Rh7AG415800

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0009185
Physical Location & Seq
Forward (+)
55859946 .. 55864441
4496 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG415800.1

Sequence Viewer

Length: 864 bp
ATGGTTGATGAAATAGCTGAATTGCTAGGACTTCCTTTAATTCCTGCGTACTCTGAAGCTTCTGGAGATCAAGTGCTTCATGGTGTCAATTATGCATCTGCAGCCGCTGGAATCCTTGATATCACTGGCAGAAACTTTGTGGGTCGCATACCCTTTGGTCAACAAATAAGCAACTTCCAGACTACAGTTGATCAGATAACGGAGACTCTGGGTGCAGATGATGTTGCCCGGGCCATTGCAAAGTGCATATTCTTTGTTGGAATGGGCAGCAATGACTACCTAAACAATTACCTTATGCCCAACTATAACACCAAGAATCAATACAATGCTCAACAATTTGCTGATCTCTTGGCTCAACAATACACTCAGCAACTCACTAGGCTCTACAATCTTGGTGCTCGAAGATTTGTTATTGCTGGAGTTGGGAGGATGGGTTGCATACCAAGTATCTTAGCCCAAAGTCCAAATGGAAGCTGCTCAGAGGACGTCAATCGCCTTGTTCTACCCTTCAACGCAAATGTGAAGACAATGATCAACAATCTTAATACCAACTTACCTGGTTCGAGGTTCATTTACATTGACATTGCTCGAATGTTTGAAGACGTCGTCACCAATGCTAGAGCTTATGGATTCAGTGTTGCGAATCGTGGATGCTGTGGTATTGGGCGTAACAGAGGGCAAATTACATGTCTTCCAATGCAAACACCGTGCCCAAATCGTGACCAGTACGTGTTCTGGGATGCATATCACCCAACTGCAGCTGTGAACATCTTAATTGGCAGGAAAGCTTTCAGTGGAGACCCTAGCCAGGTTTACCCCATGAACATACAGCAGCTTGCCACTCTTAACATTGAAACCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

287

Amino Acids

31.54

Weight (kDa)

6.11

Isoelectric Point (pI)

