FvH4_5g32890

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0009185
Physical Location & Seq
Forward (+)
23750225 .. 23753994
3770 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g32890.t1

Sequence Viewer

Length: 1128 bp
ATGGCTAAGCAGTTCTACTTTAGGCTGTTTTCTTCTGGTTTGCTTGTTTTGTACTTCATGGTGCTGCAGGCTGAAGTAGCTTGGGGTCAAGGTGGTGCTGCAGAAGGGAGGAAAGAAATGGTGCCTGCAATGTTCATATTTGGAGACTCTCTGATTGACAATGGCAACAACAACAACCTCCCTTCATTTGCCAAAGCCAACTATCCGCCCTATGGAATCGATTTCAGTGGCGGACCAACTGGTCGGTTTAGCAATGGTTATACCATGGTTGATGAAATAGCTGAATTGCTAGGACTTCCCTTAATTCCTGCTTTCTCTGAAGCTACTGGAGATCAAGTCCTTCATGGTGTTAATTATGCATCTGCAGCCGCTGGAATCCTTGATATCACTGGCAGAAACTTCGTTGGTCGCATTCCTTTTGGTCAACAAATAAAGAACTTCCAGACTACACTTGATCAGATAACAGAGAACCTGGGCGCAGATGATGTTGCCCGGGCCATTGGGAAGTGCATATTCTTTGTTGGAATGGGCAGCAATGACTACCTAAACAATTACCTTATGCCCAACTATGACACCAAGAATCAATACAATGCTCAACAATTTGCTGATCTCTTGGCTCAGCAATACACTCATCATCTCACTAGGCTCTACAATCTTGGTGCTCGAAAATTTGTTATTGCTGGAGTTGGGAGGATGGGTTGCATACCAAGTATCTTAGCCCAAAGTCCAAATGGAAGCTGCTCAGAGGATGTTAATCTCCTTGTTTTACCCTTCAACGCAAATGTGAAGGCAATGATCAACAATCTTAATACCAACTTACCTGGTTCAAAGTTCATTTACATTGACATTGCTCAAATGTTTGAAGATATCGTCACCAATGCTAGAGTTTATGGATTAAGTGTTGCGAATCGTGGATGCTGTGGTATTGGGCGTAATAGAGGGCAAATAACATGTCTTCCAATGCAAACACCTTGCCCAAATCGTGACCAGTACGTGTTCTGGGATGCATTTCACCCAACCGCAGCTGTGAACATCATAATAGGCAGGAAAGCTTTCAGTGGAGACTTTAGCCAGGTTTATCCCATGAACATTCAGCAGCTTTCCACTCTTAACATTGAAACCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

376

Amino Acids

41.13

Weight (kDa)

5.82

Isoelectric Point (pI)

