT 1 cut(s) 1119
Psp6I CCWGG 1 cut(s) 602
PspCI CACGTG 1 cut(s) 1185
PspFI CCCAGC 1 cut(s) 871
PspGI CCWGG 1 cut(s) 602
PspN4I GGNNCC 5 cut(s) 55, 528, 601, 1335, 1355
PspPI GGNCC 1 cut(s) 344
PstI CTGCAG 3 cut(s) 276, 525, 1246
PstNI CAGNNNCTG 1 cut(s) 251
PsuI RGATCY 3 cut(s) 599, 646, 844
PsyI GACNNNGTC 1 cut(s) 1496
RsaI GTAC 5 cut(s) 55, 119, 458, 851, 1272
RsaNI GTAC 5 cut(s) 54, 118, 457, 850, 1271
RseI CAYNNNNRTG 1 cut(s) 1183
SaqAI TTAA 2 cut(s) 489, 1119
Sau3AI GATC 6 cut(s) 338, 561, 599, 613, 646, 844
Sau96I GGNCC 1 cut(s) 344
SbfI CCTGCAGG 1 cut(s) 525
SchI GAGTC 4 cut(s) 409, 898, 1428, 1506
ScrFI CCNGG 1 cut(s) 604
SdaI CCTGCAGG 1 cut(s) 525
SduI GDGCHC 3 cut(s) 466, 734, 872
SfaNI GCATC 2 cut(s) 1051, 1398
SfcI CTRYAG 6 cut(s) 243, 252, 272, 521, 1242, 1461
SmiMI CAYNNNNRTG 1 cut(s) 1183
SmlI CTYRAG 3 cut(s) 975, 1007, 1487
SmoI CTYRAG 3 cut(s) 975, 1007, 1487
Sse8387I CCTGCAGG 1 cut(s) 525
Sse9I AATT 9 cut(s) 75, 198, 376, 794, 819, 940, 1212, 1446, 1467
SsiI CCGC 5 cut(s) 89, 92, 407, 424, 1205
SspI AATATT 1 cut(s) 893
SspMI CTAG 1 cut(s) 1289
StyD4I CCNGG 1 cut(s) 602
TaaI ACNGT 4 cut(s) 117, 461, 687, 1344
TaiI ACGT 3 cut(s) 163, 561, 1187
TaqI TCGA 2 cut(s) 749, 1392
TasI AATT 9 cut(s) 75, 198, 376, 794, 819, 940, 1212, 1446, 1467
TatI WGTACW 1 cut(s) 849
TauI GCSGC 3 cut(s) 92, 95, 1208
TfiI GAWTC 5 cut(s) 622, 970, 998, 1088, 1260
Tru1I TTAA 2 cut(s) 489, 1119
Tru9I TTAA 2 cut(s) 489, 1119
TscAI CASTG 6 cut(s) 466, 572, 592, 599, 690, 820
TseFI GTSAC 1 cut(s) 164
Tsp45I GTSAC 1 cut(s) 164
TspDTI ATGAA 9 cut(s) 31, 192, 459, 690, 789, 801, 1080, 1205, 1398
TspGWI ACGGA 4 cut(s) 29, 407, 444, 1509
TspRI CASTG 6 cut(s) 466, 572, 592, 599, 690, 820
Tth111I GACNNNGTC 1 cut(s) 1496
VneI GTGCAC 1 cut(s) 462
VspI ATTAAT 1 cut(s) 1119
XagI CCTNNNNNAGG 1 cut(s) 539
XapI RAATTY 4 cut(s) 376, 819, 940, 1467
XceI RCATGY 1 cut(s) 185
XspI CTAG 1 cut(s) 1289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.