30.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 3 - 260 2.2e-27 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 489, 606
AciI CCGC 1 cut(s) 105
AcuI CTGAAG 1 cut(s) 75
AcyI GRCGYC 2 cut(s) 486, 603
AfaI GTAC 2 cut(s) 50, 728
AfiI CCNNNNNNNGG 1 cut(s) 808
AflIII ACRYGT 2 cut(s) 686, 729
AgsI TTSAA 3 cut(s) 511, 599, 854
AjnI CCWGG 2 cut(s) 556, 807
AluBI AGCT 7 cut(s) 17, 59, 474, 623, 761, 788, 835
AluI AGCT 7 cut(s) 17, 59, 474, 623, 761, 788, 835
Alw21I GWGCWC 1 cut(s) 400
Alw26I GTCTC 2 cut(s) 197, 792
AlwNI CAGNNNCTG 1 cut(s) 107
Ama87I CYCGRG 1 cut(s) 228
AoxI GGCC 1 cut(s) 231
ApeKI GCWGC 5 cut(s) 101, 267, 474, 758, 832
ArsI GACNNNNNNTTYG 2 cut(s) 673, 705
Asp700I GAANNNNTTC 1 cut(s) 788
AspS9I GGNCC 1 cut(s) 231
AsuC2I CCSGG 2 cut(s) 229, 230
AsuHPI GGTGA 2 cut(s) 601, 740
AvaI CYCGRG 1 cut(s) 228
BaeGI GKGCMC 1 cut(s) 713
BbsI GAAGAC 3 cut(s) 530, 606, 683
Bbv12I GWGCWC 1 cut(s) 400
BbvI GCAGC 5 cut(s) 113, 279, 461, 770, 844
BccI CCATC 1 cut(s) 424
BciT130I CCWGG 2 cut(s) 558, 809
BclI TGATCA 2 cut(s) 190, 531
BcnI CCSGG 2 cut(s) 229, 230
BcoDI GTCTC 2 cut(s) 197, 792
BfaI CTAG 4 cut(s) 26, 378, 618, 804
BfmI CTRYAG 3 cut(s) 99, 183, 756
BisI GCNGC 6 cut(s) 102, 105, 268, 475, 759, 833
BlsI GCNGC 6 cut(s) 103, 106, 269, 476, 760, 834
Bme1390I CCNGG 4 cut(s) 229, 230, 558, 809
BmeT110I CYCGRG 1 cut(s) 228
BmgT120I GGNCC 1 cut(s) 231
BmrFI CCNGG 4 cut(s) 229, 230, 558, 809
BmsI GCATC 3 cut(s) 104, 641, 730
BpiI GAAGAC 3 cut(s) 530, 606, 683
BpmI CTGGAG 2 cut(s) 84, 438
BpuMI CCSGG 2 cut(s) 229, 230
BsaAI YACGTR 1 cut(s) 730
BsaBI GATNNNNATC 1 cut(s) 744
BsaHI GRCGYC 2 cut(s) 486, 603
BsaI GGTCTC 1 cut(s) 792
BsaJI CCNNGG 1 cut(s) 228
BsaXI ACNNNNNCTCC 4 cut(s) 57, 87, 418, 448
Bsc4I CCNNNNNNNGG 1 cut(s) 808
Bse1I ACTGG 2 cut(s) 130, 724
Bse3DI GCAATG 3 cut(s) 234, 277, 582
Bse8I GATNNNNATC 1 cut(s) 744
BseBI CCWGG 2 cut(s) 558, 809
BseDI CCNNGG 1 cut(s) 228
BseGI GGATG 3 cut(s) 435, 656, 745
BseJI GATNNNNATC 1 cut(s) 744
BseLI CCNNNNNNNGG 1 cut(s) 808
BseMI GCAATG 3 cut(s) 234, 277, 582
BseMII CTCAG 2 cut(s) 380, 492
BseNI ACTGG 2 cut(s) 130, 724
BseSI GKGCMC 1 cut(s) 713
BseXI GCAGC 5 cut(s) 113, 279, 461, 770, 844
BsgI GTGCAG 1 cut(s) 234
BshFI GGCC 1 cut(s) 233
BsiHKAI GWGCWC 1 cut(s) 400
BsiHKCI CYCGRG 1 cut(s) 228
BsiSI CCGG 1 cut(s) 229
BslI CCNNNNNNNGG 1 cut(s) 808
BsmAI GTCTC 2 cut(s) 197, 792
BsnI GGCC 1 cut(s) 233
Bso31I GGTCTC 1 cut(s) 792
BsoBI CYCGRG 1 cut(s) 228
Bsp1286I GDGCHC 2 cut(s) 400, 713
Bsp143I GATC 4 cut(s) 67, 190, 343, 531
BspACI CCGC 1 cut(s) 105
BspANI GGCC 1 cut(s) 233
BspCNI CTCAG 2 cut(s) 379, 491
BspMAI CTGCAG 2 cut(s) 103, 760
BspTNI GGTCTC 1 cut(s) 792
BsrDI GCAATG 3 cut(s) 234, 277, 582
BsrI ACTGG 2 cut(s) 130, 724