26.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 45 - 349 6e-35 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 240
AccB1I GGYRCC 1 cut(s) 121
AciI CCGC 4 cut(s) 206, 231, 369, 1020
AcsI RAATTY 1 cut(s) 668
AcuI CTGAAG 2 cut(s) 93, 339
AfaI GTAC 2 cut(s) 53, 992
AfiI CCNNNNNNNGG 1 cut(s) 212
AflIII ACRYGT 2 cut(s) 950, 993
AgsI TTSAA 4 cut(s) 775, 828, 863, 1118
AjnI CCWGG 3 cut(s) 471, 820, 1071
AluBI AGCT 7 cut(s) 80, 281, 323, 738, 1025, 1052, 1099
AluI AGCT 7 cut(s) 80, 281, 323, 738, 1025, 1052, 1099
Alw21I GWGCWC 1 cut(s) 664
Alw26I GTCTC 2 cut(s) 138, 1056
AlwNI CAGNNNCTG 1 cut(s) 371
Ama87I CYCGRG 1 cut(s) 492
AoxI GGCC 1 cut(s) 495
ApeKI GCWGC 7 cut(s) 64, 98, 365, 531, 738, 1022, 1096
ApoI RAATTY 1 cut(s) 668
ArsI GACNNNNNNTTYG 2 cut(s) 937, 969
Asp700I GAANNNNTTC 1 cut(s) 1052
AspLEI GCGC 1 cut(s) 479
AspS9I GGNCC 2 cut(s) 233, 495
AsuC2I CCSGG 2 cut(s) 493, 494
AsuHPI GGTGA 2 cut(s) 865, 1004
AvaI CYCGRG 1 cut(s) 492
AvaII GGWCC 1 cut(s) 233
BanI GGYRCC 1 cut(s) 121
BbsI GAAGAC 1 cut(s) 947
Bbv12I GWGCWC 1 cut(s) 664
BbvI GCAGC 7 cut(s) 51, 85, 377, 543, 725, 1034, 1108
BccI CCATC 1 cut(s) 688
BcgI CGANNNNNNTGC 2 cut(s) 382, 416
BciT130I CCWGG 3 cut(s) 473, 822, 1073
BclI TGATCA 2 cut(s) 454, 795
BcnI CCSGG 2 cut(s) 493, 494
BcoDI GTCTC 2 cut(s) 138, 1056
BfaI CTAG 3 cut(s) 290, 642, 882
BfmI CTRYAG 3 cut(s) 65, 99, 363
BisI GCNGC 8 cut(s) 65, 99, 366, 369, 532, 739, 1023, 1097
BlpI GCTNAGC 2 cut(s) 6, 618
BlsI GCNGC 8 cut(s) 66, 100, 367, 370, 533, 740, 1024, 1098
Bme1390I CCNGG 5 cut(s) 473, 493, 494, 822, 1073
Bme18I GGWCC 1 cut(s) 233
BmeT110I CYCGRG 1 cut(s) 492
BmgT120I GGNCC 2 cut(s) 233, 495
BmiI GGNNCC 1 cut(s) 123
BmrFI CCNGG 5 cut(s) 473, 493, 494, 822, 1073
BmsI GCATC 3 cut(s) 368, 905, 994
BpiI GAAGAC 1 cut(s) 947
BpmI CTGGAG 2 cut(s) 348, 702
Bpu1102I GCTNAGC 2 cut(s) 6, 618
BpuMI CCSGG 2 cut(s) 493, 494
Bsa29I ATCGAT 1 cut(s) 219
BsaAI YACGTR 1 cut(s) 994
BsaBI GATNNNNATC 1 cut(s) 753
BsaJI CCNNGG 3 cut(s) 264, 472, 492
BsaXI ACNNNNNCTCC 4 cut(s) 321, 351, 682, 712
Bsc4I CCNNNNNNNGG 1 cut(s) 212
Bse1I ACTGG 4 cut(s) 244, 331, 394, 988
Bse3DI GCAATG 5 cut(s) 135, 259, 541, 798, 846
Bse8I GATNNNNATC 1 cut(s) 753
BseBI CCWGG 3 cut(s) 473, 822, 1073
BseCI ATCGAT 1 cut(s) 219
BseDI CCNNGG 3 cut(s) 264, 472, 492
BseGI GGATG 4 cut(s) 699, 754, 920, 1009
BseJI GATNNNNATC 1 cut(s) 753
BseLI CCNNNNNNNGG 1 cut(s) 212
BseMI GCAATG 5 cut(s) 135, 259, 541, 798, 846
BseMII CTCAG 2 cut(s) 632, 756
BseNI ACTGG 4 cut(s) 244, 331, 394, 988
BseXI GCAGC 7 cut(s) 51, 85, 377, 543, 725, 1034, 1108
BshFI GGCC 1 cut(s) 497
BshNI GGYRCC 1 cut(s) 121
BshVI ATCGAT 1 cut(s) 219
BsiHKAI GWGCWC 1 cut(s) 664
BsiHKCI CYCGRG 1 cut(s) 492
BsiSI CCGG 1 cut(s) 493
BslI CCNNNNNNNGG 1 cut(s) 212
BsmAI GTCTC 2 cut(s) 138, 1056
BsmI GAATGC 1 cut(s) 411
BsnI GGCC 1 cut(s) 497
BsoBI CYCGRG 1 cut(s) 492
Bsp1286I GDGCHC 1 cut(s) 664
Bsp143I GATC 4 cut(s) 331, 454, 607, 795
Bsp1720I GCTNAGC 2 cut(s) 6, 618
Bsp19I CCATGG 1 cut(s) 264
BspACI CCGC 4 cut(s) 206, 231, 369, 1020
BspANI GGCC 1 cut(s) 497
BspCNI CTCAG 2 cut(s) 631, 755
BspDI ATCGAT 1 cut(s) 219
BspLI GGNNCC 1 cut(s) 123
BspMAI CTGCAG 3 cut(s) 69, 103, 367
BspT107I GGYRCC 1 cut(s) 121
BsrDI GCAATG 5 cut(s) 135, 259, 541, 798, 846
BsrI ACTGG 4 cut(s) 244, 331, 394, 988
BssECI CCNNGG 3 cut(s) 264, 472, 492
BssMI GATC 4 cut(s) 331, 454, 607, 795
BssT1I CCWWGG 1 cut(s) 264
Bst2UI CCWGG 3 cut(s) 473, 822, 1073
BstBAI YACGTR 1 cut(s) 994
BstC8I GCNNGC 2 cut(s) 69, 126
BstDEI CTNAG 4 cut(s) 6, 618, 715, 742
BstDSI CCRYGG 1 cut(s) 264
BstF5I GGATG 4 cut(s) 699, 754, 920, 1009
BstHHI GCGC 1 cut(s) 479
BstKTI GATC 4 cut(s) 334, 457, 610, 798
BstMAI GTCTC 2 cut(s) 138, 1056
BstMBI GATC 4 cut(s) 331, 454, 607, 795
BstMWI GCNNNNNNNGC 2 cut(s) 77, 365
BstNI CCWGG 3 cut(s) 473, 822, 1073
BstNSI RCATGY 1 cut(s) 954
BstSCI CCNGG 5 cut(s) 471, 491, 492, 820, 1071
BstSFI CTRYAG 3 cut(s) 65, 99, 363
BstV1I GCAGC 7 cut(s) 51, 85, 377, 543, 725, 1034, 1108
BstV2I GAAGAC 1 cut(s) 947
Bsu15I ATCGAT 1 cut(s) 219
BsuRI GGCC 1 cut(s) 497
BsuTUI ATCGAT 1 cut(s) 219
BtgI CCRYGG 1 cut(s) 264
BtsCI GGATG 4 cut(s) 699, 754, 920, 1009
BtsIMutI CAGTG 3 cut(s) 232, 387, 1063
Cac8I GCNNGC 2 cut(s) 69, 126
CaiI CAGNNNCTG 1 cut(s) 371
CfoI GCGC 1 cut(s) 479
Cfr13I GGNCC 2 cut(s) 233, 495
Cfr9I CCCGGG 1 cut(s) 492
ClaI ATCGAT 1 cut(s) 219
CsiI ACCWGGT 1 cut(s) 820
Csp6I GTAC 2 cut(s) 52, 991
CviAII CATG 5 cut(s) 58, 265, 344, 951, 1084
CviQI GTAC 2 cut(s) 52, 991
DdeI CTNAG 4 cut(s) 6, 618, 715, 742
DpnI GATC 4 cut(s) 333, 456, 609, 797
DpnII GATC 4 cut(s) 331, 454, 607, 795
DrdI GACNNNNNNGTC 1 cut(s) 240
DseDI GACNNNNNNGTC 1 cut(s) 240
EciI GGCGGA 2 cut(s) 195, 246
Eco130I CCWWGG 1 cut(s) 264