BssECI CCNNGG 1 cut(s) 228
BssMI GATC 4 cut(s) 67, 190, 343, 531
BssNI GRCGYC 2 cut(s) 486, 603
Bst2UI CCWGG 2 cut(s) 558, 809
Bst4CI ACNGT 2 cut(s) 187, 708
BstACI GRCGYC 2 cut(s) 486, 603
BstBAI YACGTR 1 cut(s) 730
BstC8I GCNNGC 1 cut(s) 837
BstDEI CTNAG 3 cut(s) 366, 451, 478
BstF5I GGATG 3 cut(s) 435, 656, 745
BstKTI GATC 4 cut(s) 70, 193, 346, 534
BstMAI GTCTC 2 cut(s) 197, 792
BstMBI GATC 4 cut(s) 67, 190, 343, 531
BstMWI GCNNNNNNNGC 1 cut(s) 101
BstNI CCWGG 2 cut(s) 558, 809
BstNSI RCATGY 1 cut(s) 690
BstSCI CCNGG 4 cut(s) 227, 228, 556, 807
BstSFI CTRYAG 3 cut(s) 99, 183, 756
BstSLI GKGCMC 1 cut(s) 713
BstV1I GCAGC 5 cut(s) 113, 279, 461, 770, 844
BstV2I GAAGAC 3 cut(s) 530, 606, 683
BsuRI GGCC 1 cut(s) 233
BtsCI GGATG 3 cut(s) 435, 656, 745
BtsIMutI CAGTG 3 cut(s) 123, 640, 799
Cac8I GCNNGC 1 cut(s) 837
CaiI CAGNNNCTG 1 cut(s) 107
Cfr13I GGNCC 1 cut(s) 231
Cfr9I CCCGGG 1 cut(s) 228
CsiI ACCWGGT 1 cut(s) 556
Csp6I GTAC 2 cut(s) 49, 727
CviAII CATG 3 cut(s) 80, 687, 820
CviQI GTAC 2 cut(s) 49, 727
DdeI CTNAG 3 cut(s) 366, 451, 478
DpnI GATC 4 cut(s) 69, 192, 345, 533
DpnII GATC 4 cut(s) 67, 190, 343, 531
Eco31I GGTCTC 1 cut(s) 792
Eco32I GATATC 1 cut(s) 121
Eco57I CTGAAG 1 cut(s) 75
Eco88I CYCGRG 1 cut(s) 228
EcoRII CCWGG 2 cut(s) 556, 807
EcoRV GATATC 1 cut(s) 121
EcoT22I ATGCAT 2 cut(s) 97, 745
FaeI CATG 3 cut(s) 83, 690, 823
FatI CATG 3 cut(s) 79, 686, 819
FbaI TGATCA 2 cut(s) 190, 531
Fnu4HI GCNGC 6 cut(s) 102, 105, 268, 475, 759, 833
FokI GGATG 3 cut(s) 442, 663, 752
Fsp4HI GCNGC 6 cut(s) 102, 105, 268, 475, 759, 833
FspBI CTAG 4 cut(s) 26, 378, 618, 804
GluI GCNGC 6 cut(s) 102, 105, 268, 475, 759, 833
GsuI CTGGAG 2 cut(s) 84, 438
HaeIII GGCC 1 cut(s) 233
HapII CCGG 1 cut(s) 229
Hin1I GRCGYC 2 cut(s) 486, 603
Hin1II CATG 3 cut(s) 83, 690, 823
HincII GTYRAC 1 cut(s) 161
HindII GTYRAC 1 cut(s) 161
HindIII AAGCTT 2 cut(s) 57, 786
HinfI GANTC 5 cut(s) 111, 205, 316, 630, 643
HpaII CCGG 1 cut(s) 229
HphI GGTGA 2 cut(s) 601, 740
Hpy166II GTNNAC 3 cut(s) 161, 766, 814
Hpy188I TCNGA 3 cut(s) 55, 195, 481
Hpy188III TCNNGA 3 cut(s) 63, 178, 719
Hpy8I GTNNAC 3 cut(s) 161, 766, 814
Hpy99I CGWCG 1 cut(s) 608
HpyAV CCTTC 1 cut(s) 517
HpyCH4III ACNGT 2 cut(s) 187, 708
HpyCH4IV ACGT 3 cut(s) 486, 603, 729
HpyCH4V TGCA 9 cut(s) 95, 101, 215, 239, 246, 438, 700, 743, 758
HpyF10VI GCNNNNNNNGC 1 cut(s) 101
HpyF3I CTNAG 3 cut(s) 366, 451, 478
HpySE526I ACGT 3 cut(s) 486, 603, 729
Hsp92I GRCGYC 2 cut(s) 486, 603
Hsp92II CATG 3 cut(s) 83, 690, 823
Ksp22I TGATCA 2 cut(s) 190, 531
Kzo9I GATC 4 cut(s) 67, 190, 343, 531
Lsp1109I GCAGC 5 cut(s) 113, 279, 461, 770, 844
LweI GCATC 3 cut(s) 104, 641, 730
MabI ACCWGGT 1 cut(s) 556
MaeI CTAG 4 cut(s) 26, 378, 618, 804