Eco32I GATATC 2 cut(s) 385, 868
Eco47I GGWCC 1 cut(s) 233
Eco57I CTGAAG 2 cut(s) 93, 339
Eco88I CYCGRG 1 cut(s) 492
EcoRII CCWGG 3 cut(s) 471, 820, 1071
EcoRV GATATC 2 cut(s) 385, 868
EcoT14I CCWWGG 1 cut(s) 264
EcoT22I ATGCAT 2 cut(s) 361, 1009
ErhI CCWWGG 1 cut(s) 264
FaeI CATG 5 cut(s) 61, 268, 347, 954, 1087
FatI CATG 5 cut(s) 57, 264, 343, 950, 1083
FbaI TGATCA 2 cut(s) 454, 795
Fnu4HI GCNGC 8 cut(s) 65, 99, 366, 369, 532, 739, 1023, 1097
FokI GGATG 4 cut(s) 706, 761, 927, 1016
Fsp4HI GCNGC 8 cut(s) 65, 99, 366, 369, 532, 739, 1023, 1097
FspBI CTAG 3 cut(s) 290, 642, 882
GlaI GCGC 1 cut(s) 478
GluI GCNGC 8 cut(s) 65, 99, 366, 369, 532, 739, 1023, 1097
GsuI CTGGAG 2 cut(s) 348, 702
HaeIII GGCC 1 cut(s) 497
HapII CCGG 1 cut(s) 493
HhaI GCGC 1 cut(s) 479
Hin1II CATG 5 cut(s) 61, 268, 347, 954, 1087
Hin6I GCGC 1 cut(s) 477
HinP1I GCGC 1 cut(s) 477
HincII GTYRAC 1 cut(s) 425
HindII GTYRAC 1 cut(s) 425
HindIII AAGCTT 1 cut(s) 1050
HinfI GANTC 5 cut(s) 146, 216, 375, 580, 907
HpaII CCGG 1 cut(s) 493
HphI GGTGA 2 cut(s) 865, 1004
Hpy166II GTNNAC 2 cut(s) 425, 1030
Hpy188I TCNGA 4 cut(s) 153, 319, 459, 745
Hpy188III TCNNGA 2 cut(s) 442, 983
Hpy8I GTNNAC 2 cut(s) 425, 1030
HpyAV CCTTC 5 cut(s) 98, 192, 350, 781, 781
HpyCH4IV ACGT 1 cut(s) 993
HpyCH4V TGCA 9 cut(s) 67, 101, 128, 359, 365, 510, 702, 964, 1007
HpyF10VI GCNNNNNNNGC 2 cut(s) 77, 365
HpyF3I CTNAG 4 cut(s) 6, 618, 715, 742
HpySE526I ACGT 1 cut(s) 993
Hsp92II CATG 5 cut(s) 61, 268, 347, 954, 1087
HspAI GCGC 1 cut(s) 477
Ksp22I TGATCA 2 cut(s) 454, 795
Kzo9I GATC 4 cut(s) 331, 454, 607, 795
Lsp1109I GCAGC 7 cut(s) 51, 85, 377, 543, 725, 1034, 1108
LweI GCATC 3 cut(s) 368, 905, 994
MabI ACCWGGT 1 cut(s) 820
MaeI CTAG 3 cut(s) 290, 642, 882
MaeII ACGT 1 cut(s) 993
MaeIII GTNAC 2 cut(s) 871, 983
MalI GATC 4 cut(s) 333, 456, 609, 797
MboI GATC 4 cut(s) 331, 454, 607, 795
MboII GAAGA 3 cut(s) 24, 875, 947
MhlI GDGCHC 1 cut(s) 664
MluCI AATT 7 cut(s) 284, 303, 352, 550, 599, 668, 1123
MlyI GAGTC 1 cut(s) 140
MmeI TCCRAC 1 cut(s) 502
MnlI CCTC 5 cut(s) 102, 188, 684, 739, 932
Mph1103I ATGCAT 2 cut(s) 361, 1009
MroXI GAANNNNTTC 1 cut(s) 1052
MseI TTAA 7 cut(s) 302, 351, 753, 807, 896, 1110, 1126
MspA1I CMGCKG 2 cut(s) 371, 1025
MspI CCGG 1 cut(s) 493
MspR9I CCNGG 5 cut(s) 473, 493, 494, 822, 1073
Mva1269I GAATGC 1 cut(s) 411
MvaI CCWGG 3 cut(s) 473, 822, 1073