MaeII ACGT 3 cut(s) 486, 603, 729
MaeIII GTNAC 3 cut(s) 607, 668, 719
MalI GATC 4 cut(s) 69, 192, 345, 533
MboI GATC 4 cut(s) 67, 190, 343, 531
MboII GAAGA 4 cut(s) 414, 535, 611, 683
MhlI GDGCHC 2 cut(s) 400, 713
MluCI AATT 8 cut(s) 20, 39, 88, 286, 335, 681, 774, 859
MlyI GAGTC 1 cut(s) 199
MmeI TCCRAC 1 cut(s) 238
MnlI CCTC 4 cut(s) 420, 475, 558, 668
Mph1103I ATGCAT 2 cut(s) 97, 745
MroXI GAANNNNTTC 1 cut(s) 788
MseI TTAA 4 cut(s) 38, 543, 773, 846
MspA1I CMGCKG 2 cut(s) 107, 761
MspI CCGG 1 cut(s) 229
MspR9I CCNGG 4 cut(s) 229, 230, 558, 809
MvaI CCWGG 2 cut(s) 558, 809
MwoI GCNNNNNNNGC 1 cut(s) 101
NciI CCSGG 2 cut(s) 229, 230
NdeII GATC 4 cut(s) 67, 190, 343, 531
NlaIII CATG 3 cut(s) 83, 690, 823
NmuCI GTSAC 2 cut(s) 607, 719
NsiI ATGCAT 2 cut(s) 97, 745
NspI RCATGY 1 cut(s) 690
PciI ACATGT 1 cut(s) 686
PdmI GAANNNNTTC 1 cut(s) 788
PfeI GAWTC 4 cut(s) 111, 316, 630, 643
PflFI GACNNNGTC 1 cut(s) 605
PkrI GCNGC 6 cut(s) 103, 106, 269, 476, 760, 834
PleI GAGTC 1 cut(s) 199
PpsI GAGTC 1 cut(s) 199
Ppu21I YACGTR 1 cut(s) 730
PscI ACATGT 1 cut(s) 686
Psp6I CCWGG 2 cut(s) 556, 807
PspGI CCWGG 2 cut(s) 556, 807
PspPI GGNCC 1 cut(s) 231
PstI CTGCAG 2 cut(s) 103, 760
PstNI CAGNNNCTG 1 cut(s) 107
PsyI GACNNNGTC 1 cut(s) 605
PvuII CAGCTG 1 cut(s) 761
RsaI GTAC 2 cut(s) 50, 728
RsaNI GTAC 2 cut(s) 49, 727
SaqAI TTAA 4 cut(s) 38, 543, 773, 846
SatI GCNGC 6 cut(s) 102, 105, 268, 475, 759, 833
Sau3AI GATC 4 cut(s) 67, 190, 343, 531
Sau96I GGNCC 1 cut(s) 231
SchI GAGTC 1 cut(s) 199
ScrFI CCNGG 4 cut(s) 229, 230, 558, 809
SduI GDGCHC 2 cut(s) 400, 713
SexAI ACCWGGT 1 cut(s) 556
SfaNI GCATC 3 cut(s) 104, 641, 730
SfcI CTRYAG 3 cut(s) 99, 183, 756
SmaI CCCGGG 1 cut(s) 230
SrfI GCCCGGGC 1 cut(s) 230
Sse9I AATT 8 cut(s) 20, 39, 88, 286, 335, 681, 774, 859
SsiI CCGC 1 cut(s) 105
SspMI CTAG 4 cut(s) 26, 378, 618, 804
StyD4I CCNGG 4 cut(s) 227, 228, 556, 807
TaaI ACNGT 2 cut(s) 187, 708
TaiI ACGT 3 cut(s) 489, 606, 732
TaqI TCGA 3 cut(s) 400, 563, 589
TasI AATT 8 cut(s) 20, 39, 88, 286, 335, 681, 774, 859
TauI GCSGC 1 cut(s) 107
TfiI GAWTC 4 cut(s) 111, 316, 630, 643
Tru1I TTAA 4 cut(s) 38, 543, 773, 846
Tru9I TTAA 4 cut(s) 38, 543, 773, 846
TscAI CASTG 3 cut(s) 130, 640, 799
TseFI GTSAC 2 cut(s) 607, 719
TseI GCWGC 5 cut(s) 101, 267, 474, 758, 832
Tsp45I GTSAC 2 cut(s) 607, 719
TspDTI ATGAA 4 cut(s) 24, 68, 559, 836
TspGWI ACGGA 1 cut(s) 215
TspMI CCCGGG 1 cut(s) 228
TspRI CASTG 3 cut(s) 130, 640, 799
Tth111I GACNNNGTC 1 cut(s) 605
XceI RCATGY 1 cut(s) 690
XcmI CCANNNNNNNNNTGG 1 cut(s) 464
XmaI CCCGGG 1 cut(s) 228
XmnI GAANNNNTTC 1 cut(s) 788
XspI CTAG 4 cut(s) 26, 378, 618, 804
ZraI GACGTC 2 cut(s) 487, 604
Zsp2I ATGCAT 2 cut(s) 97, 745
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.