MwoI GCNNNNNNNGC 2 cut(s) 77, 365
NciI CCSGG 2 cut(s) 493, 494
NcoI CCATGG 1 cut(s) 264
NdeII GATC 4 cut(s) 331, 454, 607, 795
NlaIII CATG 5 cut(s) 61, 268, 347, 954, 1087
NlaIV GGNNCC 1 cut(s) 123
NmuCI GTSAC 2 cut(s) 871, 983
NsiI ATGCAT 2 cut(s) 361, 1009
NspI RCATGY 1 cut(s) 954
PciI ACATGT 1 cut(s) 950
PctI GAATGC 1 cut(s) 411
PdmI GAANNNNTTC 1 cut(s) 1052
PfeI GAWTC 4 cut(s) 216, 375, 580, 907
PkrI GCNGC 8 cut(s) 66, 100, 367, 370, 533, 740, 1024, 1098
PleI GAGTC 1 cut(s) 140
PpsI GAGTC 1 cut(s) 140
Ppu21I YACGTR 1 cut(s) 994
PscI ACATGT 1 cut(s) 950
Psp6I CCWGG 3 cut(s) 471, 820, 1071
PspGI CCWGG 3 cut(s) 471, 820, 1071
PspN4I GGNNCC 1 cut(s) 123
PspPI GGNCC 2 cut(s) 233, 495
PstI CTGCAG 3 cut(s) 69, 103, 367
PstNI CAGNNNCTG 1 cut(s) 371
PvuII CAGCTG 1 cut(s) 1025
RsaI GTAC 2 cut(s) 53, 992
RsaNI GTAC 2 cut(s) 52, 991
SaqAI TTAA 7 cut(s) 302, 351, 753, 807, 896, 1110, 1126
SatI GCNGC 8 cut(s) 65, 99, 366, 369, 532, 739, 1023, 1097
Sau3AI GATC 4 cut(s) 331, 454, 607, 795
Sau96I GGNCC 2 cut(s) 233, 495
SchI GAGTC 1 cut(s) 140
ScrFI CCNGG 5 cut(s) 473, 493, 494, 822, 1073
SduI GDGCHC 1 cut(s) 664
SexAI ACCWGGT 1 cut(s) 820
SfaNI GCATC 3 cut(s) 368, 905, 994
SfcI CTRYAG 3 cut(s) 65, 99, 363
SinI GGWCC 1 cut(s) 233
SmaI CCCGGG 1 cut(s) 494
SrfI GCCCGGGC 1 cut(s) 494
Sse9I AATT 7 cut(s) 284, 303, 352, 550, 599, 668, 1123
SsiI CCGC 4 cut(s) 206, 231, 369, 1020
SspMI CTAG 3 cut(s) 290, 642, 882
StyD4I CCNGG 5 cut(s) 471, 491, 492, 820, 1071
StyI CCWWGG 1 cut(s) 264
TaiI ACGT 1 cut(s) 996
TaqI TCGA 2 cut(s) 219, 664
TasI AATT 7 cut(s) 284, 303, 352, 550, 599, 668, 1123
TatI WGTACW 1 cut(s) 51
TauI GCSGC 1 cut(s) 371
TfiI GAWTC 4 cut(s) 216, 375, 580, 907
Tru1I TTAA 7 cut(s) 302, 351, 753, 807, 896, 1110, 1126
Tru9I TTAA 7 cut(s) 302, 351, 753, 807, 896, 1110, 1126
TscAI CASTG 3 cut(s) 232, 394, 1063
TseFI GTSAC 2 cut(s) 871, 983
TseI GCWGC 7 cut(s) 64, 98, 365, 531, 738, 1022, 1096
Tsp45I GTSAC 2 cut(s) 871, 983
TspDTI ATGAA 7 cut(s) 46, 124, 174, 288, 332, 823, 1100
TspMI CCCGGG 1 cut(s) 492
TspRI CASTG 3 cut(s) 232, 394, 1063
VpaK11BI GGWCC 1 cut(s) 233
XapI RAATTY 1 cut(s) 668
XceI RCATGY 1 cut(s) 954
XcmI CCANNNNNNNNNTGG 1 cut(s) 728
XmaI CCCGGG 1 cut(s) 492
XmnI GAANNNNTTC 1 cut(s) 1052
XspI CTAG 3 cut(s) 290, 642, 882
Zsp2I ATGCAT 2 cut(s) 361, 1009
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.